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https://www.readbyqxmd.com/read/29760220/quantifying-atherogenic-lipoproteins-current-and-future-challenges-in-the-era-of-personalized-medicine-and-vldl-cholesterol-a-consensus-statement-from-eas-and-eflm
#1
Michel R Langlois, M John Chapman, Christa Cobbaert, Samia Mora, Alan T Remaley, Emilio Ros, Gerald F Watts, Jan Borén, Hannsjörg Baum, Eric Bruckert, Alberico Catapano, Olivier S Descamps, Arnold von Eckardstein, Pia R Kamstrup, Genovefa Kolovou, Florian Kronenberg, Anne Langsted, Kari Pulkki, Nader Rifai, Grazyna Sypniewska, Olov Wiklund, B Rge G Nordestgaard
BACKGROUND: The European Atherosclerosis Society-European Federation of Clinical Chemistry and Laboratory Medicine Consensus Panel aims to provide recommendations to optimize atherogenic lipoprotein quantification for cardiovascular risk management. CONTENT: We critically examined LDL cholesterol, non-HDL cholesterol, apolipoprotein B (apoB), and LDL particle number assays based on key criteria for medical application of biomarkers. ( a ) Analytical performance: Discordant LDL cholesterol quantification occurs when LDL cholesterol is measured or calculated with different assays, especially in patients with hypertriglyceridemia >175 mg/dL (2 mmol/L) and low LDL cholesterol concentrations <70 mg/dL (1...
May 14, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29757133/haemolysis-index-for-the-screening-of-intravascular-haemolysis-a-novel-diagnostic-opportunity
#2
REVIEW
Giuseppe Lippi, Emmanuel J Favaloro, Massimo Franchini
The diagnostic approach to patients with intravascular haemolysis remains challenging, since no first-line laboratory test seems to be entirely suitable for the screening of this condition. Recent evidence shows that an enhanced cell-free haemoglobin (fHb) concentration in serum or plasma is a reliable marker of red blood cell injury, and may also predict clinical outcomes in patients with different forms of haemolytic anaemias. However, the routine use of the haemiglobincyanide assay, the current reference method for measuring fHb, seems unsuitable for a timely diagnosis of intravascular haemolysis, for many safety and practical reasons...
May 8, 2018: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/29756761/digital-microarrays-single-molecule-readout-with-interferometric-detection-of-plasmonic-nanorod-labels
#3
Derin Sevenler, George G Daaboul, Fulya Ekiz Kanik, Nese Lortlar Ünlü, M Selim Ünlu
DNA and protein microarrays are a high-throughput technology that allow the simultaneous quantification of tens of thousands of different biomolecular species. The mediocre sensitivity and limited dynamic range of traditional fluorescence microarrays compared to other detection techniques have been the technology's Achilles' Heel, and prevented their adoption for many biomedical and clinical diagnostic applications. Previous work to enhance the sensitivity of microarray readout to the single-molecule ('digital') regime have either required signal amplifying chemistry or sacrificed throughput, nixing the platform's primary advantages...
May 14, 2018: ACS Nano
https://www.readbyqxmd.com/read/29738695/an-approach-to-analytical-validation-and-testing-of-body-fluid-assays-for-the-automated-clinical-laboratory
#4
Darci R Block, Lucas J Ouverson, Craig A Wittwer, Amy K Saenger, Nikola A Baumann
BACKGROUND: Biochemical analysis of body fluids may lend insight into the pathogenesis of disease. Most commercially-available clinical laboratory methods are intended for blood-derived specimens and/or urine and laboratories must characterize the analytical performance of these methods for other specimen types such as body fluids. The aim of this work is to demonstrate one approach for characterizing analytical performance of assays for clinical analysis of body fluids. METHODS: Residual waste samples were obtained from clinically ordered testing...
May 5, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29735799/therapeutic-effect-of-hgf-on-nash-mice-through-hgf-c-met-and-jak2-stat3-signalling-pathway
#5
Ning Li, Zhangfeng Dou, Jinchun Liu, Bao Chai, Yue Li, Xiuqin An, Peiling Chu, Xiaolan Zhang
INTRODUCTION AND AIM: Hepatocyte growth factor (HGF) has been shown to ameliorate liver inflammation and fibrosis; however, the mechanism underlying its effects in non-alcoholic steatohepatitis (NASH) is unclear. This study aimed to analyse the relationship between the JAK2-STAT3 signalling pathway and the ameliorating effect of HGF on NASH. MATERIAL AND METHODS: Mice were fed a high-fat diet (HFD) for 16 weeks, and then plasma and hepatic tissues were collected...
