keyword
MENU ▼
Read by QxMD icon Read
search

Pbmc co culture

keyword
https://www.readbyqxmd.com/read/28411882/in-vitro-generation-of-interleukin-10-secreting-b-regulatory-cells-from-donor-adipose-tissue-derived-mesenchymal-stem-cells-and-recipient-peripheral-blood-mononuclear-cells-for-potential-cell-therapy
#1
Kunal S Gupte, Aruna V Vanikar, Hargovind L Trivedi, Chetan N Patel, Jignesh V Patel
BACKGROUND: Interleukin-10 secreting B-cells are a major subset of B-regulatory cells (B-regs), commonly recognized as CD19(+)/38(hi)/24(hi)/IL10(+). They carry out immunomodulation by release of specific cytokines and/or cell-to-cell contact. We have generated B-regs in-vitro from donor adipose tissue derived mesenchymal stem cells (AD-MSC) and renal allograft recipient (RAR) peripheral blood mononuclear cells (PBMC) for potential cell therapy. MATERIAL AND METHODS: Mononuclear cells separated by density gradient centrifugation from 50 ml anti-coagulated blood of 15-RAR and respective donors were analysed for baseline B-regs using appropriate antibodies...
February 2017: Biomedical Journal
https://www.readbyqxmd.com/read/28376277/dendritic-cells-from-rheumatoid-arthritis-patient-peripheral-blood-induce-th17-cell-differentiation-via-mir-363-integrin-%C3%AE-v-tgf-%C3%AE-axis
#2
Feng Pan, Haiyan Xiang, Juzhen Yan, Liquan Hong, Lahong Zhang, Yuhua Liu, Xiao Feng, Chengsong Cai
BACKGROUND AND AIM: Dendritic cells (DCs) are critical regulators of immune responses. This study was to observe the effect of DCs from peripheral blood on the differentiation of Th17 in rheumatoid arthritis (RA) patients. METHODS AND RESULTS: Peripheral blood samples were collected from 30 RA patients and 20 healthy controls, respectively. Flow cytometry results showed that in contrast with Treg cells, the proportion of Th17 cells in T cells and the Th17/Treg ratio were both increased in RA patients...
April 4, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28342803/antigen-specific-ctls-to-produce-autologous-cells-product-for-adoptive-cellular-therapy
#3
Sai Liu, Yi Shao, Jie Xu, Na Jiang, Yanchao Dai, Yu Wang, Huanqing Sun, Jianping Sun, Yonghong Zhang
As antiretroviral therapy provides long term viral suppression but no cure, alternative therapies such as adoptive cellular therapy have thus been investigated in the anti-AIDS field. OBJECTIVE: This study sought to establish a HLA-A02 specific CTL cell culture method with comparison of the effects of different cytokines used in CTL cultivation to decide the best cultivation environment. In order to produce CTLs with targeted HLA-A02 restricted antigen specificity for adoptive cellular therapy, we evaluated autologous PBMC cultivation in different cytokine environment to select a better expansion condition to produce qualified CTL production...
March 22, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28331616/ex-vivo-akt-inhibition-promotes-the-generation-of-potent-cd19car-t-cells-for-adoptive-immunotherapy
#4
Ryan Urak, Miriam Walter, Laura Lim, ChingLam W Wong, Lihua E Budde, Sandra Thomas, Stephen J Forman, Xiuli Wang
BACKGROUND: Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. Generating potent CAR T cells is of increasing importance in the field. Studies have demonstrated the importance of the Akt pathway in the regulation of T cell differentiation and memory formation. We now investigate whether inhibition of Akt signaling during ex vivo expansion of CAR T cells can promote the generation of CAR T cells with enhanced antitumor activity following adoptive therapy in a murine leukemia xenograft model...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28261215/tnf-blockade-maintains-an-il-10-phenotype-in-human-effector-cd4-and-cd8-t-cells
#5
Ceri A Roberts, Lucy E Durham, Veerle Fleskens, Hayley G Evans, Leonie S Taams
CD4(+) and CD8(+) effector T cell subpopulations can display regulatory potential characterized by expression of the prototypically anti-inflammatory cytokine IL-10. However, the underlying cellular mechanisms that regulate expression of IL-10 in different T cell subpopulations are not yet fully elucidated. We recently showed that TNF inhibitors (TNFi) promote IL-10 expression in human CD4(+) T cells, including IL-17(+) CD4(+) T cells. Here, we further characterized the regulation of IL-10 expression via blockade of TNF signaling or other cytokine/co-stimulatory pathways, in human T cell subpopulations...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28260094/immunosuppressive-macrophages-induced-by-ido1-promote-the-growth-of-endometrial-stromal-cells-in-endometriosis
