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https://www.readbyqxmd.com/read/29777811/electrospun-chitosan-polycaprolactone-hyaluronic-acid-bilayered-scaffold-for-potential-wound-healing-applications
#1
Amit Chanda, Jaideep Adhikari, Aritri Ghosh, Sougata Roy Chowdhury, Sabu Thomas, Pallab Datta, Prosenjit Saha
Fabrication of mechanically stable, biocompatible bilayered polymeric scaffold consisting of chitosan(CS)/polycaprolactone(PCL) and hyaluronic acid(HA) using less toxic solvent system is presented in this study. Electrospinning technique to make the scaffold was used followed by morphological, physiochemical and mechanical characterizations. Average fiber diameter of CS/PCL-HA bilayered scaffold was found 362.2 ± 236 nm which is in the range of collagen fiber found in the extracellular matrices. Enhanced swelling, degradation, hydrophilicity and water vapour transmission rate were found for the bilayered scaffold compared to that of the PCL and CS-PCL scaffolds...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29773109/recombinant-%C3%AB-defensin-126-promotes-bull-sperm-binding-to-bovine-oviductal-epithelia
#2
A Lyons, F Narciandi, E Donnellan, J Romero-Aguirregomezcorta, C O' Farrelly, P Lonergan, K G Meade, S Fair
Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells invitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126...
May 18, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29728233/multifunctional-chitosan-capped-gold-nanoparticles-for-enhanced-cancer-chemo-radiotherapy-an-invitro-study
#3
Mohamed Mahmoud Fathy, Fayrouz S Mohamed, NihalSaad Elbialy, Wael M Elshemey
Over the last decade, chemo-radiotherapy represented a well-established paradigm for cancer treatment. Developing new strategies to promote the therapeutic efficacy while reducing toxic side effects of chemo-radiotherapy is a main research objective in cancer therapy. A promising new oncological strategy for enhancing chemo-radiotherapy against cancer involves the utilization of multifunctional nanoparticles (nanocarriers and radiosensitizers). In this work, Chitosan-Capped Gold Nanoparticles (CS-GNPs) were synthesized and loaded with an anticancer agent, Doxorubicin (CS-GNPs-DOX)...
April 2018: Physica Medica: PM
https://www.readbyqxmd.com/read/29720337/insulin-promotes-preantral-follicle-growth-and-antrum-formation-through-temporal-expression-of-genes-regulating-steroidogenesis-and-water-transport-in-the-cat
#4
Chommanart Thongkittidilok, Ram Pratap Singh, Pierre Comizzoli, David Wildt, Nucharin Songsasen
The aims of the present study were to determine the effects of insulin, invitro, on: (1) the viability and growth of domestic cat ovarian follicles; (2) mRNA expression of genes regulating steroidogenesis (cytochrome P450 family 17 subfamily, A polypeptide 1 (Cyp17a1), cytochrome P450 family 19 subfamily, A polypeptide 1 (Cyp19a1) and steroidogenic acute regulatory protein (Star)) and water transport (aquaporins (AQPs) Aqp1, Aqp3, Aqp7, Aqp9); and (3) steroid production (17β-oestradiol (E2), progesterone (P4), androstenedione (A4))...
May 3, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29685182/cotreatment-with-repsox-and-lbh589-improves-the-invitro-developmental-competence-of-porcine-somatic-cell-nuclear-transfer-embryos
#5
Zhao-Bo Luo, Long Jin, Qing Guo, Jun-Xia Wang, Xiao-Xu Xing, Mei-Fu Xuan, Qi-Rong Luo, Guang-Lei Zhang, Xi-Jun Yin, Jin-Dan Kang
Accumulating evidence suggests that aberrant epigenetic reprogramming and low pluripotency of donor nuclei lead to abnormal development of cloned embryos and underlie the inefficiency of mammalian somatic cell nuclear transfer (SCNT). The present study demonstrates that treatment with the small molecule RepSox alone upregulates the expression of pluripotency-related genes in porcine SCNT embryos. Treatment with the histone deacetylase inhibitor LBH589 significantly increased the blastocyst formation rate, whereas treatment with RepSox did not...
