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https://www.readbyqxmd.com/read/28724048/impact-of-alcohol-consumption-among-patients-in-hepatitis-c-virus-treatment
#1
Andréa Carvalho Maia Vieira-Castro, Luiz Carlos Marques de Oliveira
BACKGROUND: Recent studies have questioned the recommendation of abstinence from alcohol for at least 6 months for alcoholic patients to be treated for hepatitis C. OBJECTIVE: The present study aimed to assess the impact of alcohol consumption among patients undergoing hepatitis C treatment. METHODS: In this cross-sectional study, 121 patients [78 (64.5%) men; 28-70 years] were evaluated. They were divided as follows: patients who consumed <12 g of ethanol/day throughout life (Group 1), 12-59 g/day (Group 2) and ≥60 g/day (Group 3)...
July 13, 2017: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/28722331/hepatitis-c-virus-and-proprotein-convertase-subtilisin-kexin-type-9-a-detrimental-interaction-to-increase-viral-infectivity-and-disrupt-lipid-metabolism
#2
REVIEW
Matteo Pirro, Vanessa Bianconi, Daniela Francisci, Elisabetta Schiaroli, Francesco Bagaglia, Amirhossein Sahebkar, Franco Baldelli
From viral binding to the hepatocyte surface to extracellular virion release, the replication cycle of the hepatitis C virus (HCV) intersects at various levels with lipid metabolism; this leads to a derangement of the lipid profile and to increased viral infectivity. Accumulating evidence supports the crucial regulatory role of proprotein convertase subtilisin/kexin type 9 (PCSK9) in lipoprotein metabolism. Notably, a complex interaction between HCV and PCSK9 has been documented. Indeed, either increased or reduced circulating PCSK9 levels have been observed in HCV patients; this discrepancy might be related to several confounders, including HCV genotype, human immunodeficiency virus (HIV) coinfection and the ambiguous HCV-mediated influence on PCSK9 transcription factors...
July 18, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28720147/safety-and-immunogenicity-of-the-quadrivalent-human-papillomavirus-qhpv-vaccine-in-hiv-positive-spanish-men-who-have-sex-with-men-msm
#3
Carmen Hidalgo-Tenorio, Jessica Ramírez-Taboada, Concepción Gil-Anguita, Javier Esquivias, Mohamed Omar-Mohamed-Balgahata, Antonio SamPedro, Miguel Lopez-Ruz, Juan Pasquau
BACKGROUND: Safety and immunogenicity of the quadrivalent human papillomavirus (qHPV) vaccine were evaluated in HIV-positive Spanish MSM. The prevalence of High Squamous Intraepithelial Lesions (HSIL) and genotypes of high-risk human papillomavirus (HR-HPV) were also determined, as well as risk factors associated with the presence of HR-HPV in anal mucosa. METHODS: This is a randomised, double blind, placebo-controlled trial of the quadrivalent HPV (qHPV) vaccine...
July 18, 2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28719029/directly-observed-therapy-of-sofosbuvir-ribavirin-peginterferon-with-minimal-monitoring-for-the-treatment-of-chronic-hepatitis-c-in-people-with-a-history-of-drug-use-in-chennai-india-c-dot
#4
Sunil S Solomon, Mark S Sulkowski, Pradeep Amrose, Aylur K Srikrishnan, Allison M McFall, Balakrishnan Ramasamy, Muniratnam S Kumar, Santhanam Anand, David L Thomas, Shruti H Mehta
BACKGROUND AND AIMS: We assessed the feasibility of field-based directly observed therapy (DOT) with minimal monitoring to deliver HCV treatment to people with a history of drug use in Chennai, India. METHODS: 50 participants were randomized 1:1 to sofosbuvir + peginterferon alfa 2a + ribavirin (SOF+PR) for 12 weeks (Arm 1) vs. sofosbuvir + ribavirin (SOF+R) for 24 weeks (Arm 2). SOF+R was delivered daily at participant chosen venues and weekly peginterferon injections at the study clinic...
July 18, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28718943/hepatitis-e-virus-orf-1-induces-proliferative-and-functional-t-cell-responses-in-patients-with-ongoing-and-resolved-hepatitis-e
#5
Jana Al-Ayoubi, Patrick Behrendt, Birgit Bremer, Pothakamuri Venkata Suneetha, Anett Gisa, Franziska Rinker, Michael P Manns, Markus Cornberg, Heiner Wedemeyer, Anke R M Kraft
BACKGROUND AND AIMS: Hepatitis E virus (HEV) is a major cause of acute viral hepatitis with > 3 million symptomatic cases per year accounting for 70,000 HEV-related deaths. HEV-specific T-cell responses have been investigated against structural proteins expressed by open reading frames (ORF) 2 and 3. T-cell responses against non-structural HEV proteins encoded by ORF1 are hardly studied. The aim of this study was to determine HEV ORF1-specific T-cell responses in comparison to ORF2/3 in patients exposed to HEV...
