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https://www.readbyqxmd.com/read/28934663/using-a-fluorescence-quenching-method-to-detect-dna-adsorption-onto-single-walled-carbon-nanotube-surfaces
#1
Kazuo Umemura, Shizuma Sato, Gilbert Bustamante, Jing Yong Ye
Surface modification of single-walled carbon nanotubes (SWNTs) with DNA molecules has attracted much attention in recent years to increase SWNT solubility and make various SWNT-based nanobiodevices. Therefore, there is a critical need to quantify the interaction between DNA molecules and SWNT surfaces, particularly the intermediate structures during DNA adsorption. In this study, we demonstrate the ability to detect the adsorption of DNA oligomers on SWNT surfaces by fluorescence spectroscopy. Fluorescein-labelled, 30mer, thymine oligonucleotides (F-T30) were employed as a fluorescent probe to study the interaction of DNA with SWNTs...
September 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28932678/precise-protein-photolithography-p-3-high-performance-biopatterning-using-silk-fibroin-light-chain-as-the-resist
#2
Wanpeng Liu, Zhitao Zhou, Shaoqing Zhang, Zhifeng Shi, Justin Tabarini, Woonsoo Lee, Yeshun Zhang, S N Gilbert Corder, Xinxin Li, Fei Dong, Liang Cheng, Mengkun Liu, David L Kaplan, Fiorenzo G Omenetto, Guozheng Zhang, Ying Mao, Tiger H Tao
Precise patterning of biomaterials has widespread applications, including drug release, degradable implants, tissue engineering, and regenerative medicine. Patterning of protein-based microstructures using UV-photolithography has been demonstrated using protein as the resist material. The Achilles heel of existing protein-based biophotoresists is the inevitable wide molecular weight distribution during the protein extraction/regeneration process, hindering their practical uses in the semiconductor industry where reliability and repeatability are paramount...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28932397/undiagnosed-and-diagnosed-diabetes-mellitus-among-hospitalised-acute-heart-failure-patients-in-botswana
#3
Julius Chacha Mwita, Mgaywa Gilbert Mjungu Damas Magafu, Bernard Omech, Billy Tsima, Matthew J Dewhurst, Monkgogi Goepamang, Yohana Mashalla
OBJECTIVE: The objective of this study was to determine the burden of diagnosed and undiagnosed type 2 diabetes mellitus among patients hospitalised with acute heart failure in Botswana. METHODS: The study enrolled 193 consecutive patients admitted with acute heart failure to the medical wards at Princess Marina Hospital in Gaborone. Patients were classified as previously known diabetics, undiagnosed diabetics (glycated haemoglobin ≥ 6.5%) or as non-diabetics (glycated haemoglobin < 6...
2017: SAGE Open Medicine
https://www.readbyqxmd.com/read/28931520/dna-priming-increases-frequency-of-t-cell-responses-to-a-vsv-hiv-vaccine-with-specific-enhancement-of-cd8-t-cell-responses-by-il-12-pdna
#4
Shuying S Li, Nidhi K Kochar, Marnie Elizaga, Christine M Hay, Gregory J Wilson, Kristen W Cohen, Stephen C De Rosa, Rong Xu, Ayuko Ota-Setlik, Daryl Morris, Greg Finak, Mary Allen, Hong-Van Tieu, Ian Frank, Magdalena E Sobieszczyk, Drew Hannaman, Raphael Gottardo, Peter B Gilbert, Georgia D Tomaras, Lawrence Corey, David K Clarke, Michael A Egan, John H Eldridge, M Juliana McElrath, Nicole Frahm
HVTN 087 assessed the effect of increasing doses of pIL-12 adjuvant on the immunogenicity of a HIV-1 multi-antigen (MAG) DNA vaccine delivered by electroporation and boosted with an attenuated VSV-Gag vaccine. We randomized 100 healthy adults to receive placebo, or 3mg HIV-MAG (gag/pol, env, nef/tat/vif) DNA vaccine co-administered with pIL-12 at 0μg, 250μg, 1,000μg or 1,500μg intramuscularly by electroporation at 0, 1 and 3 months followed by intramuscular inoculation with 3.4×10(7) PFU VSV-Gag vaccine at 6 months...
