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https://www.readbyqxmd.com/read/28212980/biofilm-formation-and-release-of-fluoride-from-dental-restorative-materials-in-relation-to-their-surface-properties
#1
Sebastian Hahnel, Andrei C Ionescu, Gloria Cazzaniga, Marco Ottobelli, Eugenio Brambilla
OBJECTIVES: To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation. METHODS: Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface analyses. Subsequent to simulation of salivary pellicle formation, Streptococcus mutans biofilm formation was initiated using a drip flow reactor for 48h and 96h...
February 14, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28194363/in-vitro-study-of-streptococcus-mutans-adhesion-on-composite-resin-coated-with-three-surface-sealants
#2
Da Hye Kim, Tae-Yub Kwon
OBJECTIVES: Although the coating of surface sealants to dental composite resin may potentially reduce bacterial adhesion, there seems to be little information regarding this issue. This preliminary in vitro study investigated the adhesion of Streptococcus mutans (S. mutans) on the dental composite resins coated with three commercial surface sealants. MATERIALS AND METHODS: Composite resin (Filtek Z250) discs (8 mm in diameter, 1 mm in thickness) were fabricated in a mold covered with a Mylar strip (control)...
February 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28193570/live-and-heat-killed-lactobacillus-spp-interfere-with-streptococcus-mutans-and-streptococcus-oralis-during-biofilm-development-on-titanium-surface
#3
E Ciandrini, R Campana, W Baffone
OBJECTIVES: This research investigates the ability of live and heat-killed (HK) Lactic Acid Bacteria (LAB) to interfere with Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 during biofilm formation. DESIGN: Eight Lactobacillus spp. and two oral colonizers, pathogenic Streptococcus mutans and resident Streptococcus oralis, were characterized for their aggregation abilities, cell surface properties and biofilm formation ability on titanium surface...
February 4, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28187901/enhanced-immune-response-to-a-dual-promoter-anti-caries-dna-vaccine-orally-delivered-by-attenuated-salmonella-typhimurium
#4
Hao Jiang, Yijun Hu, Mei Yang, Hao Liu, Guangshui Jiang
The strength of immune responses induced by DNA vaccine is closely associated with the expression level of cloned antigens available to the antigen presenting cells (APCs). To acquire a larger and more persistent amount of antigen, a dual-promoter, which could double the target antigen output through its expression both in prokaryotic and eukaryotic cells, was employed in the constructed anti-caries DNA vaccine with attenuated Salmonella as mucosal delivery vector in this study. Here, both CMV and nirB promoters were included in the plasmid that harbors the genes encoding the functional epitopes of two virulence factors of S...
January 25, 2017: Immunobiology
https://www.readbyqxmd.com/read/28183617/physical-chemical-and-antimicrobial-evaluation-of-a-composite-material-containing-quaternary-ammonium-salt-for-braces-cementation
#5
Mari Miura Sugii, Fábio Augusto de Souza Ferreira, Karina Cogo Müller, Debora Alves Nunes Leite Lima, Francisco Carlos Groppo, Hidetake Imasato, Ubirajara Pereira Rodrigues-Filho, Flávio Henrique Baggio Aguiar
The antibiofilm effect of iodide quaternary ammonium methacryloxy silicate (IQAMS) in Transbond XT Light Cure Adhesive resin used for braces cementation was evaluated. Fourier Transform Infrared (FTIR) spectroscopy confirmed IQAMS formation and Scanning Electron Microscopy coupled to Energy-Dispersive X-ray Spectroscopy (SEM-EDS) revealed that as coating, the quaternary ammonium groups from IQAMS were homogeneously dispersed throughout the surface. When incorporated, the composite material presented homogeneous dispersion throughout the resin...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28182053/correlation-between-dental-caries-experience-and-mutans-streptococci-counts-by-microbial-and-molecular-polymerase-chain-reaction-assay-using-saliva-as-microbial-risk-indicator
#6
S G Damle, Ashish Loomba, Abhishek Dhindsa, Ashu Loomba, Vikas Beniwal
BACKGROUND: The aim of this study was to assess the relationships of quantitative salivary levels of mutans streptococci (MS) in children, aged 3-6 years and 12-15 years, exhibiting variable patterns of caries activity, and to compare the association of MS in saliva using microbial and molecular (polymerase chain reaction [PCR]) assay. MATERIALS AND METHODS: In this cross-sectional observational study, eighty children were included, forty children each in Group I (3-6 years) and Group II (12-15 years)...
