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https://www.readbyqxmd.com/read/28331099/interferon-inducible-oligoadenylate-synthetase-like-protein-acts-as-an-antiviral-effector-against-classical-swine-fever-virus-via-the-mda5-mediated-type-i-interferon-signaling-pathway
#1
Lian-Feng Li, Jiahui Yu, Yuexiu Zhang, Qian Yang, Yongfeng Li, Lingkai Zhang, Jinghan Wang, Su Li, Yuzi Luo, Yuan Sun, Hua-Ji Qiu
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which poses a serious threat to the global pig industry. Interferons (IFNs) and IFN-stimulated genes (ISGs) play a key role in host antiviral defense. We have previously screened the porcine 2' -5' -oligoadenylate synthase-like protein (pOASL) as a potential anti-CSFV ISG using a reporter CSFV. This study aimed to clarify the underlying antiviral mechanism of pOASL against CSFV. We confirmed that CSFV replication was significantly suppressed in lentivirus-delivered, pOASL-overexpressing PK-15 cells, whereas silencing the expression of endogenous pOASL by small interfering RNAs markedly enhanced CSFV growth...
March 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28291628/invitro-activity-of-imipenem-relebactam-against-gram-negative-bacilli-isolated-from-patients-with-lower-respiratory-tract-infections-in-the-united-states-in-2015-results-from-the-smart-global-surveillance-program
#2
Sibylle H Lob, Meredith A Hackel, Krystyna M Kazmierczak, Daryl J Hoban, Katherine Young, Mary R Motyl, James A Karlowsky, Daniel F Sahm
The β-lactamase inhibitor relebactam inactivates class A β-lactamases, including KPC-type carbapenemases, and class C β-lactamases. Relebactam combined with imipenem is in clinical development for several indications, including hospital-acquired and ventilator-associated pneumonia. Employing CLSI-defined broth microdilution methodology, we evaluated the activities of imipenem-relebactam (using imipenem MIC breakpoints) and comparators against non-Proteeae Enterobacteriaceae (n=853) and Pseudomonas aeruginosa (n=598) isolated from lower respiratory tract infection samples in 20 hospital laboratories in the United States participating in the 2015 SMART (Study for Monitoring Antimicrobial Resistance Trends) global surveillance program...
March 2, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28271723/interaction-of-iron-nanoparticles-with-nervous-system-an-invitro-study
#3
Gelare Hajsalimi, Saba Taheri, Farshad Shahi, Leila Pishkar, Farnoosh Attar, Hosein Ahmadi, Mojtaba Falahati
Nanoparticles (NPs) are one of interesting and widely studying issues mainly because of their particular physico-chemical features and broad applications in the field of biomedical sciences, such as diagnosis and drug delivery. In this study, the interaction of iron nanoparticles (Fe-NPs) with Tau protein and PC12 cell, as potential nervous system models, was investigated with a range of techniques including dynamic light scattering (DLS), intrinsic fluorescence spectroscopy, circular dichroism (CD), [(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium-bromid] (MTT) assay, and acridine orange/ethidium bromide (AO/EB) dual staining method...
March 8, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28259758/huntingtin-associated-protein-1-hap1-regulates-endocytosis-and-interacts-with-multiple-trafficking-related-proteins
#4
Kimberly D Mackenzie, Yoon Lim, Michael D Duffield, Timothy Chataway, Xin-Fu Zhou, Damien J Keating
Huntingtin-associated protein 1 (HAP1) was initially identified as a binding partner of huntingtin, mutations in which underlie Huntington's disease. Subcellular localization and protein interaction data indicate that HAP1 may be important in vesicle trafficking, cell signalling and receptor internalization. In this study, a proteomics approach was used for the identification of novel HAP1-interacting partners to attempt to shed light on the physiological function of HAP1. Using affinity chromatography with HAP1-GST protein fragments bound to Sepharose columns, this study identified a number of trafficking-related proteins that bind to HAP1...
