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Protein-lipid

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https://www.readbyqxmd.com/read/28095639/early-involvement-of-lysosome-dysfunction-in-the-degeneration-of-cerebral-cortical-neurons-caused-by-the-lipid-peroxidation-product-4-hydroxynonenal
#1
Shi Zhang, Erez Eitan, Mark P Mattson
Free radical-mediated oxidative damage to proteins, lipids and DNA occurs in neurons during acute brain injuries and in neurodegenerative disorders. Membrane lipid peroxidation contributes to neuronal dysfunction and death, in part by disrupting neuronal ion homeostasis and cellular bioenergetics. Emerging findings suggest that 4-hydroxynonenal (HNE), an aldehyde produced during lipid peroxidation, impairs the function of various proteins involved in neuronal homeostasis. Here we tested the hypothesis that HNE impairs the cellular system that removes damaged proteins and organelles, the autophagy - lysosome pathway in rat primary cortical neurons...
January 17, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28092439/molecular-details-of-the-ph-domain-of-acap1-bar-ph-protein-binding-to-pip-containing-membrane
#2
Chun Chan, Lanyuan Lu, Fei Sun, Jun Fan
ACAP1 proteins were previously reported to specifically bind PIP2-containing cell membranes and form well-structured protein lattices in order to conduct membrane tubulation. We carried out molecular dynamics simulations to characterize orientation of the PH domains with respect to the BAR domains inside the protein dimer. Followed by molecular dynamics simulations, we present a comprehensive orientation analysis of PH domain under different states including unbound and bound with lipids. We have examined two binding pockets on the PH domain and present PMF profiles of the two pockets to account for their preference to PIP2 lipids...
January 16, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28088021/sanguinarine-induced-oxidative-stress-and-apoptosis-like-programmed-cell-death-al-pcd-in-root-meristem-cells-of-allium-cepa
#3
Aneta Żabka, Konrad Winnicki, Justyna Teresa Polit, Janusz Maszewski
A vast number of studies on plant cell systems clearly indicate that various biotic and abiotic stresses give rise to the uncontrolled increase in the level of reactive oxygen species (ROS). Excess concentrations of ROS result in damage to proteins, lipids, carbohydrates, and DNA, which may lead, in consequence, to the apoptotic cell death. The current study investigates the effects of sanguinarine (SAN), a natural alkaloid derived from the roots of Sanguinaria canadensis, on root apical meristem cells of Allium cepa...
January 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28087332/exo-mfa-a-13c-metabolic-flux-analysis-to-dissect-tumor-microenvironment-secreted-exosome-contributions-towards-cancer-cell-metabolism
#4
Abhinav Achreja, Hongyun Zhao, Lifeng Yang, Tae Hyun Yun, Juan Marini, Deepak Nagrath
Dissecting the pleiotropic roles of tumor micro-environment (TME) on cancer progression has been brought to the foreground of research on cancer pathology. Extracellular vesicles such as exosomes, transport proteins, lipids, and nucleic acids, to mediate intercellular communication between TME components and have emerged as candidates for anti-cancer therapy. We previously reported that cancer-associated fibroblast (CAF) derived exosomes (CDEs) contain metabolites in their cargo that are utilized by cancer cells for central carbon metabolism and promote cancer growth...
January 10, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28077297/exosomes-key-mediators-of-metastasis-and-pre-metastatic-niche-formation
#5
REVIEW
Richard J Lobb, Luize G Lima, Andreas Möller
While tumour cells are classically known to communicate via direct cell-to-cell contact and the secretion of soluble protein-based factors such as cytokines and growth factors, alternative novel mechanisms that promote tumour progression have recently emerged. Now, new critical components of the secretome thought to be involved in tumour progression are exosomes, small vesicles of endocytic origin that carry a variety of bioactive molecules, including proteins, lipids, RNA, as well as DNA molecules. Cancer cell-derived exosomes have been shown to participate in crucial steps of metastatic spread of a primary tumour, ranging from oncogenic reprogramming of malignant cells to formation of pre-metastatic niches...
