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https://www.readbyqxmd.com/read/28919716/palatability-and-physical-properties-of-potassium-binding-resin-rdx7675-comparison-with-sodium-polystyrene-sulfonate
#1
Vanessa Zann, John McDermott, Jeffrey W Jacobs, James P Davidson, Fangling Lin, Paul Korner, Robert C Blanks, David P Rosenbaum
BACKGROUND: Hyperkalemia is a potentially life-threatening condition that patients with heart failure or chronic kidney disease, especially those taking renin-angiotensin-aldosterone system inhibitors, are at high risk of developing. Sodium polystyrene sulfonate (SPS), a current treatment, binds potassium within the gastrointestinal tract to reduce potassium absorption. However, poor palatability limits its long-term use. RDX7675, a novel potassium binder in development for the treatment of hyperkalemia, is a calcium salt of a reengineered polystyrene sulfonate-based resin designed to have enhanced palatability...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28917725/a-computational-approach-for-phenotypic-comparisons-of-cell-populations-in-high-dimensional-cytometry-data
#2
Ludovic Platon, David Pejoski, Guillaume Gautreau, Brice Targat, Roger Le Grand, Anne-Sophie Beignon, Nicolas Tchitchek
BACKGROUND: Cytometry is an experimental technique used to measure molecules expressed by cells at a single cell resolution. Recently, several technological improvements have made possible to increase greatly the number of cell markers that can be simultaneously measured. Many computational methods have been proposed to identify clusters of cells having similar phenotypes. Nevertheless, only a limited number of computational methods exist to compare the phenotypes of the cell clusters identified by different clustering approaches...
September 13, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28916460/the-effect-of-viroid-infection-of-citrus-trees-on-the-amoebicidal-activity-of-maltese-half-blood-citrus-sinensis-against-trophozoite-stage-of-acanthamoeba-castellanii-neff
#3
Ghaya Zouaghi, Asma Najar, Olfa Chiboub, Ines Sifaoui, Manef Abderrabba, Jacob Lorenzo Morales
In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three samples of peels' essential oils were studied: from a healthy plant (Control), a plant inoculated with Citrus exocortis viroid (CEVd) and one inoculated with hot stunt cachexia viroid (HSVd). The samples were extracted by hydrodistillation from dried peels and characterized by GC-MS...
September 12, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28912786/co-infection-of-sweet-orange-with-severe-and-mild-strains-of-citrus-tristeza-virus-is-overwhelmingly-dominated-by-the-severe-strain-on-both-the-transcriptional-and-biological-levels
#4
Shimin Fu, Jonathan Shao, Changyong Zhou, John S Hartung
Citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted Closterovirus, Citrus tristeza virus. Mild strain CTV-B2 does not cause obvious symptoms on indicators whereas severe strain CTV-B6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. Our laboratory has previously characterized changes in transcription in sweet orange separately infected with CTV-B2 and CTV-B6. In the present study, transcriptome analysis of Citrus sinensis in response to double infection by CTV-B2 and CTV-B6 was carried out...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28911641/evaluation-of-the-prebiotic-effects-of-citrus-pectin-hydrolysate
#5
Yen-Yi Ho, Chia-Min Lin, Ming-Chang Wu
Citrus pectin enzyme hydrolysate (PEH) of different hydrolysis time intervals (6 hours, PEH-6; 12 hours, PEH-12; 24 hours, PEH-24; or 48 hours, PEH-48) or concentrations (1%, 2%, and 4%) was tested for its growth stimulation effect on two probiotics, Bifidobacterium bifidum and Lactobacillus acidophilus. Higher monosaccharide concentrations and smaller molecular weights of PEHs were obtained by prolonging the hydrolysis time. In addition, higher PEH concentrations resulted in significantly higher (p < 0...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911634/extraction-and-quantification-of-polyphenols-from-kinnow-citrus-reticulate-l-peel-using-ultrasound-and-maceration-techniques
