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https://www.readbyqxmd.com/read/27911494/determining-functional-aptamer-protein-interaction-by-biolayer-interferometry
#1
Xinhui Lou, Martin Egli, Xianbin Yang
Short single-stranded nucleic acids called aptamers are widely being explored as recognition molecules of high affinity and specificity for binding a wide range of target molecules, particularly protein targets. In biolayer interferometry (BLI), a simple Dip-and-Read approach in which the aptamer-coated biosensors are dipped into microplate wells is used to study the interactions between an aptamer and its target protein. Here we describe the protocol for the analysis of the interaction between a well-characterized anti-thrombin RNA aptamer with thrombin (Basic Protocol)...
December 1, 2016: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/27901046/revealing-the-spin-vibronic-coupling-mechanism-of-thermally-activated-delayed-fluorescence
#2
Marc K Etherington, Jamie Gibson, Heather F Higginbotham, Thomas J Penfold, Andrew P Monkman
Knowing the underlying photophysics of thermally activated delayed fluorescence (TADF) allows proper design of high efficiency organic light-emitting diodes. We have proposed a model to describe reverse intersystem crossing (rISC) in donor-acceptor charge transfer molecules, where spin-orbit coupling between singlet and triplet states is mediated by one of the local triplet states of the donor (or acceptor). This second order, vibronically coupled mechanism describes the basic photophysics of TADF. Through a series of measurements, whereby the energy ordering of the charge transfer (CT) excited states and the local triplet are tuned in and out of resonance, we show that TADF reaches a maximum at the resonance point, substantiating our model of rISC...
November 30, 2016: Nature Communications
https://www.readbyqxmd.com/read/27896228/the-european-innovation-partnership-on-active-and-healthy-ageing-synergies-protocol-for-a-prospective-observational-study-to-measure-the-impact-of-a-community-based-program-on-prevention-and-mitigation-of-frailty-icp-pmf-in-community-dwelling-older-adults
#3
G Liotta, F Orfila, M Vollenbroek-Hutten, R Roller-Winsberger, M Illario, D Musian, S Alvino, R O'Caoimh, A Cano, W Molloy, G Iaccarino, M C Marazzi, M C Inzerilli, O Madaro, C Paul, P Csonka, A C Vince, E Menditto, M Maggio, P Scarcella, F Gilardi, F Lucaroni, P Abete, V Girardi, R Barra, L Palombi
Aim of this paper is to describe the protocol of the study "Impact of a Community-based Program on Prevention and Mitigation of Frailty in community-dwelling older adults" developed in the framework of the European Innovation Partnership on Active and Healthy Ageing. This proposal has been developed by the Partnership Action groups on frailty, fall prevention and polypharmacy in older. The proposal wants to assess the impact of community-based programs aimed to counteract three main outcomes related to frailty: hospitalization, institutionalization and death...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27868331/patient-resilience-in-the-fracture-orthopaedic-rehabilitation-geriatric-environment
#4
Sabrina Kohler, Sze Ming Loh
OBJECTIVE: To explore the relationship between resilience and rehabilitation outcomes in older orthopaedic patients. METHODS: Geriatric rehabilitation patients admitted to a general metropolitan hospital following a fracture were interviewed face-to-face. Their resilience was assessed using the Connor-Davidson Resilience Scale (CD-RISC), and rehabilitation outcomes were assessed according to functional independence measure (FIM) gain, length of stay, discharge destination and mortality rate...
November 20, 2016: Australasian Journal on Ageing
https://www.readbyqxmd.com/read/27863813/worldwide-trends-in-blood-pressure-from-1975-to-2015-a-pooled-analysis-of-1479-population-based-measurement-studies-with-19%C3%A2-1-million-participants
#5
(no author information available yet)
BACKGROUND: Raised blood pressure is an important risk factor for cardiovascular diseases and chronic kidney disease. We estimated worldwide trends in mean systolic and mean diastolic blood pressure, and the prevalence of, and number of people with, raised blood pressure, defined as systolic blood pressure of 140 mm Hg or higher or diastolic blood pressure of 90 mm Hg or higher. METHODS: For this analysis, we pooled national, subnational, or community population-based studies that had measured blood pressure in adults aged 18 years and older...
