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Satish K Tuteja, Todd Duffield, Suresh Neethirajan
We report the step-by-step fabrication of a 2D MoS2 nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS2 nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS2-modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS2 nanosheets...
July 21, 2017: Nanoscale
Jiao Feng, Sinda Lepetre-Mouelhi, Patrick Couvreur
This article reviews the innovative and original concept the "squalenoylation", a technology allowing the formulation of a wide range of drug molecules (both hydrophilic and lipophilic) as nanoparticles. The "squalenoylation" approach is based on the covalent linkage between the squalene, a natural and biocompatible lipid belonging to the terpenoid family, and a drug, in order to increase its pharmacological efficacy. Fundamentally, the dynamically folded conformation of squalene triggers the resulting squalene-drug bioconjugates to self-assemble as nanoparticles of 100-300 nm...
July 19, 2017: Current Topics in Medicinal Chemistry
Eric Waidely, Abdulrahman Obaid Al-Youbi, Abdulaziz Saleh Omar Bashammakh, Mohammad Soror El-Shahawi, Roger M Leblanc
Detection of alpha-L-fucosidase has been shown to have relevance in diagnosing hepatocellular carcinoma. Few assays have been developed to measure this enzyme, with most relying on colorimetric techniques involving the enzyme's kinetics. While these assays are facile and quick, the sensitivity is not always sufficient for early tumor detection. To improve upon previous assays for alpha-L-fucosidase, a fluorescence based immunoassay was produced implementing an alpha-L-fucosidase specific antibody (FUCA2). The immobilization of the alpha-L-fucosidase-specific antibody onto a quartz slide was investigated with several bioconjugation approaches and an immunoassay for detection of alpha-L-fucosidase was produced...
July 20, 2017: Analytical Chemistry
Bindu Y Srinivasu, Beena Bose, Gopa Mitra, Anura V Kurpad, Amit Kumar Mandal
In general, proteins in the biological system interact with nanoparticles (NPs) via adsorption on the particle surface. Understanding the adsorption at molecular level is crucial to explore NP-protein interactions. The increasing concerns about the risk to human health on NP exposure have been explored through the discovery of a handful protein biomarkers and biochemical analysis. However, detailed information on structural perturbation and associated functional changes of proteins on interaction with NPs is limited...
July 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Rohini Singh, Smita Patil, Neetu Singh, Shalini Gupta
Bacterial drug resistance has emerged as a serious global threat mandating the development of novel methodologies that allow facile modulation of antimicrobial action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to meet this goal by increasing the drug's overall avidity, bioavailability and easier internalisation into mammalian cells, targeting bacteria that otherwise escape antibacterial action by host cell-localisation. We used polymyxin B sulfate (PMB) and sushi peptide as model drugs against Gram-negative bacteria and established their enhanced antimicrobial activity on Escherichia coli (E...
July 19, 2017: Scientific Reports
Tianzhi Luo, Michael A David, Lucas C Dunshee, Rebecca A Scott, Morgan A Urello, Christopher Price, Kristi L Kiick
Over the past few decades, (poly)peptide block copolymers have been widely employed in generating well-defined nanostructures as vehicles for targeted drug delivery applications. We previously reported the assembly of thermoresponsive nanovesicles from an elastin-b-collagen like peptide (ELP-CLP). The nanoparticles were observed to dissociate at elevated temperatures, despite the LCST-like behavior of the tethered ELP domain, which is suggested to be triggered by the unfolding of the CLP domain. Here, the potential of using the nanoparticles as drug delivery vehicles for targeting collagen-containing matrices is evaluated...
July 18, 2017: Biomacromolecules
Peng Zhao, Quan Xu, Jia Tao, Zongwen Jin, Yue Pan, Changmin Yu, Zhiqiang Yu
To address the requirements of biomedical applications including biosensing, bioimaging, and drug delivery, fluorescent nanomaterials served as efficient tools in many cases. Among them, near-infrared quantum dots (NIR QDs) have been used as novel fluorescent labels for their binary advantages of both QDs and NIR light. In this review, through collecting references in recent 10 years, we have introduced basic structures and properties of NIR QDs and summarized the classification and the related synthetic methods...