#6
Muhammad N Safdar, Tusneem Kausar, Saqib Jabbar, Amer Mumtaz, Karam Ahad, Ambreen A Saddozai
An investigation was carried out to extract polyphenols from the peel of kinnow (Citrus reticulate L.) by maceration and ultrasound-assisted extraction (UAE) techniques. The antioxidant potential of these polyphenols was evaluated using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide radical scavenging assays; and their antimicrobial activity was assessed against bacterial strains Staphyloccoccus aureus, Bacillus cereus, and Salmonella typhimurium. The highest extraction yield was obtained through the solvent ethanol at 80% concentration level, whereas UAE was a more efficient technique and yielded comparatively higher polyphenol contents than maceration...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911545/chemistry-and-health-effects-of-furanocoumarins-in-grapefruit
#7
REVIEW
Wei-Lun Hung, Joon Hyuk Suh, Yu Wang
Furanocoumarins are a specific group of secondary metabolites that commonly present in higher plants, such as citrus plants. The major furanocoumarins found in grapefruits (Citrus paradisi) include bergamottin, epoxybergamottin, and 6',7'-dihydroxybergamottin. During biosynthesis of these furanocoumarins, coumarins undergo biochemical modifications corresponding to a prenylation reaction catalyzed by the cytochrome P450 enzymes with the subsequent formation of furan rings. Because of undesirable interactions with several medications, many studies have developed methods for grapefruit furanocoumarin quantification that include high-performance liquid chromatography coupled with UV detector or mass spectrometry...
January 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911534/phenolic-compounds-and-biological-activities-of-small-size-citrus-kumquat-and-calamondin
#8
REVIEW
Shyi-Neng Lou, Chi-Tang Ho
Kumquat and calamondin are two small-size citrus fruits. Owing to their health benefits, they are traditionally used as folk medicine in Asian countries. However, the research on flavonoids and biological activities of kumquat and calamondin have received less attention. This review summarizes the reported quantitative and qualitative data of phenolic compositions in these two fruits. Effects of maturity, harvest time, various solvent extractions and heat treatment of phenolic compositions, and bioactivities were discussed; distributions of the forms of phenolic compounds existing in kumquat and calamondin were also summarized...
January 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910703/long-term-manganese-toxicity-induced-alterations-of-physiology-and-leaf-protein-profiles-in-two-citrus-species-differing-in-manganese-tolerance
#9
Xiang You, Lin-Tong Yang, Yi-Ping Qi, Peng Guo, Ning-Wei Lai, Xin Ye, Qiang Li, Li-Song Chen
Manganese (Mn)-intolerant 'Sour pummelo' (Citrus grandis) and Mn-tolerant 'Xuegan' (Citrus sinensis) seedlings were irrigated for 17 weeks with 2 (control) or 600μM (Mn-toxicity or -excess) MnSO4. C. sinensis had higher Mn-tolerance than C. grandis, as indicated by the higher photosynthesis rates in Mn-excess C. sinensis leaves. Under Mn-toxicity, Mn levels were similar between C. sinensis and C. grandis roots, but lower in C. sinensis leaves than in C. grandis leaves. This might be responsible for C. sinensis Mn-tolerance...
September 2, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28910375/application-of-droplet-digital-pcr-for-quantitative-detection-of-spiroplasma-citri-in-comparison-with-real-time-pcr
#10
Yogita Maheshwari, Vijayanandraj Selvaraj, Subhas Hajeri, Raymond Yokomi
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of external standards. This study evaluated the applicability of ddPCR as a quantitative detection tool for the Spiroplasma citri, causal agent of citrus stubborn disease (CSD) in citrus. Two sets of primers, SP1, based on the spiral in housekeeping gene, and a multicopy prophage gene, SpV1 ORF1, were used to evaluate ddPCR in comparison with real time (quantitative) PCR (qPCR) for S...
