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https://www.readbyqxmd.com/read/28330302/production-of-bioherbicide-by-phoma-sp-in-a-stirred-tank-bioreactor
#1
Thiarles Brun, Jéssica E Rabuske, Izelmar Todero, Thiago C Almeida, Jair J D Junior, Gustavo Ariotti, Tássia Confortin, Jonas A Arnemann, Raquel C Kuhn, Jerson V C Guedes, Marcio A Mazutti
The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330200/use-of-ca-alginate-immobilized-pseudomonas-aeruginosa-for-repeated-batch-and-continuous-degradation-of-endosulfan
#2
Vijayalakshmi Pradeep, Usha Malavalli Subbaiah
The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was carried out with an objective of reducing the toxicity of Endosulfan by degrading it to less toxic metabolites. The long-term stability of Endosulfan degradation was studied during its repeated batch degradation, carried out over a period of 35 days...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330182/efficient-single-step-chromatographic-purification-of-recombinant-human-antithrombin-rhat-from-saccharomyces-cerevisiae
#3
Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda
Antithrombin (AT) is a glycoprotein that inactivates the several physiological target enzymes of coagulation system. The effect of purification strategies plays a crucial role in getting maximum recovery of yield, purity and biological activity of recombinant human antithrombin (rhAT). In the present work, the task of purifying rhAT from Saccharomyces cerevisiae BY4741 has been carried out using two different approaches such as cross flow filtration (CFF) system and chromatography methods. In the first approach, the protein was concentrated and partially purified through CFF to achieve maximum recovery yield and purity of 87 and 94 %, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329918/-endoscopic-ultrasound-guided-biliary-drainage
#4
REVIEW
Hyeong Seok Nam, Dae Hwan Kang
The therapeutic role of endoscopic ultrasound (EUS) has continued to evolve in recent years. EUS-guided biliary drainage (EUS-BD) can be performed as an effective alternative to percutaneous drainage or surgical options when conventional Endoscopic retrograde cholangiopancreatography fails or is not possible. Depending on the access and exit routes of the stent, multiple approaches to EUS-BD have been proposed. Each patient should receive an individualized approach based on the patient's condition, anatomy, and endoscopist's experience, with an appropriate backup prepared...
March 25, 2017: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
https://www.readbyqxmd.com/read/28329750/optoelectronic-plethysmography-in-clinical-practice-and-research-a-review
#5
Carlo Massaroni, Elena Carraro, Andrea Vianello, Sandra Miccinilli, Michelangelo Morrone, Irisz K Levai, Emiliano Schena, Paola Saccomandi, Silvia Sterzi, John W Dickinson, Samantha Winter, Sergio Silvestri
BACKGROUND: Optoelectronic plethysmography (OEP) is a non-invasive motion capture method to measure chest wall movements and estimate lung volumes. OBJECTIVES: To provide an overview of the clinical findings and research applications of OEP in the assessment of breathing mechanics across populations of healthy and diseased individuals. METHODS: A bibliographic research was performed with the terms "opto-electronic plethysmography," "optoelectronic plethysmography," and "optoelectronic plethysmograph" in 50 digital library and bibliographic search databases resulting in the selection of 170 studies...
March 23, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/28329636/correlation-between-cancer-incidences-and-google-searches-in-the-united-states
#6
Mackenzie R Wehner, Kevin T Nead, Eleni Linos
IntroductionDespite being highly prevalent, keratinocyte carcinomas (basal cell and squamous cell carcinomas lack nationwide registries. Internet search data has emerged as a new method to evaluate previously difficult to quantify public health outcomes and may be useful in keratinocyte carcinoma research.ObjectiveWe aimed to evaluate whether Google search density correlated with known incidences of common cancers in the United States.MethodsWe used the Center for Disease Control's National Program of Cancer Registries ageTadjusted cancer incidences (2008T2012 ...
September 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329454/adult-congenital-cardiac-care
#7
Brian E Kogon, Kati Miller, Paula Miller, Bahaaldin Alsoufi, Joshua M Rosenblum
BACKGROUND: The Adult Congenital Heart Association (ACHA) is dedicated to supporting patients with congenital heart disease. To guide patients to qualified providers and programs, it maintains a publicly accessible directory of dedicated adult congenital cardiac programs. We analyzed the directory in 2006 and 2015, aiming to evaluate the growth of the directory as a whole and to evaluate the growth of individual programs within the directory. We also hope this raises awareness of the growing opportunities that exist in adult congenital cardiology and cardiac surgery...
