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https://www.readbyqxmd.com/read/27902371/mapping-b-lymphocytes-as-major-reservoirs-of-naturally-occurring-latent-equine-herpesvirus-5-infection
#1
Zelalem Mekuria, Charles El Hage, Nino P Ficorilli, Elizabeth A Washington, James R Gilkerson, Carol Hartley
Equine herpesvirus 5 (EHV5) is a commonly detected gammaherpesvirus which, along with the closely related EHV2, constitute the only two known Percaviruses that infect horses. Apart from detection in horse populations worldwide and the recent publication of the whole genome, there is little known about the biology and pathogenesis of this virus, with many assumptions made by parallels with EHV2. The long-term survival of gammaherpesviruses within infected hosts involves the establishment and maintenance of latency in selected cell and tissues types, particularly lymphocytes...
November 30, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27897109/effect-of-the-modification-of-p24-peptide-antigen-on-dendritic-cell-uptake-and-t-cell-activation
#2
P L Smith, K J Norgate, E Hegarty, N Grageda, E Heelas, M Sommerfelt, T Lange, Arnt-Ove Hovden, B Sørenson, A Dalgleish, M Bodman-Smith
BACKGROUND: Vacc-4x is a candidate therapeutic vaccine consisting of 4 modified peptides based on conserved regions of HIV-1 p24Gag. Vacc4x has been shown to induce long term cellular immunity in immunized infected individuals resulting a reduction in viral load on treatment interruption. OBJECTIVE: Vacc-4x peptides are modified. In this study the effect of modification on uptake of the peptides into PBMC, their subsequent presentation and antigenicity was tested...
November 29, 2016: Current HIV Research
https://www.readbyqxmd.com/read/27859685/cd147-modulates-the-differentiation-of-t-helper-17-cells-in-patients-with-rheumatoid-arthritis
#3
Hui Yang, Jian Wang, Yu Li, Zhen-Jie Yin, Ting-Ting Lv, Ping Zhu, Yan Zhang
The role of CD147 in regulation of rheumatoid arthritis (RA) is not fully elucidated. The aim of this study was to investigate the effect of cell-to-cell contact of activated CD14(+) monocytes with CD4(+) T cells, and the modulatory role of CD147 on T-helper 17 (Th17) cells differentiation in patients with RA. Twenty confirmed active RA patients and twenty normal controls were enrolled. CD4(+) T cells and CD14(+) monocytes were purified by magnetic beads cell sorting. Cells were cultured under different conditions in CD4(+) T cells alone, direct cell-to-cell contact co-culture of CD4(+) and CD14(+) cells, or indirect transwell co-culture of CD4(+) /CD14(+) cells in response to LPS and anti-CD3 stimulation with or without anti-CD147 antibody pretreatments...
November 16, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27799218/tlr7-agonist-gs-9620-is-a-potent-inhibitor-of-acute-hiv-1-infection-in-human-peripheral-blood-mononuclear-cells
#4
Rujuta A Bam, Derek Hansen, Alivelu Irrinki, Andrew Mulato, Gregg S Jones, Joseph Hesselgesser, Christian R Frey, Tomas Cihlar, Stephen R Yant
GS-9620 is a potent and selective oral Toll-like receptor 7 (TLR7) agonist that directly activates plasmacytoid dendritic cells (pDCs). GS-9620 suppressed hepatitis B virus (HBV) in animal models of chronic infection and transiently activated HIV expression ex vivo in latently-infected peripheral blood mononuclear cells (PBMCs) from virally suppressed patients. Currently, GS-9620 is under clinical evaluation for treating chronic HBV infection and for reducing latent reservoirs in virally-suppressed HIV-infected patients...
October 31, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27731508/peripheral-blood-mononuclear-cells-infiltration-downregulates-decidual-faah-activity-in-an-lps-induced-embryo-resorption-model
#5
Manuel Luis Wolfson, Julieta Aisemberg, Fernando Correa, Ana María Franchi
Maternal infections with gram-negative bacteria are associated with miscarriage and are one of the most common complications during pregnancy. Previous studies from our group have shown that lipopolysaccharide (LPS)-activated infiltrating peripheral blood mononuclear cells (PBMC) into decidual tissue plays an important role in the establishment of a local inflammatory process that results in embryo cytotoxicity and early embryo resorption. Moreover, we have also shown that an increased endocannabinoid tone mediates LPS-induced deleterious effects during early pregnancy loss...
