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https://www.readbyqxmd.com/read/28230188/3d-time-lapse-imaging-and-quantification-of-mitochondrial-dynamics
#1
Miguel Sison, Sabyasachi Chakrabortty, Jérôme Extermann, Amir Nahas, Paul James Marchand, Antonio Lopez, Tanja Weil, Theo Lasser
We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake and mitochondria targeting efficiency. The high stability of the gold nanoparticles allows continuous imaging over an extended time up to 3000 seconds without significant cell damage...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28228173/optimization-of-vascular-casting-for-three-dimensional-fluorescence-cryo-imaging-of-collateral-vessels-in-the-ischemic-rat-hindlimb
#2
Janina C V Schwarz, Monique G J T B van Lier, Erik N T P Bakker, Judith de Vos, Jos A E Spaan, Ed VanBavel, Maria Siebes
Development of collateral vessels, arteriogenesis, may protect against tissue ischemia, however, quantitative data on this process remain scarce. We have developed a technique for replicating the entire arterial network of ischemic rat hindlimbs in three dimensions (3D) based on vascular casting and automated sequential cryo-imaging. Various dilutions of Batson's No. 17 with methyl methacrylate were evaluated in healthy rats, with further protocol optimization in ischemic rats. Penetration of the resin into the vascular network greatly depended on dilution; the total length of casted vessels below 75 µm was 13-fold higher at 50% dilution compared with the 10% dilution...
February 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28222869/accuracy-evaluation-of-intraoral-optical-impressions-a-clinical-study-using-a-reference-appliance
#3
Mohammad A Atieh, André V Ritter, Ching-Chang Ko, Ibrahim Duqum
STATEMENT OF PROBLEM: Trueness and precision are used to evaluate the accuracy of intraoral optical impressions. Although the in vivo precision of intraoral optical impressions has been reported, in vivo trueness has not been evaluated because of limitations in the available protocols. PURPOSE: The purpose of this clinical study was to compare the accuracy (trueness and precision) of optical and conventional impressions by using a novel study design. MATERIAL AND METHODS: Five study participants consented and were enrolled...
February 17, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28219649/a-novel-approach-to-segmentation-and-measurement-of-medical-image-using-level-set-methods
#4
Yao-Tien Chen
The study proposes a novel approach for segmentation and visualization plus value-added surface area and volume measurements for brain medical image analysis. The proposed method contains edge detection and Bayesian based level set segmentation, surface and volume rendering, and surface area and volume measurements for 3D objects of interest (i.e., brain tumor, brain tissue, or whole brain). Two extensions based on edge detection and Bayesian level set are first used to segment 3D objects. Ray casting and a modified marching cubes algorithm are then adopted to facilitate volume and surface visualization of medical-image dataset...
February 17, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28218557/scandcleft-randomised-trials-of-primary-surgery-for-unilateral-cleft-lip-and-palate-7-occlusion-in-5-year-olds-according-to-the-huddart-and-bodenham-index
#5
Agneta Karsten, Agneta Marcusson, Kirsti Hurmerinta, Arja Heliövaara, Annelise Küseler, Pål Skaare, Haydn Bellardie, Elisabeth Rønning, William Shaw, Kirsten Mølsted, Paul Sæle, Eli Brinck, Sara Rizell, Midia Najal Chalier, Philip Eyres, Gunvor Semb
BACKGROUND AND AIM: Good dentofacial development and good occlusion are main goals in the treatment of UCLP. The aim was to evaluate dental occlusion at age 5 years with the Huddart and Bodenham index after four different protocols of primary surgery for UCLP. DESIGN: Three parallel randomised controlled trials were undertaken as an international multicentre study by 10 cleft teams in five countries: Denmark, Finland, Sweden, Norway, and the UK. METHODS: Three different surgical procedures for primary palatal repair (Arms B, C, and D) were tested against a common procedure (Arm A) in the total cohort of 448 children born with non-syndromic UCLP...