April 9, 2018: Annals of Hepatology
https://www.readbyqxmd.com/read/29709451/development-of-an-enzymatic-assay-to-measure-lactate-in-perchloric-acid-precipitated-cerebrospinal-fluid
#6
Jun Lu, Jonathan R Genzen, David G Grenache
BACKGROUND: Individuals with inherited deficiencies of the pyruvate dehydrogenase complex or the respiratory chain complex can have increased concentrations of cerebrospinal fluid (CSF) lactate. Such measurements are clinical useful when measured in conjunction with pyruvate in order to calculate the lactate:pyruvate (L:P) ratio, a useful surrogate of cytosolic redox status. CSF pyruvate is measured in a protein-free supernatant prepared by the addition of CSF to perchloric acid while lactate is measured in untreated CSF...
April 27, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29692587/sigma-metrics-as-a-tool-for-evaluating-the-performance-of-internal-quality-control-in-a-clinical-chemistry-laboratory
#7
B Vinodh Kumar, Thuthi Mohan
OBJECTIVE: Six Sigma is one of the most popular quality management system tools employed for process improvement. The Six Sigma methods are usually applied when the outcome of the process can be measured. This study was done to assess the performance of individual biochemical parameters on a Sigma Scale by calculating the sigma metrics for individual parameters and to follow the Westgard guidelines for appropriate Westgard rules and levels of internal quality control (IQC) that needs to be processed to improve target analyte performance based on the sigma metrics...
April 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/29657831/pharmacodynamic-and-pharmacokinetic-effects-and-safety-of-verinurad-in-combination-with-febuxostat-in-adults-with-gout-a-phase-iia-open-label-study
#8
Roy Fleischmann, Peter Winkle, Jesse Hall, Shakti Valdez, Sha Liu, Xiaohong Yan, Liz Hicks, Caroline Lee, Jeffrey N Miner, Michael Gillen, Martha Hernandez-Illas
Objective: Verinurad (RDEA3170) is a high-affinity, selective URAT1 inhibitor in development for treating gout and asymptomatic hyperuricaemia. This study evaluated the pharmacodynamics, pharmacokinetics and safety of verinurad in combination with febuxostat in adults with gout. Methods: The phase IIa, open-label, multicentre study randomised 64 subjects into one of five cohorts to receive febuxostat (40 or 80 mg) alone or in combination with verinurad 2.5-20 mg...
2018: RMD Open
https://www.readbyqxmd.com/read/29614248/comparative-testing-of-hpv-l1-protein-monoclonal-antibody-panel-for-the-detection-of-hpv-in-cervical-exfoliated-cells
#9
Le Wang, Yu-Chang Hu, Chang-Yi Xiao, Fei Wang, Yu-Fei Liu, Li-Hua Tang, Rong-Shuang Xiao
AIMS: To determine the value of a monoclonal antibody panel against a C-terminal conserved sequence polypeptide of human papillomavirus (HPV) L1 (a major capsid protein) for the detection of HPV in cervical exfoliated cells, as well as the potential of this antibody panel to be developed into an assay kit for the clinical screening of cervical cancer. METHODS: Cervical exfoliated cells were collected at a gynecology clinic. One part of each sample was sent to the Department of Pathology for HPV genotyping, and the other part was sent to the Department of Pathology for cytologic testing and then to the laboratory for immunological histological chemistry (IHC) assay in which an HPV L1 C-terminal conserved sequence polypeptide-induced mouse monoclonal antibody panel was used to detect HPV L1...