July 18, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28717857/concordance-of-hpv-load-and-hpv-mrna-for-16-carcinogenic-possibly-carcinogenic-hpv-types-in-paired-smear-tissue-cervical-cancer-specimens
#6
Gordana Halec, Bolormaa Dondog, Michael Pawlita, Markus Schmitt
HPV types with high viral load are associated with cervical abnormalities. However, viral load measurements and concordance of HPV loads and viral mRNA have not been demonstrated for all high-risk/possibly high-risk (HR-/pHR-)HPV types in cervical cancer (CxCa). Especially, the biological role of co-infecting HR-/pHR-HPV types with low viral load has not been thoroughly investigated. Using BSGP5+/6+-PCR/MPG genotyping, we analyzed viral loads for all currently defined 51 mucosal HPV types in 74 cervical smears from patients with CxCa and compared this data with HPV DNA and mRNA status in these patients' corresponding CxCa tissues...
July 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28717342/prevalence-of-high-risk-genotypes-of-human-papillomavirus-women-diagnosed-with-premalignant-and-malignant-pap-smear-tests-in-southern-ecuador
#7
Paola Dalgo Aguilar, Cisne Loján González, Ana Córdova Rodríguez, Katherine Acurio Páez, Ana Paulina Arévalo, Jana Bobokova
Human papillomavirus (HPV) is the primary infectious agent for the development of cervical cancer, although the presence of the virus alone is insufficient for viral development and proliferation; this can be attributed to the increase in potential oncogenic risk, along with other risk factors. In the present investigation, the prevalence of high-risk HPV was determined from samples of premalignant or malignant cervical cytology in women from the southern region of Ecuador. The kit we used was able to detect genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59...
2017: Infectious Diseases in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28717061/apobec-mediated-genomic-alterations-link-immunity-and-viral-infection-during-human-papillomavirus-driven-cervical-carcinogenesis
#8
Lanting Chen, Xuemin Qiu, Na Zhang, Yan Wang, Mingyan Wang, Dajin Li, Ling Wang, Yan Du
Cervical cancer is one of the most frequently diagnosed cancers and is a major cause of death from gynecologic cancers worldwide; the cancer burden from cervical cancer is especially heavy in less developed countries. Most cases of cervical cancer are caused by persistent infection with carcinogenic human papillomavirus (HPV) genotypes 16 and 18. Non-resolving inflammation caused by HPV infection provides a microenvironment that facilitates cancer development. Molecular alterations during the process of HPV-induced carcinogenesis are characterized by DNA methylation within the HPV genome, promoter hypermethylation of tumor suppressor genes in the host genome, as well as genomic instability caused by viral DNA integrating into the host genome...
July 17, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28715422/unraveling-the-genetic-diversity-and-phylogeny-of-leishmania-rna-virus-1-strains-of-infected-leishmania-isolates-circulating-in-french-guiana
#9
Sourakhata Tirera, Marine Ginouves, Damien Donato, Ignacio S Caballero, Christiane Bouchier, Anne Lavergne, Eliane Bourreau, Emilie Mosnier, Vincent Vantilcke, Pierre Couppié, Ghislaine Prevot, Vincent Lacoste
INTRODUCTION: Leishmania RNA virus type 1 (LRV1) is an endosymbiont of some Leishmania (Vianna) species in South America. Presence of LRV1 in parasites exacerbates disease severity in animal models and humans, related to a disproportioned innate immune response, and is correlated with drug treatment failures in humans. Although the virus was identified decades ago, its genomic diversity has been overlooked until now. METHODOLOGY/PRINCIPLES FINDINGS: We subjected LRV1 strains from 19 L...
