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https://www.readbyqxmd.com/read/28918486/elastic-and-inelastic-light-scattering-spectroscopy-and-its-possible-use-for-label-free-brain-tumor-typing
#1
Edwin Ostertag, Mona Stefanakis, Karsten Rebner, Rudolf W Kessler
This paper presents an approach for label-free brain tumor tissue typing. For this application, our dual modality microspectroscopy system combines inelastic Raman scattering spectroscopy and Mie elastic light scattering spectroscopy. The system enables marker-free biomedical diagnostics and records both the chemical and morphologic changes of tissues on a cellular and subcellular level. The system setup is described and the suitability for measuring morphologic features is investigated. Graphical Abstract Bimodal approach for label-free brain tumor typing...
September 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28918110/emergent-behaviors-in-rbcs-flows-in-micro-channels-using-digital-particle-image-velocimetry
#2
F Cairone, D Ortiz, P J Cabrales, M Intaglietta, M Bucolo
The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, however their introduction, also, for pathological conditions diagnosis would be important in different biomedical contexts. To overcome this lack is necessary to establish the relation between the RBCs flow and blood viscosity changes in micro-vessels...
September 13, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28916855/green-approach-for-synthesis-of-zinc-oxide-nanoparticles-from-andrographis-paniculata-leaf-extract-and-evaluation-of-their-antioxidant-anti-diabetic-and-anti-inflammatory-activities
#3
Govindasamy Rajakumar, Muthu Thiruvengadam, Govindarasu Mydhili, Thandapani Gomathi, Ill-Min Chung
Bio-mediated synthesis of zinc oxide nanoparticles (ZnO NPs) was carried out by utilizing the reducing and capping potential of Andrographis paniculata leaf extract. The capped ZnO NPs were characterized using UV-Vis, XRD, FTIR, SEM, TEM and SAED analyses. FTIR analysis suggested the role of phenolic compounds, terpenoids, and proteins of A. paniculata leaf extract, in nucleation and stability of ZnO NPs. XRD pattern compared with the standard confirmed spectrum of zinc oxide particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values...
September 15, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28916762/time-resolved-analysis-of-dna-protein-interactions-in-living-cells-by-uv-laser-pulses
#4
Angela Nebbioso, Rosaria Benedetti, Mariarosaria Conte, Vincenzo Carafa, Floriana De Bellis, Jani Shaik, Filomena Matarese, Bartolomeo Della Ventura, Felice Gesuele, Raffaele Velotta, Joost H A Martens, Hendrik G Stunnenberg, Carlo Altucci, Lucia Altucci
Interactions between DNA and proteins are mainly studied through chemical procedures involving bi-functional reagents, mostly formaldehyde. Chromatin immunoprecipitation is used to identify the binding between transcription factors (TFs) and chromatin, and to evaluate the occurrence and impact of histone/DNA modifications. The current bottleneck in probing DNA-protein interactions using these approaches is caused by the fact that chemical crosslinkers do not discriminate direct and indirect bindings or short-lived chromatin occupancy...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916753/automated-n-glycosylation-sequencing-of-biopharmaceuticals-by-capillary-electrophoresis
#5
Marton Szigeti, Andras Guttman
Comprehensive analysis of the N-linked carbohydrates of glycoproteins is gaining high recent interest in both the biopharmaceutical and biomedical fields. In addition to high resolution glycosylation profiling, sugar residue and linkage specific enzymes are also routinely used for exoglycosidase digestion based carbohydrate sequencing. This latter one, albeit introduced decades ago, still mostly practiced by following tedious and time consuming manual processes. In this paper we introduce an automated carbohydrate sequencing approach using the appropriate exoglycosidase enzymes in conjunction with the utilization of some of the features of a capillary electrophoresis (CE) instrument to speed up the process...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28915318/applications-of-high-resolution-magic-angle-spinning-mrs-in-biomedical-studies-ii-human-diseases
#6
REVIEW
Christopher Dietz, Felix Ehret, Francesco Palmas, Lindsey A Vandergrift, Yanni Jiang, Vanessa Schmitt, Vera Dufner, Piet Habbel, Johannes Nowak, Leo L Cheng
High-resolution magic angle spinning (HRMAS) MRS is a powerful method for gaining insight into the physiological and pathological processes of cellular metabolism. Given its ability to obtain high-resolution spectra of non-liquid biological samples, while preserving tissue architecture for subsequent histopathological analysis, the technique has become invaluable for biochemical and biomedical studies. Using HRMAS MRS, alterations in measured metabolites, metabolic ratios, and metabolomic profiles present the possibility to improve identification and prognostication of various diseases and decipher the metabolomic impact of drug therapies...
