keyword
MENU ▼
Read by QxMD icon Read
search

Rna induced silencing complex

keyword
https://www.readbyqxmd.com/read/29164518/reducing-production-of-fumonisin-mycotoxins-in-fusarium-verticillioides-by-rna-interference
#1
Eric T Johnson, Robert H Proctor, Christopher A Dunlap, Mark Busman
The fungus Fusarium verticillioides is a maize pathogen that can produce fumonisin mycotoxins in ears under certain environmental conditions. Because fumonisins pose health risks to humans and livestock, control strategies with minimal risk to the environment are needed to reduce fumonisin contamination. Host-induced gene silencing is a promising technique in which double-stranded RNA expressed in the plant host is absorbed by an invading fungus and down-regulates genes critical for pathogenicity or mycotoxin production in the fungus...
November 22, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/29133395/rna-stem-structure-governs-coupling-of-dicing-and-gene-silencing-in-rna-interference
#2
Hye Ran Koh, Amirhossein Ghanbariniaki, Sua Myong
PremicroRNAs (premiRNAs) possess secondary structures consisting of a loop and a stem with multiple mismatches. Despite the well-characterized RNAi pathway, how the structural features of premiRNA contribute to dicing and subsequent gene-silencing efficiency remains unclear. Using single-molecule FISH, we demonstrate that cytoplasmic mRNA, but not nuclear mRNA, is reduced during RNAi. The dicing rate and silencing efficiency both increase in a correlated manner as a function of the loop length. In contrast, mismatches in the stem drastically diminish the silencing efficiency without impacting the dicing rate...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29132374/yeast-interfering-rna-larvicides-targeting-neural-genes-induce-high-rates-of-anopheles-larval-mortality
#3
Keshava Mysore, Limb K Hapairai, Longhua Sun, Elizabeth I Harper, Yingying Chen, Kathleen K Eggleson, Jacob S Realey, Nicholas D Scheel, David W Severson, Na Wei, Molly Duman-Scheel
BACKGROUND: Although larviciding can reduce the number of outdoor biting malaria vector mosquitoes, which may help to prevent residual malaria transmission, the current larvicide repertoire is faced with great challenges to sustainability. The identification of new effective, economical, and biorational larvicides could facilitate maintenance and expansion of the practice of larviciding in integrated malaria vector mosquito control programmes. Interfering RNA molecules represent a novel class of larvicides with untapped potential for sustainable mosquito control...
November 13, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29115409/mechanism-of-long-non-coding-rna-malat1-in-lipopolysaccharide-induced-acute-kidney-injury-is-mediated-by-the-mir-146a-nf-%C3%AE%C2%BAb-signaling-pathway
#4
Ying Ding, Feng Guo, Tao Zhu, Jun Li, Danyan Gu, Weiliang Jiang, Yuying Lu, Daoyang Zhou
The present study aimed to examine the expression and function of the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/microRNA (miR)-146a/nuclear factor (NF)-κB axis in lipopolysaccharide (LPS)-induced acute kidney injury (AKI). The mRNA levels of MALAT1 and miR-146a in AKI tissues and cells were detected using reverse transcription-quantitative polymerase chain reaction analysis. The NF-κB pathway proteins and cell viability were assessed using western blot analysis and the MTT method, respectively...
