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https://www.readbyqxmd.com/read/29335683/sex-differences-in-salivary-parameters-of-caries-susceptibility-in-healthy-individuals
#1
Leonardo Victor Galvão-Moreira, Cláudia Monteiro de Andrade, Jéssica Francisca Fernandes de Oliveira, Maria Rosa Quaresma Bomfim, Patricia de Maria Silva Figueiredo, Luciana Salles Branco-de-Almeida
PURPOSE: To investigate salivary parameters of caries susceptibility in men and women in order to identify potential variations due to sexual dimorphism. MATERIALS AND METHODS: A total of 46 female and 24 male patients, aged 18-40 years, participated in this study. Unstimulated saliva was collected for the evaluation of flow rate, pH, secretory IgA, Snyder test scores, and Streptococcus mutans counts (confirmed by PCR assay). Statistical analysis included the Mann-Whitney, Kruskal-Wallis and Bonferroni tests, one-way ANOVA, and the Spearman correlation at a 5% significance level, followed by a general linear model and multiple linear regressions...
January 15, 2018: Oral Health & Preventive Dentistry
https://www.readbyqxmd.com/read/29333770/-construction-of-srta-deletion-mutant-of-streptococcus-mutans-by-an-in-frame-deletion-system
#2
Chen Xuan, Liu Haixia, Peng Xian, Zou Ling
OBJECTIVE: To construct srtA-gene deletion mutant of Streptococcus mutans (S. mutans) UA159 with IFDC2 cassette through overlapping polymerase chain reaction (PCR) and allelic homologous recombination. METHODS: First, the upstream and downstream fragments surrounding the srtA and IFDC2 cassette were PCR amplified and ligated through overlapping PCR. The resulting amplicon was transformed into UA159, and positive transformants were selected on BHI plates containing erythromycin...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29331510/effects-of-sub-minimum-inhibitory-concentrations-of-lemon-essential-oil-on-the-acid-tolerance-and-biofilm-formation-of-streptococcus-mutans
#3
Yanwei Sun, Sijia Chen, Chen Zhang, Yali Liu, Li Ma, Xiangyu Zhang
OBJECTIVES: Lemon essential oil (LEO) is a kind of secondary metabolite from lemon peels and has been found to inhibit cariogenic bacteria for decades. However, its effects on main cariogenic virulence factors are rarely reported. The present study aimed to investigate the effects of sub-minimum inhibitory concentrations (sub-MICs) of LEO on the acid tolerance and biofilm formation of Streptococcus mutans (S. mutans) and preliminarily reveal the possible underlying mechanisms. DESIGNS: Effects of LEO on the acid tolerance and biofilm formation of S...
December 29, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29329652/three-new-anthraquinone-derivatives-isolated-from-symplocos-racemosa-and-their-antibiofilm-activity
#4
Umar Farooq, Sara Khan, Sadia Naz, Ajmal Khan, Afsar Khan, Ayaz Ahmed, Abdur Rauf, Syed Majid Bukhari, Shujaat Ali Khan, Arfa Kamil, Nadia Riaz, Abdur Rahman Khan
Three new alkyl substituted anthraquinone derivatives, trivially named as symploquinones A-C (Compounds 1-3) were isolated from Symplocos racemosa. The structures of these compounds were determined on the basis of extensive spectroscopic analyses (UV, IR, Mass, 1H- and 13C-NMR, and two-dimensional (2D) NMR techniques). The resulting data were also compared with the reported literature. These compounds were then subjected to antibacterial or antibiofilm testing. Compounds 1 and 3 exhibited good antibacterial activity in the concentration range of 160-83 μg·mL-1 against Streptococcus mutans, methicillin resistant Staphylococcus aureus and Proteus mirabilis...
December 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29328950/ultrastructural-damage-in-streptococcus-mutans-incubated-with-saliva-and-histatin-5
#5
A M Fernández-Presas, Y Márquez Torres, R García González, A Reyes Torres, I Becker Fauser, H Rodríguez Barrera, B Ruíz García, R Toloza Medina, J Delgado Domínguez, J L Molinarí Soriano
OBJECTIVE: To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS: S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. Ultrastructural damage was evaluated by transmission electron microscopy. Ultrastructural localization of histatin 5 was examined using immunogold labeling...
