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Andrea Necchi, Guru Sonpavde, Salvatore Lo Vullo, Daniele Giardiello, Aristotelis Bamias, Simon J Crabb, Lauren C Harshman, Joaquim Bellmunt, Ugo De Giorgi, Cora N Sternberg, Linda Cerbone, Sylvain Ladoire, Yu-Ning Wong, Evan Y Yu, Simon Chowdhury, Gunter Niegisch, Sandy Srinivas, Ulka N Vaishampayan, Sumanta K Pal, Neeraj Agarwal, Ajjai Alva, Jack Baniel, Ali-Reza Golshayan, Rafael Morales-Barrera, Daniel W Bowles, Matthew I Milowsky, Christine Theodore, Dominik R Berthold, Gedske Daugaard, Srikala S Sridhar, Thomas Powles, Jonathan E Rosenberg, Matthew D Galsky, Luigi Mariani
BACKGROUND: The available prognostic models for overall survival (OS) in patients with metastatic urothelial carcinoma (UC) have been derived from clinical trial populations of cisplatin-treated patients. OBJECTIVE: To develop a new model based on real-world patients. DESIGN, SETTING, AND PARTICIPANTS: Individual patient-level data from 29 centers were collected, including metastatic UC and first-line cisplatin- or carboplatin-based chemotherapy administered between January 2006 and January 2011...
October 8, 2016: European Urology
Noriko Saito-Tarashima, Hirotaka Kira, Tomoya Wada, Kazuya Miki, Shiho Ide, Naoshi Yamazaki, Akira Matsuda, Noriaki Minakawa
Elucidation of dynamic interactions between RNA and proteins is essential for understanding the biological processes regulated by RNA, such as RNA interference (RNAi). In this study, the logical chemical probes, comprising 7-bromo-7-deazaadenosine (Br(7)C(7)A) and 3-bromo-3-deazaadenosine (Br(3)C(3)A), to investigate small interfering RNA (siRNA)-RNAi related protein interactions, were developed. The bromo substituents of Br(7)C(7)A and Br(3)C(3)A are expected to be located in the major and the minor grooves, respectively, and to act as a steric hindrance in each groove when these chemical probes are incorporated into siRNAs...
September 28, 2016: Organic & Biomolecular Chemistry
Diane Chamberlain, Allison Williams, David Stanley, Peter Mellor, Wendy Cross, Lesley Siegloff
BACKGROUND: Nursing students will graduate into stressful workplace environments and resilience is an essential acquired ability for surviving the workplace. Few studies have explored the relationship between resilience and the degree of innate dispositional mindfulness, compassion, compassion fatigue and burnout in nursing students, including those who find themselves in the position of needing to work in addition to their academic responsibilities. AIM: This paper investigates the predictors of resilience, including dispositional mindfulness and employment status of third year nursing students from three Australian universities...
October 2016: Nurs Open
Aleksandra Poterala, Joanna Rzeszowska-Wolny
Ago proteins are members of the highly specialized and conserved Argonaute family, primarily responsible for regulation of gene expression. As a part of RNA-induced silencing complexes (RISCs) Ago proteins are responsible for binding a short RNA and cleavage/inhibition of translation of target mRNAs. Phosphorylation may work as the switch between those two functions, but the role of magnesium ion concentration is also taken into consideration. Recent reports indicate that Ago proteins can interact with an mRNA and cause inhibition of translation without the participation of a short RNA...
September 28, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
Matthew A Lafreniere, Megan H Powdrill, Ragunath Singaravelu, John Paul Pezacki
Many viruses, including the hepatitis C virus (HCV), are dependent on the host RNA silencing pathway for replication. In this study, we screened small molecule probes, previously reported to disrupt loading of the RNA-induced silencing complex (RISC), including 6-hydroxydopamine (6-OHDA), suramin (SUR), and aurintricarboxylic acid (ATA), to examine their effects on viral replication. We found that 6-OHDA inhibited HCV replication; however, 6-OHDA was a less potent inhibitor of RISC than either SUR or ATA. By generating a novel chemical probe (6-OHDA-yne), we determined that 6-OHDA covalently modifies host and virus proteins...
October 13, 2016: ACS Infectious Diseases
Vladimir S Trubetskoy, Jacob B Griffin, Anthony L Nicholas, Eric M Nord, Zhao Xu, Ryan M Peterson, Christine I Wooddell, David B Rozema, Darren H Wakefield, David L Lewis, Steven B Kanner
The RNA interference (RNAi)-based therapeutic ARC-520 for chronic hepatitis B virus (HBV) infection consists of a melittin-derived peptide conjugated to N-acetylgalactosamine for hepatocyte targeting and endosomal escape, and cholesterol-conjugated RNAi triggers, which together result in HBV gene silencing. To characterize the kinetics of RNAi trigger delivery and 5'-phosphorylation of guide strands correlating with gene knockdown, we employed a peptide-nucleic acid (PNA) hybridization assay. A fluorescent sense strand PNA probe binding to RNAi duplex guide strands was coupled with anion exchange high performance liquid chromatography to quantitate guide strands and metabolites...
