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https://www.readbyqxmd.com/read/29218433/s8orf2-protein-of-infectious-salmon-anaemia-virus-is-a-rna-silencing-suppressor-and-interacts-with-salmon-salar-mov10-ssmov10-of-the-host-rnai-machinery
#1
Vandana Thukral, Bhavna Varshney, Rimatulhana B Ramly, Sanket S Ponia, Sumona Karjee Mishra, Christel M Olsen, Akhil C Banerjea, Sunil K Mukherjee, Rana Zaidi, Espen Rimstad, Sunil K Lal
The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi...
December 7, 2017: Virus Genes
https://www.readbyqxmd.com/read/29205436/validation-of-the-pain-resilience-scale-in-chinese-speaking-patients-with-tmd-pain
#2
Songlin He, Jinhua Wang, Ping Ji
To validate of the Pain Resilience Scale (PRS) for use in Chinese patients with temporomandibular disorders (TMD) pain. According to international guidelines, the original PRS was first translated and cross-culturally adapted to formulate the Chinese version of PRS (PRS-C). A total of 152 patients with TMD pain were recruited to complete series of questionnaires. Reliability of the PRS-C was investigated using internal consistency and test-retest reliability. Validity of the PRS-C was calculated using cross-cultural validity and convergent validity...
December 4, 2017: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/29203416/quantitative-contributions-of-processes-by-which-polyanion-drugs-reduce-intracellular-bioavailability-and-transfection-efficiency-of-cationic-sirna-lipoplex
#3
Pharavee Jaiprasart, Bertrand Z Yeung, Ze Lu, M Guillaume Wientjes, Minjian Cui, Chien-Ming Hsieh, Sukyung Woo, Jessie L-S Au
RNA Interference (RNAi) is a potentially useful tool to correct the detrimental effects of faulty genes; several RNAi are undergoing clinical evaluation in various diseases. The present study identified the relative contributions of three mechanisms by which polyanion drugs reduced the gene silencing activity of Lipoplex, a complex of small interfering RNA (siRNA) and cationic liposomes. The study used a siRNA against the chemoresistance gene survivin and two model polyanion drugs (suramin, heparin). Products of Lipoplex destabilization were separated, identified, and/or quantified using ultrafiltration, gel electrophoresis, and RT-qPCR (quantitative reverse transcription polymerase chain reaction)...
December 1, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29194361/social-networks-engagement-and-resilience-in-university-students
#4
Elena Fernández-Martínez, Elena Andina-Díaz, Rosario Fernández-Peña, Rosa García-López, Iván Fulgueiras-Carril, Cristina Liébana-Presa
Analysis of social networks may be a useful tool for understanding the relationship between resilience and engagement, and this could be applied to educational methodologies, not only to improve academic performance, but also to create emotionally sustainable networks. This descriptive study was carried out on 134 university students. We collected the network structural variables, degree of resilience (CD-RISC 10), and engagement (UWES-S). The computer programs used were excel, UCINET for network analysis, and SPSS for statistical analysis...
December 1, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29170131/negative-regulation-of-lncrna-gas5-by-mir-196a-inhibits-esophageal-squamous-cell-carcinoma-growth
#5
Kai Wang, Juan Li, Gang Xiong, Gang He, Xingying Guan, Kang Yang, Yun Bai
MiR-196a could play important roles in carcinogenesis by targeting many protein coding genes. However, little is known about whether miR-196a can target any long non-coding RNAs (lncRNAs). In the present study, we screen lncRNAs which are regulated by miRNA-196a in human esophageal squamous cell carcinoma (ESCC). We found that miR-196a could suppress the expression of lncRNA growth arrest-specific 5(GAS5). GAS5 is frequently down-regulated in 86 paired human ESCC tissues. Importantly, there was lower GAS5 expression in the late stage of ESCC patients...
November 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29125907/risk-of-type-2-diabetes-mellitus-development-in-the-native-population-of-low-and-high-altitude-regions-of-kyrgyzstan-finnish-diabetes-risc-score-questionnaire-results
#6
Marina S Moldobaeva, Anastasiya V Vinogradova, Marina K Esenamanova
Moldobaeva, Marina S., Anastasiya V. Vinogradova, and Marina K. Esenamanova. Risk of type 2 diabetes mellitus development in the native population of low- and high-altitude regions of Kyrgyzstan: Finnish Diabetes Risc Score questionnaire results. High Alt Med Biol. 00:000-000, 2017. OBJECTIVE: The number of patients with diabetes is steadily growing, but likely only half of all cases are ever identified. The Kyrgyz, native inhabitants of Central Asia, live in the mountainous area and have a particular lifestyle and nutrition...
