Read by QxMD icon Read

Viral genotyping

Chung-Feng Huang, Shu-Chi Wang, Ming-Lun Yeh, Ching-I Huang, Pei-Chien Tsai, Zu-Yau Lin, Shinn-Cherng Chen, Chia-Yen Dai, Jee-Fu Huang, Wan-Long Chuang, Angela Chen, Ming-Lung Yu
BACKGROUND/AIMS: MHC class I chain-related A (MICA) genetic variants and their serum levels (sMICA) were associated with the development of hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC) in untreated cohorts. The dynamic changes in serial sMICA levels and their association with HCC in the post-curative status is elusive. METHODS: Single nucleotide polymorphism rs2596542 of MICA and serial sMICA levels were analyzed in chronic hepatitis C (CHC) patients with a sustained virologic response after antivirals...
June 22, 2018: Journal of Gastroenterology and Hepatology
M Y W Wong, B Wang, A Yang, A Khor, W Xuan, S Rajendra
Given the comparable strains of high-risk human papillomavirus (HPV) present in a subset of Barrett's dysplasia and esophageal adenocarcinoma as in head and neck squamous cell carcinomas and the anatomical proximity of both lesions, we hypothesized that oral sex may increase the risk of Barrett's dysplasia/esophageal adenocarcinoma. Therefore, we compared the sexual behavior of patients with Barrett's dysplasia/esophageal adenocarcinoma and controls (hospital, reflux, and Barrett's metaplasia) to explore a plausible mechanism of viral transmission to the lower esophagus...
June 21, 2018: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
Samuel J Rednor, Michael J Ross
HIV-associated nephropathy (HIVAN) is an important cause of secondary focal glomerulosclerosis that occurs primarily in persons of African ancestry with advanced HIV disease. Although HIVAN is characterized by severe proteinuria and rapid progression to end stage renal disease without treatment, the phenotype is markedly attenuated by treatment with antiretroviral medications. HIV infection of glomerular and tubular epithelial cells and subsequent viral gene expression is a key contributor to HIVAN pathogenesis and the kidney can serve as reservoir for HIV strains that differ those in blood...
2018: Frontiers in Medicine
Rebecca Scherzer, Mitchell H Grayson
OBJECTIVE: It is a well-known fact that there is significant heterogeneity in the origins of asthma in adults and children. This article examines the roots of asthma across the ages including atopy, the role of the microbiome and viral infections, along with comorbidities/confounders such as obesity, aspirin-exacerbated respiratory disease (AERD), neutrophilic asthma, cigarette smoking and the possibility of an asthma-chronic obstructive pulmonary disease (COPD) overlap syndrome. DATA SOURCES: Data was taken from various scientific search engines including PubMed and Science Direct databases...
June 18, 2018: Annals of Allergy, Asthma & Immunology
D Tasic, I Lazarevic, A Knezevic, L Tasic, A Pikula, Z Perisic, T Jovanovic, M Cupic
Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk-HPV (HR-HPV) is necessary but not a sufficient aetiological agent for the development of cervical neoplasia. A number of viral, host, environmental and behavioural factors are suggested to be associated with the progression of cervical disorder. This study aimed to evaluate the impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia. A total of 541 women have been tested by PCR for the presence of HPV on the cervix...
June 20, 2018: Epidemiology and Infection
Mona Seyed Attaran, Seyed Masoud Hosseini, Javad Fakhari, Zohreh Sharifi
Background and Objectives: Hepatitis B virus (HBV) is an enveloped DNA virus belongs to Hepadnaviridea family. HBV infection is a serious global health problem, with 2 billion people infected worldwide, and 350 million suffering from chronic HBV infection. The aim of this study was to determine the serological pattern and molecular characterization of HBV diversity in asymptomatic blood donors in Iran alongside with the mutation status of HBV in relation to blood safety. Materials and Methods: One hundred and sixty six samples from asymptomatic blood donors who were positive for hepatitis B surface antigen during 2012 to 2014 were selected...
February 2018: Iranian Journal of Microbiology
Ye Gu, Jing Zhao, Li Ao, Jianning Ma, Kena Bao, Min Liu, Weiping Huang
It is reported that glutathione S-transferase mu (GSTM1) polymorphism is associated with non-viral hepatic cirrhosis (HC). However, some studies showed different views. Therefore, in this paper, a meta-analysis was conducted to get a more comprehensive understanding of GSTM1 polymorphisms in non-viral HC susceptibility. The results showed that GSTM1 null was associated with the increased risk of non-viral HC (OR = 1.337, 95% CI 1.112-1.804, p = 0.005). Subgroup analysis of cirrhosis type revealed that GSTM1 null was a prominent risk factor for alcoholic HC (OR = 1...