#6
Jie Mei, Kai-Kai Chang, Hai-Xiang Sun
It was previously demonstrated that anomalous expression of indoleamine 2,3-dioxygenase-1 (IDO1) in endometrial stromal cells (ESCs) stimulated an inflammatory response that subsequently initiated the activation of immunosuppressive macrophages in endometriosis. The aim of the present study was to clarify the effect of IDO1‑induced macrophages on the growth of ESCs in endometriosis. Normal ESCs, ectopic ESCs and normal ESCs treated with plasmid pEGFP‑N1‑IDO1 or SD11‑IDO1 short hairpin RNA were co‑cultured with peripheral blood‑derived monocyte (PBMC)‑driven macrophages directly for 48 h...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28238206/synergistic-effect-of-ddt-and-its-metabolites-in-lipopolysaccharide-mediated-tnf-%C3%AE-production-is-inhibited-by-progesterone-in-peripheral-blood-mononuclear-cells
#7
Pablo Dominguez-Lopez, Laura Diaz-Cueto, Arturo Aguilar-Rojas, Fabian Arechavaleta-Velasco
Increased TNF-α levels have been associated with adverse pregnancy outcomes. Lipopolysaccharide (LPS), 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) induce TNF-α release in peripheral blood mononuclear cells (PBMC). Conversely, progesterone (P4) inhibits TNF-α secretion. Pregnant women in malaria endemic areas may be co-exposure to these compounds. Thus, this study was to investigate the synergistic effect of LPS and these pesticides in PBMC and to assess P4 influence on this synergy...
February 26, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28235702/design-synthesis-biological-evaluation-and-x-ray-structural-studies-of-potent-human-dihydroorotate-dehydrogenase-inhibitors-based-on-hydroxylated-azole-scaffolds
#8
Stefano Sainas, Agnese C Pippione, Marta Giorgis, Elisa Lupino, Parveen Goyal, Cristina Ramondetti, Barbara Buccinnà, Marco Piccinini, Rodolpho C Braga, Carolina H Andrade, Mikael Andersson, Ann-Christin Moritzer, Rosmarie Friemann, Stefano Mensa, Salam Al-Kadaraghi, Donatella Boschi, Marco L Lolli
A new generation of potent hDHODH inhibitors designed by a scaffold-hopping replacement of the quinolinecarboxylate moiety of brequinar, one of the most potent known hDHODH inhibitors, is presented here. Their general structure is characterized by a biphenyl moiety joined through an amide bridge with an acidic hydroxyazole scaffold (hydroxylated thiadiazole, pyrazole and triazole). Molecular modelling suggested that these structures should adopt a brequinar-like binding mode involving interactions with subsites 1, 2 and 4 of the hDHODH binding site...
March 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28181858/evaluation-of-immunomodulatory-properties-of-feline-mesenchymal-stem-cells
#9
Maciej Parys, John M Kruger, Vilma Yuzbasiyan-Gurkan
Mesenchymal stem cells (MSC) offer a novel approach to treatment of inflammatory disorders in humans and companion animals. Cats spontaneously develop a wide variety of inflammatory disorders and may potentially benefit from MSC-based therapies. Multiple genes are involved in immunomodulation by MSC and interspecies differences between expressions of these genes exist. The goals of the study were to characterize the expression of genes known to be involved in MSC-based immunomodulation and determine the effect of MSC on proliferation of T cells in coculture experiments with peripheral blood mononuclear cells (PBMC)...
March 28, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28100035/stromal-cells-act-as-guardians-for-endothelial-progenitors-by-reducing-their-immunogenicity-after-co-transplantation
#10
Naima Souidi, Meaghan Stolk, Juliane Rudeck, Dirk Strunk, Katharina Schallmoser, Hans-Dieter Volk, Martina Seifert
Regeneration of injured tissues requires effective therapeutic strategies supporting vasculogenesis. The lack of instantly available autologous cell sources and immunogenicity of allogeneic endothelial (progenitor) cells limits clinical progress. Based on the immunosuppressive potency of mesenchymal stem/progenitor cells (MSCs), we investigated whether crosstalk between endothelial colony-forming progenitor cells (ECFCs) and MSCs during vasculogenesis could lower allogeneic T cell responses against ECFCs allowing long-term engraftment in vivo...