February 2017: Journal of Plastic Surgery and Hand Surgery
https://www.readbyqxmd.com/read/28203007/-diagnossis-and-treatment-of-complicated-anterior-teeth-esthetic-defects-by-combination-of-whole-process-digital-esthetic-rehabilitation-with-periodontic-surgery
#6
Z Li, Y S Liu, H Q Ye, Y S Liu, W J Hu, Y S Zhou
OBJECTIVE: To explore a new method of whole-process digital esthetic prosthodontic rehabilitation combined with periodontic surgery for complicated anterior teeth esthetic defects accompanied by soft tissue morphology, to provide an alternative choice for solving this problem under the guidance of three-dimensional (3D) printing digital dental model and surgical guide, thus completing periodontic surgery and digital esthetic rehabilitation of anterior teeth. METHODS: In this study, 12 patients with complicated esthetic problems accompanied by soft tissue morphology in their anterior teeth were included...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28195372/conjugated-polymer-blends-for-organic-photovoltaics-rational-control-of-vertical-stratification-for-high-performance
#7
REVIEW
Yu Yan, Xuan Liu, Tao Wang
The photoactive layer of bulk-heterojunction organic solar cells, in a thickness range of tens to hundreds of nanometers, comprises phase-separated electron donors and acceptors after solution casting. The component distribution in the cross-section of these thin films is found to be heterogeneous, with electron donors or acceptors accumulated or depleted near the electrode interfaces. This vertical stratification of the photovoltaic blend influences device metrics through its impact on charge transport and recombination, and consequently plays an important role in determining the power conversion efficiency of photovoltaic devices...
February 13, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28192927/improving-the-resolution-of-3d-printed-molds-for-microfluidics-by-iterative-casting-shrinkage-cycles
#8
Miao Sun, Yanbo Xie, Jihong Zhu, Jun Li, Jan C T Eijkel
Breaking through technical barriers and cost reduction are critical issues for the development of microfluidic devices, and both rely greatly on the innovation of fabrication techniques and use of new materials. The application of 3D printing definitely accelerated the prototyping of microfluidic chips by its versatility and functionality. However, the resolution of existing 3D printing techniques is still far below that of lithography, which makes it difficult to work on the scale of single cells and near impossible for single molecule work...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28165584/triblock-copolymers-based-on-%C3%AE%C2%B5-caprolactone-and-trimethylene-carbonate-for-the-3d-printing-of-tissue-engineering-scaffolds
#9
Aysun Güney, Jos Malda, Wouter J A Dhert, Dirk W Grijpma
BACKGROUND: Biodegradable PCL-b-PTMC-b-PCL triblock copolymers based on trimethylene carbonate (TMC) and ε-caprolactone (CL) were prepared and used in the 3D printing of tissue engineering scaffolds. Triblock copolymers of various molecular weights containing equal amounts of TMC and CL were prepared. These block copolymers combine the low glass transition temperature of amorphous PTMC (approximately -20°C) and the semi-crystallinity of PCL (glass transition approximately -60°C and melting temperature approximately 60°C)...
February 1, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28165334/sensitivity-of-simulated-transcranial-ultrasound-fields-to-acoustic-medium-property-maps
#10
James L B Robertson, Eleanor Martin, Ben Cox, Bradley Treeby
High intensity transcranial focused ultrasound is an FDA approved treatment for essential tremor, while low-intensity applications such as neurostimulation and opening the blood brain barrier are under active research. Simulations of transcranial ultrasound propagation are used both for focusing through the skull, and predicting intracranial fields. Maps of the skull acoustic properties are necessary for accurate simulations, and can be derived from medical images using a variety of methods. The skull maps range from segmented, homogeneous models, to fully heterogeneous models derived from medical image intensity...
