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https://www.readbyqxmd.com/read/28330118/cloning-expression-purification-and-characterization-of-lipase-from-bacillus-licheniformis-isolated-from-hot-spring-of-himachal-pradesh-india
#1
Gagandeep Kaur, Amninder Singh, Rohit Sharma, Vinay Sharma, Swati Verma, Pushpender K Sharma
In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusion bodies demonstrated ~22 kDa protein band on 12 % SDS-PAGE. It exhibited specific activity of 0.49 U mg(-1) and % yield of 8.58. Interestingly, the lipase displayed activity at wide range of pH and temperature, i...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330066/endolymphatic-hydrops-imaging-why-oving-from-semi-quantitative-to-anatomical-based-evaluation
#2
Michael Eliezer, Irène Tropres, Alexandre Krainik, Arnaud Attye
INTRODUCTION: Recently, the semi-quantitative ratio grading for endolymphatic hydrops (EH) was found as none specific of MD because all healthy subjects referred with at least one hydrops location with this grading method and 3D-FLAIR sequence. The objectives of this study were to assess the EH prevalence in MD using both the semi-quantitative ratio method of grading and the Saccule to Utricle Ration Inversion (SURI) technique with two 3D-FLAIR sequences, closely similar except for the inversion time of the sequence...
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28329909/testing-the-boundaries-of-boundary-extension-anticipatory-scene-representation-across-development-and-disorder
#3
G Spanò, H Intraub, J O Edgin
Recent studies have suggested that Boundary Extension (BE), a scene construction error, may be linked to the function of the hippocampus. In this study, we tested BE in two groups with variations in hippocampal development and disorder: a typically developing sample ranging from preschool to adolescence and individuals with Down syndrome. We assessed BE across three different test modalities: drawing, visual recognition, and a 3D scene boundary reconstruction task. Despite confirmed fluctuations in memory function measured through a neuropsychological assessment, the results showed consistent BE in all groups across test modalities, confirming the near universal nature of BE...
March 22, 2017: Hippocampus
https://www.readbyqxmd.com/read/28329338/successful-treatment-of-bronchial-stenosis-after-lung-transplantation
#4
Irene T Schrijver, Bart Luijk, Ronald C A Meijer, Linda M de Heer
Treatment of stenotic anastomosis after lung transplantation can be challenging. In this case report, we present a case in which 3D computed tomography reconstructions guided the clinical decision towards operative bronchoplasty after which our patient was treated successfully.
February 23, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28329230/3d-echocardiographic-reference-ranges-for-normal-left-ventricular-volumes-and-strain-results-from-the-eacvi-norre-study
#5
Anne Bernard, Karima Addetia, Raluca Dulgheru, Luis Caballero, Tadafumi Sugimoto, Natela Akhaladze, George D Athanassopoulos, Daniele Barone, Monica Baroni, Nuno Cardim, Andreas Hagendorff, Krasimira Hristova, Federica Ilardi, Teresa Lopez, Gonzalo de la Morena, Bogdan A Popescu, Martin Penicka, Tolga Ozyigit, Jose David Rodrigo Carbonero, Nico van de Veire, Ralph Stephan Von Bardeleben, Dragos Vinereanu, Jose Luis Zamorano, Christophe Martinez, Julien Magne, Bernard Cosyns, Erwan Donal, Gilbert Habib, Luigi P Badano, Roberto M Lang, Patrizio Lancellotti
Aim: To obtain the normal ranges for 3D echocardiography (3DE) measurement of left ventricular (LV) volumes, function, and strain from a large group of healthy volunteers. Methods and results: A total of 440 (mean age: 45 ± 13 years) out of the 734 healthy subjects enrolled at 22 collaborating institutions of the Normal Reference Ranges for Echocardiography (NORRE) study had good-quality 3DE data sets that have been analysed with a vendor-independent software package allowing homogeneous measurements regardless of the echocardiographic machine used to acquire the data sets...
December 22, 2016: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329229/isolated-severe-leftward-displacement-of-the-septum-primum-anatomic-and-3d-echocardiographic-findings-and-surgical-repair
#6
Fabio Cuttone, Khaled Hadeed, François Lacour-Gayet, Hugues Lucron, Sebastien Hascoet, Philippe Acar, Bertrand Leobon, Richard Van Praagh
OBJECTIVES: Leftward displacement of the septum primum is usually described as associated with hypoplastic left heart syndrome or visceral heterotaxy. This rare malformation results in partially or totally anomalous pulmonary venous drainage with a normal connection of the pulmonary veins to the left atrium, depending on the degree of septal shift. We report the 3D echocardiographic and anatomic findings as well as the surgical repair in a series of isolated severe leftward displacement of the septum primum, responsible for totally anomalous pulmonary venous drainage...
February 16, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28329129/first-experience-with-real-time-3d-anatomical-fusion-imaging-during-left-atrial-appendage-occluder-implantation
#7
Shazia Afzal, Dagmar Soetemann, Niels Nijhof, Malte Kelm, Tobias Zeus
No abstract text is available yet for this article.
