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Noha Samir Kabil, Amira Saad Badran, Mariem Osama Wassel
BACKGROUND: Antibacterial restorations can increase the success rate of minimum invasive dentistry especially in young permanent molars with deep carious lesions as an attempt to preserve maximum dental structure and avoid pulp exposure. Further research is warranted to evaluate different antibacterial agents. OBJECTIVES: This study aimed to evaluate the efficacy of adding chlorhexidine gluconate (CHX) or aqueous miswak (Salvadora persica) extract on the clinical performance and in vivo antibacterial activity of conventional anhydrous glass ionomer cement (GIC)...
October 21, 2016: International Journal of Paediatric Dentistry
Ivana Nedeljkovic, Kumiko Yoshihara, Jan De Munck, Wim Teughels, Bart Van Meerbeek, Kirsten L Van Landuyt
BACKGROUND: In spite of contradicting results, the high susceptibility of composites for secondary caries is still often associated with the bacterial growth-stimulating effect of released methacrylate monomers. However, most studies that showed this effect were performed with techniques having inherent limitations (spectrophotometry). OBJECTIVES: Therefore, our objective was to determine the effect of four methacrylate monomers (2-Hydroxyethyl methacrylate (HEMA), triethylene glycol dimethacrylate (TEGDMA), ethylene glycol dimethacrylate (EGDMA), diethylene glycol dimethacrylate (DEGDMA)) on the growth of two caries-associated bacteria, Streptococcus mutans and sobrinus, and one non-cariogenic species, Streptococcus sanguinis, using TaqMan quantitative polymerase chain reaction (qPCR) to quantify bacterial DNA...
October 20, 2016: Clinical Oral Investigations
Karolina Niska, Narcyz Knap, Anna Kędzia, Maciej Jaskiewicz, Wojciech Kamysz, Iwona Inkielewicz-Stepniak
Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide antimicrobial activity spectrum. However, controversial information on AgNPs toxicity limited their use in oral infections. Therefore, the aim of the present study was to evaluate the antibacterial activities against a panel of oral pathogenic bacteria and bacterial biofilms together with potential cytotoxic effects on human gingival fibroblasts of 10 nm AgNPs: non-functionalized - uncapped (AgNPs-UC) as well as surface-functionalized with capping agent: lipoic acid (AgNPs-LA), polyethylene glycol (AgNPs-PEG) or tannic acid (AgNPs-TA) using silver nitrate (AgNO3) as control...
2016: International Journal of Medical Sciences
A Tofiño-Rivera, M Ortega-Cuadros, D Galvis-Pareja, H Jiménez-Rios, L J Merini, M C Martínez-Pabón
BACKGROUND: Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. AIM: The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils METHODOLOGY: Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined...
October 17, 2016: Journal of Ethnopharmacology
Wei Qiu, Biao Ren, Huanqin Dai, Lixin Zhang, Qiong Zhang, Xuedong Zhou, Yuqing Li
OBJECTIVE: The aim of this study was to determine the inhibitory effect of eight antifungal drugs on S. mutans growth, biofilm formation and virulence factors. METHODS: The actions of antifungal drugs on S. mutans were determined by recovery plates and survival kinetic curves. Biofilms were observed by scanning electron microscopy and the viable cells were recovered on BHI plates, meanwhile biofilms were stained by BacLight live/dead kit to investigate the biofilm viability...
October 13, 2016: Archives of Oral Biology
Xiaobei Wang, Huaixu Cheng, Mingsheng Lu, Yaowei Fang, Yuliang Jiao, Weijuan Li, Gengmao Zhao, Shujun Wang
Dental plaque is a biofilm of water-soluble and water-insoluble polysaccharides, produced primarily by Streptococcus mutans. Dextranase can inhibit biofilm formation. Here, a dextranase gene from the marine microorganism Arthrobacter oxydans KQ11-1 is described, and cloned and expressed using E. coli DH5α competent cells. The recombinant enzyme was then purified and its properties were characterized. The optimal temperature and pH were determined to be 60°C and 6.5, respectively. High-performance liquid chromatography data show that the final hydrolysis products were glucose, maltose, maltotriose, and maltotetraose...
November 2016: Biofouling
Zaleha Shafiei, Zubaidah Haji Abdul Rahim, Koshy Philip, Nalina Thurairajah
BACKGROUND: Plant extracts mixture (PEM) and its individual constituent plant extracts(Psidium sp., Mangifera sp., Mentha sp.) are known to have an anti-adhering effect towards oral bacteria in the single-species biofilm. To date, the adhering ability of the early and late plaque colonisers (Streptococcus sanguinis and Streptococcus mutans) to PEM-treated experimental pellicle have not been investigated in dual-species biofilms. METHODS: Fresh leaves of these plants were used in the preparation of the respective aqueous extract decoctions...
