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https://www.readbyqxmd.com/read/28651420/-effects-of-hypoxia-inducible-factor-1%C3%AE-on-p311-and-its-influence-on-the-migration-of-murine-epidermal-stem-cells
#1
Z D Xu, H S Li, S Wang, W F He, J Wu, G X Luo
Objective: To explore the effects of hypoxia inducible factor-1α (HIF-1α) on P311 and its influence on the migration of murine epidermal stem cells (ESCs) under hypoxia in vitro. Methods: Two kinds of murine ESCs were isolated and obtained from 15 neonatal wild-type C57BL/6J mice and 5 congeneric source P311 gene knock-out mice, respectively. The first passage of cells were used in the following experiments after morphologic observation and detection of expression of cell surface markers CD71 and CD49f with flow cytometer...
May 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28651260/the-cdd-system-in-computed-tomographic-diagnosis-of%C3%A2-diverticular-disease
#2
Daniel Pustelnik, Fabian Henry Jürgen Elsholtz, Christian Bojarski, Bernd Hamm, Stefan Markus Niehues
Purpose This overview sums up the Classification of Diverticular Disease (CDD) with regard to its application in computed tomographic diagnosis and briefly recapitulates its targeted advantages over preliminary systems. Primarily, application of the CDD in computed tomography diagnostics is described. Differences with respect to the categories of the older systems are pointed out on the level of each CDD type using imaging examples. Materials and Methods The presented images are derived from our institute according to the S2k criteria...
June 26, 2017: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/28651180/does-a-short-term-exposure-to-atrazine-provoke-cellular-senescence-in-chlamydomonas-reinhardtii
#3
Marta Esperanza, Magali Houde, Marta Seoane, Ángeles Cid, Carmen Rioboo
The aim of this study was to investigate the impacts and modes of action of a chemical and nutrient deprivation on the cellular senescence process of Chlamydomonas reinhardtii. Several molecular and cellular parameters related to senescence phenomena were monitored in C. reinhardtii cells exposed for 24h to a sublethal concentration (0.25μM) of the herbicide atrazine and in unexposed 96h cells in an early stationary phase of growth. All endpoints showed the same pattern of response between treatments, except for the intracellular level of calcium, where a significant increase was observed in 24h-exposed cultures compared to 24h-log controls...
June 19, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28650173/polymer-pen-lithography-with-lipids-for-large-area-gradient-patterns
#4
Ravi Kumar, Ainhoa Urtizberea, Souvik Ghosh, Uwe Bog, Quinn Rainer, Steven Lenhert, Harald Fuchs, Michael Hirtz
For many applications in the biomedical sector (especially for cell screening assays, guidance and migration experiments), gradient patterns comprising bioactive compounds over comparably (in regard to a cell size) large areas are key. Polymer pen lithography (PPL), as an inherent highly parallel and large area technique has a great potential to serve in the fabrication of such patterns. We present strategies for the printing of functional phospholipid patterns via PPL that provide tunable feature size and feature density gradients over surface areas of several square millimeters...
June 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28649491/ventricular-shape-and-relative-position-abnormalities-in-preterm-neonates
#5
N Paquette, J Shi, Y Wang, Y Lao, R Ceschin, M D Nelson, A Panigrahy, N Lepore
Recent neuroimaging findings have highlighted the impact of premature birth on subcortical development and morphological changes in the deep grey nuclei and ventricular system. To help characterize subcortical microstructural changes in preterm neonates, we recently implemented a multivariate tensor-based method (mTBM). This method allows to precisely measure local surface deformation of brain structures in infants. Here, we investigated ventricular abnormalities and their spatial relationships with surrounding subcortical structures in preterm neonates...
2017: NeuroImage: Clinical
https://www.readbyqxmd.com/read/28649475/solid-pseudopapillary-tumor-of-the-pancreas-an-unusual-cause-of-abdominal-pain
#6
Talal El Imad, Fady G Haddad, Mayurathan Kesavan, Liliane Deeb, Sherif Andrawes
Solid-pseudopapillary neoplasm (SPN) of the pancreas is a rare tumor that accounts for less than one percent of pancreatic tumors. The diagnosis could be challenging as SPN tend to manifest with nonspecific abdominal symptoms, variable radiological features, and inconsistent morphology. The cellular origin of SPN is unclear and might involve ductal, acinar and endocrine stem cells.  We report a rare case of a 27-year-old female who presented with intermittent abdominal pain for two years, associated with a decrease in appetite...
