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Platelet derived microparticle

Robert Kölm, Monica Schaller, Lubka T Roumenina, Iga Niemiec, Johanna A Kremer Hovinga, Elham Khanicheh, Beat A Kaufmann, Helmut Hopfer, Marten Trendelenburg
Premature atherosclerosis and thrombotic complications are major causes of morbidity and mortality in patients with systemic lupus erythematosus (SLE). However, the high incidence of these complications cannot be explained by traditional risk factors alone, suggesting direct effects of an activated immune system on hemostasis. The unexpected nucleotide sequence homology between SLE patient -: derived autoantibodies against complement C1q (Fab anti-C1q) and von Willebrand factor (VWF) led us to investigate a potential interaction between the complement and hemostatic systems on the level of initiating molecules...
October 3, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Alice Giacomazzi, Maurizio Degan, Stefano Calabria, Alessandra Meneguzzi, Pietro Minuz
Platelet microparticles (PMPs) contribute to thrombogenesis but the effects of antiplatelet drugs on PMPs generation is undefined. The present study investigated the cellular events regulating PMPs shedding, testing in vitro platelet agonists and inhibitors. Platelet-rich plasma from healthy subjects was stimulated with arachidonic acid (AA), U46619, collagen type-I (10 and 1.5 μg/mL), epinephrine, ADP or TRAP-6 and pre-incubated with acetylsalicylic acid (ASA, 100 and 10 μmol/L), SQ-29,548, apyrase, PSB-0739, or eptifibatide...
2016: Frontiers in Pharmacology
Jun-Yi Zhu, Jian-Gang Ren, Wei Zhang, Feng-Qin Wang, Yu Cai, Ji-Hong Zhao, Gang Chen, Yi-Fang Zhao
OBJECTIVES: To determine the basic biochemical features of microparticles (MP) in patients with venous malformation (VM) of the head and neck. METHODS: MP were isolated from peripheral venous blood of VM patients or healthy subjects and from lesional fluid of VM patients. Flow cytometry and real-time polymerase chain reaction were employed to determine the concentration, cellular origin and RNA expression of obtained MP. A functional coagulation test was applied to measure the coagulant activity of MP...
September 26, 2016: Oral Diseases
S Cointe, C Judicone, S Robert, M J Mooberry, P Poncelet, M Wauben, R Nieuwland, N S Key, F Dignat-George, R Lacroix
BACKGROUND: Microparticles are extracellular vesicles resulting from the budding of cellular membranes that have a high potential as emergent biomarkers; however, their clinical relevance is hampered by methodological enumeration concerns and a lack of standardization. Flow cytometry (FCM) remains the most commonly used technique with the best capability to determine the cellular origin of single MPs. However, instruments behave variably depending on which scatter parameter, (Forward (FSC) or Side scatter (SSC)), provides the best resolution to discriminate submicron particles...
September 23, 2016: Journal of Thrombosis and Haemostasis: JTH
Pedro S Babo, Pedro P Carvalho, Vítor E Santo, Susana Faria, Manuela E Gomes, Rui L Reis
Injectable calcium phosphate cements have been used as a valid alternative to autologous bone grafts for bone augmentation with the additional advantage of enabling minimally invasive implantation procedures and for perfectly fitting the tissue defect. Nevertheless, they have low biodegradability and lack adequate biochemical signaling to promote bone healing and remodeling. In previous in vitro studies, we observed that the incorporation of platelet lysate directly into the cement paste or loaded in hyaluronic acid microspheres allowed to modulate the cement degradation and the in vitro expression of osteogenic markers in seeded human adipose derived stem cells...
September 16, 2016: Journal of Biomaterials Applications
V Schwarz, K Bachelier, S H Schirmer, C Werner, U Laufs, M Böhm
BACKGROUND: Moderate consumption of red wine is associated with less cardiovascular events. We investigated whether red wine consumption counteracts the adverse vascular effects of cigarette smoking. METHODS: Participants smoked three cigarettes, alone or after drinking a titrated volume of red wine. Clinical chemistry, blood counts, plasma cytokine ELISAs, immuno-magnetic separation of CD14(+) monocytes for gene expression analysis, fluorescence-activated cell sorting for microparticles, isolation of circulating mononuclear cells to measure telomerase activity were performed and urine cotinine levels were quantified...
September 12, 2016: American Journal of Medicine
Adam J Mitchell, Warren D Gray, Salim S Hayek, Yi-An Ko, Sheena Thomas, Kim Rooney, Mosaab Awad, John D Roback, Arshed Quyyumi, Charles D Searles
Extracellular miRNAs are detectable in biofluids and represent a novel class of disease biomarker. Although many studies have utilized archived plasma for miRNA biomarker discovery, the effects of processing and storage have not been rigorously studied. Previous reports have suggested plasma samples are commonly contaminated by platelets, significantly confounding the measurement of extracellular miRNA, which was thought to be easily addressed by additional post-thaw plasma processing. In a case-control study of archived plasma, we noted a significant correlation between miRNA levels and platelet counts despite post-thaw processing...