November 2016: Dental Research Journal
https://www.readbyqxmd.com/read/28181353/antimicrobial-effect-frictional-resistance-and-surface-roughness-of-stainless-steel-orthodontic-brackets-coated-with-nanofilms-of-silver-and-titanium-oxide-a-preliminary-study
#7
Tania Ghasemi, Valiollah Arash, Vahid Rakhshan, Mahmoud Rabiee, Ramezan Rajab Nia, Fatemeh Majd Zadeh, Amir Hossein Pour Zare, Mana Shamsedin, Samira Kavoli
Nano-silver and nano-titanium oxide films can be coated over brackets in order to reduce bacterial aggregation and friction. However, their antimicrobial efficacy, surface roughness, and frictional resistance are not assessed before. Fifty-five stainless-steel brackets were divided into 5 groups of 11 brackets each: uncoated brackets, brackets coated with 60 µm silver, 100 µm silver, 60 µm titanium, and 100 µm titanium. Coating was performed using physical vapor deposition method. For friction test, three brackets from each group were randomly selected and tested...
February 9, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28177575/surface-coating-of-gypsum-based-molds-for-maxillofacial-prosthetic-silicone-elastomeric-material-evaluating-different-microbial-adhesion
#8
Salah Khalaf, Zaihan Ariffin, Adam Husein, Fazal Reza
PURPOSE: To compare the adhesion of three microorganisms on modified and unmodified silicone elastomer surfaces with different surface roughnesses and porosities. MATERIALS AND METHODS: Candida albicans, Streptococcus mutans, and Staphylococcus aureus were incubated with modified and unmodified silicone groups (N = 35) for 30 days at 37°C. The counts of viable microorganisms in the accumulating biofilm layer were determined and converted to cfu/cm(2) unit surface area...
February 8, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28175292/efflux-inhibitor-suppresses-streptococcus-mutans-virulence-properties
#9
Huihui Zeng, Jia Liu, Junqi Ling
No abstract text is available yet for this article.
February 8, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28169312/antibacterial-activity-and-bonding-ability-of-an-orthodontic-adhesive-containing-the-antibacterial-monomer-2-methacryloxylethyl-hexadecyl-methyl-ammonium-bromide
#10
Fan Yu, Yan Dong, Hao-Han Yu, Ping-Ting Lin, Ling Zhang, Xiang Sun, Yan Liu, Yu-Ning Xia, Li Huang, Ji-Hua Chen
Irreversible white spot lesion (WSL) occurs in up to 50% of patients during orthodontic treatment. Therefore, orthodontic adhesives need to be able to inhibit or reduce bacterial growth in order to prevent or minimize WSL. This study evaluated the antibacterial effect and shear bond strength (SBS) of a resin-based orthodontic adhesive containing the antibacterial monomer 2-methacryloxylethyl hexadecyl methyl ammonium bromide (MAE-HB). MAE-HB was added at three concentrations (1, 3, and 5 wt%) to a commercial orthodontic adhesive Transbond XT, while the blank control comprised unmodified Transbond XT...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28169264/in-vitro-study-of-the-effect-of-an-essential-oil-and-a-delmopinol-mouth-rinse-on-dental-plaque-bacteria
#11
Henrique Soares LuIs, Luis Soares Luis, Mário Bernardo
CONTEXT: Mouthrinses are used, by many of our patients, as a complement to daily dental hygiene routine. The use of a toothbrush and an interproximal cleaning method may not be enough to control dental plaque. Essential oils and delmopinol mouth rinses are effective for the prevention of dental caries and gingivitis. To study the effect of an essential oil and a delmopinol mouth rinse on dental plaque bacteria, an in vitro study was developed. AIMS: The objective of this study was to determine the antibacterial activity of an essential oil and a delmopinol mouth rinse on Streptococcus mutans, Lactobacilli, and aerobic and anaerobic dental plaque nonspecific bacteria...