September 15, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28915238/a-high-resolution-atlas-of-gene-expression-in-the-domestic-sheep-ovis-aries
#7
Emily L Clark, Stephen J Bush, Mary E B McCulloch, Iseabail L Farquhar, Rachel Young, Lucas Lefevre, Clare Pridans, Hiu Tsang, Chunlei Wu, Cyrus Afrasiabi, Mick Watson, C Bruce Whitelaw, Tom C Freeman, Kim M Summers, Alan L Archibald, David A Hume
Sheep are a key source of meat, milk and fibre for the global livestock sector, and an important biomedical model. Global analysis of gene expression across multiple tissues has aided genome annotation and supported functional annotation of mammalian genes. We present a large-scale RNA-Seq dataset representing all the major organ systems from adult sheep and from several juvenile, neonatal and prenatal developmental time points. The Ovis aries reference genome (Oar v3.1) includes 27,504 genes (20,921 protein coding), of which 25,350 (19,921 protein coding) had detectable expression in at least one tissue in the sheep gene expression atlas dataset...
September 15, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28915005/a-novel-type-of-aqueous-dispersible-ultrathin-layered-double-hydroxide-nanosheets-for-in-vivo-bioimaging-and-drug-delivery
#8
Li Yan, Mengjiao Zhou, Xiujuan Zhang, Longbiao Huang, Wei Chen, Vellaisamy A L Roy, WenJun Zhang, Xianfeng Chen
Layered double hydroxide (LDH) nanoparticles have been widely used for various biomedical applications. However, because of the difficulty of surface functionalization of LDH nanoparticles, the systemic administration of these nanomaterials for in vivo therapy remains a bottleneck. In this work, we develop a novel type of aqueous dispersible two dimensional ultrathin LDH nanosheets with a size of about 50 nm and a thickness of about 1.4 to 4 nm. We are able to covalently attach positively charged Rhodamine B fluorescent molecules to the nanosheets and the nanohybrid retains strong fluorescence in liquid and even dry powder form...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28914397/influence-of-acid-treatment-on-surface-properties-and-in-vivo-performance-of-ti6al4v-alloy-for-biomedical-applications
#9
Daniel J Fernandes, Ruy G Marques, Carlos N Elias
The purpose of this work was to investigate the influence of acid treatment on the surface properties and in vivo performance of titanium grade 5 (Ti6Al4V) alloy. Mini-implants with surface treatment were inserted into New Zealand rabbit tibia for 1, 4 and 8 weeks. SEM analysis showed intercommunicated micropores in acid treated samples. AFM showed micron and sub-micron roughness. The thickness of the titanium oxide layer increased with surface treatment, with a significant reduction of Al and V concentration...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28912836/methods-for-enhancing-the-reproducibility-of-biomedical-research-findings-using-electronic-health-records
#10
REVIEW
Spiros Denaxas, Kenan Direk, Arturo Gonzalez-Izquierdo, Maria Pikoula, Aylin Cakiroglu, Jason Moore, Harry Hemingway, Liam Smeeth
BACKGROUND: The ability of external investigators to reproduce published scientific findings is critical for the evaluation and validation of biomedical research by the wider community. However, a substantial proportion of health research using electronic health records (EHR), data collected and generated during clinical care, is potentially not reproducible mainly due to the fact that the implementation details of most data preprocessing, cleaning, phenotyping and analysis approaches are not systematically made available or shared...