January 8, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29327482/streptococcus-oralis-maintains-homeostasis-in-supragingival-biofilms-by-antagonizing-cariogenic-pathogen-streptococcus-mutans
#6
Thomas Thurnheer, Georgios N Belibasakis
Bacteria residing in oral biofilms live in a state of dynamic equilibrium with one another. The intricate synergistic or antagonistic interactions between them are crucial for determining this balance. Using the 6-species Zürich "supragingival" biofilm model, this study aimed to investigate interactions regarding growth and localization of the constituent species. As control, an inoculum containing all six strains was used, whereas in each of the further five inocula one of the bacterial species was absent, and in the last both streptococci were absent...
January 11, 2018: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29326510/isolation-and-typing-of-streptococcus-mutans-and-streptococcus-sobrinus-from-caries-active-subjects
#7
Hamzah Abdulrahman Salman, R Senthilkumar, Khalid Imran, K Panneer Selvam
Background: Streptococcus mutans and Streptococcus sobrinus are main etiological agents of dental caries. Aim: The aim of the study was to isolate, identify, characterize, and determine the minimum inhibitory concentration (MIC) of S. mutans and S. sobrinus from caries-active subjects. Materials and Methods: Sixty-five plaque samples were collected from caries-active subjects aged between 35 and 44 years, processed and cultured on mitis salivarius bacitracin agar...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29323531/an-efficient-gas-chromatography-mass-spectrometry-approach-for-the-simultaneous-analysis-of-deoxynivalenol-and-its-bacterial-metabolites-3-keto-don-and-3-epi-don
#8
Kaijie Tang, Huaizhi Liu, Xiu-Zhen Li, Yousef I Hassan, Suqin Shao, Ting Zhou
Deoxynivalenol (DON) is one of the major toxic secondary metabolites produced by Fusarium fungi in cereal grains. Among the many promising strategies of DON detoxification are the microbial and enzymatic ones, which transform DON to nontoxic DON metabolites. Thus, proper analytical methods are needed for those DON metabolites. In this study, a robust gas chromatography-mass spectrometry (GC-MS) procedure was developed and validated for the simultaneous analysis of DON and two of its bacterial metabolites, 3-keto-DON and 3- epi-DON...
January 11, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29316223/probiotic-lactobacillus-sp-inhibit-growth-biofilm-formation-and-gene-expression-of-caries-inducing-streptococcus-mutans
#9
Reham Wasfi, Ola A Abd El-Rahman, Mai M Zafer, Hossam M Ashour
Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lacobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lacobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR)...
January 8, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29315011/bioluminescence-analysis-of-antibacterial-photodynamic-therapy-using-methylene-blue-mediated-by-low-intensity-level-laser-against-cariogenic-biofilms
#10
Fernando Luis Florez Esteban, Morgana Regina Mendonça de Oliveira, Osmir Batista de Oliveira Júnior, Rochelle Denise Hiers, Sharukh Soli Khajotia, Hermes Pretel
OBJECTIVE: A non-destructive and real-time bioluminescence (BL) assay was used to determine the utility of antimicrobial photodynamic therapy (aPDT) treatments mediated by methylene blue (MB) and laser irradiation (LI) against intact biofilms that are capable of producing caries (cariogenic). The efficacy of antibacterial photodynamic treatments has been currently determined by using either viable colony counts (VCC) or metabolic assays (Alamar Blue) that were demonstrated to have critical limitations when used on microcolony-forming bacteria such as Streptococcus mutans...
