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https://www.readbyqxmd.com/read/29449323/structural-basis-of-sirna-recognition-by-trbp-double-stranded-rna-binding-domains
#1
Gregoire Masliah, Christophe Maris, Sebastian Lb König, Maxim Yulikov, Florian Aeschimann, Anna L Malinowska, Julie Mabille, Jan Weiler, Andrea Holla, Juerg Hunziker, Nicole Meisner-Kober, Benjamin Schuler, Gunnar Jeschke, Frederic H-T Allain
The accurate cleavage of pre-micro(mi)RNAs by Dicer and mi/siRNA guide strand selection are important steps in forming the RNA-induced silencing complex (RISC). The role of Dicer binding partner TRBP in these processes remains poorly understood. Here, we solved the solution structure of the two N-terminal dsRNA binding domains (dsRBDs) of TRBP in complex with a functionally asymmetric siRNA using NMR, EPR, and single-molecule spectroscopy. We find that siRNA recognition by the dsRBDs is not sequence-specific but rather depends on the RNA shape...
February 15, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29447113/the-ago-proteins-an-overview
#2
Saife Niaz
Small RNAs govern almost every biological process in eukaryotes associating with the Argonaute proteins to form the RNA-induced silencing complex (mRISC). Argonaute proteins constitute the core of RISCs with different members having variety of protein binding partners and biochemical properties. This review focuses on the AGO subfamily of the Argonautes that are ubiquitously expressed and are associated with small RNAs. The structure, function and the role of the AGO proteins in the cell is discussed in detail...
November 27, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29446422/basic-photophysical-analysis-of-a-thermally-activated-delayed-fluorescence-copper-i-complex-in-the-solid-state-theoretical-estimations-from-a-polarizable-continuum-model-pcm-tuned-range-separated-density-functional-approach
#3
Lingling Lv, Kun Yuan, Yongcheng Wang
A quantitative understanding of photophysical processes is fundamental for designing novel thermally activated delayed fluorescence (TADF) materials. Taking a Cu(pop)(pz 2 Bph 2 ) crystal as a typical TADF molecular model, we computed the conversion and decay rates of the first excited singlet state (S 1 ) and triplet state (T 1 ) at different temperatures by employing the thermal vibration correlation function (TVCF) approach. For the consideration of the solid-state environment, a methodology, which is based on the combination of a nonempirical, optimally tuned range-separated hybrid functional with the polarizable continuum model, was applied...
February 15, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29440232/noncoding-rnas-long-non-coding-rnas-and-micrornas-in-endocrine-related-cancers
#4
Carolyn M Klinge
The human genome is 'pervasively transcribed' leading to a complex array of non-coding RNAs (ncRNAs) that far outnumber coding mRNAs. ncRNAs have regulatory roles in transcription and post-transcriptional processes as well numerous cellular functions that remain to be fully described. Best characterized of the 'expanding universe' of ncRNAs are the ~ 22 nucleotide microRNAs (miRNAs) that base-pair to target mRNA's 3' untranslated region within the RNA-induced silencing complex (RISC) and block translation and may stimulate mRNA transcript degradation...
February 12, 2018: Endocrine-related Cancer
https://www.readbyqxmd.com/read/29435932/isolation-and-identification-of-gene-specific-micrornas
#5
Shi-Lung Lin, Donald C Chang, Shao-Yao Ying
Computer programming has identified hundreds of genomic hairpin sequences, many with functions yet to be determined. Because transfection of hairpin-like microRNA precursors (pre-miRNAs) into mammalian cells is not always sufficient to trigger RNA-induced gene silencing complex (RISC) assembly, a key step for inducing RNA interference (RNAi)-related gene silencing, we have developed an intronic miRNA expression system to overcome this problem by inserting a hairpin-like pre-miRNA structure into the intron region of a gene, and hence successfully increase the efficiency and effectiveness of miRNA-associated RNAi induction in vitro and in vivo...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435929/quantitative-analysis-of-precursors-micrornas-and-their-respective-mature-micrornas-in-cancer-exosomes-overtime
#6
Nuno Bastos, Sónia A Melo
Exosomes are a cell-to-cell communication system that transports information to neighbor and/or distant cells. Their content is composed of lipids, proteins, RNAs, and DNA. The role of exosomes released by cancer cells in disease progression has been widely studied. Here, we report a detailed protocol to quantify precursor microRNAs and mature microRNAs overtime in exosomes of cancer cells. Those precursor microRNAs that are packaged together with RISC proteins will get further processed in the exosomes overtime and an accumulation of the mature form of the microRNAs will occur...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435920/target-mrna-driven-biogenesis-of-cognate-micrornas-in-vitro
#7
Mainak Bose, Suvendra N Bhattacharyya
miRNAs are 20-22 nucleotide long noncoding RNAs that act as post-transcriptional regulators of gene expression controlling more than half of protein coding genes in humans. Being the critical modulators of the mRNA translation process, biogenesis, function, and turnover of these small RNAs are tightly regulated in cells. We have reported that target mRNAs induce increased biogenesis of cognate miRNAs from pre-miRNAs by increased activity of Ago-associated Dicer endonuclease that processes precursor miRNAs to their mature form...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29432194/structurally-modulated-codelivery-of-sirna-and-argonaute-2-for-enhanced-rna-interference
#8
Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Leemor Joshua-Tor, Paula T Hammond
Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we coencapsulate siRNA and the central RNAi effector protein Argonaute 2 (Ago2) via different delivery carriers as a platform to augment RNAi...