March 1, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28250133/mosgctl-7-a-c-type-lectin-protein-mediates-japanese-encephalitis-virus-infection-in-mosquitoes
#5
Ke Liu, Yingjuan Qian, Yong-Sam Jung, Bin Zhou, Ruibing Cao, Ting Shen, Donghua Shao, Jianchao Wei, Zhiyong Ma, Puyan Chen, Huaimin Zhu, Yafeng Qiu
Japanese encephalitis virus (JEV) is an arthropod-borne flavivirus prevalent in Asia and the Western Pacific, and is the leading cause of viral encephalitis. JEV is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. We investigated the expression of all C-type lectins during JEV infection in Aedes aegypti. C-type lectin mosGCTL-7 (AAEL002524) was significantly up-regulated by JEV infection and facilitated infection in vivo and in vitro...
March 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28221765/design-of-macroscopically-ordered-liquid-crystalline-hydrogel-columns-knitted-with-nanosilver-for-topical-applications
#6
Reshma Lali Raveendran, Nishanth Kumar Sasidharan, Sudha J Devaki
The design of liquid crystalline hydrogels knitted with silver nanoparticles in macroscopic ordering is becoming a subject of research interest due to their promising multifunctional applications in biomedical and optoelectronic applications. The present work describes the development of liquid crystalline Schiff-based hydrogel decorated with silver nanoparticles and the demonstration of its antifungal applications. Schiff base was prepared from polyglucanaldehyde and chitosan, and the former was prepared by the oxidation of amylose (polyglucopyranose) isolated from abundantly available unutilized jackfruit seed starch...
March 1, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28194552/a-novel-synthetic-small-molecule-yf-452-inhibits-tumor-growth-through-antiangiogenesis-by-suppressing-vegf-receptor-2-signaling
#7
Yongrui Liu, Yuan He, Feifei Yang, Xiaonan Cong, Jinhua Wang, Shihong Peng, Dan Gao, Weifang Wang, Liping Lan, Xuexiang Ying, Mingyao Liu, Yihua Chen, Zhengfang Yi
Tumor angiogenesis is characterized by abnormal vessel morphology, endowing tumor with highly hypoxia and unresponsive toward treatment. To date, mounting angiogenic factors have been discovered as therapeutic targets in antiangiogenic drug development. Among them, vascular endothelial growth factor receptor 2 (VEGFR2) inhibitors exerts potent antiangiogenic activity in tumor therapy. Therefore, it may provide a valid strategy for cancer treatment through targeting the tumor angiogenesis via VEGFR2 pathway...
February 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28137364/nucleoplasmic-bridges-as-a-biomarker-of-dna-damage-exposed-to-radon
#8
C Meenakshi, K Sivasubramanian, B Venkatraman
Radon is a naturally occurring radionuclide in the environment, during decay it emits high linear energy transfer (LET) alpha particles. When radon exposure is accompanied by smoking it has been reported that lung cancer risk is higher. Blood samples were collected after prior consent, 25 smokers and 25 non smokers (only males) exposed in vitro to radon gas with doses ranging between 0.3-12.6mGy Ionizing radiation is a strong clastogenic agent and a potent inducer of MN. Cytokinesis-Blocked Micro Nucleus (CBMN) assay has proven to be a reliable, thoroughly validated and standardised technique in the field of radiation biology...
February 2017: Mutation Research
https://www.readbyqxmd.com/read/28089609/de-immunized-and-functional-therapeutic-deft-versions-of-a-long-lasting-recombinant-alpha-interferon-for-antiviral-therapy
#9
Eduardo F Mufarrege, Sofía Giorgetti, Marina Etcheverrigaray, Frances Terry, William Martin, Anne S De Groot
Interferon α (IFN-α) exerts potent antiviral, immunomodulatory, and antiproliferative activity and have proven clinical utility in chronic hepatitis B and C virus infections. However, repeated IFN-α administration induces neutralizing antibodies (NAb) against the therapeutic in a significant number of patients. Associations between IFN-α immunogenicity and loss of efficacy have been described. So as to improve the in vivo biological efficacy of IFN-α, a long lasting hyperglycosylated protein (4N-IFN) derived from IFN-α2b wild type (WT-IFN) was developed...
