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https://www.readbyqxmd.com/read/27935984/a-biodegradable-polyethylenimine-based-vector-modified-by-trifunctional-peptide-r18-for-enhancing-gene-transfection-efficiency-in-vivo
#1
Jing Hu, Manman Zhu, Kehai Liu, Hua Fan, Wenfang Zhao, Yuan Mao, Yaguang Zhang
Lack of capacity to cross the nucleus membrane seems to be one of the main reasons for the lower transfection efficiency of gene vectors observed in vivo study than in vitro. To solve this problem, a new non-viral gene vector was designed. First, a degradable polyethylenimine (PEI) derivate was synthesized by crosslinking low-molecular-weight (LMW) PEI with N-octyl-N-quaternary chitosan (OTMCS), and then adopting a designed trifunctional peptide (RGDC-TAT-NLS) with good tumor targeting, cell uptake and nucleus transport capabilities to modify OTMCS-PEI...
2016: PloS One
https://www.readbyqxmd.com/read/27935164/kaolin-activated-thromboelastography-and-standard-coagulation-assays-in-cyanotic-and-acyanotic-infants-undergoing-complex-cardiac-surgery-a-prospective-cohort-study
#2
Alessandra Rizza, Zaccaria Ricci, Chiara Pezzella, Isabella Favia, Giovina Di Felice, Marco Ranucci, Paola Cogo
BACKGROUND AND AIMS: Several studies report the use of thromboelatography (TEG) to monitor coagulation in pediatric cardiac surgery. The aim of this study was to compare baseline and intraoperative TEG, TEG-functional fibrinogen, and standard coagulation assays in children with cyanotic and acyanotic congenital heart disease (CHD) undergoing cardiac surgery. METHODS: This is a prospective observational study of 63 children aged <24 months undergoing cardiac surgery with cardiopulmonary bypass (CPB)...
December 9, 2016: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/27933968/emissive-h-aggregates-of-an-ultrafast-molecular-rotor-a-promising-platform-for-sensing-heparin
#3
Niyati H Mudliar, Prabhat K Singh
Constructing "turn on" fluorescent probes for heparin, a most widely used anticoagulant in clinics, from commercially available materials is of great importance, but remains challenging. Here, we report the formation of a rarely observed emissive H-aggregate of an ultrafast molecular rotor dye, Thioflavin-T, in the presence of heparin, which provides an excellent platform for simple, economic and rapid fluorescence turn-on sensing of heparin. Generally, H-aggregates are considered as serious problem in the field of biomolecular sensing, owing to their poorly emissive nature resulting from excitonic interaction...
November 23, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27933952/end-site-specific-conjugation-of-enoxaparin-and-tetradeoxycholic-acid-using-nonenzymatic-glycosylation-for-oral-delivery
#4
Jooho Park, Ok Cheol Jeon, Jisuk Yun, Hwajung Nam, Jinha Hwang, Taslim A Al-Hilal, Kwangmeyung Kim, Kyungjin Kim, Youngro Byun
Heparin and low molecular weight heparins (LMWHs) have been the drug of choice for the treatment or the prevention of thromboembolic disease. Different methods are employed to prepare the LMWHs that are clinically approved for the market currently. In particular, enoxaparin, which has a reducing sugar moiety at the end-site of polysaccharide, is prepared by alkaline depolymerization. Focusing on this end-site-specific activity of LMWHs, we conjugated the tetraoligomer of deoxycholic acid (TetraDOCA; TD) at the end-site of enoxaparin via nonenzymatic glycosylation reaction...
December 8, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27933395/influence-of-the-her-receptor-ligand-system-on-sensitivity-to-cetuximab-and-trastuzumab-in-gastric-cancer-cell-lines
#5
Julia Kneissl, Anja Hartmann, Nicole Pfarr, Franziska Erlmeier, Thomas Lorber, Simone Keller, Gwen Zwingenberger, Wilko Weichert, Birgit Luber
PURPOSE: Gastric cancer remains a major health concern, and improvement of the therapeutic options is crucial. Treatment with targeted therapeutics such as the EGFR-targeting antibody cetuximab or the HER2-targeting antibody trastuzumab is either ineffective or moderately effective in this disease, respectively. In this study, we analysed the involvement of the HER receptor ligands amphiregulin (AREG), epidermal growth factor (EGF), heparin-binding epidermal growth factor (HB-EGF) and transforming growth factor alpha (TGFα) in the responsiveness of gastric cancer cell lines to cetuximab and trastuzumab...
December 8, 2016: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/27933303/purification-of-baculovirus-vectors-using-heparin-affinity-chromatography
#6
Md Nasimuzzaman, Danielle Lynn, Johannes Cm van der Loo, Punam Malik
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do not elicit strong immune or allergic responses in vivo. Hence, their use as gene delivery vehicles has become increasingly popular in recent years. Moreover, baculovirus vectors carrying mammalian regulatory elements can efficiently transduce and express transgenes in mammalian cells...