2016: PeerJ
M ElSalhy, E Söderling, E Honkala, M Fontana, S Flannagan, A Kokaras, B J Paster, A Varghese, S Honkala
AIM: To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition. MATERIALS AND METHODS: Twenty eight healthy, 11-12-year-old schoolchildren with high MS counts (>10⊃5 CFU/mL) were included in this study. The children were screened with the Dentocult SM Strip Mutans test (Orion Diagnostica, Espoo, Finland) and examined using the International Caries Detection and Assessment System (ICDAS)...
September 2016: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
Z Zingler, M Pritsch, C J Lux, S Kneist
AIM: To evaluate the correlation between clinical and salivary microbial parameters during treatment with removable orthodontic appliances with or without use of fluoride mouth rinse. MATERIALS AND METHODS: A group of 48 patients completed this randomized, controlled, parallel-group, clinical pilot study. 24 patients of the test group (TG) rinsed after tooth brushing with a fluoride mouth rinse (100 ppm AmF/150 ppm NaF) while 21 patients of the control group (CG) did not...
September 2016: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
Dharmaprakash Viszwapriya, Ganapathy Ashwinkumar Subramenium, Solai Radhika, Shunmugiah Karutha Pandian
Streptococcus mutans, a multivirulent pathogen is considered the primary etiological agent in dental caries. Development of antibiotic resistance in the pathogen has created a need for novel antagonistic agents which can control the virulence of the organism and reduce resistance development. The present study demonstrates the in vitro anti-virulence potential of betulin (lup-20(29)-ene-3β,28-diol), an abundantly available plant triterpenoid against S. mutans UA159. Betulin exhibited significant dose dependent antibiofilm activity without affecting bacterial viability...
October 18, 2016: Antonie Van Leeuwenhoek
S S Garcia, Q Du, H Wu
The oral cavity is a dynamic environment characterized by hundreds of bacterial species, saliva, and an influx of nutrients and metal ions such as copper. Although there is a physiologic level of copper in the saliva, the oral cavity is often challenged with an influx of copper ions. At high concentrations copper is toxic and must therefore be strictly regulated by pathogens for them to persist and cause disease. The cariogenic pathogen Streptococcus mutans manages excess copper using the copYAZ operon that encodes a negative DNA-binding repressor (CopY), the P1-ATPase copper exporter (CopA), and the copper chaperone (CopZ)...
December 21, 2015: Molecular Oral Microbiology
Miao Ai, Zhiyun Du, Siqi Zhu, Hongjuan Geng, Xu Zhang, Qing Cai, Xiaoping Yang
OBJECTIVE: The object is to find a functional one-dimensional nanofibrous filler for composite resin, which is able to provide both efficient reinforcement and high antibacterial activity. METHODS: Hydroxyapatite (HA) nanowires were synthesized via hydrothermal technique using calcium oleate as the precursor. Polydopamine (PDA)-coated HA (HA-PDA) nanowires were prepared by soaking HA nanowires in dopamine (DA) aqueous solution. Silver nanoparticles (AgNPs)-laden HA (HA-PDA-Ag) nanowires were prepared via reduction reaction by adding silver nitrate and glucose into HA-PDA suspensions in DI water...
October 10, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
Shoji Takenaka, Masataka Oda, Hisanori Domon, Tatsuya Ohsumi, Yuki Suzuki, Hayato Ohshima, Hirofumi Yamamoto, Yutaka Terao, Yuichiro Noiri
An ideal antibiofilm strategy is to control both in the quality and quantity of biofilm while maintaining the benefits derived from resident microflora. Vizantin, a recently developed immunostimulating compound, has also been found to have antibiofilm property. This study evaluated the influence on biofilm formation of Streptococcus mutans in the presence of sulfated vizantin and biofilm development following bacterial adhesion on a hydroxyapatite disc coated with sulfated vizantin. Supplementation with sulfated vizantin up to 50 μM did not affect either bacterial growth or biofilm formation, whereas 50 μM sulfated vizantin caused the biofilm to readily detach from the surface...
October 11, 2016: Biochemical and Biophysical Research Communications
Jin Xiao, Yonghwi Moon, Lihua Li, Elena Rustchenko, Hironao Wakabayashi, Xiaoyi Zhao, Changyong Feng, Steven R Gill, Sean McLaren, Hans Malmstrom, Yanfang Ren, Robert Quivey, Hyun Koo, Dorota T Kopycka-Kedzierawski
INTRODUCTION: Candida albicans has been detected together with Streptococcus mutans in high numbers in plaque-biofilm from children with early childhood caries (ECC). The goal of this study was to examine the C. albicans carriage in children with severe early childhood caries (S-ECC) and the maternal relatedness. METHODS: Subjects in this pilot cross-sectional study were recruited based on a convenient sample. DMFT(S)/dmft(s) caries and plaque scores were assessed during a comprehensive oral exam...
