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https://www.readbyqxmd.com/read/28634638/co-existence-of-anaerobic-ammonium-oxidation-bacteria-and-denitrifying-anaerobic-methane-oxidation-bacteria-in-sewage-sludge-community-diversity-and-seasonal-dynamics
#1
Sai Xu, Wenjing Lu, Muhammad Farooq Mustafa, Luis Miguel Caicedo, Hanwen Guo, Xindi Fu, Hongtao Wang
Anaerobic ammonium oxidation (ANAMMOX) and denitrifying anaerobic methane oxidation (DAMO) have been recently discovered as relevant processes in the carbon and nitrogen cycles of wastewater treatment plants. In this study, the seasonal dynamics of ANAMMOX and DAMO bacterial community structures and their abundance in sewage sludge collected from wastewater treatment plants were analysed. Results indicated that ANAMMOX and DAMO bacteria co-existed in sewage sludge in different seasons and their abundance was positively correlated (P < 0...
June 20, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28633946/microplastics-in-gut-contents-of-coastal-freshwater-fish-from-r%C3%A3-o-de-la-plata-estuary
#2
Rocío S Pazos, Tomás Maiztegui, Darío C Colautti, Ariel H Paracampo, Nora Gómez
The presence of microplastics (MPs) in gut contents of coastal freshwater fish of the Rio de la Plata estuary was studied. Samples were taken in six sites where 87 fish belonging to 11 species and four feeding habits were captured. Presence of MPs was verified in the 100% of fish. The fibres represented the 96% of MPs found. The number of MPs in gut contents was significantly higher close to sewage discharge. There was not found relationship between number of MPs and fish length, weight or feeding habit. The spatial differences in mean number of MPs in fish observed in this study, suggest that environmental availability of MPs could be of great importance to explain the differences found among sampling sites analysed...
June 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28633517/biomethanation-of-sewage-sludge-with-food-waste-leachate-via-co-digestion
#3
Jingyeong Shin, Young Beom Kim, Jong Hun Jeon, Sangki Choi, In Kyu Park, Young Mo Kim
Anaerobic mono- and co-digestion of sewage sludge (SS) and food waste leachate (FWL) were performed by assessing methane production and characterizing microbial communities. Anaerobic digestion (AD) of waste activated sludge (WAS) alone produced the lowest methane production (281 mL-CH4), but an approximately 80% increase in methane production was achieved via co-digestion of WAS and FWL (506 mL-CH4). There were less differences in the diversity of bacterial communities in anaerobic digesters, while archaeal (ARC) and bacterial (BAC) amounts reflected AD's performance...
June 21, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28629812/benthic-foraminifera-for-heavy-metal-pollution-monitoring-a-case-study-from-burullus-lagoon-of-egypt
#4
O H Orabi, A A El-Badry, A M Badr-ElDin
Sources of heavy metals pollution in the Burullus Lagoon include phosphate fertilizers, sewage and oil spills from fishing boats. The benthic species identified in this lagoon are Adelosina carinata striata (Wiesner), Quinqueloculina bosciana (d'Orbigny), Quinqueloculina seminulum (Linnaeus), Ammonia tepida (Cushman), Elphidium excavatum (Terquem). A. tepida is the most dominant species in the lagoon. It constitutes more than 97% of the total benthic foraminiferal assemblages reflecting tolerance to the very low salinity in the Burullus Lagoon...
June 16, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28628741/amplicon-competition-enables-end-point-quantitation-of-nucleic-acids-following-isothermal-amplification
#5
Yu Sherry Jiang, Apollo Stacy, Marvin Whiteley, Andrew D Ellington, Sanchita Bhadra
It is inherently difficult to quantitate nucleic acid analytes with most isothermal amplification assays. We have now developed loop-mediated isothermal amplification (LAMP) reactions in which competition between defined numbers of 'false' and 'true' amplicons leads to order of magnitude quantitation via a single end-point determination. These thresholded LAMP reactions were successfully used to directly and quantitatively estimate the numbers of nucleic acids in complex biospecimens, including directly from cells and in sewage, with the values obtained closely correlating with qPCR quantitations...