May 16, 2017: Curēus
https://www.readbyqxmd.com/read/28649031/noise-detection-on-ecg-based-on-agglomerative-clustering-of-morphological-features
#7
João Rodrigues, David Belo, Hugo Gamboa
Biosignals are usually contaminated with artifacts from limb movements, muscular contraction or electrical interference. Many algorithms of the literature, such as threshold methods and adaptive filters, focus on detecting these noisy patterns. This study introduces a novel method for noise and artifact detection in electrocardiogram based on time series clustering. The algorithm starts with the extraction of features that best characterize the shape and behaviour of the signal over time and groups its samples in separated clusters by means of an agglomerative clustering approach...
June 15, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28648946/end-of-therapy-minimal-residual-disease-mrd-measurement-in-children-with-all-does-not-predict-relapse
#8
Tahani Hani Sarrawi, Ismael Zayyat, Fareed Barakat, Maha Rezeq, Salam Abu Jmaian, Faris Madanat
Minimal residual disease (MRD) monitoring opened a new era for childhood acute lymphoblastic leukemia (ALL), and is widely used for risk adapted therapy in the major study groups (Borowitz et al. 2015; Eckert et al., 2015; Peregud-Pogorzelsk et al., 2003). MRD is measured either by polymerase chain reaction (PCR) or flow cytometry (FCM) (Campana, 2009). Most studies have concentrated on measuring MRD during or at end of induction, and after consolidation therapy, to stratify patients and to predict relapse (Sutton et al...
June 17, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28648941/low-grade-fibromyxoid-sarcoma-clinical-morphologic-and-genetic-features
#9
REVIEW
Mustafa Mohamed, Cyril Fisher, Khin Thway
Low-grade fibromyxoid sarcoma (LGFMS) is a bland spindle cell neoplasm that typically arises in the deep soft tissues of the proximal extremities or trunk of young adults. The majority of LGFMS are characterized by a recurrent (7;16)(q34;p11) translocation, resulting in the FUS-CREB3L2 fusion gene, which generates a chimeric protein with transcriptional regulatory activity. Small numbers harbor a FUS-CREB3L1 fusion resulting from t(11;16)(p11;p11), whilst rare cases harbor the EWSR1-CREB3L1 fusion. LGFMS is of low to moderate cellularity and consists of bland spindle cells with small, angulated nuclei and scant, wispy cytoplasm, arranged in a whorled growth pattern and typically showing abrupt transition from myxoid to fibrous areas...
June 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/28648740/patellofemoral-morphology-and-alignment-reference-values-and-dose-response-patterns-for-the-relation-to-mri-features-of-patellofemoral-osteoarthritis
#10
Erin M Macri, David T Felson, Yuqing Zhang, Ali Guermazi, Frank W Roemer, Kay M Crossley, Karim M Khan, Josh J Stefanik
OBJECTIVE: We aimed to (i) determine reference values for trochlear morphology and patellofemoral (PF) alignment in adults without MRI-defined PF full thickness cartilage damage or knee pain; and (ii) evaluate dose-response patterns for these measures with prevalent MRI-defined PF structural damage and/or knee pain. DESIGN: The Framingham Community Cohort is a population-based sample of ambulatory adults aged ≥50 years. We evaluated six morphology and alignment measures using MRI (n=985), and reported reference values (mean ± 2SD) in a subsample without MRI-defined PF full thickness cartilage damage or knee pain (n=563)...