2017: PloS One
https://www.readbyqxmd.com/read/28906110/anti-in%C3%AF-ammatory-effect-of-essential-oil-from-citrus-aurantium-l-var-amara-engl
#11
Chun-Yan Shen, Jian-Guo Jiang, Qin Ou-Yang, Wei Zhu
Essential oil has been popularly used as an alternative for the treatment of inflammation. The bioactivities of essential oil from blossoms of Citrus aurantium L. var. amara Engl (CAVAO) showed greater anti-inflammation potential than that of antioxidant, anticancer, and 3T3-L1 proliferation inhibition. CAVAO (250 μg/mL) significantly inhibited production of nitric oxide (NO) (99.54%±2.81), interleukin-6 (IL-6) (98.11%±1.62), tumor necrosis factor-alpha (TNF-α) (41.84%±1.52) and interleukin-1beta (IL-1β) (56...
September 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28904944/antibacterial-and-antibiotic-modifying-activity-of-methanol-extracts-from-six-cameroonian-food-plants-against-multidrug-resistant-enteric-bacteria
#12
Joachim K Dzotam, Victor Kuete
The present work was designed to investigate the antibacterial activities of methanol extracts from six Cameroonian edible plants and their synergistic effects with some commonly used antibiotics against multidrug-resistant (MDR) Gram-negative bacteria expressing active efflux pumps. The extracts were subjected to qualitative phytochemical screening and the microdilution broth method was used for antibacterial assays. The results of phytochemical tests indicate that all tested crude extracts contained polyphenols, flavonoids, triterpenes, and steroids...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28904803/comparative-transcriptome-analysis-during-early-fruit-development-between-three-seedy-citrus-genotypes-and-their-seedless-mutants
#13
Shujian Zhang, Qingchun Shi, Ute Albrecht, Robert G Shatters, Ric Stange, Greg McCollum, Shuo Zhang, Chengming Fan, Ed Stover
Identification of genes with differential transcript abundance (GDTA) in seedless mutants may enhance understanding of seedless citrus development. Transcriptome analysis was conducted at three time points during early fruit development (Phase 1) of three seedy citrus genotypes: Fallglo (Bower citrus hybrid (Citrus reticulata×C. reticulata×C. paradisi)×Temple (C. reticulata×C. sinensis)), grapefruit (C. paradisi), Pineapple sweet orange (C. sinensis), and their seedless mutants. Seed abortion in seedless mutants was observed at 26 days post anthesis (Time point 2)...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28904369/antimutagenic-and-antioxidant-activity-of-the-essential-oils-of-citrus-sinensis-and-citrus-latifolia
#14
J D Toscano-Garibay, M Arriaga-Alba, J Sánchez-Navarrete, M Mendoza-García, J J Flores-Estrada, M A Moreno-Eutimio, J J Espinosa-Aguirre, M González-Ávila, N J Ruiz-Pérez
The essential oils of Citrus sinensis and Citrus latifolia showed antimycotic activity against Candida spp. isolated from the oral cavity; they are neither mutagenic on the Ames test nor cytotoxic. Their main components are R-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. The aim of this work was to evaluate their antimutagenic and antioxidant capacities. Antimutagenic properties were evaluated against MNNG and ENNG on S. typhimurium TA100; against 2AA on strain TA98 and in front of 4NQO and NOR on strain TA102...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28904280/food-dependent-exercise-induced-anaphylaxis-due-to-ingestion-of-mikan-citrus-unshiu
#15
Yutaka Tanaka, Marie Sugihara, Shuntaro Tsumagari, Nobue Takamatsu, Kazuyuki Kurihara
A 12-year-old girl was referred to our hospital owing to repeated anaphylactic reactions induced by exercise after meals. Food-dependent exercise-induced anaphylaxis (FDEIAn) was suspected. However, sequential tests of typical foods, including egg, milk, soy, and wheat, in combination with exercise, were all negative.The results of the skin prick test (SPT) for Citrus unshiu and specific IgE test for orange and grapefruit were positive. Although no symptoms were noted after an exercise challenge combined with the ingestion of only Citrus unshiu, an anaphylactic reaction was induced by additional acetyl-salicylic acid...