November 10, 2017: High Altitude Medicine & Biology
https://www.readbyqxmd.com/read/29116825/spanish-validation-of-the-10-item-connor-davidson-resilience-scale-cd-risc-10-with-non-professional-caregivers
#7
Vanessa Blanco, María Adelina Guisande, María Teresa Sánchez, Patricia Otero, Fernando L Vázquez
OBJECTIVES: Despite the importance of resilience in populations under stress, and the fact that the 10-item version Connor-Davidson Resilience Scale (CD-RISC 10) is the shortest instrument for reliable and valid evaluation of resilience, there are no data on their psychometric properties in non-professional caregivers. The aim of this study was to analyze the psychometric properties and factorial structure of the spanish version of the CD-RISC 10 in non-professional caregivers. METHOD: Independently trained assessors evaluated resilience, self-esteem, social support, emotional distress and depression in a sample of 294 caregivers (89...
November 8, 2017: Aging & Mental Health
https://www.readbyqxmd.com/read/29096679/epidemiological-comparison-between-the-navarra-major-trauma-registry-and-the-german-trauma-registry-tr-dgu%C3%A2
#8
B Ali Ali, R Lefering, M Fortun Moral, T Belzunegui Otano
BACKGROUND: International benchmarking can help identify trauma system performance issues and determine the extent to which other countries also experience these. When problems are identified, countries can look to high performers for insight into possible responses. The objective of this study was to compare the treatment and outcome of severely injured patients in Germany and Navarra, Spain. METHODS: Data collected, from 2010 to 2013, in the Navarra Major Trauma Registry (NMTR) and the TraumaRegister DGU® (TR-DGU) were compared...
November 2, 2017: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/29078203/using-the-kannada-version-of-the-connor-davidson-resilience-scale-to-assess-resilience-and-its-relationship-with-psychological-distress-among-adolescent-girls-in-bangalore-india
#9
K P Fasli Sidheek, Veena A Satyanarayana, H R Sowmya, Prabha S Chandra
A widely used and accepted scale for assessing resilience is the Connor-Davidson Resilience Scale (CD-RISC). The aim of the present study was to establish the psychometric properties of the Kannada version of the scale and assess the relationship between resilience and psychological distress in a sample of adolescent girls living in low-income settings. Data was obtained from a sample of 606 adolescent girls studying in a college meant for women from a socio-economically disadvantaged setting. The CD- RISC (25 item) was used to assess resilience and Kessler Psychological Distress Scale (K10) was used to assess psychological distress...
October 18, 2017: Asian Journal of Psychiatry
https://www.readbyqxmd.com/read/29077785/impact-of-contemporary-patterns-of-chemotherapy-utilization-on-survival-in-patients-with-advanced-cancer-of-the-urinary-tract-a-retrospective-international-study-of-invasive-advanced-cancer-of-the-urothelium-risc
#10
A Bamias, K Tzannis, L C Harshman, S J Crabb, Y-N Wong, S Kumar Pal, U De Giorgi, S Ladoire, N Agarwal, E Y Yu, G Niegisch, A Necchi, C N Sternberg, S Srinivas, A Alva, U Vaishampayan, L Cerbone, M Liontos, J Rosenberg, T Powles, J Belmunt, M D Galsky
BackgroundCisplatin-based combination chemotherapy is the standard treatment for advanced urinary tract cancer (aUTC) but 50% of patients are ineligible for cisplatin according to recently published criteria. We used a multinational database to study patterns of chemotherapy utilization in patients with aUTC and determine their impact on survival.Patients and MethodsThis was a retrospective study of patients with: UTC (bladder, renal pelvis, ureter or urethra); advanced disease (stages T4b and/or N+ and/or M+); urothelial, squamous or adenocarcinoma histology...
