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Burkholderia cepacia complex

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https://www.readbyqxmd.com/read/28432094/the-use-of-synthetic-hybrid-strains-to-determine-the-role-of-replicon-3-in-virulence-of-the-burkholderia-cepacia-complex
#1
Kirsty Agnoli, Roman Freitag, Margarida C Gomes, Christian Jenul, Angela Suppiger, Olga Mannweiler, Carmen Frauenknecht, Daniel Janser, Annette C Vergunst, Leo Eberl
The Burkholderia cepacia complex (Bcc) displays a wealth of metabolic diversity with great biotechnological potential, but the utilisation of these bacteria is limited by their opportunistic pathogenicity to humans. The third replicon of the Bcc, megaplasmid pC3 (0.5 to 1.4 Mb, previously chromosome 3), is important for various phenotypes, including virulence, antifungal and proteolytic activities, and the utilization of certain substrates. Approximately half of plasmid pC3 is well-conserved throughout sequenced Bcc members, while the other half is not...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28431848/epidemiology-of-device-associated-infections-in-an-intensive-care-unit-of-a-teaching-hospital-in-nepal-a-prospective-surveillance-study-from-a-developing-country
#2
Narayan Prasad Parajuli, Subhash Prasad Acharya, Santosh Dahal, Jaya Prasad Singh, Shyam Kumar Mishra, Hari Prasad Kattel, Basista Prasad Rijal, Bharat Mani Pokhrel
BACKGROUND: Device-associated health care-acquired infections (DA-HAIs) in intensive care unit patients are a major cause of morbidity, mortality, and increased health care costs. METHODS: A prospective, structured clinicomicrobiological surveillance was carried out for 3 common DA-HAIs: ventilator-associated pneumonia (VAP), central line-associated bloodstream infection (CLABSI), and catheter-associated urinary tract infection (CAUTI) present in the patients of an intensive care unit of a teaching hospital in Nepal...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28428775/genomics-reveals-a-unique-clone-of-burkholderia-cenocepacia-harboring-an-actively-excising-novel-genomic-island
#3
Prashant P Patil, Swapna Mali, Samriti Midha, Vikas Gautam, Lona Dash, Sunil Kumar, Jayanthi Shastri, Lipika Singhal, Prabhu B Patil
Burkholderia cenocepacia is a clinically dominant form among the other virulent species of Burkholderia cepacia complex (Bcc). In the present study, we sequenced and analyzed the genomes of seven nosocomial Bcc isolates, five of which were isolated from the bloodstream infections and two isolates were recovered from the hospital setting during the surveillance. Genome-based species identification of the Bcc isolates using a type strain explicitly identified the species as B. cenocepacia. Moreover, single nucleotide polymorphism analysis revealed that the six isolates were clonal and phylogenetically distinct from the other B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28400528/within-host-evolution-of-burkholderia-pseudomallei-during-chronic-infection-of-seven-australasian-cystic-fibrosis-patients
#4
Linda T Viberg, Derek S Sarovich, Timothy J Kidd, James B Geake, Scott C Bell, Bart J Currie, Erin P Price
Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between ~4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components...
April 11, 2017: MBio
https://www.readbyqxmd.com/read/28364441/survival-after-lung-transplantation-for-cystic-fibrosis-in-sweden%C3%A2
#5
Marita Gilljam, Ulla Nyström, Göran Dellgren, Ingrid Skog, Lennart Hansson
OBJECTIVES: In Sweden, lung transplantation has been performed in patients with end-stage lung disease since 1990. We assessed survival after lung transplantation for cystic fibrosis (CF) with focus on early mortality and outcome for patients infected with certain multiresistant bacteria, considered a relative contraindication for lung transplantation. METHODS: Review of CF and transplant databases and patient charts. The Kaplan-Meier method and log-rank test were used for survival analysis and group comparison...
