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https://www.readbyqxmd.com/read/27902419/influence-of-tick-and-mammalian-physiological-temperatures-on-borrelia-burgdorferi-biofilms
#1
Shafiq Shaikh, Venkata Arun Timmaraju, Jason P Torres, Kayla M Socarras, Priyanka A S Theophilus, Eva Sapi
The spirochaete bacterium Borrelia burgdorferisensu lato is the aetiologic agent of Lyme disease. Borrelia is transmitted to mammals through tick bite and is adapted to survive at tick and mammalian physiological temperatures. We have previously shown that B. burgdorferi can exist in different morphological forms, including the antibiotic-resistant biofilm form, in vitro and in vivo. B. burgdorferi forms aggregates in ticks as well as in humans, indicating potential of biofilm formation at both 23 and 37 °C...
November 2016: Microbiology
https://www.readbyqxmd.com/read/27882005/can-blood-and-semen-presepsin-levels-in-males-predict-pregnancy-in-couples-undergoing-intra-cytoplasmic-sperm-injection
#2
Ali Ovayolu, Cansev Yilmaz Arslanbuğa, Ismet Gun, Belgin Devranoglu, Arman Ozdemir, Sule Eren Cakar
OBJECTIVE: To determine whether semen and plasma presepsin values measured in men with normozoospermia and oligoasthenospermia undergoing invitro-fertilization would be helpful in predicting ongoing pregnancy and live birth. METHODS: Group-I was defined as patients who had pregnancy after treatment and Group-II comprised those with no pregnancy. Semen and blood presepsin values were subsequently compared between the groups. Parametric comparisons were performed using Student's t-test, and non-parametric comparisons were conducted using the Mann-Whitney U test...
September 2016: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/27827458/microrna-126-overexpression-rescues-diabetes-induced-impairment-in-efferocytosis-of-apoptotic-cardiomyocytes
#3
Sahana Suresh Babu, Rajarajan A Thandavarayan, Darukeshwara Joladarashi, Prince Jeyabal, Shashirekha Krishnamurthy, Arvind Bhimaraj, Keith A Youker, Prasanna Krishnamurthy
Efferocytosis, a process of clearance of apoptotic cells by phagocytes, is essential for successful resolution of inflammation and maintenance of tissue homeostasis. Diabetes compromises the function of macrophages leading to adverse inflammatory response during wound healing, myocardial injury, atherosclerosis and autoimmune disorders. However, the effect of diabetes on macrophage-mediated efferocytosis of apoptotic cardiomyocytes (ACM) and the molecular mechanisms involved are not understood so far. In the present study we found that invitro efferocytosis of ACM was impaired in macrophages from db/db (diabetic) mice...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27809851/cyclophilin-a-enhances-macrophage-differentiation-and-lipid-uptake-in-high-glucose-conditions-a-cellular-mechanism-for-accelerated-macro-vascular-disease-in-diabetes-mellitus
#4
Surya Ramachandran, Anandan Vinitha, Cheranellore Chandrasekharan Kartha
BACKGROUND: Vascular disease in diabetes is initiated by monocyte adhesion to vascular endothelium, transmigration and formation of foam cells. Increasing clinical evidence supports a role for the secretory protein, cyclophilin A in diabetic vascular disease. The means by which cyclophilin A contributes to vascular lesion development in diabetes is however largely unknown. METHODS: In this study we investigated using THP1 cells and human monocytes whether cyclophilin A under hyperglycemic conditions, functions in the inflammatory cascade as a chemoattractant and increases lipid uptake by formation of foam cells invitro...