April 24, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29660245/1-2-3-triazole-tagged-3h-pyrano-2-3-d-pyrimidine-6-carboxylate-derivatives-synthesis-invitro-anticancer-activity-molecular-docking-and-dna-interaction-studies
#6
Sathish Kumar Boda, Vasantha Pishka, P V Anantha Lakshmi, Srinivas Chinde, Paramjit Grover
A series of novel ethyl 2,7-dimethyl-4-oxo-3-((1-phenyl-1H-1,2,3-triazol-4-yl)methyl)-4,5-dihydro-3H-pyrano[2,3-d]pyrimidine-6-carboxylate derivatives 7(a-m) were efficiently synthesized employing click chemistry approach and evaluated for in vitro anti cancer activity against four tumor cell lines: A549 (human lung adenocarcenoma cell line), HepG2 (Human hematoma), MCF-7 (Human breast adenocarcinoma), and SKOV3 (human ovarian carcinoma cell line). Among the compounds tested, the compounds 7a, 7b, 7f, 7l and 7m have shown potential and selective activity against human lung adenocarcenoma cell line (A549) with IC50 ranging from 0...
April 16, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29627568/the-role-of-xbp1s-in-the-metastasis-and-prognosis-of-hepatocellular-carcinoma
#7
Sudong Wu, Rui Du, Chen Gao, Jingbo Kang, Juyi Wen, Tao Sun
Tumor metastasis and recurrence are the primary contributors to poor prognosis in patients with hepatocellular carcinoma (HCC). The epithelial-mesenchymal transition (EMT) of tumor cells is the predominant mechanism of HCC progression. XBP1s is a newly discovered molecule involved in the endoplasmic reticulum (ER) stressresponse, which is an adaptive response and defense mechanism in cells that enablessurvival under adverse conditions. Abnormally high XBP1sexpression has been found in tumor cells, but the role of XBP1sin HCC progression remains unclear...
April 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29617836/met-receptor-tyrosine-kinase-and-chemoprevention-of-oral-cancer
#8
Pierre Saintigny, William N William, Jean-Philippe Foy, Vassiliki Papadimitrakopoulou, Wenhua Lang, Li Zhang, You Hong Fan, Lei Feng, Edward S Kim, Adel K El-Naggar, J Jack Lee, Li Mao, Waun Ki Hong, Mark W Lingen, Scott M Lippman
Background: We have previously shown that gene expression profiles of oral leukoplakia (OL) may improve the prediction of oral cancer (OC) risk. To identify new targets for prevention, we performed a systematic survey of transcripts associated with an increased risk of oral cancer and overexpressed in OC vs normal mucosa (NM). Methods: We used gene expression profiles of 86 patients with OL and available outcomes from a chemoprevention trial of OC and NM. MET expression was evaluated using immunohistochemistry in 120 OL patients, and its association with OC development was tested in multivariable analysis...
March 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/29608572/development-and-properties-of-duplex-mgf2-pcl-coatings-on-biodegradable-magnesium-alloy-for-biomedical-applications
#9
Preeti Makkar, Hoe Jin Kang, Andrew R Padalhin, Ihho Park, Byoung-Gi Moon, Byong Taek Lee
The present work addresses the performance of polycaprolactone (PCL) coating on fluoride treated (MgF2) biodegradable ZK60 magnesium alloy (Mg) for biomedical application. MgF2 conversion layer was first produced by immersing Mg alloy substrate in hydrofluoric acid solution. The outer PCL coating was then prepared using dip coating technique. Morphology, elements profile, phase structure, roughness, mechanical properties, invitro corrosion, and biocompatibility of duplex MgF2/PCL coating were then characterized and compared to those of fluoride coated and uncoated Mg samples...
