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https://www.readbyqxmd.com/read/29452381/a-fungal-argonaute-interferes-with-rna-interference
#1
Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki
No abstract text is available yet for this article.
February 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29452249/expression-and-therapeutic-implications-of-cyclin-dependent-kinase-4-cdk4-in-osteosarcoma
#2
Yubing Zhou, Jacson K Shen, Zujiang Yu, Francis J Hornicek, Quancheng Kan, Zhenfeng Duan
Overexpression and/or hyperactivation of cyclin-dependent kinase 4 (CDK4) has been found in many types of human cancers, and a CDK4 specific inhibitor, palbociclib, has been recently approved by the FDA for the treatment of breast cancer. However, the expression and the therapeutic potential of CDK4 in osteosarcoma remain unclear. In the present study, CDK4 was found to be highly expressed in human osteosarcoma tissues and cell lines as compared with normal human osteoblasts. Elevated CDK4 expression correlated with metastasis potential and poor prognosis in osteosarcoma patients as determined by immunohistochemical analysis in a human osteosarcoma tissue microarray (TMA)...
February 13, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29452160/orchiectomy-and-letrozole-differentially-regulate-synaptic-plasticity-and-spatial-memory-in-a-manner-that-is-mediated-by-src-1-in-the-hippocampus-of-male-mice
#3
Jikai Zhao, Chen Bian, Mengying Liu, Yangang Zhao, Tao Sun, Fangzhou Xing, Jiqiang Zhang
Hippocampal synaptic plasticity is the basis of spatial memory and cognition and is strongly regulated by both testicular androgens (testosterone, T) and hippocampal estrogens (17β-estradiol, E2) converted from T by aromatase, which is inhibited by letrozole (LET), but the contribution of each pathway to spatial memory and the associated mechanisms are unclear. In this study, we first used orchiectomy (ORX) and LET injection to investigate the effects of T and hippocampal E2 on spatial memory and hippocampal synaptic plasticity...
February 13, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29450726/c9orf72-associated-neurodegeneration-in-als-ftd-breaking-new-ground-in-ribosomal-rna-and-nucleolar-dysfunction
#4
REVIEW
Dustin Herrmann, Rosanna Parlato
Amyotrophic lateral sclerosis (ALS) and frontotemporal degeneration (FTD) are neurodegenerative diseases with distinct clinical appearance. However, both share as major genetic risk factor a C9orf72 locus intronic hexanucleotide expansion. The pathogenic pathways associated with the expansion-dependent neuronal toxicity are still poorly understood. Recent efforts to identify common threads of neuronal dysfunction have pointed towards deficits of ribosomal RNA (rRNA) biogenesis and loss of nucleolar integrity, a condition known as nucleolar stress that is an emerging shared feature among neurodegenerative diseases...
February 15, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29449881/a-copper-controlled-rna-interference-system-for-reversible-silencing-of-target-genes-in-trichoderma-reesei
#5
Lei Wang, Fanglin Zheng, Weixin Zhang, Yaohua Zhong, Guanjun Chen, Xiangfeng Meng, Weifeng Liu
Background: Trichoderma reesei is a primary lignocellulosic enzyme producer in industry. However, the mechanisms underlying cellulase synthesis as well as other physiological processes are insufficiently understood partly due to the sophisticated process for its genetic manipulation. Target gene knockdown by RNA interference (RNAi) is a powerful tool for genetic research and biotechnology in eukaryotes including filamentous fungi. Previously reported RNAi system in T. reesei was either uncontrollable or only applicable in certain nutrition state...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29449856/efficient-detection-of-long-dsrna-in-vitro-and-in-vivo-using-the-dsrna-binding-domain-from-fhv-b2-protein
#6
Baptiste Monsion, Marco Incarbone, Kamal Hleibieh, Vianney Poignavent, Ahmed Ghannam, Patrice Dunoyer, Laurent Daeffler, Jens Tilsner, Christophe Ritzenthaler