November 3, 2016: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/27790583/comparative-assessment-of-antimicrobial-efficacy-of-different-antibiotic-coated-gutta-percha-cones-on-enterococcus-faecalis-an-invitro-study
#5
Vardhaman Mulchand Jain, Gundabaktha Nagappa Karibasappa, Arun Suresh Dodamani, Prashanth Kumar Vishwakarma, Gaurao Vasant Mali
INTRODUCTION: The major goal of endodontic treatment is to eliminate bacteria from the root canals and prevent re-infection. Enterococcus faecalis (E. faecalis) has been attributed to be the most common organism for the endodontic treatment failures. The choice of endodontic material that have high antimicrobial efficacy can help in decreasing/avoiding growth of micro-organisms and facilitate the success rate of treatment. AIM: The present study was designed with an aim to evaluate the antimicrobial efficacy of antibiotic coated gutta-percha cones on E...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790568/comparative-evaluation-of-the-marginal-sealing-ability-of-two-commercially-available-pit-and-fissure-sealants
#6
Lakshmi Priya Sridhar, Joyson Moses, Bollam Nammalwar Rangeeth, Subramanian Sivakumar
INTRODUCTION: The occlusal surfaces of the teeth usually have pits and fissures which provide a good environment for demineralization with minimal salivary access and make them caries prone. The success of pit and fissure sealing materials is highly dependent on the marginal sealing ability of the fissures. AIM: The present study was carried out to evaluate and compare the marginal sealing ability of two commercially available pit and fissure sealants. MATERIALS AND METHODS: A total of 50 human premolar teeth were used in this invitro study...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790459/estimation-of-antimicrobial-properties-of-aqueous-and-alcoholic-extracts-of-salvadora-persica-miswak-on-oral-microbial-pathogens-an-invitro-study
#7
Salman Siddeeqh, Amrita Parida, Maji Jose, Vidya Pai
INTRODUCTION: Twigs of Salvadora persica (Miswak) plant are being used as a means of oral hygiene since ages for brushing teeth. Though clinical research and trials have shown promising results on effectiveness of Miswak, but some reports are conflicting. AIM: To evaluate the antimicrobial activity of crude aqueous and alcoholic extracts of Salvadora persica (Miswak) against the common microbial pathogens causing dental caries and periodontitis. MATERIALS AND METHODS: A prospective study of one year duration was conducted in Yenopoya dental and medical college, Mangalore...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790457/in-vitro-comparison-of-generic-and-branded-preparations-of-amoxicillin-with-potassium-clavulanate
#8
Priyanka Pathak, Jayshree Dawane
INTRODUCTION: The Medical Council of India (MCI) has guidelines for physicians to prescribe drugs with generic names. But physicians and patients might have prejudices against generic drug substitution or concerns regarding quality and generics effectiveness. If the drugs are ineffective, they can result in adverse clinical outcomes such as treatment failure. According to WHO, β-lactams are the most common substandard drugs to be produced amongst antibiotics. AIM: To evaluate and compare invitro efficacy of generic and branded preparations of Amoxicillin with Potassium Clavulanate...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790432/invitro-activities-of-polymyxins-and-rifampicin-against-carbapenem-resistant-acinetobacter-baumannii-at-a-tertiary-care-hospital-from-south-india
#9
Dheepa Muthusamy, S Sudhishnaa, Appalaraju Boppe
INTRODUCTION: Acinetobacter baumannii (A.baumannii) is rapidly emerging as a potent organism causing a multitude of nosocomial infections. The organism also carries various resistance mechanisms to antibiotics, making treatment more difficult. Very few choices are left, as A.baumannii strains have begun to develop resistance against cephalosporins, aminoglycosides and even carbapenems. AIM: To examine the sensitivity pattern of three older antibiotics namely colistin, polymyxin B and rifampicin against carbapenem resistant A...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27738917/amelioration-of-oxidative-and-inflammatory-changes-by-swertia-chirayita-leaves-in-experimental-arthritis
#10
Harsha Lad, Deepak Bhatnagar
The present study was aimed to determine the therapeutic effects of Swertia chirayita leaves against oxidative and inflammatory injuries in Freund's complete adjuvant (FCA) induced arthritic rats. The extract was evaluated for its phytoconstituents and various invitro antioxidant properties followed by its in vivo effects. The hydroethanolic extract of S. chirayita leaves (SCE) was orally administered (200 mg/kg body weight, per day, p.o.) and the effect on the liver lipid peroxidation (LPO), antioxidant status, protein carbonyl formation along with the histopathology of liver were evaluated after induction of adjuvant arthritis...