2018: PloS One
https://www.readbyqxmd.com/read/29597169/synthesis-and-evaluation-of-novel-coumarin-oxime-ethers-as-potential-anti-tubercular-agents-their-dna-cleavage-ability-and-bsa-interaction-study
#10
Dinesh S Reddy, Manasa Kongot, Sandeep P Netalkar, Mahantesh M Kurjogi, Rakesh Kumar, Fernando Avecilla, Amit Kumar
As a contribution to the development of novel coumarin-oxime ether conjugates with therapeutically interesting properties, a series of coumarin-oxime ether (1a-1j) was synthesised using SN 2 reaction of bromomethyl coumarins with butane-2,3-dione monoxime. Invitro anti-tuberculosis activityagainstMTBH37 Rv strain was established for the coumarin-oxime ether (1a-1j). Most of the compounds exhibited significant activity with minimum inhibitory concentration (MIC)in the range of 0.04-3.12 μg mL-1 . Compound (1h) was identified as a hit candidate exhibiting MIC of 0...
March 20, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29563529/non-heuristic-automatic-techniques-for-overcoming-low-signal-to-noise-ratio-bias-of-localization-microscopy-and-multiple-signal-classification-algorithm
#11
Krishna Agarwal, Radek Macháň, Dilip K Prasad
Localization microscopy and multiple signal classification algorithm use temporal stack of image frames of sparse emissions from fluorophores to provide super-resolution images. Localization microscopy localizes emissions in each image independently and later collates the localizations in all the frames, giving same weight to each frame irrespective of its signal-to-noise ratio. This results in a bias towards frames with low signal-to-noise ratio and causes cluttered background in the super-resolved image. User-defined heuristic computational filters are employed to remove a set of localizations in an attempt to overcome this bias...
March 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29462679/novel-ph-sensitive-dual-drug-loaded-gelatin-methacrylate-methacrylic-acid-hydrogel-for-the-controlled-release-of-antibiotics
#12
Thayyath Sreenivasan Anirudhan, Anila Manasa Mohan
The aim of the present study was to develop a novel pH sensitive gelatin methacrylate hydrogel for the controlled delivery of Gentamicin (GS) and Ampicillin (Amp). GS and Amp having synergistic activity is effective in killing multi drug resistant bacteria. The hydrogel was well characterized using FTIR, XRD and SEM techniques. The drug loading and encapsulation efficiency were found to be 85.0 and 77.0% for GS, 79.0 and 88.0% for Amp, respectively. The in vitro swelling, degradation and release profiles suggest the pH dependent behaviour of hydrogel...
April 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29447972/novel-asymmetric-chitosan-pvp-nanocellulose-wound-dressing-in-vitro-and-in-vivo-evaluation
#13
R Poonguzhali, S Khaleel Basha, V Sugantha Kumari
The present study was to develop a novel chitosan based symmetric and asymmetric bionanocomposite for potential wound dressing application. Chitosan (C)/Poly (vinyl pyrrolidone) (P)/nanocellulose (NC) membrane were fabricated by salt leaching method with the addition of 3% and 5% wt of nanocellulose. To obtain asymmetric material one side of the membrane was coated by stearic acid (S) which could form hydrophobic surface and another side acts as a hydrophilic surface. Nanocellulose of size 2-10nm was synthesized and characterized by TEM analysis...
June 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29435494/tick-borne-encephalitis-virus-vaccine-induced-human-antibodies-mediate-negligible-enhancement-of-zika-virus-infection-in-vitro-and-in-a-mouse-model
#14
James Duehr, Silviana Lee, Gursewak Singh, Gregory A Foster, David Krysztof, Susan L Stramer, Maria C Bermúdez González, Eva Menichetti, Robert Geretschläger, Christian Gabriel, Viviana Simon, Jean K Lim, Florian Krammer
Recent reports in the scientific literature have suggested that anti-dengue virus (DENV) and anti-West Nile virus (WNV) immunity exacerbates Zika virus (ZIKV) pathogenesis in vitro and in vivo in mouse models. Large populations of immune individuals exist for a related flavivirus (tick-borne encephalitis virus [TBEV]), due to large-scale vaccination campaigns and endemic circulation throughout most of northern Europe and the southern Russian Federation. As a result, the question of whether anti-TBEV immunity can affect Zika virus pathogenesis is a pertinent one...
