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Platelet derived microparticle

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https://www.readbyqxmd.com/read/29330877/characterization-of-biologic-response-modifiers-in-the-supernatant-of-conventional-refrigerated-and-cryopreserved-platelets
#1
Lacey Johnson, Shereen Tan, Emily Jenkins, Ben Wood, Denese C Marks
BACKGROUND: Alternatives to room temperature storage of platelets (PLTs) are of interest to support blood banking logistics. The aim of this study was to compare the presence of biologic response modifiers (BRMs) in PLT concentrates stored under conventional room temperature conditions with refrigerated or cryopreserved PLTs. STUDY DESIGN AND METHODS: A three-arm pool-and-split study was carried out using buffy coat-derived PLTs stored in 30% plasma/70% SSP+. The three matched treatment arms were as follows: room temperature (20-24°C), cold (2-6°C), and cryopreserved (-80°C with DMSO)...
January 12, 2018: Transfusion
https://www.readbyqxmd.com/read/29329679/standardization-of-a-fast-and-effective-method-for-the-generation-and-detection-of-platelet-derived-microparticles-by-a-flow-cytometer
#2
Daniela Recabarren-Leiva, Marcelo Alarcón
The Flow Cytometry is the principal method used to measure platelet-derived microparticles (PDMPs), by fluorescent properties analysis. PDMPs (0.1-1.0 microns) are abundant in circulation, accounting for approximately 90% of the microparticles and are associated with Cardiovascular Disease, the leading cause of death in the world.
January 9, 2018: Immunology Letters
https://www.readbyqxmd.com/read/29305843/circulating-microparticles-levels-are-increased-in-patients-with-diabetic-kidney-disease-a-case-control-research
#3
Kathryna Fontana Rodrigues, Nathalia Teixeira Pietrani, Ana Paula Fernandes, Adriana Aparecida Bosco, Maira Cândida Rodrigues de Sousa, Ieda de Fátima Oliveira Silva, Josianne Nicácio Silveira, Fernanda Magalhães Freire Campos, Karina Braga Gomes
BACKGROUND: Type 2 diabetes mellitus (T2DM) is associated with chronic lowgrade inflammation. Microparticles (MPs) are extracellular microvesicles released during apoptosis and cellular activation. The MP's pro-coagulant and pro-inflammatory activities are involved in endothelial dysfunction observed in T2DM patients. This study aimed to evaluate the circulating MPs profile in T2DM patients with diabetic kidney disease (DKD) and correlate it with clinical and laboratorial parameters. METHODS: MPs derived from platelets (PMPs), leukocytes (LMPs), endothelial cells (EMPs), and expressing tissue factor (TFMPs) were measured by flow cytometry, in plasma of 39 DKD patients and 30 non-diabetic controls...
January 3, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29228624/platelet-microparticle-mediated-transfer-of-mir-939-to-epithelial-ovarian-cancer-cells-promotes-epithelial-to-mesenchymal-transition
#4
Meiling Tang, Lu Jiang, Yingying Lin, Xiaoli Wu, Kai Wang, Qizhi He, Xipeng Wang, Weiping Li
Epithelial ovarian cancer (EOC) patients frequently suffer from thrombocytosis, which leads to a poor prognosis. However, the mechanism underlying platelet regulation of biological behavior in EOC remains unclear. The associations between clinicopathological characteristics and thrombocytosis in 171 EOC patients were studied, preoperative thrombocytosis was significantly associated with the stage, metastasis scope, level of preoperative CA125 and overall survival. When SKOV3 cells were cocultured with platelet microparticles (PMPs), the expression of molecules associated with epithelial-mesenchymal transition (EMT) was increased...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228619/fibrin-bearing-microparticles-marker-of-thrombo-embolic-events-in-pancreatic-and-colorectal-cancers
#5
Diane Mege, Lydie Crescence, Mehdi Ouaissi, Igor Sielezneff, Regis Guieu, Françoise Dignat-George, Christophe Dubois, Laurence Panicot-Dubois
Background: Microparticles (MPs) are plasma membrane-derived extracellular vesicles present in the bloodstream. We have described a specific signature of MPs, called microparticulosome, in colorectal (CRC) and pancreatic (PC) cancers. We observed that levels of fibrin-bearing MPs were significantly increased in patients suffering from PC and CRC in comparison with control groups. Here, we hypothesised that fibrin-MPs may constitute a relevant biomarker of thrombosis associated with cancer...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211120/cell-derived-microparticles-in-gingival-crevicular-fluid-from-periodontitis-patients-with-type-2-diabetes
#6
Carlos Marcelo Figueredo, Ronaldo Lira, Manuela Rubim Sete, Ricardo Guimarães Fischer
Cell-derived microparticles (MPs) have been described as vital contributors to the inflammatory process. However, its role in the periodontal disease pathogenesis remains unclear. Therefore, we aimed to detect the presence neutrophil (CD66b+) and platelet (CD41b+) derived microparticles in gingival crevicular fluid from individuals having periodontitis aggravated by type 2 diabetes. Twelve patients (56.2 ±7.2 yrs) with severe form of chronic periodontitis aggravated by type 2 diabetes were included. Clinical and metabolic data were gathered...