2017: BioData Mining
https://www.readbyqxmd.com/read/28912713/meta-analysis-for-clinical-evaluation-of-xingnaojing-injection-for-the-treatment-of-cerebral-infarction
#11
REVIEW
Xiao Ma, Yu X Yang, Nian Chen, Qian Xie, Tao Wang, Xuan He, Jian Wang
Objective: Xingnaojing injection (XNJ) is derived from An-Gong-Niu-Huang pill, a well-known traditional Chinese patent medicine, which is widely used for stroke. To evaluate the therapeutic effect of XNJ on cerebral infarction, an extensive meta-analysis was used. Methods: Six major electronic databases including the Chinese Biomedical Database (CBM), Wanfang, the VIP medicine information system (VMIS) and the China National Knowledge Infrastructure (CNKI), PubMed, Embase, and the Cochrane Library were examined to retrieve randomized controlled trials designed to evaluate the clinical efficacy of XNJ in treating CI before November 26, 2016...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28912565/real-time-image-processing-for-microscopy-based-label-free-imaging-flow-cytometry-in-a-microfluidic-chip
#12
Young Jin Heo, Donghyeon Lee, Junsu Kang, Keondo Lee, Wan Kyun Chung
Imaging flow cytometry (IFC) is an emerging technology that acquires single-cell images at high-throughput for analysis of a cell population. Rich information that comes from high sensitivity and spatial resolution of a single-cell microscopic image is beneficial for single-cell analysis in various biological applications. In this paper, we present a fast image-processing pipeline (R-MOD: Real-time Moving Object Detector) based on deep learning for high-throughput microscopy-based label-free IFC in a microfluidic chip...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911768/natural-isotope-abundance-in-metabolites-techniques-and-kinetic-isotope-effect-measurement-in-plant-animal-and-human-tissues
#13
Illa Tea, Guillaume Tcherkez
The natural isotope abundance in bulk organic matter or tissues is not a sufficient base to investigate physiological properties, biosynthetic mechanisms, and nutrition sources of biological systems. In fact, isotope effects in metabolism lead to a heterogeneous distribution of (2)H, (18)O, (13)C, and (15)N isotopes in metabolites. Therefore, compound-specific isotopic analysis (CSIA) is crucial to biological and medical applications of stable isotopes. Here, we review methods to implement CSIA for (15)N and (13)C from plant, animal, and human samples and discuss technical solutions that have been used for the conversion to CO2 and N2 for IRMS analysis, derivatization and isotope effect measurements...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28910409/risk-of-acute-gastroenteritis-associated-with-human-bocavirus-infection-in-children-a-systematic-review-and-meta-analysis
#14
Ri De, Liying Liu, Yuan Qian, Runan Zhu, Jie Deng, Fang Wang, Yu Sun, Huijin Dong, Liping Jia, Linqing Zhao
Human bocaviruses (HBoVs), which were first identified in 2005 and are composed of genotypes 1-4, have been increasingly detected worldwide in pediatric patients with acute gastroenteritis. To investigate if HBoV infection is a risk factor of acute gastroenteritis in children younger than 5 years old, we searched PubMed, Embase (via Ovid), the Chinese Biomedical Literature Database (CBM), and the Cochrane Library for studies assessing the prevalence of HBoVs in individuals from Oct 25, 2005 to Oct 31, 2016...
2017: PloS One
https://www.readbyqxmd.com/read/28905648/personhood-dementia-policy-and-the-irish-national-dementia-strategy
#15
Niamh Hennelly, Eamon O'Shea
Personhood and its realisation in person-centred care is part of the narrative, if not always the reality, of care for people with dementia. This paper examines how personhood is conceptualised and actualised in Ireland through a content analysis of organisational and individual submissions from stakeholders in the development of the Irish National Dementia Strategy, followed by an examination of the Strategy itself. The organisational submissions are further categorised into dementia care models. A structural analysis of the Strategy examines its principles, actions and outcomes in relation to personhood...