September 20, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28930083/differential-patterns-of-batrachochytrium-dendrobatidis-infection-in-relict-amphibian-populations-following-severe-disease-associated-declines
#5
Steven M Whitfield, Gilbert Alvarado, Juan Abarca, Hector Zumbado, Ibrahim Zuñiga, Mark Wainwright, Jacob Kerby
Global amphibian biodiversity has declined dramatically in the past 4 decades, and many amphibian species have declined to near extinction as a result of emergence of the amphibian chytrid fungus, Batrachochytrium dendrobatidis (Bd). However, persistent or recovering populations of several amphibian species have recently been rediscovered, and such populations may illustrate how amphibian species that are highly susceptible to chytridiomycosis may survive in the presence of Bd. We conducted field surveys for Bd infection in 7 species of Costa Rican amphibians (all species that have declined to near extinction but for which isolated populations persist) to characterize infection profiles in highly Bd-susceptible amphibians post-decline...
September 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28928246/exploiting-mitochondrial-and-oxidative-vulnerabilities-with-a-synthetic-analog-of-pancratistatin-in-combination-with-piperlongumine-for-cancer-therapy
#6
Dennis Ma, Tyler Gilbert, Christopher Pignanelli, Daniel Tarade, Megan Noel, Fadi Mansour, Manika Gupta, Sabrina Ma, Jesse Ropat, Colin Curran, Sergey Vshyvenko, Tomas Hudlicky, Siyaram Pandey
Harsh adverse effects as a result of nonspecific targeting of chemotherapeutics currently pose obstacles in cancer therapy; thus, it would be invaluable to devise novel approaches to specifically target cancer cells. The natural compound pancratistatin (PST) has been shown to preferentially induce apoptosis in a variety of cancer cell types. Recently, several analogs of PST were shown to be efficacious in inducing apoptosis in a variety of aggressive cancer cell types via cancer cell mitochondrial targeting; it caused dissipation of mitochondrial membrane potential and decreased oxygen consumption, and with isolated mitochondria, it induced the release of apoptogenic factors...
September 19, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28926521/blockade-of-hla-antibody-triggered-classical-complement-activation-in-sera-from-subjects-dosed-with-the-anti-c1s-monoclonal-antibody-tnt009-results-from-a-randomized-first-in-human-phase-1-trial
#7
Jakob Mühlbacher, Bernd Jilma, Markus Wahrmann, Johann Bartko, Farsad Eskandary, Christian Schörgenhofer, Michael Schwameis, Graham C Parry, James C Gilbert, Sandip Panicker, Georg A Böhmig
BACKGROUND: Complement may play a key role in antibody-mediated rejection. A promising therapeutic approach may be classical pathway (CP) inhibition at the level of early component C1. METHODS: In this first-in-human, double-blind, randomized placebo-controlled phase 1 trial, we evaluated the safety and complement inhibitory effect of TNT009, a humanized monoclonal anti-C1s antibody. Sixty-four adult healthy volunteers received either single (n = 48; 7 consecutive cohorts, 0...
October 2017: Transplantation
https://www.readbyqxmd.com/read/28923535/creation-of-a-novel-digital-rectal-examination-evaluation-instrument-to-teach-and-assess-prostate-examination-proficiency
#8
Matthew B Clements, Karen M Schmidt, Steven E Canfield, Scott M Gilbert, Shiv R Khandelwal, Bridget F Koontz, Costas D Lallas, Stanley Liauw, Paul L Nguyen, Timothy N Showalter, Edouard J Trabulsi, Helen P Cathro, Noah S Schenkman, Tracey L Krupski
OBJECTIVE: To create a validated tool to measure digital rectal examination proficiency and aid with teaching of the examination. DESIGN: The Digital Rectal Examination Clinical Tool was created using a modified Delphi method with 5 urologists and 5 radiation oncologists. The instrument was then validated in a population of preclinical medical students examining male urological teaching associates, and clinical trainees (third- and fourth-year medical students and urology resident physicians) examining prospectively enrolled subjects...