March 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/28329421/naproxen-inhibits-uvb-induced-basal-cell-and-squamous-cell-carcinoma-development-in-ptch1-skh-1-hairless-mice
#8
Sandeep C Chaudhary, Mohammad Waseem, Mehtab Rana, Hui Xu, Levy Kopelovich, Craig A Elmets, Mohammad Athar
Naproxen possesses anti-proliferative and pro-apoptotic effects besides its known anti-inflammatory functions. Here, we demonstrate the anti-cancer effects of naproxen against UVB-induced BCCs and SCCs in a highly susceptible murine model of UVB carcinogenesis. Naproxen significantly inhibited UVB-induced BCCs and SCCs in this model. Tumor number and volume were significantly decreased (p<0.005 and p<0.05, respectively). Inhibition in UVB-induced SCCs and BCCs was 77% and 86%, respectively, which was associated with reduced PCNA and cyclin D1 and increased apoptosis...
March 22, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28329385/importance-of-combined-left-atrial-size-and-estimated-pulmonary-pressure-for-clinical-outcome-in-patients-presenting-with-heart-failure-with-preserved-ejection-fraction
#9
Erwan Donal, Lars H Lund, Emmanuel Oger, Christian Bosseau, Amélie Reynaud, Camilla Hage, Elodie Drouet, J-Claude Daubert, Cecila Linde
Aims: Heart failure with preserved ejection fraction (HFpEF) is a complex syndrome with various phenotypes and outcomes. The prognostic relevance of echocardiography and the E/e' ratio has previously been reported. We sought to study in addition, the value of estimated pulmonary pressure and left atrial size for diagnosing and determining a prognosis for HFpEF-patients in a prospective multi-centric cohort. Methods and results: Patients with an acute-HF event accompanied with NT-proBNP >300 pg/mL (BNP >100 pg/mL) and LVEF >45% were included (n = 237) and clinically reassessed using echo-Doppler after 4-8 weeks of HF treatment as part of the prospective KaRen HFpEF study...
March 4, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329359/larger-lateral-prefrontal-cortex-volume-predicts-better-exercise-adherence-among-older-women-evidence-from-two-exercise-training-studies
#10
John R Best, Bryan K Chiu, Peter A Hall, Teresa Liu-Ambrose
Background: Recent research has suggested an important role of lateral prefrontal cortex (lPFC) in consistent implementation of positive health behaviors and avoidance of negative health behaviors. Methods: We examined whether gray matter volume in the lPFC prospectively predicts exercise class attendance among older women (n = 122) who underwent either a 52-week or 26-week exercise training intervention. Structural magnetic resonance imaging determined gray matter volume at baseline...
March 17, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28329301/association-between-non-iron-deficient-anemia-and-insomnia-symptoms-in-community-dwelling-older-adults-the-baltimore-longitudinal-study-of-aging
#11
Lenis P Chen-Edinboro, Laura E Murray-Kolb, Eleanor M Simonsick, Luigi Ferrucci, Richard Allen, Martha E Payne, Adam P Spira
Background: Anemia is associated with poorer sleep in children, and clinically, anemia is linked to insomnia. However, the association between anemia and insomnia in older adults is understudied. Methods: We examined the cross-sectional association between anemia and insomnia in 1,053 adults (71.4 ± 10.6 years) in the Baltimore Longitudinal Study of Aging. Participants were classified as nonanemic, non-iron-deficient anemic, or iron-deficient anemic based on hemoglobin, ferritin, transferrin saturation, and mean cell volume...
January 20, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28329299/rational-and-design-of-eurocrt-an-international-observational-study-on-multi-modality-imaging-and-cardiac-resynchronization-therapy
#12
Erwan Donal, Victoria Delgado, Julien Magne, Chiara Bucciarelli-Ducci, Christophe Leclercq, Bernard Cosyns, Marta Sitges, Thor Edvardsen, Elif Sade, Ivan Stankovic, Eustachio Agricola, Maurizio Galderisi, Patrizio Lancellotti, Alfredo Hernandez, Sven Plein, Denisa Muraru, Ehud Schwammenthal, Gerhard Hindricks, Bogdan A Popescu, Gilbert Habib
Aims: Assessment of left ventricular (LV) volumes and ejection fraction (LVEF) with cardiac imaging is important in the selection of patients for cardiac resynchronization therapy (CRT). Several observational studies have explored the role of imaging-derived LV dyssynchrony parameters to predict the response to CRT, but have yielded inconsistent results, precluding the inclusion of imaging-derived LV dyssynchrony parameters in current guidelines for selection of patients for CRT. METHODS: The EuroCRT is a large European multicentre prospective observational study led by the European Association of Cardiovascular Imaging...