February 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29431916/clinical-relevance-of-smartphone-apps-for-diabetes-management-a-global-overview
#9
Zhilian Huang, Michael Soljak, Bernhard Otto Boehm, Josip Car
AIMS: We assessed the number, proportion and clinical relevance of diabetes self-management apps in major languages spoken by ten countries with the greatest number of people with diabetes. METHODS: China, India, USA, Brazil, Russian Federation, Mexico, Indonesia, Egypt, Japan and Pakistan were identified as the ten countries with the largest number of people with diabetes based on the latest NCD-RisC survey. Android and iOS apps in the ten national languages were extracted with a search strategy...
February 12, 2018: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/29413418/dise-a-seed-dependent-rnai-off-target-effect-that-kills-cancer-cells
#10
REVIEW
William Putzbach, Quan Q Gao, Monal Patel, Ashley Haluck-Kangas, Andrea E Murmann, Marcus E Peter
Off-target effects (OTEs) represent a significant caveat for RNAi caused by substantial complementarity between siRNAs and unintended mRNAs. We now discuss the existence of three types of seed-dependent OTEs (sOTEs). Type I involves unintended targeting through the guide strand seed of an siRNA. Type II is caused by the activity of the seed on the designated siRNA passenger strand when loaded into the RNA-induced silencing complex (RISC). Both type I and II sOTEs will elicit unpredictable cellular responses...
January 2018: Trends in Cancer
https://www.readbyqxmd.com/read/29397595/-the-impact-of-stress-and-personality-on-resilience-of-patients-with-ulcerative-colitis
#11
W Liu, J Wang, H Wang, X Y Chen, J S Li
Objective: To study relevant factors that influence psychological resilience in patients with ulcerative colitis(UC), especially the role of perceived stress and personality. Methods: Patients with UC were recruited from January 2015 to December 2016 in the First Hospital of Zhengzhou University. Education levels, income, duration of disease, Mayo score and disease phenotype according to Montreal classification were collected. Resilience was measured using Connor-Davidson resilience scale (CD-RISC). Perceived stress was measured by perceived stress scale (PSS)...
February 1, 2018: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/29386283/modified-cross-linking-ligation-and-sequencing-of-hybrids-qclash-identifies-kshv-microrna-targets-in-endothelial-cells
#12
Lauren A Gay, Sunantha Sethuraman, Merin Thomas, Peter C Turner, Rolf Renne
KS tumors are derived from endothelial cells and express KHSV microRNAs (miRNAs). Although miRNA targets have been identified in B cell lymphoma-derived cells and in epithelial cells, little has been done to characterize the KSHV miRNA targetome in endothelial cells. A recent innovation in the identification of miRNA targetomes, Cross-linking, Ligation, and Sequencing of Hybrids (CLASH), unambiguously identifies miRNAs and their targets by ligating the two species while both are still bound within the RNA-Induced Silencing Complex (RISC)...
January 31, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29385985/resilience-and-burden-in-caregivers-of-older-adults-moderating-and-mediating-effects-of-perceived-social-support
#13
Hui Lin Ong, Janhavi Ajit Vaingankar, Edimansyah Abdin, Rajeswari Sambasivam, Restria Fauziana, Min-En Tan, Siow Ann Chong, Richard Roshan Goveas, Peak Chiang Chiam, Mythily Subramaniam
BACKGROUND: The burden of caring for an older adult can be a form of stress and influence caregivers' daily lives and health. Previous studies have reported that resilience and social support play an important role in reducing physical and psychological burden in caregivers. Thus, the present study aimed to examine whether perceived social support served as a possible protective factor of burden among caregivers of older adults in Singapore using moderation and mediation effects' models...
January 31, 2018: BMC Psychiatry
https://www.readbyqxmd.com/read/29380708/technology-of-rna-interference-in-advanced-medicine
#14
Fatemeh Abedini, Mohammad Ebrahimi, Hossein Hosseinkhani
RNA interference (RNAi) and related pathways involving small interfering RNAs (siRNAs), microRNAs (miRNAs), and PIWI-interacting RNAs (piRNAs) regulate processes such as anti-viral defense, genome surveillance, heterochromatin formation, and gene expression in animals, plants, and fungi. Studies on RNAi have revealed a two-step mechanism: (i) Degradation of dsRNA into small interfering RNAs (siRNAs), 21 to 25 nucleotides long, by an RNase III-like activity. (ii) The siRNAs join an RNase complex, RISC (RNA-induced silencing complex), which acts on the mRNA and degrades it...