Double-stranded RNA (dsRNA) plays essential functions in many biological processes, including the activation of innate immune responses and RNA interference. dsRNA also represents the genetic entity of some viruses and is a hallmark of infections by positive-sense single-stranded RNA viruses. Methods for detecting dsRNA rely essentially on immunological approaches and their use is often limited to in vitro applications, although recent developments have allowed the visualization of dsRNA in vivo . Here, we report the sensitive and rapid detection of long dsRNA both in vitro and in vivo using the dsRNA binding domain of the B2 protein from Flock house virus...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29449840/c-abl-twist1-epigenetically-dysregulate-inflammatory-responses-during-mycobacterial-infection-by-co-regulating-bone-morphogenesis-protein-and-mir27a
#7
Kasturi Mahadik, Praveen Prakhar, R S Rajmani, Amit Singh, Kithiganahalli Narayanaswamy Balaji
Mycobacteria propelled modulation of host responses is of considerable interest in the face of emerging drug resistance. Although it is known that Abl tyrosine kinases affect entry and persistence of mycobacteria, mechanisms that couple c-Abl to proximal signaling pathways during immunity are poorly understood. Loss-of-function of c-Abl through Imatinib, in a mouse model of tuberculosis or RNA interference, identified bone morphogenesis protein (BMP) signaling as its cellular target. We demonstrate that c-Abl promotes mycobacterial survival through epigenetic modification brought about by KAT5-TWIST1 at Bmp loci...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29449668/rhinovirus-3c-protease-suppresses-apoptosis-and-triggers-caspase-independent-cell-death
#8
Mark Lötzerich, Pascal S Roulin, Karin Boucke, Robert Witte, Oleg Georgiev, Urs F Greber
Apoptosis and programmed necrosis (necroptosis) determine cell fate, and antagonize infection. Execution of these complementary death pathways involves the formation of receptor-interacting protein kinase 1 (RIPK1) containing complexes. RIPK1 binds to adaptor proteins, such as TRIF (Toll-IL-1 receptor-domain-containing-adaptor-inducing interferon-beta factor), FADD (Fas-associated-protein with death domain), NEMO (NF-κB regulatory subunit IKKγ), SQSTM1 (sequestosome 1/p62), or RIPK3 (receptor-interacting protein kinase 3), which are involved in RNA sensing, NF-κB signaling, autophagosome formation, apoptosis, and necroptosis...
February 15, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29449344/defeating-major-contaminants-in-fe-3-imac-phosphopeptide-enrichment
#9
Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer
Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with IMAC based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chloroform protein precipitation and enzymatic digestion using benzonase, which degrades all forms of DNA and RNA, prior to IMAC-column loading. This simple procedure resulted in a drastic increase of enrichment sensitivity, enabling the identification of around 17,000 unique phosphopeptides and 12,500 unambiguously localized phosphosites in human cell-lines from a single LC-MS/MS run, constituting a 50% increase when compared to the standard protocol...
February 15, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29448039/development-of-sirna-mediated-rna-interference-and-functional-analysis-of-novel-parasitic-nematode-specific-protein-of-setaria-digitata
#10
M B C L Somarathne, Y I N S Gunawardena, N V Chandrasekharan, R S Dassanayake
Despite the differences of the host, parasitic nematodes may share commonalities in their parasitizing genes. Setaria digitata novel protein (SDNP) is such an entity which is parasitic nematode-specific and having sequence similarities with those of W. bancrofti, B. malayi, Loa loa and Onchocerca volvulus. Post-transcriptional gene silencing by siRNA mediated RNA interference (RNAi) is a widely used technique in functional genomics. Though the technique has been used in several free-living, plant and animal parasitic nematodes, it has not yet been tried out for the filarial worm S...
