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https://www.readbyqxmd.com/read/28926293/the-development-of-a-novel-wound-healing-material-silk-elastin-sponge
#1
Shingo Kawabata, Katsuya Kawai, Satoshi Somamoto, Kazuo Noda, Yoshitaka Matsuura, Yoko Nakamura, Shigehiko Suzuki
Silk-elastin is a recombinant protein polymer with repeating units of silk and elastin blocks. This novel wound healing promoting material has the ability to self-assemble from a liquid to a gel. We have already reported that an aqueous solution of silk-elastin has the potential to accelerate wound healing; however, there are several problems in applying silk-elastin in the clinical setting. To solve these problems, we developed a silk-elastin sponge that is easy to use in the clinical setting. In the present study, we examined whether the wound healing effect of the silk-elastin sponge is equal to the aqueous solution of silk-elastin in vivo...
September 19, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28926236/biofilms-benefiting-plants-exposed-to-zno-and-cuo-nps-studied-with-a-root-mimetic-hollow-fiber-membrane
#2
Michelle Bonebrake, Kaitlyn C Anderson, Jonathan Valiente, Astrid Rosa Jacobson, Joan E McLean, Anne J Anderson, David W Britt
Plants exist with a consortium of microbes that influence plant health, including response to biotic and abiotic stress. While nanoparticle (NP) - plant interactions are increasingly studied, the effect of NPs on the plant microbiome is less researched. Here a root-mimetic hollow fiber membrane (HFM) is presented for generating biofilms of plant-associated microbes nurtured by artificial root exudates (AREs) to correlate exudate composition with biofilm formation and response to NPs. Two microbial isolates from field-grown wheat, a bacillus endophyte and a pseudomonad root surface colonizer, were examined on HFMs fed with AREs varying in N and C composition...
September 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28926193/association-between-cfh-cfb-arms2-serpinf1-vegfr1-and-vegf-polymorphisms-and-anatomical-and-functional-response-to-ranibizumab-treatment-in-neovascular-age-related-macular-degeneration
#3
Estefania Cobos, Sergio Recalde, Jaouad Anter, Maria Hernandez-Sanchez, Carla Barreales, Leticia Olavarrieta, Alicia Valverde, Marta Suarez-Figueroa, Fernando Cruz, Maximino Abraldes, Julian Pérez-Pérez, Patricia Fernández-Robredo, Luis Arias, Alfredo García-Layana
PURPOSE: We sought to determine if specific genetic single nucleotide polymorphisms (SNPs) influence vascular endothelial growth factor inhibition response to ranibizumab in neovascular age-related macular degeneration (AMD). METHODS: A total of 403 Caucasian patients diagnosed with exudative AMD were included. After a three-injection loading phase, a pro re nata regimen was followed. Nine SNPs from six different genes (CFH, CFB, ARMS2, SERPINF1, VEGFR1, VEGF) were genotyped...
September 19, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28925926/resolution-of-treatment-resistant-subretinal-fluid-in-a-patient-with-exudative-age-related-macular-degeneration-following-endophthalmitis
#4
Peter M Kally, Yossi Sidikaro, Colin A McCannel
PURPOSE: This is the first report to the best of the authors' knowledge to show resolution of subretinal fluid and treatment requirement in a case of exudative age-related macular degeneration (eAMD) with persistent fluid despite treatment that resolved following an episode of culture-positive bacterial endophthalmitis. METHODS: A 73-year-old man with history of eAMD of the right eye presented with acute postinjection bacterial endophthalmitis 3 days after injection...
October 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28924439/corneal-neovascularization-with-associated-lipid-keratopathy-in-a-patient-with-obstructive-sleep-apnea-hypopnea-syndrome-using-a-continuous-positive-airway-pressure-machine
#5
Konstantinos Oikonomakis, Myrsini Petrelli, Konstantinos Andreanos, Andreas Mouchtouris, Petros Petrou, Ilias Georgalas, Dimitrios Papaconstantinou, George Kymionis
OBJECTIVE: To report a case of corneal neovascularization with secondary lipid keratopathy in a patient treated with continuous positive airway pressure (CPAP) for obstructive sleep apnea-hypopnea syndrome (OSAHS). CASE REPORT: A 49-year-old male had been diagnosed with obstructive sleep apnea syndrome 10 years ago and has been treated with the application of a CPAP machine during night sleep ever since. For the past year, the patient had been complaining for ocular irritation and excessive tearing of the left eye on awakening...
