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https://www.readbyqxmd.com/read/29775904/biosynthesis-of-chiral-epichlorohydrin-using-an-immobilized-halohydrin-dehalogenase-in-aqueous-and-non-aqueous-phase
#1
Xiao-Jian Zhang, Ping-Xiu Shi, Han-Zhong Deng, Xin-Xin Wang, Zhi-Qiang Liu, Yu-Guo Zheng
Asymmetric synthesis of chiral epichlorohydrin (ECH) from 1,3-dichloro-2-propanol (1,3-DCP) using halohydrin dehalogenase (HHDH) is of great value due to the 100% theoretical yield and high enantioselectivity. In this study, HheC (P175S/W249P) was immobilized on an A502Ps resin and used for the preparation of (S)-ECH. In aqueous system, the immobilized HheC catalyzed the biosynthesis of (S)-ECH with 83.78% yield and 92.53% enantiomeric excess (ee) at 1,3-DCP concentration of 20 mM. The non-aqueous system was further developed using water saturated ethyl acetate as solvent and reaction phase...
May 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29774107/clinical-outcome-of-hepatocellular-carcinoma-can-be-predicted-by-the-expression-of-hepatic-progenitor-cell-markers-and-serum-tumour-markers
#2
Satoshi Seino, Atsunori Tsuchiya, Yusuke Watanabe, Yuzo Kawata, Yuichi Kojima, Shunzo Ikarashi, Hiroyuki Yanai, Koji Nakamura, Daisuke Kumaki, Masaaki Hirano, Kazuhiro Funakoshi, Takashi Aono, Takeshi Sakai, Jun Sakata, Masaaki Takamura, Hirokazu Kawai, Satoshi Yamagiwa, Toshifumi Wakai, Shuji Terai
The high heterogeneity of hepatocellular carcinomas (HCCs) complicates stratification of HCC patients for treatment. Therefore, it is necessary to establish a comprehensive panel of HCC biomarkers related to tumour behaviour and cancer prognosis. Resected HCCs from 251 patients were stained for hepatic progenitor cell (HPC) markers epithelial cell adhesion molecule (EpCAM), neural cell adhesion molecule (NCAM), delta-like 1 homolog (DLK1), and cytokeratin 19 (CK19). Staining patterns were analysed for their prognostic association with relapse-free survival and overall survival...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29773401/optimization-of-orodispersible-and-conventional-tablets-using-simplex-lattice-design-relationship-among-excipients-and-banana-extract
#3
Sureewan Duangjit, Pakorn Kraisit
The objective of this study was focused on the optimization of the pharmaceutical excipients and banana extract in the preparation of orally disintegrating banana extract tablets (OD-BET) and conventional banana extract tablets (CO-BET) using a simplex lattice design. Various ratios of banana extract (BE), dibasic calcium phosphate (DCP) and microcrystalline cellulose (MCC) were used to prepare banana extract tablets (BET). The results indicated that the optimal OD-BET and CO-BET consisted of BE: DCP: MCC at 10...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29757468/treatment-of-class-iii-multiple-gingival-recessions-prognostic-factors-for-achieving-a-complete-root-coverage
#4
Sofia Aroca, Antoine Barbieri, Marco Clementini, Franck Renouard, Massimo de Sanctis
BACKGROUND: This report is intended to present a supplemental analysis of data from a prior report (Aroca 2010) to investigate factors associated with a complete root coverage at 1 year. The purpose of the prior report was to investigate at 1 year the adjunction effect of EMD for the treatment of Miller's class III recession defects using a coronally advanced modified tunnel/CTG technique with (test group) or without (control group). The purpose of this report is to investigate additional factors associated with root coverage in the same data set...
May 14, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29752350/the-domino-swi2-snf2-gene-product-represses-cell-death-in-drosophila-melanogaster
#5
Kaitlyn Ellis, Joanna Wardwell-Ozgo, Kenneth H Moberg, Barry Yedvobnick
The Drosophila domino locus encodes DNA-dependent ATPases of the SWI2/SNF2 class. This class of chromatin remodeler is associated with an array of cellular activities encompassing transcription, replication, repair and recombination. Moreover, domino was observed initially to maintain a repressive chromatin state via genetic interaction studies with homeotic genes. Although domino mutations were also characterized with a cell death phenotype, its association with a death pathway has not been investigated. Here we have used targeted RNA interference to depress domino function in the wing...