January 2018: MSphere
https://www.readbyqxmd.com/read/29386152/neurite-outgrowth-properties-of-calotropis-procera-in-search-for-a-neuroprotectant
#15
Aisha Kamal, Zafar Saeed Saify, Khawar Saeed Jamali, Priya Tufail, Aiman Kanwal, Faisal Khan, Sonia Siddiqui
In view of the well-documented medicinal properties of Calotropis procera (CP), the present study was designed to evaluate the neuroprotective effect of the extract. We have prepared a methanolic extract of Calotropis procera and screen varying concentration of CP (20, 30, 40, 50 and 70μg/ml) for the stimulatory potency on neurite outgrowth. The stimulatory effect of CP on neurite outgrowth was assessed in primary hippocampal neurons. Neurite lengths were measured using optika provison analysis software. Neuritogenesis was further analyzed by immunostaining by using specific neuronal marker β III-tubulin...
January 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29364278/isolation-culture-and-differentiation-of-bone-marrow-stromal-cells-and-osteoclast-progenitors-from-mice
#16
David E Maridas, Elizabeth Rendina-Ruedy, Phuong T Le, Clifford J Rosen
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro...
January 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29295667/in-vivo-evidence-of-inflammasome-activation-during-spontaneous-labor-at-term
#17
Bogdan Panaitescu, Roberto Romero, Nardhy Gomez-Lopez, Yi Xu, Yaozhu Leng, Eli Maymon, Percy Pacora, Offer Erez, Lami Yeo, Sonia S Hassan, Chaur-Dong Hsu
OBJECTIVE: Upon inflammasome activation, the adaptor protein of the inflammasome ASC (apoptosis-associated speck-like protein containing a CARD) forms intracellular specks, which can be released into the extracellular space. The objectives of this study were to investigate whether (1) extracellular ASC is present in the amniotic fluid of women who delivered at term; (2) amniotic fluid ASC concentrations are greater in women who underwent spontaneous labor at term than in those who delivered at term in the absence of labor; and (3) amniotic epithelial and mesenchymal cells can form intracellular ASC specks in vitro...
January 17, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29228221/oncogenic-potential-of-hepatitis-b-virus-subgenotype-d1-surpasses-d3-significance-in-the-development-of-hepatocellular-carcinoma
#18
Somenath Datta, Debanjali Dasgupta, Alip Ghosh, Suchandrima Ghosh, Alak Manna, Simanti Datta, Mitali Chatterjee, Abhijit Chowdhury, Soma Banerjee
Despite widespread distribution of hepatitis B virus (HBV)-genotype D, the clinical implications of its ten subgenotypes (D1-D10) have not been well documented. Here, we have investigated the impact of two major circulating HBV/D subgenotypes, D1 and D3 in Eastern India towards pathogenesis of liver disease progression to hepatocellular carcinoma (HCC). HBV subgenotypes were determined using full-length genome sequences of HBV isolates from patients with chronic hepatitis B (CHB), liver cirrhosis (LC) and HCC...
February 9, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29184631/comparison-of-the-accuracy-of-conventional-and-digital-radiography-in-root-canal-working-length-determination-an-invitro-study
#19
Mohammad Mahdi Yaghooti Khorasani, Hamed Ebrahimnejad
Background. Digital radiography has widespread use in endodontics. Determining a correct working length is vital for a proper endodontic therapy. The aim of this study was to compare the accuracy of conventional and digital radiographic techniques for root canal working length determination. Methods. After determining the real working lengths of 50 permanent maxillary central incisors (gold standard), the conventional (E- and F-speed films) and digital (CCD, PSP) images were obtained using the parallel technique...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/29103204/invitro-evaluation-of-torin2-and-2-6-dihydroxyacetophenone-in-colorectal-cancer-therapy
#20
Ankita Awasthi, Pharvendra Kumar, Chittur V Srikanth, Shakti Sahi, Rekha Puria
Colorectal cancer (CRC) is one of the most prevalent cancers diagnosed worldwide. Despite recent advances, resistance to cytotoxic and targeted therapy remains one of the greatest challenges in long-term management of colorectal cancer therapy. Recently established role of mTOR signaling in proliferation of CRC has incited for evaluation of mTOR kinase specific inhibitors in CRC therapy. Second generation mTOR kinase inhibitors including Torin2 has demonstrated efficient anticancer properties against variety of cancers and are in various stages of drug development...
November 4, 2017: Pathology Oncology Research: POR
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