May 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28923414/a-proteomic-approach-to-the-mechanisms-underlying-activation-of-aluminium-resistance-in-roots-of-urochloa-decumbens
#6
Catalina Arroyave, Roser Tolrà, Livia Chaves, Marcelo Claro de Souza, Juan Barceló, Charlotte Poschenrieder
The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72-96h and is preceded by a sensitive phase (24-48h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96h exposure to 0 or 200μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase...
September 10, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28923303/an-extremely-high-bioavailability-of-orally-administered-vancomycin-in-a-patient-with-severe-colitis-and-renal-insufficiency
#7
Shingo Yamazaki, Takaaki Suzuki, Tatsuya Suzuki, Hirokazu Takatsuka, Masayuki Ishikawa, Noriyuki Hattori, Takeshi Fujishiro, Hideaki Miyauchi, Takehiko Oami, Noritaka Ariyoshi, Shigeto Oda, Hisahiro Matsubara, Itsuko Ishii
Because there is little absorption of orally administered vancomycin hydrochloride (VCM) through the normal intestinal microvillus membrane, the pharmacokinetics of VCM absorbed from the digestive tract are mostly unknown. Here we report a case of severe colitis and renal insufficiency in which the serum concentration of VCM reached the supratherapeutic range after oral administration. A 54-year-old man receiving outpatient chemotherapy for rectal cancer was admitted to our hospital for severe sepsis and acute renal failure...
September 8, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28919726/gene-expression-levels-of-the-insulin-like-growth-factor-family-in-patients-with-amd-before-and-after-ranibizumab-intravitreal-injections
#8
Barbara Strzalka-Mrozik, Malgorzata Kimsa-Furdzik, Adam Kabiesz, Katarzyna Michalska-Malecka, Malgorzata Nita, Urszula Mazurek
PURPOSE: The present study focused on the assessment of the mRNA levels of the insulin-like growth factor (IGF) family in patients with the exudative form of age-related macular degeneration (AMD) before and after ranibizumab intravitreal injections. PATIENTS AND METHODS: An analysis of the expression profile of the IGF family of genes in patients with AMD was carried out using the oligonucleotide microarray and quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) methods...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/28918656/diagnosing-kingella-kingae-infections-in-infants-and-young-children
#9
Pablo Yagupsky
Kingella kingae is currently recognized as the prime etiology of skeletal system infections in children aged 6-48 months. The organism is notoriously fastidious, its growth is inhibited by synovial fluid and bone exudates, and its presence in clinical specimens is commonly missed by traditional culture methods. Areas covered. The present review discusses the use of improved laboratory methods to detect the organism in normally sterile body fluids, exudates, and upper respiratory tract specimens. Expert commentary...
September 18, 2017: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/28918590/the-role-of-diet-in-shaping-the-chemical-signal-design-of-lacertid-lizards
#10
Simon Baeckens, Roberto García-Roa, José Martín, Raoul Van Damme
Lizards communicate with others via chemical signals, the composition of which may vary among species. Although the selective pressures and constraints affecting chemical signal diversity at the species level remain poorly understood, the possible role of diet has been largely neglected. The chemical signals of many lizards originate from the femoral glands that exude a mixture of semiochemicals, and may be used in a variety of contexts. We analyzed the lipophilic fraction of the glandular secretions of 45 species of lacertid lizard species by gas chromatography/mass spectrometry...