June 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28625997/quantifying-vibrio-cholerae-enterotoxicity-in-a-zebrafish-infection-model
#6
Kristie C Mitchell, Paul Breen, Sarah Britton, Melody N Neely, Jeffrey H Withey
Vibrio cholerae is the etiological agent of cholera, an acute intestinal infection in humans characterized by voluminous watery diarrhea. Cholera is spread through ingestion of contaminated food or water, primarily in developing countries that lack the proper infrastructure for proper water and sewage treatment. Vibrio cholerae is an aquatic bacterium that inhabits coastal and estuarine areas, and is known to have several environmental reservoirs, including fish. Our laboratory has recently described the use of the zebrafish as a new animal model for the study of V...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28625705/the-feasibility-of-improved-live-dead-distinction-in-qpcr-based-microbial-source-tracking
#7
Seidel Laura, Strathmann Martin, Nocker Andreas
PCR-based microbial source tracking (MST) has become a useful tool to identify dominant sources of fecal pollution in water. The method has previously been successfully combined with viability PCR (using propidium monoazide) allowing the preferential detection of membrane-intact bacteria. This study aimed at further improving the selectivity for intact cells when targeting host-specific markers in Bacteroidales bacteria. One approach was to increase amplicon sizes that had been shown to be useful for other applications of viability PCR...
June 15, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28624741/reduced-sludge-growth-at-high-bulk-liquor-dissolved-oxygen-induced-by-increased-secondary-cell-maintenance
#8
Anwar Khursheed, Vinay Kumar Tyagi, Rubia Z Gaur, Meena Kumari Sharma, Abid Ali Khan, A A Kazmi
Sludge reduction by physico-chemical methods results in the buildup of chemicals, which may require further treatment. Owing these reasons various biologically sustainable methods of sludge reduction including the application of high oxygenation have been successfully tested. Experiments on actual sewage in two lab-scale sequencing batch reactors (SBRs) were conducted under normal (1.5-2.5 mgDO/L) and high dissolved oxygen (DO) (HDO: 3-6.5 mgDO/L) regimes. It was observed that microorganism allocated substrate between maintenance and growth in the form of maintenance coefficient...
June 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28624733/gastrointestinal-illness-linked-to-incidents-in-drinking-water-distribution-networks-in-sweden
#9
Melle Säve-Söderbergh, John Bylund, Annika Malm, Magnus Simonsson, Jonas Toljander
During recent years, knowledge gaps on drinking water-related gastrointestinal illness have been identified, especially for non-epidemic cases. Pathogen contamination of drinking water during distribution has been suggested to contribute to these cases, but the risk factors are not yet fully understood. During 2014-2015, we conducted an epidemiological study in five municipalities in Sweden, to assess whether incidents in the drinking water distribution system influence the risk of gastrointestinal illness...
June 5, 2017: Water Research
https://www.readbyqxmd.com/read/28624731/optimizing-mixing-mode-and-intensity-to-prevent-sludge-flotation-in-sulfidogenic-anaerobic-sludge-bed-reactors
#10
Bo Wang, Di Wu, George A Ekama, Hao Huang, Hui Lu, Guang-Hao Chen
Sludge flotation is a notorious problem in anaerobic wastewater treatment that can occur under various operational conditions and even cause the anaerobic process to completely fail. Despite having been documented for over three decades, its causes and remedies remain elusive, particularly for low-gas-production anaerobic processes such as sulfidogenic and anammox processes. This paper systematically studies sludge flotation in an anaerobic sulfidogenic process for saline domestic sewage treatment. Three lab-scale sulfidogenic reactors were operated in parallel with different modes of mixing (hydraulic, mechanical and pneumatic) at various mixing intensity levels at shear rates ranging from 0...
