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https://www.readbyqxmd.com/read/28212929/structural-analysis-of-a-homogeneous-polysaccharide-from-achatina-fulica
#1
Jie Liu, Feigneng Shang, Zengming Yang, Mingyi Wu, Jinhua Zhao
Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structural of the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy ((1)H, (13)C, COSY, TOCSY, NOESY, HSQC and HMBC)...
February 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28211963/a-parametric-model-of-the-brain-vascular-system-for-estimation-of-the-arterial-input-function-aif-at-the-tissue-level
#2
Siamak P Nejad-Davarani, Hassan Bagher-Ebadian, James R Ewing, Douglas C Noll, Tom Mikkelsen, Michael Chopp, Quan Jiang
In this paper, we introduce a novel model of the brain vascular system, which is developed based on laws of fluid dynamics and vascular morphology. This model is used to address dispersion and delay of the arterial input function (AIF) at different levels of the vascular structure and to estimate the local AIF in DCE images. We developed a method based on the simplex algorithm and Akaike information criterion to estimate the likelihood of the contrast agent concentration signal sampled in DCE images belonging to different layers of the vascular tree or being a combination of different signal levels from different nodes of this structure...
February 17, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28211961/an-extended-vascular-model-for-less-biased-estimation-of-permeability-parameters-in-dce-t1-images
#3
Siamak P Nejad-Davarani, Hassan Bagher-Ebadian, James R Ewing, Douglas C Noll, Tom Mikkelsen, Michael Chopp, Quan Jiang
One of the key elements in dynamic contrast enhanced (DCE) image analysis is the arterial input function (AIF). Traditionally, in DCE studies a global AIF sampled from a major artery or vein is used to estimate the vascular permeability parameters; however, not addressing dispersion and delay of the AIF at the tissue level can lead to biased estimates of these parameters. To find less biased estimates of vascular permeability parameters, a vascular model of the cerebral vascular system is proposed that considers effects of dispersion of the AIF in the vessel branches, as well as extravasation of the contrast agent (CA) to the extravascular-extracellular space...
February 17, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28211838/work-satisfaction-burnout-and-gender-based-inequalities-among-ophthalmologists-in-india-a-survey
#4
Akshay Gopinathan Nair, Pooja Jain, Aniruddha Agarwal, Vandana Jain
BACKGROUND: Ophthalmology is a rapidly evolving branch of medicine and advancing technology has raised the bar of patient expectations and outcomes. However, studies that assess physician stress and satisfaction especially in developing countries are limited in literature. OBJECTIVE: This index study aims at looking at the levels of job satisfaction, burnout and perception of gender disparity among ophthalmologists in India. METHODS: An Internet-based survey was sent out to ophthalmologists...
February 17, 2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/28211706/observation-of-d-0-%C3%A2-%C3%AF-0-%C3%AE-and-search-for-cp-violation-in-radiative-charm-decays
#5
T Nanut, A Zupanc, I Adachi, H Aihara, S Al Said, D M Asner, V Aulchenko, T Aushev, R Ayad, V Babu, I Badhrees, A M Bakich, V Bansal, P Behera, V Bhardwaj, J Biswal, A Bondar, A Bozek, M Bračko, T E Browder, D Červenkov, V Chekelian, A Chen, B G Cheon, R Chistov, K Cho, S-K Choi, Y Choi, D Cinabro, N Dash, S Di Carlo, Z Doležal, D Dutta, S Eidelman, H Farhat, J E Fast, T Ferber, B G Fulsom, V Gaur, N Gabyshev, A Garmash, R Gillard, P Goldenzweig, B Golob, K Hayasaka, H Hayashii, W-S Hou, T Iijima, K Inami, G Inguglia, A Ishikawa, Y Iwasaki, W W Jacobs, I Jaegle, D Joffe, K K Joo, T Julius, A B Kaliyar, K H Kang, T Kawasaki, D Y Kim, J B Kim, K T Kim, M J Kim, S H Kim, K Kinoshita, P Kodyš, S Korpar, P Krokovny, T Kuhr, R Kulasiri, A Kuzmin, Y-J Kwon, J S Lange, I S Lee, C H Li, L Li, Y Li, L Li Gioi, J Libby, D Liventsev, M Lubej, M Masuda, T Matsuda, D