July 17, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28713830/the-prevalence-and-replication-capacity-of-a-tibetan-dominant-hbv-strain-c-d-recombinant
#10
Lingyao Du, Menghan Liu, Miao Liu, Taoyou Zhou, Xing Cheng, Cong Liu, Hong Tang
This study aimed to estimate the distribution of hepatitis B virus (HBV) C/D recombinant in Han and Tibet patients with chronic hepatitis B (CHB) and then learn such strain's replication capacity in vivo. A total of 331 serum samples were collected from Han outpatients from Sichuan Province and Tibetan outpatients from Tibet. Viral genotypes in these samples were identified. An HBV replicative plasmid of C/D recombinant was constructed with selected genome. Sequentially, HBV replicative mouse models were established and the replication capacity of the viral strain was studied in vivo...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28713579/ring-trial-2016-for-bluetongue-virus-detection-by-real-time-rt-pcr-in-france
#11
Corinne Sailleau, Cyril Viarouge, Emmanuel Breard, Damien Vitour, Stephan Zientara
Since the unexpected emergence of BTV-8 in Northern Europe and the incursion of BTV-8 and 1 in France in 2006-2007, molecular diagnosis has considerably evolved. Several real-time RT-PCR (rtRT-PCR) methods have been developed and published, and are currently being used in many countries across Europe for BTV detection and typing. In France, the national reference laboratory (NRL) for orbiviruses develops and validates 'ready-to-use' kits with private companies for viral RNA detection. The regional laboratories network that was set up to deal with a heavy demand for analyses has used these available kits...
May 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/28710798/can-quantitative-hepatitis-b-surface-antigen-levels-predict-the-severity-of-liver-disease-in-genotype-e-patients
#12
Gayatri Chakrabarty, Matthew Bruce, Mary Horner, Bo Wang, Kosh Agarwal, Ivana Carey
The utility of quantitative Hepatitis B surface antigen (qHBsAg) level as a marker of chronic hepatitis B (CHB)-related liver damage is not fully delineated, but is becoming increasingly relevant. Quantitative HBsAg levels are linked with progression of liver disease in HBeAg-negative genotype B and C patients, but it is not clear whether this is consistent across all HBV genotypes. In this single-center, cross-sectional observational study, we evaluated whether qHBsAg levels can predict the severity of liver disease in genotype E patients...
July 14, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28710536/resistance-to-tomato-leaf-curl-new-delhi-virus-in-melon-is-controlled-by-a-major-qtl-located-in-chromosome-11
#13
Cristina Sáez, Cristina Esteras, Cecilia Martínez, María Ferriol, Narinder P S Dhillon, Carmelo López, Belén Picó
Identification of three genomic regions and underlying candidate genes controlling the high level of resistance to ToLCNDV derived from a wild melon. SNP markers appropriated for MAS management of resistance. Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus that severely affects melon crop (Cucumis melo) in the main production areas of Spain since 2012. In this work, we evaluated the degree of resistance of four accessions (two belonging to the subsp. agrestis var. momordica and two to the wild agrestis group) and their corresponding hybrids with a susceptible commercial melon belonging to the subsp...
July 14, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28708973/concordance-of-beta-papillomavirus-across-anogenital-and-oral-anatomic-sites-of-men-the-him-study
#14
Emily M Nunes, Rossana V M López, Staci L Sudenga, Tarik Gheit, Massimo Tommasino, Maria L Baggio, Silvaneide Ferreira, Lenice Galan, Roberto C Silva, Eduardo Lazcano-Ponce, Anna R Giuliano, Luisa L Villa, Laura Sichero
We evaluated the concordance between β-HPVs detected in external genital skin, anal canal, and oral cavity specimens collected simultaneously from 717 men that were participating in the multinational HIM Study. Viral genotyping was performed using the Luminex technology. Species- and type-specific concordance was measured using kappa statistics for agreement. Overall, concordance of β-HPVs across sites was low and mainly observed among paired genital/anal canal samples. When grouped by species, solely β-4 HPVs showed moderate concordance in genital/anal pairs (κ = 0...
July 11, 2017: Virology
https://www.readbyqxmd.com/read/28707600/a-new-potent-ns5a-inhibitor-in-the-management-of-hepatitis-c-virus-ravidasvir
#15
Enas Hafez, Tamer Elbaz, Mohamed El Kassas, Gamal Esmat
Chronic hepatitis C virus (HCV) is a worldwide health problem that can lead to liver cirrhosis, liver cell failure and numerous subsequent complications such as hepatocellular carcinoma. Till the near past, pegylated interferon was the standard of care therapy. However, it was associated with suboptimal success rates and many side effects. Thereafter, direct antiviral agents (DAA) appeared and played the key role in management of HCV. One of those recent DAA is ravidasvir. It is a potent NS5A inhibitor that was formerly known as PPI-668...