March 31, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29599804/metabolic-profiling-analysis-of-the-alleviation-effect-of-the-fractions-of-niuhuang-jiedu-tablet-on-realgar-induced-toxicity-in-rats
#10
Wenfeng Xu, Yuehu Pei, Shuo Xu, Haifeng Wang, Pengfei Jin
Niuhuang Jiedu Tablet (NJT) is a classical formula in treating acute tonsillitis, pharyngitis, and so on. In the formula, significant level of Realgar as a potentially toxic element is contained. Our previous experiments revealed that it was less toxic for combined Realgar in NJT. However, the active fraction of this prescription with toxicity alleviation effect on Realgar was still obscure. NJT was divided into five different polar fractions (NJT-PET, NJT-25, NJT-50, NJT-75, and NJT-95), and we explored the toxicity alleviation effect on Realgar ...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29596792/first-two-reagent-vitamin-d-assay-for-general-clinical-chemistry
#11
Fakhri B Saida, Mario Padilla-Chee, Chao Dou, Chong Yuan
BACKGROUND: Vitamin D is a lipid-soluble molecule that plays key physiological roles in the metabolism of calcium, phosphate and magnesium. Recent studies show that deficiency in vitamin D is linked to cardiovascular diseases, autoimmune diseases and cancer. As a result, regular monitoring of 25-OH vitamin D (the main circulating form of vitamin D) is becoming essential. Current 25-OH vitamin D testing methodologies are cumbersome (too many reagents, long incubation times, phase separation) and are not compatible with general clinical chemistry platforms...
March 26, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29595947/unique-fluorescent-probe-molecule-for-bacterial-surface-localization-and-resistant-enzyme-imaging
#12
Hui Ling Chan, Linna Lyu, Junxin Aw, Wenmin Zhang, Juan Li, Huang-Hao Yang, Hirohito Hayashi, Shunsuke Chiba, Bengang Xing
Emergence of antibiotic bacterial resistance has caused serious clinical issues worldwide due to increasingly difficult treatment. Development of a specific approach for selective visualization of resistant bacteria will be highly significant for clinical investigations to promote timely diagnosis and treatment of bacterial infections. In this article, we present an effective method that not only is able to selectively recognize drug resistant AmpC β-lactamases enzyme, but more importantly, is able to interact with bacterial cell wall components resulting in a desired localization effect on the bacterial surface...
March 29, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29580857/analytical-interference-of-hboc-201-hemopure-a-synthetic-hemoglobin-based-oxygen-carrier-on-four-common-clinical-chemistry-platforms
#13
Erik A Korte, Nicole Pozzi, Nina Wardrip, M Tayyeb Ayyoubi, Saeed A Jortani
BACKGROUND: There are 13 million blood transfusions each year in the US. Limitations in the donor pool, storage capabilities, mass casualties, access in remote locations and reactivity of donors all limit the availability of transfusable blood products to patients. HBOC-201 (Hemopure®) is a second-generation glutaraldehyde-polymer of bovine hemoglobin, which can serve as an "oxygen bridge" to maintain oxygen carrying capacity while transfusion products are unavailable. Hemopure presents the advantages of extended shelf life, ambient storage, and limited reactive potential, but its extracellular location can also cause significant interference in modern laboratory analyzers similar to severe hemolysis...
March 23, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29545112/validation-conform-iso-15189-of-assays-in-the-field-of-autoimmunity-joint-efforts-in-the-netherlands
#14
REVIEW
Leontine Mulder, Renate van der Molen, Carin Koelman, Ester van Leeuwen, Anja Roos, Jan Damoiseaux
ISO 15189:2012 requires validation of methods used in the medical laboratory, and lists a series of performance parameters for consideration to include. Although these performance parameters are feasible for clinical chemistry analytes, application in the validation of autoimmunity tests is a challenge. Lack of gold standards or reference methods in combination with the scarcity of well-defined diagnostic samples of patients with rare diseases make validation of new assays difficult. The present manuscript describes the initiative of Dutch medical immunology laboratory specialists to combine efforts and perform multi-center validation studies of new assays in the field of autoimmunity...
May 2018: Autoimmunity Reviews
https://www.readbyqxmd.com/read/29536424/effect-of-socio-demographic-factors-on-endogenous-biomarkers-cystatin-c-and-creatinine-among-elderly-chronic-kidney-disease-patients-a-cross-sectional-study
#15
Irfanullah Khan, Amer Hayat Khan, Azreen Syazril Adnan, Syed Azhar Syed Sulaiman, Azhar Bin Amir Hamzah, Nafees Ahmed, Amjad Khan
PURPOSE: Creatinine is normally used to evaluate kidney function among elderly patients in clinical practice, which has been reported to be affected by socio-demographic factors like BMI and age. Cystatin C a newly introduced biomarker may be more efficient in identifying kidney function in obese and aged CKD patients. The aim of the current study was to assess the effect of BMI on endogenous biomarkers (cystatin C and creatinine) among elderly CKD patients in Malaysia, a first such study in the country...