October 12, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27727130/recombinant-human-lactoferrin-modulates-human-pbmc-derived-macrophage-responses-to-bcg-and-lps
#6
Shen-An Hwang, Marian L Kruzel, Jeffrey K Actor
Lactoferrin, an iron-binding glycoprotein found in mammalian mucosal secretions and granules of neutrophils, possesses several immune modulatory properties. Published reports indicate that lactoferrin enhances the efficacy of the tuberculosis vaccine, BCG (Bacillus Calmette Guerin), both by increasing macrophage and dendritic cell ability to stimulate receptive T cells and by modulating the inflammatory response. This report is the first to demonstrate the effects of a recombinant human lactoferrin (10 μg/mL) on human PBMC derived CD14(+) and CD16(+) macrophages stimulated with a strong (LPS, 10 ng/mL) or weaker (BCG, MOI 1:1) stimulator of inflammation...
September 28, 2016: Tuberculosis
https://www.readbyqxmd.com/read/27640362/cd14-monocyte-derived-galectin-9-induces-nk-cell-cytotoxicity-in-chronic-hepatitis-c
#7
Akira Nishio, Tomohide Tatsumi, Takatoshi Nawa, Takahiro Suda, Teppei Yoshioka, Yoshiki Onishi, Satoshi Aono, Minoru Shigekawa, Hayato Hikita, Ryotaro Sakamori, Daisuke Okuzaki, Takasuke Fukuhara, Yoshiharu Matsuura, Naoki Hiramatsu, Tetsuo Takehara
: NK cell activation is associated with both liver injury and persistent infection in chronic hepatitis C (CHC); however, the detail mechanism of this activation has not yet been fully elucidated. As Galectin-9 (Gal-9) is reported to be increased in the serum and liver tissue of CHC patients, we investigated the function of Gal-9 in NK cell activation in CHC. First, we evaluated the function of Gal-9 on NK cytotoxicity in vitro. Gal-9 treatment resulted in increased cytotoxicity of naïve NK cells, and the Gal-9-activated NK cells demonstrated cytotoxicity toward hepatoma cells and T cells...
September 19, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/27633294/mammalian-cell-cultures-as-models-for-mycobacterium-tuberculosis-human-immunodeficiency-virus-hiv-interaction-studies-a-review
#8
REVIEW
Walter Chingwaru, Richard H Glashoff, Jerneja Vidmar, Petrina Kapewangolo, Samantha L Sampson
Mycobacterium tuberculosis and human immunodeficiency virus (HIV) co-infections have remained a major public health concern worldwide, particularly in Southern Africa. Yet our understanding of the molecular interactions between the pathogens has remained poor due to lack of suitable preclinical models for such studies. We reviewed the use, this far, of mammalian cell culture models in HIV-MTB interaction studies. Studies have described the use of primary human cell cultures, including (1) monocyte-derived macrophage (MDM) fractions of peripheral blood mononuclear cell (PBMC), alveolar macrophages (AM), (2) cell lines such as the monocyte-derived macrophage cell line (U937), T lymphocyte cell lines (CEMx174, ESAT-6-specific CD4(+) T-cells) and an alveolar epithelial cell line (A549) and (3) special models such as stem cells, three dimensional (3D) or organoid cell models (including a blood-brain barrier cell model) in HIV-MTB interaction studies...
September 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27621106/granulocyte-augmented-chemokine-production-induced-by-type-ii-collagen-containing-immune-complexes-is-mediated-via-tlr4-in-rheumatoid-arthritis-patients
#9
Vivek Anand Manivel, Azita Sohrabian, Johan Rönnelid
Rheumatoid arthritis (RA) patients with early elevations of antibodies against collagen type II (CII) have a distinct acute onset phenotype, associated with cytokine induction by surface-bound anti-CII-containing immune complexes (ICs) and high C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Polymorphonuclear granulocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) are abundant in the vicinity of CII in RA joints, and both PMN and PBMC reactivity against anti-CII IC individually relate to early joint destruction and early elevation of CRP and ESR in RA...
