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https://www.readbyqxmd.com/read/28448807/alternative-sweeteners-influence-the-biomass-of-oral-biofilm
#1
Fathilah Abdul Razak, Baizatul Amirah Baharuddin, Elisya Farha Mohd Akbar, Amira Hanim Norizan, Nur Fazilah Ibrahim, Md Yusoff Musa
OBJECTIVE: Compact-structured oral biofilm accumulates acids that upon prolonged exposure to tooth surface, causes demineralisation of enamel. This study aimed to assess the effect of alternative sweeteners Equal Stevia(®), Tropicana Slim(®), Pal Sweet(®) and xylitol on the matrix-forming activity of plaque biofilm at both the early and established stages of formation. METHODS: Saliva-coated glass beads (sGB) were used as substratum for the adhesion of a mixed-bacterial suspension of Streptococcus mutans, Streptococcus sanguinis and Streptococcus mitis...
April 20, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28440891/evaluation-of-three-different-decontamination-techniques-on-biofilm-formation-and-on-physical-and-chemical-properties-of-resin-composites
#2
Carolina Bosso André, Andressa Dos Santos, Carmem Silvia Pfeifer, Marcelo Giannini, Emerson Marcelo Girotto, Jack Liborio Ferracane
OBJECTIVES: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. METHODS: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans...
April 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28438559/the-role-of-copper-nanoparticles-in-an-etch-and-rinse-adhesive-on-antimicrobial-activity-mechanical-properties-and-the-durability-of-resin-dentine-interfaces
#3
Mario F Gutiérrez, Pamela Malaquias, Viviane Hass, Thalita P Matos, Lucas Lourenço, Alessandra Reis, Alessandro D Loguercio, Paulo Vitor Farago
OBJECTIVES: To evaluate the effect of addition of copper nanoparticles at different concentrations into an etch-and-rinse adhesive (ER) on antimicrobial activity, Knoop microhardness (KHN), in vitro and in situ degree of conversion (DC), as well as the immediate (IM) and 2-year (2Y) resin-dentine bond strength (μTBS) and nanoleakage (NL). METHODS: Seven experimental ER adhesives were formulated according to the amount of copper nanoparticles incorporated into the adhesives (0 [control], 0...
April 21, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28435894/oral-bacterial-acid-base-metabolism-in-caries-screening-a-proof-of-concept-study
#4
E Morou-Bermudez, M A Loza-Herrero, V Garcia-Rivas, E Suarez-Perez, R J Billings
The objective of this cross-sectional study was to clinically validate an array of biochemical tests for oral acid/alkali generation as caries screening instruments. 185 adult subjects (mean 33.6±10.6 years) were examined clinically for dental caries using the ICDAS criteria. Bitewing radiographs were used to confirm interproximal surfaces of posterior teeth. For the purposes of this study, subjects were classified as "caries-active" if they had at least one untreated caries lesion with ICDAS 4 or higher. Pooled supragingival plaque and unstimulated saliva samples were collected and assayed for pH changes from sucrose and urea metabolism using colorimetric tests...
April 2017: JDR Clinical and Translational Research
https://www.readbyqxmd.com/read/28435376/comparative-evaluation-of-antimicrobial-efficacy-of-an-alternative-natural-agent-for-disinfection-of-toothbrushes
#5
R Vignesh, C Vishnu Rekha, Parisa Norouzi Baghkomeh, Sankar Annamalai, Ditto Sharmin
OBJECTIVE: The aim of this study was to assess the antimicrobial potency of aqueous extract of Psidium guajava leaves in two different concentrations as a toothbrush disinfectant against three oral bacterial species. MATERIALS AND METHODS: Aqueous extracts of P. guajava leaves were prepared at 20% and 30% concentrations and 0.2% chlorhexidine was used as control. The toothbrushes were equally divided into 9 groups with 10 toothbrushes per disinfectant, which were contaminated with Streptococcus mutans, Lactobacillus acidophilus, and Enterococcus faecalis...