October 25, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29073743/eye-movement-desensitization-and-reprocessing-to-facilitate-posttraumatic-growth-a-prospective-clinical-pilot-study-on-ferry-disaster-survivors
#11
Sang Won Jeon, Changsu Han, Joonho Choi, Young-Hoon Ko, Ho-Kyoung Yoon, Yong-Ku Kim
Objective: The purpose of this study was to investigate the therapeutic effects of eye movement desensitization and reprocessing (EMDR) on post-traumatic growth (PTG). Methods: This study was conducted using a sample of ten survivors of a large-scale maritime disaster that occurred in the Yellow Sea, South Korea, in April 2014. A total of eight EMDR sessions were administered by a psychiatrist at two-week intervals over a period of five months, starting two or three months after the accident...
November 30, 2017: Clinical Psychopharmacology and Neuroscience: the Official Scientific Journal of the Korean College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/29049866/melanoma-sponge-on-pigmentation-gene-to-reduce-tumour-suppressive-micrornas
#12
Peter Dietrich, Anja Katrin Bosserhoff
MicroRNAs (miRs) were originally described as non-coding RNA molecules that mediate the inhibition of translation of their target genes by binding to distinctive, now called canonical, MicroRNA Response Elements (MREs). After binding to MREs, which are usually located near the 3'UTR of their target mRNA, a complex protein machinery (RNA-induced silencing complex, RISC) mediates mRNA decay and/or the inhibition of translation initiation (Mione and Bosserhoff, 2015). However, the diversity of microRNA regulation and function has greatly expanded over the last several years, and more non-canonical functions of miRs are emerging...
October 19, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/29040648/molecular-basis-for-asymmetry-sensing-of-sirnas-by-the-drosophila-loqs-pd-dcr-2-complex-in-rna-interference
#13
Jan-Niklas Tants, Stephanie Fesser, Thomas Kern, Ralf Stehle, Arie Geerlof, Christoph Wunderlich, Michael Juen, Christoph Hartlmüller, Romy Böttcher, Stefan Kunzelmann, Oliver Lange, Christoph Kreutz, Klaus Förstemann, Michael Sattler
RNA interference defends against RNA viruses and retro-elements within an organism's genome. It is triggered by duplex siRNAs, of which one strand is selected to confer sequence-specificity to the RNA induced silencing complex (RISC). In Drosophila, Dicer-2 (Dcr-2) and the double-stranded RNA binding domain (dsRBD) protein R2D2 form the RISC loading complex (RLC) and select one strand of exogenous siRNAs according to the relative thermodynamic stability of base-pairing at either end. Through genome editing we demonstrate that Loqs-PD, the Drosophila homolog of human TAR RNA binding protein (TRBP) and a paralog of R2D2, forms an alternative RLC with Dcr-2 that is required for strand choice of endogenous siRNAs in S2 cells...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29030847/single-molecule-analysis-for-risc-assembly-and-target-cleavage
#14
Hiroshi M Sasaki, Hisashi Tadakuma, Yukihide Tomari
RNA-induced silencing complex (RISC) is a small RNA-protein complex that mediates silencing of complementary target RNAs. Biochemistry has been successfully used to characterize the molecular mechanism of RISC assembly and function for nearly two decades. However, further dissection of intermediate states during the reactions has been warranted to fill in the gaps in our understanding of RNA silencing mechanisms. Single-molecule analysis with total internal reflection fluorescence (TIRF) microscopy is a powerful imaging-based approach to interrogate complex formation and dynamics at the individual molecule level with high sensitivity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29030846/reconstitution-of-rna-interference-machinery
#15
Shintaro Iwasaki, Yukihide Tomari
Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), silence protein expression from target mRNAs bearing their complementary sequences, via the formation of the effector complex called RNA-induced silencing complex (RISC). Although the mechanism of RISC assembly has been studied for nearly two decades, the detailed mechanism has still remained unclear in part due to the lack of a pure reconstitution system. Recently, we identified all the core proteins necessary for RISC assembly in flies and successfully recapitulated the assembly of catalytically active RISC with eight recombinant proteins...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29030839/quantification-of-mirnas-co-immunoprecipitated-with-argonaute-proteins-using-sybr-green-based-qrt-pcr
#16
Hong-Duc Phan, Junan Li, Ming Poi, Kotaro Nakanishi
MicroRNAs (miRNAs) are small non-coding RNAs that trigger post-transcriptional gene silencing. These RNAs need to be associated with the Argonaute proteins to be functional. This assembly begins with loading of a miRNA duplex, followed by the ejection of one of the strands (passenger). The remaining strand (guide) together with the Argonaute protein forms a ribonucleoprotein effector complex (the RNA-induced silencing complex, RISC). Mutation on the Argonaute protein, if affecting either step of the RISC assembly, impacts the function of miRNAs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29029897/worldwide-trends-in-body-mass-index-underweight-overweight-and-obesity-from-1975-to-2016-a-pooled-analysis-of-2416-population-based-measurement-studies-in-128%C3%A2-9-million-children-adolescents-and-adults
#17
(no author information available yet)
BACKGROUND: Underweight, overweight, and obesity in childhood and adolescence are associated with adverse health consequences throughout the life-course. Our aim was to estimate worldwide trends in mean body-mass index (BMI) and a comprehensive set of BMI categories that cover underweight to obesity in children and adolescents, and to compare trends with those of adults. METHODS: We pooled 2416 population-based studies with measurements of height and weight on 128·9 million participants aged 5 years and older, including 31·5 million aged 5-19 years...