May 2017: Stem Cells
https://www.readbyqxmd.com/read/28075442/increased-expression-of-pd%C3%A2-l1-by-the-human-papillomavirus-16-e7-oncoprotein-inhibits-anticancer-immunity
#11
Chaoqi Liu, Jiao Lu, Huiqun Tian, Wei Du, Lin Zhao, Jing Feng, Ding Yuan, Zhiying Li
Cytotoxic T lymphocyte dysfunction is frequently associated with PD‑L1/PD‑1 pathway activation, and is a principal obstacle in cancer therapy. In the present study, the mechanisms underlying the human papillomavirus (HPV)‑induced evasion of cervical cancer cells to the host immune system via the programmed death ligand  1/programmed death 1 (PD‑L1/PD‑1) signaling pathway was investigated. A significant increase in the expression of the HPV16E7 viral protein and PD‑L1 in cervical tissues was observed when compared with normal cervical tissues...
March 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28045905/human-leukocytes-kill-brugia-malayi-microfilariae-independently-of-dna-based-extracellular-trap-release
#12
Ciaran J McCoy, Barbara J Reaves, Steeve Giguère, Ruby Coates, Balázs Rada, Adrian J Wolstenholme
BACKGROUND: Wuchereria bancrofti, Brugia malayi and Brugia timori infect over 100 million people worldwide and are the causative agents of lymphatic filariasis. Some parasite carriers are amicrofilaremic whilst others facilitate mosquito-based disease transmission through blood-circulating microfilariae (Mf). Recent findings, obtained largely from animal model systems, suggest that polymorphonuclear leukocytes (PMNs) contribute to parasitic nematode-directed type 2 immune responses. When exposed to certain pathogens PMNs release extracellular traps (NETs) in the form of chromatin loaded with various antimicrobial molecules and proteases...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28035385/increased-expression-of-pd-l1-by-the-human-papillomavirus-16-e7-oncoprotein-inhibits-anticancer-immunity
#13
Chaoqi Liu, Jiao Lu, Huiqun Tian, Wei Du, Lin Zhao, Jing Feng, Ding Yuan, Zhiying Li
Cytotoxic T lymphocyte dysfunction is frequently associated with PD‑L1/PD‑1 pathway activation, and is a principal obstacle in cancer therapy. In the present study, the mechanisms underlying the human papillomavirus (HPV)‑induced evasion of cervical cancer cells to the host immune system via the programmed death ligand  1/programmed death 1 (PD‑L1/PD‑1) signaling pathway was investigated. A significant increase in the expression of the HPV16E7 viral protein and PD‑L1 in cervical tissues was observed when compared with normal cervical tissues...
December 23, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27974697/surrogate-in-vitro-activation-of-innate-immunity-synergizes-with-interleukin-7-to-unleash-rapid-antigen-driven-outgrowth-of-cd4-and-cd8-human-peripheral-blood-t-cells-naturally-recognizing-muc1-her2-neu-and-other-tumor-associated-antigens
#14
Latha B Pathangey, Dustin B McCurry, Sandra J Gendler, Ana L Dominguez, Jessica E Gorman, Girish Pathangey, Laurie A Mihalik, Yushe Dang, Mary L Disis, Peter A Cohen
Effective adoptive immunotherapy has proved elusive for many types of human cancer, often due to difficulties achieving robust expansion of natural tumor-specific T-cells from peripheral blood. We hypothesized that antigen-driven T-cell expansion might best be triggered in vitro by acute activation of innate immunity to mimic a life-threatening infection. Unfractionated peripheral blood mononuclear cells (PBMC) were subjected to a two-step culture, first synchronizing their exposure to exogenous antigens with aggressive surrogate activation of innate immunity, followed by γ-chain cytokine-modulated T-cell hyperexpansion...
February 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/27930745/the-ebola-interferon-inhibiting-domains-attenuate-and-dysregulate-cell-mediated-immune-responses
#15
Ndongala Michel Lubaki, Patrick Younan, Rodrigo I Santos, Michelle Meyer, Mathieu Iampietro, Richard A Koup, Alexander Bukreyev
Ebola virus (EBOV) infections are characterized by deficient T-lymphocyte responses, T-lymphocyte apoptosis and lymphopenia. We previously showed that disabling of interferon-inhibiting domains (IIDs) in the VP24 and VP35 proteins effectively unblocks maturation of dendritic cells (DCs) and increases the secretion of cytokines and chemokines. Here, we investigated the role of IIDs in adaptive and innate cell-mediated responses using recombinant viruses carrying point mutations, which disabled IIDs in VP24 (EBOV/VP24m), VP35 (EBOV/VP35m) or both (EBOV/VP35m/VP24m)...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27902371/mapping-b-lymphocytes-as-major-reservoirs-of-naturally-occurring-latent-equine-herpesvirus-5-infection
#16
Zelalem H Mekuria, Charles El-Hage, Nino P Ficorilli, Elizabeth A Washington, James R Gilkerson, Carol A Hartley
Equine herpesvirus 5 (EHV5) is a commonly detected gammaherpesvirus, which, along with the closely related EHV2, constitute the only two known percaviruses that infect horses. Apart from detection in horse populations worldwide and the recent publication of the whole genome, there is little known about the biology and pathogenesis of this virus, with many assumptions made by parallels with EHV2. The long-term survival of gammaherpesviruses within infected hosts involves the establishment and maintenance of latency in selected cell and tissues types, particularly lymphocytes...