November 2, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29075278/hop-sti1-a-two-faced-cochaperone-involved-in-pattern-recognition-receptor-maturation-and-viral-infection
#5
Christian E Lamm, Max E Kraner, Jörg Hofmann, Frederik Börnke, Hans-Peter Mock, Uwe Sonnewald
Perception of pathogens by host pattern recognition receptors (PRRs) or R proteins is a prerequisite to promote successful immune responses. The Hsp70/Hsp90 organizing protein Hop/Sti1, a multifunctional cochaperone, has been implicated in the maturation of a receptor-like kinase (RLK) necessary for chitin sensing. However, it remains unknown whether Hop/Sti1 is generally participating in PRR genesis. Using RNA-interference (RNAi), we silenced Hop/Sti1 expression in Nicotiana tabacum to gain further insight into the role of the cochaperone in plant defense responses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29070679/the-e3-ubiquitin-ligase-scf-fbxl14-complex-stimulates-neuronal-differentiation-by-targeting-the-notch-signaling-factor-hes1-for-proteolysis
#6
Fangfang Chen, Chunxiao Zhang, Haonan Wu, Yue Ma, Xiaomin Luo, Xinqi Gong, Fan Jiang, Yaoting Gui, Hui Zhang, Fei Lu
Molecular oscillators are important cellular regulators of, for example, circadian clocks, oscillations of immune regulators, and short-period (ultradian) rhythms during embryonic development. The Notch signaling factor hairy and enhancer of split 1 (HES1) is a well-known repressor of proneural genes, and HES1 ultradian oscillation is essential for keeping cells in an efficiently proliferating progenitor state. HES1 oscillation is driven by both transcriptional self-repression and ubiquitin-dependent proteolysis...
October 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29070517/host-induced-gene-silencing-of-the-mapkk-gene-psfuz7-confers-stable-resistance-to-wheat-stripe-rust
#7
Xiaoguo Zhu, Tuo Qi, Qian Yang, Fuxin He, Chenglong Tan, Wei Ma, Ralf Thomas Voegele, Zhensheng Kang, Jun Guo
RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and to control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus Puccinia striiformis f. sp. tritici (Pst), remained largely under explored, so far. To address this issue, we generated transgenic wheat lines expressing double-stranded RNA targeting PsFUZ7 transcripts of Pst. We analyzed expression of PsFUZ7 and related genes, and resistance traits of the transgenic wheat lines...
October 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29061908/the-rna-editing-factor-slorrm4-is-required-for-normal-fruit-ripening-in-tomato
#8
Yongfang Yang, Guoning Zhu, Rui Li, Shijie Yan, Daqi Fu, Benzhong Zhu, Huiqin Tian, Yunbo Luo, Hongliang Zhu
RNA editing plays a key post-transcriptional role in gene expression. Existing studies on cytidine (C)-to-uridine (U) RNA editing in plants have focused on maize, rice, and Arabidopsis. However, the importance and regulation of RNA editing in several critical agronomic processes are not well understood, a notable example of which is fruit ripening. Here, we analyzed the expression profile of 33 RNA editing factors and identified 11 putative tomato fruit ripening-related factors. A rapid virus-induced gene silencing (VIGS) assay indicated that organelle RNA recognition motif-containing protein SlORRM4 affected tomato fruit ripening...
October 23, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29056324/the-dynamics-of-mrna-turnover-revealed-by-single-molecule-imaging-in-single-cells
#9
Ivana Horvathova, Franka Voigt, Anna V Kotrys, Yinxiu Zhan, Caroline G Artus-Revel, Jan Eglinger, Michael B Stadler, Luca Giorgetti, Jeffrey A Chao
RNA degradation plays a fundamental role in regulating gene expression. In order to characterize the spatiotemporal dynamics of RNA turnover in single cells, we developed a fluorescent biosensor based on dual-color, single-molecule RNA imaging that allows intact transcripts to be distinguished from stabilized degradation intermediates. Using this method, we measured mRNA decay in single cells and found that individual degradation events occur independently within the cytosol and are not enriched within processing bodies...
November 2, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29049866/melanoma-sponge-on-pigmentation-gene-to-reduce-tumour-suppressive-micrornas
#10
Peter Dietrich, Anja Katrin Bosserhoff
MicroRNAs (miRs) were originally described as non-coding RNA molecules that mediate the inhibition of translation of their target genes by binding to distinctive, now called canonical, MicroRNA Response Elements (MREs). After binding to MREs, which are usually located near the 3'UTR of their target mRNA, a complex protein machinery (RNA-induced silencing complex, RISC) mediates mRNA decay and/or the inhibition of translation initiation (Mione and Bosserhoff, 2015). However, the diversity of microRNA regulation and function has greatly expanded over the last several years, and more non-canonical functions of miRs are emerging...
