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https://www.readbyqxmd.com/read/29136827/biobarcode-assay-for-the-oral-anticoagulant-acenocoumarol
#1
Marta Broto, J Pablo Salvador, Roger Galve, M Pilar Marco
A novel approach for therapeutic drug monitoring of oral anticoagulants (OA) in clinical samples is reported, based on a NP-based biobarcode assay. The proposed strategy uses specific antibodies for acenocumarol (ACL) covalently bound to magnetic particles (pAb236-MP) and a bioconjugate competitor (hACL-BSA) linked to encoded polystyrene probes (hACL-BSA-ePSP) on a classical competitive immunochemical format. By using this scheme ACL can be detected in low nM range (LOD, 0.96 ± 0.26, N = 3, in buffer) even in complex samples such as serum or plasma (LOD 4 ± 1)...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135000/bioconjugated-fluorescent-organic-nanoparticles-targeting-egfr-overexpressing-cancer-cells
#2
Adrien Faucon, Houda Benhelli-Mokrani, Fabrice Fleury, Stéphanie Dutertre, Marc Tramier, Joanna Boucard, Lénaïc Lartigue, Steven Nedellec, Philippe Hulin, Eléna Ishow
The field of optical bioimaging has considerably flourished with the advent of sophisticated microscopy techniques and ultra-bright fluorescent tools. Fluorescent organic nanoparticles (FONs) have thus recently appeared as very attractive labels for their high payload, absence of cytotoxicity and eventual biodegradation. Nevertheless, their bioconjugation to target specific receptors with high imaging contrast is scarcely performed. Moreover, assessing the reality of bioconjugation represents high challenges given the sub-nanomolar concentrations resulting from the commonly adopted nanoprecipitation fabrication process...
November 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/29131594/primary-amine-clustered-dna-aptamer-for-dna-protein-conjugation-catalyzed-by-microbial-transglutaminase
#3
Mari Takahara, Rie Wakabayashi, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya
DNA-protein conjugates are promising biomolecules for use in areas ranging from therapeutics to analysis because of the dual functionalities of DNA and protein. Conjugation requires site-specific and efficient covalent bond formation without impairing the activity of both biomolecules. Herein, we have focused on the use of a microbial transglutaminase (MTG) that catalyzes the cross-linking reaction between a glutamine residue and a primary amine. In a model bioconjugation, a highly MTG-reactive Gln (Q)-donor peptide (FYPL<u>Q</u>MRG, FQ) was fused to enhanced green fluorescent protein (FQ-EGFP) and a primary amine-clustered DNA aptamer was enzymatically synthesized as a novel acyl-acceptor substrate of MTG, whose combination leads to efficient and convenient preparation of DNA-protein conjugates with high purity...
November 13, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29129733/doxorubicin-synergism-and-resistance-reversal-in-human-neuroblastoma-be-2-c-cell-lines-an-in-vitro-study-with-dextran-catechin-nanohybrids
#4
Orazio Vittorio, Marion Le Grand, Sami A Makharza, Manuela Curcio, Paola Tucci, Francesca Iemma, Fiore Pasquale Nicoletta, Silke Hampel, Giuseppe Cirillo
Hybrid nanocarrier consisting in nanographene oxide coated by a dextran-catechin conjugate was proposed in the efforts to find more efficient Neuroblastoma treatment with Doxorubicin chemotherapy. The dextran-catechin conjugate was prepared by immobilized laccase catalysis and its peculiar reducing ability exploited for the synthesis of the hybrid carrier. Raman spectra and DSC thermograms were recorded to check the physicochemical properties of the nanohybrid, while DLS measurements, SEM, TEM, and AFM microscopy allowed the determination of its morphological and dimensional features...
November 9, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29126854/biomedical-applications-of-glyconanoparticles-based-on-quantum-dots
#5
REVIEW
C R A Cunha, A D P R Oliveira, T V C Firmino, D P L A Tenório, G Pereira, L B Carvalho, B S Santos, M T S Correia, A Fontes
BACKGROUND: Quantum dots (QDs) are outstanding nanomaterials of great interest to life sciences. Their conjugation versatility added to unique optical properties, highlight these nanocrystals as very promising fluorescent probes. Among uncountable new nanosystems, in the last years, QDs conjugated to glycans or lectins have aroused a growing attention and their application as a tool to study biological and functional properties has increased. SCOPE OF REVIEW: This review describes the strategies, reported in the literature, to conjugate QDs to lectins or carbohydrates, providing valuable information for the elaboration, improvement, and application of these nanoconjugates...
