keyword
MENU ▼
Read by QxMD icon Read
search

Invitro

keyword
https://www.readbyqxmd.com/read/29103204/invitro-evaluation-of-torin2-and-2-6-dihydroxyacetophenone-in-colorectal-cancer-therapy
#1
Ankita Awasthi, Pharvendra Kumar, Chittur V Srikanth, Shakti Sahi, Rekha Puria
Colorectal cancer (CRC) is one of the most prevalent cancers diagnosed worldwide. Despite recent advances, resistance to cytotoxic and targeted therapy remains one of the greatest challenges in long-term management of colorectal cancer therapy. Recently established role of mTOR signaling in proliferation of CRC has incited for evaluation of mTOR kinase specific inhibitors in CRC therapy. Second generation mTOR kinase inhibitors including Torin2 has demonstrated efficient anticancer properties against variety of cancers and are in various stages of drug development...
November 4, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29096755/ziziphus-jujube-a-review-study-of-its-anticancer-effects-in-various-tumor-models-invitro-and-invivo
#2
S Ebrahimi, H Mollaei, R Hoshyar
The growing rate of cancer incidences and inefficiency of current therapies have made scientists to find novel agents such as medicinal plants. Ziziphus jujube is one of these plants whose pharmaceutical effects have been studied for a long time. Recent investigations have proved that bioactive compounds of Ziziphus jujube including triterpenic acids, flavonoids, cerebrosides, phenolic acids, α tocopherol, β carotene, and polysaccharides have anti-proliferative effects on several cancerous cell lines and animals...
October 31, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29092676/characterization-of-domain-specific-interaction-of-synthesized-dye-with-serum-proteins-by-spectroscopic-and-docking-approaches-along-with-determination-of-invitro-cytotoxicity-and-antiviral-activity
#3
Suparna Rudra, Somnath Dasmandal, Chiranjit Patra, Biman Kumar Patel, Suvendu Paul, Ambikesh Mahapatra
The interaction between a synthesized dye with proteins, bovine and human serum albumin (BSA, HSA respectively) under physiological conditions has been characterized in details, by means of steady state and time resolved fluorescence, UV-Vis absorption and circular dichroism (CD) techniques. An extensive time-resolved fluorescence spectroscopic characterization of the quenching process has been undertaken in conjugation with temperature-dependent fluorescence quenching studies to divulge the actual quenching mechanism...
November 1, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29059021/self-assembled-supramolecular-thermoreversible-%C3%AE-cyclodextrin-ethylene-glycol-injectable-hydrogels-with-difunctional-pluronic-%C3%A2-127-as-controlled-delivery-depot-of-curcumin-development-characterization-and-in-vitro-evaluation
#4
Samiullah Khan, Muhammad Usman Minhas, Mahmood Ahmad, Mohammad Sohail
This study aimed to design a novel thermoreversible compactself-assembled host macromolecule based on β-cyclodextrin and low molecular weight ethylene glycol with a difunctional guest molecule i.e. poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) tri-block co-polymer (Pluronic(®) 127) through host-guest inclusion complexation in aqueous media using cold method. The injectable thermoreversible self-assembled supramolecular hydrogel was developed to encapsulate hydrophobic curcumin as anti-malignant agent andfor controlled delivery to systemic circulation after invivo application through subcutaneous route...
October 23, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29040498/genome-stability-of-bovine-in-vivo-conceived-cleavage-stage-embryos-is-higher-compared-to-in-vitro-produced-embryos
#5
Olga Tšuiko, Maaike Catteeuw, Masoud Zamani Esteki, Aspasia Destouni, Osvaldo Bogado Pascottini, Urban Besenfelder, Vitezslav Havlicek, Katrien Smits, Ants Kurg, Andres Salumets, Thomas D'Hooghe, Thierry Voet, Ann Van Soom, Joris Robert Vermeesch
STUDY QUESTION: Is the rate and nature of chromosome instability (CIN) similar between bovine in vivo-derived and in vitro-cultured cleavage-stage embryos? SUMMARY ANSWER: There is a major difference regarding chromosome stability of in vivo-derived and in vitro-cultured embryos, as CIN is significantly lower in in vivo-derived cleavage-stage embryos compared to in vitro-cultured embryos. WHAT IS KNOWN ALREADY: CIN is common during in vitro embryogenesis and is associated with early embryonic loss in humans, but the stability of in vivo-conceived cleavage-stage embryos remains largely unknown...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29028222/role-of-rbp2-induced-er-and-igf1r-erbb-signaling-in-tamoxifen-resistance-in-breast-cancer
#6
Hee-Joo Choi, Hyeong-Seok Joo, Hee-Young Won, Kyueng-Whan Min, Hyung-Yong Kim, Taekwon Son, Young-Ha Oh, Jeong-Yeon Lee, Gu Kong
Background: Despite the benefit of endocrine therapy, acquired resistance during or after treatment still remains a major challenge in estrogen receptor (ER)-positive breast cancer. We investigated the potential role of histone demethylase retinoblastoma-binding protein 2 (RBP2) in endocrine therapy resistance of breast cancer. Methods: Survival of breast cancer patients according to RBP2 expression was analyzed in three different breast cancer cohorts including METABRIC (n = 1980) and KM plotter (n = 1764)...