October 14, 2016: Inflammopharmacology
https://www.readbyqxmd.com/read/27725960/drug-delivery-via-cell-membrane-fusion-using-lipopeptide-modified-liposomes
#11
Jian Yang, Azadeh Bahreman, Geert Daudey, Jeroen Bussmann, René C L Olsthoorn, Alexander Kros
Efficient delivery of drugs to living cells is still a major challenge. Currently, most methods rely on the endocytotic pathway resulting in low delivery efficiency due to limited endosomal escape and/or degradation in lysosomes. Here, we report a new method for direct drug delivery into the cytosol of live cells in vitro and invivo utilizing targeted membrane fusion between liposomes and live cells. A pair of complementary coiled-coil lipopeptides was embedded in the lipid bilayer of liposomes and cell membranes respectively, resulting in targeted membrane fusion with concomitant release of liposome encapsulated cargo including fluorescent dyes and the cytotoxic drug doxorubicin...
September 28, 2016: ACS Central Science
https://www.readbyqxmd.com/read/27721563/comparison-of-shear-bond-strength-of-reattached-incisor-fragment-using-er-cr-ysgg-laser-etching-and-conventional-acid-etching-an-in-vitro-study
#12
Ferah Rehman, Gyanendra Kumar, Mridula Goswami, Jatinder Kaur Dhillon
Aim: The aim of this invitro study is to evaluate the shear bond strength of reattached fractured incisor fragments using Er,Cr:YSGG laser and conventional acid etching without additional tooth preparation. Materials and methods: Forty extracted human teeth were divided in two groups of 20 each (Groups A and B). In Group A, fractured surface was treated by an Er, Cr: YSGG laser system (Waterlase MD, Biolase Technology Inc., San Clemente, CA, USA) operating at a wavelength of 2,780 nm and frequency of 20 Hz...
June 29, 2016: Laser Therapy
https://www.readbyqxmd.com/read/27686121/-design-synthesis-and-evaluation-of-pyrazolo-pyrazole-derivatives-onmethylisocitratelyase-of-pseudomonas-aeruginosa-insilico-and-invitro-study
#13
Madhusudana Pulaganti, Anuradha C M, Chitta Suresh Kumar
Pseudomonas aeruginosa (P.aeruginosa) is an opportunistic microorganism causing diseases both in animals and humans. In case of human pathology, the role of P.aeruginosa is one of the major concerns in intensive care septicemia. Presently the drug resistance strains of P.aeruginosa are arising mainly by developing multiple mechanisms due to its natural and acquired resistance to many of the antimicrobial agents commonly used in clinical practice. As a result, there is a direneed to invent new drugs so that they may restrict the outbreak of multidrug resistant strains...
August 15, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27640606/evaluation-of-an-ultrafast-molecular-rotor-auramine-o-as-a-fluorescent-amyloid-marker
#14
Niyati H Mudliar, Biswajit Sadhu, Aafrin M Pettiwala, Prabhat K Singh
Recently, Auramine O (AuO) has been projected as a fluorescent fibril sensor, and it has been claimed that AuO has an advantage over the most extensively utilized fibril marker, Thioflavin-T (ThT), owing to the presence of an additional large red-shifted emission band for AuO, which was observed exclusively for AuO in the presence of fibrillar media and not in protein or buffer media. As fibrils are very rich in β-sheet structure, a fibril sensor should be more specific toward the β-sheet structure so as to produce a large contrast between the fibril form and native protein form, for efficient detection and in vitro mechanistic studies of fibrillation...