January 9, 2018: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29314344/caries-outcome-following-an-intensive-fluoride-varnish-treatment-regimen-for-children-at-high-risk-for-early-childhood-caries
#11
Agelina E Paek, Yihong Li, Zhemeng Wang, Patrick So, Malvin N Janal, Neal G Herman, Andrew Hopkins, Courtney Chinn
PURPOSE: To evaluate the caries relapse rate for a cohort of 2- to 4-year-old children at high risk of early childhood caries when treated with an intensive fluoride varnish (FV) regimen. METHODS: Eighty paediatric patients were recruited. Forty of these patients were high risk and received the FV treatment (three applications within 2 weeks and additional applications at 1 and 3 months) during 2009-2010. Mutans streptococci (MS) levels in the saliva were evaluated during the treatment period...
January 5, 2018: International Journal of Paediatric Dentistry
https://www.readbyqxmd.com/read/29310243/specific-chemiluminescent-protocol-for-dual-site-recognition-of-streptococcus-mutans-utilizing-strong-affinity-between-teicoplanin-and-gram-positive-bacteria
#12
Xiaoxiao Su, Mengyao Wang, Yue Wu, Yong He, Zhifeng Fu
A novel dual-site recognition protocol was developed for chemiluminescent (CL) detection of Streptococcus mutans (S. mutans) based on a designed antibiotic-affinity strategy. Teicoplanin, a broad-spectrum antibiotic against Gram-positive bacteria, was adopted to functionalize magnetic particles and recognize S. mutans utilizing the strong affinity between this agent and D-Alanyl-D-Alanine peptide moieties in the bacterial cell wall. To achieve ideal specificity for S. mutans detection, rat immunoglobulin G2a (rat IgG2a) tagged with horseradish peroxidase (HRP) was used as the second recognition agent and signal tracer since Fab region of rat IgG2a could bind with streptococcal protein G highly expressed in the cell wall of S...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29303754/surface-treatment-of-implant-materials-with-antimicrobial-nanoparticulates
#13
Nathalia Ferraz Oliscovicz, Denise Tornavoi de Castro, Mariana Lima da Costa Valente, Evandro Watanabe, César Penazzo Lepri, Andréa Cândido Dos Reis
This study analyzed nanostructured silver vanadate (AgVO3) films on certain substrate surfaces found in dental implant devices in order to see if these films promote antimicrobial activity. Enamel and glaze vehicles were used to apply the nanomaterial (0.0% [control], 2.5%, 5.0%, and 10.0% concentrations) on 3 substrates (280 specimens per substrate): polytetrafluoroethylene (PTFE), polyacetal, and acrylic resin. An agar diffusion method was performed in triplicate to determine the antimicrobial activity against 8 microorganisms...
January 2018: General Dentistry
https://www.readbyqxmd.com/read/29303123/comparative-evaluation-of-the-efficacy-of-tricalcium-phosphate-calcium-sodium-phosphosilicate-and-casein-phosphopeptide-amorphous-calcium-phosphate-in-reducing-streptococcus-mutans-levels-in-saliva
#14
V Samuel, M Ramakrishnan, H S Halawany, N B Abraham, V Jacob, S Anil
BACKGROUND: There are only limited studies that have determined the antibacterial effects of various remineralizing agents that can be beneficial to children. AIM: The aim of this study is to compare the efficacy of tricalcium phosphate (TCP), calcium sodium phosphosilicate (CSP), and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) enhanced with fluoride in reducing the Streptococcus mutans (SM) levels in saliva of children. MATERIALS AND METHODS: Out of 245 children, 120 of them with SM colony forming units (CFU)/ml in the range of 104-106/ml of saliva were assigned to four groups: (I) TCP; (II) CSP; (III) CPP-ACP enhanced with fluoride; and (IV) control...
