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https://www.readbyqxmd.com/read/29775115/immunofluorescent-localization-of-non-myelinating-schwann-cells-and-their-interactions-with-immune-cells-in-mouse-thymus
#1
Dailun Hu, Philip K Nicholls, Changfu Yin, Khama Kelman, Qionglan Yuan, Wayne K Greene, Zhongli Shi, Bin Ma
The thymus is innervated by sympathetic/parasympathetic nerve fibers from the peripheral nervous system (PNS), suggesting a neural regulation of thymic function including T-cell development. Despite some published studies, data on the innervation and nerve-immune interaction inside the thymus remain limited. In the present study, we used immunofluorescent staining of glial fibrillary acidic protein (GFAP) coupled with confocal microscopy/three-dimensional (3D) reconstruction to reveal the distribution of non-myelinating Schwann cells (NMSC) and their interactions with immune cells inside mouse thymus...
May 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29770639/effect-of-cataract-grade-according-to-wide-field-fundus-images-on-measurement-of-macular-thickness-in-cataract-patients
#2
Mingue Kim, Youngsub Eom, Jong Suk Song, Hyo Myung Kim
PURPOSE: To investigate the effects of cataract grade based on wide-field fundus imaging on macular thickness measured by spectral domain optical coherence tomography (SD-OCT) and its signal-to-noise ratio (SNR). METHODS: Two hundred cataract patients (200 eyes) with preoperative measurements by wide-field fundus imaging and macular SD-OCT were enrolled. Cataract severity was graded from 1 to 4 according to the degree of macular obscuring by cataract artifact in fundus photo images...
May 15, 2018: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/29755825/bilateral-asymmetric-rhegmatogenous-retinal-detachment-in-a-patient-with-stickler-syndrome
#3
Caner Öztürk, Almila Sarıgül Sezenöz, Gürsel Yılmaz, İmren Akkoyun
Here we present the long-term anatomical and visual outcomes of bilateral asymmetric rhegmatogenous retinal detachment repair in a patient with Stickler syndrome. A 17-year-old girl presented with decreased visual acuity in both eyes for more than one year. Her best-corrected visual acuity (BCVA) was 0.1 in the right eye and 0.05 in the left eye. Slit-lamp anterior segment examination revealed subcapsular cataract in both eyes. Fundus examination showed bilateral rhegmatogenous retinal detachment, chronic retinal detachment accompanied by multiple retinal holes, tears and membranous fibrillary vitreous in the peripheral retina...
April 2018: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29748647/isolation-and-functional-assessment-of-mouse-skeletal-stem-cell-lineage
#4
Gunsagar S Gulati, Matthew P Murphy, Owen Marecic, Michael Lopez, Rachel E Brewer, Lauren S Koepke, Anoop Manjunath, Ryan C Ransom, Ankit Salhotra, Irving L Weissman, Michael T Longaker, Charles K F Chan
There are limited methods available to study skeletal stem, progenitor, and progeny cell activity in normal and diseased contexts. Most protocols for skeletal stem cell isolation are based on the extent to which cells adhere to plastic or whether they express a limited repertoire of surface markers. Here, we describe a flow cytometry-based approach that does not require in vitro selection and that uses eight surface markers to distinguish and isolate mouse skeletal stem cells (mSSCs); bone, cartilage, and stromal progenitors (mBCSPs); and five downstream differentiated subtypes, including chondroprogenitors, two types of osteoprogenitors, and two types of hematopoiesis-supportive stroma...
June 2018: Nature Protocols
https://www.readbyqxmd.com/read/29732041/a-rare-case-of-hepatic-subcapsular-biloma-after-laparoscopic-cholecystectomy-and-subsequent-endoscopic-retrograde-cholangiopancreatography
#5
Alimohammad Jafari
No abstract text is available yet for this article.