February 27, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329275/tissue-characterization-by-t1-and-t2-mapping-cardiovascular-magnetic-resonance-imaging-to-monitor-myocardial-inflammation-in-healing-myocarditis
#13
S Bohnen, U K Radunski, G K Lund, F Ojeda, Y Looft, M Senel, L Radziwolek, M Avanesov, E Tahir, C Stehning, B Schnackenburg, G Adam, S Blankenberg, K Muellerleile
Aims: Monitoring disease activity in myocarditis is important for tailored therapeutic strategies. This study evaluated the ability of T1 and T2 mapping cardiovascular magnetic resonance (CMR) to monitor the course of myocardial inflammation in healing myocarditis. Methods and results: Forty-eight patients with strictly defined acute myocarditis underwent CMR at 1.5 T in the acute stage, at 3-months (n = 39), and at 12-months follow-up (FU) (n = 21). Normal values were obtained in a control group of 27 healthy subjects...
March 8, 2017: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329267/right-ventricular-recovery-after-bilateral-lung-transplantation-for-pulmonary-arterial-hypertension%C3%A2
#14
Thomas M Gorter, Erik A M Verschuuren, Dirk J van Veldhuisen, Elke S Hoendermis, Michiel E Erasmus, Harm J Bogaard, Anton Vonk Noordegraaf, Rolf M F Berger, Joost P van Melle, Tineke P Willems
OBJECTIVES: Pulmonary arterial hypertension (PAH) is a progressive and often fatal disease characterized by increased pulmonary vascular resistance (PVR) and right ventricular (RV) failure. End-stage PAH is often an indication for a lung transplant (LTX). Our goal was to study ventricular recovery using cardiac magnetic resonance imaging late after LTX. METHODS: We studied 10 patients with PAH who underwent isolated bilateral LTX. RV and left ventricular (LV) volumes, function and mass were measured...
March 3, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28329254/periodic-limb-movements-and-white-matter-hyperintensities-in-first-ever-minor-stroke-or-high-risk-tia
#15
Mark I Boulos, Brian J Murray, Ryan T Muir, Fuqiang Gao, Gregory M Szilagyi, Menal Huroy, Alexander Kiss, Arthur S Walters, Sandra E Black, Andrew S Lim, Richard H Swartz
Study Objectives: Emerging evidence suggests that periodic limb movements (PLMs) may contribute to the development of cerebrovascular disease. White matter hyperintensities (WMHs), a widely accepted biomarker for cerebral small vessel disease, are associated with incident stroke and death. We evaluated the association between increased PLM indices and WMH burden in patients presenting with stroke or transient ischemic attack (TIA), while controlling for vascular risk factors and stroke severity...
December 29, 2016: Sleep
https://www.readbyqxmd.com/read/28329240/effect-of-breathing-oxygen-enriched-air-on-exercise-performance-in-patients-with-precapillary-pulmonary-hypertension-randomized-sham-controlled-cross-over-trial
#16
Silvia Ulrich, Elisabeth D Hasler, Stéphanie Saxer, Michael Furian, Séverine Müller-Mottet, Stephan Keusch, Konrad E Bloch
Aims: The purpose of the current trial was to test the hypothesis that breathing oxygen-enriched air increases exercise performance of patients with pulmonary arterial or chronic thrombo-embolic pulmonary hypertension (PAH/CTEPH) and to investigate involved mechanisms. Methods and results: Twenty-two patients with PAH/CTEPH, eight women, means ± SD 61 ± 14 years, resting mPAP 35 ± 9mmHg, PaO2 ambient air >7.3 kPa, underwent four bicycle ergospirometries to exhaustion on different days, while breathing oxygen-enriched (FiO2 0...