May 11, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29748751/in-situ-generation-of-hydroxyl-radical-for-efficient-degradation-of-2-4-dichlorophenol-from-aqueous-solutions
#6
Saeid Ahmadzadeh, Maryam Dolatabadi
Since 2,4-dichlorophenol (2,4-DCP) as a priority pollutant is used in numerous industrial processes, its removal from the aqueous environment is of utmost importance and desire. Herein, the authors describe an electrochemical treatment process for efficient removal of 2,4-DCP from aqueous solutions using electro-Fenton (EF) process. Response surface methodology (RSM) was applied to optimize the operating parameters. Analysis of variance (ANOVA) confirmed the significance of the predicted model. The effect of independent variables on the removal of 2,4-DCP was investigated and the best removal efficiency of 98...
May 10, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29748719/electrochemical-determination-of-2-4-dichlorophenol-by-using-a-glassy-carbon-electrode-modified-with-molybdenum-disulfide-ionic-liquid-and-gold-silver-nanorods
#7
Huayu Huang, Mingxia Wang, Yang Wang, Xinli Li, Zhiying Niu, Xingyu Wang, Jinxi Song
Molybdenum disulfide (MoS2 ) was used as an electrically conductive skeleton and functionalized with an ionic liquid and gold/silver nanorods. The resulting composite was characterized by scanning electron microscopy, transmission electron microscopy and UV-vis spectroscopy. The composites were used to modify a glassy carbon electrode (GCE) to obtain a sensor for 2,4-dichlorophenol (2,4-DCP). The results show that the oxidation power and electrocatalytic activity of the modified GCE towards 2,4-DCP are enhanced compared to a bare GCE and other modified GCEs...
May 10, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29738906/mechanism-for-removing-2-4-dichlorophenol-via-adsorption-and-fenton-like-oxidation-using-iron-based-nanoparticles
#8
Li Gan, Beibei Li, Mengyu Guo, Xiulan Weng, Ting Wang, Zuliang Chen
In this paper, iron-based nanoparticles (Fe NPs) synthesized by Euphorbia cochinchensis leaf extract were used to remove 2,4-dichlorophenol (2,4-DCP). The possible mechanism for removing the adsorption and heterogeneous Fenton-like oxidation of 2,4-DCP was investigated. Various parameters affecting removal efficiency were tested, and the results showed that more than 83.5% of 2,4-DCP was removed with the addition of 10 mM H2 O2 and the initial concentration of 2,4-DCP of 30 mg/L at pH 6.26 under 303 K...
April 30, 2018: Chemosphere
https://www.readbyqxmd.com/read/29728082/influence-of-denture-surface-roughness-and-host-factors-on-dental-calculi-formation-on-dentures-a-cross-sectional-study
#9
Keisuke Matsumura, Yuji Sato, Noboru Kitagawa, Toshiharu Shichita, Daisuke Kawata, Mariko Ishikawa
BACKGROUND: Dental calculi formation on dentures can worsen the oral cavity environment by complicating oral hygiene. However, few studies have investigated the effect of how patients use and manage their dentures, denture surface roughness, and host factors such as oral cavity dryness and saliva properties on denture cleanliness and denture dental calculi formation. Accordingly, we conducted the present survey to evaluate these factors to clarify the strength of the influence of each factor...
May 4, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29723751/new-insights-for-risks-of-chlorophenols-cps-exposure-inhibition-of-udp-glucuronosyltransferases-ugts
#10
Kai Yang, Zhi-Wei Fu, Yun-Feng Cao, Sai-Nan Li, Zuo Du, Xiao-Yu Sun, Yong-Zhe Liu, Kun Yang, Zhong-Ze Fang
Chlorophenols (CPs) are important pollutants extensively utilized in industry, agriculture and forestry. The present study aims to determine the inhibition of CPs on the activity of the important phase II drug-metabolizing enzymes (DMEs) UDP-glucuronosyltransferases (UGTs). 100 μM of fourteen CPs were used for preliminary screening using in vitro incubation. Furthermore, half inhibition concentration (IC50 ) and inhibition kinetics were determined for CPs with significant inhibition towards UGT isoforms...