January 1, 2017: Dementia
https://www.readbyqxmd.com/read/28905238/methological-quality-of-systematic-reviews-and-meta-analyses-on-acupuncture-for-stroke-a-review-of-review
#16
REVIEW
Xin-Lin Chen, Chuan-Wei Mo, Li-Ya Lu, Ri-Yang Gao, Qian Xu, Min-Feng Wu, Qian-Yi Zhou, Yue Hu, Xuan Zhou, Xian-Tao Li
OBJECTIVE: To assess the methodological quality of systematic reviews and meta-analyses regarding acupuncture intervention for stroke and the primary studies within them. METHODS: Two researchers searched PubMed, Cumulative index to Nursing and Allied Health Literature, Embase, ISI Web of Knowledge, Cochrane, Allied and Complementary Medicine, Ovid Medline, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Wanfang and Traditional Chinese Medical Database to identify systematic reviews and meta-analyses about acupuncture for stroke published from the inception to December 2016...
September 13, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28903821/age-and-sex-associated-differences-in-phenotypic-and-functional-characteristics-of-peripheral-blood-lymphocytes-in-chimpanzees-pan-troglodytes
#17
Pramod N Nehete, Elizabeth R Magden, Bharti P Nehete, Lawrence E Williams, Christian R Abee, K Jagannadha Sastry
Chimpanzees are the closest phylogenetic relatives to humans, sharing more than 98% genetic sequence identity. These genetic similarities prompted the belief that chimpanzees can serve as an ideal model for human disease conditions and vaccine development. However, in light of the recent NIH decision to phase out biomedical research in chimpanzees and retire NIH-supported chimpanzees, data from the present study will continue to provide value for the care of aged and sick chimpanzees located in zoos, sanctuaries, and primate centers...
September 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28903187/comparative-physiochemical-analysis-of-hydrophobins-produced-in-escherichia-coli-and-pichia-pastoris
#18
Agnes Przylucka, Gunseli Bayram Akcapinar, Klaus Bonazza, Thiago M Mello-de-Sousa, Astrid R Mach-Aigner, Victor Lobanov, Hinrich Grothe, Christian P Kubicek, Erik Reimhult, Irina S Druzhinina
Hydrophobins (HFBs) are small surface-active proteins secreted by filamentous fungi. Being amphiphilic, they spontaneously form layers that convert surfaces from hydrophilic to hydrophobic and vice versa. We have compared properties of the class II HFB4 and HFB7 from Trichoderma virens as produced in Escherichia coli and Pichia pastoris. Since the production in E. coli required denaturation/renaturation steps because of inclusion bodies, this treatment was also applied to HFBs produced and secreted in yeast...
September 4, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28902155/one-step-facile-synthesis-of-aptamer-modified-graphene-oxide-for-highly-specific-enrichment-of-human-a-thrombin-in-plasma
#19
Yuan Xu, Siyuan Tan, Qionglin Liang, Mingyu Ding
The enrichment of low-abundance proteins in complex biological samples plays an important role in clinical diagnostics and biomedical research. This work reports a novel one-step method for the synthesis of aptamer-modified graphene oxide (GO/Apt) nanocomposites, without introducing the use of gold, for the rapid and specific separation and enrichment of human α-thrombin from buffer solutions with highly concentrated interferences. The obtained GO/Apt nanocomposites had remarkable aptamer immobilization, up to 44...
September 13, 2017: Sensors
https://www.readbyqxmd.com/read/28900867/recent-advances-in-the-etiopathogenesis-of-inflammatory-bowel-disease-the-role-of-omics
#20
REVIEW
Eleni Stylianou
Omics technology presents an exciting and timely opportunity to improve our understanding of the molecular etiology and pathogenesis of the group of chronic, heterogeneous, inflammatory disorders that comprise inflammatory bowel disease (IBD). Interest in the use of omics in the biomedical and clinical research communities is gaining pace due to its potential to make huge strides in our understanding of IBD causality, and pathology. Omics-related research also has applicability for biomarker discovery and the development of individualized treatments for patients, termed 'precision medicine'...
September 12, 2017: Molecular Diagnosis & Therapy
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