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Harsha Lad, Deepak Bhatnagar
The present study was aimed to determine the therapeutic effects of Swertia chirayita leaves against oxidative and inflammatory injuries in Freund's complete adjuvant (FCA) induced arthritic rats. The extract was evaluated for its phytoconstituents and various invitro antioxidant properties followed by its in vivo effects. The hydroethanolic extract of S. chirayita leaves (SCE) was orally administered (200 mg/kg body weight, per day, p.o.) and the effect on the liver lipid peroxidation (LPO), antioxidant status, protein carbonyl formation along with the histopathology of liver were evaluated after induction of adjuvant arthritis...
October 14, 2016: Inflammopharmacology
Jian Yang, Azadeh Bahreman, Geert Daudey, Jeroen Bussmann, René C L Olsthoorn, Alexander Kros
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low delivery efficiency due to limited endosomal escape and/or degradation in lysosomes. Here, we report a new method for direct drug delivery into the cytosol of live cells in vitro and invivo utilizing targeted membrane fusion between liposomes and live cells. A pair of complementary coiled-coil lipopeptides was embedded in the lipid bilayer of liposomes and cell membranes respectively, resulting in targeted membrane fusion with concomitant release of liposome encapsulated cargo including fluorescent dyes and the cytotoxic drug doxorubicin...
September 28, 2016: ACS Central Science
Ferah Rehman, Gyanendra Kumar, Mridula Goswami, Jatinder Kaur Dhillon
Aim: The aim of this invitro study is to evaluate the shear bond strength of reattached fractured incisor fragments using Er,Cr:YSGG laser and conventional acid etching without additional tooth preparation. Materials and methods: Forty extracted human teeth were divided in two groups of 20 each (Groups A and B). In Group A, fractured surface was treated by an Er, Cr: YSGG laser system (Waterlase MD, Biolase Technology Inc., San Clemente, CA, USA) operating at a wavelength of 2,780 nm and frequency of 20 Hz...
June 29, 2016: Laser Therapy
Madhusudana Pulaganti, Anuradha C M, Chitta Suresh Kumar
Pseudomonas aeruginosa (P.aeruginosa) is an opportunistic microorganism causing diseases both in animals and humans. In case of human pathology, the role of P.aeruginosa is one of the major concerns in intensive care septicemia. Presently the drug resistance strains of P.aeruginosa are arising mainly by developing multiple mechanisms due to its natural and acquired resistance to many of the antimicrobial agents commonly used in clinical practice. As a result, there is a direneed to invent new drugs so that they may restrict the outbreak of multidrug resistant strains...
August 15, 2016: Journal of Biomolecular Structure & Dynamics
Niyati H Mudliar, Biswajit Sadhu, Aafrin M Pettiwala, Prabhat K Singh
Recently, Auramine O (AuO) has been projected as a fluorescent fibril sensor, and it has been claimed that AuO has an advantage over the most extensively utilized fibril marker, Thioflavin-T (ThT), owing to the presence of an additional large red-shifted emission band for AuO, which was observed exclusively for AuO in the presence of fibrillar media and not in protein or buffer media. As fibrils are very rich in β-sheet structure, a fibril sensor should be more specific toward the β-sheet structure so as to produce a large contrast between the fibril form and native protein form, for efficient detection and in vitro mechanistic studies of fibrillation...
October 13, 2016: Journal of Physical Chemistry. B
Mini Saluja, Harpreet Singh Grover, Pankaj Choudhary
INTRODUCTION: Dentinal hypersensitivity is one of the most common problem, encountered in dental practice but has least predictable treatment outcome. The advent of lasers in dentistry has provided an additional therapeutic option for treating dentinal hypersensitivity. Although various lasers have been tried over a period of time to treat dentinal hypersensitivity, but still the doubt persist as to which laser leads to maximum dentinal tubular occlusion and is most suitable with minimal hazardous effects...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Labib Mohamed Labib, Sameh Mahmoud Nabih, Kusai Baroudi
INTRODUCTION: The effect of nanoleakage on the integrity of resin-dentin bond has been in interest for long-term adhesion. AIM: This study evaluated the nanoleakage in premolar teeth restored with low shrinkable resin composite. MATERIALS AND METHODS: A total of 40 human premolars were used for nanoleakage evaluation in this study. Each group was divided into four equal groups; Group A: using silorane with its adhesive system. Group B: using silorane with G-bond...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Andréa P Carnizello, Marília I F Barbosa, Monize Martins, Natália H Ferreira, Pollyanna F Oliveira, Geórgia M Magalhães, Alzir A Batista, Denise C Tavares
This study performed in vitro and in vivo biological assays of the ruthenium (II) compound ct-[RuCl(CO)(dppb)(bipy)]PF6 (where, dppb=1,4-bis(diphenylphosphine)butane and bipy=2,2'-bipyridine). The cytotoxic activity of this compound was evaluated against different tumor cell lines (HeLa, human cervical adenocarcinoma; MCF7, human breast adenocarcinoma; MO59J, human glioblastoma; HepG2, hepatocellular carcinoma and B16F10, murine melanoma) and healthy cell line (V79, Chinese hamster lung fibroblasts), by XTT (sodium 2,3'-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-3,4-tetrazolium-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) method...