September 16, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28918250/associations-of-cholesteryl-ester-transfer-protein-cetp-gene-variants-with-predisposition-to-age-related-macular-degeneration
#11
Rasa Liutkeviciene, Alvita Vilkeviciute, Greta Streleckiene, Loresa Kriauciuniene, Romanas Chaleckis, Vytenis Pranas Deltuva
PURPOSE: To determine the frequency of the genotypes of single nucleotide polymorphisms (SNPs) in the gene encoding cholesteryl ester transfer protein (CETP) and their associations with age-related macular degeneration (AMD) in the Lithuanian population. STUDY DESIGN: A total of 1264 subjects were examined: 251 patients with early AMD, 206 patients with exudative AMD, and 807 healthy controls. METHODS: The genotyping of CETP (rs5882, rs708272, rs3764261, rs1800775, rs2303790) was carried out using the RT-PCR...
September 13, 2017: Gene
https://www.readbyqxmd.com/read/28917718/olfactory-toxicity-in-rats-following-manganese-chloride-nasal-instillation-a-pilot-study
#12
Melanie L Foster, Deepa B Rao, Taylor Francher, Samantha Traver, David C Dorman
Following inhalation, manganese travels along the olfactory nerve from the olfactory epithelium (OE) to the olfactory bulb (OB). Occupational exposure to inhaled manganese is associated with changes in olfactory function. This pilot study evaluated two related hypotheses: (a) intranasal manganese administration increases OE and OB manganese concentrations; and (b) intranasal manganese exposure impairs performance of previously trained rats on a go-no-go olfactory discrimination (OD) task. Male Fischer 344 rats were trained to either lever press ("go") in response to a positive conditioned stimulus (CS+: vanillin) or to do nothing ("no go") when a negative conditioned stimulus (CS-: amyl acetate) was present...
September 13, 2017: Neurotoxicology
https://www.readbyqxmd.com/read/28914005/delayed-absorption-of-subretinal-fluid-after-retinal-reattachment-surgery-and-associated-choroidal-features
#13
Jong Min Kim, Eun Jung Lee, Ga Eun Cho, Kunho Bae, Ju Yeun Lee, Gyule Han, Se Woong Kang
PURPOSE: The aim of this study was to investigate the incidence and associated clinical factors of delayed absorption of subretinal fluid (SRF) after surgery for rhegmatogenous retinal detachment. METHODS: This study involved 36 eyes of 36 consecutive patients who underwent successful surgery for rhegmatogenous retinal detachment. A complete ophthalmologic evaluation, including clinical fundus examination, optical coherence tomography, and indocyanine green angiography, was conducted before and after surgery...
September 11, 2017: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/28912796/a-low-cost-imaging-method-for-the-temporal-and-spatial-colorimetric-detection-of-free-amines-on-maize-root-surfaces
#14
Truc H Doan, Tu A Doan, Michael J Kangas, AdreAnna E Ernest, Danny Tran, Christina L Wilson, Andrea E Holmes, Erin L Doyle, Tessa L Durham Brooks
Plant root exudates are important mediators in the interactions that occur between plants and microorganisms in the soil, yet much remains to be learned about spatial and temporal variation in their production. This work outlines a method utilizing a novel colorimetric paper to detect spatial and temporal changes in the production of nitrogen-containing compounds on the root surface. While existing methods have made it possible to conduct detailed analysis of root exudate composition, relatively less is known about where in the root system exudates are produced and how this localization changes as the root grows...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28902341/bilateral-retinal-detachments-after-intravitreal-injection-of-adipose-derived-stem-cells-in-a-patient-with-exudative-macular-degeneration
#15
Steven S Saraf, Matthew A Cunningham, Ajay E Kuriyan, Sarah P Read, Philip J Rosenfeld, Harry W Flynn, Thomas A Albini
A 77-year-old woman with exudative macular degeneration underwent bilateral intravitreal injections of "stem cells" at a clinic in Georgia. One month and 3 months after injection, she developed retinal detachments in the left and right eyes, respectively. Increased awareness within the medical community of such poor outcomes is critical so that clinics offering untested practices that have been shown to be potentially harmful to patients can be identified and brought under U.S. Food and Drug Administration oversight...