February 6, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28155699/contrast-enhanced-micro-ct-imaging-in-murine-carotid-arteries-a-new-protocol-for-computing-wall-shear-stress
#11
Ruoyu Xing, David De Wilde, Gayle McCann, Yanto Ridwan, Jelle T C Schrauwen, Anton F W van der Steen, Frank J H Gijsen, Kim Van der Heiden
BACKGROUND: Wall shear stress (WSS) is involved in the pathophysiology of atherosclerosis. The correlation between WSS and atherosclerosis can be investigated over time using a WSS-manipulated atherosclerotic mouse model. To determine WSS in vivo, detailed 3D geometry of the vessel network is required. However, a protocol to reconstruct 3D murine vasculature using this animal model is lacking. In this project, we evaluated the adequacy of eXIA 160, a small animal contrast agent, for assessing murine vascular network on micro-CT...
December 28, 2016: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28149954/colloidal-covalent-organic-frameworks
#12
Brian J Smith, Lucas R Parent, Anna C Overholts, Peter A Beaucage, Ryan P Bisbey, Anton D Chavez, Nicky Hwang, Chiwoo Park, Austin M Evans, Nathan C Gianneschi, William R Dichtel
Covalent organic frameworks (COFs) are two- or three-dimensional (2D or 3D) polymer networks with designed topology and chemical functionality, permanent porosity, and high surface areas. These features are potentially useful for a broad range of applications, including catalysis, optoelectronics, and energy storage devices. But current COF syntheses offer poor control over the material's morphology and final form, generally providing insoluble and unprocessable microcrystalline powder aggregates. COF polymerizations are often performed under conditions in which the monomers are only partially soluble in the reaction solvent, and this heterogeneity has hindered understanding of their polymerization or crystallization processes...
January 25, 2017: ACS Central Science
https://www.readbyqxmd.com/read/28149810/trans-oral-glasses-free-three-dimensional-endoscopic-thyroidectomy-preliminary-single-center-experiences
#13
Yi-Ke Zeng, Zhi-Yu Li, Wen-Liang Xuan, Jian-Xing He
Glass-free three-dimensional (3D) endoscopic provide excellent depth perception without decreasing light quality and fog formation. Herein we report our first case-serial of trans-oral endoscopic thyroidectomy by means of our newly developed glasses-free 3D endoscopic system. Four patients with thyroid goiter undergone trans-oral glasses-free 3D endosocpic thyroidectomy were reviewed. Mean BMI of these patients was 20.98±2.91 kg/m(2). The dominate nodule of the thyroid was no more than 5 cm in diameter in all patients...
December 2016: Gland Surgery
https://www.readbyqxmd.com/read/28131890/a-new-generation-of-magnetoencephalography-room-temperature-measurements-using-optically-pumped-magnetometers
#14
Elena Boto, Sofie S Meyer, Vishal Shah, Orang Alem, Svenja Knappe, Peter Kruger, T Mark Fromhold, Mark Lim, Paul M Glover, Peter G Morris, Richard Bowtell, Gareth R Barnes, Matthew J Brookes
Advances in the field of quantum sensing mean that magnetic field sensors, operating at room temperature, are now able to achieve sensitivity similar to that of cryogenically cooled devices (SQUIDs). This means that room temperature magnetoencephalography (MEG), with a greatly increased flexibility of sensor placement can now be considered. Further, these new sensors can be placed directly on the scalp surface giving, theoretically, a large increase in the magnitude of the measured signal. Here, we present recordings made using a single optically-pumped magnetometer (OPM) in combination with a 3D-printed head-cast designed to accurately locate and orient the sensor relative to brain anatomy...
January 25, 2017: NeuroImage
https://www.readbyqxmd.com/read/28131012/uniqueness-of-the-anterior-dentition-three-dimensionally-assessed-for-forensic-bitemark-analysis
#15
A Franco, G Willems, Phc Souza, W Coucke, P Thevissen
The uniqueness of the human dentition (UHD) is an important concept in the comparative process in bitemark analysis. During this analysis, the incisal edges of the suspects' teeth are matched with the bitemarks collected from the victim's body or crime scenes. Despite playing an essential part to exclude suspects, the UHD contained in the involved incisal tooth edges remains an assumption on bitemark level. The present study was aimed, first, to investigate three-dimensionally (3D) the UHD within different quantities of dental material from the incisal edges; second, to test these outcomes in a bidimensional (2D) simulation...