September 13, 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27612806/cultures-and-co-cultures-of-human-blood-mononuclear-cells-and-endothelial-cells-for-the-biocompatibility-assessment-of-surface-modified-aisi-316l-austenitic-stainless-steel
#10
Maria Stio, Maria Martinesi, Cristina Treves, Francesca Borgioli
Samples of AISI 316L austenitic stainless steel were subjected either to grinding and polishing procedure, or to grinding and then low temperature glow-discharge nitriding treatment, or to grinding, nitriding and subsequently coating with collagen-I. Nitrided samples, even if only ground, show a higher corrosion resistance in PBS solution, in comparison with ground and polished AISI 316L. Biocompatibility was evaluated in vitro by incubating the samples with either peripheral blood mononuclear cells (PBMC) or human umbilical vein endothelial cells (HUVEC), tested separately or in co-culture...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27574269/alloreactivity-and-immunosuppressive-properties-of-articular-chondrocytes-from-osteoarthritic-cartilage
#11
S Abe, H Nochi, H Ito
PURPOSE: To determine whether articular chondrocytes derived from osteoarthritic knee joints could evoke alloreactive proliferation of peripheral blood mononuclear cells (PBMC) and inhibit mitogenic activity of polyclonally activated CD4+ major histocompatibility complex (MHC) class II- restricted T cells in vitro. METHODS: Osteoarthritic cartilages of 17 patients aged 61 to 85 years were harvested during total knee arthroplasty. Chondrocytes were cultured for experiments...
August 2016: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/27538988/progesterone-augments-cell-susceptibility-to-hiv-1-and-hiv-1-hsv-2-co-infections
#12
Viswanath Ragupathy, Wang Xue, Ji Tan, Krishnakumar Devadas, Yamei Gao, Indira Hewlett
In human immunodeficiency virus type 1 (HIV-1)-infected women, oral or injectable progesterone containing contraceptive pills may enhance HIV-1 acquisition in vivo, and the mechanism by which this occurs is not fully understood. In developing countries, Herpes simplex virus type-2 (HSV-2) co-infection has been shown to be a risk for increase of HIV-1 acquisition and, if co-infected women use progesterone pills, infections may increase several fold. In this study, we used an in vitro cell culture system to study the effects of progesterone on HIV-1 replication and to explore the molecular mechanism of progesterone effects on infected cells...
October 2016: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/27475298/mesenchymal-stem-cell-and-derived-exosome-as-small-rna-carrier-and-immunomodulator-to-improve-islet-transplantation
#13
Di Wen, Yang Peng, Di Liu, Yossi Weizmann, Ram I Mahato
Human bone marrow mesenchymal stem cells (hBMSCs) and their exosomes can suppress immune reaction and deliver small RNAs. Thus, they may improve islet transplantation by delivering small RNAs for promoting islet function and inhibiting immune rejection. Here, we proposed an hBMSC and its exosome-based therapy to overcome immune rejection and poor islet function, both of which hinder the success of islet transplantation. We found overexpressed siFas and anti-miR-375 in plasmid encoding shFas and anti-miR-375 transfected hBMSC-derived exosomes, which silenced Fas and miR-375 of human islets and improved their viability and function against inflammatory cytokines...
September 28, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27441095/mycobacterium-tuberculosis-induces-expansion-of-foxp3-positive-cd4-t-cells-with-a-regulatory-profile-in-tuberculin-non-sensitized-healthy-subjects-implications-for-effective-immunization-against-tb
#14
Christina S Hirsch, Roxana Rojas, Mianda Wu, Zahra Toossi
OBJECTIVE: Infection by MTB or exposure to MTB constituents is associated with intense microbial stimulation of the immune system, through both antigenic and TLR components, and induction of a milieu that is rich in pro-inflammatory/anti-inflammatory cytokines. Here, we addressed the basis of induced regulatory T-cell (iT-reg) expansion in response to MTB stimulation, in the absence of prior T cell antigen responsiveness. METHODS: PBMC from HIV-1 un-infected TST negative and TST positive control subjects were stimulated by virulent MTB H37Rv lysate (L), a French press preparation of MTB that includes all bacterial components...
June 2016: Journal of Clinical & Cellular Immunology
https://www.readbyqxmd.com/read/27431529/alteration-of-the-immune-status-of-umbilical-cord-mesenchymal-stem-cells-stimulated-by-tlr1-2-agonist-pam3csk
#15
Pingxi Wang, Fanwei Zeng, Lina He, Jin Wang, Tingjiu Zhang, Dong Zhang
Mesenchymal stem cells (MSCs) have been widely used in clinical trials due to their multiple differentiation ability, low immunogenicity and immunosuppressant effects on immune response. However, accumulating evidence has indicated that MSCs may stimulate in vivo immune responses and result in the disappearance of MSCs following engrafting. Toll‑like receptors (TLRs) are important in immune response induction against invaded pathogens, however, the function of TLRs in regulating the immune status of MSCs has been seldom reported...
