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https://www.readbyqxmd.com/read/28741348/super-resolution-far-field-infrared-imaging-by-photothermal-heterodyne-imaging
#1
Zhongming Li, Kyle Aleshire, Masaru Kuno, Gregory V Hartland
Infrared (IR) imaging provides chemical-specific information without the need for exogenous labels. Conventional far-field IR imaging techniques are diffraction limited, which means an effective spatial resolution of > 5 μm with currently available optics. In this paper we present a novel far-field IR imaging technique based on photothermal heterodyne imaging (IR-PHI). In our version of IR-PHI an IR pump laser excites the sample, causing a small temperature rise that is detected by a counter-propagating visible probe beam...
July 25, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28736786/an-enzyme-free-flow-cytometric-bead-assay-for-the-sensitive-detection-of-micrornas-based-on-click-nucleic-acid-ligation-mediated-signal-amplification
#2
Yan Qi, Liying Qiu, Wenjiao Fan, Chenghui Liu, Zhengping Li
A versatile flow cytometric bead assay (FCBA) coupled with a completely enzyme-free signal amplification mechanism is developed for the sensitive detection of microRNAs (miRNAs). This new strategy integrates click chemistry-mediated ligation chain reaction (CLCR) with hybridization chain reaction (HCR) for enzyme-free signal amplification on magnetic beads (MBs), and a flow cytometer for the robust fluorescence readout of the MBs. Firstly, target miRNA can initiate CLCR on the surface of MBs based on the click chemical ligation between dibenzocyclooctyne (DBCO)- and azide-modified single-stranded DNA (ssDNA) probes, and the amount of ligated ssDNA sequences on the MBs will be proportional to the dosage of target miRNA...
July 24, 2017: Analyst
https://www.readbyqxmd.com/read/28730476/proteomic-profiling-of-protein-kinase-inhibitor-targets-by-mass-spectrometry
#3
Martin Golkowski, Dustin J Maly, Shao-En Ong
Identifying cellular targets of bioactive small molecules from large-scale screening campaigns can be a significant bottleneck in developing novel therapeutics. Our rapid small-molecule target profiling protocol combines affinity enrichment and SILAC for proteomic identification of small molecule-protein interactions. Selective interactions are easily discernable from nonspecific protein binding by quantitative ratios. Using kinase inhibitors as an example, we provide an optimized protocol featuring on-bead protein digestion and single nano-flow liquid chromatographic-mass spectrometric (LC-MS) analyses, consequently increasing analytical throughput and sensitivity over gel-based sample preparation methods for rapid profiling of kinase inhibitor targets...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28719497/serologic-responses-in-childhood-pulmonary-tuberculosis
#4
Bareng A S Nonyane, Mark P Nicol, Nicholas J Andreas, Stefanie Rimmele, Nicole Schneiderhan-Marra, Lesley J Workman, Mark D Perkins, Thomas Joos, Tobias Broger, Jerrold J Ellner, David Alland, Beate Kampmann, Susan E Dorman, Heather J Zar
BACKGROUND: Identification of the Mycobacterium tuberculosis immunoproteome and antigens associated with serologic responses in adults has renewed interest in developing a serologic test for childhood tuberculosis (TB). We investigated IgG antibody responses against M. tuberculosis antigens in children with well-characterized TB. METHODS: We studied archived sera obtained from hospitalized children with suspected pulmonary TB, and classified as having confirmed TB (culture-confirmed), unlikely TB (clinical improvement without TB treatment), or unconfirmed TB (all others)...