Matvienko, K Miyabayashi, H Miyata, R Mizuk, G B Mohanty, H K Moon, M Nakao, K J Nath, M Nayak, N K Nisar, S Nishida, S Ogawa, S Okuno, P Pakhlov, G Pakhlova, B Pal, C-S Park, C W Park, H Park, S Paul, T K Pedlar, L Pesántez, R Pestotnik, M Petrič, L E Piilonen, K Prasanth, C Pulvermacher, J Rauch, M Ritter, A Rostomyan, Y Sakai, S Sandilya, L Santelj, T Sanuki, Y Sato, V Savinov, T Schlüter, O Schneider, G Schnell, C Schwanda, A J Schwartz, Y Seino, K Senyo, O Seon, M E Sevior, V Shebalin, C P Shen, T-A Shibata, J-G Shiu, B Shwartz, E Solovieva, S Stanič, M Starič, J F Strube, J Stypula, T Sumiyoshi, M Takizawa, U Tamponi, F Tenchini, K Trabelsi, M Uchida, S Uno, Y Ushiroda, G Varner, A Vinokurova, V Vorobyev, A Vossen, C H Wang, M-Z Wang, P Wang, Y Watanabe, E Widmann, E Won, J Yamaoka, Y Yamashita, J Yelton, Z P Zhang, V Zhilich, V Zhukova, V Zhulanov
We report the first observation of the radiative charm decay D^{0}→ρ^{0}γ and the first search for CP violation in decays D^{0}→ρ^{0}γ, ϕγ, and K[over ¯]^{*0}(892)γ, using a data sample of 943  fb^{-1} collected with the Belle detector at the KEKB asymmetric-energy e^{+}e^{-} collider. The branching fraction is measured to be B(D^{0}→ρ^{0}γ)=(1.77±0.30±0.07)×10^{-5}, where the first uncertainty is statistical and the second is systematic. The obtained CP asymmetries A_{CP}(D^{0}→ρ^{0}γ)=+0...
February 3, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28210761/the-evolutionary-loss-of-rnai-key-determinants-in-kinetoplastids-as-a-multiple-sporadic-phenomenon
#6
Andrey V Matveyev, João M P Alves, Myrna G Serrano, Vladimir Lee, Ana M Lara, William A Barton, André G Costa-Martins, Stephen M Beverley, Erney P Camargo, Marta M G Teixeira, Gregory A Buck
We screened the genomes of a broad panel of kinetoplastid protists for genes encoding proteins associated with the RNA interference (RNAi) system using probes from the Argonaute (AGO1), Dicer1 (DCL1), and Dicer2 (DCL2) genes of Leishmania brasiliensis and Crithidia fasciculata. We identified homologs for all the three of these genes in the genomes of a subset of these organisms. However, several of these organisms lacked evidence for any of these genes, while others lacked only DCL2. The open reading frames encoding these putative proteins were structurally analyzed in silico...
February 16, 2017: Journal of Molecular Evolution
https://www.readbyqxmd.com/read/28210202/mechanisms-and-effects-of-transcranial-direct-current-stimulation
#7
James Giordano, Marom Bikson, Emily S Kappenman, Vincent P Clark, H Branch Coslett, Michael R Hamblin, Roy Hamilton, Ryan Jankord, Walter J Kozumbo, R Andrew McKinley, Michael A Nitsche, J Patrick Reilly, Jessica Richardson, Rachel Wurzman, Edward Calabrese
The US Air Force Office of Scientific Research convened a meeting of researchers in the fields of neuroscience, psychology, engineering, and medicine to discuss most pressing issues facing ongoing research in the field of transcranial direct current stimulation (tDCS) and related techniques. In this study, we present opinions prepared by participants of the meeting, focusing on the most promising areas of research, immediate and future goals for the field, and the potential for hormesis theory to inform tDCS research...
January 2017: Dose-response: a Publication of International Hormesis Society
https://www.readbyqxmd.com/read/28209099/does-calcium-score-in-great-pelvic-vessels-predict-colorectal-anastomotic-leakage-a-prospective-study-of-one-hundred-anastomoses
#8
M T Norooz, H Moradi, M Safdarian, F Jahangiri, H A Amoli
BACKGROUND: Anastomotic leakage is one of the most severe surgical complications following surgery. This prospective study was designed to investigate an association between the calcification in the descending aorta and its major branches using a calcium-scoring software and colorectal anastomotic leakage. METHODS: From January 2012 to March 2013, one hundred patients underwent surgeries involving colorectal anastomosis procedures. Calcium score in descending aorta and great pelvic vessels was measured using the Syngo-CT 2006G-W software...
