Elisa Gonçalves Medeiros, Michele Ramos Valente, Leandro Honorato, Marina da Silva Ferreira, Susana Ruiz Mendoza, Diego de Souza Gonçalves, Lucas Martins Alcântara, Kamilla Xavier Gomes, Marcia Ribeiro Pinto, Ernesto S Nakayasu, Geremy Clair, Isadora Filipaki Munhoz da Rocha, Flavia C G Dos Reis, Marcio L Rodrigues, Lysangela R Alves, Leonardo Nimrichter, Arturo Casadevall, Allan Jefferson Guimarães
We conducted a comprehensive comparative analysis of extracellular vesicles (EVs) from two Acanthamoeba castellanii strains, Neff (environmental) and T4 (clinical). Morphological analysis via transmission electron microscopy revealed slightly larger Neff EVs (average = 194.5 nm) compared to more polydisperse T4 EVs (average = 168.4 nm). Nanoparticle tracking analysis (NTA) and dynamic light scattering validated these differences. Proteomic analysis of the EVs identified 1,352 proteins, with 1,107 common, 161 exclusive in Neff, and 84 exclusively in T4 EVs...
May 8, 2024: MSystems