2017: Arerugī, [Allergy]
https://www.readbyqxmd.com/read/28903082/hesperidin-a-citrus-bioflavonoid-alleviates-trichloroethylene-induced-oxidative-stress-in-drosophila-melanogaster
#16
Amos Olalekan Abolaji, Oluwatoyin Victoria Babalola, Abimbola Kehinde Adegoke, Ebenezer Olatunde Farombi
Trichloroethylene (TCE) is a chlorinated organic pollutant of groundwater with diverse toxic effects in animals and humans. Here, we investigated the ameliorative role of hesperidin, a citrus bioflavonoid on TCE-induced toxicity in Drosophila melanogaster. Four groups of D. melanogaster (50 flies/vial, with 5 vials/group) were exposed to ethanol (2.5%, control), HSP (400mg/10g diet), TCE (10μM/10g diet) and TCE (10μM/10g diet)+HSP (400mg/10g diet) respectively in the diet for 5days. Then, selected oxidative stress and antioxidant markers were evaluated...
September 1, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28902955/regulation-of-citrus-responses-to-the-combined-action-of-drought-and-high-temperatures-depends-on-the-severity-of-water-deprivation
#17
Sara I Zandalinas, Damián Balfagón, Vicent Arbona, Aurelio Gómez-Cadenas
Plants grown in natural environment are regularly subjected to different combinations of abiotic stresses. Recent studies revealed that citrus plants subjected to a combination of severe drought and high temperatures displayed specific physiological, hormonal, molecular and metabolic responses. In the present study, we have performed a long-term experiment combining moderate drought and heat in Cleopatra mandarin to evaluate the impact of the stress-sequence, intensity and duration. Our results support previous observation of high sensitivity of Cleopatra mandarin to abiotic stresses that include high temperatures...
September 13, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28900464/synergistic-antioxidant-and-anti-inflammatory-effects-between-modified-citrus-pectin-and-honokiol
#18
Cheppail Ramachandran, Barry Wilk, Steven J Melnick, Isaac Eliaz
Inflammation is a normal physiological process; however, dysregulation of this process may contribute to inflammatory-based chronic disorders and diseases in animals and humans. Therefore, the antioxidant and anti-inflammatory properties of natural products, often recognized in traditional medicine systems, represent therapeutic modalities to reduce or prevent uncontrolled inflammatory processes which in turn potentially ameliorate or prevent sequelae of inflammatory-based symptoms of chronic diseases. We have investigated the antioxidant and anti-inflammatory effects of honokiol (HNK) and modified citrus pectin (MCP) in vitro and examined whether the MCP : HNK combination has synergistic effects on antioxidant and anti-inflammatory properties...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28900440/chlorophyll-fluorescence-imaging-uncovers-photosynthetic-fingerprint-of-citrus-huanglongbing
#19
Haiyan Cen, Haiyong Weng, Jieni Yao, Mubin He, Jingwen Lv, Shijia Hua, Hongye Li, Yong He
Huanglongbing (HLB) is one of the most destructive diseases of citrus, which has posed a serious threat to the global citrus production. This research was aimed to explore the use of chlorophyll fluorescence imaging combined with feature selection to characterize and detect the HLB disease. Chlorophyll fluorescence images of citrus leaf samples were measured by an in-house chlorophyll fluorescence imaging system. The commonly used chlorophyll fluorescence parameters provided the first screening of HLB disease...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28899694/anti-atherosclerotic-effect-of-hesperidin-in-ldlr-mice-and-its-possible-mechanism
#20
Ye-Zi Sun, Jian-Fei Chen, Li-Min Shen, Ji Zhou, Cui-Fang Wang
Hesperidin, a citrus bioflavonoid, exerts numerous pharmacological activities. However, its protective effect against atherosclerosis in vivo remains poorly understood. In the present study, we aimed to observe the effects of hesperidin on high fat diet (HFD)-induced atherosclerosis using LDL receptor deficient (LDLr(-/-)) mice. After 12 weeks of treatment, the animals were sacrificed. The blood samples were collected for further analysis. Mouse peritoneal macrophages were collected. Hepatic lipid content, quantification of atherosclerosis, assessment of oxidative stress and inflammation, gene expressions were performed on liver and aorta samples...
September 9, 2017: European Journal of Pharmacology
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