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Muscle molecular metabolism

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https://www.readbyqxmd.com/read/29679381/comparative-gel-based-proteomic-analysis-of-chemically-crosslinked-complexes-in-dystrophic-skeletal-muscle
#1
Sandra Murphy, Margit Zweyer, Rustam R Mundegar, Dieter Swandulla, Kay Ohlendieck
Duchenne muscular dystrophy is a highly progressive muscle wasting disease with a complex pathophysiology that is based on primary abnormalities in the dystrophin gene. In order to study potential changes in the oligomerisation of high-molecular-mass protein complexes in dystrophic skeletal muscle, chemical crosslinking was combined with mass spectrometric analysis. The biochemical stabilization of protein interactions was carried out with the homo-bifunctional and amine-reactive agent bis[sulfosuccinimidyl]suberate, followed by protein shift analysis in one-dimensional gels...
April 20, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29678953/metabolic-adjustment-to-high-altitude-hypoxia-from-genetic-signals-to-physiological-implications
#2
REVIEW
Andrew J Murray, Hugh E Montgomery, Martin Feelisch, Michael P W Grocott, Daniel S Martin
Ascent to high altitude is associated with physiological responses that counter the stress of hypobaric hypoxia by increasing oxygen delivery and by altering tissue oxygen utilisation via metabolic modulation. At the cellular level, the transcriptional response to hypoxia is mediated by the hypoxia-inducible factor (HIF) pathway and results in promotion of glycolytic capacity and suppression of oxidative metabolism. In Tibetan highlanders, gene variants encoding components of the HIF pathway have undergone selection and are associated with adaptive phenotypic changes, including suppression of erythropoiesis and increased blood lactate levels...
April 20, 2018: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29674374/changes-in-free-amino-acid-concentrations-and-associated-gene-expression-profiles-in-the-abdominal-muscle-of-kuruma-shrimp-marsupenaeus-japonicus-acclimated-at-different-salinities
#3
Hiroki Koyama, Nanami Mizusawa, Masataka Hoashi, Engkong Tan, Ko Yasumoto, Mitsuru Jimbo, Daisuke Ikeda, Takehiko Yokoyama, Shuichi Asakawa, Sanit Piyapattanakorn, Shugo Watabe
Shrimps inhabiting the coastal water can survive in a wide range of salinity. However, the molecular mechanisms involved in their acclimation to different environmental salinities have remained largely unknown. In the present study, we acclimated kuruma shrimp Marsupenaeus japonicus at 1.7 %, 3.4 % and 4.0 % salinities. After acclimating for 6, 12, 24 and 72 h, we determined free amino acid concentrations in their abdominal muscle, and performed RNA-seq analysis on this muscle. The concentrations of free amino acids were clearly altered depending on salinity after acclimating for 24 h...
April 19, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29674282/bioenergetics-mechanisms-regulating-muscle-stem-cell-self-renewal-commitment-and-function
#4
REVIEW
Phablo Abreu
Muscle stem cells or satellite cells are crucial for muscle maintenance and repair. These cells are mitotically quiescent and uniformly express the transcription factor Pax7, intermittently entering the cell cycle to give rise to daughter myogenic precursors cells and fuse with neighboring myofibers or self-renew, replenishing the stem cell pool in adult skeletal muscle. Pivotal roles of muscle stem cells in muscle repair have been uncovered, but it still remains unclear how muscle stem cell self-renewal is molecularly regulated and how muscle stem cells maintain muscle tissue homeostasis...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29672225/probiotic-supplementation-in-trained-trotter-horses-effect-on-blood-clinical-pathology-data-and-urine-metabolomic-assessed-in-field
#5
Luca Laghi, Chenglin Zhu, Giuseppe Campagna, Giacomo Rossi, Marilena Bazzano, Fulvio Laus
The attention of sports community towards probiotic supplementation as a way to promote exercise and training performance, together with good health, has increased in recent years. This has applied also to horses, with promising results. Here, for the first time, we tested a probiotic mix of several strains of alive bacteria typically employed for humans to improve the training performance of Standardbred horses in athletic activity. To evaluate its effects on the horse performance, we measured lactate concentration in blood, a translational outcome largely employed for the purpose, combined with the study of hematological and biochemical parameters, together with urine from a metabolomics perspective...