January 8, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28077194/the-response-of-weaned-piglets-to-dietary-valine-and-leucine
#6
F Meyer, C Jansen van Rensburg, R M Gous
Valine (Val) is considered to be the fifth-limiting amino acid in a maize-soyabean meal diet for pigs. Excess leucine (Leu) levels often occur in commercial diets, which may attenuate the effect of Val deficiency because of an increased oxidation of Val. The objective of the present experiment was to determine the effect of increasing concentrations of Leu on the response of young piglets to dietary Val. In all, 75 Large White×Landrace entire male pigs, 44 days of age and with a mean starting weight of 13...
January 12, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28063497/pld-protein-protein-interactions-with-signaling-molecules-and-modulation-by-pa
#7
J Gomez-Cambronero, A J Morris, K M Henkels
We describe methods for studying phospholipase D (PLD) interactions with signaling proteins and modulation of these interactions by the PLD reaction product, phosphatidic acid (PA). PLD is fundamental to the physiological maintenance of cellular/intracellular membranes, protein trafficking, cytoskeletal dynamics, membrane remodeling, cell proliferation, meiotic division and sporulation. PA is an acidic phospholipid involved in the biosynthesis of many other lipids that affects the enzymatic activities of many different signaling proteins via protein-lipid interactions or as a substrate...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28063489/using-hydrogen-deuterium-exchange-mass-spectrometry-to-examine-protein-membrane-interactions
#8
O Vadas, M L Jenkins, G L Dornan, J E Burke
Many fundamental cellular processes are controlled via assembly of a network of proteins at membrane surfaces. The proper recruitment of proteins to membranes can be controlled by a wide variety of mechanisms, including protein lipidation, protein-protein interactions, posttranslational modifications, and binding to specific lipid species present in membranes. There are, however, only a limited number of analytical techniques that can study the assembly of protein-membrane complexes at the molecular level. A relatively new addition to the set of techniques available to study these protein-membrane systems is the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS)...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28056409/efficacy-of-metformin-in-human-single-hair-fibre-by-atr-ftir-spectroscopy-coupled-with-statistical-analysis
#9
Kamatchi Sundaramoorthi, Gunasekaran Sethu, Sailatha Ethirajulu, Pavithra Raja Marthandam
Diabetes mellitus is chronic metabolic disorder, resulting from insulin deficiency, characterized by hyperglycemia altered metabolism of carbohydrates, proteins and lipids and an increased risk of vascular complications. There are different classes of anti-diabetic drugs in allopathic system of medicine. Metformin (dimethyl biguanide) is a blood glucose lowering agent used in the treatment of non-insulin dependent diabetes mellitus. Almost in all diseases the blood serves as the primary metabolic transport system in the body...
December 27, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28049590/inhibitory-potential-of-flavonoids-on-ptdins-3-4-5-p3-binding-with-the-phosphoinositide-dependent-kinase-1-pleckstrin-homology-domain
#10
Yelin Kang, Bong-Gyu Kim, Sunghoon Kim, Youngshim Lee, Youngdae Yoon
Many membrane-associated proteins are involved in various signaling pathways, including the phosphoinositide 3-kinase (PI3K) pathway, which has key roles in diverse cellular processes. Disruption of the activities of these proteins is involved in the development of disease in humans, making these proteins promising targets for drug development. In most cases, the catalytic domain is targeted; however, it is also possible to target membrane associations in order to regulate protein activity. In this study, we established a novel method to study protein-lipid interactions and screened for flavonoid-derived antagonists of PtdIns(3,4,5)P3 binding with the phosphoinositide-dependent kinase 1 (PDK1) pleckstrin homology (PH) domain...