February 12, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29447160/modeling-the-interactions-of-sense-and-antisense-period-transcripts-in-the-mammalian-circadian-clock-network
#11
Dorjsuren Battogtokh, Shihoko Kojima, John J Tyson
In recent years, it has become increasingly apparent that antisense transcription plays an important role in the regulation of gene expression. The circadian clock is no exception: an antisense transcript of the mammalian core-clock gene PERIOD2 (PER2), which we shall refer to as Per2AS RNA, oscillates with a circadian period and a nearly 12 h phase shift from the peak expression of Per2 mRNA. In this paper, we ask whether Per2AS plays a regulatory role in the mammalian circadian clock by studying in silico the potential effects of interactions between Per2 and Per2AS RNAs on circadian rhythms...
February 15, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29447077/identification-of-proteins-that-bind-to-the-neuroprotective-agent-neoechinulin-a
#12
Shinji Kamisuki, Natsumi Himeno, Yukine Tsurukawa, Tomoe Kusayanagi, Masahiro Takeno, Takashi Kamakura, Kouji Kuramochi, Fumio Sugawara
Neoechinulin A is an indole alkaloid with several biological activities. We previously reported that this compound protects neuronal PC12 cells from cytotoxicity induced by the peroxynitrite generator 3-morpholinosydnonimine (SIN-1), but the target proteins and precise mechanism of action of neoechinulin A were unclear. Here, we employed a phage display screen to identify proteins that bind directly with neoechinulin A. Our findings identified two proteins, chromogranin B and glutaredoxin 3, as candidate target binding partners for the alkaloid...
February 15, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29444946/karyopherin-alpha6-is-required-for-the-replication-of-porcine-reproductive-and-respiratory-syndrome-virus-and-zika-virus
#13
Liping Yang, Rong Wang, Shixing Yang, Zexu Ma, Shaoli Lin, Yuchen Nan, Qisheng Li, Qiyi Tang, Yan-Jin Zhang
Movement of macromolecules between the cytoplasm and the nucleus occurs through the nuclear pore complex (NPC). Karyopherins comprise a family of soluble transport factors facilitating nucleocytoplasmic translocation of proteins through the NPC. In this study, we discovered that karyopherin alpha6 (KPNA6, also known as importin alpha7) was required for the optimal replication of porcine reproductive and respiratory syndrome virus (PRRSV) and Zika virus (ZIKV), which are positive-sense, single-stranded RNA viruses replicating in the cytoplasm...
February 14, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29444937/maraviroc-is-associated-with-latent-hiv-1-reactivation-through-nf-%C3%AE%C2%BAb-activation-in-resting-cd4-t-cells-from-hiv-infected-individuals-on-suppressive-antiretroviral-therapy
#14
Nadia Madrid-Elena, María Laura García-Bermejo, Sergio Serrano-Villar, Alberto Díaz-de Santiago, Beatriz Sastre, Carolina Gutiérrez, Fernando Dronda, María Coronel Díaz, Ester Domínguez, María Rosa López-Huertas, Beatriz Hernández-Novoa, Santiago Moreno
Maraviroc is a CCR5 antagonist used in the treatment of HIV-1 infection. We and others have suggested that maraviroc could reactivate latent HIV-1. To test the latency reversing potential of maraviroc and the mechanisms involved, we performed a phase-II, single-center, open-label study in which maraviroc was administered for 10 days to 20 HIV-1-infected individuals on suppressive antiretroviral therapy (Eudra CT: 2012-003215-66). All patients completed full maraviroc dosing and follow up. The primary endpoint was to study whether maraviroc may reactivate HIV-1 latency, eliciting signalling pathways involved in the viral reactivation...
February 14, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29444517/acetyl-coa-synthetase-2-promotes-cell-migration-and-invasion-of-renal-cell-carcinoma-by-upregulating-lysosomal-associated-membrane-protein-1-expression
#15
Lv Yao, Xiaoqiang Guo, Yaoting Gui
BACKGROUND/AIMS: Reprogramming energy metabolism is an emerging hallmark of many cancers, and this alteration is especially evident in renal cell carcinomas (RCCs). However, few studies have been conducted on lipid metabolism. This study investigated the function and mechanism of lipid metabolism-related acetyl-CoA synthetase 2 (ACSS2) in RCC development, cell migration and invasion. METHODS: Quantitative real-time PCR (qRT-PCR) was used to determine the expression of ACSS2 in cancer tissue and adjacent tissue...