2016: Scientific Reports
Sameh Obeid, Adam Ceroi, Guillaume Mourey, Philippe Saas, Celine Elie-Caille, Wilfrid Boireau
Blood microparticles (MPs) are small membrane vesicles (50-1000nm), derived from different cell types. They are known to play important roles in various biological processes and also recognized as potential biomarkers of various health disorders. Different methods are currently used for the detection and characterization of MPs, but none of these methods is capable to quantify and qualify total MPs at the same time, hence, there is a need to develop a new approach for simultaneous detection, characterization and quantification of microparticles...
August 29, 2016: Biosensors & Bioelectronics
Andrew L Frelinger, Anja J Gerrits, Allen L Garner, Andrew S Torres, Antonio Caiafa, Christine A Morton, Michelle A Berny-Lang, Sabrina L Carmichael, V Bogdan Neculaes, Alan D Michelson
BACKGROUND: Activated autologous platelet-rich plasma (PRP) used in therapeutic wound healing applications is poorly characterized and standardized. Using pulsed electric fields (PEF) to activate platelets may reduce variability and eliminate complications associated with the use of bovine thrombin. We previously reported that exposing PRP to sub-microsecond duration, high electric field (SMHEF) pulses generates a greater number of platelet-derived microparticles, increased expression of prothrombotic platelet surfaces, and differential release of growth factors compared to thrombin...
2016: PloS One
Lixiu Wang, Yayan Bi, Muhua Cao, Ruishuang Ma, Xiaoming Wu, Yan Zhang, Wenbo Ding, Yan Liu, Qian Yu, Yingqian Zhang, Hua Jiang, Yingchun Sun, Dongxia Tong, Li Guo, Zengxiang Dong, Ye Tian, Junjie Kou, Jialan Shi
BACKGROUND: Relatively little is known about the role of phosphatidylserine (PS) in procoagulant activity (PCA) in patients with non-ST-elevated myocardial infarction (NSTEMI) after stent implantation. This study was designed to evaluate whether exposed PS on microparticles (MPs) and blood cells were involved in the hypercoagulable state in NSTEMI patients with stent implantation. METHODS: NSTEMI patients (n=90) and healthy controls (n=20) were included in our study...
November 15, 2016: International Journal of Cardiology
Qianghua Hu, Min Soon Cho, Perumal Thiagarajan, Fleur M Aung, Anil K Sood, Vahid Afshar-Kharghan
Cyclooxygenase (COX) is the rate-limiting enzyme in conversion of arachidonic acid to prostanoids, and has two isoforms, COX1 and COX2, which share ~65% amino acid homology. COX1 is universally expressed in many cell types including platelets; however, expression of COX2 is known to be more limited. We examined expression of COX2 mRNA and protein in platelets and platelet-derived microparticles (MPs); using quantitative RT-PCR, immunostaining, and Western blotting. We have detected a significant amount of COX2 in platelets, both at mRNA and protein levels...
August 18, 2016: Platelets
Daryl Crimmins, Peter Flanagan, Richard Charlewood, Katya Ruggiero
BACKGROUND: Cryopreserved platelet (PLT) components stored at -80°C in 5% to 6% dimethyl sulfoxide (DMSO) demonstrate enhanced hemostatic activity. Alterations in PLT surface glycoprotein expression and release of procoagulant microparticles during the freeze/thaw cycle result in PLT activation. Nothing is known of the effect of gamma irradiation on the in vitro quality of reconstituted cryopreserved PLTs. STUDY DESIGN AND METHODS: Gamma-irradiated (25-50 Gy) buffy coat-derived PLT components were either stored at room temperature for 7 days (the current expiry in New Zealand) or cryopreserved at -80°C using 5% to 6% DMSO...
August 16, 2016: Transfusion
Patrizia Amadio, Damiano Baldassarre, Leonardo Sandrini, Babette B Weksler, Elena Tremoli, Silvia S Barbieri
Cigarette smoke (CS) activates platelets, promotes vascular dysfunction, and enhances Tissue Factor (TF) expression in blood monocytes favoring pro-thrombotic states. Brain-derived neurotrophic factor (BDNF), a member of the family of neurotrophins involved in survival, growth, and maturation of neurons, is released by activated platelets (APLTs) and plays a role in the cardiovascular system. The effect of CS on circulating levels of BDNF is controversial and the function of circulating BDNF in atherothrombosis is not fully understood...