November 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29126850/psma-targeted-bispecific-fab-conjugates-that-engage-t-cells
#6
James T Patterson, Jason Isaacson, Lisa Kerwin, Ghazi Atassi, Rohit Duggal, Damien Bresson, Tong Zhu, Heyue Zhou, Yanwen Fu, Gunnar F Kaufmann
Bioconjugate formats provide alternative strategies for antigen targeting with bispecific antibodies. Here, PSMA-targeted Fab conjugates were generated using different bispecific formats. Interchain disulfide bridging of an αCD3 Fab enabled installation of either the PSMA-targeting small molecule DUPA (SynFab) or the attachment of an αPSMA Fab (BisFab) by covalent linkage. Optimization of the reducing conditions was critical for selective interchain disulfide reduction and good bioconjugate yield. Activity of αPSMA/CD3 Fab conjugates was tested by in vitro cytotoxicity assays using prostate cancer cell lines...
October 6, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29125161/dimer-formation-of-gddo3a-arylsulfonamide-complexes-causes-loss-of-ph-dependency-of-relaxivity
#7
Anja Wacker, Fabio Carniato, Carlos Platas-Iglesias, David Esteban-Gomez, Hans-Jürgen Wester, Lorenzo Tei, Johannes Notni
Gadolinium(iii) complexes with pH-dependent relaxivities have been proposed as responsive magnetic resonance imaging (MRI) contrast agents (CA) for mapping of pH value in living subjects. The latter is clinically relevant because hypoxia-induced reduction of interstitial pH (acidosis) is a hallmark of tumor progression and resistance against chemotherapy. In order to obtain versatile building blocks for integration of a pH-responsive MRI-CA functionality into larger molecular assemblies, such as bioconjugates, micelles or nanoparticles, we equipped the structural motif GdDO3A-ethylene(arylsulfonic acid) with additional carboxylic acid moieties in the aromatic para-position...
November 10, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29120000/water-soluble-and-uv-traceable-isatoic-anhydride-based-reagents-for-bioconjugation
#8
Adam Fessler, Corey Garmon, Thomas Heavey, Anthony Fowler, Craig Ogle
Isatoic anhydride is elaborated to water soluble bioconjugation reagents that gives functionality and water solubility in one self-cleaning step. This new platform offers high atom economy with carbon dioxide being the only byproduct, and is shown to very quickly and efficiently label proteins in bicarbonate buffered solutions.
November 9, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29115133/pursuing-the-promise-of-enzymatic-enhancement-with-nanoparticle-assemblies
#9
James Nicholas Vranish, Mario G Ancona, Scott A Walper, Igor L Medintz
The growing emphasis on green chemistry, renewable resources, synthetic biology, regio-/stereospecific chemical transformations, and nanotechnology for providing new biological products and therapeutics is reinvigorating research into enzymatic catalysis. Although the promise is profound, many complex issues remain to be addressed before this effort will have a significant impact. Prime among these is to combat the degradation of enzymes frequently seen in ex vivo formats following immobilization to stabilize the enzymes for long-term application and to find ways of enhancing their activity...
November 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29111663/mussel-inspired-universal-bioconjugation-of-polydiacetylene-liposome-for-droplet-array-biosensors
#10
Do Hyun Kang, Ho-Sup Jung, Keesung Kim, Jinsang Kim
Most solid-state biosensor platforms require a specific immobilization chemistry and a bioconjugation strategy separately to tether sensory molecules to a substrate and attach specific receptors to the sensory unit, respectively. We developed a mussel-inspired universal conjugation method that enable both surface immobilization and bioconjugation at the same time. By incorporating dopamine or catechol moiety into self-signaling polydiacetylene (PDA) liposomes, the PDA liposomes can be immobilized to a wide range of substrates, without any substrate modification...