July 15, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28718373/screening-of-specific-nucleic-acid-aptamers-binding-tumor-markers-in-the-serum-of-the-lung-cancer-patients-and-identification-of-their-activities
#5
Kun Li, Chen-Lin Xiu, Li-Ming Gao, Hua-Gang Liang, Shu-Feng Xu, Ming Shi, Jian Li, Zhi-Wei Liu
Lung cancer is by far the leading cause of cancer death in the world. Despite the improvements in diagnostic methods, the status of early detection was not achieved. So, a new diagnostic method is needed. The aim of this study is to obtain the highly specific nucleic acid aptamers with strong affinity to tumor markers in the serum of the lung cancer patients for targeting the serum. Aptamers specifically binding to tumor markers in the serum of the lung cancer patients were screened from the random single-stranded DNA library with agarose beads as supports and the serum as a target by target-substituting subtractive SELEX technique and real-time quantitative polymerase chain reaction technique...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28714174/immunological-properties-and-protective-efficacy-of-a-single-mycobacterial-antigen-displayed-on-polyhydroxybutyrate-beads
#6
Patricia Rubio-Reyes, Natalie A Parlane, Bryce M Buddle, D Neil Wedlock, Bernd H A Rehm
In 2015, there were an estimated 10.4 million new tuberculosis (TB) cases and 1.4 million deaths worldwide. Bacille Calmette-Guérin (BCG), an attenuated strain of Mycobacterium bovis, is the vaccine available against TB, but it is insufficient for global TB control. This study evaluated the immunogenicity of the Mycobacterium tuberculosis antigen Rv1626 in mice while assessing the effect of co-delivering either Cpe30 (immunostimulatory peptide), CS.T3378-395 (promiscuous T helper epitope) or flagellin (TLR5 agonist) or a combination of all three immunostimulatory agents...
July 17, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28712383/retrospective-genomic-analysis-of-vibrio-cholerae-o1-el-tor-strains-from-different-places-in-india-reveals-the-presence-of-ctxb-7-allele-found-in-haitian-isolates
#7
R DE, T Ramamurthy, B L Sarkar, A K Mukhopadhyay, G P Pazhani, S Sarkar, S Dutta, G B Nair
A total of 45 strains of Vibrio cholerae O1 isolated from 10 different places in India where they were associated with cases of cholera between the years 2007 and 2008 were examined by molecular methods. With the help of phenotypic and genotypic tests the strains were confirmed to be O1 El Tor biotype strains with classical ctxB gene. Polymerase chain reaction (PCR) analysis by double - mismatch amplification mutation assay PCR showed 16 of these strains carried the ctxB-7 allele reported in Haitian strains...
August 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28709138/multimodal-nonlinear-microscope-based-on-a-compact-fiber-format-laser-source
#8
Francesco Crisafi, Vikas Kumar, Antonio Perri, Marco Marangoni, Giulio Cerullo, Dario Polli
We present a multimodal non-linear optical (NLO) laser-scanning microscope, based on a compact fiber-format excitation laser and integrating coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and two-photon-excitation fluorescence (TPEF) on a single platform. We demonstrate its capabilities in simultaneously acquiring CARS and SRS images of a blend of 6-μm poly(methyl methacrylate) beads and 3-μm polystyrene beads. We then apply it to visualize cell walls and chloroplast of an unprocessed fresh leaf of Elodea aquatic plant via SRS and TPEF modalities, respectively...
July 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28706232/self-organized-micro-spiral-of-single-walled-carbon-nanotubes
#9
Keisuke Mae, Hidetoshi Toyama, Erika Nawa-Okita, Daigo Yamamoto, Yong-Jun Chen, Kenichi Yoshikawa, Fumiyuki Toshimitsu, Naotoshi Nakashima, Kazunari Matsuda, Akihisa Shioi
Single-walled carbon nanotubes (SWCNTs) are reported to spontaneously align in a rotational pattern by drying a liquid droplet of toluene containing polyfluorene as a dispersant. By situating a droplet of an SWCNT solution around a glass bead, spiral patterns are generated. The parallel alignment of SWCNTs along one stripe of such a pattern is confirmed using scanning electron microscopy and polarized optical microscopy. The orientation order increases toward the outer edge of a stripe. The stripe width in the pattern is proportional to the solute concentration, and the width and position of the stripes follow geometric sequences...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705059/comparison-of-triple-drug-transcatheter-arterial-chemoembolization-tace-with-single-drug-tace-using-doxorubicin-eluting-beads-long-term-survival-in-313-patients
#10
Antoinette S Gomes, Phillip A Monteleone, James W Sayre, Richard S Finn, Saeed Sadeghi, Myron J Tong, Carolyn D Britten, Ronald W Busuttil
OBJECTIVE: We compared survival outcomes in 313 patients with unresectable hepatocellular carcinoma (HCC) treated with two different transcatheter arterial chemoembolization (TACE) regimens: triple-drug TACE or single-drug TACE using drug-eluting beads. MATERIALS AND METHODS: In this retrospective study, patient selection criteria were uniform. The triple-drug group (n = 166) underwent TACE using ethiodized oil with doxorubicin, cisplatin, and mitomycin-C with a microsphere embolic...
