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https://www.readbyqxmd.com/read/29682246/distribution-of-hepatitis-c-virus-genotypes-in-patients-with-major-%C3%AE-thalassemia-in-mashhad-northeast-iran
#1
Sanaz Ahmadi-Ghezeldasht, Zahra Badiei, Hamid Reza Sima, Mohammad Reza Hedayati-Moghaddam, Meysam Habibi, Mohsen Khamooshi, Ahmad Azimi
BACKGROUND Hepatitis C virus (HCV) is considered to be the major cause of post-transfusion hepatitis in patients with thalassemia. We aimed to determine the HCV prevalence, genotypes, and viral load among patients with major β-thalassemia in Mashhad, Iran. METHODS Medical records of all 550 patients with major β-thalassemia who referred to ThalassemiaHemophilia Center of Mashhad (Sarvar Clinic) were reviewed from October to November 2011. Plasma samples of the patients were tested for the presence of anti-HCV antibodies by enzyme linked immunosorbent assay...
January 2018: Middle East Journal of Digestive Diseases
https://www.readbyqxmd.com/read/29680507/phylodynamics-of-merkel-cell-polyomavirus-and-human-polyomavirus-6-a-long-term-history-with-humans
#2
Carolina Torres, Melina Elizabeth Barrios, Robertina Viviana Cammarata, Matías Victoria, Xavier Fernandez-Cassi, Silvia Bofill-Mas, Rodney Colina, María Dolores Blanco Fernández, Viviana Andrea Mbayed
New human polyomaviruses have been described in the last years, including the Merkel-cell polyomavirus (MCPyV; Human polyomavirus 5) and the Human polyomavirus 6 (HPyV6). Although their infection is usually asymptomatic, in immunocompromised host can cause life-threatening pathologies, such as the Merkel cell carcinoma, an aggressive skin neoplasia associated to the MCPyV. Despite being prevalent viruses in population, epidemiological data from South America are scarce, as well as the characterization of the viral types circulating and their origin...
April 19, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29676846/generic-sofosbuvir-based-direct-acting-antivirals-in-hepatitis-c-virus-infected-patients-with-chronic-kidney-disease
#3
Manoj Kumar Sharma, Suman Lata Nayak, Ekta Gupta, Ashish Kataria, Shiv K Sarin
BACKGROUND & AIMS: There is scant data on use of sofosbuvir containing directly acting anti-viral (DAA) regimens in chronic kidney disease (CKD) patients. Recently generic versions of DAAs have become available in low income countries including India. The aim of this study was to study the efficacy and safety of generic sofosbuvir in combination with generic ribavirin, ledipasvir, or daclatasvir in HCV infected patients with CKD including patients with advanced CKD (CKD stage 4 or 5 with an estimated glomerular filtration rate (GFR) <30 mL/min or those on dialysis)...
April 20, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29675993/effects-of-masp2-haplotypes-and-masp-2-levels-in-hepatitis-c-infected-patients
#4
Amanda A Silva, Sandra J Catarino, Angelica B W Boldt, Maria Lucia A Pedroso, Marcia H Beltrame, Iara J Messias-Reason
Mannan-binding lectin (MBL) and MBL-associated serine protease 2 (MASP-2) are components of the lectin pathway, which activate the complement system after binding to the HCV structural proteins E1 and E2. We haplotyped 11 MASP2 polymorphisms in 103 HCV patients and 205 controls and measured MASP-2 levels in 67 HCV patients and 77 controls to better understand the role of MASP-2 in hepatitis C susceptibility and disease severity according to viral genotype and fibrosis levels. The haplotype block MASP2*ARDP was associated with protection against HCV infection (OR = 0...
April 19, 2018: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/29675889/acute-liver-failure-caused-by-hepatitis-e-virus-genotype-3-and-4-a-systematic-review-and-pooled-analysis
#5
Samir Haffar, Shalimar, Ravinder Jeet Kaur, Zhen Wang, Larry Prokop, M Hassan Murad, Fateh Bazerbachi
BACKGROUND: Acute liver failure (ALF) caused by hepatitis E virus (HEV) genotype 3 and 4 has been rarely described. Due to the presence of a short golden therapeutic window in patients with viral ALF from other causes, it is possible that early recognition and treatment might reduce the morbidity and mortality. We performed a systematicac review and pooled analysis of ALF caused by HEV genotype 3 and 4. METHODS: Two reviewers appraised studies after searching multiple databases on June 12th , 2017...