July 18, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Utkarsh Jain, Shaivya Gupta, Nidhi Chauhan
The glucose level measurement in the diabetic patient plays a vital role in identification of the treatments going on and it also provides the control over the diabetics. A new electrochemical sensing device was constructed for determination of glycated hemoglobin (HbA1c) in whole blood samples. Fructosyl amine oxidase (FAO) was bioconjugated onto hybrid nanocomposite i.e., gold nanoparticles-platinum nanoparticles (AuNPs-PtNPs) and poly indole-5-carboxylic acid (PIN5COOH), deposited electrochemically on gold electrode...
July 14, 2017: International Journal of Biological Macromolecules
Rafael Del Caño, Lucia Mateus, Guadalupe Sánchez-Obrero, José Manuel Sevilla, Rafael Madueño, Manuel Blázquez, Teresa Pineda
The identification of the factors that dictate the formation and physicochemical properties of protein-nanomaterial bioconjugates are important to understand their behavior in biological systems. The present work deals with the formation and characterization of bioconjugates made of the protein hemoglobin (Hb) and gold nanoparticles (AuNP) capped with three different molecular layers (citrate anions (c), 6-mercaptopurine (MP) and ω-mercaptoundecanoic acid (MUA)). The main focus is on the behavior of the bioconjugates in aqueous buffered solutions in a wide pH range...
July 5, 2017: Journal of Colloid and Interface Science
Uwe Seibold, Björn Wängler, Carmen Wängler
89Zr is a positron-emitting radionuclide being of high interest for medical imaging applications with Positron Emission Tomography. For the introduction of this radiometal into biologically active targeting vectors, the chelating agent desferrioxamine B (DFO) is commonly applied which is however known to form 89Zr-complexes of limited in vivo stability. We herein describe the rational design and chemical development a new, macrocyclic, four hydroxamates-bearing chelating agent (CTH36) for the stable complexation of Zr4+...
July 17, 2017: ChemMedChem
Ramesh Dasari, James J La Clair, Alexander Kornienko
The application of new chemical reactions in a biological context has advanced bioconjugation methods for both fundamental research and commercial arenas. Recent adaptations of reactions such as Huisgen 1,3-dipolar or Diels-Alder cycloadditions have enabled the labeling of specific residues in biomolecules by the attachment of molecules carrying azides, alkynes, or strained alkenes. Although these are fundamental tools, there is a need for the discovery of reactions that can label native proteins. We now report on the adaptation of the Paal-Knorr reaction to label lysine-residues in proteins via pyrrole linkages...
July 17, 2017: Chembiochem: a European Journal of Chemical Biology
Hema Krishnan, Longle Ma, Neil Vasdev, Steven Liang
Positron emission tomography (PET) imaging study of 18F-labeled biomolecules is an emerging and rapidly growing area for preclinical and clinical research. The present review focuses on recent advances in radiochemical methods for incorporating 18F into biomolecules via 'direct' or 'indirect' bioconjugation. Recently developed prosthetic groups and pre-targeting strategies, as well as representative examples in 18F-labeling of biomolecules in PET imaging research studies are highlighted.
July 13, 2017: Chemistry: a European Journal
J D Castillo Gomez, A Hagenbach, U I M Gerling-Driessen, B Koksch, N Beindorff, W Brenner, U Abram
Potential tetradentate thiocarbamoylbenzamidine derivatives H4L have been synthesized from the corresponding benzimidoyl chlorides and triglycine. They are suitable chelating agents for the oxidotechnetium(v) and oxidorhenium(v) cores and form stable, neutral [MO(HL)] complexes with an equatorial SN3 coordination sphere and an additional, uncoordinated carboxylic group, which can be used for bioconjugation. Representatives of the rhenium and (99)Tc products have been isolated and analyzed with spectroscopic methods and X-ray diffraction...
July 12, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Karla Alejo-González, Katrin Quester, Erik Hanson, Ismael Secundino, Yvonne Rosenstein, Alejandro Huerta-Saquero, Rafael Vazquez-Duhalt
Pesticide intoxication is a major public health concern, and unfortunately there is not an effective treatment for severe organophosphorus pesticide intoxication. In this work, a non-immunogenic enzymatic bioconjugate based on cytochrome P450 was assayed for organophosphorus pesticide transformation. Enzyme therapy is an alternative approach to inactivate pesticides in the bloodstream, transforming them into less toxic metabolites. A variant of cytochrome P450 (CYPBM3 F87A) from Bacillus megaterium was chemically modified with polyethylene glycol...