October 19, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/29040648/molecular-basis-for-asymmetry-sensing-of-sirnas-by-the-drosophila-loqs-pd-dcr-2-complex-in-rna-interference
#11
Jan-Niklas Tants, Stephanie Fesser, Thomas Kern, Ralf Stehle, Arie Geerlof, Christoph Wunderlich, Michael Juen, Christoph Hartlmüller, Romy Böttcher, Stefan Kunzelmann, Oliver Lange, Christoph Kreutz, Klaus Förstemann, Michael Sattler
RNA interference defends against RNA viruses and retro-elements within an organism's genome. It is triggered by duplex siRNAs, of which one strand is selected to confer sequence-specificity to the RNA induced silencing complex (RISC). In Drosophila, Dicer-2 (Dcr-2) and the double-stranded RNA binding domain (dsRBD) protein R2D2 form the RISC loading complex (RLC) and select one strand of exogenous siRNAs according to the relative thermodynamic stability of base-pairing at either end. Through genome editing we demonstrate that Loqs-PD, the Drosophila homolog of human TAR RNA binding protein (TRBP) and a paralog of R2D2, forms an alternative RLC with Dcr-2 that is required for strand choice of endogenous siRNAs in S2 cells...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29030847/single-molecule-analysis-for-risc-assembly-and-target-cleavage
#12
Hiroshi M Sasaki, Hisashi Tadakuma, Yukihide Tomari
RNA-induced silencing complex (RISC) is a small RNA-protein complex that mediates silencing of complementary target RNAs. Biochemistry has been successfully used to characterize the molecular mechanism of RISC assembly and function for nearly two decades. However, further dissection of intermediate states during the reactions has been warranted to fill in the gaps in our understanding of RNA silencing mechanisms. Single-molecule analysis with total internal reflection fluorescence (TIRF) microscopy is a powerful imaging-based approach to interrogate complex formation and dynamics at the individual molecule level with high sensitivity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29030846/reconstitution-of-rna-interference-machinery
#13
Shintaro Iwasaki, Yukihide Tomari
Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), silence protein expression from target mRNAs bearing their complementary sequences, via the formation of the effector complex called RNA-induced silencing complex (RISC). Although the mechanism of RISC assembly has been studied for nearly two decades, the detailed mechanism has still remained unclear in part due to the lack of a pure reconstitution system. Recently, we identified all the core proteins necessary for RISC assembly in flies and successfully recapitulated the assembly of catalytically active RISC with eight recombinant proteins...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29030842/microrna-detection-by-whole-mount-in-situ-hybridization-in-c-elegans
#14
Yoshiki Andachi, Yuji Kohara
MicroRNAs (miRNAs) loaded on argonaute proteins guide RNA-induced silencing complexes to target mRNAs. An excellent method to decipher the spatiotemporal expression patterns of miRNAs is whole-mount in situ hybridization (WISH), which has been successfully used in vertebrate embryos but still remains unavailable for many animal species. Here, we describe a WISH method for miRNA detection in Caenorhabditis elegans at both embryonic and post-embryonic stages. Strategies devised for detection include fixation of animals with carbodiimide at a high temperature and subsequent partial digestion of the fixed animals with an extremely high concentration of proteinase...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29030839/quantification-of-mirnas-co-immunoprecipitated-with-argonaute-proteins-using-sybr-green-based-qrt-pcr
#15
Hong-Duc Phan, Junan Li, Ming Poi, Kotaro Nakanishi
MicroRNAs (miRNAs) are small non-coding RNAs that trigger post-transcriptional gene silencing. These RNAs need to be associated with the Argonaute proteins to be functional. This assembly begins with loading of a miRNA duplex, followed by the ejection of one of the strands (passenger). The remaining strand (guide) together with the Argonaute protein forms a ribonucleoprotein effector complex (the RNA-induced silencing complex, RISC). Mutation on the Argonaute protein, if affecting either step of the RISC assembly, impacts the function of miRNAs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29028222/role-of-rbp2-induced-er-and-igf1r-erbb-signaling-in-tamoxifen-resistance-in-breast-cancer
#16
Hee-Joo Choi, Hyeong-Seok Joo, Hee-Young Won, Kyueng-Whan Min, Hyung-Yong Kim, Taekwon Son, Young-Ha Oh, Jeong-Yeon Lee, Gu Kong
Background: Despite the benefit of endocrine therapy, acquired resistance during or after treatment still remains a major challenge in estrogen receptor (ER)-positive breast cancer. We investigated the potential role of histone demethylase retinoblastoma-binding protein 2 (RBP2) in endocrine therapy resistance of breast cancer. Methods: Survival of breast cancer patients according to RBP2 expression was analyzed in three different breast cancer cohorts including METABRIC (n = 1980) and KM plotter (n = 1764)...