April 19, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29670524/semen-cassiae-extract-improves-glucose-metabolism-by-promoting-glut4-translocation-in-the-skeletal-muscle-of-diabetic-rats
#6
Meiling Zhang, Xin Li, Hangfei Liang, Huqiang Cai, Xueling Hu, Yu Bian, Lei Dong, Lili Ding, Libo Wang, Bo Yu, Yan Zhang, Yao Zhang
Diabetes mellitus is a clinical syndrome characterised by hyperglycaemia; its complications lead to disability and even death. Semen Cassiae is a traditional Chinese medicine, which has anti-hypertensive, anti-hyperlipidaemia, anti-oxidation, and anti-ageing properties. Our study was designed to evaluate the action of total anthraquinones of Semen Cassiae extract (SCE) on the improvement of glucose metabolism in diabetic rats and to elucidate the underlying mechanism. First, we evaluated the effect of SCE on normal rats...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29664058/relationships-between-rapid-eye-movement-sleep-behavior-disorder-and-neurodegenerative-diseases-clinical-assessments-biomarkers-and-treatment
#7
REVIEW
Min Li, Li Wang, Jiang-Hong Liu, Shu-Qin Zhan
Objective: Rapid eye movement sleep behavior disorder (RBD) is characterized by dream enactment and loss of muscle atonia during rapid eye movement sleep. RBD is closely related to α-synucleinopathies including Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy. Many studies have investigated the markers of imaging and neurophysiological, genetic, cognitive, autonomic function of RBD and their predictive value for neurodegenerative diseases. This report reviewed the progress of these studies and discussed their limitations and future research directions...
April 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29662503/identification-and-functional-analysis-of-long-intergenic-non-coding-rnas-underlying-intramuscular-fat-content-in-pigs
#8
Cheng Zou, Long Li, Xiaofang Cheng, Cencen Li, Yuhua Fu, Chengchi Fang, Changchun Li
Intramuscular fat (IMF) content is an important trait that can affect pork quality. Previous studies have identified many genes that can regulate IMF. Long intergenic non-coding RNAs (lincRNAs) are emerging as key regulators in various biological processes. However, lincRNAs related to IMF in pig are largely unknown, and the mechanisms by which they regulate IMF are yet to be elucidated. Here we reconstructed 105,687 transcripts and identified 1,032 lincRNAs in pig longissimus dorsi muscle (LDM) of four stages with different IMF contents based on published RNA-seq...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29657030/rewiring-of-glutamine-metabolism-is-a-bioenergetic-adaptation-of-human-cells-with-mitochondrial-dna-mutations
#9
Qiuying Chen, Kathryne Kirk, Yevgeniya I Shurubor, Dazhi Zhao, Andrea J Arreguin, Ifrah Shahi, Federica Valsecchi, Guido Primiano, Elizabeth L Calder, Valerio Carelli, Travis T Denton, M Flint Beal, Steven S Gross, Giovanni Manfredi, Marilena D'Aurelio
Using molecular, biochemical, and untargeted stable isotope tracing approaches, we identify a previously unappreciated glutamine-derived α-ketoglutarate (αKG) energy-generating anaplerotic flux to be critical in mitochondrial DNA (mtDNA) mutant cells that harbor human disease-associated oxidative phosphorylation defects. Stimulating this flux with αKG supplementation enables the survival of diverse mtDNA mutant cells under otherwise lethal obligatory oxidative conditions. Strikingly, we demonstrate that when residual mitochondrial respiration in mtDNA mutant cells exceeds 45% of control levels, αKG oxidative flux prevails over reductive carboxylation...
April 9, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29656554/a-single-day-of-bed-rest-irrespective-of-energy-balance-does-not-affect-skeletal-muscle-gene-expression-or-insulin-sensitivity
#10
Marlou L Dirks, Francis B Stephens, Sarah R Jackman, Jesús Galera Gordo, David Machin, Richard M Pulsford, Luc Jc van Loon, Benjamin T Wall
NEW FINDINGS: What is the central question of this study? What are the initial metabolic and molecular events that underpin bed rest-induced skeletal muscle deconditioning, and what is the contribution of energy balance? What is the main finding and its importance? A single day of bed rest, irrespective of energy balance, did not lead to overt changes in skeletal muscle gene expression or insulin sensitivity. More than one day of physical inactivity is required to observe the insulin resistance and robust skeletal muscle transcriptional responses associated with bed rest and consequent alterations in energy balance...