February 8, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29441628/a-serine-protease-homologue-bombyx-mori-scarface-induces-a-short-and-fat-body-shape-in-silkworm
#16
R-X Wang, X-L Tong, T-T Gai, C-L Li, L Qiao, H Hu, M-J Han, Z-H Xiang, C Lu, F-Y Dai
Body shape is one of the most prominent and basic characteristics of any organism. In insects, abundant variations in body shape can be observed both within and amongst species. However, the molecular mechanism underlying body shape fine-tuning is very complex and has been largely unknown until now. In the silkworm Bombyx mori, the tubby (tub) mutant has an abnormal short fat body shape and the abdomen of tub larvae expands to form a fusiform body shape. Morphological investigation revealed that the body length was shorter and the body width was wider than that of the Dazao strain...
February 14, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29440889/a-novel-therapeutic-strategy-for-cartilage-diseases-based-on-lipid-nanoparticle-rnai-delivery-system
#17
Shaowei Wang, Xiaochun Wei, Xiaojuan Sun, Chongwei Chen, Jingming Zhou, Ge Zhang, Heng Wu, Baosheng Guo, Lei Wei
Background: Cartilage degeneration affects millions of people but preventing its degeneration is a big challenge. Although RNA interference (RNAi) has been used in human trials via silencing specific genes, the cartilage RNAi has not been possible to date because the cartilage is an avascular and very dense tissue with very low permeability. Purpose: The objective of this study was to develop and validate a novel lipid nanoparticle (LNP)-siRNA delivery system that can prevent cartilage degeneration by knocking down specific genes...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29440594/the-compass-like-complex-promotes-flowering-and-panicle-branching-in-rice
#18
Pengfei Jiang, Shiliang Wang, Haiyang Jiang, Beijiu Cheng, Keqiang Wu, Yong Ding
Flowering time (heading date) and panicle branch number are important agronomic traits that determine yield in rice (Oryza sativa). The activation of flowering requires histone methylation, but the roles of tri-methylation of lysine 4 of histone 3 (H3K4me3) in modulating heading date and panicle development are unclear. Here, we showed that the COMPASS-like complex promotes flowering and panicle branching. The rice WD40 protein OsWDR5a interacts with the TRITHORAX-like protein OsTrx1/SDG723 to form the core components of the COMPASS-like complex...
February 12, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29438553/rnai-mediated-knockdown-of-vatpase-subunits-affects-survival-and-reproduction-of-bed-bugs-hemiptera-cimicidae
#19
Sanjay Basnet, Shripat T Kamble
The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) has resurged as one of the most troublesome household pests affecting people across the globe. Bed bug infestations have increased in recent years primarily due to the evolution of insecticide resistance and the insect's ability to hitchhike with travelers. vATPases are one of the most evolutionarily conserved holoenzymes in eukaryotes, which are mainly involved in proton transport across the plasma membranes and intracellular organelles. RNA interference (RNAi) has been developed as a promising tool for insect control...
February 9, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29438442/the-sirna-mediated-knockdown-of-glun3a-in-46c-derived-neural-stem-cells-affects-mrna-expression-levels-of-neural-genes-including-known-iglur-interactors
#20
Svenja Pachernegg, Sebastian Eilebrecht, Elke Eilebrecht, Hendrik Schöneborn, Sebastian Neumann, Arndt G Benecke, Michael Hollmann
For years, GluN3A was solely considered to be a dominant-negative modulator of NMDARs, since its incorporation into receptors alters hallmark features of conventional NMDARs composed of GluN1/GluN2 subunits. Only recently, increasing evidence has accumulated that GluN3A plays a more diversified role. It is considered to be critically involved in the maturation of glutamatergic synapses, and it might act as a molecular brake to prevent premature synaptic strengthening. Its expression pattern supports a putative role during neural development, since GluN3A is predominantly expressed in early pre- and postnatal stages...
2018: PloS One
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