November 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29209923/plasma-microparticles-in-alzheimer-s-disease-the-role-of-vascular-dysfunction
#7
Soheila Hosseinzadeh, Maryam Noroozian, Esmaeil Mortaz, Kazem Mousavizadeh
Cerebrovascular lesions, a potent stimulus for endothelial cell activation, trigger cognitive and degenerative changes and contribute to pathology of Alzheimer's disease (AD). Circulating microparticles (MPs) are actively involved in the pathogenesis of AD and cerebrovascular diseases, which share common vascular risk factors. We examined the plasma changes of endothelial MPs (EMPs) and platelet MPs (PMPs) in AD patients with vascular risk factors. The plasma Annexin V+ CD 41a- CD144+ EMPs and Annexin V+ CD41a+ CD144- PMPs of 37 patients with AD, with or without vascular risk factors (hypertension, diabetes, dyslipidemia, stroke, coronary artery disease, and smoking), and 10 age-matched controls were quantified by flow cytometry...
December 5, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29183152/marathon-running-increases-circulating-endothelial-and-thrombocyte-derived-microparticles
#8
Viktoria Schwarz, Philip Düsing, Thomas Liman, Christian Werner, Juliane Herm, Katrin Bachelier, Matthias Krüll, Lars Brechtel, Gerhard J Jungehulsing, Wilhelm Haverkamp, Michael Böhm, Matthias Endres, Karl Georg Haeusler, Ulrich Laufs
Background Acute vascular effects of high intensity physical activity are incompletely characterized. Circulating microparticles are cellular markers for vascular activation and damage. Methods Microparticles were analysed in 99 marathon runners (49 ± 6 years, 22% female) of the prospective Berlin Beat of Running study. Blood samples were taken within three days before, immediately after and within two days after the marathon run. Endothelial-derived microparticles were labelled with CD144, CD31 and CD62E, platelet-derived microparticles with CD62P and CD42b, leukocyte-derived microparticles with CD45 and monocyte-derived microparticles with CD14...
January 1, 2017: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/29180664/circulating-microparticles-in-acute-diabetic-charcot-foot-exhibit-a-high-content-of-inflammatory-cytokines-and-support-monocyte-to-osteoclast-cell-induction
#9
Jennifer Pasquier, Binitha Thomas, Jessica Hoarau-Véchot, Tala Odeh, Amal Robay, Omar Chidiac, Soha R Dargham, Rebal Turjoman, Anna Halama, Khalid Fakhro, Robert Menzies, Amin Jayyousi, Mahmoud Zirie, Jassim Al Suwaidi, Arash Rafii, Rayaz A Malik, Talal Talal, Charbel Abi Khalil
Circulating microparticles (MPs) are major mediators in cardiovascular complications of type 2 diabetes (T2D); however, their contribution to Charcot foot (CF) disease is not known. Here, we purified and assessed the origin, concentration and content of circulating MPs from 33 individuals: 11 with T2D and acute CF, 11 T2D patients with equivalent neuropathy and 11 non-diabetic controls. First, we demonstrated that there were no differences in the distribution of MPs of endothelial, platelet origin among the 3 groups...
November 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29130961/endothelial-cell-derived-microparticles-from-patients-with-obstructive-sleep-apnea-hypoxia-syndrome-and-coronary-artery-disease-increase-aortic-endothelial-cell-dysfunction
#10
Lixin Jia, Jingyao Fan, Wei Cui, Sa Liu, Na Li, Wayne Bond Lau, Xinliang Ma, Jie Du, Shaoping Nie, Yongxiang Wei
BACKGROUND/AIMS: Obstructive sleep apnea hypoxia syndrome (OSAHS) is an independent risk factor for coronary artery disease (CAD). Treatment of OSAHS improves clinical outcome in some CAD patients, but the relationship between OSAHS and CAD is complex. Microparticles (MPs) are shed by the plasma membrane by either physiologic or pathologic stimulation. In the current study, we investigated the role of MPs in the context of OSAHS. METHODS AND RESULTS: 54 patients with both suspected coronary artery stenosis and OSAHS were recruited and underwent both coronary arteriography and polysomnography...