November 15, 2016: Lancet
https://www.readbyqxmd.com/read/27837013/partial-reconstitution-of-the-rnai-response-in-human-cells-using-drosophila-gene-products
#6
Edward M Kennedy, Anand V R Kornepati, Hal P Bogerd, Bryan R Cullen
While mammalian somatic cells are incapable of mounting an effective RNA interference (RNAi) response to viral infections, plants and invertebrates are able to generate high levels of viral short interfering RNAs (siRNAs) that can control many infections. In Drosophila, the RNAi response is mediated by the Dicer 2 enzyme (dDcr2) acting in concert with two co-factors called Loqs-PD and R2D2. To examine whether a functional RNAi response could be mounted in human somatic cells, we expressed dDcr2, in the presence or absence of Loqs-PD and/or R2D2, in a previously described human cell line, NoDice/ΔPKR, that lacks functional forms of human Dicer (hDcr) and PKR...
November 11, 2016: RNA
https://www.readbyqxmd.com/read/27825278/resilience-associated-with-mental-health-problems-among-methadone-maintenance-treatment-patients-in-guangzhou-china
#7
Mingxu Jiao, Jing Gu, Huifang Xu, Chun Hao, Joseph T F Lau, Phoenix Mo, Di Liu, Yuteng Zhao, Xiao Zhang, Andrew Babbitt, Yuantao Hao
A considerable proportion of methadone maintenance treatment (MMT) clients have experienced mental health problems (e.g., depression and anxiety), and poor mental health status is associated with HIV-related risk behaviors and treatment drop-out. Resilience is known to be a protective factor for mental health problems but is not studied among MMT clients in China. This study aimed to explore the relationship between resilience and mental health problems (depression, anxiety and stress) among clients of community-based MMT clinics in China...
November 8, 2016: AIDS Care
https://www.readbyqxmd.com/read/27823722/relationship-between-prenatal-maternal-stress-and-sleep-quality-in-chinese-pregnant-women-the-mediation-effect-of-resilience
#8
Guopeng Li, Linghua Kong, Haiyan Zhou, Xiaofei Kang, Yueyan Fang, Ping Li
OBJECTIVE: To examine the relationship between prenatal maternal stress, resilience, and sleep quality, and to determine whether resilience plays a mediating role in the relationship between prenatal maternal stress and sleep quality among pregnant women. METHODS: Two hundred and thirty-one pregnant women in their second trimester participated in the study. They completed questionnaires, including: the Pittsburgh Sleep Quality Index (PSQI), the Pregnancy Stress Rating Scale (PSRS), and the 10-item Connor-Davidson Resilience Scale (CD-RISC-10)...
September 2016: Sleep Medicine
https://www.readbyqxmd.com/read/27806483/the-effectiveness-of-dohsa-psycho-motor-rehabilitation-method-on-fatigue-severity-sleep-quality-and-resilience-promotion-of-patients-with-multiple-sclerosis-ms
#9
Azam Azari, Hamid Kazemi-Zahrani, Masoumeh Mohammadi Khashouei
Because of multiple psychological-physical symptoms and failure to accept the reality, multiple sclerosis (MS), patients are suffering from negative mood disorders and fatigue which affects their life quality negatively. Therefore this study has been conducted to determine the effect of Dohsa Psycho-Motor Rehabilitation Method on fatigue severity, sleep quality, and Resilience promotion of Patients with Multiple Sclerosis in Isfahan, Iran. A quasi-experimental study with pre-test, post-test and follow up was administered on both the experimental and control groups...
July 5, 2016: Global Journal of Health Science
https://www.readbyqxmd.com/read/27803482/development-of-novel-genetically-engineered-adenoviruses-based-on-functional-analyses-of-adenovirus-encoded-small-rnas
#10
Mitsuhiro Machitani
 The adenovirus (Ad) genome encodes two small noncoding RNAs, VA-RNA I and II, which support Ad replication by antagonizing the antiviral action associated with the Ad-induced activation of double-stranded RNA-dependent protein kinase (PKR). VA-RNAs are also processed in a manner similar to microRNAs (miRNAs), resulting in the production of VA-RNA-derived miRNAs (mivaRNAs). mivaRNAs are incorporated into the RNA-induced silencing complex (RISC) and exhibit posttranscriptional silencing in a manner similar to miRNAs...
2016: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/27801781/function-and-regulation-of-micrornas-and-their-potential-as-biomarkers-in-paediatric-liver-disease
#11
REVIEW
Diego A Calvopina, Miranda A Coleman, Peter J Lewindon, Grant A Ramm
MicroRNAs (miRNAs) are short non-coding RNAs involved in biological and pathological processes of every cell type, including liver cells. Transcribed from specific genes, miRNA precursors are processed in the cytoplasm into mature miRNAs and as part of the RNA-induced silencing complex (RISC) complex binds to messenger RNA (mRNA) by imperfect complementarity. This leads to the regulation of gene expression at a post-transcriptional level. The function of a number of different miRNAs in fibrogenesis associated with the progression of chronic liver disease has recently been elucidated...