2016: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/27932155/late-allograft-renal-vein-thrombosis-treated-with-anticoagulation-alone-a-case-report
#7
C Ferreira, L Pereira, P Pereira, I Tavares, S Sampaio, M Bustorff, M Pestana
BACKGROUND: Allograft renal vein thrombosis is a rare complication of kidney transplantation. Most cases occur in the first 2 weeks after transplantation, but there are cases described many years after the transplant surgery. Allograft loss is the usual outcome. METHODS: We present a case of a renal transplant recipient with allograft renal vein thrombosis associated with deep venous thrombosis of a lower limb, 9 years after transplantation. He was successfully treated with anticoagulation alone, with recovery of allograft function...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27931550/off-pump-coronary-artery-bypass-grafting-using-continuous-heparin-infusion
#8
Kamales Kumar Saha, Dinesh Kumar Sangwan, Pravin K Shroff, Mandar Manohar Deval, Kakalee K Saha, Rinu V Jacob, Ratnaprobha Adsul, Lukash Jagdale
OBJECTIVES: Levels of anticoagulation during off-pump coronary artery bypass grafting (OPCAB) remain controversial. Prolonged activated clotting time (ACT) during OPCAB increases blood loss during surgery and can also cause paradoxical increase in postoperative myocardial infarction. Shorter ACT can increase thrombotic complication. Maintaining a steady ACT level is challenging. We have used continuous heparin infusion after initial bolus during OPCAB to maintain a steady low target ACT...
November 2016: Indian Heart Journal
https://www.readbyqxmd.com/read/27928742/implementation-of-infrared-and-raman-modalities-for-glycosaminoglycan-characterization-in-complex-systems
#9
Hossam Taha Mohamed, Valérie Untereiner, Ganesh D Sockalingum, Stéphane Brézillon
Glycosaminoglycans (GAGs) are natural, linear and negatively charged heteropolysaccharides which are incident in every mammalian tissue. They consist of repeating disaccharide units, which are composed of either sulfated or non-sulfated monosaccharides. Depending on tissue types, GAGs exhibit structural heterogeneity such as the position and degree of sulfation or within their disaccharide units composition being heparin, heparan sulfate, chondroitine sulfate, dermatan sulfate, keratan sulfate, and hyaluronic acid...
December 7, 2016: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27927497/acute-renal-infarction-after-lateral-lumbar-interbody-spinal-fusion-for%C3%A2-kyphoscoliosis
#10
Sanjeev J Suratwala, MaryAnne Cronin, Katelyn Kondra, Vincent Leone
STUDY DESIGN: Case report. OBJECTIVES: Case report of acute renal infarction following lateral interbody fusion with posterior instrumentation for adult lumbar kyphoscoliosis. SUMMARY OF BACKGROUND DATA: This is the first reported renal infarction following minimally invasive lateral interbody fusion and posterior spinal instrumentation. METHODS: We report a case of acute renal infarction in a 72-year-old woman following direct lateral interbody fusion (DLIF) with percutaneous pedicle screw and rod fixation from L1 to L5 for correction of kyphoscoliosis...
September 2016: Spine Deformity
https://www.readbyqxmd.com/read/27925225/preclinical-study-of-anticoagulation-regimens-in-sheep-after-implantation-of-ch-vad-blood-pump
#11
Chuangye Xu, Guanghui Wu, Xiujian Liu, Yuna He, Xiaotong Hou, Haiyang Li, Chen Chen, Peng Yang, Changyan Lin
Effective anticoagulation regimens are needed to reduce risks of thrombosis and bleeding in animal models of ventricular assist device to verify its hemocompatibility, biologic safety and reliability. This study is to develop a validated anticoagulation procedure for a sheep model to test the newly developed CH-VAD. CH-VAD models were established in six healthy sheep by constructing blood bypass of left ventricle → ventricular assist device → descending aorta. Heparin infusion was used during operation and in the prior 4 days to maintain activated clotting time 1...
December 7, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27924343/synthesis-and-formulation-of-functional-bionanomaterials-with-superoxide-dismutase-activity
#12
Marko Pavlovic, Paul Rouster, Istvan Szilagyi
Layered double hydroxide (LDH) nanoparticles were prepared and used as solid support for superoxide dismutase (SOD) enzymes. Structural features were studied by XRD, spectroscopic methods (IR, UV-Vis and fluorescence) and TEM, while colloidal stability of the obtained materials was investigated by electrophoresis and light scattering in aqueous dispersions. The SOD quantitatively adsorbed on the LDH by electrostatic and hydrophobic interactions and kept its structural integrity upon immobilization. The composite material showed moderate resistance against salt-induced aggregation in dispersions, therefore, heparin polyelectrolyte was used to improve the colloidal stability of the system...