April 24, 2018: Chemosphere
https://www.readbyqxmd.com/read/29719311/registry-data-coordinator-rdc-a-proper-accessible-strategy-for-improving-road-traffic-injury-rti-hospital-based-trauma-registry-systems-in-developing-countries-and-low-income-countries
#11
Zahra Meidani, Mehrdad Mahdian, Atefe Ayan, Mahdi Mohammadzade, Alimohammad Nickfarjam, Gholam Abbas Moosavi
Introduction: Evidence suggested that a significant level of trauma mortality can be prevented using registry system. Aim: This study aimed to improve Kashan Hospital Based Trauma Registry System (KHBTRS) for Road Traffic Injury (RTI). Material and methods: After conducting focus group discussion absence of minimum data set (MDS) and poor data collection process (DCP) were identified as main problems for KHBTRS- RTI. Proposed MDS were surveyed by 20 experts of trauma research center of throughout the Iran...
2018: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/29706912/the-diaper-change-play-validation-of-a-new-observational-assessment-tool-for-early-triadic-family-interactions-in-the-first-month-postpartum
#12
Jérôme Rime, Hervé Tissot, Nicolas Favez, Michael Watson, Werner Stadlmayr
The quality of family relations, observed during mother-father-infant triadic interactions, has been shown to be an important contributor to child social and affective development, beyond the quality of dyadic mother-child, father-child, and marital relationships. Triadic interactions have been well described in families with 3 month olds and older children using the Lausanne Trilogue Play (LTP). Little is known about the development of mother-father-baby interactions in the very 1st weeks postpartum, mostly because no specific observational setting or particular instrument had been designed to cover this age yet...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/29704805/advanced-data-mining-approaches-in-the-assessment-of-urinary-concentrations-of-bisphenols-chlorophenols-parabens-and-benzophenones-in-brazilian-children-and-their-association-to-dna-damage
#13
Bruno A Rocha, Alexandros G Asimakopoulos, Masato Honda, Nattane L da Costa, Rommel M Barbosa, Fernando Barbosa, Kurunthachalam Kannan
Human exposure to endocrine disrupting chemicals (EDCs) has received considerable attention over the last three decades. However, little is known about the influence of co-exposure to multiple EDCs on effect-biomarkers such as oxidative stress in Brazilian children. In this study, concentrations of 40 EDCs were determined in urine samples collected from 300 Brazilian children of ages 6-14 years and data were analyzed by advanced data mining techniques. Oxidative DNA damage was evaluated from the urinary concentrations of 8-hydroxy-2'-deoxyguanosine (8OHDG)...
April 25, 2018: Environment International
https://www.readbyqxmd.com/read/29703899/mitochondria-targeted-probes-for-imaging-protein-sulfenylation
#14
Reetta J Holmila, Stephen A Vance, Xiaofei Chen, Hanzhi Wu, Kirtikar Shukla, Manish S Bharadwaj, Jade Mims, Zack Wary, Glen Marrs, Ravi Singh, Anthony J Molina, Leslie B Poole, S Bruce King, Cristina M Furdui
Mitochondrial reactive oxygen species (ROS) are essential regulators of cellular signaling, metabolism and epigenetics underlying the pathophysiology of numerous diseases. Despite the critical function of redox regulation in mitochondria, currently there are limited methods available to monitor protein oxidation in this key subcellular organelle. Here, we describe compounds for imaging sulfenylated proteins in mitochondria: DCP-NEt2 -Coumarin (DCP-NEt2 C) and rhodamine-based DCP-Rho1. Side-by-side comparison studies are presented on the reactivity of DCP-NEt2 C and DCP-Rho1 with a model protein sulfenic acid (AhpC-SOH) and mitochondrial localization to identify optimized experimental conditions for labeling and visualization of protein sulfenylation that would be independent of mitochondria membrane potential and would not impact mitochondrial function...
April 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29698914/error-quantification-of-osteometric-data-in-forensic-anthropology
#15
Natalie R Langley, Lee Meadows Jantz, Shauna McNulty, Heli Maijanen, Stephen D Ousley, Richard L Jantz
This study evaluates the reliability of osteometric data commonly used in forensic case analyses, with specific reference to the measurements in Data Collection Procedures 2.0 (DCP 2.0). Four observers took a set of 99 measurements four times on a sample of 50 skeletons (each measurement was taken 200 times by each observer). Two-way mixed ANOVAs and repeated measures ANOVAs with pairwise comparisons were used to examine interobserver (between-subjects) and intraobserver (within-subjects) variability. Relative technical error of measurement (TEM) was calculated for measurements with significant ANOVA results to examine the error among a single observer repeating a measurement multiple times (e...