June 19, 2018: European Journal of Medical Research
Andreza Lemos Salvio, Amanda de Oliveira Lopes, Adilson José de Almeida, Noemi Rovaris Gardinali, Lyana Ribeiro Pinto Lima, Jaqueline Mendes de Oliveira, Fernando Samuel Sion, Luiz Claudio Pereira Ribeiro, Marcelo Alves Pinto, Vanessa Salete de Paula
AIMS: To improve RT-qPCR with an internal control and a synthetic standard curve to detect HEV in HIV coinfected patients. METHODS AND RESULTS: A single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA) synthetic curve were designed, compared to the international reference panel for HEV genotypes, and tested to quantify and detect a reference panel for HEV genotypes. The detection limit of the RNA synthetic curve (50 copies/mL) was better than the DNA synthetic curve (100 copies/mL) and the WHO standard curve (250 copies/mL)...
June 19, 2018: Journal of Applied Microbiology
Ayesha Zafar, Muhammad Imran, Sarmad Zahoor, Zaheer Hussain Shah, Muhammad Ali, Muhammad Sohail Afzal
In Pakistan, around 11 million people are infected with, at least, six circulating genotypes and subtypes of hepatitis C virus (HCV). The viral burden is still on rise. HCV genotype determination is necessary to devise appropriate antiviral therapy. Previous reports highlight the prevalence circulating untypable (in diagnostic perspectives) HCV subtype in local Pakistani patients. Present study was designed to investigate the epidemiological distribution of genotypes in different districts of Punjab, Pakistan and focuses on prevalence of diagnostically untypable subtype and its possible antiviral therapy...
June 19, 2018: Viral Immunology
Yuji Teraoka, Takuro Uchida, Michio Imamura, Nobuhiko Hiraga, Mitsutaka Osawa, Hiromi Kan, Yuhei Saito, Masataka Tsuge, Hiromi Abe-Chayama, C Nelson Hayes, Grace Naswa Makokha, Hiroshi Aikata, Daiki Miki, Hidenori Ochi, Yuji Ishida, Chise Tateno, Kazuaki Chayama
Combined daclatasvir (DCV)/asunaprevir (ASV) plus beclabuvir (BCV) treatment shows a high virological response for genotype 1b chronic hepatitis C patients. However, its efficacy for patients for whom previous direct-acting antiviral (DAA) therapy failed is not known. We analysed the efficacy of DCV/ASV/BCV treatment for HCV-infected mice and chronic hepatitis patients. Human hepatocyte chimaeric mice were injected with serum samples obtained from either a DAA-naïve patient or a DCV/ASV treatment failure and were then treated with DCV/ASV alone or in combination with BCV for 4 weeks...
June 19, 2018: Journal of General Virology
Megan Sue Soliman, Youssef Yousry Soliman, Qamar Ahmad, Roopa Yarlagadda
Hepatocellular carcinoma (HCC) is the most common primary liver cancer and is the third highest cause of cancer mortality worldwide. Risk factors include chronic liver disease and cirrhosis of various causes including chronic hepatitis B and C. In cases of chronic hepatitis C virus (HCV), HCC usually does not manifest unless the liver has become cirrhotic. Fortunately, novel treatments for hepatitis C including ledipasvir/sofosbuvir can cure patients from their disease and as a result, may never develop cirrhosis and therefore, be at much lower risk of developing HCC...
2018: Journal of Community Hospital Internal Medicine Perspectives
Calvin Q Pan, Benjamin C Tiongson, Ke-Qin Hu, Steven-Huy B Han, Myron Tong, Danny Chu, James Park, Tai Ping Lee, Kalyan Ram Bhamidimarri, Xiaoli Ma, Pei Ying Xiao, Smruti R Mohanty, Dan Wang
BACKGROUND: Limited data exist with regard to treatment outcomes in Asian Americans with chronic hepatitis C (CHC). We evaluated sofosbuvir (SOF)-based regimens in a national cohort of Asian Americans. METHODS: Eligible Asian Americans patients with CHC who had posttreatment follow-up of 24 weeks for SOF -based therapies from December 2013 to June 2017 were enrolled from 11 sites across the United States. The primary endpoint was sustained virologic response (SVR) rates at posttreatment weeks 12 and 24...
June 16, 2018: Journal of Clinical Gastroenterology
Nicole Le-Corre, Sebastián Barría, Tania López, Constanza Martínez-Valdebenito, Ana M Contreras, Marcela Ferrés
Mumps virus usually produces a benign infection characterized by increased parotid volume which, prior to vaccination, mainly affected children and adolescents. After the introduction of measles, mumps and rubella (MMR) vaccine, mumps incidence decreased dramatically. This intervention also produced a change in its clinical presentation, moving to young adult patients, with an increased risk of complications. We report two clinical mumps cases in young adults with different clinical presentations. In both cases, serologic assays were assessed and, in one case, a polymerase chain reaction (PCR) was performed in order to confirm the diagnosis...