December 7, 2016: Nanoscale
https://www.readbyqxmd.com/read/27923924/attenuation-of-the-type-iv-pilus-retraction-motor-influences-neisseria-gonorrhoeae-social-and-infection-behavior
#13
Alyson M Hockenberry, Danielle M Hutchens, Al Agellon, Magdalene So
: Retraction of the type IV pilus (Tfp) mediates DNA uptake, motility, and social and infection behavior in a wide variety of prokaryotes. To date, investigations into Tfp retraction-dependent activities have used a mutant deleted of PilT, the ATPase motor protein that causes the pilus fiber to retract. ΔpilT cells are nontransformable, nonmotile, and cannot aggregate into microcolonies. We tested the hypothesis that these retraction-dependent activities are sensitive to the strength of PilT enzymatic activity by using the pathogen Neisseria gonorrhoeae as a model...
December 6, 2016: MBio
https://www.readbyqxmd.com/read/27923784/low-molecular-weight-heparin-does-not-prevent-vte-after-knee-arthroscopy-studies-show
#14
Susan Mayor
No abstract text is available yet for this article.
December 5, 2016: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/27923477/hydrophilic-interaction-chromatography-multiple-reaction-monitoring-mass-spectrometry-method-for-basic-building-block-analysis-of-low-molecular-weight-heparins-prepared-through-nitrous-acid-depolymerization
#15
Xiaojun Sun, Zhimou Guo, Mengqi Yu, Chao Lin, Anran Sheng, Zhiyu Wang, Robert J Linhardt, Lianli Chi
Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin. Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications. Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction. Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs...
November 30, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27922880/investigation-of-key-circuit-constituents-affecting-drug-sequestration-during-extracorporeal-membrane-oxygenation-treatment
#16
Jiheum Park, Dong Ah Shin, Saram Lee, Young-Jae Cho, Sanghoon Jheon, Jung Chan Lee, Hee Chan Kim
We quantified the influence of the elements of the extracorporeal oxygenation (ECMO) circuit on drug sequestration by focusing on the interactions between materials and drugs. Tubing of three different brands (Tygon/Maquet/Terumo) and oxygenators of two different brands (Maquet/Terumo) were used. Drugs included dexmedetomidine, meropenem, and heparin which were dissolved in deionized water. Tubing was cut into ~7 cm sections and allowed drug solutions enclosed inside by clamping both ends. The oxygenator housing, gas membrane, and heat exchanger were dissected into ~1 g pieces and submerged into drug solutions...
December 2, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/27922061/thermo-sensitive-hydrogels-combined-with-decellularised-matrix-deliver-bfgf-for-the-functional-recovery-of-rats-after-a-spinal-cord-injury
#17
He-Lin Xu, Fu-Rong Tian, Cui-Tao Lu, Jie Xu, Zi-Liang Fan, Jing-Jing Yang, Pian-Pian Chen, Ya-Dong Huang, Jian Xiao, Ying-Zheng Zhao
Because of the short half-life, either systemic or local administration of bFGF shows significant drawbacks to spinal injury. In this study, an acellular spinal cord scaffold (ASC) was encapsulated in a thermo-sensitive hydrogel to overcome these limitations. The ASC was firstly prepared from the spinal cord of healthy rats and characterized by scanning electronic microscopy and immunohistochemical staining. bFGF could specifically complex with the ASC scaffold via electrostatic or receptor-mediated interactions...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921101/the-effect-of-common-anticoagulants-in-detection-and-quantification-of-malaria-parasitemia-in-human-red-blood-cells-by-atr-ftir-spectroscopy
#18
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has the potential to become a new diagnostic tool for malaria and other diseases. For point-of-care testing, the use of ATR-FTIR in malaria diagnosis enables the analysis of blood in the aqueous state, which represents an enormous advantage by minimising the sample preparation by removing the need for cell fixation. Here we report the quantification of malaria parasitemia in human RBCs in their normal physiological aqueous state...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27920522/incorporation-of-heparin-binding-proteins-into-preformed-dextran-sulfate-chitosan-nanoparticles
#19
Paula Zaman, Julia Wang, Adam Blau, Weiping Wang, Tina Li, Daniel S Kohane, Joseph Loscalzo, Ying-Yi Zhang
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27920020/repeated-failed-non-invasive-prenatal-testing-in-a-woman-with-immune-thrombocytopenia-and-antiphospholipid-syndrome-lessons-learnt
#20
C Y Y Hui, W C Tan, E L Tan, L K Tan
We present a case of a 37-year-old Chinese woman (gravida 4 para 0) with a history of immune thrombocytopenia and type IIb antiphospholipid syndrome. She was started on 100 mg of aspirin, 20 mg of prednisolone and 20 mg of subcutaneous low-molecular-weight heparin daily for her fourth pregnancy. She opted for non-invasive prenatal testing for aneuploidy screening but had failed results three times consecutively from insufficient fetal cfDNA initially or high variance in cfDNA counts on redraws. She declined invasive karyotyping...
December 5, 2016: BMJ Case Reports
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