January 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28435253/antimicrobial-photodynamic-activity-and-cytocompatibility-of-au25-capt-18-clusters-photoexcited-by-blue-led-light-irradiation
#6
Saori Miyata, Hirofumi Miyaji, Hideya Kawasaki, Masaki Yamamoto, Erika Nishida, Hiroko Takita, Tsukasa Akasaka, Natsumi Ushijima, Toshihiko Iwanaga, Tsutomu Sugaya
Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen ((1)O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. (1)O2 generation of Au25(Capt)18 clusters under blue LED irradiation (420-460 nm) was detected by a methotrexate (MTX) probe...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28422601/degradation-of-resin-restorative-materials-by-streptococcus-mutans-a-pilot-study
#7
Ankit K Gautam, Ruchi Thakur, N D Shashikiran, Singla Shilpy, Nikita Agarwal, Shilpi Tiwari
OBJECTIVE: To evaluate the degradation of three resin based restorative materials by S Mutans. STUDY DESIGN: Class I cavity was prepared in extracted premolars and were randomly divided into 3 groups (Group I - Conventional composite (CC), Group II - Resin Modified GIC and Group III-Giomer). Teeth were then restored by respective restorative material and equally divided in two subgroups (Control and Experimental). Experiment subgroup samples were then incubated in 2 ml of BHI with 1:10 dilution of SM (MTCC-497) grown overnight in BHI whereas control subgroup samples were incubated in BHI without SM...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28420834/improving-the-efficacy-of-chlorhexidine-releasing-elastomerics-using-a-layerby-layer-coating-technique
#8
Hyun-Sun Jeon, Eun-Ha Jung, Si-Mook Kang, Eun-Song Lee, Jeong-Woo Lee, Baek-Il Kim
The aims of this study were to identify the optimal concentration of coated orthodontic elastomerics using a layer-by-layer technique that can release chlorhexidine (CHX) as an antimicrobial material, and to measure the physical properties and antimicrobial effects of the coated elastomerics. Ethyl cellulose (EC) was used as the polymer, and five study groups with various combinations of solvents (i.e., ethanol and dichloromethane [DCM]) were included. The coated elastomerics were evaluated with a spectrophotometer to confirm the release of CHX, and their surfaces were observed by SEM...
April 18, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28415495/silver-sulfadiazine-immobilized-glass-as-antimicrobial-fillers-for-dental-restorative-materials
#9
Rohit Srivastava, Yuyu Sun
Bacterial colonization and biofilm formation on dental resin composites cause secondary caries that shortens the service life of dental restorative materials. In this study, commercial barium borosilicate based glass powders, fillers widely used in dental composites, were covalently immobilized with silver sulfadiazine to provide antimicrobial effects. The chemical reactions were followed by FT-IR study, elemental analysis, and energy dispersive spectroscopy (EDS), and the reaction pathway was confirmed by model compound studies...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415444/formulation-of-thermoreversible-gel-of-cranberry-juice-concentrate-evaluation-biocompatibility-studies-and-its-antimicrobial-activity-against-periodontal-pathogens
#10
Rajeshwari H R, Dinesh Dhamecha, Satveer Jagwani, Dhanashree Patil, Satisha Hegde, Raksha Potdar, Renuka Metgud, Sunil Jalalpure, Subarna Roy, Kiran Jadhav, Neeraj Kumar Tiwari, Sravani Koduru, Shweta Hugar, Suneel Dodamani
The present work aims to investigate the efficacy of thermoreversible gel of cranberry juice concentrate (CJC) as local drug delivery for the treatment of periodontitis. CJC was initially tested for its antimicrobial activities like MIC, MBC, antiadhesion, antibiofilm and time kill assay against the panel of organisms (S. mutans (SM), E. faecalis (EF), A. actinomycetemcomitans (AA), P. gingivalis (PG), T. forsythia (TF)) responsible for periapical and periodontal infections. Antimicrobial activity of CJC showed MIC value of 50mg/ml and MBC value of 100mg/ml with desirable antiadhesion (83-90%) and antibiofilm activity (70-85%)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415415/curcumin-loaded-polysaccharide-nanoparticles-optimization-and-anticariogenic-activity-against-streptococcus-mutans
#11