2016: PloS One
Yuan Wei, Wei Qiu, Xue-Dong Zhou, Xin Zheng, Ke-Ke Zhang, Shi-Da Wang, Yu-Qing Li, Lei Cheng, Ji-Yao Li, Xin Xu, Ming-Yun Li
D-alanine (D-Ala) is an essential amino acid that has a key role in bacterial cell wall synthesis. Alanine racemase (Alr) is a unique enzyme that interconverts L-alanine and D-alanine in most bacteria, making this enzyme a potential target for antimicrobial drug development. Streptococcus mutans is a major causative factor of dental caries. The factors involved in the survival, virulence and interspecies interactions of S. mutans could be exploited as potential targets for caries control. The current study aimed to investigate the physiological role of Alr in S...
October 14, 2016: International Journal of Oral Science
Hang Yang, Yongli Bi, Xiaoran Shang, Mengyue Wang, Sara B Linden, Yunpeng Li, Yuhong Li, Daniel C Nelson, Hongping Wei
Streptococcus mutans often survives as a biofilm on the tooth surface and contributes to the development of dental caries. We investigated the efficacy of ClyR, an engineered chimeolysin, against S. mutans biofilms in physiological and cariogenic conditions. Susceptibility tests showed that ClyR was active against all clinical S. mutans isolates tested as well as S. mutans biofilms that displayed resistance to penicillin. S. mutans biofilms formed on hydroxyapatite discs in physiological sugar conditions and cariogenic conditions were reduced ∼2 logs and 3 logs after treatment with 100 μg/ml ClyR, respectively...
October 10, 2016: Antimicrobial Agents and Chemotherapy
Tahsin Raquib Abonti, Masato Kaku, Shunichi Kojima, Hiromi Sumi, Shotoku Kojima, Taeko Yamamoto, Yuka Yashima, Hidekazu Miyahara, Akitoshi Okino, Toshitsugu Kawata, Kazuo Tanne, Kotaro Tanimoto
The purpose of this study is to evaluate the sterilization effects of a newly developed low temperature multi gas plasma jet on oral pathogenic microorganisms (Streptococcus mutans [S. mutans], Lactobacillus fermentum [L. fermentum], Aggregatibacter actinomycetemcomitans [A. actinomycetemcomitans]). Plasma gas which generated from O2, N2, Ar and 50% (O2+N2) was irradiated to the microbes. Effect of O2 plasma irradiation on S. mutans under scanning electron microscopy (SEM) was also observed. O2 plasma was directly applied to dental plaque on human extracted tooth...
2016: Dental Materials Journal
Byalakere Rudraiah Chandra Shekar, Ramesh Nagarajappa, Rupal Singh, S Suma, Rupesh Thakur
BACKGROUND: The rise in disease incidence, increased resistance of pathogenic bacteria to currently used antibiotics and chemotherapeutics, opportunistic infections in immunocompromised individuals, and financial considerations in developing countries necessitates alternate preventive and treatment strategies for oral diseases. OBJECTIVE: The objective of the study is to assess the antimicrobial efficacy of triple and quadruple combinations of Acacia nilotica (AN), Murraya koenigii (Linn...
July 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
Bruno J Conti, Karina B Santiago, Eliza O Cardoso, Paula P Freire, Robson F Carvalho, Marjorie A Golim, José M Sforcin
OBJECTIVES: Dendritic cells (DCs) are antigen-presenting cells, essential for recognition and presentation of pathogens to T cells. Propolis, a resinous material produced by bees from various plants, exhibits numerous biological properties, highlighting its immunomodulatory action. Here, we assayed the effects of propolis on the maturation and function of human DCs. METHODS: DCs were generated from human monocytes and incubated with propolis and LPS. NF-κB and cytokines production were determined by ELISA...
October 7, 2016: Journal of Pharmacy and Pharmacology
Tomoka Ueno, Yasushi Shimada, Khairul Matin, Yuan Zhou, Ikumi Wada, Alireza Sadr, Yasunori Sumi, Junji Tagami
The aim of this study was to evaluate the signal intensity and signal attenuation of swept source optical coherence tomography (SS-OCT) for dental caries in relation to the variation of mineral density. SS-OCT observation was performed on the enamel and dentin artificial demineralization and on natural caries. The artificial caries model on enamel and dentin surfaces was created using Streptococcus mutans biofilms incubated in an oral biofilm reactor. The lesions were centrally cross sectioned and SS-OCT scans were obtained in two directions to construct a three-dimensional data set, from the lesion surface (sagittal scan) and parallel to the lesion surface (horizontal scan)...
July 2016: Journal of Medical Imaging
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