keyword
https://read.qxmd.com/read/38487542/directing-the-migration-of-serum-free-ex-vivo-expanded-v%C3%AE-9v%C3%AE-2-t-cells
#1
JOURNAL ARTICLE
Kiran K Parwani, Gianna M Branella, Rebecca E Burnham, Andre J Burnham, Austre Y Schiaffino Bustamante, Elisabetta Manuela Foppiani, Kristopher A Knight, Brian G Petrich, Edwin M Horwitz, Christopher B Doering, H Trent Spencer
Vγ9Vδ2 T cells represent a promising cancer therapy platform because the implementation of allogenic, off-the-shelf product candidates is possible. However, intravenous administration of human Vγ9Vδ2 T cells manufactured under good manufacturing practice (GMP)-compliant, serum-free conditions are not tested easily in most mouse models, mainly because they lack the ability to migrate from the blood to tissues or tumors. We demonstrate that these T cells do not migrate from the circulation to the mouse bone marrow (BM), the site of many malignancies...
2024: Frontiers in Immunology
https://read.qxmd.com/read/37239354/evaluating-the-usefulness-of-human-dna-quantification-to-predict-dna-profiling-success-of-historical-bone-samples
#2
JOURNAL ARTICLE
Jacqueline Tyler Thomas, Courtney Cavagnino, Katelyn Kjelland, Elise Anderson, Kimberly Sturk-Andreaggi, Jennifer Daniels-Higginbotham, Christina Amory, Brian Spatola, Kimberlee Moran, Walther Parson, Charla Marshall
This study assessed the usefulness of DNA quantification to predict the success of historical samples when analyzing SNPs, mtDNA, and STR targets. Thirty burials from six historical contexts were utilized, ranging in age from 80 to 800 years postmortem. Samples underwent library preparation and hybridization capture with two bait panels (FORCE and mitogenome), and STR typing (autosomal STR and Y-STR). All 30 samples generated small (~80 bp) autosomal DNA target qPCR results, despite mean mappable fragments ranging from 55-125 bp...
April 27, 2023: Genes
https://read.qxmd.com/read/36191412/allele-frequencies-of-31-autosomal-short-tandem-repeat-austr-loci-obtained-using-the-precision-id-globalfiler%C3%A2-ngs-str-panel-v2-in-322-individuals-from-the-japanese-population
#3
JOURNAL ARTICLE
Tsukasa Ohuchi, Xueting Guan, Eriko Hirai, Masaki Hashiyada, Sho Manabe, Atsushi Akane, Noboru Adachi, Keiji Tamaki, Masato Funayama
In human identification methods that target short tandem repeats (STRs), massively parallel sequencing (MPS) technology has made it possible to genotype at the level of the specific sequence itself. This allows for the detection of repeat unit variants and single nucleotide polymorphisms (SNPs) adjacent to the STRs. Using the GlobalFiler™ NGS STR Panel v2, Ion S5, and Converge software, this study constructed a Japanese database of 31 autosomal STRs (auSTRs) and two sex markers from 322 individuals. After excluding some sequence errors and stutters, a total of 31 novel alleles were identified...
September 27, 2022: Legal Medicine
https://read.qxmd.com/read/35819898/-pedobacter-mucosus-sp-nov-isolated-from-a-soil-sample-of-glacier-foreland-in-austre-lov%C3%A3-nbreen-arctic
#4
JOURNAL ARTICLE
Jun Huang, Xiaoya Peng, Kun Qin, Yixuan Liu, Jingjing Niu, Jia Liu, Jielin Dong, Yongping Zhang, Fang Peng
A rod-shaped, Gram-stain-negative, non-motile and aerobic bacterium, designated Q8-18T , was isolated from soil of glacier foreland in Austre Lovénbreen, Arctic, and subjected to a polyphasic taxonomic study. Strain Q8-18T grew optimally at 20 °C, pH 5.0-8.0 and in the presence of 0-1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q8-18T belonged to the family Sphingobacteriaceae . Strain Q8-18T showed the highest sequence similarity to Pedobacter mendelii CCM 8685T (96...