September 2016: Acta Gastro-enterologica Belgica
https://www.readbyqxmd.com/read/28208462/beyond-flory-theory-distribution-functions-for-interacting-lattice-trees
#9
Angelo Rosa, Ralf Everaers
While Flory theories [J. Isaacson and T. C. Lubensky, J. Physique Lett. 41, 469 (1980)JPSLBO0302-072X10.1051/jphyslet:019800041019046900; M. Daoud and J. F. Joanny, J. Physique 42, 1359 (1981)JOPQAG0302-073810.1051/jphys:0198100420100135900; A. M. Gutin et al., Macromolecules 26, 1293 (1993)MAMOBX0024-929710.1021/ma00058a016] provide an extremely useful framework for understanding the behavior of interacting, randomly branching polymers, the approach is inherently limited. Here we use a combination of scaling arguments and computer simulations to go beyond a Gaussian description...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28208149/distal-middle-cerebral-artery-branch-recanalization-does-not-affect-final-lesion-volume-in-ischemic-stroke
#10
Verena van Ginneken, Daniel Gierhake, Heinrich J Audebert, Jochen B Fiebach
BACKGROUND: Early recanalization in ischemic stroke is associated with favorable outcome. However, limited data are available on the effect of recanalization on infarct growth and functional outcome in stroke with distal middle cerebral artery (MCA) pathology. This study was aimed at determining the effect of recanalization in acute stroke patients with perfusion-diffusion mismatch and occlusion or high-grade stenosis of a distal MCA branch. METHODS: We prospectively examined 34 consecutive stroke patients with perfusion-diffusion mismatch and M3 or M4 pathology within 24 h of symptom onset...
February 17, 2017: Cerebrovascular Diseases
https://www.readbyqxmd.com/read/28207967/the-synonymy-of-the-toxic-dinoflagellates-prorocentrum-mexicanum-and-p-rhathymum-and-the-description-of-p-steidingerae-sp-nov-prorocentrales-dinophyceae
#11
Fernando Gómez, Dajun Qiu, Senjie Lin
Prorocentrum mexicanum and P. rhathymum are toxicologically important dinoflagellates, but their relationship is not well defined. We investigated strains from Puerto Rico and Brazil by light and scanning electron microscopies. We provide molecular data from a strain isolated near the type locality of P. rhathymum, and the first morphological and molecular data from the South Atlantic Ocean. The rRNA gene (rDNA) sequences of the Puerto Rican and Brazilian strains were identical, and their morphologies fit the description of P...
February 16, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28207539/role-of-coronary-angiography-for-out-of-hospital-cardiac-arrest-survivors-according-to-postreturn-of-spontaneous-circulation-on-an-electrocardiogram
#12
Tae Rim Lee, Sung Yeon Hwang, Won Chul Cha, Tae Gun Shin, Min Seob Sim, Ik Joon Jo, Keun Jeong Song, Joong Eui Rhee, Yeon Kwon Jeong
Survivors of out-of-hospital cardiac arrest (OHCA) have high mortality and morbidity. An acute coronary event is the most common cause of sudden cardiac death. For this reason, coronary angiography is an important diagnostic and treatment strategy for patients with postcardiac arrest. This study aimed to identify the correlation between postreturn of spontaneous circulation (ROSC) on an electrocardiogram (ECG) and results of coronary angiography of OHCA survivors.We collected data from our OHCA registry from January 2010 to November 2014...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28205344/utility-of-a-standardized-postcardiopulmonary-bypass-epicardial-echocardiography-protocol-for-stage-i-norwood-palliation
#13
Kenan W D Stern, Kimberlee Gauvreau, Sitaram Emani, Tal Geva
OBJECTIVE: Stage 1 Norwood palliation is one of the highest risk procedures in congenital cardiac surgery. Patients with superior technical performance scores have more favorable outcomes. Intraoperative epicardial echocardiography may allow the surgeon to address residual lesions prior to leaving the operating room, resulting in improved technical performance. The ability of intraoperative epicardial echocardiography to visualize the relevant anatomy and its association with outcomes is not known...
February 16, 2017: Congenital Heart Disease
https://www.readbyqxmd.com/read/28203619/variation-in-the-response-of-eastern-and-southern-africa-provenances-of-faidherbia-albida-delile-a-chev-seedlings-to-water-supply-a-greenhouse-experiment
#14
Grace Koech, Daniel Ofori, Anne W T Muigai, Jonathan Muriuki, Parveen Anjarwalla, Jan De Leeuw, Jeremias G Mowo
Rural communities value Faidherbia albida in farming systems and pastoralism. Faidherbia albida provides products such as medicine, fodder, fuel, wood, food and services such as shade, soil fertility and nutrient cycling. Excessive browsing by animals, branch lopping and pod harvesting, have critically reduced the natural regeneration in some areas which exposes it to challenges due to dependence upon natural regeneration. The objective of this research was to evaluate response of Faidherbia albida provenances from eastern (Taveta Wangingombe) and southern Africa (Lupaso, Kuiseb Manapools) to different watering regimes to aid in selection of provenances for domestication...