September 19, 2016: Nucleic Acids Research
Jiahua Hu, Qun Zhang, Yi Luo
Among various nonradiative photophysical processes related to energy migration in singlet-triplet coupled molecular systems, unlike such processes as internal conversion, intersystem crossing (ISC), and intramolecular vibrational relaxation that have been extensively addressed, the reverse ISC (rISC) is a unique one that apparently lacks sufficient interrogation probably owing to its intrinsically elusive nature. In particular, it still remains a nontrivial task to quantitatively describe the rISC pathway. Here we introduce a new, simple route to this end, just through explicit modeling and simulations on routinely available, experimental data from ultrafast transient absorption spectroscopy...
October 6, 2016: Journal of Physical Chemistry Letters
Jorge D Ramos, Martin F Casey, Aristotelis Bamias, Ugo De Giorgi, Joaquim Bellmunt, Lauren C Harshman, Sylvain Ladoire, Yu-Ning Wong, Ajjai S Alva, Jonathan E Rosenberg, Matthew D Galsky, Evan Y Yu
BACKGROUND: The Khorana score is a predictive risk model for venous thromboembolism (VTE) in patients with cancer planning to receive chemotherapy. Urothelial carcinoma and variant histologies (UC/VH) were underrepresented in the model. We sought to evaluate whether the Khorana score predicts for VTE in a retrospective multinational data set of patients with metastatic UC/VH. METHODS: Patients diagnosed with metastatic UC/VH who received chemotherapy were eligible...
September 16, 2016: Clinical and Applied Thrombosis/hemostasis
R Landin-Romero, P J McKenna, A Romaguera, E Álvarez-Moya, S Sarró, C Aguirre, C Sarri, A Compte, C Bosque, R Salvador, E Pomarol-Clotet
BACKGROUND: A key finding underlying the continuum of psychosis concept is the presence of psychotic-like experiences (PLEs) in healthy subjects. However, it remains uncertain to what extent these experiences are related to the genetic risk for schizophrenia and how far they actually resemble attenuated forms of psychotic symptoms. METHODS: Forty-nine adults with no history of mental illness in first-degree relatives and 59 siblings of patients with schizophrenia were rated on the psychosis section of the Computerized Diagnostic Interview Schedule IV (C DIS-IV) and the Rust Inventory of Schizotypal Cognitions (RISC)...
September 12, 2016: Schizophrenia Research
Noah I Hornick, Ben Doron, Sherif Abdelhamed, Jianya Huan, Christina A Harrington, Rongkun Shen, Xiaolu A Cambronne, Santhosh Chakkaramakkil Verghese, Peter Kurre
Exosomes are paracrine regulators of the tumor microenvironment and contain complex cargo. We previously reported that exosomes released from acute myeloid leukemia (AML) cells can suppress residual hematopoietic stem and progenitor cell (HSPC) function indirectly through stromal reprogramming of niche retention factors. We found that the systemic loss of hematopoietic function is also in part a consequence of AML exosome-directed microRNA (miRNA) trafficking to HSPCs. Exosomes isolated from cultured AML or the plasma from mice bearing AML xenografts exhibited enrichment of miR-150 and miR-155...
September 6, 2016: Science Signaling
Fu Yang, Rongwen Xi
Transposable elements or transposons are DNA pieces that can move around within the genome and are, therefore, potential threat to genome stability and faithful transmission of the genetic information in the germline. Accordingly, self-defense mechanisms have evolved in the metazoan germline to silence transposons, and the primary mechanism requires the germline-specific non-coding small RNAs, named Piwi-interacting RNA (piRNAs), which are in complex with Argonaute family of PIWI proteins (the piRNA-RISC complexes), to silence transposons...
September 6, 2016: Cellular and Molecular Life Sciences: CMLS
Yoichiro Ikushima, Takashi Hashido, Yoshiyuki Watanabe, Tsukasa Doi
PURPOSE: To quantitatively investigate in vitro the effects of flip angle (FA), receiver bandwidth (BW), echo time (TE), and magnetic field strength (FS) on image noise and artifacts induced by stent-assisted coiling on contrast-enhanced MR angiography (CE-MRA) images, as a first step towards optimization of imaging parameters. METHODS: A phantom simulating a cerebral aneurysm treated using stent-assisted coiling was filled with diluted gadolinium contrast medium, and MR angiography were obtained using varied parameters: FA (10°-60°), BW (164-780 Hz/pixel), and FS (1...
September 6, 2016: Magnetic Resonance in Medical Sciences: MRMS
Linghua Kong, Yun Liu, Guopeng Li, Yueyan Fang, Xiaofei Kang, Ping Li
OBJECTIVE: To examine the positive association between emotional intelligence and clinical communication ability among practice nursing students, and to determine whether resilience plays a moderating role in the relationship between emotional intelligence and clinical communication ability among Chinese practice nursing students. METHODS: Three hundred and seventy-seven practice nursing students from three hospitals participated in this study. They completed questionnaires including the Emotional Intelligence Inventory (EII), Connor-Davidson Resilience Scale (CD-RISC-10), and Clinical Communication Ability Scale (CCAS)...