July 6, 2017: International Journal of Biological Macromolecules
Wing Ho So, Yu Zhang, Wei Kang, Clarence T T Wong, Hongyan Sun, Jiang Xia
To achieve precise control of the signaling events or to achieve unmistakable synthesis of biomolecules, nature has evolved organic reactions involving proteinogenic amino acids with unparalleled site selectivity. For example, dedicated enzymes accurately dictate the site of post-translational modifications in signaling proteins, and ribosomes precisely link the C-terminal carboxylic acid of one unprotected amino acid with the N-terminal amino group of the other amino acid through spatially confined proximity...
July 5, 2017: Current Opinion in Biotechnology
Anthony Paul Silvestri, Philip A Cistrone, Philip E Dawson
The copper-mediated coupling between alkynes to generate a structurally efficient, linear 1,3-diyne linkage has been known for over a century. However, the mechanistic requirement for simultaneous Cu(I) and an oxidant has limited its practical utility, especially for complex functional molecules in aqueous solution. We find that addition of a specific bpy-diol ligand protects unprotected peptides from Cu(II) mediated oxidative damage through formation of an insoluble Cu(II) gel which solves the critical challenge of applying the Glaser coupling to substrates that are degraded by Cu(II)...
July 6, 2017: Angewandte Chemie
Monika Lyczko, Marek Pruszynski, Agnieszka Majkowska-Pilip, Krzysztof Lyczko, Bogdan Was, Sylwia Meczynska-Wielgosz, Marcin Kruszewski, Katarzyna Szkliniarz, Jerzy Jastrzebski, Anna Stolarz, Aleksander Bilewicz
INTRODUCTION: The purposes of the present work were to label substance P (5-11) with (211)At using a rhodium(III) complex with a bifunctional ligand-2-(1,5,9,13-tetrathiacyclohexadecan-3-yloxy)acetic acid ([16aneS4]-COOH) and to assess the in vitro stability and toxicity of the obtained radiobioconjugate. METHODS: Two approaches were evaluated to obtain (131)I/(211)At-Rh[16aneS4]-SP5-11 radiobioconjugates, based on 2-step and 1-step syntheses. In the first method (131)I/(211)At-Rh[16aneS4]-COOH complexes were obtained that required further coupling to a biomolecule...
May 25, 2017: Nuclear Medicine and Biology
Mads Westergaard Mulberg, Maria Taskova, Rasmus P Thomsen, Anders H Okholm, Jørgen Kjems, Kira Astakhova
For decades the detection of nucleic acids and their interactions at low abundances has been a challenging task that has thus far been solved by enzymatic target amplification. In this work we aimed at developing efficient tools for amplification-free nucleic acid detection, which resulted in the synthesis of new fluorescent nanoparticles. Here, the fluorescent nanoparticles were made by simple and inexpensive radical emulsion polymerization of butyl acrylate in the presence of fluorescent dyes and additional functionalization reagents...
July 6, 2017: Chembiochem: a European Journal of Chemical Biology
NIcholas T Jacob, Kensaku Anraku, Atsushi Kimishima, Bin Zhou, Karen C Collins, Jonathan W Lockner, Beverley A Ellis, Kim D Janda
A method for potentiating the response to an anti-cocaine vaccine by leveraging xenoreactive antibodies against the carbohydrate epitope Galα1,3-Gal (GAL) was found to result in a highly specific anti-cocaine response that was able to significantly attenuate cocaine-induced locomotion at 20 mg kg(-1) with superior efficacy compared to a standard conjugate.
July 18, 2017: Chemical Communications: Chem Comm
Robert Walder, Marc-Andre LeBlanc, William John Van Patten, Devin Edwards, Jacob A Greenberg, Ayush Adhikari, Stephen R Okoniewski, Ruby May Arana Sullan, David Rabuka, Marcelo Carlos Sousa, Thomas T Perkins
Atomic-force-microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is a powerful yet accessible means to characterize mechanical properties of biomolecules. Historically, accessibility relies upon the nonspecific adhesion of biomolecules to a surface and a cantilever and, for proteins, the integration of the target protein into a polyprotein. However, this assay results in a low yield of high-quality data, defined as the complete unfolding of the polyprotein. Additionally, nonspecific surface adhesion hinders studies of α-helical proteins, which unfold at low forces and low extensions...
July 5, 2017: Journal of the American Chemical Society
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