November 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/28167518/pleiotropic-regulation-of-virulence-genes-in-streptococcus-mutans-by-a-conserved-small-protein-sprv
#12
Manoharan Shankar, Mohammad S Hossain, Indranil Biswas
: Streptococcus mutans, an oral pathogen associated with dental caries, colonizes tooth surfaces as polymicrobial biofilms, known as dental plaque. S. mutans expresses several virulence factors that allow the organism to tolerate environmental fluctuations and to compete with other microorganisms. We recently identified that a small hypothetical protein (90 amino acids) was essential for normal growth of the bacterium. Inactivation of the gene SMU.2137 encoding this protein caused a significant growth defect and loss of various virulence-associated functions...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28163849/evaluation-of-the-antibacterial-effects-of-aqueous-and-ethanolic-leaf-extracts-of-aloysia-citriodora-lemon-verbena-on-streptococcus-mutans-and-streptococcus-sobrinus
#13
Faranak Shafiee, Ali Akbar Moghadamnia, Zahra Shahandeh, Farhnaz Sadighian, Effat Khodadadi
INTRODUCTION: The Aloysia citriodora plant from the family of Verbenaceae has many uses in traditional medicine. The aim of the current study was to determine the effects of the aqueous and ethanolic extracts of A. citriodora on Streptococcus mutans and Streptococcus sobrinus, which cause tooth decay. METHODS: This 2016 study was performed on standardized strains of S. mutans PTCC1683 and S. sobrinus PTCC1601 and clinical isolates. Twenty clinical samples were obtained from the dental caries of children admitted to the pediatric ward at the Faculty of Dentistry of Babol University of Medical Sciences (Babol, Iran)...
December 2016: Electronic Physician
https://www.readbyqxmd.com/read/28149461/microbiological-characterization-and-effect-of-resin-composites-in-cervical-lesions
#14
Bonfanti Carlo, Nicola Barabanti, Giorgio Piccinelli, Vicente Faus-Matoses, Antonio Cerutti
BACKGROUND: Non carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect, in vivo and in vitro, against S. mutans. MATERIAL AND METHODS: Eight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28147347/ph-response-and-tooth-surface-solubility-at-the-tooth-bacteria-interface
#15
Gen Mayanagi, Koei Igarashi, Jumpei Washio, Nobuhiro Takahashi
Evaluating the physiochemical processes at the tooth surface/bacteria interface is important for elucidating the etiology of dental caries. This study aimed to compare the mineral solubility and protein degradation of coronal enamel (CE) and root dentin (RD), and investigate the involvement of dissolved components in bacteria-induced pH changes using a model of tooth/bacteria interface. An experimental apparatus forming a well was made of polymethyl methacrylate, and a bovine tooth (CE or RD) specimen was fixed at the bottom of the well...
February 2, 2017: Caries Research
https://www.readbyqxmd.com/read/28144851/application-of-diverse-natural-polymers-in-the-design-of-oral-gels-for-the-treatment-of-periodontal-diseases
#16
Auleep Ganguly, Cheng Kang Ian, Ravi Sheshala, Priyadarshi Soumyaranjan Sahu, Haider Al-Waeli, Venkata Srikanth Meka
The objective of this study was to prepare periodontal gels using natural polymers such as badam gum, karaya gum and chitosan. These gels were tested for their physical and biochemical properties and assessed for their antibacterial activity against Aggregatibacter actinomycetemcomitans and Streptococcus mutans, two pathogens associated with periodontal disease. Badam gum, karaya gum and chitosan were used to prepare gels of varying concentrations. Moxifloxacin hydrochloride, a known antimicrobial drug was choosen in the present study and it was added to the above gels...