November 2016: Nurse Education Today
A M Iannaccone, G Verrusio, S Iurassich
INTRODUCTION: Female genitalis lichen sclerosus (FGLS) occurs on skin and mucous membranes and shows inflammatory lesions, chronic atrophic, itching and pain. These physical damages produce a decline in sex and a resulting relational couple discomfort. AIM: We describe the discomfort and the relationship between physical and psychological damage. MATERIALS AND METHODS: A random sample, between 35-55 years (average 44.55, SD 6.00) includes 2 groups of 55 subjects: - the first with LSAG diagnosis since 24 months - the latter with controls...
July 2016: La Clinica Terapeutica
A M Iannaccone, G Verrusio, S Iurassich
INTRODUCTION: Psoriasis produces functional and aesthetic damages which cause psychosocial discomfort and require new adjustment factors. AIMS: We describe the correlation among discomfort and personality factors as traits, coping, defense mechanism (DM) and resilience in Ps (skin psoriasis) and PsA (psoriatic arthritis) patients. MATERIALS AND METHODS: A random sample, age between 45-60, with 1:1 female/male, includes 3 groups each of 90 subjects: - single psoriasis (Ps, average 50...
July 2016: La Clinica Terapeutica
Elizabeth Weathers, Rónán O'Caoimh, Ronan O'Sullivan, Constança Paúl, Frances Orfilia, Roger Clarnette, Carol Fitzgerald, Anton Svendrovski, Nicola Cornally, Patricia Leahy-Warren, D William Molloy
Predicting risk of adverse healthcare outcomes is important to enable targeted delivery of interventions. The Risk Instrument for Screening in the Community (RISC), designed for use by public health nurses (PHNs), measures the 1-year risk of hospitalisation, institutionalisation and death in community-dwelling older adults according to a five-point global risk score: from low (score 1,2) to medium (3) to high (4,5). We examined the inter-rater reliability (IRR) of the RISC between student PHNs (n=32) and expert raters using six cases (two low, medium and high-risk), scored before and after RISC training...
September 2016: British Journal of Community Nursing
Deepika Ahuja, Ashish Goyal, Partho Sarothi Ray
Tumor suppressor protein p53 plays a crucial role in maintaining genomic integrity in response to DNA damage. Regulation of translation of p53 mRNA is a major mode of regulation of p53 expression under genotoxic stress. The AU/U-rich element-binding protein HuR has been shown to bind to p53 mRNA 3'UTR and enhance translation in response to DNA-damaging UVC radiation. On the other hand, the microRNA miR-125b is reported to repress p53 expression and stress-induced apoptosis. Here, we show that UVC radiation causes an increase in miR-125b level in a biphasic manner, as well as nuclear cytoplasmic translocation of HuR...
September 3, 2016: RNA Biology
A N Madewell, E Ponce-Garcia, S E Martin
The data presented in this article are related to the article entitled "Assessing Resilience in Emerging Adulthood: The Resilience Scale (RS), Connor Davidson Resilience Scale (CD-RISC), and Scale of Protective Factors (SPF)" (Madewell and Ponce-Garcia, 2016) [1]. The data were collected from a sample of 451 college students from three universities located in the Southwestern region of the United States: 374 from a large public university and 67 from two smaller regional universities. The data from the three universities did not significantly differ in terms of demographics...
September 2016: Data in Brief
Bhaskar Roy, Michael Dunbar, Richard C Shelton, Yogesh Dwivedi
Major depressive disorder (MDD) is predicted to be the second leading cause of global disease burden by 2030. A large number of MDD patients do not respond to the currently available medication because of its poorly understood etiology. Recently, studies of microRNAs (miRNAs), which act as a molecular switch of gene expression, have shown promise in identifying a molecular network that could provide significant clues to various psychiatric illnesses. Using an in vitro system, a rodent depression model, and a human postmortem brain, we investigated the role of a brain-enriched, neuron-specific miRNA, miR-124-3p, whose expression is highly dysregulated in stressed rodents, and identified a set of target genes involved in stress response and neural plasticity...
October 5, 2016: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
Aaron Smith, John Calley, Sachin Mathur, Hui-Rong Qian, Han Wu, Mark Farmen, Florian Caiment, Pierre R Bushel, Jianying Li, Craig Fisher, Patrick Kirby, Erik Koenig, David G Hall, David E Watson
BACKGROUND: MicroRNAs (miRNA) are ~19-25 nucleotide long RNA molecules that fine tune gene expression through the inhibition of translation or degradation of the mRNA through incorporation into the RNA induced silencing complex (RISC). MicroRNAs are stable in the serum and plasma, are detectable in a wide variety of body fluids, are conserved across veterinary species and humans and are expressed in a tissue specific manner. They can be detected at low concentrations in circulation in animals and humans, generating interest in the utilization of miRNAs as serum and/or plasma based biomarkers of tissue injury...
2016: BMC Genomics
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