April 19, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29674053/chimeric-dna-lna-based-biosensor-for-the-rapid-detection-of-african-swine-fever-virus
#6
Massimo Biagetti, Massimiliano Cuccioloni, Laura Bonfili, Valentina Cecarini, Carla Sebastiani, Ludovica Curcio, Monica Giammarioli, Gian Mario De Mia, Anna Maria Eleuteri, Mauro Angeletti
African swine fever (ASF) virus is a DNA virus responsible for a severe haemorrhagic fever in pigs, which (still in the absence of vaccination strategies) results in high mortality rates. Herein, we present a biosensor-based method for the detection of ASF viral DNA in the blood of pigs. The biosensor exploits a single-strand DNA probe with locked nucleic acid nucleotides (LNA) substitutions as the complementary recognition element for the conserved region of vp72 gene of ASF virus. The biosensor was calibrated using qPCR-quantified ASF viral DNA extracted from the blood of pigs experimentally infected with the virulent Italian isolate 49/08, genotype I...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29670609/enhanced-replication-of-virulent-newcastle-disease-virus-in-chicken-macrophages-is-due-to-polarized-activation-of-cells-by-inhibition-of-tlr7
#7
Pingze Zhang, Zhuang Ding, Xinxin Liu, Yanyu Chen, Junjiao Li, Zhi Tao, Yidong Fei, Cong Xue, Jing Qian, Xueli Wang, Qingmei Li, Tobias Stoeger, Jianjun Chen, Yuhai Bi, Renfu Yin
Newcastle disease (ND), caused by infections with virulent strains of Newcastle disease virus (NDV), is one of the most important infectious disease affecting wild, peridomestic, and domestic birds worldwide. Vaccines constructed from live, low-virulence (lentogenic) viruses are the most accepted prevention and control strategies for combating ND in poultry across the globe. Avian macrophages are one of the first cell lines of defense against microbial infection, responding to signals in the microenvironment...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29670059/variability-studies-of-two-prunus-infecting-fabaviruses-with-the-aid-of-high-throughput-sequencing
#8
Igor Koloniuk, Tatiana Sarkisova, Karel Petrzik, Ondřej Lenz, Jaroslava Přibylová, Jana Fránová, Josef Špak, Leonidas Lotos, Christina Beta, Asimina Katsiani, Thierry Candresse, Varvara I Maliogka
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF). Both viruses are most homologous to members of the Fabavirus genus ( Secoviridae family)...
April 18, 2018: Viruses
https://www.readbyqxmd.com/read/29670022/whole-genome-classification-and-phylogenetic-analyses-of-rotavirus-b-strains-from-the-united-states
#9
Frances K Shepherd, Diana Maria Herrera-Ibata, Elizabeth Porter, Nitipong Homwong, Richard Hesse, Jianfa Bai, Douglas G Marthaler
Rotaviruses (RVs) are a major etiological agent of acute viral gastroenteritis in humans and young animals, with rotavirus B (RVB) often detected in suckling and weaned pigs. Group A rotavirus classification is currently based on the two outer capsid proteins, VP7 and VP4, and the middle layer protein, VP6. Using RVB strains generated in this study and reference sequences from GenBank, pairwise identity frequency graphs and phylogenetic trees were constructed for the eleven gene segments of RVB to estimate the nucleotide identity cutoff values for different genotypes and determine the genotype diversity per gene segment...