January 29, 2018: MicroRNA
https://www.readbyqxmd.com/read/29376650/facile-synthesis-geometry-and-2-substituent-dependent-in-vivo-activity-of-5-e-and-5-z-vinylphosphonate-modified-sirna-conjugates
#15
Rubina Giare Parmar, Christopher R Brown, Shigeo Matsuda, Jennifer L S Willoughby, Christopher S Theile, Klaus Charissé, Donald J Foster, Ivan Zlatev, Vasant Jadhav, Martin A Maier, Martin Egli, Muthiah Manoharan, Kallanthottathil G Rajeev
(E)-Vinylphosphonate ((E)-VP), a metabolically stable phosphate mimic at the 5'-end of the antisense strand, enhances the in vivo potency of siRNA. Here we describe a straightforward synthetic approach to incorporate a nucleotide carrying a vinylphosphonate (VP) moiety at the 5'-end of oligonucleotides under standard solid-phase synthesis and deprotection conditions by utilizing pivaloyloxymethyl (POM) protected VP-nucleoside phosphoramidites. The POM protection enhances scope and scalability of 5'-VP-modified oligonucleotides and, in a broader sense, the synthesis of oligonucleotides modified with phosphonate moieties...
January 27, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29357779/adversity-and-resilience-are-associated-with-outcome-following-mild-traumatic-brain-injury-in-military-service-members
#16
Matthew Wade Reid, Douglas Cooper, Lisa H Lu, Grant L Iverson, Jan Kennedy
The objective of this study was to assess the associations between resilience, adversity, post-concussion symptoms, and posttraumatic stress symptom reporting following mild traumatic brain injury (mTBI). We hypothesized that resilience would be associated with less symptom reporting and adversity would be associated with greater symptom reporting. This was a cross-sectional study of retrospective data collected for an ongoing TBI repository. US military service members who screened positive for mTBI during a primary care visit completed the Trauma History Screen (THS), Connor-Davidson Resilience Scale (CD-RISC), Neurobehavioral Symptom Inventory (NSI), and PTSD Checklist-Civilian Version (PCL-C)...
January 22, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29353293/alcohol-tobacco-and-cannabis-consumption-in-adolescents-from-a-multicultural-population-burela-lugo
#17
Ainara Díaz Geada, Alicia Busto Miramontes, Francisco Caamaño Isorna
Social inequalities have been associated with morbidity and mortality. Gender, ethnic group and inequalities were studied in an adolescent population to analyze alcohol, tobacco and cannabis consumption. We carried out a cross-sectional study of pupils from high schools in Burela (northern Spain) (n=238). We used the "Factors de Risc en Estudiants de Secundária" questionnaire designed by Agència de Salut Pública de Barcelona. INDEPENDENT VARIABLES: nationality and weekly pocket money...
January 15, 2018: Adicciones
https://www.readbyqxmd.com/read/29351856/high-density-lipoprotein-with-apolipoprotein-c-iii-is-associated-with-carotid-intima-media-thickness-among-generally-healthy-individuals
#18
Rain Yamamoto, Frank M Sacks, Frank B Hu, Bernard Rosner, Jeremy D Furtado, Sarah A Aroner, Ele Ferrannini, Simona Baldi, Michaela Kozakova, Beverley Balkau, Andrea Natali, Majken K Jensen
BACKGROUND AND AIMS: About 6-7% of high density lipoprotein (HDL) has a protein called apolipoprotein (apo) C-III that regulates lipoprotein metabolism and can provoke an inflammatory response. HDL without apoC-III is inversely associated with coronary heart disease (CHD), whereas HDL with apoC-III is directly associated with CHD. We investigated how the presence of apoC-III affects the association between HDL and early stages of atherosclerosis measured as carotid intima-media thickness (cIMT)...
January 2, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29335041/clinical-correlates-of-resilience-factors-in-geriatric-depression
#19
Kelsey T Laird, Helen Lavretsky, Pattharee Paholpak, Roza M Vlasova, Michael Roman, Natalie St Cyr, Prabha Siddarth
BACKGROUND: Traditional perspectives conceptualize resilience as a trait and depression as resulting from resilience deficiency. However, research indicates that resilience varies substantially even among adults who are clinically depressed, as well as across the lifespan of an individual. Few studies have investigated resilience in depression, and even fewer have examined resilience in depressed older adults. METHODS: Three hundred thirty-seven adults ≥60 years with major depressive disorder completed the Connor-Davidson Resilience Scale (CD-RISC) and measures of mental health, quality of life (QOL), and medical comorbidity...
January 16, 2018: International Psychogeriatrics
https://www.readbyqxmd.com/read/29329529/resilience-and-well-being-of-university-nursing-students-in-hong-kong-a-cross-sectional-study
#20
Ka Ming Chow, Wing Ki Fiona Tang, Wing Han Carmen Chan, Wing Hung Janet Sit, Kai Chow Choi, Sally Chan
BACKGROUND: University nursing students experience higher levels of academic stress than those of other disciplines. Academic stress leads to psychological distress and has detrimental effects on well-being. The ability to overcome such adversity and learn to be stronger from the experience is regarded as resilience. Resilience is found to have an impact on learning experience, academic performance, course completion and, in the longer term, professional practice. Resilience and positive coping strategies can resist stress and improve personal well-being...
January 12, 2018: BMC Medical Education
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