April 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28987248/the-effect-of-mipaste-plus-and-reminpro-on-incipient-caries-using-diagnodent-and-sem-an-invitro-study
#7
Bassir Leila, Saeid Nemati, Hajalizadeh Neda, Mashallah Khanehmasjedi
BACKGROUND: The remineralization of early caries lesion has an effective role on decreasing caries. For initial remineralization of caries lesion, using Compounds of Casein phosphopeptides (CPP) in different studies has been proposed. REMINPRO including Fluoride, Xylitol and Calcium phosphate has just been offered in the market. This study aims to investigate the efficacy of Reminpro and MI paste plus in terms of remineralization of early enamel lesions. MATERIALS AND METHODS: In 15 samples of healthy enamels of the maxillary first premolar teeth and 15 samples of Primary Mandibular First Molar under the effect of demineralized gel, artificial caries has been created...
2017: Journal of the National Medical Association
https://www.readbyqxmd.com/read/28977352/the-optimized-hplc-method-for-quantitative-analysis-of-phenylethyl-resorcinol-loaded-in-the-novel-vesicle-carriers-and-permeated-in-in-vitro-skin-permeation-study
#8
Tunyaluk Limsuwan, Prapaporn Boonme, Thanaporn Amnuaikit
The high performance liquid chromatography (HPLC) was used for quantitative determination of phenylethyl resorcinol (PR) which was loaded in the novel vesicle carriers including ethosome, invasome and transfersome formulations, and permeated into pig skin membrane and receptor fluid for skin permeation study. The reverse-phase chromatography was carried out with a C18 column (150 × 4.6 mm2, 5 μm, HypersilTM, Thermo Fisher Scientific Inc, USA) with the column temperature at 25°C. A mixture of acetonitrile-methanol-Milli-Q water in the ratio of 40:20:40%, v/v/v was used as a mobile phase by maintaining the flow rate at 0...
November 1, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28974831/comparing-the-effects-of-krebs-plus-verapamil-solution-on-endothelial-function-of-harvested-human-greater-saphenous-vein-with-heparinized-blood-an-invitro-study
#9
Mitra Shokri, Reza Moradpour, Majid Malekzade Shafaroudi, Nourollah Rezaei, Shervin Ziabakhsh Tabary
INTRODUCTION: Integrity of the great saphenous vein (GSV) endothelium is the most important key element for long-term patency rate of grafts in coronary artery bypass graft (CABG). Storage solutions play an important role in maintaining viability of vein endothelium. Diminished nitric oxide (NO) because of endothelial dysfunction may facilitate vascular inflammation and formation of atherosclerotic plaque. AIM: So, we decided to find a reasonable alternative preservative solution instead of heparinized blood (HB) by measuring NO concentration with Griess assay...