March 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28364392/burkholderia-cepacia-complex-in-cystic-fibrosis-in-the-post-epidemic-period-multilocus-sequence-typing-based-approach
#6
Libor Fila, Pavel Dřevínek
Cystic fibrosis (CF) patients in the Czech Republic suffered in the late 1990s from an epidemic with ST32 strain of Burkholderia cepacia complex (Bcc). Cohort segregation of Bcc and of ST32 positive patients was introduced in 1999 and 2002, respectively. We performed a study to evaluate the molecular epidemiology of Bcc infection after implementation of these infection control measures. Patients attending the Prague CF adult Centre from 2000 to 2015 were included in the present study. Demographic data and microbial statuses were collected from patient records...
March 31, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28348799/community-acquired-urinary-tract-infections-caused-by-burkholderiacepacia-complex-in-patients-with-no-underlying-risk-factor
#7
Laila Nimri, Mamuno Sulaiman, Osama Bani Hani
Introduction. Urinary tract infections (UTIs) remain common infections diagnosed in outpatients as well as hospitalized patients. Community-acquired UTIs are generally caused by Escherichia coli and other members of the family Enterobacteriaceae.Burkholderiacepacia is an opportunistic pathogen mainly affecting immunocompromised and hospitalized patients, particularly those who have received prior broad-spectrum antibacterial therapy. Case presentation. Urine samples were collected from 157 outpatients clinically diagnosed with UTI and from 100 healthy control subjects...
January 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28270796/volcanic-soils-as-sources-of-novel-co-oxidizing-paraburkholderia-and-burkholderia-paraburkholderia-hiiakae-sp-nov-paraburkholderia-metrosideri-sp-nov-paraburkholderia-paradisi-sp-nov-paraburkholderia-peleae-sp-nov-and-burkholderia-alpina-sp-nov-a-member-of
#8
Carolyn F Weber, Gary M King
Previous studies showed that members of the Burkholderiales were important in the succession of aerobic, molybdenum-dependent CO oxidizing-bacteria on volcanic soils. During these studies, four isolates were obtained from Kilauea Volcano (Hawai'i, USA); one strain was isolated from Pico de Orizaba (Mexico) during a separate study. Based on 16S rRNA gene sequence similarities, the Pico de Orizaba isolate and the isolates from Kilauea Volcano were provisionally assigned to the genera Burkholderia and Paraburkholderia, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28264560/overcoming-an-extremely-drug-resistant-xdr-pathogen-avibactam-restores-susceptibility-to-ceftazidime-for-burkholderia-cepacia-complex-isolates-from-cystic-fibrosis-patients
#9
Krisztina M Papp-Wallace, Scott A Becka, Elise T Zeiser, Nozomi Ohuchi, Maria F Mojica, Julian A Gatta, Monica Falleni, Delfina Tosi, Elisa Borghi, Marisa L Winkler, Brigid M Wilson, John J LiPuma, Michiyoshi Nukaga, Robert A Bonomo
Burkholderia multivorans is a significant health threat to persons with cystic fibrosis (CF). Infections are difficult to treat as this pathogen is inherently resistant to multiple antibiotics. Susceptibility testing of isolates obtained from CF respiratory cultures revealed that single agents selected from different antibiotic classes were unable to inhibit growth. However, all isolates were found to be susceptible to ceftazidime when combined with the novel non-β-lactam β-lactamase inhibitor, avibactam (all minimum inhibitor concentrations (MICs) were ≤8 mg/L of ceftazidime and 4 mg/L of avibactam)...
