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Halima Meriem Issaadi, Yu-Chi Tsai, Fang-Rong Chang, Attila Hunyadi
Phytoecdysteroids are known for their various beneficial bioactivities in mammals including a non-hormonal anabolic and adaptogenic effect. Cyanotis arachnoidea extracts are extensively utilized worldwide as ecdysteroid-rich materials for various purposes, e.g. food supplementation, use in agriculture and aquaculture, etc. Preparative chromatography of ecdysteroids requires extensive use of methods of different selectivity, and only a very limited number of papers are available on related application of modern liquid-liquid chromatographic techniques...
June 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Attila Hunyadi, Ibolya Herke, Katalin Lengyel, Mária Báthori, Zoltán Kele, András Simon, Gábor Tóth, Kálmán Szendrei
Phytoecdysteroids like 20-hydroxyecdysone ("ecdysterone") can exert a mild, non-hormonal anabolic/adaptogenic activity in mammals, and as such, are frequently used in food supplements. Spinach is well-known for its relatively low ecdysteroid content. Cyanotis arachnoidea, a plant native in China, is among the richest sources of phytoecdysteroids, and extracts of this plant are marketed in tons per year amounts via the internet at highly competitive prices. Here we report the investigation of a series of food supplements produced in Germany and claimed to contain spinach extracts...
December 8, 2016: Scientific Reports
Deyong Li, Yawei Zhang, Zhenliang Cui, Liwen He, Wanbao Chen, Qingxiang Meng, Liping Ren
The objective of this study was to evaluate the effects of supplementation of phytoecdysteroids (PEDS) extracted from Cyanotis arachnoidea on rumen fermentation, enzymes activity and microbial efficiency in a dual flow continuous-culture system. A single-factor experimental design was used with twelve fermenters in 4 groups with 3 replicates each. Fermenters were incubated for a total of 7 days that included first 4 days for adaptation and last 3 days for sampling. PEDS was added at levels of zero (as control), 5, 10, and 15 mg/g of the substrate (DM)...
2016: PloS One
Qiu Jun Wang, Li Ping Zheng, Pei Fei Zhao, Yi Lu Zhao, Jian Wen Wang
Cyanotis arachnoidea contains a rich source of bioactive phytoecdysteroids (i.e. analogues of insect steroid hormones). 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) supplies mevalonate for the synthesis of many secondary metabolites including 20-hydroxyecdysone (20E), one of metabolism-enhancing phytoecdysteroids. In this study, in order to develop a sustainable source of 20E, cell suspension cultures were established from shoot cultures of C. arachnoidea, and a full length cDNA encoding HMGR (designated as CaHMGR) was cloned and characterized...
November 2014: Plant Physiology and Biochemistry: PPB
Chengyu Tan, Liang Kong, Xi Li, Wei Li, Ning Li
Cyanotis arachnoidea is a plant with plenty of phytoecdysteroid. To study the active compound in it, a new phytoecdysteroid with 5alpha-cholesta skeleton, was isolated from the whole plant of Cyanotis arachnoidea C. B. Clarke by using various chromatographic methods (alumina column chromatography, silica gel column chromatography, octadecyl silane (ODS) column chromatography, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)). Its structure was analyzed on the basis of 1D and 2D nuclear magnetic resonances (NMR), electrospray ionization mass spectrometry (ESI-MS) methods...
September 2011: Se Pu, Chinese Journal of Chromatography
A Toon, J M Hughes, L Joseph
Multilocus studies in phylogenetics and comparative phylogeography have the power to explore a broader spectrum of evolutionary questions than either discipline has alone. To examine the origins of sympatry in a group of closely related birds of mostly mesic eucalypt woodlands in Australia, we reconstructed the relationships among species of Entomyzon and Melithreptus honeyeaters (Aves: Passeriformes: Meliphagidae) using a mitochondrial marker, ND2, and six non-coding nuclear loci (total 4719 base pairs). By sampling across the geographical range of each species, we studied not only their phylogenetic relationships to each other but also the spatial distribution of their genetic diversity...