2018: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/29714816/sentinel-lymph-node-metastasis-in-primary-cutaneous-basosquamous-carcinoma-a-cross-sectional-study
#6
Despoina D Kakagia, Polyxeni Zapandioti, Grigoris Trypsiannis, Alexandra N Grekou, Dimosthenis Tsoutsos
BACKGROUND AND OBJECTIVES: Basosquamous carcinoma (BSC) is a rare, biologically aggressive tumor. This cross-sectional study aims to define risk factors for subclinical nodal metastasis in primary BSC, and identify the patients who would benefit from routine sentinel node biopsy (SLNB) as part of the initial management. METHODS: A total of 142 patients, with histologically proven BSC without palpable lymph nodes, underwent SLNB after the initial excision. Clinicopathological features and demographics were analyzed between the patients with detected micrometastasis (SLNM) and those with negative SLN...
May 1, 2018: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29708780/bleeding-complications-after-percutaneous-liver-biopsy-do-subcapsular-lesions-pose-a-higher-risk
#7
Theodora A Potretzke, Lauren J Saling, William D Middleton, Kathryn A Robinson
OBJECTIVE: The purpose of this study is to determine the incidence of moderate and severe bleeding complications after subcapsular liver mass biopsy and compare it with the incidences of such complications after nonsubcapsular liver mass biopsy and after random biopsy. MATERIALS AND METHODS: This retrospective review of ultrasound-guided liver biopsies was performed between January 2006 and February 2015. Biopsy type, needle size, the number of needle passes, and mass size and depth were recorded...
April 30, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29705099/ultraviolet-radiation-exposure-triggers-neurokinin-1-receptor-upregulation-in-ocular-tissues-in-vivo
#8
Janine Gross, Alfred R Wegener, Martin Kronschlaeger, Frank G Holz, Carl-Ludwig Schönfeld, Linda M Meyer
The purpose of this study was to investigate the neurokinin receptor-1 (NKR-1) protein expression in ocular tissues before and after supra-cataract threshold ultraviolet radiation (UVR-B peak at 312 nm) exposure in vivo in a mouse model. Six-week-old C57Bl/6 mice were unilaterally exposed to a single (2.9 kJ/m2 ) and an above 3-fold UVR-B cataract threshold dose (9.4 kJ/m2 ) of UVR. UVR-exposure (λpeak = 312 nm) was performed in mydriasis using a Bio-Spectra exposure system. After latency periods of 3 and 7 days, eyes were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned and stained with fluorescence coupled antibody for NKR-1 and DAPI for cell nuclei staining...
April 26, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29699420/analysis-of-preventability-of-hypertensive-disorder-in-pregnancy-related-maternal-death-using-the-nationwide-registration-system-of-maternal-deaths-in-japan
#9
Shinji Katsuragi, Hiroaki Tanaka, Junichi Hasegawa, Masamitsu Nakamura, Naohiro Kanayama, Masahiko Nakata, Takeshi Murakoshi, Jun Yoshimatsu, Kazuhiro Osato, Kayo Tanaka, Akihiko Sekizawa, Isamu Ishiwata, Tomoaki Ikeda
OBJECTIVE: Hypertensive disorder of pregnancy (HDP) is a major cause of maternal death. The goal of this study was to investigate factors associated with maternal death due to HDP. STUDY DESIGN: HDP-related maternal deaths in Japan reported to the Committee of the Ministry of Health, Labor and Welfare from 2010 to 2015 were examined. RESULTS: Out of 47 cases of HDP, 30 were identified as the major cause of maternal death. The median maternal age was 34 years (range 24-45) and the mortality in women aged ≥40 years was seven times higher that than in women aged <34 years...
April 26, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29695215/high-background-incidence-of-spontaneous-subcapsular-adrenal-gland-hyperplasia-of-tg-rash2-mice-used-in-26-week-carcinogenicity-studies
#10
Molly H Boyle, Madhav G Paranjpe, Dianne M Creasy
The Tg.rasH2 model was accepted by regulatory agencies worldwide for 26-week carcinogenicity assays as an alternative to the standard 2-year assays in conventional mice in 2003. Several references documenting spontaneous nonneoplastic findings and incidences of spontaneous tumors in the Tg.rasH2 mice have been published. The purpose of this publication is to add adrenal gland subcapsular hyperplasia to the database pertaining to spontaneous lesions noted in Tg.rasH2 mice, review physiology related to this finding, and discuss its significance...