February 2017: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/28129172/mr360-mixed-reality-rendering-for-360-%C3%A2-panoramic-videos
#16
Taehyun Rhee, Lohit Petikam, Benjamin Allen, Andrew Chalmers
This paper presents a novel immersive system called MR360 that provides interactive mixed reality (MR) experiences using a conventional low dynamic range (LDR) 360 (°) panoramic video (360-video) shown in head mounted displays (HMDs). MR360 seamlessly composites 3D virtual objects into a live 360-video using the input panoramic video as the lighting source to illuminate the virtual objects. Image based lighting (IBL) is perceptually optimized to provide fast and believable results using the LDR 360-video as the lighting source...
January 23, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/28110854/training-in-cerebral-aneurysm-clipping-using-self-made-3-dimensional-models
#17
Toshihiro Mashiko, Naoki Kaneko, Takehiko Konno, Keisuke Otani, Rie Nagayama, Eiju Watanabe
INTRODUCTION: Recently, there have been increasingly fewer opportunities for junior surgeons to receive on-the-job training. Therefore, we created custom-built three-dimensional (3D) surgical simulators for training in connection with cerebral aneurysm clipping. METHODS: Three patient-specific models were composed of a trimmed skull, retractable brain, and a hollow elastic aneurysm with its parent artery. The brain models were created using 3D printers via a casting technique...
January 16, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28109797/evaluation-of-sleep-bruxism-with-a-novel-designed-occlusal-splint
#18
Kentaro Hirai, Tomoko Ikawa, Yuko Shigeta, Shuji Shigemoto, Takumi Ogawa
PURPOSE: This report presents our evaluation system that assesses sleep bruxism. The characteristics and fabrication process of our novel designed splint, and the analysis process of our system are presented. METHODS: The subjects were 17 volunteers. The splint was fabricated with a self-curing resin compounded with an amino-acid powder for easy wear on the semi-adjustable articulator, and adjusted for a full-balanced occlusion. An impression of the splint, located on the cast, was taken before and after it was worn...
January 18, 2017: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/28102070/well-ordered-oxygen-deficient-comoo4-and-fe2o3-nanoplate-arrays-on-3d-graphene-foam-toward-flexible-asymmetric-supercapacitors-with-enhanced-capacitive-properties
#19
Kai Chi, Zheye Zhang, Qiying Lv, Chuyi Xie, Jian Xiao, Fei Xiao, Shuai Wang
In this work, we report the development of well-ordered hydrogenated CoMoO4 (H-CoMoO4) and hydrogenated Fe2O3 (H-Fe2O3) nanoplate arrays on 3D graphene foam (GF) and explore their practice application as binder-free electrodes in assembling flexible all-solid-state asymmetric supercapacitor (ASC) devices. Our results show that the monolithic 3D porous GF prepared by solution casting method using Ni foam template possesses large surface area, superior electrical conductivity, and sufficient surface functional groups, which not only facilitate in situ growth of CoMoO4 and Fe2O3 nanoplates but also contribute the double-layer capacitance of the resultant supercapacitor...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28088072/microstructural-mechanical-and-corrosion-characteristics-of-heat-treated-mg-1-2zn-0-5ca-wt-alloy-for-use-as-resorbable-bone-fixation-material
#20
Hamdy Ibrahim, Andrew D Klarner, Behrang Poorganji, David Dean, Alan A Luo, Mohammad Elahinia
Mg-Zn-Ca alloys have grabbed most of the recent attention in research attempting to develop an Mg alloy for bone fixation devices due to their superior biocompatibility. However, early resorption and insufficient strength remain the main problems that hinder their use. Heat treatment has previously been thoroughly studied as a post-shaping process, especially after the fabrication of complex parts (e.g. porous structures) by 3D-printing or powder metallurgy. In this work, the effect of heat treatment on Mg-1...
January 5, 2017: Journal of the Mechanical Behavior of Biomedical Materials
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