July 13, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/28706414/risk-of-hepatitis-b-reactivation-in-patients-treated-with-direct-acting-antivirals-for-hepatitis-c
#16
EDITORIAL
Ioanna Aggeletopoulou, Christos Konstantakis, Spilios Manolakopoulos, Christos Triantos
The recent introduction of direct-acting antiviral drugs (DAAs) for treatment of the hepatitis C virus (HCV) has greatly improved the management of HCV for infected patients. These viral protein inhibitors act rapidly, allowing HCV clearance and increasing the sustained virological response rates. However, hepatitis B virus (HBV) reactivation has been reported in HCV/HBV co-infected patients. Hepatitis B reactivation refers to an abrupt increase in the HBV and is well-documented in patients with previously undetected HBV DNA due to inactive or resolved HBV infection...
June 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28705717/whole-genome-characterization-of-uruguayan-strains-of-avian-infectious-bronchitis-virus-reveals-extensive-recombination-between-the-two-major-south-american-lineages
#17
Ana Marandino, Gonzalo Tomás, Yanina Panzera, Gonzalo Greif, Adriana Parodi-Talice, Martín Hernández, Claudia Techera, Diego Hernández, Ruben Pérez
Infectious bronchitis virus (Gammacoronavirus, Coronaviridae) is a genetically variable RNA virus that causes one of the most persistent respiratory diseases in poultry. The virus is classified in genotypes and lineages with different epidemiological relevance. Two lineages of the GI genotype (11 and 16) have been widely circulating for decades in South America. GI-11 is an exclusive South American lineage while the GI-16 lineage is distributed in Asia, Europe and South America. Here, we obtained the whole genome of two Uruguayan strains of the GI-11 and GI-16 lineages using Illumina high-throughput sequencing...
July 10, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28705178/hepatitis-a-virus-genotypes-and-strains-from-an-endemic-area-of-europe-bulgaria-2012-2014
#18
Roberto Bruni, Stefania Taffon, Michele Equestre, Eleonora Cella, Alessandra Lo Presti, Angela Costantino, Paola Chionne, Elisabetta Madonna, Elitsa Golkocheva-Markova, Diljana Bankova, Massimo Ciccozzi, Pavel Teoharov, Anna Rita Ciccaglione
BACKGROUND: Hepatitis A virus (HAV) infection is endemic in Eastern European and Balkan region countries. In 2012, Bulgaria showed the highest rate (67.13 cases per 100,000) in Europe. Nevertheless, HAV genotypes and strains circulating in this country have never been described. The present study reports the molecular characterization of HAV from 105 patients from Bulgaria. METHODS: Anti-HAV IgM positive serum samples collected in 2012-2014 from different towns and villages in Bulgaria were analysed by nested RT-PCR, sequencing of the VP1/2A region and phylogenetic analysis; the results were analysed together with patient and geographical data...
July 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28702675/tnf-%C3%AE-genetic-predisposition-and-higher-expression-of-inflammatory-pathway-components-in-keratoconus
#19
Muneeza Arbab, Saira Tahir, Muhammad Khizar Niazi, Mazhar Ishaq, Alamdar Hussain, Pir Muhammad Siddique, Sadia Saeed, Wajid Ali Khan, Raheel Qamar, Azeem Mehmood Butt, Maleeha Azam
Purpose: To date keratoconus (KC) pathogenesis is undefined; however, the involvement of inflammatory pathways in disease development is becoming apparent. In the present study, we investigated the role of a promoter region polymorphism rs1800629 (-308G>A) in the inflammatory pathway component TNF-α and its effects on the expression of TNF-α and downstream molecules tumor necrosis factor receptor 1 and 2 (TNFR1 and TNFR2), v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), and interleukin 6 (IL-6) in KC development...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28701405/propagation-of-astrovirus-va1-a-neurotropic-human-astrovirus-in-cell-culture
#20
Andrew B Janowski, Irma K Bauer, Lori R Holtz, David Wang
Astrovirus VA1/HMO-C (VA1; mamastrovirus 9) is a recently discovered astrovirus genotype that is divergent from the classic human astroviruses (mamastrovirus 1). The gastrointestinal tract is presumed to be the primary site of infection and pathogenicity for astroviruses. However, VA1 has been independently detected in brain tissue of five cases of human encephalitis. Studies of the pathogenicity of VA1 are currently impossible because there are no reported cell culture systems or in vivo models that support VA1 infection...
July 12, 2017: Journal of Virology
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