Amir Maghsoudi, Fatemeh Yazdian, Saleheh Shahmoradi, Leila Ghaderi, Mehran Hemati, Ghassem Amoabediny
Curcumin was loaded into different polysaccharide nanoparticles chitosan, alginate and starch, using the desolvation method. Curcumin-loaded nanoparticles exhibited enhanced solubility in aqueous solutions comparing with free curcumin. Effects of formulation parameters such as curcumin concentration and different volumes of ethanolic solution were affected the particle size and loading efficiency. Under optimum conditions, curcumin-loaded chitosan, starch and alginate nanoparticles with mean particles sizes of 66...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414082/antimicrobial-photodynamic-therapy-on-streptococcus-mutans-is-altered-by-glucose-in-the-presence-of-methylene-blue-and-red-led
#12
Cintia R Lima Leal, Letícia H Alvarenga, Tamires Oliveira-Silva, Ilka T Kato, Bianca Godoy-Miranda, Sandra K Bussadori, Martha S Ribeiro, Renato A Prates
BACKGROUND: Dental caries are multifactorial disease and progressively produces tooth destruction as result from bacterial colonization of enamel surface, especially Streptococcus mutans. The objective of this work was to investigate the role of glucose in antimicrobial photodynamic therapy (aPDT) on S. mutans. METHODS: S. mutans ATCC 25175 were cultured on microaerophilia at 37°C for 48h, and we tested aPDT in the presence of 50mM glucose. Bacterial suspension was used to investigate aPDT with 100μM methylene blue (MB) under LED emitting radiation at ʎ=660nm and parameters as following (P=473 mW; I=166...
April 13, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28412792/-isolation-of-cariogenic-streptococcus-mutans-and-the-inhibitory-effect-of-egg-yolk-antibody-on-caries
#13
X L Li, Z Zhang, Z X Li, N J Deng, B Zeng, Y M Chen
Objective: To isolate the cariogenic Streptococcus mutans (Sm) strains and study the therapeutical effect of egg yolk antibody (IgY) of the Sm on dental caries development. Methods: Sm strains were isolated from the children's dental plaque samples. Morphological, biochemical and molecular biological methods were applied to identify the serotype, acid producing and adhesion abilities of isolated Sm strains. After inactivation one of the Sm strains was used as antigen to immune laying hens to collect and extract the specific anti-Sm IgY...
April 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/28407906/antioxidant-and-antibiofilm-activities-of-secondary-metabolites-from-ziziphus-jujuba-leaves-used-for-infusion-preparation
#14
Silvia Damiano, Martino Forino, Arpan De, Luca A Vitali, Giulio Lupidi, Orazio Taglialatela-Scafati
Ziziphus jujuba Mill., jujube, leaf infusions are popular bedtime beverages as they improve sleep by soothing the nerves. With the aim of providing further insights into the polyphenol content of jujube leaf infusion and their antioxidant activities, a 2,2-diphenyl-1-picrylhydrazyl (DPPH)-guided purification of the aqueous extract of Z. jujuba Mill. leaves was conducted. Three major antioxidant polyphenols (i.e. quercitrin, catechin and gallocatechin) of the leaves were identified. In addition, a significant antibiofilm bioactivity against Streptococcus mutans, a causative agent of human dental caries, was detected for the aqueous leaf extract and its bio-guided separation resulted in the identification of the lupane triterpenoid alphitolic acid as the main antibiofilm metabolite...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28403648/consumption-of-apple-boysenberry-beverage-decreases-salivary-actinomyces-naeslundii-and-their-adhesion-in-a-multi-species-biofilm-model
#15
S G Parkar, S Eady, M Cabecinha, M A Skinner
We hypothesised that consumption of beverage rich in both fibre and polyphenols, rather than each bioactive alone, will modulate populations of selected salivary bacteria, and their adhesion characteristics and that some of these effects may be due to the anti-microbial activity of the beverage bioactives. We investigated the effect of 4 weeks' consumption of beverages, rich in apple fibre, boysenberry polyphenols, or both on salivary bacteria in healthy subjects. In this placebo-controlled crossover study, saliva samples were collected at the beginning and end of each treatment period, and used for qPCR quantitation of Lactobacillus spp...