July 2022: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/34940290/cytotoxic-tumour-selective-1-5-diaryl-3-oxo-1-4-pentadienes-mounted-on-a-piperidine-ring
#5
JOURNAL ARTICLE
Praveen K Roayapalley, Hiroshi Sakagami, Keitaro Satoh, Shigeru Amano, Kenjiro Bandow, Renato J Aguilera, Karla G Cano Hernandez, Austre Y Schiaffino Bustamante, Stephen G Dimmock, Rajendra K Sharma, Umashankar Das, Jonathan R Dimmock
A series of 3,5-bis(benzylidene)-4-piperidones 2a - u were prepared as candidate cytotoxic agents. In general, the compounds are highly toxic to human gingival carcinoma (Ca9-22), human squamous carcinoma-2 (HSC-2) and human squamous carcinoma-4 (HSC-4) neoplasms, but less so towards non-malignant human gingival fibroblast (HGF), human periodontal ligament fibroblast (HPLF) and human pulp cells (HPC), thereby demonstrating tumour-selective toxicity. A further study revealed that most of the compounds in series 2 were more toxic to the human Colo-205 adenocarcinoma cell line (Colo-205), human HT29 colorectal adenocarcinoma cells (HT-29) and human CEM lymphoid cells (CEM) neoplasms than towards non-malignant human foreskin Hs27 fibroblast line (Hs27) cells...
December 16, 2021: Medicines (Basel, Switzerland)
https://read.qxmd.com/read/34448104/three-novel-piperidones-exhibit-tumor-selective-cytotoxicity-on-leukemia-cells-via-protein-degradation-and-stress-mediated-mechanisms
#6
JOURNAL ARTICLE
Lisett Contreras, Stephanie Medina, Austre Y Schiaffino Bustamante, Edgar A Borrego, Carlos A Valenzuela, Umashankar Das, Subhas S Karki, Jonathan R Dimmock, Renato J Aguilera
BACKGROUND: Cancer is an ongoing worldwide health problem. Although chemotherapy remains the mainstay therapy for cancer, it is not always effective and has detrimental side effects. Here, we present piperidone compounds P3, P4, and P5 that selectively target cancer cells via protein- and stress-mediated mechanisms. METHODS: We assessed typical apoptotic markers including phosphatidylserine externalization, caspase-3 activation, and DNA fragmentation through flow cytometry...
August 26, 2021: Pharmacological Reports: PR
https://read.qxmd.com/read/33432410/spatial-analysis-of-supraglacial-debris-cover-in-svalbard-arctic-region-a-decadal-study
#7
JOURNAL ARTICLE
Geetha Priya Murugesan, Varshini Narayan, Suresh Devaraj
Continental glacier melts directly influence the environment, resulting in sea-level rise affecting the settlements along the coast. The increase in global warming and constant change in the glacier mass grabbed the attention of researchers in understanding the evolution and distribution of glaciers. Despite the increase in the number of glacier studies, the difficulty is experienced by the researchers in understanding the supra-glacial debris cover and its characteristics. Supraglacial debris cover affects surface melt by increasing and decreasing ablation under thin and thick debris cover...
May 2021: Environmental Science and Pollution Research International
https://read.qxmd.com/read/33274824/synthesis-molecular-docking-and-preliminary-antileukemic-activity-of-4-methoxybenzyl-derivatives-bearing-an-imidazo-2-1-b-1-3-4-thiadiazoles
#8
JOURNAL ARTICLE
Subhas S Karki, B Choodamani, Karla G Cano Hernandez, Sujeet Kumar, Anna M Tony, Austre Y Schiaffino Bustamante, Renato J Aguilera, Dominique Schols, GopiMohan Gopi C
In this study, we synthesized 22 compounds in a series with various substitution on imidazo[2,1- b ][1,3,4]thiadiazoles. The potential cytotoxic activity of these compounds investigated in leukemia cell lines by Differential Nuclear Staining (DNS). Our results identify two compounds 2-(4-methoxybenzyl)-6-(2-oxo-2H-chromen-3-yl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl thiocyanate and 6-(4-chlorophenyl)-2-(4'-methoxybenzyl)imidazo[2,1-b][1,3,4]thiadiazole-5-carbaldehyde exhibited as the most cytotoxic effect against murine leukemia cells (L1210), human T-lymphocyte cells (CEM) and human cervix carcinoma cells (HeLa) with IC 50 values ranging between 0...