August 25, 2016: Journal of Inorganic Biochemistry
Viswanath Ragupathy, Wang Xue, Ji Tan, Krishnakumar Devadas, Yamei Gao, Indira Hewlett
In human immunodeficiency virus type 1 (HIV-1)-infected women, oral or injectable progesterone containing contraceptive pills may enhance HIV-1 acquisition in vivo, and the mechanism by which this occurs is not fully understood. In developing countries, Herpes simplex virus type-2 (HSV-2) co-infection has been shown to be a risk for increase of HIV-1 acquisition and, if co-infected women use progesterone pills, infections may increase several fold. In this study, we used an in vitro cell culture system to study the effects of progesterone on HIV-1 replication and to explore the molecular mechanism of progesterone effects on infected cells...
October 2016: Journal of Molecular Endocrinology
Ajay K Chaudhary, Shruti Chaudhary, Kanjaksha Ghosh, A Nadkarni
Controlled remodeling of the extracellular matrix (ECM) is essential for cell growth, invasion and metastasis. Matrix metalloproteinases (MMPs) are a family of secreted, zincdependent endopeptidases capable of degradation of ECM components. The expression and activity of MMPs in a variety of human cancers have been intensively studied. They play important roles at different steps of malignant tumor formation and have central significance in embryogenesis, tissue remodeling, inflammation, angiogenesis and metastasis...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Krupa Shah, Sonia Parikh, Rakesh Rawal
Chronic myeloid leukaemia (CML) is a clonal myeloproliferative hematopoietic stem cell disorder. Deregulated BCRABL fusion tyrosine kinase activity is the main cause of CML disease pathogenesis, making BCRABL an ideal target for inhibition. Current tyrosine kinase inhibitors (TKIs) designed to inhibit BCRABL oncoprotein activity, have completely transformed the prognosis of CML. Interruption of TKI treatment leads to minimal residual disease reside (MRD), thought to reside in TKIinsensitive leukaemia stem cells which remain a potential reservoir for disease relapse...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Naveen Reddy Vootla, Sarat Chandra Barla, Vhc Kumar, Hemchand Surapaneni, Srilatha Balusu, Swetha Kalyanam
INTRODUCTION: Studies on stress distribution around screw retained implants in different bone densities are limited. In clinical situations crowns of different heights are placed on the implants and the effect of varying crown implant ratio on the bone is not understood properly. AIM: To evaluate and compare the stress distribution in different screw retained implants for different crown-implant ratios in different bone densities under various occlusal loads using three dimensional finite element analyses...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
Bastin Cherian, Paras Mull Gehlot, Mysore Krishnaswamy Manjunath
INTRODUCTION: Elimination of microorganisms from infected root canals is a complicated task. Numerous measures have been described to reduce the microbial load in the root canal system, including the use of various instrumentation techniques, irrigation regimens and intracanal medicaments. The drawbacks of few commonly used irrigants include toxic and harmful side effects, microbial resistance to antimicrobial agents and staining. Hence there is a need for alternative agents which are nontoxic, effective and safe...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
Kaliyan Barathikannan, Babu Venkatadri, Ameer Khusro, Naif Abdullah Al-Dhabi, Paul Agastian, Mariadhas Valan Arasu, Han Sung Choi, Young Ock Kim
BACKGROUND: The medical application of pomegranate fruits and its peel is attracted human beings. The aim of the present study was to evaluate the in vitro α-Glucosidase inhibition, antimicrobial, antioxidant property and in vivo anti-hyperglycemic activity of Punica granatum (pomegranate) fruit peel extract using Caenorhabditis elegans. METHODS: Various invitro antioxidant activity of fruit peel extracts was determined by standard protocol. Antibacterial and antifungal activities were determined using disc diffusion and microdilution method respectively...