March 30, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28262569/pilot-trial-of-tobramycin-inhalation-powder-in-cystic-fibrosis-patients-with-chronic-burkholderia-cepacia-complex-infection
#10
Valerie Waters, Yvonne Yau, Trevor Beaudoin, Jillian Wettlaufer, Sean Kevin Tom, Nancy McDonald, Leena Rizvi, Michelle Klingel, Felix Ratjen, Elizabeth Tullis
There is no effective chronic suppressive therapy Burkholderia cepacia complex infection in cystic fibrosis (CF) patients. This was a pilot, open-label clinical trial of tobramycin inhalation powder (TIP) delivered via Podhaler twice daily for 28days in adults and children with CF and chronic B. cepacia complex infection in Toronto, Canada. A total of 10 subjects (4 pediatric, 6 adult patients) were treated. There was a mean drop of 1.4 log (CFU/ml) in sputum bacterial density (p=0.01) and sputum IL-8 levels decreased significantly after 28days of TIP (p=0...
March 2, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28258146/a-broad-host-range-tailocin-from-burkholderia-cenocepacia
#11
Guichun W Yao, Iris Duarte, Tram T Le, Lisa Carmody, John J LiPuma, Ry Young, Carlos F Gonzalez
The Burkholderia cepacia complex (Bcc) consists of 20 closely related gram-negative bacterial species that are significant pathogens for persons with cystic fibrosis (CF). Some Bcc strains are highly transmissible and resistant to multiple antibiotics, making infection difficult to treat. A tailocin (phage-tail-like bacteriocin), designated BceTMilo, with broad host range against members of the Bcc was identified in Burkholderia cenocepacia strain BC0425. Sixty-eight percent of Bcc representing 10 species and 90% of non-Bcc Burkholderia strains tested were sensitive to BceTMilo...
March 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28255573/evaluation-of-burkholderia-cepacia-complex-bacteria-pathogenicity-using-caenorhabditis-elegans
#12
Pietro Tedesco, Elia Di Schiavi, Fortunato Palma Esposito, Donatella de Pascale
This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.
October 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28236043/cystic-fibrosis-in-austria
#13
Thomas Frischer, Ernst Eber, Helmut Ellemunter, Angela Zacharasiewicz, Ingrid Kaluza, Josef Riedler, Sabine Renner
Registry data for patients with cystic fibrosis (CF) are increasingly used to evaluate the natural history, for benchmarking of therapy and in order to identify eligible patients for clinical studies. So far, no data on frequency and clinical status of CF patients have been available for Austria on a national level. We collected data of CF patients treated 2014 in Austrian CF outpatient clinics by means of a European CF registry and on an individual search basis. A total of 773 CF patients with a median age of 18...
February 24, 2017: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/28228494/an-evaluation-of-rgm-medium-for-isolation-of-nontuberculous-mycobacteria-from-respiratory-samples-from-patients-with-cystic-fibrosis-in-the-united-states
#14
Rongpong Plongla, Clair L Preece, John D Perry, Peter H Gilligan
A novel selective agar (RGM medium) has been advocated for the isolation of rapidly growing mycobacteria from the sputum of cystic fibrosis (CF) patients. The aim of this study was to compare RGM medium with Burkholderia cepacia selective agar (BCSA) and a standard AFB culture method for the isolation of nontuberculous mycobacteria (NTM) from patients with CF. The applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of NTM isolated on RGM was also assessed...
February 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28177793/biocontrol-of-burkholderia-cepacia-complex-bacteria-and-bacterial-phytopathogens-by-bdellovibrio-bacteriovorus
#15
Damian McNeely, Ryan M Chanyi, James S Dooley, John E Moore, Susan F Koval
Bdellovibrio and like organisms are predatory bacteria that have the unusual property of using the cytoplasmic constituents of other Gram-negative bacteria as nutrients. These predators may thus provide an alternative approach to the biocontrol of human and plant pathogens. Predators were isolated on Burkholderia cenocepacia K56-2 and J2315 as prey cells, in enrichment cultures with soil and sewage. Three isolates (DM7C, DM8A, and DM11A) were identified as Bdellovibrio bacteriovorus on the basis of morphology, a periplasmic life cycle, and 16S rRNA gene sequencing...