October 27, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27783951/a-nuclear-role-for-mir-9-and-argonaute-proteins-in-balancing-quiescent-and-activated-neural-stem-cell-states
#12
Shauna Katz, Delphine Cussigh, Noelia Urbán, Isabelle Blomfield, François Guillemot, Laure Bally-Cuif, Marion Coolen
Throughout life, adult neural stem cells (NSCs) produce new neurons and glia that contribute to crucial brain functions. Quiescence is an essential protective feature of adult NSCs; however, the establishment and maintenance of this state remain poorly understood. We demonstrate that in the adult zebrafish pallium, the brain-enriched miR-9 is expressed exclusively in a subset of quiescent NSCs, highlighting a heterogeneity within these cells, and is necessary to maintain NSC quiescence. Strikingly, miR-9, along with Argonaute proteins (Agos), is localized to the nucleus of quiescent NSCs, and manipulating their nuclear/cytoplasmic ratio impacts quiescence...
October 25, 2016: Cell Reports
https://www.readbyqxmd.com/read/27726966/nomogram-based-prediction-of-overall-survival-in-patients-with-metastatic-urothelial-carcinoma-receiving-first-line-platinum-based-chemotherapy-retrospective-international-study-of-invasive-advanced-cancer-of-the-urothelium-risc
#13
Andrea Necchi, Guru Sonpavde, Salvatore Lo Vullo, Daniele Giardiello, Aristotelis Bamias, Simon J Crabb, Lauren C Harshman, Joaquim Bellmunt, Ugo De Giorgi, Cora N Sternberg, Linda Cerbone, Sylvain Ladoire, Yu-Ning Wong, Evan Y Yu, Simon Chowdhury, Gunter Niegisch, Sandy Srinivas, Ulka N Vaishampayan, Sumanta K Pal, Neeraj Agarwal, Ajjai Alva, Jack Baniel, Ali-Reza Golshayan, Rafael Morales-Barrera, Daniel W Bowles, Matthew I Milowsky, Christine Theodore, Dominik R Berthold, Gedske Daugaard, Srikala S Sridhar, Thomas Powles, Jonathan E Rosenberg, Matthew D Galsky, Luigi Mariani
BACKGROUND: The available prognostic models for overall survival (OS) in patients with metastatic urothelial carcinoma (UC) have been derived from clinical trial populations of cisplatin-treated patients. OBJECTIVE: To develop a new model based on real-world patients. DESIGN, SETTING, AND PARTICIPANTS: Individual patient-level data from 29 centers were collected, including metastatic UC and first-line cisplatin- or carboplatin-based chemotherapy administered between January 2006 and January 2011...
October 8, 2016: European Urology
https://www.readbyqxmd.com/read/27714245/groove-modification-of-sirna-duplexes-to-elucidate-sirna-protein-interactions-using-7-bromo-7-deazaadenosine-and-3-bromo-3-deazaadenosine-as-chemical-probes
#14
Noriko Saito-Tarashima, Hirotaka Kira, Tomoya Wada, Kazuya Miki, Shiho Ide, Naoshi Yamazaki, Akira Matsuda, Noriaki Minakawa
Elucidation of dynamic interactions between RNA and proteins is essential for understanding the biological processes regulated by RNA, such as RNA interference (RNAi). In this study, the logical chemical probes, comprising 7-bromo-7-deazaadenosine (Br(7)C(7)A) and 3-bromo-3-deazaadenosine (Br(3)C(3)A), to investigate small interfering RNA (siRNA)-RNAi related protein interactions, were developed. The bromo substituents of Br(7)C(7)A and Br(3)C(3)A are expected to be located in the major and the minor grooves, respectively, and to act as a steric hindrance in each groove when these chemical probes are incorporated into siRNAs...
September 28, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27708832/dispositional-mindfulness-and-employment-status-as-predictors-of-resilience-in-third-year-nursing-students-a-quantitative-study
#15
Diane Chamberlain, Allison Williams, David Stanley, Peter Mellor, Wendy Cross, Lesley Siegloff
BACKGROUND: Nursing students will graduate into stressful workplace environments and resilience is an essential acquired ability for surviving the workplace. Few studies have explored the relationship between resilience and the degree of innate dispositional mindfulness, compassion, compassion fatigue and burnout in nursing students, including those who find themselves in the position of needing to work in addition to their academic responsibilities. AIM: This paper investigates the predictors of resilience, including dispositional mindfulness and employment status of third year nursing students from three Australian universities...