November 1, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29126756/-vascular-dysfunction-in-obstructive-sleep-apnoea-implication-of-microparticules
#11
REVIEW
W Trzepizur, P Priou, N Meslier, T Urban, M C Martínez, R Andriantsitohaina, F Gagnadoux
Obstructive sleep apnea (OSA) is associated with increased cardiovascular diseases, including myocardial infarction and stroke and promotes cardiovascular risk factors including diabetes and hypertension. OSA has also been proposed to have a direct proatherogenic effects. Recent studies have investigated the role of microparticles (MPs) in the atherogenic process. MPs are small plasma membrane vesicles that can be released by a variety of vascular or blood cells and that contain membrane and cytosolic elements...
November 7, 2017: Revue de Pneumologie Clinique
https://www.readbyqxmd.com/read/29101610/endothelial-microparticles-are-associated-to-pathogenesis-of-idiopathic-pulmonary-fibrosis
#12
Nour C Bacha, Adeline Blandinieres, Elisa Rossi, Nicolas Gendron, Nathalie Nevo, Séverine Lecourt, Coralie L Guerin, Jean Marie Renard, Pascale Gaussem, Eduardo Angles-Cano, Chantal M Boulanger, Dominique Israel-Biet, David M Smadja
Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Microparticles (MPs) have been investigated in several human lung diseases as possible pathogenic elements, prognosis markers and therapeutic targets. We postulated that levels and cellular origins of circulating MPs might serve as biomarkers in IPF patients and/or as active players of fibrogenesis...
November 3, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29039531/prognostic-implications-and-procoagulant-activity-of-phosphatidylserine-exposure-of-blood-cells-and-microparticles-in-patients-with-atrial-fibrillation-treated-with-pulmonary-vein-isolation
#13
Huan Meng, Junjie Kou, Ruishuang Ma, Wenbo Ding, Yan Kou, Muhua Cao, Zengxiang Dong, Yayan Bi, Hemant S Thatte, Jialan Shi
The present study aimed to evaluate the procoagulant effects of phosphatidylserine (PS) exposure on blood cells and microparticles (MPs), and examine its role in predicting early recurrence atrial fibrillation (ERAF) in patients with atrial fibrillation (AF) treated with pulmonary vein isolation (PVI). Blood samples were obtained from 40 healthy controls and 56 patients with AF at baseline (prior to PVI), and 0, 1 h, 1 day, 3 days and 7 days following PVI. The exposure of PS (PS+) to blood cells (platelets, erythrocytes and leukocytes) and MPs was detected using flow cytometry...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28980454/incidence-risk-factors-and-outcomes-of-central-venous-catheter-related-thromboembolism-in-breast-cancer-patients-the-caveccas-study
#14
Philippe Debourdeau, Marc Espié, Sylvie Chevret, Joseph Gligorov, Antoine Elias, Pierre François Dupré, Kristell Desseaux, Issa Kalidi, Stephane Villiers, Sylvie Giachetti, Corinne Frere, Dominique Farge
Previous epidemiologic studies investigating central venous catheter (CVC)-related venous thromboembolism (CRT) were conducted in heterogenous cancer populations and data in breast cancer (BC) remain limited. To investigate the Doppler ultrasound (DUS)-CRT incidence, risk factors and outcomes in BC, we designed a prospective, multicenter cohort of nonmetastatic invasive BC patients undergoing insertion of a CVC for chemotherapy. All patients underwent double-blind DUS before, 7, 30, and 90 days after CVC insertion and a 6 months clinical follow-up...
November 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28972406/brain-derived-microparticles-in-patients-with-severe-isolated-tbi
#15
M Nekludov, B-M Bellander, D Gryth, H Wallen, F Mobarrez
PRIMARY OBJECTIVE: to investigate the presence of circulating microparticles (MPs) of brain tissue origin in the systemic and cerebrovenous blood of patients with severe traumatic brain injury (TBI). RESEARCH DESIGN: Prospective observational study in 15 consecutive patients with severe isolated TBI. METHODS AND PROCEDURES: We repeatedly measured concentrations of MPs expressing glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) and aquaporin-4 (AQP4), in arterial and cerebrovenous blood at admittance to hospital and up to 72 hours after the injury...