February 28, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329105/medical-three-dimensional-printing-opens-up-new-opportunities-in-cardiology-and-cardiac-surgery
#8
Thomas Bartel, Andrew Rivard, Alejandro Jimenez, Carlos A Mestres, Silvana Müller
Advanced percutaneous and surgical procedures in structural and congenital heart disease require precise pre-procedural planning and continuous quality control. Although current imaging modalities and post-processing software assists with peri-procedural guidance, their capabilities for spatial conceptualization remain limited in two- and three-dimensional representations. In contrast, 3D printing offers not only improved visualization for procedural planning, but provides substantial information on the accuracy of surgical reconstruction and device implantations...
February 16, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28329082/aortic-elongation-and-the-risk-for-dissection-the-t%C3%A3-bingen-aortic-pathoanatomy-taipan-project%C3%A2
#9
Tobias Krüger, Alexandre Oikonomou, David Schibilsky, Mario Lescan, Katharina Bregel, Luise Vöhringer, Wilke Schneider, Henning Lausberg, Gunnar Blumenstock, Fabian Bamberg, Christian Schlensak
OBJECTIVES: We measured aortic dimensions, particularly length parameters, using 3D imaging with the aim of refining the risk-morphology for Stanford type A aortic dissection (TAD). METHODS: Computer tomography angiography studies were analysed using the curved multiplanar reformats. At defined landmarks, the diameters and lengths of aortic segments were recorded. Three groups were compared retrospectively: patients actually suffering from a TAD (TAD-group; n  = 150), patients before suffering a TAD (preTAD-group n  = 15) and a healthy control group ( n  = 215)...
February 16, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28329054/whole-heart-detailed-and-quantitative-anatomy-myofibre-structure-and-vasculature-from-x-ray-phase-contrast-synchrotron-radiation-based-micro-computed-tomography
#10
Anna Gonzalez-Tendero, Chong Zhang, Vedrana Balicevic, Rubén Cárdenes, Sven Loncaric, Constantine Butakoff, Bruno Paun, Anne Bonnin, Patricia Garcia-Cañadilla, Emma Muñoz-Moreno, Eduard Gratacós, Fatima Crispi, Bart Bijnens
Background: While individual cardiac myocytes only have a limited ability to shorten, the heart efficiently pumps a large volume-fraction thanks to a cell organization in a complex 3D fibre structure. Subclinical subtle cardiac structural remodelling is often present before symptoms arise. Understanding and early detection of these subtle changes is crucial for diagnosis and prevention. Additionally, personalized computational modelling requires knowledge on the multi-scale structure of the whole heart and vessels...
March 15, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28328995/effectiveness-of-interceptive-treatment-of-class-iii-malocclusions-with-skeletal-anchorage-a-systematic-review-and-meta-analysis
#11
Jorge Rodríguez de Guzmán-Barrera, Carla Sáez Martínez, Montserrat Boronat-Catalá, Jose María Montiel-Company, Vanessa Paredes-Gallardo, José Luís Gandía-Franco, José Manuel Almerich-Silla, Carlos Bellot-Arcís
Recently, new strategies for treating class III malocclusions have appeared. Skeletal anchorage appears to reduce the dentoalveolar effects while maximising the orthopaedic effect in growing patients. The purpose of this systematic review and meta-analysis is to examine the effectiveness of bone anchorage devices for interceptive treatment of skeletal class III malocclusions. Searches were made in the Pubmed, Embase, Scopus and Cochrane databases, as well as in a grey literature database, and were complemented by hand-searching...
2017: PloS One
https://www.readbyqxmd.com/read/28328931/x-ray-microtomography-for-ant-taxonomy-an-exploration-and-case-study-with-two-new-terataner-hymenoptera-formicidae-myrmicinae-species-from-madagascar
#12
Francisco Hita Garcia, Georg Fischer, Cong Liu, Tracy L Audisio, Gary D Alpert, Brian L Fisher, Evan P Economo
We explore the potential of x-ray micro computed tomography (μCT) for the field of ant taxonomy by using it to enhance the descriptions of two remarkable new species of the ant genus Terataner: T. balrog sp. n. and T. nymeria sp. n.. We provide an illustrated worker-based species identification key for all species found on Madagascar, as well as detailed taxonomic descriptions, which include diagnoses, discussions, measurements, natural history data, high-quality montage images and distribution maps for both new species...