January 10, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28048947/effect-of-implant-position-and-span-length-on-stresses-around-implants-assisting-claspless-distal-extension-partial-overdentures-an-invitro-study
#10
Moustafa Abdou Elsyad, Ahmed Kabeel, Nesreen Elmekawy
The aim of this invitro study was to evaluate the effect of implant position and edentulous span length on stresses around implants assisting claspless distal extension partial overdentures.Four bilateral mandibular distal extension acrylic models were constructed. Two implants were inserted in the distal extension ridges parallel to each other and perpendicular to the ridges. Based on the implant position, models were categorized into two groups: Group I, mesial implants position and Group II, distal implant position...
January 3, 2017: Journal of Oral Implantology
https://www.readbyqxmd.com/read/28032452/synthesis-of-new-pyrazolo-3-4-d-pyrimidine-derivatives-and-evaluation-of-their-anti-inflammatory-and-anticancer-activities
#11
Heba A Abd El Razik, Mohamed Mroueh, Wissam H Faour, Wassim N Shebaby, Costantine F Daher, Hayam M A Ashour, Hanan M Ragab
The present study reports the synthesis of two series of new purine bioisosteres comprising a pyrazolo[3,4-d]pyrimidine scaffold linked to piperazine moiety through different amide linkages. The newly synthesized compounds were evaluated for anticancer activity against four cell lines (MDA-MB-231, MCF-7, SF-268, B16F-10), and cyclooxygenase (COX-2) protein expression inhibition in lipopolysaccharide (LPS)-activated rat monocytes. The results revealed that most of the synthesized compounds showed moderate to high cytotoxic activity against at least one cell line, with compound 10b being the most active against all used cell lines (IC50 values 5...
December 29, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27972911/fabrication-of-anti-vitiligo-ointment-containing-psoralia-corylifolia-invitro-and-invivo-characterization
#12
I Hussain, N Hussain, A Manan, B Ali, A Rashid
No abstract text is available yet for this article.
November 2016: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27902419/influence-of-tick-and-mammalian-physiological-temperatures-on-borrelia-burgdorferi-biofilms
#13
Shafiq Shaikh, Venkata Arun Timmaraju, Jason P Torres, Kayla M Socarras, Priyanka A S Theophilus, Eva Sapi
The spirochaete bacterium Borrelia burgdorferisensu lato is the aetiologic agent of Lyme disease. Borrelia is transmitted to mammals through tick bite and is adapted to survive at tick and mammalian physiological temperatures. We have previously shown that B. burgdorferi can exist in different morphological forms, including the antibiotic-resistant biofilm form, in vitro and in vivo. B. burgdorferi forms aggregates in ticks as well as in humans, indicating potential of biofilm formation at both 23 and 37 °C...
November 2016: Microbiology
https://www.readbyqxmd.com/read/27882005/can-blood-and-semen-presepsin-levels-in-males-predict-pregnancy-in-couples-undergoing-intra-cytoplasmic-sperm-injection
#14
Ali Ovayolu, Cansev Yilmaz Arslanbuğa, Ismet Gun, Belgin Devranoglu, Arman Ozdemir, Sule Eren Cakar
OBJECTIVE: To determine whether semen and plasma presepsin values measured in men with normozoospermia and oligoasthenospermia undergoing invitro-fertilization would be helpful in predicting ongoing pregnancy and live birth. METHODS: Group-I was defined as patients who had pregnancy after treatment and Group-II comprised those with no pregnancy. Semen and blood presepsin values were subsequently compared between the groups. Parametric comparisons were performed using Student's t-test, and non-parametric comparisons were conducted using the Mann-Whitney U test...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27827458/microrna-126-overexpression-rescues-diabetes-induced-impairment-in-efferocytosis-of-apoptotic-cardiomyocytes
#15
Sahana Suresh Babu, Rajarajan A Thandavarayan, Darukeshwara Joladarashi, Prince Jeyabal, Shashirekha Krishnamurthy, Arvind Bhimaraj, Keith A Youker, Prasanna Krishnamurthy