September 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28899681/comparative-degradomics-of-porcine-and-human-wound-exudates-unravels-biomarker-candidates-for-assessment-of-wound-healing-progression-in-trauma-patients
#16
Fabio Sabino, Fabian E Egli, Simonas Savickas, Jörg Holstein, Daniela Kaspar, Mika Rollmann, Jayachandran N Kizhakkedathu, Tim Pohlemann, Hans Smola, Ulrich Auf dem Keller
Impaired cutaneous wound healing is a major complication in elderly people and patients suffering from diabetes with raising rates in industrialized countries. Heterogeneity of clinical manifestations hampers effective molecular diagnostics and decisions for appropriate therapeutic regimens. Using a customized positional quantitative proteomics workflow, we have established a time-resolved proteome and N-terminome resource from wound exudates in a clinically relevant pig wound model that we exploited as robust template to interpret a heterogeneous dataset from patients undergoing the same wound treatment...
September 9, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28899096/physical-and-rheological-properties-of-a-novel-emulsifier-frost-grape-polysaccharide-fgp-from-vitis-riparia-michx
#17
William T Hay, Steven F Vaughn, Jeffrey A Byars, Gordon W Selling, Derek M Holthaus, Neil P J Price
A novel emulsifier, Frost grape polysaccharide (FGP), isolated from natural exudate of the species Vitis riparia Michx, was physically and rheologically characterized. The determination of the physical, structural, thermodynamic, emulsification and rheological properties of FGP provide essential details for the commercial adoption of this novel plant polysaccharide. FGP is capable of producing exceptionally stable emulsions when compared with the industrially ubiquitous gum arabic (GA). The FGP isolate contained a negligible amount of nitrogen (0...
September 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28898955/aging-of-microplastics-promotes-their-ingestion-by-marine-zooplankton
#18
Renske J E Vroom, Albert A Koelmans, Ellen Besseling, Claudia Halsband
Microplastics (<5 mm) are ubiquitous in the marine environment and are ingested by zooplankton with possible negative effects on survival, feeding, and fecundity. The majority of laboratory studies has used new and pristine microplastics to test their impacts, while aging processes such as weathering and biofouling alter the characteristics of plastic particles in the marine environment. We investigated zooplankton ingestion of polystyrene beads (15 and 30 μm) and fragments (≤30 μm), and tested the hypothesis that microplastics previously exposed to marine conditions (aged) are ingested at higher rates than pristine microplastics...
September 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28898873/two-year-outcomes-of-a-treat-and-extend-regimen-using-intravitreal-aflibercept-injections-for-typical-age-related-macular-degeneration
#19
Arisa Ito, Hidetaka Matsumoto, Masahiro Morimoto, Kensuke Mimura, Hideo Akiyama
PURPOSE: The aim of this study was to evaluate the efficacy of a treat-and-extend (TAE) regimen using intravitreal injection of aflibercept (IVA) for typical age-related macular degeneration (tAMD). METHODS: We retrospectively studied 61 treatment-naïve eyes with tAMD. Best-corrected visual acuity (BCVA), central macular thickness (CMT), central choroidal thickness (CCT), number of injections, and complications during 2 years were evaluated. RESULTS: BCVA significantly improved by on average 0...
September 13, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28895945/excess-labile-carbon-promotes-the-expression-of-virulence-factors-in-coral-reef-bacterioplankton
#20
Anny Cárdenas, Matthew J Neave, Mohamed Fauzi Haroon, Claudia Pogoreutz, Nils Rädecker, Christian Wild, Astrid Gärdes, Christian R Voolstra
Coastal pollution and algal cover are increasing on many coral reefs, resulting in higher dissolved organic carbon (DOC) concentrations. High DOC concentrations strongly affect microbial activity in reef waters and select for copiotrophic, often potentially virulent microbial populations. High DOC concentrations on coral reefs are also hypothesized to be a determinant for switching microbial lifestyles from commensal to pathogenic, thereby contributing to coral reef degradation, but evidence is missing. In this study, we conducted ex situ incubations to assess gene expression of planktonic microbial populations under elevated concentrations of naturally abundant monosaccharides (glucose, galactose, mannose, and xylose) in algal exudates and sewage inflows...
September 12, 2017: ISME Journal
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