June 7, 2017: Water Research
https://www.readbyqxmd.com/read/28623653/miniaturized-temperature-controlled-planar-chromatography-micro-tlc-as-a-versatile-technique-for-fast-screening-of-micropollutants-and-biomarkers-derived-from-surface-water-ecosystems-and-during-technological-processes-of-wastewater-treatment
#11
Magdalena M Ślączka-Wilk, Elżbieta Włodarczyk, Aleksandra Kaleniecka, Paweł K Zarzycki
There is increasing interest in the development of simple analytical systems enabling the fast screening of target components in complex samples. A number of newly invented protocols are based on quasi separation techniques involving microfluidic paper-based analytical devices and/or micro total analysis systems. Under such conditions, the quantification of target components can be performed mainly due to selective detection. The main goal of this paper is to demonstrate that miniaturized planar chromatography has the capability to work as an efficient separation and quantification tool for the analysis of multiple targets within complex environmental samples isolated and concentrated using an optimized SPE method...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623572/nutritional-fe-mn-ni-and-cr-and-growth-responses-of-rice-plant-affected-by-perennial-application-of-two-bio-solids
#12
Seyed Majid Mousavi, Mohammad Ali Bahmanyar, Hemmatollah Pirdashti, Salahedin Moradi
Trace toxic elements often restrict the land application of different bio-solids in agriculture. In order to evaluate the separate influence of the municipal solid waste compost (MSW), sewage sludge (SS) and combined application with inorganic fertilizers (chemical fertilizer (CF)) on nutritional (Fe, Mn, Ni, and Cr) and growth responses of rice plant, a research was conducted on paddy soil from 2013 to 2015. Obtained results showed that SS levels were superior to MSW in most studied traits. The maximum chlorophyll content (46...
July 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28623022/heterogeneity-of-zeolite-combined-with-biochar-properties-as-a-function-of-sewage-sludge-composting-and-production-of-nutrient-rich-compost
#13
Mukesh Kumar Awasthi, Meijing Wang, Ashok Pandey, Hongyu Chen, Sanjeev Kumar Awasthi, Quan Wang, Xiuna Ren, Altaf Hussain Lahori, Dong-Sheng Li, Ronghua Li, Zengqiang Zhang
In the present study, biochar combined with a higher dosage of zeolite (Z) and biochar (B) alone were applied as additives for dewatered fresh sewage sludge (DFSS) composting using 130-L working volume lab-scale reactors. We first observed that the addition of a mixture of B and Z to DFSS equivalent to 12%B+10% (Z-1), 15% (Z-2) and 30% (Z-3) zeolite (dry weight basis) worked synergistically as an amendment and increased the composting efficiency compared with a treatment of 12%B alone amended and a control without any amendment...
June 13, 2017: Waste Management
https://www.readbyqxmd.com/read/28620851/changes-of-erythrocyte-metric-parameters-in-pelophylax-ridibundus-amphibia-anura-ranidae-inhabiting-water-bodies-with-different-types-of-anthropogenic-pollution-in-southern-bulgaria
#14
Zhivko Zhelev, Georgi Popgeorgiev, Ivan Ivanov, Peter Boyadzhiev
The article presents the basic erythrocyte-metric parameters: cell length (EL) and width (EW), EL/EW, erythrocyte size (ES), nucleus length (NL) and width (NW), NL/NW, nucleus size (NS) and nucleocytoplasmic ratio (NS/ES) in the wild populations of marsh frogs Pelophylax ridibundus from five water bodies in Southern Bulgaria (two rivers and three reservoirs) with different degrees and types of anthropogenic pollution (less disrupted water basins, domestic sewage pollution and heavy metal pollution). The changes in erythrocyte-metric parameters depend on concentrations and types of toxicant and, to a lesser extent, on the type of water basin...
June 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28620817/effect-of-biosolid-hydrochar-on-toxicity-to-earthworms-and-brine-shrimp
#15
Tatiane Medeiros Melo, Michael Bottlinger, Elke Schulz, Wilson Mozena Leandro, Adelmo Menezes de Aguiar Filho, Yong Sik Ok, Jörg Rinklebe
The hydrothermal carbonization of sewage sludge has been studied as an alternative technique for the conversion of sewage sludge into value-added products, such as soil amendments. We tested the toxicity of biosolid hydrochar (Sewchar) to earthworms. Additionally, the toxicity of Sewchar process water filtrate with and without pH adjustment was assessed, using brine shrimps as a model organism. For a Sewchar application of 40 Mg ha(-1), the earthworms significantly preferred the side of the vessel with the reference soil (control) over side of the vessel with the Sewchar treatments...
