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https://www.readbyqxmd.com/read/28092483/photodynamic-inactivation-of-cariogenic-pathogens-using-curcumin-as-photosensitizer
#1
Natália Costa Araújo, Rebeca Ferraz de Menezes, Vanda Sanderana Macêdo Carneiro, Alexandrino Pereira Dos Santos-Neto, Carla Raquel Fontana, Vanderlei Salvador Bagnato, Catherine Malinda Harvey, Marleny Elizabeth Martinez Gerbi
OBJECTIVE: This investigation assessed the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to Photodynamic Therapy (PDT) when grown simultaneously in dentine carious lesions. BACKGROUND DATA: PDT is a technique that utilizes light to activate photosensitizers in the presence of oxygen to produce reactive radicals. MATERIALS AND METHODS: A culture medium of 1% glucose, 2% sucrose, 1% young primary culture of L. acidophilus 10(8) CFU/mL, and S...
January 12, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28087617/in-vitro-activity-of-the-antiasthmatic-drug-zafirlukast-against-the-oral-pathogens-porphyromonas-gingivalis-and-streptococcus-mutans
#2
Evelien Gerits, Isolde Van der Massen, Katleen Vandamme, Kaat De Cremer, Katrijn De Brucker, Karin Thevissen, Bruno P A Cammue, Serge Beullens, Maarten Fauvart, Natalie Verstraeten, Jan Michiels
Oral infections are among the most common diseases worldwide. Many protocols for the prevention and treatment of oral infections have been described, yet no golden standard has been developed so far. The antiseptic chlorhexidine and antibiotics are often used in these treatment procedures. However, long-term use of chlorhexidine can lead to side effects and extensive use of antibiotics can promote the development of antibiotic-resistant bacteria, which in turn can compromise the effectiveness of the treatment...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28081523/enhanced-enzymatic-hydrolysis-and-acetone-butanol-ethanol-fermentation-of-sugarcane-bagasse-by-combined-diluted-acid-with-oxidate-ammonolysis-pretreatment
#3
Hailong Li, Lian Xiong, Xuefang Chen, Can Wang, Gaoxiang Qi, Chao Huang, Mutan Luo, Xinde Chen
This study aims to propose a biorefinery pretreatment technology for the bioconversion of sugarcane bagasse (SB) into biofuels and N-fertilizers. Performance of diluted acid (DA), aqueous ammonia (AA), oxidate ammonolysis (OA) and the combined DA with AA or OA were compared in SB pretreatment by enzymatic hydrolysis, structural characterization and acetone-butanol-ethanol (ABE) fermentation. Results indicated that DA-OA pretreatment improves the digestibility of SB by sufficiently hydrolyzing hemicellulose into fermentable monosaccharides and oxidating lignin into soluble N-fertilizer with high nitrogen content (11...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28079378/dibenzofurans-and-pseudodepsidones-from-the-lichen-stereocaulon-paschale-collected-in-northern-quebec
#4
Claudia Carpentier, Emerson Ferreira Queiroz, Laurence Marcourt, Jean-Luc Wolfender, Jabrane Azelmat, Daniel Grenier, Stéphane Boudreau, Normand Voyer
Chemical investigation of the methanol extract of the lichen Stereocaulon paschale collected in Nunavik, Canada, led to the isolation and identification of two new dibenzofurans (1 and 3) and 11 known lichen metabolites. The structures of the new compounds were established by analysis of 1D and 2D NMR spectroscopic and high-resolution mass spectrometric data. Herein, the first isolation of ascomatic acid dibenzofuran derivatives (1-3) from a whole lichen organism is reported. In addition, some of the isolated metabolites showed antibacterial activity against the oral pathogens Porphyromonas gingivalis and Streptococcus mutans...