April 13, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28403360/influence-of-resin-modified-glass-ionomer-and-topical-fluoride-on-levels-of-streptococcus-mutans-in-saliva-and-biofilm-adjacent-to-metallic-brackets
#16
Marcela Cristina Damião Andrucioli, Gisele Faria, Paulo Nelson-Filho, Fábio Lourenço Romano, Mírian Aiko Nakane Matsumoto
Decalcification of enamel during fixed orthodontic appliance treatment remains a problem. White spot lesions are observed in nearly 50% of patients undergoing orthodontic treatment. The use of fluoride-containing orthodontic materials has shown inconclusive results on their ability to reduce decalcification. The aims of this investigation were to compare the levels of Streptococcus mutans (SM) in saliva and biofilm adjacent to orthodontic brackets retained with a resin-modified glass ionomer cement (RMGIC) (Fuji ORTHO LC) and a light cured composite resin (Transbond XT), and to analyze the influence of topical application of the 1...
March 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28401466/antibacterial-effects-of-lactobacillus-isolates-of-curd-and-human-milk-origin-against-food-borne-and-human-pathogens
#17
Chetan Sharma, Brij Pal Singh, Nishchal Thakur, Sachin Gulati, Sanjolly Gupta, Santosh Kumar Mishra, Harsh Panwar
This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei, L. delbrueckii, L. fermentum, L. plantarum, and L. pentosus. Antibacterial activities of cell-free supernatants (CFSs) of all the Lactobacillus isolates were estimated through standard agar-well diffusion assay, against commonly occurring food-borne and clinically important human pathogens...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28401067/a-nuclease-from-streptococcus-mutans-facilitates-biofilm-dispersal-and-escape-from-killing-by-neutrophil-extracellular-traps
#18
Jia Liu, Luping Sun, Wei Liu, Lihong Guo, Zhaohui Liu, Xi Wei, Junqi Ling
Streptococcus mutans is the primary etiologic agent of dental caries and occasionally infective endocarditis, with the ability to form biofilms and disperse cells into distal sites to exacerbate and spread infection. In this study, we identified a nuclease (DeoC) as a S. mutans biofilm dispersal modulating factor through microarray analysis. In vitro assays revealed a dispersal defect of a deoC deletion mutant, and functional studies with purified protein were indicative of the biofilm dispersal activity of DeoC...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28397552/in-vitro-antimicrobial-activity-of-thymus-vulgaris-essential-oil-against-major-oral-pathogens
#19
Mohammadmehdi Fani, Jamshid Kohanteb
The objective of present investigation was to determine antimicrobial activity of Thymus vulgaris oil on some oral pathogens. Thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of Streptococcus pyogenes, Streptococcus mutans, Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. Thymus vulgaris oil at concentrations of 16 to 256 μg/mL exhibited strong inhibitory activity on all clinical isolates producing inhibition zones of 7...
January 1, 2017: Journal of Evidence-based Complementary & Alternative Medicine
https://www.readbyqxmd.com/read/28394940/streptococcal-adhesin-sspa-b-analogue-peptide-inhibits-adherence-and-impacts-biofilm-formation-of-streptococcus-mutans
#20
Tatsuro Ito, Takahiro Ichinosawa, Takehiko Shimizu
Streptococcus mutans, the major causative agent of dental caries, adheres to tooth surfaces via the host salivary glycoprotein-340 (gp340). This adherence can be competitively inhibited by peptides derived from the SspA/B adhesins of Streptococcus gordonii, a human commensal microbe that competes for the same binding sites. Ssp(A4K-A11K), a double-lysine substituted SspA/B peptide analogue, has been shown to exhibit superior in vitro binding affinity for a gp340-derived peptide (SRCRP2), suggesting that Ssp(A4K-A11K) may be of clinical interest...
2017: PloS One
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