July 13, 2017: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/28700121/development-of-a-bead-based-multiplexed-assay-for-simultaneous-quantification-of-five-bovine-cytokines-by-flow-cytometry
#11
Valérie Rodrigues, Jean Baptiste Baudier, Isabelle Chantal
Quantifying cytokines is extremely important in studies of host-pathogen interactions. Multiplex assays are commercially available but only for human and mouse cytokines. Here a method for the simultaneous quantification of five important bovine cytokines IFNγ, IL-4, IL-10, IL-12, and TNFα in cell culture supernatants, using flow cytometry was reported. Functional beads from BD Biosciences expressing specific APC intensity were used. Commercially available antibodies against bovine cytokines were covalently coupled to beads as capture antibodies...
July 12, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28692591/development-and-validation-of-a-modular-endoscopic-ear-surgery-skills-trainer
#12
Matthew M Dedmon, Brendan P O'Connell, Elliott D Kozin, Aaron K Remenschneider, Samuel R Barber, Daniel J Lee, Robert F Labadie, Alejandro Rivas
OBJECTIVE: Endoscopic ear surgery (EES) is an emerging technique requiring single-handed dissection with limited depth perception. Current options for EES simulation and training are limited. Herein, we introduce a versatile, low-cost surgical skills trainer that aims to improve the fine motor control necessary for EES. STUDY DESIGN: Prospective validation study. SETTING: Surgical simulation laboratory. PARTICIPANTS: Seven subjects ranging in experience from medical students ("Novices") to experienced ear surgeons ("Experts") participated in the validation study...
July 7, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28689023/profiling-of-cytokines-chemokines-and-other-soluble-proteins-as-a-potential-biomarker-in-colorectal-cancer-and-polyps
#13
Nor Adzimah Johdi, Luqman Mazlan, Ismail Sagap, Rahman Jamal
Soluble proteins including cytokines, chemokines and growth factors are small proteins that mediate and regulate immunity. They involved in the pathogenesis of many diseases including cancers. The concentration of these proteins in biological fluids (serum or plasma) and tissues in diseases may suggest pathway activation that leads to inflammatory response or disease progression. Therefore, these soluble proteins may be useful as a tool for screening, diagnosis classification between stages of disease or surveillance for therapy...
July 6, 2017: Cytokine
https://www.readbyqxmd.com/read/28687035/effect-of-eudragit-s100-nanoparticles-and-alginate-chitosan-encapsulation-on-the-viability-of-lactobacillus-acidophilus-and-lactobacillus-rhamnosus
#14
Fereshteh Ansari, Hadi Pourjafar, Vahid Jodat, Javad Sahebi, Amir Ataei
In this study, we examined a novel method of microencapsulation with calcium alginate-chitosan and Eudragit S100 nanoparticles for the improving viability of probiotic bacteria, Lactobacillus acidophilus and Lactobacillus rhamnosus. Extrusion technique was carried out in microencapsulation process. The viability of two probiotics in single coated beads (with only chitosan), double coated beads (with chitosan and Eudragit nanoparticles), and as free cells (unencapsulated) were conducted in simulated gastric juice (pH 1...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28686280/spectral-reading-of-optical-resonance-encoded-cells-in-microfluidics
#15
Matjaž Humar, Avinash Upadhya, Seok Hyun Yun
The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes...