January 12, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28076651/controlled-release-of-chlorhexidine-from-a-mesoporous-silica-containing-macroporous-titanium-dental-implant-prevents-microbial-biofilm-formation
#5
K De Cremer, A Braem, E Gerits, K De Brucker, K Vandamme, J A Martens, J Michiels, J Vleugels, B P Cammue, K Thevissen
Roughened surfaces are increasingly being used for dental implant applications as the enlarged contact area improves bone cell anchorage, thereby facilitating osseointegration. However, the additional surface area also entails a higher risk for the development of biofilm associated infections, an etiologic factor for many dental ailments, including peri-implantitis. To overcome this problem, we designed a dental implant composed of a porous titanium-silica (Ti/SiO2) composite material and containing an internal reservoir that can be loaded with antimicrobial compounds...
January 11, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28076372/bad-to-the-bone-on-in-vitro-and-ex-vivo-microbial-biofilm-ability-to-directly-destroy-colonized-bone-surfaces-without-participation-of-host-immunity-or-osteoclastogenesis
#6
Adam Junka, Patrycja Szymczyk, Grzegorz Ziółkowski, Ewa Karuga-Kuzniewska, Danuta Smutnicka, Iwona Bil-Lula, Marzenna Bartoszewicz, Susan Mahabady, Parish Paymon Sedghizadeh
Bone infections are a significant public health burden associated with morbidity and mortality in patients. Microbial biofilm pathogens are the causative agents in chronic osteomyelitis. Research on the pathogenesis of osteomyelitis has focused on indirect bone destruction by host immune cells and cytokines secondary to microbial insult. Direct bone resorption by biofilm pathogens has not yet been seriously considered. In this study, common osteomyelitis pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Streptococcus mutans) were grown as biofilms in multiple in vitro and ex vivo experiments to analyze quantitative and qualitative aspects of bone destruction during infection...
2017: PloS One
https://www.readbyqxmd.com/read/28068269/chitosan-incorporated-in-a-total-etch-adhesive-system-antimicrobial-activity-against-streptococcus-mutans-and-lactobacillus-casei
#7
Marcelo Figueiredo Lobato, Cecilia Pedroso Turssi, Flávia Lucisano Botelho do Amaral, Fabiana Mantovani Gomes França, Roberta Tarkany Basting
The aim of this study was to investigate the antimicrobial effect of an experimental adhesive system containing chitosan (0.2% and 0.5%) against Streptococcus mutans and Lactobacillus casei. Twenty-four extracted human third molars were used, and 4 cavities were prepared in each tooth. The teeth were contaminated with either S mutans (n = 12 teeth, 48 cavities) or L casei (n = 12 teeth, 48 cavities) microorganisms. One cavity in each tooth received 1 of the following treatments: negative control (no treatment [NC]), positive control (Adper Single Bond 2 [SB]), an experimental adhesive containing chitosan 0...
January 2017: General Dentistry
https://www.readbyqxmd.com/read/28067778/quorum-sensing-regulation-of-competence-and-bacteriocins-in-streptococcus-pneumoniae-and-mutans
#8
REVIEW
Erin Shanker, Michael J Federle
The human pathogens Streptococcus pneumoniae and Streptococcus mutans have both evolved complex quorum sensing (QS) systems that regulate the production of bacteriocins and the entry into the competent state, a requirement for natural transformation. Natural transformation provides bacteria with a mechanism to repair damaged genes or as a source of new advantageous traits. In S. pneumoniae, the competence pathway is controlled by the two-component signal transduction pathway ComCDE, which directly regulates SigX, the alternative sigma factor required for the initiation into competence...
January 5, 2017: Genes
https://www.readbyqxmd.com/read/28067105/antimicrobial-effects-of-mastic-extract-against-oral-and-periodontal-pathogens
#9
Stefka Koychev, Henrik Dommisch, Hong Chen, Nicole Pischon
BACKGROUND: Various antimicrobial agents are widely used in the therapy of oral inflammatory diseases. However, their side effects and the appearance of drug resistance justify research on natural antimicrobial agents to target oral pathogens that are safe for the host. In the present study, antimicrobial properties of mastic extract on commensal and pathogenic oral bacteria, as well as its possible cytotoxic effect toward cells of epithelial and mesenchymal origin, were evaluated in comparison to the common antimicrobial agents hydrogen peroxide (H2O2) and chlorhexidine digluconate (CHX)...