March 13, 2018: International Urology and Nephrology
https://www.readbyqxmd.com/read/29528429/comparison-of-enzymatic-assay-for-hba1c-measurement-abbott-architect-with-capillary-electrophoresis-sebia-minicap-flex-piercing-analyser
#16
Andrea Tesija Kuna, Kristina Dukic, Nora Nikolac Gabaj, Marijana Miler, Ines Vukasovic, Sanja Langer, Ana-Maria Simundic, Nada Vrkic
Objective: To compare the analytical performances of the enzymatic method (EM) and capillary electrophoresis (CE) for hemoglobin A1c (HbA1c) measurement. Methods: Imprecision, carryover, stability, linearity, method comparison, and interferences were evaluated for HbA1c via EM (Abbott Laboratories, Inc) and CE (Sebia). Results: Both methods have shown overall within-laboratory imprecision of less than 3% for International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) units (<2% National Glycohemoglobin Standardization Program [NGSP] units)...
March 8, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29493873/rapid-detection-of-copper-in-biological-systems-using-click-chemistry
#17
Wenshu Zheng, Huiling Li, Wenwen Chen, Jiangjiang Zhang, Nuoxin Wang, Xuefeng Guo, Xingyu Jiang
A fast (1 min), straightforward but efficient, click chemistry-based system that enables the rapid detection of free copper (Cu) ions in either biological fluids or living cells without tedious pretreatment is provided. Cu can quickly induce the conjugation between graphene oxide (GO) and a fluorescent dye via click reaction. On the basis of the high specificity of bioorthogonal reaction and the effective quenching ability of GO, the assay studied in this paper can respond to Cu ions in less than 1 min with excellent selectivity and sensitivity, which is the fastest sensor for Cu as far as it is known...
April 2018: Small
https://www.readbyqxmd.com/read/29480958/controllable-assembly-of-enzymes-for-multiplexed-lab-on-a-chip-bioassays-with-a-tunable-detection-range
#18
Yunlei Xianyu, Jing Wu, Yiping Chen, Wenshu Zheng, Mengxia Xie, Xingyu Jiang
Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab-on-a-chip immunoassays with a tunable detection range from pg mL-1 to μg mL-1 . Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab-on-a-chip format, with a limit of detection of 0...
February 26, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29479869/development-of-a-strategy-based-on-the-surface-plasmon-resonance-technology-for-platelet-compatibility-testing
#19
Chang-Lin Wu, Jian-An He, Da-Yong Gu, Chao-Peng Shao, Yi Zhu, Xin-Tang Dang
BACKGROUND: This study was aimed to establish a novel strategy based on the surface plasmon resonance (SPR) technology for platelet compatibility testing. METHODS: A novel surface matrix was prepared based on poly (OEGMA-co-HEMA) via surface-initiated polymerization as a biosensor surface platform. Type O universal platelets and donor platelets were immobilized on these novel matrices via amine-coupling reaction and worked as a capturing ligand for binding the platelet antibody...
January 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29478817/functional-assessment-of-creatine-transporter-in-control-and-x-linked-slc6a8-deficient-fibroblasts
#20
Marie Joncquel-Chevalier Curt, Marie-Adélaïde Bout, Monique Fontaine, Isabelle Kim, Guillemette Huet, Soumeya Bekri, Gilles Morin, Stéphanie Moortgat, Alexandre Moerman, Jean-Marie Cuisset, David Cheillan, Joseph Vamecq
Creatine transporter is currently the focus of renewed interest with emerging roles in brain neurotransmission and physiology, and the bioenergetics of cancer metastases. We here report on amendments of a standard creatine uptake assay which might help clinical chemistry laboratories to extend their current range of measurements of creatine and metabolites in body fluids to functional enzyme explorations. In this respect, short incubation times and the use of a stable-isotope-labeled substrate (D3 -creatine) preceded by a creatine wash-out step from cultured fibroblast cells by removal of fetal bovine serum (rich in creatine) from the incubation medium are recommended...
April 2018: Molecular Genetics and Metabolism
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