March 2017: Journal of General Virology
https://www.readbyqxmd.com/read/27897109/effect-of-the-modification-of-p24-peptide-antigen-on-dendritic-cell-uptake-and-t-cell-activation
#17
P L Smith, K J Norgate, E Hegarty, N Grageda, E Heelas, M Sommerfelt, T Lange, Arnt-Ove Hovden, B Sørenson, A Dalgleish, M Bodman-Smith
BACKGROUND: Vacc-4x is a candidate therapeutic vaccine consisting of 4 modified peptides based on conserved regions of HIV-1 p24Gag. Vacc4x has been shown to induce long term cellular immunity in immunized infected individuals resulting a reduction in viral load on treatment interruption. OBJECTIVE: Vacc-4x peptides are modified. In this study the effect of modification on uptake of the peptides into PBMC, their subsequent presentation and antigenicity was tested...
November 29, 2016: Current HIV Research
https://www.readbyqxmd.com/read/27859685/cd147-modulates-the-differentiation-of-t-helper-17-cells-in-patients-with-rheumatoid-arthritis
#18
Hui Yang, Jian Wang, Yu Li, Zhen-Jie Yin, Ting-Ting Lv, Ping Zhu, Yan Zhang
The role of CD147 in regulation of rheumatoid arthritis (RA) is not fully elucidated. The aim of this study was to investigate the effect of cell-to-cell contact of activated CD14(+) monocytes with CD4(+) T cells, and the modulatory role of CD147 on T-helper 17 (Th17) cells differentiation in patients with RA. Twenty confirmed active RA patients and twenty normal controls were enrolled. CD4(+) T cells and CD14(+) monocytes were purified by magnetic beads cell sorting. Cells were cultured under different conditions in CD4(+) T cells alone, direct cell-to-cell contact co-culture of CD4(+) and CD14(+) cells, or indirect transwell co-culture of CD4(+) /CD14(+) cells in response to LPS and anti-CD3 stimulation with or without anti-CD147 antibody pretreatments...
January 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27799218/tlr7-agonist-gs-9620-is-a-potent-inhibitor-of-acute-hiv-1-infection-in-human-peripheral-blood-mononuclear-cells
#19
Rujuta A Bam, Derek Hansen, Alivelu Irrinki, Andrew Mulato, Gregg S Jones, Joseph Hesselgesser, Christian R Frey, Tomas Cihlar, Stephen R Yant
GS-9620 is a potent and selective oral Toll-like receptor 7 (TLR7) agonist that directly activates plasmacytoid dendritic cells (pDCs). GS-9620 suppressed hepatitis B virus (HBV) in animal models of chronic infection and transiently activated HIV expression ex vivo in latently infected peripheral blood mononuclear cells (PBMCs) from virally suppressed patients. Currently, GS-9620 is under clinical evaluation for treating chronic HBV infection and for reducing latent reservoirs in virally suppressed HIV-infected patients...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27731508/peripheral-blood-mononuclear-cells-infiltration-downregulates-decidual-faah-activity-in-an-lps-induced-embryo-resorption-model
#20
Manuel Luis Wolfson, Julieta Aisemberg, Fernando Correa, Ana María Franchi
Maternal infections with gram-negative bacteria are associated with miscarriage and are one of the most common complications during pregnancy. Previous studies from our group have shown that lipopolysaccharide (LPS)-activated infiltrating peripheral blood mononuclear cells (PBMC) into decidual tissue plays an important role in the establishment of a local inflammatory process that results in embryo cytotoxicity and early embryo resorption. Moreover, we have also shown that an increased endocannabinoid tone mediates LPS-induced deleterious effects during early pregnancy loss...
June 2017: Journal of Cellular Physiology
keyword
keyword
66921
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"