March 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28142218/differential-effects-of-the-combination-of-tyrosol-with-chlorhexidine-gluconate-on-oral-biofilms
#17
Luana Ribeiro do Vale, Alberto Carlos Botazzo Delbem, Laís Salomão Arias, Renan Aparecido Fernandes, Ana Paula Miranda Vieira, Débora Barros Barbosa, Douglas Roberto Monteiro
OBJECTIVE: This study assessed the effect of tyrosol and chlorhexidine gluconate in combination against Candida albicans, Candida glabrata and Streptococcus mutans in the planktonic state or forming biofilms in vitro. MATERIALS AND METHODS: Checkerboard assays were performed for determination of minimum inhibitory concentration. Biofilms were cultivated during 24 h on specimens of acrylic resin and hydroxyapatite, and treated with the drugs alone or in combination twice a day for 1 min, during 3 days...
January 31, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28141493/functional-amyloids-in-streptococcus-mutans-their-use-as-targets-of-biofilm-inhibition-and-initial-characterization-of-smu_63c
#18
Richard N Besingi, Iwona B Wenderska, Dilani B Senadheera, Dennis G Cvitkovitch, Joanna R Long, Zezhang T Wen, L Jeannine Brady
Amyloids have been identified as functional components of the extracellular matrix of bacterial biofilms. Streptococcus mutans is an established etiologic agent of dental caries and biofilm dweller. In addition to the previously identified amyloidogenic adhesin P1 (aka AgI/II, PAc), we show that the naturally occurring Antigen A derivative of S. mutans wall-associated protein A (WapA), and the secreted protein SMU_63c, can also form amyloid fibrils. P1, WapA and SMU_63c were found to significantly influence biofilm development and architecture, and all three proteins were shown by immunogold electron microscopy to reside within fibrillar extracellular matrix of the biofilms...
January 28, 2017: Microbiology
https://www.readbyqxmd.com/read/28139481/effect-of-green-tea-mouth-rinse-on-streptococcus-mutans-in-plaque-and-saliva-in-children-an-in-vivo-study
#19
Anil Kumar Goyal, Manohar Bhat, Meenakshi Sharma, Mamta Garg, Abhishek Khairwa, Rakesh Garg
AIMS: This study was conducted to evaluate and compare the antimicrobial efficacy of green tea catechin as a mouth wash on colony count of Streptococcus mutans in children. MATERIALS AND METHODS: A sample size of thirty children was selected out of screened 290 children by simple random sampling between the age group of 7 and 12 years. The study was conducted over a period of 2 weeks. After 24 h of oral prophylaxis, the baseline samples were collected and each group was subjected to mouth rinsing with green tea mouth wash for 2 weeks and further plaque and saliva samples were collected at 1- and 2-week intervals from baseline...
January 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28139480/evaluation-of-role-of-fixed-orthodontics-in-changing-oral-ecological-flora-of-opportunistic-microbes-in-children-and-adolescent
#20
Chandresh Shukla, Rajkumar Maurya, Vinod Singh, Manisha Tijare
OBJECTIVE: To determine the prevalence and counts of Streptococcus mutans and Candida species in orthodontic fixed appliance therapy patients and comparison of the efficiency of manual and electronic toothbrushes on minimizing plaque by reducing S. mutans and Candida species in above-mentioned patients. MATERIALS AND METHODS: The study was carried out on total number of sixty patients scheduled for orthodontic treatment (age group: 13-18). Plaque samples were collected to determine oral carriage of S...
January 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
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