October 2016: Nursing Open
https://www.readbyqxmd.com/read/27708205/the-classification-structure-and-functioning-of-ago-proteins-in-eukaryotes
#16
Aleksandra Poterala, Joanna Rzeszowska-Wolny
Ago proteins are members of the highly specialized and conserved Argonaute family, primarily responsible for regulation of gene expression. As a part of RNA-induced silencing complexes (RISCs) Ago proteins are responsible for binding a short RNA and cleavage/inhibition of translation of target mRNAs. Phosphorylation may work as the switch between those two functions, but the role of magnesium ion concentration is also taken into consideration. Recent reports indicate that Ago proteins can interact with an mRNA and cause inhibition of translation without the participation of a short RNA...
September 28, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/27682680/6-hydroxydopamine-inhibits-the-hepatitis-c-virus-through-alkylation-of-host-and-viral-proteins-and-the-induction-of-oxidative-stress
#17
Matthew A Lafreniere, Megan H Powdrill, Ragunath Singaravelu, John Paul Pezacki
Many viruses, including the hepatitis C virus (HCV), are dependent on the host RNA silencing pathway for replication. In this study, we screened small molecule probes, previously reported to disrupt loading of the RNA-induced silencing complex (RISC), including 6-hydroxydopamine (6-OHDA), suramin (SUR), and aurintricarboxylic acid (ATA), to examine their effects on viral replication. We found that 6-OHDA inhibited HCV replication; however, 6-OHDA was a less potent inhibitor of RISC than either SUR or ATA. By generating a novel chemical probe (6-OHDA-yne), we determined that 6-OHDA covalently modifies host and virus proteins...
October 13, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27651455/phosphorylation-specific-status-of-rnai-triggers-in-pharmacokinetic-and-biodistribution-analyses
#18
Vladimir S Trubetskoy, Jacob B Griffin, Anthony L Nicholas, Eric M Nord, Zhao Xu, Ryan M Peterson, Christine I Wooddell, David B Rozema, Darren H Wakefield, David L Lewis, Steven B Kanner
The RNA interference (RNAi)-based therapeutic ARC-520 for chronic hepatitis B virus (HBV) infection consists of a melittin-derived peptide conjugated to N-acetylgalactosamine for hepatocyte targeting and endosomal escape, and cholesterol-conjugated RNAi triggers, which together result in HBV gene silencing. To characterize the kinetics of RNAi trigger delivery and 5'-phosphorylation of guide strands correlating with gene knockdown, we employed a peptide-nucleic acid (PNA) hybridization assay. A fluorescent sense strand PNA probe binding to RNAi duplex guide strands was coupled with anion exchange high performance liquid chromatography to quantitate guide strands and metabolites...
September 19, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27649268/retrieving-the-rate-of-reverse-intersystem-crossing-from-ultrafast-spectroscopy
#19
Jiahua Hu, Qun Zhang, Yi Luo
Among various nonradiative photophysical processes related to energy migration in singlet-triplet coupled molecular systems, unlike such processes as internal conversion, intersystem crossing (ISC), and intramolecular vibrational relaxation that have been extensively addressed, the reverse ISC (rISC) is a unique one that apparently lacks sufficient interrogation probably owing to its intrinsically elusive nature. In particular, it still remains a nontrivial task to quantitatively describe the rISC pathway. Here we introduce a new, simple route to this end, just through explicit modeling and simulations on routinely available, experimental data from ultrafast transient absorption spectroscopy...
October 6, 2016: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/27637910/the-khorana-score-in-predicting-venous-thromboembolism-for-patients-with-metastatic-urothelial-carcinoma-and-variant-histology-treated-with-chemotherapy
#20
Jorge D Ramos, Martin F Casey, Aristotelis Bamias, Ugo De Giorgi, Joaquim Bellmunt, Lauren C Harshman, Sylvain Ladoire, Yu-Ning Wong, Ajjai S Alva, Jonathan E Rosenberg, Matthew D Galsky, Evan Y Yu
BACKGROUND: The Khorana score is a predictive risk model for venous thromboembolism (VTE) in patients with cancer planning to receive chemotherapy. Urothelial carcinoma and variant histologies (UC/VH) were underrepresented in the model. We sought to evaluate whether the Khorana score predicts for VTE in a retrospective multinational data set of patients with metastatic UC/VH. METHODS: Patients diagnosed with metastatic UC/VH who received chemotherapy were eligible...
September 16, 2016: Clinical and Applied Thrombosis/hemostasis
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