April 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28166854/an-outbreak-of-burkholderia-cepacia-complex-infections-associated-with-contaminated-liquid-docusate
#16
Lucila Marquez, Katie N Jones, Elaine M Whaley, Tjin H Koy, Paula A Revell, Ruston S Taylor, M Brooke Bernhardt, Jeffrey L Wagner, James J Dunn, John J LiPuma, Judith R Campbell
OBJECTIVE To investigate an outbreak of Burkholderia cepacia complex and describe the measures that revealed the source. SETTING A 629-bed, tertiary-care, pediatric hospital in Houston, Texas. PATIENTS Pediatric patients without cystic fibrosis (CF) hospitalized in the pediatric and cardiovascular intensive care units. METHODS We investigated an outbreak of B. cepacia complex from February through July 2016. Isolates were evaluated for molecular relatedness with repetitive extragenic palindromic polymerase chain reaction (rep-PCR); specific species identification and genotyping were performed at an independent laboratory...
February 7, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28164789/preliminary-data-on-antibacterial-activity-of-echinacea-purpurea-associated-bacterial-communities-against-burkholderia-cepacia-complex-strains-opportunistic-pathogens-of-cystic-fibrosis-patients
#17
Carolina Chiellini, Isabel Maida, Valentina Maggini, Emanuele Bosi, Stefano Mocali, Giovanni Emiliani, Elena Perrin, Fabio Firenzuoli, Alessio Mengoni, Renato Fani
Burkholderia cepacia complex bacteria (Bcc) represent a serious threat for immune-compromised patient affected by Cystic Fibrosis (CF) since they are resistant to many substances and to most antibiotics. For this reason, the research of new natural compounds able to inhibit the growth of Bcc strains has raised new interest during the last years. A source of such natural compounds is represented by medicinal plants and, in particular, by bacterial communities associated with these plants able to produce molecules with antimicrobial activity...
March 2017: Microbiological Research
https://www.readbyqxmd.com/read/28120710/the-role-of-universal-stress-proteins-in-bacterial-infections
#18
Andrew O Connor, Siobhán McClean
Universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. A general property of USPs is their role in adaptation of bacteria to oxidative stress, high temperature, low pH and/or hypoxia. There is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. USPs in Mycobacterium tuberculosis are well studied and may play a role in latency of tuberculosis...
January 24, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28106859/burkholderia-cepacia-complex-regulation-of-virulence-gene-expression-a-review
#19
REVIEW
Sílvia A Sousa, Joana R Feliciano, Tiago Pita, Soraia I Guerreiro, Jorge H Leitão
Burkholderia cepacia complex (Bcc) bacteria emerged as opportunistic pathogens in cystic fibrosis and immunocompromised patients. Their eradication is very difficult due to the high level of intrinsic resistance to clinically relevant antibiotics. Bcc bacteria have large and complex genomes, composed of two to four replicons, with variable numbers of insertion sequences. The complexity of Bcc genomes confers a high genomic plasticity to these bacteria, allowing their adaptation and survival to diverse habitats, including the human host...
January 19, 2017: Genes
https://www.readbyqxmd.com/read/28046077/an-oxygen-sensing-two-component-system-in-the-burkholderia-cepacia-complex-regulates-biofilm-intracellular-invasion-and-pathogenicity
#20
Matthew M Schaefers, Tiffany L Liao, Nicole M Boisvert, Damien Roux, Deborah Yoder-Himes, Gregory P Priebe
Burkholderia dolosa is a member of the Burkholderia cepacia complex (BCC), which is a group of bacteria that cause chronic lung infection in patients with cystic fibrosis (CF) and can be associated with outbreaks carrying high morbidity and mortality. While investigating the genomic diversity of B. dolosa strains collected from an outbreak among CF patients, we previously identified fixL as a gene showing signs of strong positive selection. This gene has homology to fixL of the rhizobial FixL/FixJ two-component system...
January 2017: PLoS Pathogens
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