January 8, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28066817/transcriptional-profiling-of-the-oral-pathogen-streptococcus-mutans-in-response-to-competence-signaling-peptide-xip
#10
Iwona B Wenderska, Andrew Latos, Benjamin Pruitt, Sara Palmer, Grace Spatafora, Dilani B Senadheera, Dennis G Cvitkovitch
In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S...
January 2017: MSystems
https://www.readbyqxmd.com/read/28053705/first-report-of-human-infection-due-to-streptococcus-devriesei
#11
P Tammaro, N Richard, B Andre, A Andremont, H Mammeri
So far, Streptococcus devriesei, which belongs to the mutans streptococci group, has been incriminated in the formation of caries in Equidae. We report the first human infection due to this species in a 54-year-old man with gangrenous cholecystitis. The patient was treated successfully by cholecystectomy and ceftriaxone.
January 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28050506/distribution-of-streptococcus-mutans-and-streptococcus-sobrinus-in-dental-plaque-of-indian-pre-school-children-using-pcr-and-sb-20m-agar-medium
#12
Divya Singla, Arun Sharma, Vinod Sachdev, Radhika Chopra
INTRODUCTION: Dental caries is one of the most common infectious diseases affecting the oral cavity. Among the oral bacteria, mutans streptococci have been implicated as major cariogenic bacteria as they can produce high levels of dental caries causing substances such as lactic acid and extracellular polysaccharides. AIM: The aim of the study was to detect the presence of Streptococcus mutans and Streptococcus sobrinus in dental plaque by using Polymerase Chain Reaction (PCR) method, quantification of these micro-organisms using Modified Sucrose-Bacitracin (SB-20M) agar medium and to correlate their presence in Caries Active (CA) and Caries Free (CF) pre-school children...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28050502/antibacterial-effect-of-aloe-vera-gel-against-oral-pathogens-an-in-vitro-study
#13
Supreet Jain, Nirav Rathod, Ravleen Nagi, Jaideep Sur, Afshan Laheji, Naveen Gupta, Priyanka Agrawal, Swati Prasad
INTRODUCTION: Natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. Aloe Vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties. AIM: The aim of the present study was to determine the anti-microbial and inhibitory activities of various concentration of Aloe Vera Gel (AVG) against oral pathogenic bacteria...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28050493/anti-microbial-efficacy-of-soursop-leaf-extract-annona-muricata-on-oral-pathogens-an-in-vitro-study
#14
Bh Mithun Pai, Gururagavendra Rajesh, Ramya Shenoy, Ashwini Rao
INTRODUCTION: Annona muricata also called as Soursop is a, flowering evergreen tree native to Mexico, Cuba, Central America and parts of India. The miracle tree as it is widely known as a natural cancer killer that is 10,000 times stronger than chemotherapy. Based on these miraculous claims, the leaves of these plants were used as an extract at varying concentrations as an antibacterial agent against oral pathogens. AIM: The aim of the study was to assess antimicrobial efficacy of Soursop leaf extarct (Annona muricata) on Streptococcus mutans, Streptococcus mitis, Porphyromonas gingivalis, Prevotella intermedia and Candida albicans using disc diffusion method...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28049966/five-year-longitudinal-study-of-dental-caries-risk-associated-with-streptococcus-mutans-and-streptococcus-sobrinus-in-individuals-with-intellectual-disabilities
#15
Yuki Oda, Fumiko Hayashi, Atsuko Wakita, Yukiko Nagatani, Mitsugi Okada
Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus) are important etiologic agents in human dental caries. Using quantitative real-time polymerase chain reaction assays for the presence of those strains, we examined 145 outpatients with intellectual disability (ID), calculated the proportion of each of these strains to total bacteria, and compared dental caries incidence over 5 years. Plaque samples were collected from all erupted tooth sites, and dental examinations were performed annually to determine numbers of decayed, missing, and filled teeth (DMFT score; World Health Organization caries diagnostic criteria)...