December 4, 2020: Chemistry & Biodiversity
https://read.qxmd.com/read/32892077/source-timing-and-dynamics-of-ionic-species-mobility-in-the-svalbard-annual-snowpack
#9
JOURNAL ARTICLE
Andrea Spolaor, Cristiano Varin, Xanthi Pedeli, Jean Marc Christille, Torben Kirchgeorg, Fabio Giardi, David Cappelletti, Clara Turetta, Warren R L Cairns, Andrea Gambaro, Andrea Bernagozzi, Jean Charles Gallet, Mats P Björkman, Elena Barbaro
Nearly all ice core archives from the Arctic and middle latitudes (such as the Alps), apart from some very high elevation sites in Greenland and the North Pacific, are strongly influenced by melting processes. The increases in the average Arctic temperature has enhanced surface snow melting even of higher elevation ice caps, especially on the Svalbard Archipelago. The increase of the frequency and altitude of winter "rain on snow" events as well as the increase of the length of the melting season have had a direct impact on the chemical composition of the seasonal and permanent snow layers due to different migration processes of water-soluble species, such as inorganic ions...
August 18, 2020: Science of the Total Environment
https://read.qxmd.com/read/31252251/massively-parallel-sequence-data-of-31-autosomal-str-loci-from-496-spanish-individuals-revealed-concordance-with-ce-str-technology-and-enhanced-discrimination-power
#10
JOURNAL ARTICLE
Pedro A Barrio, Pablo Martín, Antonio Alonso, Petra Müller, Martin Bodner, Burkhard Berger, Walther Parson, Bruce Budowle
This study reports Short Tandem Repeat (STR) sequence-based allele data from 496 Spanish individuals across 31 autosomal STR (auSTR) loci included in the Precision ID GlobalFiler™ NGS STR Panel v2: D12S391, D13S317, D8S1179, D21S11, D3S1358, D5S818, D1S1656, D2S1338, vWA, D2S441, D5S2800, D7S820, D16S539, D6S474, D12ATA63, D4S2408, D6S1043, D19S433, D14S1434, CSF1PO, D10S1248, D18S51, D1S1677, D22S1045, D2S1776, D3S4529, FGA, Penta D, Penta E, TH01 and TPOX. The sequence of each allele was aligned to the reference sequence GRCh37 (hg19) and formatted according to the guidance of the International Society for Forensic Genetics...
June 14, 2019: Forensic Science International. Genetics
https://read.qxmd.com/read/30061079/fragrances-and-pahs-in-snow-and-seawater-of-ny-%C3%A3-lesund-svalbard-local-and-long-range-contamination
#11
JOURNAL ARTICLE
Marco Vecchiato, Elena Barbaro, Andrea Spolaor, Francois Burgay, Carlo Barbante, Rossano Piazza, Andrea Gambaro
Polar regions are fragile ecosystems threatened by both long-range pollution and local human contamination. In this context, the environmental distribution of the Personal Care Products (PCPs) represent a major knowledge gap. Following preliminary Antarctic studies, Fragrance Materials (FMs) were analyzed in the seawater and snow collected in the area of Ny-Ålesund, Svalbard, to investigate local and long-range contamination. Polycyclic Aromatic Hydrocarbons (PAHs), including Retene, were determined in parallel to help the identification of the governing processes...
November 2018: Environmental Pollution
https://read.qxmd.com/read/26827927/psychrobacter-glaciei-sp-nov-isolated-from-the-ice-core-of-an-arctic-glacier
#12
JOURNAL ARTICLE
Yin-Xin Zeng, Yong Yu, Yang Liu, Hui-Rong Li
A Gram-stain-negative, non-motile, non-spore-forming, non-pigmented, oxidase- and catalase-positive bacterial strain, designated BIc20019T, was isolated from the ice core of Austre Lovénbreen in Ny-Ålesund, Svalbard. The temperature and NaCl ranges for growth were 4-34 °C (optimum, 25-29 °C) and 0-8% (w/v) (optimum, 2-4%). Analysis of the 16S rRNA gene sequence indicated that strain BIc20019T belonged to the genus Psychrobacter and was closely related to Psychrobacter arcticus 273-4T, Psychrobacter cryohalolentis K5T, 'Psychrobacter fjordensis' BSw21516B, Psychrobacter fozii LMG 21280T, Psychrobacter luti LMG 21276T and Pyschrobacter okhotskensis MD17T at greater than 99% similarity...