April 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28987883/galnac-bio-functionalization-of-nanoparticles-assembled-by-electrostatic-interactions-improves-sirna-targeting-to-the-liver
#17
Efrat Korin, Tzlil Bejerano, Smadar Cohen
RNA interference (RNAi) has the potential to reversibly silence any gene with high efficiency and specificity. To fulfill the clinical potential of RNAi, delivery vehicles are required to transport the short interfering RNA (siRNA) to the site of action in the cells of target tissues. Here, we describe the features of novel liver-targeted siRNA nanoparticles (NPs), co-assembled due to the complexation of alginate sulfate (AlgS) with siRNA, mediated by calcium ions bridges (AlgS-Ca(2+)-siRNA NPs) and then bioconjugation of a targeting ligand onto the AlgS upon the NP surface...
October 4, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28977639/biochemical-and-single-molecule-analyses-of-the-rna-silencing-suppressing-activity-of-crpv-1a
#18
Mariko Watanabe, Hiro-Oki Iwakawa, Hisashi Tadakuma, Yukihide Tomari
Viruses often encode viral silencing suppressors (VSSs) to counteract the hosts' RNA silencing activity. The cricket paralysis virus 1A protein (CrPV-1A) is a unique VSS that binds to a specific Argonaute protein (Ago)-the core of the RNA-induced silencing complex (RISC)-in insects to suppress its target cleavage reaction. However, the precise molecular mechanism of CrPV-1A action remains unclear. Here we utilized biochemical and single-molecule imaging approaches to analyze the effect of CrPV-1A during target recognition and cleavage by Drosophila Ago2-RISC...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28973449/expression-of-short-hairpin-rnas-using-the-compact-architecture-of-retroviral-microrna-genes
#19
James M Burke, Rodney P Kincaid, Francesca Aloisio, Nicole Welch, Christopher S Sullivan
Short hairpin RNAs (shRNAs) are effective in generating stable repression of gene expression. RNA polymerase III (RNAP III) type III promoters (U6 or H1) are typically used to drive shRNA expression. While useful for some knockdown applications, the robust expression of U6/H1-driven shRNAs can induce toxicity and generate heterogeneous small RNAs with undesirable off-target effects. Additionally, typical U6/H1 promoters encompass the majority of the ∼270 base pairs (bp) of vector space required for shRNA expression...
September 29, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28971854/in-vitro-reconstitution-of-chaperone-mediated-human-risc-assembly
#20
Ken Naruse, Eriko Matsuura-Suzuki, Mariko Watanabe, Shintaro Iwasaki, Yukihide Tomari
To silence target mRNAs, small RNAs and Argonaute (Ago) proteins need to be assembled into RNA-induced silencing complexes (RISCs). Although the assembly of Drosophila melanogaster RISC was recently reconstituted by Ago2, the Dicer-2/R2D2 heterodimer, and 5 chaperone proteins, the absence of a reconstitution system for mammalian RISC assembly has posed analytical challenges. Here we describe reconstitution of human RISC assembly using Ago2 and 5 recombinant chaperone proteins: Hsp90β, Hsc70, Hop, Dnaja2, and p23...
October 2, 2017: RNA
keyword
keyword
67221
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"