April 15, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29653260/comparative-proteomic-analysis-of-fluoride-treated-rat-bone-provides-new-insights-into-the-molecular-mechanisms-of-fluoride-toxicity
#11
Yan Wei, Beibei Zeng, Hua Zhang, Cheng Chen, Yanli Wu, Nanlan Wang, Yanqiu Wu, Danqing Zhao, Yuxi Zhao, Javed Iqbal, Liming Shen
Long-term excessive intake of fluoride (F) could lead to chronic fluorosis. To explore the underlying molecular mechanisms, present study is designed to elucidate the effect of fluoride on proteome expression of bone in sodium fluoride (NaF)-treated SD rats. Hematoxylin and eosin (H&E) staining was used to determine the severity of osteofluorosis, and bone samples were submitted for iTRAQ analysis. The results showed that the cortical thickness and trabecular area of femur bone in medium- and high-dose groups were higher than in control group...
April 10, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29617655/ptcd1-is-required-for-16s-rrna-maturation-complex-stability-and-mitochondrial-ribosome-assembly
#12
Kara L Perks, Giulia Rossetti, Irina Kuznetsova, Laetitia A Hughes, Judith A Ermer, Nicola Ferreira, Jakob D Busch, Danielle L Rudler, Henrik Spahr, Thomas Schöndorf, Ann-Marie J Shearwood, Helena M Viola, Stefan J Siira, Livia C Hool, Dusanka Milenkovic, Nils-Göran Larsson, Oliver Rackham, Aleksandra Filipovska
The regulation of mitochondrial RNA life cycles and their roles in ribosome biogenesis and energy metabolism are not fully understood. We used CRISPR/Cas9 to generate heart- and skeletal-muscle-specific knockout mice of the pentatricopeptide repeat domain protein 1, PTCD1, and show that its loss leads to severe cardiomyopathy and premature death. Our detailed transcriptome-wide and functional analyses of these mice enabled us to identify the molecular role of PTCD1 as a 16S rRNA-binding protein essential for its stability, pseudouridylation, and correct biogenesis of the mitochondrial large ribosomal subunit...
April 3, 2018: Cell Reports
https://www.readbyqxmd.com/read/29615055/differential-susceptibilities-of-anopheles-albimanus-and-anopheles-stephensi-mosquitoes-to-ivermectin
#13
Staci M Dreyer, Kelsey J Morin, Jefferson A Vaughan
BACKGROUND: Vector control is a crucial element of anti-malaria campaigns and works best when there is a thorough knowledge of the biology and behaviour of the Anopheles vector species responsible for transmitting malaria within a given locale. With the push to eradicate malaria stronger than ever, there is a growing need to develop and deploy control strategies that exploit the behavioural attributes of local vector species. This is especially true in regions where the vectors are exophagic (i...
April 3, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29614102/does-growth-path-influence-beef-lipid-deposition-and-fatty-acid-composition
#14
Ana S H Costa, Paulo Costa, Susana P Alves, Cristina M Alfaia, José A M Prates, Veronica Vleck, Isabelle Cassar-Malek, Jean-François Hocquette, Rui J B Bessa
Despite the recent advances in transcriptomics, gene expression studies addressing cattle´s skeletal muscle adaptations in response to compensatory growth are warranted, particularly regarding lipid metabolism due to its impact in meat sensory and nutritional traits. In the present study, in comparison to ad libitum feeding, a period of feed restriction was used in order to understand the changes in bull´s lipid metabolism and gene expression of the adipogenic and lipogenic pathways after re-alimentation...
2018: PloS One
https://www.readbyqxmd.com/read/29596928/cardiac-safety-evaluation-in-zebrafish-and-in-silico-adme-prediction-of-cephalosporins-with-an-aminothiazoyl-ring-at-the-c-7-position
#15
Ying Han, Bo Chen, Jingpu Zhang, Changqin Hu
Systems toxicology approaches have been used as important tools in the drug discovery and medicine quality control processes. The aim of this study was to assess the pharmacokinetic and toxicity properties of cephalosporins with an aminothiazoyl ring at the C-7 position (CATRs). Cardiac toxicity of the compounds was assessed in zebrafish embryos, and it was determined that CATRs disturbed the formation and development of the heart in a dose-dependent manner. Differentially expressed genes (DEGs) related to the heart were also identified by transcriptome analysis, and co-DEGs were obtained in the protein-protein interaction (PPI) network...