March 11, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28329230/3d-echocardiographic-reference-ranges-for-normal-left-ventricular-volumes-and-strain-results-from-the-eacvi-norre-study
#17
Anne Bernard, Karima Addetia, Raluca Dulgheru, Luis Caballero, Tadafumi Sugimoto, Natela Akhaladze, George D Athanassopoulos, Daniele Barone, Monica Baroni, Nuno Cardim, Andreas Hagendorff, Krasimira Hristova, Federica Ilardi, Teresa Lopez, Gonzalo de la Morena, Bogdan A Popescu, Martin Penicka, Tolga Ozyigit, Jose David Rodrigo Carbonero, Nico van de Veire, Ralph Stephan Von Bardeleben, Dragos Vinereanu, Jose Luis Zamorano, Christophe Martinez, Julien Magne, Bernard Cosyns, Erwan Donal, Gilbert Habib, Luigi P Badano, Roberto M Lang, Patrizio Lancellotti
Aim: To obtain the normal ranges for 3D echocardiography (3DE) measurement of left ventricular (LV) volumes, function, and strain from a large group of healthy volunteers. Methods and results: A total of 440 (mean age: 45 ± 13 years) out of the 734 healthy subjects enrolled at 22 collaborating institutions of the Normal Reference Ranges for Echocardiography (NORRE) study had good-quality 3DE data sets that have been analysed with a vendor-independent software package allowing homogeneous measurements regardless of the echocardiographic machine used to acquire the data sets...
December 22, 2016: European Heart Journal Cardiovascular Imaging
https://www.readbyqxmd.com/read/28329201/video-assisted-thoracic-surgery-lobectomy-does-not-offer-any-functional-recovery-advantage-in-comparison-to-the-open-approach-3-months-after-the-operation-a-case-matched-analysis%C3%A2
#18
Michele Salati, Alessandro Brunelli, Francesco Xiumè, Marco Monteverde, Armando Sabbatini, Michela Tiberi, Cecilia Pompili, Roberto Palloni, Majed Refai
OBJECTIVES: The objective of the present study was to compare functional loss [forced expiratory volume in one second to forced vital capacity ratio (FEV1), DLCO and VO2max reduction] after VATS versus open lobectomies. METHODS: We performed a prospective observational study on 195 patients who had a pulmonary lobectomy from June 2010 to November 2014 and who were able to complete a 3-months functional evaluation follow-up program. Since the VATS technique was our first choice for performing lobectomies from January 2012, we divided the patients into two groups: the OPEN group (112 patients) and the VATS group (83 patients)...
February 23, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28329200/clinical-trends-in-surgical-minimally-invasive-and-transcatheter-aortic-valve-replacement%C3%A2
#19
Tom C Nguyen, Matthew D Terwelp, Vinod H Thourani, Yelin Zhao, Nidal Ganim, Carson Hoffmann, Monica Justo, Anthony L Estrera, Richard W Smalling, Prakash Balan, Joseph Lamelas
OBJECTIVES: Transcatheter aortic valve replacement (TAVR) and minimally invasive aortic valve replacement (MIAVR) have emerged as alternatives to surgical aortic valve replacement (SAVR) via traditional sternotomy. However, their effect on clinical practice remains unclear. The study's objective is to describe clinical trends between TAVR, MIAVR and SAVR in patients with severe aortic stenosis (AS). METHODS: This retrospective observational study analyzed trends in isolated severe aortic valve replacement (AVR) among three high volume TAVR, MIAVR and SAVR centres in the United States...
February 20, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28329193/a-successful-transdiaphragmatic-shunting-for-a-late-and-recurrent-pericardial-effusion-following-coronary-bypass
#20
Nicolas Themelin, Serge Cappeliez, Jean-Jacques Houben, Badih El Nakadi
Although pericardial effusion (PE) is common after cardiac surgery, late and recurrent cardiac tamponade that occurs more than 5-7 days after coronary artery bypass grafting is an infrequent complication. Moreover, the treatment of PE resistant to medical therapy, percutaneous drainage and pleuro-pericardial window remains a therapeutic challenge. We report the case of a recalcitrant PE with high-daily volume outflow drainage, finally treated with success, with a transdiaphragmatic pleuro-peritoneal shunting by laparoscopic approach...
February 16, 2017: Interactive Cardiovascular and Thoracic Surgery
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