Efferocytosis, a process of clearance of apoptotic cells by phagocytes, is essential for successful resolution of inflammation and maintenance of tissue homeostasis. Diabetes compromises the function of macrophages leading to adverse inflammatory response during wound healing, myocardial injury, atherosclerosis and autoimmune disorders. However, the effect of diabetes on macrophage-mediated efferocytosis of apoptotic cardiomyocytes (ACM) and the molecular mechanisms involved are not understood so far. In the present study we found that invitro efferocytosis of ACM was impaired in macrophages from db/db (diabetic) mice...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27809851/cyclophilin-a-enhances-macrophage-differentiation-and-lipid-uptake-in-high-glucose-conditions-a-cellular-mechanism-for-accelerated-macro-vascular-disease-in-diabetes-mellitus
#16
Surya Ramachandran, Anandan Vinitha, Cheranellore Chandrasekharan Kartha
BACKGROUND: Vascular disease in diabetes is initiated by monocyte adhesion to vascular endothelium, transmigration and formation of foam cells. Increasing clinical evidence supports a role for the secretory protein, cyclophilin A in diabetic vascular disease. The means by which cyclophilin A contributes to vascular lesion development in diabetes is however largely unknown. METHODS: In this study we investigated using THP1 cells and human monocytes whether cyclophilin A under hyperglycemic conditions, functions in the inflammatory cascade as a chemoattractant and increases lipid uptake by formation of foam cells invitro...
November 3, 2016: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/27790583/comparative-assessment-of-antimicrobial-efficacy-of-different-antibiotic-coated-gutta-percha-cones-on-enterococcus-faecalis-an-invitro-study
#17
Vardhaman Mulchand Jain, Gundabaktha Nagappa Karibasappa, Arun Suresh Dodamani, Prashanth Kumar Vishwakarma, Gaurao Vasant Mali
INTRODUCTION: The major goal of endodontic treatment is to eliminate bacteria from the root canals and prevent re-infection. Enterococcus faecalis (E. faecalis) has been attributed to be the most common organism for the endodontic treatment failures. The choice of endodontic material that have high antimicrobial efficacy can help in decreasing/avoiding growth of micro-organisms and facilitate the success rate of treatment. AIM: The present study was designed with an aim to evaluate the antimicrobial efficacy of antibiotic coated gutta-percha cones on E...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790568/comparative-evaluation-of-the-marginal-sealing-ability-of-two-commercially-available-pit-and-fissure-sealants
#18
Lakshmi Priya Sridhar, Joyson Moses, Bollam Nammalwar Rangeeth, Subramanian Sivakumar
INTRODUCTION: The occlusal surfaces of the teeth usually have pits and fissures which provide a good environment for demineralization with minimal salivary access and make them caries prone. The success of pit and fissure sealing materials is highly dependent on the marginal sealing ability of the fissures. AIM: The present study was carried out to evaluate and compare the marginal sealing ability of two commercially available pit and fissure sealants. MATERIALS AND METHODS: A total of 50 human premolar teeth were used in this invitro study...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790459/estimation-of-antimicrobial-properties-of-aqueous-and-alcoholic-extracts-of-salvadora-persica-miswak-on-oral-microbial-pathogens-an-invitro-study
#19
Salman Siddeeqh, Amrita Parida, Maji Jose, Vidya Pai
INTRODUCTION: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. AIM: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. MATERIALS AND METHODS: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790457/in-vitro-comparison-of-generic-and-branded-preparations-of-amoxicillin-with-potassium-clavulanate
#20
Priyanka Pathak, Jayshree Dawane
INTRODUCTION: The Medical Council of India (MCI) has guidelines for physicians to prescribe drugs with generic names. But physicians and patients might have prejudices against generic drug substitution or concerns regarding quality and generics effectiveness. If the drugs are ineffective, they can result in adverse clinical outcomes such as treatment failure. According to WHO, β-lactams are the most common substandard drugs to be produced amongst antibiotics. AIM: To evaluate and compare invitro efficacy of generic and branded preparations of Amoxicillin with Potassium Clavulanate...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
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