October 10, 2017: Lancet
https://www.readbyqxmd.com/read/29017520/the-nuclear-receptor-er%C3%AE-engages-ago2-in-regulation-of-gene-transcription-rna-splicing-and-risc-loading
#18
Roberta Tarallo, Giorgio Giurato, Giuseppina Bruno, Maria Ravo, Francesca Rizzo, Annamaria Salvati, Luca Ricciardi, Giovanna Marchese, Angela Cordella, Teresa Rocco, Valerio Gigantino, Biancamaria Pierri, Giovanni Cimmino, Luciano Milanesi, Concetta Ambrosino, Tuula A Nyman, Giovanni Nassa, Alessandro Weisz
BACKGROUND: The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts a pivotal role in microRNA maturation and activity and can modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. Estrogen receptor beta (ERβ) is endowed with oncosuppressive activities, antagonizing hormone-induced carcinogenesis and inhibiting growth and oncogenic functions in luminal-like breast cancers (BCs), where its expression correlates with a better prognosis of the disease...
October 6, 2017: Genome Biology
https://www.readbyqxmd.com/read/28986804/rnai-mediated-knockdown-of-protein-expression
#19
Volker Baumann, Cornelia Lorenzer, Michael Thell, Anna-Maria Winkler, Johannes Winkler
RNA interference is an essential method for studying genomic functions of single genes by loss-of-function experiments. Short interfering siRNAs are efficiently transfected into cultured cells to enable RISC-mediated mRNA cleavage and inhibition of translation in a sequence-specific manner. RNAi enables knockdown of single genes and screening for specific cellular processes or outcomes. In this chapter, we describe a detailed universal protocol for lipoplex-mediated siRNA transfection for cell cultures and cell lysis for subsequent RNA or protein analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28981809/impact-of-enhanced-metabolic-stability-on-pharmacokinetics-and-pharmacodynamics-of-galnac-sirna-conjugates
#20
Jayaprakash K Nair, Husain Attarwala, Alfica Sehgal, Qianfan Wang, Krishna Aluri, Xuemei Zhang, Minggeng Gao, Ju Liu, Ramesh Indrakanti, Sally Schofield, Philip Kretschmer, Christopher R Brown, Swati Gupta, Jennifer L S Willoughby, Julie A Boshar, Vasant Jadhav, Klaus Charisse, Tracy Zimmermann, Kevin Fitzgerald, Muthiah Manoharan, Kallanthottathil G Rajeev, Akin Akinc, Renta Hutabarat, Martin A Maier
Covalent attachment of a synthetic triantennary N-acetylagalactosamine (GalNAc) ligand to chemically modified siRNA has enabled asialoglycoprotein (ASGPR)-mediated targeted delivery of therapeutically active siRNAs to hepatocytes in vivo. This approach has become transformative for the delivery of RNAi therapeutics as well as other classes of investigational oligonucleotide therapeutics to the liver. For efficient functional delivery of intact drug into the desired subcellular compartment, however, it is critical that the nucleic acids are stabilized against nucleolytic degradation...
September 15, 2017: Nucleic Acids Research
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