2017: PloS One
https://www.readbyqxmd.com/read/28328842/three-dimensional-printing-and-computer-navigation-assisted-hemipelvectomy-for-en-bloc-resection-of-osteochondroma-a-case-report
#13
Yaqing Zhang, Lianjiang Wen, Jun Zhang, Guoliang Yan, Yue Zhou, Bo Huang
RATIONALE: Three-dimensional (3D) printed templates can be designed to match an individual's anatomy, allowing surgeons to refine preoperative planning. In addition, the use of computer navigation (NAV) is gaining popularity to improve surgical accuracy in the resection of pelvic tumors. However, its use in combination with 3D printing to assist complex pelvic tumor resection has not been reported. PATIENT CONCERNS: A 36-year-old man presented with left-sided pelvic pain and a fast-growing mass...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28328637/estimating-implant-volume-and-mastectomy-specimen-volume-by-measuring-breast-volume-with-a-3-dimensional-scanner
#14
Hiroki Utsunomiya, Taro Kusano, Nobuhiro Sato, Shinya Yoshimoto
OBJECTIVES: Conventional 2-stage expander-implant breast reconstruction is frequently performed. However, direct to implant reconstruction should be considered if indicated and if circumstances allow. One difficulty in breast reconstruction postmastectomy is selection of the appropriate implant size prior to surgery. The ability to estimate implant volume and mastectomy-specimen volume from the preoperative breast volume using a 3-dimensional (3D) scanner would greatly facilitate reconstruction...
March 21, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28328541/molecular-engineering-of-two-photon-fluorescent-probes-for-bioimaging-applications
#15
Hong-Wen Liu, Yongchao Liu, Peng Wang, Xiao-Bing Zhang
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328289/prospective-3d-analysis-of-facial-soft-tissue-augmentation-with-calcium-hydroxylapatite
#16
Filip Simunovic, Stefan Schlager, Michaela Montanari, Niklas Iblher
BACKGROUND: Facial rejuvenation is an expanding field with an increasing number of treatment modalities. Several non-autologous filler materials are available for soft tissue augmentation. Calcium hydroxylapatite (CaOH) is aimed at increasing collagen neosynthesis and thereby producing long term augmentation effects. Despite a multitude of observational reports, the field is suffering from lack of quantitative morphometic evaluation methods. OBJECTIVE: The objective of this proof-of-principle study was to investigate whether the effects of facial tissue augmentation with CaOH (Radiesse™) can be quantified and followed up using 3D surface scanning...
March 22, 2017: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/28328215/synthesis-and-mechanical-character-of-hexagonal-phase-%C3%AE-wn
#17
Changchun Wang, Qiang Tao, Shushan Dong, Xin Wang, Pinwen Zhu
In this work, high-quality bulk WC-structured WN (δ-WN) was synthesized via an untraditional method and the structure was accurately determined by X-ray diffraction and Rietveld refinement. In the process of synthesizing δ-WN, W2N3 and melamine were used as tungsten source and nitrogen source, respectively. The result of successfully synthesized high-quality δ-WN indicates that our method is an effective route for synthesizing high-quality bulk δ-WN and melamine is a pure nitrogen source for introducing the nitrogen to the metal precursor...
March 22, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28328200/fluorescence-resonance-energy-transfer-based-dna-tetrahedron-nano-tweezer-for-highly-reliable-detection-of-tumor-related-mrna-in-living-cells
#18
Lei He, Dan-Qing Lu, Hao Liang, Sitao Xie, Can Luo, Miaomiao Hu, Liujun Xu, Xiaobing Zhang, Weihong Tan
Accurate detection and imaging of tumor-related mRNA in living cells hold great promise for early cancer detection. However, currently, most probes designed to image intracellular mRNA confront intrinsic interferences arising from complex biological matrices and resulting in inevitable false-positive signals. To circumvent this problem, a new class of intracellular DNA nanoprobe, termed DNA tetrahedron nano-tweezer (DTNT), was developed to reliably image tumor-related mRNA in living cells based on the FRET (fluorescence resonance energy transfer) "off" to "on" signal readout mode...
March 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28328100/smart-mechanosensing-machineries-enable-migration-of-vascular-smooth-muscle-cells-in-atherosclerosis-relevant-3d-matrices
#19
REVIEW
Qingjia Chi, Jieling Shan, Xiaorong Ding, Tieying Yin, Yazhou Wang, Dongyu Jia, Guixue Wang
At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechanically-strong basement membrane. Migration of VSMCs is potentially caused by biomechanical mechanism. Most in vitro studies focus on cell migration on 2D substrates in response to biochemical factors...
March 22, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28328084/control-of-nanoparticle-release-kinetics-from-3d-printed-hydrogel-scaffolds
#20
Bernhard Baumann, Tomasz Jungst, Simone Stichler, Susanne Feineis, Oliver Wiltschka, Matthias Kuhlmann, Mika Lindén, Jürgen Groll
The convergence of biofabrication with nanotechnology is largely unexplored but enables geometrical control of cell-biomaterial arrangement combined with controlled drug delivery and release. As a step towards integration of these two fields of research, this study demonstrates that modulation of electrostatic nanoparticle-polymer and nanoparticle-nanoparticle interactions can be used for tuning nanoparticle release kinetics from 3D printed hydrogel scaffolds. This generic strategy can be used for spatiotemporal control of the release kinetics of nanoparticulate drug vectors in biofabricated constructs...
March 22, 2017: Angewandte Chemie
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