December 23, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28040803/oncogenic-regulation-of-tumor-metabolic-reprogramming
#11
Míriam Tarrado-Castellarnau, Pedro de Atauri, Marta Cascante
Development of malignancy is accompanied by a complete metabolic reprogramming closely related to the acquisition of most of cancer hallmarks. In fact, key oncogenic pathways converge to adapt the metabolism of carbohydrates, proteins, lipids and nucleic acids to the dynamic tumor microenvironment, conferring a selective advantage to cancer cells. Therefore, metabolic properties of tumor cells are significantly different from those of non-transformed cells. In addition, tumor metabolic reprogramming is linked to drug resistance in cancer treatment...
September 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/28039861/uvb-generated-microvesicle-particles-a-novel-pathway-by-which-a-skin-specific-stimulus-could-exert-systemic-effects
#12
Katherine Fahy, Langni Liu, Christine M Rapp, Christina Borchers, Ji C Bihl, Yanfang Chen, Richard Simman, Jeffrey B Travers
Ultraviolet B radiation (UVB) exerts profound effects on human skin. Much is known regarding the ability of UVB to generate a plethora of bioactive agents ranging from cytokines and other bioactive proteins, lipid mediators and micro-RNAs. It is presumed that these agents are in large part responsible for the effects of UVB, which only is absorbed appreciably in the epidermis. However, the exact mechanism by which these bioactive agents can leave the epidermis are as yet unclear. This review addresses the potential role of microvesicle particles (MVP) as UVB signaling agents through transmitting biologic mediators...
December 31, 2016: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28024148/solution-structure-of-discoidal-high-density-lipoprotein-particles-with-a-shortened-apolipoprotein-a-i
#13
Stefan Bibow, Yevhen Polyhach, Cédric Eichmann, Celestine N Chi, Julia Kowal, Stefan Albiez, Robert A McLeod, Henning Stahlberg, Gunnar Jeschke, Peter Güntert, Roland Riek
High-density lipoprotein (HDL) particles are cholesterol and lipid transport containers. Mature HDL particles destined for the liver develop through the formation of intermediate discoidal HDL particles, which are the primary acceptors for cholesterol. Here we present the three-dimensional structure of reconstituted discoidal HDL (rdHDL) particles, using a shortened construct of human apolipoprotein A-I, determined from a combination of nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) and transmission electron microscopy (TEM) data...
December 26, 2016: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28011688/cyantraniliprole-at-sublethal-dosages-negatively-affects-the-development-reproduction-and-nutrient-utilization-of-ostrinia-furnacalis-lepidoptera-crambidae
#14
Chunmei Xu, Jinfeng Ding, Yunhe Zhao, Jian Luo, Wei Mu, Zhengqun Zhang
To better understand the application prospect of cyantraniliprole against the Asian corn borer, Ostrinia furnacalis, a diet-incorporation bioassay was adopted to determine the toxicity of cyantraniliprole against the fourth instar of O. furnacalis Moreover, other experiments were conducted to examine effects of sublethal levels of cyantraniliprole on larval development, nutrient utilization, and reproduction. In this study, cyantraniliprole showed a high toxicity to fourth-instar larvae of O. furnacalis at dosages of 0...
December 23, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28011684/effects-of-food-quality-on-biology-and-physiological-traits-of-sitotroga-cerealella-lepidoptera-gelechiidae
#15
E Borzoui, B Naseri, G Nouri-Ganbalani
Biology and physiological traits of Sitotroga cerealella Olivier, a world-wide insect pest of cereals, were investigated on different grains (barley, maize, rye, sorghum, triticale, and wheat). Larval and pupal duration was the shortest on wheat and triticale, and the longest on sorghum. There were significant differences in survival rate of immature stages on grains with different seed hardness. The highest realized fecundity and egg fertility was observed on triticale and the lowest was seen on sorghum. Larvae fed on triticale and wheat showed higher amount of α-amylase activity than larvae fed on other grains...