June 22, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28648728/microstructure-characterization-of-hydrothermally-synthesized-pani-v2o5%C3%A2-nh2o-heterojunction-photocatalyst-for-visible-light-induced-photodegradation-of-organic-pollutants-and-non-absorbing-colorless-molecules
#11
Samapti Kundu, Biswarup Satpati, Tanusree Kar, Swapan Kumar Pradhan
Polyaniline intercalated vanadium oxide xerogel (PV) hybrid nanocomposite (NC) with novel structure has been successfully synthesized through a simple hydrothermal route. Detailed structure and microstructure characterization of PV NC is illustrated through Rietveld refinement of XRD data and FESEM/HRTEM images. Both FTIR and XPS spectra confirm the presence of PANI and water molecules within the NC and partial reduction of V(5+) to V(4+), corroborating with the results of XRD pattern analysis. Core-shell structure of PV NC, where PANI sheath layer acts as a shell covering around V2O5·nH2O crystalline core is confirmed from HRTEM images...
June 17, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28648364/differentiation-of-human-pluripotent-stem-cells-into-colonic-organoids-via-transient-activation-of-bmp-signaling
#12
Jorge O Múnera, Nambirajan Sundaram, Scott A Rankin, David Hill, Carey Watson, Maxime Mahe, Jefferson E Vallance, Noah F Shroyer, Katie L Sinagoga, Adrian Zarzoso-Lacoste, Jonathan R Hudson, Jonathan C Howell, Praneet Chatuvedi, Jason R Spence, John M Shannon, Aaron M Zorn, Michael A Helmrath, James M Wells
Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. Distal gut tissues such as cecum and colon, however, have proved considerably more challenging to derive in vitro. Here we report the differentiation of human colonic organoids (HCOs) from hPSCs. We found that BMP signaling is required to establish a posterior SATB2+ domain in developing and postnatal intestinal epithelium. Brief activation of BMP signaling is sufficient to activate a posterior HOX code and direct hPSC-derived gut tube cultures into HCOs...
June 20, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28647772/diseases-of-the-esophagus-a-pattern-approach
#13
REVIEW
Marc S Levine, Stephen E Rubesin
The esophagus may be involved by a variety of morphologic abnormalities, including nodules and plaques, ulceration, distal esophageal strictures and rings, upper or midesophageal strictures, and diffuse narrowing (also known as a small-caliber esophagus). The use of a pattern approach for evaluating esophageal disease on barium studies facilitates diagnosis of a host of pathologic conditions associated with these morphologic abnormalities. This article therefore presents an approach for diagnosing esophageal disease on barium studies that emphasizes the radiographic and clinical features used to differentiate the underlying causes of disease and the diagnostic pitfalls associated with performing and interpreting these studies...
June 24, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28646909/the-morphological-regeneration-and-functional-restoration-of-bladder-defects-by-a-novel-scaffold-and-adipose-derived-stem-cells-in-a-rat-augmentation-model
#14
Qiong Wang, Dong-Dong Xiao, Hao Yan, Yang Zhao, Shi Fu, Juan Zhou, Zhong Wang, Zhe Zhou, Ming Zhang, Mu-Jun Lu
BACKGROUND: Due to the multilineage differentiation ability and paracrine role of adipose-derived stem cells (ASCs) for bladder defect repair, various scaffolds have been applied in combination with ASCs to promote bladder regeneration and restore bladder function. However, the low survival rate of ASCs and the difficulty of promoting bladder functional recovery are still unsolved. To explore these problems, we investigated the feasibility of a novel scaffold seeded with ASCs in a rat model of bladder augmentation...
June 24, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28646473/detection-of-foxo1-break-apart-status-by-fluorescence-in-situ-hybridization-in-atypical-alveolar-rhabdomyosarcoma
#15
Libing Fu, Yaqiong Jin, Chao Jia, Jie Zhang, Jun Tai, Hongbin Li, Feng Chen, Jin Shi, Yongli Guo, Xin Ni, Lejian He
The morphologies of alveolar rhabdomyosarcoma (ARMS) are various. Some cases entirely lack an alveolar pattern and instead display a histological pattern that overlaps with embryonal rhabdomyosarcoma (ERMS). The method of pathological diagnosis of ARMS and ERMS has been updated in the 4th edition of the World Health Organization's guidelines for classification of skeletal muscle tumors. Under the new guidelines, there is still no molecular test to distinguish between these two subtypes of rhabdomyosarcoma (RMS)...