April 18, 2018: Pathogens
https://www.readbyqxmd.com/read/29669835/characterization-of-three-novel-linear-neutralizing-b-cell-epitopes-in-the-capsid-protein-of-swine-hepatitis-e-virus
#10
Yiyang Chen, Baoyuan Liu, Yani Sun, Huixia Li, Taofeng Du, Yuchen Nan, Julian A Hiscox, En-Min Zhou, Qin Zhao
Hepatitis E virus (HEV) causes liver disease in humans and is thought to be a zoonotic infection with domestic animals being a reservoir including swine and rabbits. One of the proteins encoded by the virus is the capsid protein. This is likely the major immune-dominant protein and a target for vaccination. Four monoclonal antibodies (MAbs); three novel; 1E4, 2C7, 2G9, and one previously characterized (1B5), were evaluated for binding to the capsid protein from genotype 4 (swine) hepatitis E virus (HEV). The results indicated that 625 DFCP628 , 458 PSRPF462 , and 407 EPTV410 peptides on the capsid protein comprised minimal amino acid sequence motifs recognized by 1E4, 2C7, and 2G9, respectively...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29667338/variation-in-the-tek-gene-is-not-associated-with-asthma-but-with-allergic-conjunctivitis
#11
L E Fodor, A Gézsi, Z Gál, A Nagy, A Kiss, A Bikov, C Szalai
The Tie2 receptor is an important player in angiogenesis. The Tie2 mRNA and protein are abundantly expressed in the lungs and the associated pathway also has an important role in the development and function of the eye. Tie2 is encoded by the TEK gene in humans. Recently, variations in the TEK gene have been found associated with asthma. The objective of the present study was to investigate whether variations in the TEK gene influenced the susceptibility to pediatric asthma and/or associated phenotypes like GINA status, viral- or exercise-induced asthma, allergic asthma, indoor, outdoor, inhalative allergies, IgE and eosonophil levels, allergic rhinitis and allergic conjunctivitis...
April 18, 2018: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/29666658/phylogenetic-analysis-of-dengue-virus-in-bangkalan-madura-island-east-java-province-indonesia
#12
Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29664710/analysis-of-host-and-viral-related-factors-associated-to-direct-acting-antiviral-response-in-hepatitis-c-virus-patients
#13
Hafsa Aziz, Muneeba Aziz, Muzaffar Lateef Gill
Hepatitis C virus (HCV) signifies an important health issue as it is a globally prevalent pathogen and poses a great threat to human health. Direct antiviral therapy became a landmark in treatment against chronic HCV infection as they have proven to increase sustained virological response (SVR) rate, provide shortened and simplified regimens. This study aimed to evaluate efficacy of Sofosbuvir and classify factors of treatment success and their function in therapy continuation decision. We studied host and viral factors in 310 patients who received Sofosbuvir (DAA) at a dose of 400 mg daily along with ribavirin 10 mg/kg body weight for 24 weeks and followed by 12 weeks after completion of treatment...
April 2018: Viral Immunology
https://www.readbyqxmd.com/read/29663448/seroprevalence-molecular-epidemiology-and-quantitation-of-parvovirus-b19-dna-levels-in-iranian-blood-donors
#14
Maryam Zadsar, Arezoo Aghakhani, Mohammad Banifazl, Monireh Kazemimanesh, Seyed Morteza Tabatabaei Yazdi, Setareh Mamishi, Anahita Bavand, Mona Sadat Larijani, Amitis Ramezani
Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology and quantitation of B19 DNA levels in blood donors in Tehran, Iran. 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA...
April 16, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29663115/sofosbuvir-plus-daclatasvir-with-or-without-ribavirin-for-treatment-of-chronic-hcv-genotype-4-patients-real-life-experience
#15
G Shiha, R Soliman, M ElBasiony, A A Hassan, N N H Mikhail
INTRODUCTION: New regimens involving direct-acting antiviral agents have recently been approved for the treatment of HCV. Our aim was to assess the efficacy and safety of 12 or 24 weeks of Sofosbuvir 400 mg plus Daclatasvir 60 mg, with or without ribavirin (800-1000 mg) in treating chronic hepatitis C genotype 4 patients. METHODS: This is an open-label observational study that describes the effect of 12 week or 24 weeks of daily oral Sofosbuvir (SOF) 400 mg plus Daclatasvir (DCV) 60 mg with or without ribavirin (RBV) with dose adjustment if indicated...