September 8, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28922972/perioperative-anesthetic-management-of-patients-having-liver-transplantation-for-uncommon-conditions
#9
Anthony Bonavia, Justin Pachuski, Dmitri Bezinover
This review focuses on the perioperative anesthetic management of patients having liver transplantation (LT) performed for several uncommon indications or in combination with rare pathology. Conditions discussed in the article include Alagille syndrome, hypertrophic cardiomyopathy, Gilbert's syndrome, porphyria, Wilson's disease, and Budd-Chiari syndrome. In comparison to other indications, LT in these settings is infrequent because of the low incidence of these pathologies. Most of these conditions (with the exception of Gilbert syndrome) are associated with a high probability of significant perioperative complications and increased mortality and morbidity...
September 1, 2017: Seminars in Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28922075/factors-associated-with-optimal-antenatal-care-use-in-northern-region-ghana
#10
Abdulai Abubakari, Faith Agbozo, Gilbert Abotisem Abiiro
BACKGROUND: The aim of the present study was to investigate the factors associated with optimal use of antenatal care (ANC), that is, four or more times during pregnancy. METHODS: A facility-based cross-sectional survey was conducted between February and August 2014 among nursing mothers (n = 578) attending postnatal and child welfare clinics in three districts in Northern Ghana, representing urban, peri-urban and rural zones. The developed questionnaire aided the collection of information on maternal demographic characteristics, health status, household assets, and ANC attendance...
September 18, 2017: Women & Health
https://www.readbyqxmd.com/read/28921643/bridging-adult-experience-to-pediatrics-in-oncology-drug-development
#11
Ruby Leong, Hong Zhao, Gregory Reaman, Qi Liu, Yaning Wang, Clinton F Stewart, Gilbert Burckart
Pediatric drug development in the United States has grown under the current regulations made permanent by the Food and Drug Administration Safety and Innovation Act of 2012. Over 1200 pediatric studies have now been submitted to the US FDA, but there is still a high rate of failure to obtain pediatric labeling for the indication pursued. Pediatric oncology represents special problems in that the disease is most often dissimilar to any cancer found in the adult population. Therefore, the development of drug dosing in pediatric oncology patients represents a special challenge...
October 2017: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28920133/experience-with-a-community-based-multidisciplinary-memory-clinic-a-primary-care-perspective
#12
Sinead Z Wang, Gilbert Cs Tan, Xue Fei Wang, Jacqueline Giovanna De Roza, Linda Lh Lim, Nagaendran Kandiah
No abstract text is available yet for this article.
August 2017: Annals of the Academy of Medicine, Singapore
https://www.readbyqxmd.com/read/28918925/transatlantic-editorial-thoracic-surgeons-need-recognition-of-competence-in-thoracic-oncology
#13
EDITORIAL
Gilbert Massard, Mara B Antonoff, Julie-Lynn Noel, Alessandro Brunelli, Farhood Farjah, Michael Lanuti, Dirk Van Raemdonck
No abstract text is available yet for this article.