January 1, 2018: Toxicologic Pathology
https://www.readbyqxmd.com/read/29682903/cataract-frequency-and-subtypes-involved-in-workers-assessed-for-their-solar-radiation-exposure-a-systematic-review
#11
REVIEW
Alberto Modenese, Fabriziomaria Gobba
Cataract is currently the primary cause of blindness worldwide, and one of its main risk factors is solar ultraviolet radiation exposure. According to the localization of lens opacities, three main subtypes of cataract are recognized: nuclear, cortical and posterior subcapsular cataract. One of the main determinants of individual long-term solar radiation exposure is outdoor work. We systematically reviewed scientific literature from the last 20 years to update the recent development of research on the risk of cataract in outdoor workers and on the specific subtypes involved, also investigating the methods applied to evaluate the occupational risk...
April 16, 2018: Acta Ophthalmologica
https://www.readbyqxmd.com/read/29676345/bilateral-subcapsular-orchiectomy-versus-bilateral-total-orchiectomy-comparison-of-the-quality-of-life-post-orchiectomy
#12
Dubem Ejikeme Orakwe, Kehinde Habeeb Tijani, Emmauel Ajibola Jeje, Moses Adebisi Ogunjimi, Wale Ojewola Rufus, Taiwo Opeyemi Alabi
Objective: Bilateral subcapsular orchiectomy (BSO) is said to be more aesthetic and psychologically satisfying when compared to bilateral total orchiectomy (BTO). This study compared the quality of life (QoL) of men with advanced prostate cancer who had BTO to those who had BSO, with an emphasis on their perception of self or identity as a man. Subjects and Methods: Sixty-one patients with advanced prostate cancer opting for bilateral orchiectomy were recruited...
January 2018: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/29669766/rare-case-of-hepatic-haematoma-following-endoscopic-retrograde-cholangiopancreatography
#13
Teresa Vieira Caroço, João Mendes Louro, Maria Inês Coelho, Carlos Eduardo Costa Almeida
Endoscopic retrograde cholangiopancreatography (ERCP) is a commonly used diagnostic and therapeutic technique but it is not free of complications. Subcapsular hepatic haematoma is a rare but potentially fatal complication. A 71-years-old male patient resorted to the emergency department 36 hours after an uneventful ERCP presenting with severe abdominal pain and haemoglobin drop. Abdominal ultrasound and CT scan diagnosed a subcapsular hepatic haematoma. The patient was haemodynamically normal and was successfully treated with a conservative management...
April 18, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29660398/molecular-phenotypes-of-the-human-kidney-myoid-stromal-cells-telocytes-and-myoepithelial-cells
#14
M C Rusu, L Mogoantă, F Pop, M A Dobra
The connective stromal and epithelial compartments of the kidney have regenerative potential and phenotypic flexibility. A few studies have shown that cells appertaining to both compartments can exhibit myoid phenotypes. The purpose of our study was to investigate the myoid pattern of kidney and its association with the kidney niches containing stromal cells/telocytes (SC/TCs). We performed an immunohistochemical study using a panel of endothelial, myoid, mesenchymal and stem/progenitor markers, namely CD31, CD34, CD105 (endoglin), CD117/c-kit, nestin, desmin, α-smooth muscle actin (α-SMA) and the heavy chain of smooth muscle myosin (SMM)...
April 13, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29659397/scintigraphic-and-sonographic-findings-of-subcapsular-cortical-necrosis-in-a-transplanted-kidney
#15
Bulent Yazici, Emrah Gunay, Aysegül Akgün, Sait Sen, Suha Sureyya Ozbek
Renal transplant scintigraphy, performed in a 23-year-old man who had renal graft from a living donor, showed nearly normal perfusion and moderately low function of the graft. But the margins of the graft were blurred, and it was interestingly appearing enlarged late in the study. Ultrasound demonstrated a hypoechoic rim surrounding the whole kidney. Biopsy revealed necrosis besides normal cortical tissue. Necrosis was thought to be limited to the subcapsular cortical area. It should be considered that these scintigraphic findings could be due to greatly reduced and delayed perfusion of edematous subcapsular necrotic area of the graft even in normal perfusion images...