December 23, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28005966/higher-leptin-but-not-human-milk-macronutrient-concentration-distinguishes-normal-weight-from-obese-mothers-at-1-month-postpartum
#16
Arnaud De Luca, Marine Frasquet-Darrieux, Marie-Agnès Gaud, Patricia Christin, Clair-Yves Boquien, Christine Millet, Manon Herviou, Dominique Darmaun, Richard J Robins, Pierre Ingrand, Régis Hankard
INTRODUCTION: Exclusively breastfed infants born to obese mothers have previously been shown to gain less weight by 1-month postpartum than infants of normal-weight mothers. Our hypothesis is that human milk composition and volume may differ between obese and normal-weight mothers. OBJECTIVE: To compare human milk leptin, macronutrient concentration, and volume in obese and normal-weight mothers. Mother and infant characteristics were studied as secondary aims. MATERIALS AND METHODS: This cross-sectional observational study compared 50 obese mothers matched for age, parity, ethnic origin, and educational level with 50 normal-weight mothers...
2016: PloS One
https://www.readbyqxmd.com/read/28003804/golden-needle-mushroom-a-culinary-medicine-with-evidenced-based-biological-activities-and-health-promoting-properties
#17
REVIEW
Calyn Tang, Pearl Ching-Xin Hoo, Loh Teng-Hern Tan, Priyia Pusparajah, Tahir Mehmood Khan, Learn-Han Lee, Bey-Hing Goh, Kok-Gan Chan
Flammulina velutipes (enoki, velvet shank, golden needle mushroom or winter mushroom), one of the main edible mushrooms on the market, has long been recognized for its nutritional value and delicious taste. In recent decades, research has expanded beyond detailing its nutritional composition and delved into the biological activities and potential health benefits of its constituents. Many bioactive constituents from a range of families have been isolated from different parts of the mushroom, including carbohydrates, protein, lipids, glycoproteins, phenols, and sesquiterpenes...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27999992/c-type-lectins-their-network-and-roles-in-pathogen-recognition-and-immunity
#18
REVIEW
Sabine Mayer, Marie-Kristin Raulf, Bernd Lepenies
C-type lectins (CTLs) represent the most complex family of animal/human lectins that comprises 17 different groups. During evolution, CTLs have developed by diversification to cover a broad range of glycan ligands. However, ligand binding by CTLs is not necessarily restricted to glycans as some CTLs also bind to proteins, lipids, inorganic molecules, or ice crystals. CTLs share a common fold that harbors a Ca(2+) for contact to the sugar and about 18 invariant residues in a phylogenetically conserved pattern...
December 20, 2016: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/27997867/multivariate-analysis-of-fatty-acid-and-biochemical-constitutes-of-seaweeds-to-characterize-their-potential-as-bioresource-for-biofuel-and-fine-chemicals
#19
Priyanka Verma, Manoj Kumar, Girish Mishra, Dinabandhu Sahoo
In the present study bio prospecting of thirty seaweeds from Indian coasts was analyzed for their biochemical components including pigments, fatty acid and ash content. Multivariate analysis of biochemical components and fatty acids was done using Principal Component Analysis (PCA) and Agglomerative hierarchical clustering (AHC) to manifest chemotaxonomic relationship among various seaweeds. The overall analysis suggests that these seaweeds have multi-functional properties and can be utilized as promising bioresource for proteins, lipids, pigments and carbohydrates for the food/feed and biofuel industry...
November 15, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27988008/microwave-as-skin-permeation-enhancer-for-transdermal-drug-delivery-of-chitosan-5-fluorouracil-nanoparticles
#20
Asif Nawaz, Tin Wui Wong
This study investigated transdermal drug delivery mechanisms of chitosan nanoparticles with the synergistic action of microwave in skin modification. Chitosan nanoparticles, with free or conjugated 5-fluorouracil, were prepared by nanospray-drying technique. Their transdermal drug delivery profiles across untreated and microwave-treated skins (2450MHz 5min, 5+5min; 3985MHz 5min) were examined. Both constituent materials of nanoparticles and drug encapsulation were required to succeed transdermal drug delivery...
February 10, 2017: Carbohydrate Polymers
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