September 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27425378/collaboration-of-cancer-associated-fibroblasts-and-tumour-associated-macrophages-for-neuroblastoma-development
#16
Okito Hashimoto, Makiko Yoshida, Yu-Ichiro Koma, Tomoko Yanai, Daiichiro Hasegawa, Yoshiyuki Kosaka, Noriyuki Nishimura, Hiroshi Yokozaki
Neuroblastoma is the most common extracranial solid tumour in children and is histologically classified by its Schwannian stromal cells. Although having fewer Schwannian stromal cells is generally associated with more aggressive phenotypes, the exact roles of other stromal cells (mainly macrophages and fibroblasts) are unclear. Here, we examined 41 cases of neuroblastoma using immunohistochemistry for the tumour-associated macrophage (TAM) markers CD68, CD163, and CD204, and a cancer-associated fibroblast (CAF) marker, alpha smooth muscle actin (αSMA)...
October 2016: Journal of Pathology
https://www.readbyqxmd.com/read/27391078/mesenchymal-stem-cells-msc-regulate-activation-of-granulocyte-like-myeloid-derived-suppressor-cells-g-mdsc-in-chronic-myeloid-leukemia-patients
#17
Cesarina Giallongo, Alessandra Romano, Nunziatina Laura Parrinello, Piera La Cava, Maria Violetta Brundo, Vincenzo Bramanti, Fabio Stagno, Paolo Vigneri, Annalisa Chiarenza, Giuseppe Alberto Palumbo, Daniele Tibullo, Francesco Di Raimondo
It is well known that mesenchymal stem cells (MSC) have a role in promotion of tumor growth, survival and drug-resistance in chronic myeloid leukemia (CML). Recent reports indicated that a subpopulation of myeloid cells, defined as granulocyte-like myeloid-derived suppressor cells (G-MDSC) is increased in these patients. So far, the role of MSC in MDSC expansion and activation into the BM microenvironment remains unexplored. To address this question, here we use a specific experimental model in vitro, co-culturing MSC with peripheral blood mononucleated cells (PBMC) from normal individuals, in order to generate MSC-educated G-MDSC...
2016: PloS One
https://www.readbyqxmd.com/read/27338729/interaction-among-activated-lymphocytes-and-mesenchymal-cells-through-podoplanin-is-critical-for-a-high-il-17-secretion
#18
Mélissa Noack, Ndiémé Ndongo-Thiam, Pierre Miossec
BACKGROUND: During chronic inflammation, immune cells, notably Th17 cells, infiltrate the inflammatory site and interact with local mesenchymal cells. Applied to rheumatoid arthritis (RA), the aim is to study the interactions between synoviocytes and peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to high IL-17 secretion with an interest on podoplanin. METHODS: PBMC from healthy donors and RA patients were co-cultured with RA synoviocytes during 48 h, in the presence or not of phytohemagglutinin...
2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27330811/phenotypic-and-functional-testing-of-circulating-regulatory-t-cells-in-advanced-melanoma-patients-treated-with-neoadjuvant-ipilimumab
#19
Janet Retseck, Robert VanderWeele, Hui-Min Lin, Yan Lin, Lisa H Butterfield, Ahmad A Tarhini
BACKGROUND: We have previously investigated neoadjuvant ipilimumab (ipi) for patients with locally/regionally advanced melanoma. That initial assessment of peripheral blood mononuclear cells (PBMC) showed a significant increase in shared tumor associated antigen specific CD4(+) and CD8(+) T cell activation. We also observed a transient increase in circulating T regulatory cells (Treg) with a parallel increase in total CD4(+) T cells, as well as a significant decrease in circulating myeloid derived suppressor cells (MDSC)...
2016: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/27328392/role-of-podoplanin-in-the-high-interleukin-17a-secretion-resulting-from-interactions-between-activated-lymphocytes-and-psoriatic-skin-derived-mesenchymal-cells
#20
M Noack, N'd Ndongo-Thiam, P Miossec
In the context of psoriasis, T helper type 17 (Th17) cells infiltrate the inflammatory site and interact with local mesenchymal cells, including skin fibroblasts. The aim of this work was to study the interactions of skin-derived fibroblasts with peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to a high interleukin (IL)-17 secretion. A co-culture system between PBMC and skin fibroblasts was developed. Healthy and patient PBMC were added to non-lesional or lesional skin fibroblasts at a 5:1 ratio for 48 h in the presence or not of activation with phytohaemagglutinin (PHA)...
October 2016: Clinical and Experimental Immunology
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