November 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29108579/exploiting-the-3-aminopropyltriethoxysilane-aptes-autocatalytic-nature-to-create-bioconjugated-microarrays-on-hydrogen-passivated-porous-silicon
#11
Sidney G Coombs, Sitora Khodjaniyazova, Frank V Bright
Porous silicon (pSi) based microarrays are attractive because pSi: (i) can be modified in many ways, (ii) possesses a high surface area, and (iii) exhibits strong photoluminescence (PL). These characteristics make pSi-based microarrays candidates for a host of applications including sensing, optoelectronic devices, and photodetectors. Microarray fabrication requires a high-throughput approach to produce chemically modified, spatially isolated spots on a particular substrate. The most stable platforms are characterized by covalent attachment to the substrate...
January 15, 2018: Talanta
https://www.readbyqxmd.com/read/29101876/target-triggered-cleavage-effect-of-dnazyme-relying-on-pd-pt-alloys-functionalized-fe-mofs-for-amplified-detection-of-pb-2
#12
Yujie Yu, Chao Yu, Yazhen Niu, Jun Chen, Yilin Zhao, Yuchan Zhang, Rufei Gao, Junlin He
We designed an amplified detection strategy for the sensitive determination of lead ions (Pb(2+)) based on a target-triggered nuclear acid cleavage of Pb(2+)-specific DNAzyme as a selectivity interface combined with Pd-Pt alloys modified Fe-MOFs (Fe-MOFs/PdPt NPs) hybrids acting as the signal tag. Streptavidin modified reduced graphene oxide-tetraethylene pentamine-gold nanoparticles (rGO-TEPA-Au) served as a sensor platform for immobilizing more DNAzyme. In the presence of Pb(2+), the substrate DNA strand can be specifically cleaved at the ribonucleotide site by DNAzyme to produce a new single-DNA on the interface...
October 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29099179/synthesis-and-biological-evaluation-of-ru-ii-and-pt-ii-complexes-bearing-carboxyl-groups-as-potential-anticancer-targeted-drugs
#13
Ma Ángeles Martínez, M Pilar Carranza, Anna Massaguer, Lucia Santos, Juan A Organero, Cristina Aliende, Rafael de Llorens, Iteng Ng-Choi, Lidia Feliu, Marta Planas, Ana M Rodríguez, Blanca R Manzano, Gustavo Espino, Félix A Jalón
The synthesis and characterization of Pt(II) (1 and 2) and Ru(II) arene (3 and 4) or polypyridine (5 and 6) complexes is described. With the aim of having a functional group to form bioconjugates, one uncoordinated carboxyl group has been introduced in all complexes. Some of the complexes were selected for their potential in photodynamic therapy (PDT). The molecular structures of complexes 2 and 5, as well as that of the sodium salt of the 4'-(4-carboxyphenyl)-2,2':6',2″-terpyridine ligand (cptpy), were determined by X-ray diffraction...
November 3, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29097938/labelling-of-90-y-and-177-lu-dota-bioconjugates-for-targeted-radionuclide-therapy-a-comparison-among-manual-semiautomated-and-fully-automated-synthesis
#14
Michele Iori, Pier C Capponi, Sara Rubagotti, Luca Rosario Esposizione, Johanna Seemann, Riccardo Pitzschler, Thorsten Dreger, Debora Formisano, Elisa Grassi, Federica Fioroni, Annibale Versari, Mattia Asti
In spite of the hazard due to the radiation exposure, preparation of (90)Y- and (177)Lu-labelled radiopharmaceuticals is still mainly performed using manual procedures. In the present study the performance of a commercial automatic synthesizer based on disposable cassettes for the labelling of (177)Lu- and (90)Y-DOTA-conjugated biomolecules (namely, DOTATOC and PSMA-617) was evaluated and compared to a manual and a semiautomated approach. The dose exposure of the operators was evaluated as well. More than 300 clinical preparations of both (90)Y- and (177)Lu-labelled radiopharmaceuticals have been performed using the three different methods...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29082104/dramatic-enhancement-of-the-detection-limits-of-bioassays-via-ultrafast-deposition-of-polydopamine
#15
Junwei Li, Madison A Baird, Michael A Davis, Wanyi Tai, Larry S Zweifel, Kristina M Adams Waldorf, Michael Gale, Lakshmi Rajagopal, Robert H Pierce, Xiaohu Gao
The ability to detect biomarkers with ultrahigh sensitivity radically transformed biology and disease diagnosis. However, owing to incompatibilities with infrastructure in current biological and medical laboratories, recent innovations in analytical technology have not received broad adoption. Here, we report a simple, universal 'add-on' technology (dubbed EASE) that can be directly plugged into the routine practices of current research and clinical laboratories and that converts the ordinary sensitivities of common bioassays to extraordinary ones...