October 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28917888/structure-of-cellulose-microfibrils-in-mature-cotton-fibres
#14
Marta Martínez-Sanz, Filomena Pettolino, Bernadine Flanagan, Michael J Gidley, Elliot P Gilbert
The structure of cellulose microfibrils in mature cotton fibres from three varieties - Gossypium hirsutum, G. barbadense and G. arboreum - has been investigated by a multi-technique approach combining small angle scattering techniques with spectroscopy and diffraction. Cellulose microfibrils present a Iβ-rich crystalline structure with limited surface disorder. Small angle scattering (SAXS and SANS) data have been successfully fitted using a core-shell model and the results obtained indicate that the cellulose microfibrils, formed by the association of 2-3 elementary fibrils, are composed of a ca...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917865/physicochemical-and-structural-properties-of-pregelatinized-starch-prepared-by-improved-extrusion-cooking-technology
#15
Yunfei Liu, Jun Chen, Shunjing Luo, Cheng Li, Jiangping Ye, Chengmei Liu, Robert G Gilbert
Pregelatinized starch was made from indica rice starch using a so-called "improved extrusion cooking technology" (IECT) under 30%-70% moisture content. IECT-pregelatinized starch (IPS) had higher water solubility and water absorbability compared to native starch at low temperature. For pasting properties, the breakdown and setback viscosities of IPS were significantly (p<0.05) lower than native starch, suggesting improved gel stability and reduced short-term retrogradation. The rice starch granules lost their integrity in IPS, and formed a honeycomb-like structure with increased moisture content in the raw material...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917813/structural-impacts-on-the-timing-and-amplitude-of-the-negative-bold-response
#16
David Provencher, Alexandre Bizeau, Guillaume Gilbert, Yves Bérubé-Lauzière, Kevin Whittingstall
The positive (PBR) and negative BOLD responses (NBR) arising in task-fMRI display varying magnitudes and dynamics across voxels. While the effects of structure, particularly of veins, on the PBR have been studied, little is known of NBR-structure relationships. Like the PBR, the NBR is often used as a surrogate marker of neuronal activation in both basic and clinical research and assessing its relationship with cortical structure may help interpret group differences. We therefore investigated how local structure affects BOLD amplitude and timing in PBR and NBR areas using multi-band fMRI during visual stimulation to obtain high temporal resolution (TR=0...
September 13, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28917763/expanded-uncertainty-associated-with-determination-of-isotope-enrichment-factors-comparison-of-two-point-calculation-and-rayleigh-plot
#17
Maxime Julien, Alexis Gilbert, Keita Yamada, Richard J Robins, Patrick Höhener, Naohiro Yoshida, Gérald S Remaud
The enrichment factor (ε) is a common way to express Isotope Effects (IEs) associated with a phenomenon. Many studies determine ε using a Rayleigh-plot, which needs multiple data points. More recent articles describe an alternative method using the Rayleigh equation that allows the determination of ε using only one experimental point, but this method is often subject to controversy. However, a calculation method using two points (one experimental point and one at t0) should lead to the same results because the calculation is derived from the Rayleigh equation...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917598/matrix-effect-free-quantitative-liquid-chromatography-mass-spectrometry-analysis-in-complex-matrices-using-nanoflow-lc-with-integrated-emitter-tip-and-high-dilution-factors
#18
David Moreno-González, Jaime Alcántara-Durán, Bienvenida Gilbert-López, Juan F García-Reyes, Antonio Molina-Díaz
Matrix effects are probably the Achilles heel of most quantitative liquid chromatography mass spectrometry (LC-MS) methods based on electrospray ionization. This work reports the evaluation of matrix effects in challenging matrices such as food extracts, human urine or wastewater at different dilution factors using nanoflow liquid chromatography-high resolution mass spectrometry (LC-MS). For this purpose, a suite of representative low-molecular weight compounds such as pesticides, drugs of abuse, sport drugs or environmental contaminants were selected...
September 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28917505/association-of-self-reported-discrimination-and-suicide-ideation-in-older-chinese-americans
#19
Lydia W Li, Gilbert C Gee, XinQi Dong
OBJECTIVE: This study examines racial discrimination as a potential novel risk factor for suicide ideation among older Chinese Americans. DESIGN: In a cross-sectional analysis, this study drew on data collected in the Population-based Study of Chinese Elderly in Chicago on Chinese older adults age 60 + in the Greater Chicago area (N = 3,157). Thirty-day suicide ideation was a dichotomous variable, derived from items of the Physical Health Questionnaire and the Geriatric Mental State Examination-Version A...
August 17, 2017: American Journal of Geriatric Psychiatry
https://www.readbyqxmd.com/read/28917041/modification-of-selenoprotein-mrnas-by-cap-tri-methylation
#20
Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang
Several selenoprotein mRNAs undergo 5' cap maturation events whereby their classical monomethylated m(7)G cap becomes trimethylated (m3(2,2,7)G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunoprecipitation methods for the detection of endogenous m3(2,2,7)G-capped selenoprotein mRNAs from total cell extracts or after polysome fractionation of cytoplasmic extracts. We have also developed a method for the in vitro cap hypermethylation of selenoprotein mRNA transcripts using purified Tgs1 enzyme...
2018: Methods in Molecular Biology
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