April 10, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29689026/optical-coherence-tomography-angiography-findings-in-patients-with-nonocular-beh%C3%A3-et-disease
#16
Karim A Raafat, Riham S H M Allam, Basma M Medhat
PURPOSE: To describe the optical coherence tomography angiography findings in nonocular Behçet disease. METHODS: The superficial capillary plexus (SCP) and deep capillary plexus (DCP) and outer retinal and choroidal flow were evaluated using optical coherence tomography angiography. Perimetry was performed to correlate any microvascular and functional changes. RESULTS: Capillary nonperfusion areas were found in the superficial capillary plexus in 16/20 eyes (80%) and in the DCP in 17/20 eyes (85%)...
April 23, 2018: Retina
https://www.readbyqxmd.com/read/29678309/photo-fenton-degradation-of-a-herbicide-2-4-d-in-groundwater-for-conditions-of-natural-ph-and-presence-of-inorganic-anions
#17
Leandro O Conte, Agustina V Schenone, Bárbara N Giménez, Orlando M Alfano
The effects of four inorganic anions (Cl- , SO4 2 -, HCO3 - , NO3 - ) usually present in groundwater were investigated on the photo-Fenton degradation of 2,4-dichlorophenoxyacetic acid (2,4-D). A kinetic model derived from a reaction sequence is proposed using the ferrioxalate complex as iron source at pH close to natural conditions (pH = 5). It was demonstrated that oxalate not only maintained iron in solution for the natural groundwater system, but also increased the photochemical activation of the process...
April 5, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29674147/degradation-of-2-4-dichlorophenol-in-aqueous-solution-by-dielectric-barrier-discharge-effects-of-plasma-working-gases-degradation-pathways-and-toxicity-assessment
#18
Hong Zhang, Qifu Zhang, Chunguang Miao, Qing Huang
Chlorinated phenols are a class of contaminants found in water and have been regarded as a great potential risk to environment and human health. It is thus urgent to develop effective techniques to remove chlorinated phenols in wastewater. For this purpose, we employed dielectric barrier discharge (DBD) in this work and studied the efficiency of DBD for the degradation of 2,4-dichlorophenol (2,4-DCP), one of the most typical chlorophenols in the environment. The effects of pH value, applied voltage and plasma-working gases on the dichlorophenol-removal efficiency were investigated...
April 11, 2018: Chemosphere
https://www.readbyqxmd.com/read/29672690/infections-and-associated-costs-following-cardiovascular-implantable-electronic-device-implantations-a-nationwide-cohort-study
#19
Nicolas Clémenty, Phuong Lien Carion, Lucie de Léotoing, Ludovic Lamarsalle, Fanny Wilquin-Bequet, Benedict Brown, Koen J P Verhees, Jérôme Fernandes, Jean-Claude Deharo
Aims: This study assessed the contemporary occurrence of cardiac device infections (CDIs) following implantation in French hospitals and estimated associated costs. Methods and Results: A retrospective analysis was conducted on the French National Hospital Database (PMSI). Patients with a record of de novo cardiac implantable electronic device (CIED) implantation or replacement interventions in France in 2012 were identified and followed until the end of 2015. Cardiac device infections (CDIs) were identified based on coding using the French classification for procedures [Classification Commune des Actes Médicaux (CCAM)] and International Classification of Diseases (ICD-10)...
April 17, 2018: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/29655736/the-molecularly-imprinted-polymer-supported-by-anodic-alumina-oxide-nanotubes-membrane-for-efficient-recognition-of-chloropropanols-in-vegetable-oils
#20
Xiuzhen Qiu, Xian-Yan Xu, Yong Liang, Huishi Guo
A new route to synthesize a covalent interaction-based molecularly imprinted polymer (MIP) material for 3-chloro-1,2-propanediol (3-MCPD) inside the nanopores of anodic alumina oxide (AAO) is presented. A series of adsorption experiments showed MIP had good extraction capacity and selectivity for 3-MCPD. In order to evaluate the usability of the MIP nanotubes membrane, a method combining AAO@MIP membrane extraction with gas chromatography - mass spectrometry (GC-MS) detection was developed for determination of chloropropanols...
August 30, 2018: Food Chemistry
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