2017: Nature biomedical engineering
https://www.readbyqxmd.com/read/29081048/solid-binding-peptides-in-biomedicine
#16
Andrew Care, Peter L Bergquist, Anwar Sunna
Some peptides are able to bind to inorganic materials such as silica and gold. Over the past decade, Solid-binding peptides (SBPs) have been used increasingly as molecular building blocks in nanobiotechnology. These peptides show selectivity and bind with high affinity to a diverse range of inorganic surfaces e.g. metals, metal oxides, metal compounds, magnetic materials, semiconductors, carbon materials, polymers and minerals. They can be used in applications such as protein purification and synthesis, assembly and the functionalization of nanomaterials...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29077399/last-step-pd-mediated-11-c-co-labeling-of-a-moxestrol-conjugated-o-iodobenzyl-alcohol-from-model-experiments-to-in-vivo-positron-emission-tomography-studies
#17
Thomas Cornilleau, Mette Simonsen, Maylou Vang, Nada Taib-Maamar, Jean Dessolin, Hélène Audrain, Philippe Hermange, Eric Fouquet
The fast, efficient, and functional group tolerant last-step radiolabeling of bioconjugates is crucial for positron emission tomography (PET) applications. In this context, o-iodobenzyl alcohol based structures were identified as ideal tags for an easy Pd-catalyzed carbonylation after bioconjugation, and a moxestrol-conjugated precursor was chosen as the model compound for the further studies. Despite scale and time constraints, conditions developed with [(12)C]CO and [(13)C]CO were easily transferred to the (11)C isotope, and the desired radioactive product was obtained in amounts up to 740 MBq with radiochemical purities higher than 99%...
October 27, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29077116/structural-investigation-of-cyclo-dioxo-maleimide-cross-linkers-for-acid-and-serum-stability
#18
Elisabetta Tobaldi, Igor Dovgan, Michel Mosser, Jean-Michel Becht, Alain Wagner
The biochemical characteristics of hetero-bifunctional cross-linkers used in bioconjugates are of essential importance to the desired features of the final adduct (i.e. antibody-drug conjugates). These include stability in biological media, chemical and biological reactivities, cleavability under defined conditions, and solubility. In our previous work, we introduced a new amino-to-thiol linker, maleimidomethyl dioxane (MD), as an alternative to classical maleimide conjugation, with increased hydrophilicity and serum stability due to succinimidyl ring-opening...
November 15, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29067569/anti-tumor-study-of-chondroitin-sulfate-methotrexate-nanogels
#19
Jinyu Wang, Weibo Zhao, Haixiao Chen, An Qin, Peizhi Zhu
Self-assembly nanogels (NGs) were formed by bioconjugating methotrexate (MTX) with chondroitin sulfate (CS). MTX-CS NGs can greatly enhance the solubility and improve the delivery efficacy of MTX due to the CD44 binding property of CS. Vivo experiments revealed that MTX-CS NGs showed less toxicity than MTX. MTX-CS NGs can improve the anti-tumor effect while reducing the side effects of MTX. Due to their CD44 binding property, chondroitin sulfate-drug conjugates could be a promising and efficient platform for improving the solubility of sparingly soluble drug molecules as well as targeted delivery to cancer cells and tumor tissues...
October 24, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29062164/bioconjugated-graphene-oxide-based-raman-probe-for-selective-identification-of-skbr3-breast-cancer-cells
#20
Afua A Antwi-Boasiako, Derrick Dunn, Samuel S R Dasary, Yolanda K Jones, Sandra L Barnes, Anant K Singh
In this article, we demonstrate the use of bio-conjugated 2D graphene oxide (bio-GO) nanostructure to probe breast cancer cell (SKBR3) with excellent discrimination over other types of circulating tumor cells. We distinctly observed that bio-GO nanostructure targets and bind SKBR3 cell selectively in the cell mixture. Longer incubation of SKBR3 cell with bio-GO causes Raman signal "turn off" when excited with 532 nm laser. This is attributed to penetration of the bio-GO through the plasma membrane of the cell by generating transient hole...
August 2017: Journal of Raman Spectroscopy: JRS
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