October 2016: Global Ecology and Conservation
https://www.readbyqxmd.com/read/28202628/effects-of-upgrade-versus-de-novo-cardiac-resynchronization-therapy-on-clinical-response-and-long-term-survival-results-from-a-multicenter-study
#15
Mate Vamos, Julia W Erath, Zsolt Bari, Denes Vagany, Sven P Linzbach, Tatsiana Burmistrava, Carsten W Israel, Gabor Z Duray, Stefan H Hohnloser
BACKGROUND: Benefits of cardiac resynchronization therapy (CRT) on morbidity and mortality in selected patients are well known. Although the number of upgrade procedures from single- or dual-chamber devices to CRT is increasing, there are only sparse data on the outcomes of upgrade procedures compared with de novo CRT. This study aimed to evaluate clinical response and survival in patients receiving de novo versus upgrade CRT defibrillator therapy. METHODS AND RESULTS: Prospectively collected outcome data were compared in patients undergoing de novo or upgrade CRT defibrillator implantation at 3 implant centers in Germany and Hungary...
February 2017: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/28202214/lethal-neonatal-case-and-review-of-primary-short-chain-enoyl-coa-hydratase-sceh-deficiency-associated-with-secondary-lymphocyte-pyruvate-dehydrogenase-complex-pdc-deficiency
#16
Jirair K Bedoyan, Samuel P Yang, Sacha Ferdinandusse, Rhona M Jack, Alexander Miron, George Grahame, Suzanne D DeBrosse, Charles L Hoppel, Douglas S Kerr, Ronald J A Wanders
Mutations in ECHS1 result in short-chain enoyl-CoA hydratase (SCEH) deficiency which mainly affects the catabolism of various amino acids, particularly valine. We describe a case compound heterozygous for ECHS1 mutations c.836T>C (novel) and c.8C>A identified by whole exome sequencing of proband and parents. SCEH deficiency was confirmed with very low SCEH activity in fibroblasts and nearly absent immunoreactivity of SCEH. The patient had a severe neonatal course with elevated blood and cerebrospinal fluid lactate and pyruvate concentrations, high plasma alanine and slightly low plasma cystine...
February 2, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28201989/revisiting-phylogenetic-signal-strong-or-negligible-impacts-of-polytomies-and-branch-length-information
#17
Rafael Molina-Venegas, Miguel Á Rodríguez
BACKGROUND: Inaccurate estimates of phylogenetic signal may mislead interpretations of many ecological and evolutionary processes, and hence understanding where potential sources of uncertainty may lay has become a priority for comparative studies. Importantly, the sensitivity of phylogenetic signal indices and their associated statistical tests to incompletely resolved phylogenies and suboptimal branch-length information has been only partially investigated. METHODS: Here, we use simulations of trait evolution along phylogenetic trees to assess whether incompletely resolved phylogenies (polytomic chronograms) and phylogenies with suboptimal branch-length information (pseudo-chronograms) could produce directional biases in significance tests (p-values) associated with Blomberg et al...
February 15, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28199850/flow-dynamics-and-hspc-homing-in-bone-marrow-microvessels
#18
M Gabriele Bixel, Anjali P Kusumbe, Saravana K Ramasamy, Kishor K Sivaraj, Stefan Butz, Dietmar Vestweber, Ralf H Adams
Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28199774/sarcopenia-predicts-minimal-hepatic-encephalopathy-in-patients-with-liver-cirrhosis
#19
Tatsunori Hanai, Makoto Shiraki, Satoshi Watanabe, Takahiro Kochi, Kenji Imai, Atsushi Suetsugu, Koji Takai, Hisataka Moriwaki, Masahito Shimizu
AIM: Minimal hepatic encephalopathy (MHE) and sarcopenia impair the health-related quality of life and prognosis in patients with liver cirrhosis; however, the relationship between MHE and sarcopenia remains unclear. The aim of this study was to investigate their relationship and to identify the predictors of MHE in cirrhotic patients. METHODS: This retrospective study evaluated 120 cirrhotic patients who were tested for MHE and sarcopenia. MHE was diagnosed by using the computer-aided neuropsychiatric test...
February 15, 2017: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/28198771/correlation-between-facial-nerve-axonal-load-and-age-and-its-relevance-to-facial-reanimation
#20
Austin Hembd, Purushottam Nagarkar, Justin Perez, Andrew Gassman, Philip Tolley, Joan Reisch, Charles L White, Shai Rozen
PURPOSE: Two-stage facial reanimation procedures with a cross-facial nerve graft often have unsatisfactory results in the older patient. Although the cause of result variability is likely multifactorial, some studies suggest that increased donor nerve axonal load improves function of a free muscle transfer after a cross-facial nerve graft. This study attempts to characterize the relationship between age and facial nerve axonal load. METHODS: 63 fresh, cadaveric heads were dissected to expose the facial nerve...
February 13, 2017: Plastic and Reconstructive Surgery
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