July 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28685569/point-of-care-assay-of-telomerase-activity-at-single-cell-level-via-gas-pressure-readout
#16
Yanjun Wang, Luzhu Yang, Baoxin Li, Chaoyong James Yang, Yan Jin
Detection of telomerase activity at the single-cell level is one of the central challenges in cancer diagnostics and therapy. Herein, we describe a facile and reliable point-of-care testing (POCT) strategy for detection of telomerase activity via a portable pressure meter. Telomerase primer (TS) was immobilized onto the surface of magnetic beads (MBs), and then was elongated to a long single-stranded DNA by telomerase. The elongated (TTAGGG)n repeat unit hybridized with several short PtNP-functionalized complementary DNA (PtNPs-cDNA), which specifically enriched PtNPs onto the surfaces of magnetic beads (MBs), which were separated using a magnet...
July 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28678845/polymerase-free-measurement-of-microrna-122-with-single-base-specificity-using-single-molecule-arrays-detection-of-drug-induced-liver-injury
#17
David M Rissin, Barbara López-Longarela, Salvatore Pernagallo, Hugh Ilyine, A D Bastiaan Vliegenthart, James W Dear, Juan J Díaz-Mochón, David C Duffy
We have developed a single probe method for detecting microRNA from human serum using single molecule arrays, with sequence specificity down to a single base, and without the use of amplification by polymerases. An abasic peptide nucleic acid (PNA) probe-containing a reactive amine instead of a nucleotide at a specific position in the sequence-for detecting a microRNA was conjugated to superparamagnetic beads. These beads were incubated with a sample containing microRNA, a biotinylated reactive nucleobase-containing an aldehyde group-that was complementary to the missing base in the probe sequence, and a reducing agent...
2017: PloS One
https://www.readbyqxmd.com/read/28674876/bead-based-and-multiplexed-immunoassays-for-protein-profiling-via-sequential-affinity-capture
#18
Elin Birgersson, Jochen M Schwenk, Burcu Ayoglu
Antibody microarrays offer high-throughput immunoassays for multiplexed analyses of clinical samples. For such approaches, samples are either labeled in solution to enable a direct readout on the single binder assay format or detected by matched pairs of capture and detection antibodies in dual binder assay format, also known as sandwich assays. Aiming to benefit from the flexibility and capacity offered by single binder assay readout and the specificity and sensitivity of dual binder assays, we developed a multiplexed dual binder procedure that is based on a sequential, rather than combined, antigen binding...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28671246/genetic-correlation-and-genome-wide-association-study-gwas-of-the-length-of-productive-life-days-open-and-305-days-milk-yield-in-crossbred-holstein-dairy-cattle
#19
P Saowaphak, M Duangjinda, S Plaengkaeo, R Suwannasing, W Boonkum
In this study, we estimated the genetic parameters and identified the putative quantitative trait loci (QTL) associated with the length of productive life (LPL), days open (DO), and 305-day milk yield for the first lactation (FM305) of crossbred Holstein dairy cattle. Data comprising 4,739 records collected between 1986 and 2004 were used to estimate the variance-covariance components using the multiple-trait animal linear mixed models based on the average information restricted maximum likelihood (AI-REML) algorithm...
June 29, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28667615/protein-protein-interactions-co-immunoprecipitation
#20
Jer-Sheng Lin, Erh-Min Lai
Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidence shows that protein-protein interactions are crucial in many biological processes in living cells. Genetic (such as yeast two-hybrid, Y2H) and biochemical (such as co-immunoprecipitation, co-IP) methods are the methods commonly used at the beginning of a study to identify the interacting proteins. Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify molecules that interact with specific proteins...
2017: Methods in Molecular Biology
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