2017: Brain Injury: [BI]
https://www.readbyqxmd.com/read/28906481/insights-into-the-diagnostic-potential-of-extracellular-vesicles-and-their-mirna-signature-from-liquid-biopsy-as-early-biomarkers-of-diabetic-micro-macrovascular-complications
#16
REVIEW
Valeria La Marca, Alessandra Fierabracci
Extracellular vesicles (EVs) represent a heterogeneous population of small vesicles, consisting of a phospholipidic bilayer surrounding a soluble interior cargo. Almost all cell types release EVs, thus they are naturally present in all body fluids. Among the several potential applications, EVs could be used as drug delivery vehicles in disease treatment, in immune therapy because of their immunomodulatory properties and in regenerative medicine. In addition to general markers, EVs are characterized by the presence of specific biomarkers (proteins and miRNAs) that allow the identification of their cell or tissue origin...
September 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28883845/the-impact-of-hyperglycemia-and-hyperlipidemia-on-plasma-p-selectin-and-platelet-markers-after-ischemic-stroke
#17
Malgorzata Pawelczyk, Beata Kaczorowska, Zbigniew Baj
INTRODUCTION: Platelet activation plays a key role in the pathogenesis of ischemic cerebrovascular diseases. Thus, it is very important to identify novel pharmacological targets for platelet inhibition to improve ischemic stroke treatment. The aim of the study was to assess the relationship between metabolic disorders and platelet activity markers in patients with acute ischemic stroke. MATERIAL AND METHODS: Ninety-four patients with acute ischemic stroke were divided into four groups with: normolipidemia and normoglycemia (NL/NG), n = 25; normolipidemia and hyperglycemia (NL/HG), n = 21; hyperlipidemia and normoglycemia (HL/NG), n = 27; hyperlipidemia and hyperglycemia (NL/NG), n = 21...
August 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28882977/influence-of-diabetes-on-circulating-apoptotic-microparticles-in-patients-with-chronic-hepatitis-c
#18
Jolanta Zuwala-Jagiello, Monika Pazgan-Simon, Eugenia Murawska-Cialowicz, Krzysztof Simon
BACKGROUND/AIM: Type 2 diabetes mellitus (DM) frequently occurs in patients with chronic hepatitis C (CHC) and is associated with atherosclerosis, in which circulating microparticles (MPs) play an important role. We asked whether the presence of DM affects endothelial-derived (EMPs) and platelet-derived microparticles (PMPs) levels; and whether MPs levels associate with biomarkers of inflammation and oxidative stress in patients with CHC. MATERIALS AND METHODS: Overall, 136 patients were enrolled in the study, 86 CHC patients (41 with DM with moderate glycemic control), 20 outpatients with DM and 30 controls...
September 2017: In Vivo
https://www.readbyqxmd.com/read/28841240/preanalytic-processing-of-rat-plasma-influences-thrombin-generation-and-fibrinolysis-assays
#19
Marjory B Brooks, Alyssa P Stablein, Lynn Johnson, Albert Eric Schultze
BACKGROUND: Thrombin generation assays (TGA) have potential applications as measures of hemostatic balance in animal models. However, variations in plasma processing greatly influence human TGA, and may also impact on the translational value of TGA in animal studies. OBJECTIVES: The purpose of the study was to compare the performance characteristics of Sprague-Dawley rat plasma prepared by single vs double centrifugation protocols in TGA and fibrinolysis assays...
August 25, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28836262/immune-status-of-patients-with-haemophilia-a-before-exposure-to-factor-viii-first-results-from-the-hemfil-study
#20
MULTICENTER STUDY
Letícia L Jardim, Daniel G Chaves, Amanda C O Silveira-Cassette, Ana Cristina Simões E Silva, Marcio P Santana, Monica H Cerqueira, Alessandra Prezotti, Claudia Lorenzato, Vivian Franco, Johanna G van der Bom, Suely M Rezende
Previous cross-sectional studies showed that some patients with haemophilia A (HA) without inhibitor presented a pro-inflammatory profile during factor VIII (FVIII) replacement therapy. Furthermore, an anti-inflammatory/regulatory state was described in HA patients after inhibitor development. However, no study investigated the levels of these biomarkers before exposure to exogenous FVIII. This study investigated the immunological profile of previously untreated patients (PUPs) with HA in comparison with non-haemophiliac boys...
September 2017: British Journal of Haematology
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