June 2017: Medical Archives
https://www.readbyqxmd.com/read/28962527/hepatic-amiodarone-lipotoxicity-is-ameliorated-by-genetic-and-pharmacological-inhibition-of-endoplasmatic-reticulum-stress
#10
Noam Erez, Einav Hubel, Roy Avraham, Raya Cohen, Sigal Fishman, Heike Bantel, Michael Manns, Boaz Tirosh, Isabel Zvibel, Oren Shibolet
Amiodarone is a commonly used antiarrhythmic drug and can cause liver steatosis. We investigated the role of endoplasmic reticulum (ER) stress/unfolded protein response in the pathogenesis of amiodarone-induced steatosis. Amiodarone-induced liver injury was obtained by 1 intraperitoneal injection to wild-type (WT) or C/EBP homologous protein knock-out mice (Ddit3-/-). Amiodarone directly reduced intracellular ATP and Ca2+ in hepatocytes invitro, inducing ER stress and lipid accumulation. In vivo, amiodarone-driven liver damage and lipid accumulation was accompanied by activation of ER stress/unfolded protein response, as demonstrated by up-regulation of genes encoding key ER stress mediators and by phosphorylation of eIF2α...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28962522/characterization-of-triptolide-induced-hepatotoxicity-by-imaging-and-transcriptomics-in-a-novel-zebrafish-model
#11
Adriaan D Bastiaan Vliegenthart, Chunmin Wei, Charlotte Buckley, Cécile Berends, Carmelita M J de Potter, Sarah Schneemann, Jorge Del Pozo, Carl Tucker, John J Mullins, David J Webb, James W Dear
Triptolide is a vine extract used in traditional Chinese medicines and associated with hepatotoxicity. In vitro data suggest that inhibition of RNA synthesis may be the mechanism of toxicity. For studying drug-induced liver injury the zebrafish has experimental, practical and financial advantages compared with rodents. The aim of this study was to explore the mechanism of triptolide toxicity using zebrafish as the model system. The effect of triptolide exposure on zebrafish larvae was determined with regard to mortality, histology, expression of liver specific microRNA-122 and liver volume...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28962518/from-the-cover-an-animal-free-in-vitro-three-dimensional-testicular-cell-coculture-model-for-evaluating-male-reproductive-toxicants
#12
Lei Yin, Hongye Wei, Shenxuan Liang, Xiaozhong Yu
Primary testicular cell coculture model has been used to evaluate testicular abnormalities during development, and was able to identify the testicular toxicity of phthalates. However, the primary testicular cell coculture model has disadvantages in employing animals for the isolation of testicular cells, and the complicated isolation procedure leads to inconsistent results. We developed an invitro testicular coculture model from rodent testicular cell lines, including spermatogonial cells, Sertoli cells, and Leydig cells with specified cell density and extracellular matrix (ECM) composition...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28957507/accelerated-evolution-and-functional-divergence-of-the-dim-light-visual-pigment-accompanies-cichlid-colonization-of-central-america
#13
Frances E Hauser, Katriina L Ilves, Ryan K Schott, Gianni M Castiglione, Hernán López-Fernández, Belinda S W Chang
Cichlids encompass one of the most diverse groups of fishes in South and Central America, and show extensive variation in life history, morphology, and colouration. While studies of visual system evolution in cichlids have focussed largely on the African rift lake species flocks, Neotropical cichlids offer a unique opportunity to investigate visual system evolution at broader temporal and geographic scales. South American cichlid colonization of Central America has likely promoted accelerated rates of morphological evolution in Central American lineages as they encountered reduced competition, renewed ecological opportunity, and novel aquatic habitats...
October 1, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28936163/quercetin-declines-apoptosis-ameliorates-mitochondrial-function-and-improves-retinal-ganglion-cell-survival-and-function-in-in-vivo-model-of-glaucoma-in-rat-and-retinal-ganglion-cell-culture-in-vitro
#14
Feng-Juan Gao, Sheng-Hai Zhang, Ping Xu, Bo-Qi Yang, Rong Zhang, Yun Cheng, Xu-Jiao Zhou, Wan-Jing Huang, Min Wang, Jun-Yi Chen, Xing-Huai Sun, Ji-Hong Wu
Glaucoma is a progressive neuropathy characterized by the loss of retinal ganglion cells (RGCs). Strategies that delay or halt RGC loss have been recognized as potentially beneficial for rescuing vision in glaucoma patients. Quercetin (Qcn) is a natural and important dietary flavonoid compound, widely distributed in fruits and vegetables. Mounting evidence suggests that Qcn has numerous neuroprotective effects. However, whether Qcn exerts neuroprotective effects on RGC in glaucoma is poorly understood. In this study, we investigated the protective effect of Qcn against RGC damage in a rat chronic ocular hypertension (COHT) model invivo and hypoxia-induced primary cultured RGC damage in vitro, and we further explored the underlying neuroprotective mechanisms...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28934378/investigating-difficult-nodes-in-the-placental-mammal-tree-with-expanded-taxon-sampling-and-thousands-of-ultraconserved-elements
#15
Jacob A Esselstyn, Carl H Oliveros, Mark T Swanson, Brant C Faircloth
The phylogeny of eutherian mammals contains some of the most recalcitrant nodes in the tetrapod tree of life. We combined comprehensive taxon and character sampling to explore three of the most debated interordinal relationships among placental mammals. We performed in silico extraction of ultraconserved element loci from 72 published genomes and invitro enrichment and sequencing of ultraconserved elements from 28 additional mammals, resulting in alignments of 3,787 loci. We analyzed these data using concatenated and multispecies coalescent phylogenetic approaches, topological tests, and exploration of support among individual loci to identify the root of Eutheria and the sister groups of tree shrews (Scandentia) and horses (Perissodactyla)...