2016: BMC Complementary and Alternative Medicine
Leila Sadeghi, Farzeen Tanwir, Vahid Yousefi Babadi
Medicago sativa Linn. or alfalfa (Leguminosae) has been used traditionally as an effective cure for CNS, heart and metabolic disorders and digestive aids. Alfalfa is a resistant plant against stress due to small antioxidant molecules and enzymes. Our previous work demonstrated that 100 μg/ml of 50 nm size Fe2O3-NP causes harsh oxidative stress in HepG2 cells and 100 mg/kg of same nanoparticle causes extreme damage in rat's liver. Therefore it can be used as a useful model for invivo and invitro studies of oxidative stress...
July 18, 2016: Regulatory Toxicology and Pharmacology: RTP
Attiguppe Ramashetty Prabhakar, Deepti V Balehosur, Nadig Basappa
INTRODUCTION: Atraumatic restorative treatment is a minimal intervention approach which involves manual removal of caries followed by restoration using adhesive restorative material. Due to incomplete manual caries excavation, there is a high chance of secondary caries under the restoration. Hence, many antibacterial agents have been incorporated in cement to enhance their antibacterial effect. Propolis is one of the natural medicines that has highlighted application in dentistry. AIM: The current study evaluated the shear bond strength and fluoride release of Glass Ionomer Cement (GIC) combined with 1% Ethanolic Extract of Propolis (EEP)...
May 2016: Journal of Clinical and Diagnostic Research: JCDR
Preethi S, Mary Saral A
Polysaccharides were extracted from the dried fruiting bodies of Pithecellobium dulce with 20% ethanol by microwave-assisted extraction. The polysaccharides were isolated by ion exchange chromatography and afford three water-soluble polysaccharides PDP-1, PDP-2, and PDP-3. These isolated compounds were subjected to acid hydrolysis, methylation, IR and GC-MS for its compositional analysis and revealed that all the three fractions are heteropolysaccharides. PDP-1 was found to be composed of xylose, mannose, galactose and Rhamnose...
July 12, 2016: International Journal of Biological Macromolecules
N S Remya, Prabha D Nair
Fetal-derived mesenchymal stem cells especially human umbilical cord matrix mesenchymal stem cells (hUCMSCs), with their ease of availability, pluripotency, and high expansion potential have emerged as an alternative solution for stem cell based cartilage therapies. An attempt to elucidate the effect of dynamic mechanical compression in modulating the chondrogenic differentiation of hUCMSCs is done in this study to add on to the knowledge of optimizing chondrogenic signals necessary for the effective differentiation of these stem cells and subsequent integration to the surrounding tissues...
October 2016: Journal of Biomedical Materials Research. Part A
Sonia Tiwari, Mallikarjuna Kenchappa, Deepak Bhayya, Shilpi Gupta, Sudhanshu Saxena, Saurabh Satyarth, Aishwarya Singh, Manoj Gupta
INTRODUCTION: The cariostatic property of glass ionomer cement (GIC) stems from its ability to release fluoride into the oral environment. Recently, zirconia reinforced GIC has been launched which promises the protective benefits of glass ionomer while completely eliminating the hazard of mercury. AIM: To evaluate invitro antibacterial activity and fluoride release from two conventional glass ionomer cements (GC II and GC IX), compomer (Compoglass) and a zirconia reinforced glass ionomer cement (Zirconomer)...
April 2016: Journal of Clinical and Diagnostic Research: JCDR
Poornima Jose, Kavitha Sanjeev, Mahalaxmi Sekar
INTRODUCTION: Mechanical performance of dentine is of major significance for the overall function of the teeth. Remineralization of carious dentine is the ultimate goal in re-establishing the functionality of the affected tissue so as to regain and maintain the mechanical properties of dentine. Functional remineralization of the affected dentin involves stabilization of both inorganic and organic component, but Caesin Phosphopeptide Amorphous Calcium Flurophosphate (CPP-ACFP) stabilizes only inorganic content...
April 2016: Journal of Clinical and Diagnostic Research: JCDR
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