November 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29296084/development-and-characterization-of-p1025-loaded-bioadhesive-liquid-crystalline-system-for-the-prevention-of-streptococcus-mutans-biofilms
#15
Giovana Maria Fioramonti Calixto, Cristiane Duque, Kelly Limi Aida, Vanessa Rodrigues Dos Santos, Loiane Massunari, Marlus Chorilli
Formation of a dental biofilm by Streptococcus mutans can cause dental caries, and remains a costly health problem worldwide. Recently, there has been a growing interest in the use of peptidic drugs, such as peptide p1025, analogous to the fragments 1025-1044 of S. mutans cellular adhesin, responsible for the adhesion and formation of dental biofilm. However, peptides have physicochemical characteristics that may affect their biological action, limiting their clinical performance. Therefore, drug-delivery systems, such as a bioadhesive liquid-crystalline system (LCS), may be attractive strategies for peptide delivery...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29284967/evaluation-of-antibacterial-activity-of-three-selected-fruit-juices-on-clinical-endodontic-bacterial-strains
#16
Subasish Behera, Prashant Khetrapal, Sandhya Kapoor Punia, Deepak Agrawal, Minal Khandelwal, Jitendra Lohar
Introduction: The increasing problem of antibiotic drug resistance by pathogenic microorganisms in the past few decades has recently led to the continuous exploration of natural plant products for new antibiotic agents. Many consumable food materials have good as well as their bad effects, good effect includes their antibacterial effects on different microorganisms present in the oral cavity. Recently, natural products have been evaluated as source of antimicrobial agent with efficacies against a variety of microorganisms...
November 2017: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/29284195/extracellular-matrix-influence-in-streptococcus-mutans-gene-expression-in-a-cariogenic-biofilm
#17
Elkin Jahir Florez Salamanca, Marlise Inêz Klein
Caries etiology is biofilm-diet dependent. Biofilms are highly dynamic and structured microbial communities enmeshed in a three-dimensional extracellular matrix. The study evaluated the expression dynamics of Streptococcus mutans genes associated with exopolysaccharides (EPS) (gtfBCD, gbpB, dexA), lipoteichoic acids (LTA) (dltABCD, SMU_775c) and extracellular DNA (eDNA) (lytST, lrgAB, ccpA) during matrix development within a mixed-species biofilm of S. mutans, Actinomyces naeslundii and Streptococcus gordonii...
December 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29282165/in-vitro-effects-of-sports-and-energy-drinks-on-streptococcus-mutans-biofilm-formation-and-metabolic-activity
#18
LaQuia A Vinson, Amy K Goodlett, Ruijie Huang, George J Eckert, Richard L Gregory
PURPOSE: Sports and energy drinks are being increasingly consumed and contain large amounts of sugars, which are known to increase Streptococcus mutans biofilm formation and metabolic activity. The purpose of this in vitro study was to investigate the effects of sports and energy drinks on S. mutans biofilm formation and metabolic activity. METHODS: S. mutans UA159 was cultured with and without a dilution (1:3 ratio) of a variety of sports and energy drinks in bacterial media for 24 hours...
September 15, 2017: Journal of Dentistry for Children
https://www.readbyqxmd.com/read/29279684/self-ligating-versus-conventional-metallic-brackets-on-streptococcus-mutans-retention-a-systematic-review
#19
Juliano N Longoni, Beatriz M Lopes, Irlan A Freires, Kamile L Dutra, Ademir Franco, Luiz R Paranhos
Objective: The present study aimed to review the literature systematically and assess comparatively whether self-ligating metallic brackets accumulate less Streptococcus mutans biofilm than conventional metallic brackets. Material and methods: The systematic search was performed following PRISMA guidelines and registration in PROSPERO. Seven electronic databases (Google Scholar, LILACS, Open Grey, PubMed, SciELO, ScienceDirect, and Scopus) were consulted until April 2016, with no restriction of language and time of publication...
October 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/29279621/calotropis-gigantea-extract-as-a-potential-anticariogenic-agents-against-streptococcus-mutans-an-in-vivo-comparative-evaluation
#20
Meenakshi Sharma, Sandeep Tandon, Ullal Anand Nayak, Damodhar Kappadi, Ambika Singh Rathore, Anil Goyal
Objective: The current study was intended to evaluate and compare the in vivo efficacy of Calotropis gigantea mouth rinse as anticariogenic agents with 0.2% chlorhexidine and Listerine mouth rinse. Materials and Methods: An experimental crossover study was conducted on sixty school children aged 14-15 years. Participants were divided into three Groups A, B, and C. In all children, baseline unstimulated saliva samples were collected and assessed for Streptococcus mutans counts...
May 2017: Journal of Conservative Dentistry: JCD
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