March 26, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29596388/genetic-variants-in-stat3-promoter-regions-and-their-application-in-molecular-breeding-for-body-size-traits-in-qinchuan-cattle
#16
Sen Wu, Yaning Wang, Yue Ning, Hongfang Guo, Xiaoyu Wang, Le Zhang, Rajwali Khan, Gong Cheng, Hongbao Wang, Linsen Zan
Signal transducer and activator of transcription 3 (STAT3) plays a critical role in leptin-mediated regulation of energy metabolism. This study investigated genetic variation in STAT3 promoter regions and verified their contribution to bovine body size traits. We first estimated the degree of conservation in STAT3, followed by measurements of its mRNA expression during fetal and adult stages of Qinchuan cattle. We then sequenced the STAT3 promoter region to determine genetic variants and evaluate their association with body size traits...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29593177/molecular-pathogenesis-of-familial-wolff-parkinson-white-syndrome
#17
Miyamotoa Licht
Familial Wolff-Parkinson-White (WPW) syndrome is an autosomal dominant inherited disease and consists of a small percentage of WPW syndrome which exhibits ventricular pre-excitation by development of accessory atrioventricular pathway. A series of mutations in PRKAG2 gene encoding gamma2 subunit of 5'AMP-activated protein kinase (AMPK) has been identified as the cause of familial WPW syndrome. AMPK is one of the most important metabolic regulators of carbohydrates and lipids in many types of tissues including cardiac and skeletal muscles...
2018: Journal of Medical Investigation: JMI
https://www.readbyqxmd.com/read/29581852/rhoa-rho-kinase-triggers-epithelial-mesenchymal-transition-in-mesothelial-cells-and-contributes-to-the-pathogenesis-of-dialysis-related-peritoneal-fibrosis
#18
Qinglian Wang, Xiaowei Yang, Ying Xu, Zhenwei Shen, Hongxia Cheng, Fajuan Cheng, Xiang Liu, Rong Wang
Peritoneal fibrosis (PF) with associated peritoneal dysfunction is almost invariably observed in long-term peritoneal dialysis (PD) patients. Advanced glycation end products (AGEs) are pro-oxidant compounds produced in excess during the metabolism of glucose and are present in high levels in standard PD solutions. The GTPase RhoA has been implicated in PF, but its specific role remains poorly understood. Here, we studied the effects of RhoA/Rho-kinase signaling in AGEs-induced epithelial-mesenchymal transition (EMT) in human peritoneal mesothelial cells (HPMCs), and evaluated morphological and molecular changes in a rat model of PD-related PF...
March 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29578204/differences-in-gene-expression-related-to-the-results-of-obesity-treatment-peak-oxygen-uptake-and-fatty-acid-metabolism-measured-in-a-cardiopulmonary-exercise-test
#19
Marta Gruchała-Niedoszytko, Pieter van der Vlies, Piotr Niedoszytko, Bahram Sanjabi, Marek Niedoszytko, Małgorzata Kaczkan, Magdalena Pieszko, Katarzyna Gierat-Haponiuk, Aleksandra Śliwińska, Dominika Szalewska, Sylwia Małgorzewicz
INTRODUCTION    The impact of obesity requires methods used on a large scale as  cardiopulmonary exercise test (CPET). The gene expression may explain CPET results on molecular level.   OBJECTIVES    The aim of the study was to compare gene expression in obesity,  differences related to the CPET results. PATIENTS AND METHODS    The study group consisted of 9 obese and 7 controls. The treatment consisted of diet, rehabilitation, behavioural therapy. Diet was based on the body composition analysed by bioelectrical impedance (BIA), resting metabolic rate, and subjective patients' preferences...
March 26, 2018: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/29577999/acute-stress-alters-the-rates-of-degradation-of-cardiac-muscle-proteins
#20
Bethany Geary, Kieran Magee, Phillip Cash, Holger Husi, Iain S Young, Phillip D Whitfield, Mary K Doherty
Stressful experiences can have detrimental effects on many aspects of health and wellbeing. The zebrafish (Danio rerio) is a widely used model for stress research and a stress phenotype can be induced by manipulating the environmental conditions and social interactions. In this study we have combined a zebrafish stress model with the measurement of degradation rates of soluble cardiac muscle proteins. The results showed that the greater the stress response in the zebrafish the lower the level of overall protein degradation...
March 22, 2018: Journal of Proteomics
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