June 16, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28645895/multiparity-improves-outcomes-after-cerebral-ischemia-in-female-mice-despite-features-of-increased-metabovascular-risk
#16
Rodney M Ritzel, Anita R Patel, Monica Spychala, Rajkumar Verma, Joshua Crapser, Edward C Koellhoffer, Anna Schrecengost, Evan R Jellison, Liang Zhu, Venugopal Reddy Venna, Louise D McCullough
Females show a varying degree of ischemic sensitivity throughout their lifespan, which is not fully explained by hormonal or genetic factors. Epidemiological data suggest that sex-specific life experiences such as pregnancy increase stroke risk. This work evaluated the role of parity on stroke outcome. Age-matched virgin (i.e., nulliparous) and multiparous mice were subjected to 60 min of reversible middle cerebral artery occlusion and evaluated for infarct volume, behavioral recovery, and inflammation. Using an established mating paradigm, fetal microchimeric cells present in maternal mice were also tracked after parturition and stroke...
June 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28645847/cardiovascular-morphometry-with-high-resolution-3d-magnetic-resonance-first-application-to-left-ventricle-diastolic-dysfunction
#17
Diego Gallo, Orestis Vardoulis, Pierre Monney, Davide Piccini, Panagiotis Antiochos, Juerg Schwitter, Nikolaos Stergiopulos, Umberto Morbiducci
In this study, an image-based morphometry toolset quantifying geometric descriptors of the left ventricle, aorta and their coupling is applied to investigate whether morphological information can differentiate between subjects affected by diastolic dysfunction (patient group) and their age-matched controls (control group). The ventriculo-aortic region of 20 total participants (10 per group) were segmented from high-resolution 3D magnetic resonance images, from the left ventricle to the descending aorta. Each geometry was divided into segments in correspondence of anatomical landmarks...
June 21, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28645676/a-journey-through-growth-plates-tracking-differences-in-morphology-and-regulation-between-the-spine-and-long-bones-in-a-pig-model
#18
Adalbert Raimann, Alireza Javanmardi, Monika Egerbacher, Gabriele Haeusler
BACKGROUND CONTEXT: The process of linear growth is driven by axial elongation of both long bones and vertebral bodies and accomplished by enchondral ossification. Differences in regulation between the two skeletal sites are mirrored clinically by the age course in body proportions. Whereas growth plates in long bones can easily be discriminated, vertebral growth plates are part of the cartilaginous endplate, which typically shows important species differences. PURPOSE: To describe and compare histological, histomorphometric and regulatory characteristics in the growth plates of the spine and long bones in a porcine model...
June 20, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28645508/merkel-cell-carcinoma-melanoma-metastatic-mimics-of-breast-cancer
#19
REVIEW
Megan L Troxell
Merkel cell carcinoma and melanoma can each occur primarily in breast skin, or metastasize to the breast. The breast is a rare site of metastasis of essentially any and every type of tumor, including carcinomas, sarcomas, and hematolymphoid neoplasms, and 10-30% of breast metastases may represent the initial presentation of disease. Although metastases generally recapitulate histologic features of the primary tumor, they are diagnostically challenging given their rarity and morphologic overlap with breast carcinoma, including special types of breast cancer...
May 31, 2017: Seminars in Diagnostic Pathology
https://www.readbyqxmd.com/read/28643449/morphology-of-the-tongue-of-vermilingua-xenarthra-pilosa-and-evolutionary-considerations
#20
Daniel M Casali, Elisângela Martins-Santos, André L Q Santos, Flávia R Miranda, Germán A B Mahecha, Fernando A Perini
The tongue of anteaters (Xenarthra, Pilosa, Vermilingua) is a highly specialized for myrmecophagy. Here, we describe the topography and histology of the tongue, and compare it to that of other xenarthrans and other myrmecophagous eutherian mammals. The tongue of Vermilingua is long and slender, with an apical protuberance, which differs between Myrmecophagidae and Cyclopes didactylus. In the former, the rostral region is conical, and in the latter, it is dorsoventrally compressed, as observed in sloths. The tongue of Vermilingua has filiform and circumvallate papillae on the surface; foliate and fungiform papillae are absent...
June 22, 2017: Journal of Morphology
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