June 15, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28620370/environmental-t4-family-bacteriophages-evolve-to-escape-abortive-infection-via-multiple-routes-in-a-bacterial-host-employing-altruistic-suicide-through-type-iii-toxin-antitoxin-systems
#16
Bihe Chen, Chidiebere Akusobi, Xinzhe Fang, George P C Salmond
Abortive infection is an anti-phage mechanism employed by a bacterium to initiate its own death upon phage infection. This reduces, or eliminates, production of viral progeny and protects clonal siblings in the bacterial population by an act akin to an "altruistic suicide." Abortive infection can be mediated by a Type III toxin-antitoxin system called ToxINPa consisting of an endoribonuclease toxin and RNA antitoxin. ToxINPa is a heterohexameric quaternary complex in which pseudoknotted RNA inhibits the toxicity of the toxin until infection by certain phages causes destabilization of ToxINPa, leading to bacteriostasis and, eventually, lethality...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28619635/production-and-characterization-of-polyhydroxyalkanoic-acid-from-bacillus-thuringiensis-using-different-carbon-substrates
#17
Olubusola A Odeniyi, Olakunle J Adeola
Polyhydroxyalkanoic acids (PHA) are biodegradable microbially synthesised polymers which can act as alternatives to conventional petrochemical based plastics. Bacterial isolates from soil and sewage samples were screened for the ability to accumulate PHA using Sudan Black B dye, and growth conditions of the best PHA accumulating bacterium was optimized. The PHA produced was characterized by FTIR spectroscopy. Eight PHA-producing bacteria were isolated and isolate SBC4 which had the highest percentage accumulation of 30% was genotypically identified as Bacillus thuringiensis and selected for further studies...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28618659/projected-impact-of-climate-change-and-chemical-emissions-on-the-water-quality-of-the-european-rivers-rhine-and-meuse-a-drinking-water-perspective
#18
Rosa M A Sjerps, Thomas L Ter Laak, Gertjan J J G Zwolsman
Low river discharges of the rivers Rhine and Meuse are expected to occur more often and more prolonged in a changing climate. During these dry periods the dilution of point sources such as sewage effluents is reduced leading to a decline in chemical water quality. This study projects chemical water quality of the rivers Rhine and Meuse in the year 2050, based on projections of chemical emissions and two climate scenarios: moderate and fast climate change. It focuses on specific compounds known to be relevant to drinking water production, i...
June 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28617280/two-different-purification-systems-at-istanbul-tuzla-and-adana-kozan-their-characterizations
#19
Abdullah Yinanç
In most developed countries, wastewater treatment plants are presently utilizing efficient purification technology meeting the sufficiency requirements of quality and quantity. However, in developing countries, the number of wastewater treatment plants is limited. As a result, the establishment of a connection between the wastewater collection system and wastewater purification plants is increasingly becoming a top priority for researchers. The aim of this article is to analyze wastewater collection and purification systems and establish the links between these two processes...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28611503/exploiting-phosphate-starved-cells-of-scenedesmus-sp-for-the-treatment-of-raw-sewage
#20
Swati Yewalkar-Kulkarni, Gayatri Gera, Sanjay Nene, Kiran Pandare, Bhaskar Kulkarni, Sanjay Kamble
Phosphate depletion is one of the favorable ways to enhance the sewage water treatment with the algae, however, detailed information is essential with respect to internal phosphate concentration and physiology of the algae. The growth rate of the phosphate-starved Scenedesmus cells was reduced drastically after 48 h. Indicating cells entered in the stationary phase of the growth cycle. Fourier Transform Infrared analysis of phosphate-starved Scenedesmus cells showed the reduction in internal phosphate concentration and an increase in carbohydrate/phosphate and carbohydrate/lipid ratio...
June 2017: Indian Journal of Microbiology
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