December 28, 2016: Journal of Oral Science
https://www.readbyqxmd.com/read/28045312/black-stains-a-microbiological-analysis-and-a-view-on-familiarity-and-susceptibility-to-tooth-decay-of-patients-in-childhood
#16
D Tripodi, D Martinelli, M Pasini, M R Giuca, S D'Ercole
AIM: Assess prevalence, familial predisposition and susceptibility to caries of Black Stains (BS). Evaluate the microbiological composition of BS, saliva and subgingival plaque. MATERIALS AND METHODS: Sixty nine subjects with BS (test group) and 120 subjects without BS (control group) were analysed for oral status. For each BS-patient, a BS-deposit, 1 ml of saliva and subgingival plaque were collected and microbiologically analysed. Five deciduous teeth with BS were observed under SEM...
December 2016: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28044252/antibacterial-activity-and-mechanism-of-ag-zno-nanocomposite-against-anaerobic-oral-pathogen-streptococcus-mutans
#17
Shilei Wang, Jie Wu, Hao Yang, Xiangyu Liu, Qiaomu Huang, Zhong Lu
Dental caries is a widespread disease mainly caused by the anaerobic oral pathogen Streptococcus mutans (S. mutans). Ag/ZnO nanocomposite is an efficient antibacterial agent because of its high antibacterial activity and low cytotoxicity. In this study, rod-like Ag/ZnO nanocomposite was synthesized through a deposition-precipitation method and characterized by X-ray diffraction, scanning electron microscopy and transmission electron microscopy. The activity of Ag/ZnO nanocomposite against S. mutans was evaluated by determining the minimal inhibitory concentration, minimum bactericidal concentration and growth inhibition curve...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28042275/the-level-of-beta-defensin-2-in-saliva-and-its-expression-in-parotid-gland-epithelial-cells-after-probiotic-lactobacillus-reuteri-induction-to-inhibit-streptococcus-mutans-in-caries
#18
Tuti Kusumaningsih, M S Subijanto, Retno Indrawati, R Rini Devijanti
OBJECTIVE: The aim of this study was to prove that administrating L. reuteri probiotics can increase the level of BD-2 saliva and BD-2 expression in the epithelial parotid glands of Wistar rats. MATERIALS AND METHODS: Experimental design in this study was randomized control group post test only. Twenty-four white male Rattus norvegicus Wistar strain rats were divided into four groups. The negative control group included rats not induced by S. mutans whereas the positive control group included rats induced by S...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28042266/a-self-disinfecting-irreversible-hydrocolloid-impression-material-mixed-with-povidone-iodine-powder
#19
Hussien Abdalfatah Ismail, Hani Asfour, Souaad Abdulelah Shikho
OBJECTIVES: The aim was to evaluate the effect of adding povidone (PVP) iodine powder with different concentrations to irreversible hydrocolloid on both microbiological and dimensional stability. MATERIALS AND METHODS: Regular set of (alginate) irreversible hydrocolloid was selected as control group. PVP-iodine powder was mixed with the alginate powder at concentrations of 1, 5, 10, 15, and 20% by weight (test groups). All specimens were tested for their antimicrobial effect against Streptococcus mutans and Staphylococcus aureus as well as dimensional stability...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28036052/sugar-allocation-to-metabolic-pathways-is-tightly-regulated-and-affects-the-virulence-of-streptococcus-mutans
#20
REVIEW
Miki Kawada-Matsuo, Yuichi Oogai, Hitoshi Komatsuzawa
Bacteria take up and metabolize sugar as a carbohydrate source for survival. Most bacteria can utilize many sugars, including glucose, sucrose, and galactose, as well as amino sugars, such as glucosamine and N-acetylglucosamine. After entering the cytoplasm, the sugars are mainly allocated to the glycolysis pathway (energy production) and to various bacterial component biosynthesis pathways, including the cell wall, nucleic acids and amino acids. Sugars are also utilized to produce several virulence factors, such as capsule and lipoteichoic acid...
December 28, 2016: Genes
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