April 2016: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/24763603/pedobacter-huanghensis-sp-nov-and-pedobacter-glacialis-sp-nov-isolated-from-arctic-glacier-foreland
#13
JOURNAL ARTICLE
Xia Qiu, Zhihao Qu, Fan Jiang, Lvzhi Ren, Xulu Chang, Wenjing Kan, Chengxiang Fang, Fang Peng
Two psychrotolerant, Gram-stain-negative, rod-shaped bacterial strains, designed M1-27(T) and 8-24(T), were subjected to polyphasic taxonomic studies. Strain M1-27(T) was isolated from the foreland of the Midtre Lovénbreen glacier, whereas strain 8-24(T) was isolated from the foreland of the Austre Lovénbreen glacier. Both were Arctic glacier forelands, near Ny-Ålesund, Svalbard Archipelago, Norway. Strains M1-27(T) and 8-24(T) exhibited 16S rRNA gene sequence similarities of 91.0-96.0% and 92.3-96.7%, respectively, to type strains of recognized species of the genus Pedobacter...
July 2014: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/24346230/diversity-cold-active-enzymes-and-adaptation-strategies-of-bacteria-inhabiting-glacier-cryoconite-holes-of-high-arctic
#14
JOURNAL ARTICLE
Purnima Singh, Shiv M Singh, Prashant Dhakephalkar
Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic bacterial diversity from cryoconite holes at Midre Lovénbreen (ML), Austre Brøggerbreen (AB), and Vestre Brøggerbreen (VB) glaciers. The culturable bacterial count ranged from 2.7 × 10(3) to 8.8 × 10(4) CFUs/g while the total bacterial numbers ranged from 5.07 × 10(5) to 1.50 × 10(6) cells at the three glaciers. A total of 35 morphologically distinct bacterial isolates were isolated...
March 2014: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/23474777/diversity-of-bacteria-in-surface-ice-of-austre-lov%C3%A3-nbreen-glacier-svalbard
#15
JOURNAL ARTICLE
Yin-Xin Zeng, Ming Yan, Yong Yu, Hui-Rong Li, Jian-Feng He, Kun Sun, Fang Zhang
Two 16S rRNA gene clone libraries Cores 1U and 2U were constructed using two ice core samples collected from Austre Lovénbreen glacier in Svalbard. The two libraries yielded a total of 262 clones belonging to 59 phylotypes. Sequences fell into 10 major lineages of the domain Bacteria, including Proteobacteria (alpha, beta, gamma and delta subdivisions), Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Deinococcus-Thermus, Chloroflexi, Planctomycetes, Cyanobacteria and candidate division TM7. Among them, Bacteroidetes, Actinobacteria, Alphaproteobacteria and Cyanobacteria were most abundant...
May 2013: Archives of Microbiology
https://read.qxmd.com/read/20664552/possible-interactions-between-bacterial-diversity-microbial-activity-and-supraglacial-hydrology-of-cryoconite-holes-in-svalbard
#16
JOURNAL ARTICLE
Arwyn Edwards, Alexandre M Anesio, Sara M Rassner, Birgit Sattler, Bryn Hubbard, William T Perkins, Michael Young, Gareth W Griffith
The diversity of highly active bacterial communities in cryoconite holes on three Arctic glaciers in Svalbard was investigated using terminal restriction fragment length polymorphism (T-RFLP) of the 16S rRNA locus. Construction and sequencing of clone libraries allowed several members of these communities to be identified, with Proteobacteria being the dominant one, followed by Cyanobacteria and Bacteroidetes. T-RFLP data revealed significantly different communities in holes on the (cold) valley glacier Austre Brøggerbreen relative to two adjacent (polythermal) valley glaciers, Midtre Lovénbreen and Vestre Brøggerbreen...
January 2011: ISME Journal
https://read.qxmd.com/read/15578570/head-structures-of-males-of-strepsiptera-hexapoda-with-emphasis-on-basal-splitting-events-within-the-order
#17
JOURNAL ARTICLE
Rolf Georg Beutel, Hans Pohl
Internal and external head structures of males of Strepsiptera were examined and the head of a species of Mengenilla is described in detail. The results suggest a reinterpretation of some structures. The head of basal extant strepsipterans is subprognathous, whereas it is strictly orthognathous in the groundplan of Strepsiptera s.l. The labrum and hypopharynx are not part of the mouthfield sclerite. The labial palps are absent in all strepsipterans. A very slightly modified mandibular articulation is preserved in Eoxenos, whereas it is distinctly reduced in other extant groups...
May 2006: Journal of Morphology
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