April 16, 2018: Hepatology International
https://www.readbyqxmd.com/read/29662289/hepatitis-b-virus-pre-s-s-variants-in-liver-diseases
#16
REVIEW
Bing-Fang Chen
Chronic hepatitis B is a global health problem. The clinical outcomes of chronic hepatitis B infection include asymptomatic carrier state, chronic hepatitis (CH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). Because of the spontaneous error rate inherent to viral reverse transcriptase, the hepatitis B virus (HBV) genome evolves during the course of infection under the antiviral pressure of host immunity. The clinical significance of pre-S/S variants has become increasingly recognized in patients with chronic HBV infection...
April 14, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29661652/next-generation-sequencing-reveals-a-high-frequency-of-cxcr4-utilizing-viruses-in-hiv-1-chronically-infected-drug-experienced-individuals-in-south-africa
#17
Nontokozo D Matume, Denis M Tebit, Laurie R Gray, Marie-Louise Hammarskjold, David Rekosh, Pascal O Bessong
BACKGROUND: Entry inhibitors, such as Maraviroc, bind to CCR5 inhibiting entry of CCR5 utilizing viruses (R5 viruses). In the course of HIV infection, CXCR4 utilizing viruses (X4 viruses) may emerge and outgrow R5 viruses, and potentially limit the effectiveness of Maraviroc. The use of Maraviroc is reserved for salvage therapy in South Africa. OBJECTIVE: In this study, we examined the frequency of R5 and X4 viruses, using next generation sequencing, in patients under treatment to draw inferences on the utility of Maraviroc in a South African population...
February 15, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29661434/daclatasvir-and-sofosbuvir-with-or-without-ribavirin-in-liver-transplant-recipients-a-single-center-real-world-study
#18
M Mucenic, A Bandeira de Mello Brandao, C A Marroni, A Medeiros Fleck, M L Zanotelli, G Kiss, M H Meine, I Leipnitz, E Soares Schlindwein, J Martini, A M Costabeber, F K F Sacco, G P Cracco Cantisani
BACKGROUND: Treatment with direct-acting antiviral drugs in interferon-free regimens is currently recommended for viral hepatitis C recurrence after liver transplantation. There are limited data regarding its results in this population, and no optimal treatment scheme has yet been singled out. METHODS: We report our real-world results in liver transplant (LT) recipients. All patients were hepatitis C virus (HCV) monoinfected and completed a 12-week treatment course, followed 12 weeks later by HCV polymerase chain reaction testing with 12 IU/mL sensibility...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29661427/hepatitis-c-virus-eradication-in-kidney-transplant-recipients-a-single-center-experience-in-portugal
#19
A Weigert, S Querido, L Carvalho, L Lebre, C Chagas, P Matias, R Birne, C Nascimento, C Jorge, T Adragão, M Bruges, D Machado
INTRODUCTION: Hepatitis C (HCV) is a major cause of liver impairment post-kidney transplantation (KT). Anti-HCV direct-acting antivirals (DAA) made viral eradication possible. METHODS: We performed a retrospective review of KT patients (n = 23) who received DAA at our hospital. Sustained viral response (SVR) was defined as absence of viral detection 12 weeks after cessation of therapy. RESULTS: From 1985 to September 2017, 1440 patients underwent transplantation at Hospital Santa Cruz...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29658309/individuals-with-hiv-1-subtype-c-infection-and-cryptococcal-meningitis-exhibit-viral-genetic-intermixing-of-hiv-1-between-plasma-and-cerebrospinal-fluid-and-a-high-prevalence-of-cxcr4-using-variants
#20
Katlego Sojane, Richard T Kangethe, Christina C Chang, Mahomed-Yunus Moosa, Sharon Ruth Lewin, Martyn A French, Thumbi Ndung'u
The genotypic properties of HIV-1 subtype C in individuals presenting with cryptococcal meningitis (CM) are not well established. Employing single-genome amplification as well as bulk PCR, cloning and sequencing strategies, we evaluated the genetic properties of HIV-1 subtype C env in 16 antiretroviral therapy naïve study participants with CM. Eleven of the 16 participants had matched blood plasma and cerebrospinal fluid (CSF) evaluated, with the rest having either a plasma or CSF sample evaluated. Before antiretroviral therapy initiation, matched plasma and cerebrospinal fluid-derived env sequences of all 11 participants displayed genetic intermixing between the two compartments...
April 15, 2018: AIDS Research and Human Retroviruses
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