October 13, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27630957/comparative-morphologic-evaluation-and-occluding-effectiveness-of-nd-yag-co2-and-diode-lasers-on-exposed-human-dentinal-tubules-an-invitro-sem-study
#15
Mini Saluja, Harpreet Singh Grover, Pankaj Choudhary
INTRODUCTION: Dentinal hypersensitivity is one of the most common problem, encountered in dental practice but has least predictable treatment outcome. The advent of lasers in dentistry has provided an additional therapeutic option for treating dentinal hypersensitivity. Although various lasers have been tried over a period of time to treat dentinal hypersensitivity, but still the doubt persist as to which laser leads to maximum dentinal tubular occlusion and is most suitable with minimal hazardous effects...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27630943/nanoleakage-evaluation-of-posterior-teeth-restored-with-low-shrinkable-resin-composite-an-invitro-study
#16
Labib Mohamed Labib, Sameh Mahmoud Nabih, Kusai Baroudi
INTRODUCTION: The effect of nanoleakage on the integrity of resin-dentin bond has been in interest for long-term adhesion. AIM: This study evaluated the nanoleakage in premolar teeth restored with low shrinkable resin composite. MATERIALS AND METHODS: A total of 40 human premolars were used for nanoleakage evaluation in this study. Each group was divided into four equal groups; Group A: using silorane with its adhesive system. Group B: using silorane with G-bond...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27613330/invitro-and-in-vivo-antitumor-activity-of-a-novel-carbonyl-ruthenium-compound-the-ct-rucl-co-dppb-bipy-pf-6-dppb-1-4-bis-diphenylphosphine-butane-and-bipy-2-2-bipyridine
#17
Andréa P Carnizello, Marília I F Barbosa, Monize Martins, Natália H Ferreira, Pollyanna F Oliveira, Geórgia M Magalhães, Alzir A Batista, Denise C Tavares
This study performed in vitro and in vivo biological assays of the ruthenium (II) compound ct-[RuCl(CO)(dppb)(bipy)]PF6 (where, dppb=1,4-bis(diphenylphosphine)butane and bipy=2,2'-bipyridine). The cytotoxic activity of this compound was evaluated against different tumor cell lines (HeLa, human cervical adenocarcinoma; MCF7, human breast adenocarcinoma; MO59J, human glioblastoma; HepG2, hepatocellular carcinoma and B16F10, murine melanoma) and healthy cell line (V79, Chinese hamster lung fibroblasts), by XTT (sodium 2,3'-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-3,4-tetrazolium-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) method...
August 25, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27538988/progesterone-augments-cell-susceptibility-to-hiv-1-and-hiv-1-hsv-2-co-infections
#18
Viswanath Ragupathy, Wang Xue, Ji Tan, Krishnakumar Devadas, Yamei Gao, Indira Hewlett
In human immunodeficiency virus type 1 (HIV-1)-infected women, oral or injectable progesterone containing contraceptive pills may enhance HIV-1 acquisition in vivo, and the mechanism by which this occurs is not fully understood. In developing countries, Herpes simplex virus type-2 (HSV-2) co-infection has been shown to be a risk for increase of HIV-1 acquisition and, if co-infected women use progesterone pills, infections may increase several fold. In this study, we used an in vitro cell culture system to study the effects of progesterone on HIV-1 replication and to explore the molecular mechanism of progesterone effects on infected cells...
October 2016: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/27509927/pleiotropic-roles-of-metalloproteinases-in-hematological-malignancies-an-update
#19
REVIEW
Ajay K Chaudhary, Shruti Chaudhary, Kanjaksha Ghosh, A Nadkarni
Controlled remodeling of the extracellular matrix (ECM) is essential for cell growth, invasion and metastasis. Matrix metalloproteinases (MMPs) are a family of secreted, zincdependent endopeptidases capable of degradation of ECM components. The expression and activity of MMPs in a variety of human cancers have been intensively studied. They play important roles at different steps of malignant tumor formation and have central significance in embryogenesis, tissue remodeling, inflammation, angiogenesis and metastasis...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27509925/tyrosine-kinase-inhibitors-in-ph-chronic-myeloid-leukemia-therapy-a-review
#20
REVIEW
Krupa Shah, Sonia Parikh, Rakesh Rawal
Chronic myeloid leukaemia (CML) is a clonal myeloproliferative hematopoietic stem cell disorder. Deregulated BCRABL fusion tyrosine kinase activity is the main cause of CML disease pathogenesis, making BCRABL an ideal target for inhibition. Current tyrosine kinase inhibitors (TKIs) designed to inhibit BCRABL oncoprotein activity, have completely transformed the prognosis of CML. Interruption of TKI treatment leads to minimal residual disease reside (MRD), thought to reside in TKIinsensitive leukaemia stem cells which remain a potential reservoir for disease relapse...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
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