April 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Sergio Alejandro Gómez-Ochoa
BACKGROUND: Plasma viremia is a factor whose role in the severity of dengue virus infection has been widely discussed in recent years, with divergent results found in the multiple contexts evaluated. METHODS: A systematic review of the literature using the Scopus, EBSCOhost, SpringerLink, Lilacs and Scielo databases was conducted. For inclusion in the review there were considered studies that evaluated the relationship between plasma viral load and clinical manifestations or severity of the disease...
April 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Tamara Ruegamer, Rebecca Hoffmann, Anette Rohrhofer, Franz Audebert, Bernd Salzberger, Klaus Korn, Philipp Schuster, Jutta Eichler, Barbara Schmidt
OBJECTIVE(S): Up to 40% of HIV-1 infected individuals are coinfected with human pegivirus type 1 (HPgV-1). The majority, but not all studies reported a beneficial effect of HPgV-1 coinfection on HIV-1 disease progression. So far, the impact of different HPgV-1 genotypes (GT) on different HIV-1 subtypes remains unclear. METHODS: Peptides derived from HPgV-1 envelope protein E2, and representing different viral genotypes, were synthesized using Fmoc/t-Bu based solid phase peptide synthesis...
June 14, 2018: AIDS
Asok Kumar Mariappan, Palanivelu Munusamy, Deepak Kumar, Shyma K Latheef, Shambu Dayal Singh, Rajendra Singh, Kuldeep Dhama
Newcastle disease virus (NDV) belongs to genus Avulavirus and family Paramyxoviridae. There are thirteen serotypes named APMV-I (Avian Paramyxovirus-I) to APMV-13 of which NDV has been designated as APMV-1. The disease has been reported worldwide affecting both domestic and wild avian species. Morbidity and mortality rates up to 100% have been reported in cases of unvaccinated flocks. Stringent vaccination schedule is practiced in endemic/disease prone areas in order to prevent the disease. Despite this, NDV outbreaks have been reported even in cases of vaccinated populations...
June 2018: Virusdisease
Supriya Mahajan, Manish Chandra Choudhary, Guresh Kumar, Ekta Gupta
Dried blood spot (DBS) is a minimally invasive sampling method suitable for sample collection, storage and transportation in resource limited areas. Aim of this study was to compare the diagnostic utility of DBS with plasma sample for HCV RNA quantitation and genotyping using commercial systems. Plasma and DBS card spotted samples were collected from 95 HCV seropositive patients. Both types of samples were subjected to HCV RNA by real-time PCR (Abbott m2000rt, USA). Genotyping was performed using Abbott HCV genotype II kit (Abbott diagnostics, USA) in samples with viral load > 3 log10  IU/ml...
June 2018: Virusdisease
Babak Sayad, Yosra Naderi, Seyed Moayed Alavian, Farid Najafi, Alireza Janbakhsh, Feyzollah Mansouri, Siavash Vaziri, Mandana Afsharian, Fatemeh Norooznezhad
Aim: This study aimed to determine the seroprevalence and viremic infection of hepatitis delta virus (HDV) in Kermanshah. Background: Hepatitis delta is one of the most complex viral infections of liver that along with hepatitis B virus could lead to fulminant hepatitis, progressive chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Methods: Referrals with positive HBs Ag were included and tested for HDV Ab using ELISA. Seropositives were subsequently evaluated for viremia by assaying HDV RNA and HBV DNA using real-time PCR...
2018: Gastroenterology and Hepatology From Bed to Bench
Abid Shoukat, Mosin S Khan, Syed Mudassar, Zaffar Kawoosa, Altaf H Shah, Showkat Ali Zargar
Background: In hepatitis C virus (HCV), infection viral and IL28B genotype along with many clinical and biochemical factors can influence response rates to pegylated interferon plus ribavirin (Peg-IFN-a/R) therapy and progression to chronic hepatitis C (CHC). Aims: The present study was conducted to determine the effect of biochemical and risk factors on treatment outcome in CHC patients in relation to their viral and host genotype. Settings and Design: The present study was a prospective Pe- IFN efficacy study consisting of Peg-IFN-a/R therapy for 24-48 weeks including 250 HCV infected patients...
April 2018: Journal of Global Infectious Diseases
Charlotta Millbourn, Afrodite Psaros Einberg, Gudrun Lindh, Ingegerd Hökeberg, Björn Fischler, Karin Lindahl
OBJECTIVES: The prevalence of hepatitis C virus (HCV) infection in Sweden is estimated to 0.5%. Before 1992, blood transfusion posed a risk of HCV transmission. The primary aim of this study was to estimate anti-HCV prevalence in Stockholm County among individuals receiving blood transfusions 1965-1991. The secondary aim was to study the effect of age at transfusion on the development of liver disease and treatment outcome. MATERIALS AND METHODS: This is a retrospective analysis of individuals found to be anti-HCV tested positive in Stockholm County during a national screening campaign in Sweden 2008-2010...
June 18, 2018: Scandinavian Journal of Gastroenterology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"