April 13, 2018: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/29643683/postrenal-transplant-allograft-page-kidney-identified-and-salvaged-using-99m-tc-diethylenetriaminepentaacetic-acid-renogram-and-single-photon-emission-computed-tomography
#16
Aashish Gambhir, Indirani Elangovan, Shelley Simon, Avani Jain
99m Tc diethylenetriaminepentaacetic acid (DTPA) renogram is a commonly performed evaluation postrenal transplant to assess graft function and for early detection of suspected immediate and late transplant-associated complications. Although several modalities can be utilized to detect perinephric collection in posttransplant period, the utility of 99m Tc DTPA single-photon emission-computed tomography (SPECT-CT) is not recognized. Herein, we discuss the incremental role of seldom considered SPECT-CT in early detection, leading to timely appropriate management and graft salvage in a case of posttransplant deteriorating renal allograft as a result of subcapsular hematoma...
April 2018: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
https://www.readbyqxmd.com/read/29626052/association-between-serum-lipids-concentration-and-patients-with-age-related-cataract-in-china-a-cross-sectional-case-control-study
#17
Shengjie Li, Danhui Li, Yudong Zhang, Jisen Teng, Mingxi Shao, Wenjun Cao
OBJECTIVE: Obesity and a high-fat diet have been found to be associated with an increased risk of age-related cataract (ARC). Thus, this study aimed to investigate whether serum lipid levels are associated with the incidence of ARC. DESIGN: Cross-sectional, case-control study. SETTING: EyeandENT Hospital of Fudan University, Shanghai, China. PARTICIPANTS: A total of 219 ARC (male=94, female=125) subjects and 218 (male=110, female=118) normal control subjects were recruited in this study...
April 5, 2018: BMJ Open
https://www.readbyqxmd.com/read/29622345/early-islets-and-mesenchyme-from-an-injured-adult-pancreas-improve-syngeneic-engraftments-and-islet-graft-function-in-diabetic-rats
#18
Venant Tchokonte-Nana, Juziel Kampando Manda
A decrease in mass of isografts and a decline in islet function are major challenges in islet transplantations. Despite this, transplantation of 84 h harvested pancreatic duct ligation (PDL) tissues have been shown to have the same functional ability to foetal pancreata, but there was only 40% success in reverting hyperglycaemia. We tested the potential of early islets with mesenchymal stromal cells (MSCs) to promote isogeneic grafts survival and to restore normoglycemia in diabetic rats, in comparison with late islets...
April 2, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29613981/phacomorphic-angle-closure-following-silicone-oil-tamponade-in-a-pediatric-patient
#19
Victoria C Leung, Simon Fung, Rajeev Muni, Asim Ali
PURPOSE: To report a case of phacomorphic glaucoma following retinal detachment repair with silicone oil in a pediatric patient. STUDY DESIGN: Case report. METHODS: A chart review was conducted at St. Michael's Hospital and The Hospital for Sick Children (Toronto, Canada), where the patient received ophthalmic care from July 28, 2015 onwards. RESULTS: A 14 year-old boy with a history of traumatic retinal detachment and proliferative vitreoretinopathy in the left eye, requiring two pars plana vitrectomies, membrane peel, and silicone oil tamponade, presented with one-day history of decreased vision in the left eye, severe headache, nausea, and vomiting...
April 2, 2018: Journal of Glaucoma
https://www.readbyqxmd.com/read/29611160/stress-induced-changes-in-the-aged-rat-adrenal-cortex-histological-and-histomorphometric-study
#20
Sherif Mohamed Zaki, Fayza Abdelraouf Abdelgawad, Ehab Abdel Aziz El-Shaarawy, Rasha Abdel Khalek Radwan, Basma Emad Aboul-Hoda
BACKGROUND: Stress exposure exerts direct effects on the morphology and functionality of the adrenal cortex. In addition, aging effects growth, differentiation, apoptosis and cellularity of the cortex. The missing data is the combined effect of stress and aging on the adrenal cortex. The aim of this study was to demonstrate the structural changes in the adrenal cortex following the exposure to stress in the adult and aged albino rats. MATERIAL AND METHODS: 40 rats were divided into groups I, II (adult and senile)...
April 3, 2018: Folia Morphologica (Warsz)
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