July 2010: Molecular Ecology
Sophie Crouzet, Annick Maria, Laurence Dinan, René Lafont, Jean-Pierre Girault
Cyanotis longifolia Benth. (Commelinaceae) contains ecdysteroids, which are highly concentrated in the roots and flowers, whereas leaves contain only very low amounts and stems intermediate amounts. 20-Hydroxyecdysone is the major component found in all tissues, but roots also contain large amounts of 20-hydroxyecdysone 3-acetate and ajugasterone C. A preparative experiment has shown that roots contain a complex ecdysteroid mixture, and the analysis of minor components has allowed the isolation of several already known ecdysteroids (polypodine B, 2-deoxy-20,26-dihydroxyecdysone, isovitexirone, poststerone) together with five new (ajugasterone C 3-acetate, 5beta-hydroxy-poststerone, poststerone 2-acetate, 14(15)-dehydro-poststerone 2-acetate, 24-epi-atrotosterone A [=24-methyl-ajugasterone C]) ecdysteroids that have been fully characterized...
December 2009: Archives of Insect Biochemistry and Physiology
Rudayna Ghubash
Parasites are a common cause of dermatological disease in the dog and cat. Knowledge of different miticidal options for the common parasitic diseases is imperative when choosing the appropriate treatment for a patient. This is especially important with the recent advent of safer and more effective antiparasitic medications. Diagnostic and therapeutic methods for Cheyletiella spp., Demodex spp., Notoedres cati, Sarcoptes scabei, and Otodectes cyanotis are discussed, with emphasis on protocols for miticidal therapies, as well as safety concerns and side effects...
August 2006: Clinical Techniques in Small Animal Practice
No abstract text is available yet for this article.
March 15, 1952: Nature
Cheng-yu Tan, Jin-hui Wang, Xian Li, Yu-guang Du, Xue-fang Bai
AIM: To investigate the chemical constituents of Cyanotis arachnoidea. METHODS: By using chromatographic methods for separation and combination with spectral analysis, their chemical structures were determined. RESULTS: Six compounds were identified as ajugasterone C-20, 22-acetonide (1), 20-hydroxyecdysone-20, 22-acetonide (2), 22-oxo-ajugasterone C (3), 22-oxo-20-hydroxyecdysone (4), beta-sitosterol (5), daucosterol (6). CONCLUSION: Compound 3 is a new compound, 4 was a new natural compound...
October 2003: Yao Xue Xue Bao, Acta Pharmaceutica Sinica
Cheng-Yu Tan, Jin-Hui Wang, Xian Li
Four phytoecdysteroids that have only 19 or 21 carbons, named 11alpha-hydroxyrubrosterone (1), dihydroxyrubrosterone (2), rubrosterone (3) and poststerone (4), were isolated from the whole plant of Cyanotis arachnoidea C.B. Clarke. Among them, 1 was a new compound. Their structures were elucidated by spectroscopic methods.
December 2003: Journal of Asian Natural Products Research
Cheng-Yu Tan, Jin-Hui Wang, Hui Zhang, Xian Li
From Cyanotis arachnoidea C.B. Clarke, a new phytosterone named cyanosterone A (1), along with three known compounds--beta-ecdysone (2), ajugasterone C (3) and beta-sitosterol (4) were isolated. The structure of the new compound was determined as 3beta,14alpha,20R,22R-tetrahydroxy-5alpha-cholest-7-en-6-one on the basis of physicochemical properties and spectral analysis.
March 2002: Journal of Asian Natural Products Research
A D Larkin, G E Gaillard
Dermatophagoides had been established as an antigen in house dust by many investigators. An untapped source of cross antigenicity is presented in this paper, namely, Otodectes cyanotis, the mite found in the ears of millions of cats. House dust samples for mite species in other parts of the world may not be similar to samples in the United States since in most other countries the cat exists outdoors, whereas in this country it is a household pet.
June 1981: Annals of Allergy
A C Santos, M T Chua, N Eufemio, C Abela
No abstract text is available yet for this article.
October 15, 1970: Experientia
B Goldschmidt, J Siegler, M Schmidt
The metabolism and in vivo kinetics of fibrinogen labelled with radioactive iodine was studied in children with cyanotic congenital heart disease. The patients had a significantly lowered plasma fibrinogen pool, shortened fibrinogen half-life and increased fractional catabolic rate of fibrinogen compared with healthy children. The average plasma fibrinogen and absolute catabolic rate of fibrinogen did not differ from control values. The shortened fibrinogen half-life together with the correcting effect of anticoagulation with heparin indicated that fibrinogen was consumed by chronic disseminated intravascular coagulation...
1977: Haematologia
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