September 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28903496/editor-s-highlight-development-of-novel-neural-embryonic-stem-celltests-for-high-throughput-screening-of-embryotoxic-chemicals
#16
Kumiko Kobayashi, Noriyuki Suzuki, Kiyoshi Higashi, Akane Muroi, Florian Le Coz, Hirohisa Nagahori, Koichi Saito
There is a great demand for appropriate alternative methods to rapidly evaluate the developmental and reproductive toxicity of a wide variety of chemicals. We used the differentiation of mouse embryonic stem cells (mESCs) into cardiomyocytes as a basis for establishing a rapid and highly reproducible invitro embryotoxicity test known as the Hand1-Luc Embryonic Stem Cell Test (Hand1-Luc EST). In this study, we developed novel neural-Luc ESTs using two marker genes for neural development, tubulin beta-3 (Tubb3) and Reelin (Reln), and evaluated the capacity of these tests to predict developmental toxicity...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903487/from-the-cover-arsenic-induces-hippocampal-neuronal-apoptosis-and-cognitive-impairments-via-an-up-regulated-bmp2-smad-dependent-reduced-bdnf-trkb-signaling-in-rats
#17
Rukmani Pandey, Vipin Rai, Juhi Mishra, Kapil Mandrah, Somendu Kumar Roy, Sanghamitra Bandyopadhyay
Arsenic promotes hippocampal neuronal damage inducing cognitive impairments. However, mechanism arbitrating arsenic-mediated cognitive deficits remains less-known. Here, we identified that chronic exposure to environmentally relevant doses of arsenic increased apoptosis, characterized by caspase-3 activation, poly(ADP-ribose) polymerase cleavage and Terminal deoxynucleotidyl transferase dUTP nick-end labeling of rat hippocampal neurons, marked by NeuN. Investigating apoptotic mechanism through invivo and invitro studies revealed that arsenic promoted bone morphogenetic protein-2 (BMP2) expression, supported by increased BMP-receptor2 (BMPR2) and p-Smad1/5 in hippocampal neurons...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903485/editor-s-highlight-mechanistic-toxicity-tests-based-on-an-adverse-outcome-pathway-network-for-hepatic-steatosis
#18
Michelle M Angrish, Charlene A McQueen, Elaine Cohen-Hubal, Maribel Bruno, Yue Ge, Brian N Chorley
Risk assessors use liver endpoints in rodent toxicology studies to assess the safety of chemical exposures. Yet, rodent endpoints may not accurately reflect human responses. For this reason and others, human-based invitro models are being developed and anchored to adverse outcome pathways to better predict adverse human health outcomes. Here, a networked adverse outcome pathway-guided selection of biology-based assays for lipid uptake, lipid efflux, fatty acid oxidation, and lipid accumulation were developed...
September 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28903484/novel-role-of-fbxw7-circular-rna-in-repressing-glioma-tumorigenesis
#19
Yibing Yang, Xinya Gao, Maolei Zhang, Sheng Yan, Chengjun Sun, Feizhe Xiao, Nunu Huang, Xuesong Yang, Kun Zhao, Huangkai Zhou, Suyun Huang, Bo Xie, Nu Zhang
Background: Circular RNAs (circRNAs) are RNA transcripts that are widespread in the eukaryotic genome. Recent evidence indicates that circRNAs play important roles in tissue development, gene regulation, and carcinogenesis. However, whether circRNAs encode functional proteins remains elusive, although translation of several circRNAs was recently reported. Methods: CircRNA deep sequencing was performed by using 10 pathologically diagnosed glioblastoma samples and their paired adjacent normal brain tissues...
March 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28866193/injectable-natural-polymer-compound-for-tissue-engineering-of-intervertebral-disc-in-vitro-study
#20
Masoud Ghorbani, Jafar Ai, Mohammad Reza Nourani, Mahmoud Azami, Batool Hashemi Beni, Shiva Asadpour, Sima Bordbar
Intervertebral disc degeneration is recognized to be the leading cause for chronic low-back pain. Injectable hydrogel is one of the great interests for tissue engineering and cell encapsulation specially for intervertebral (IVD) affecting rate of regeneration success, in this study we assessed viscoelastic properties of a Chitosan-β glycerophosphate-hyaluronic acid, Chondroitin-6-sulfate, type 2 of Collagen, gelatin, fibroin silk (Ch-β-GP-HA-CS-Col-Ge-FS) hydrogel which was named as NP hydrogel that is natural extracellular matrix of IVD...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
keyword
keyword
66835
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"