August 5, 2016: Platelets
Anna Sina P Meyer, Sisse R Ostrowski, Jesper Kjaergaard, Pär I Johansson, Christian Hassager
BACKGROUND: Morbidity and mortality following initial survival of cardiac arrest remain high despite great efforts to improve resuscitation techniques and post-resuscitation care, in part due to the ischemia-reperfusion injury secondary to the restoration of the blood circulation. Patients resuscitated from cardiac arrest display evidence of endothelial injury and coagulopathy (hypocoagulability, hyperfibrinolysis), which in associated with poor outcome. Recent randomized controlled trials have revealed that treatment with infusion of prostacyclin reduces endothelial damage after major surgery and AMI...
2016: Trials
Qianghua Hu, Miao Wang, Min Soon Cho, Chunyan Wang, Alpa M Nick, Perumal Thiagarajan, Fleur M Aung, Xianlin Han, Anil K Sood, Vahid Afshar-Kharghan
BACKGROUND: Ovarian cancer patients have a high risk of developing venous thrombosis. The membrane lipid bilayer of platelets and platelet-derived microparticles (PMP) provides a platform for assembly of coagulation proteins and generation of blood clots. METHODS: We compared the lipid composition of platelets and PMPs in patients with ovarian cancer to those in healthy subjects. We used shotgun lipidomics to quantify 12 classes and 177 species of lipids. RESULTS: We found a significant change in 2 classes of lipids in platelets and PMPs isolated from ovarian cancer patients: higher phosphatidylinositol and lower lyso-phosphatidylcholine...
December 2016: BBA Clinical
Nina Olausson, Fariborz Mobarrez, Håkan Wallen, Eli Westerlund, Outi Hovatta, Peter Henriksson
Cell-derived microparticles (MPs) are known to be elevated in a number of diseases related to arterial and venous thromboembolism (VTE), such as acute myocardial infarction, VTE (deep-vein thrombosis and pulmonary embolism) and peripheral arterial disease. IVF-associated pregnancies have previously been shown to be associated with an increased incidence of VTE, mechanisms behind being unknown and sparsely studied. Our objective was to assess cell activation during IVF through analysis of MP levels and phenotype following ovarian stimulation...
August 30, 2016: Thrombosis and Haemostasis
Jan-Malte Sinning, Felix Jansen, Christoph Hammerstingl, Arne Meier, Jan Losch, Katharina Rohwer, Theresa Schmitz, Kathrin Paul, Alexander Sedaghat, Robert Schueler, Mariuca Vasa-Nicotera, Cornelius Müller, Georg Nickenig, Nikos Werner
BACKGROUND: Cardiac stress leads to a dynamic increase of circulating microparticles (MPs) in healthy individuals that is diminished in individuals with vascular disease. The impact of coronary ischemia on circulating MP level is unknown. This study investigates the kinetics of circulating MPs during cardiac stress in patients with coronary artery stenosis. HYPOTHESIS: Patients with significant coronary stenosis show altered circulating MP levels after cardiac stress...
July 13, 2016: Clinical Cardiology
Xavier Delabranche, Jean-Pierre Quenot, Thierry Lavigne, Emmanuelle Mercier, Bruno François, François Severac, Lélia Grunebaum, Madah Mehdi, Fatiha Zobairi, Florence Toti, Ferhat Meziani, Julie Boisramé-Helms
OBJECTIVES: Inadequate stratification of septic shock patients may result in inappropriate treatment allocation in randomized clinical trials, especially regarding anticoagulant. We previously reported that endothelial-derived microparticles are relevant biomarkers of sepsis-induced disseminated intravascular coagulation. In this validation cohort, we assess microparticles as surrogates of cell activation to improve early disseminated intravascular coagulation diagnosis and patient stratification...
October 2016: Critical Care Medicine
A Papapanagiotou, G Daskalakis, G Siasos, A Gargalionis, A G Papavassiliou
The role of platelets in atherosclerotic process and subsequently in the pathophysiology of cardiovascular disease is essential as platelets in addition to their contribution to thrombosis and hemostasis modulate inflammatory reactions and immune response. Platelets after adhesion on the injured vascular endothelium and activation release a wide range of molecules stored in platelets granules such as chemokines, proinflammatory molecules and other biological response modulators accelerating interaction among platelets, endothelial cells and leukocytes...
June 6, 2016: Current Pharmaceutical Design
S G Khaspekova, O A Antonova, O N Shustova, V V Yakushkin, N V Golubeva, E V Titaeva, A B Dobrovolsky, A V Mazurov
Activity of tissue factor (TF) in membrane microparticles (MPs) produced in vitro by endothelial cells (ECs), monocytes, THP-1 monocytic cells, granulocytes, and platelets was investigated. ECs were isolated from human umbilical vein, and monocytes, granulocytes, and platelets - from the blood of healthy donors. ECs, monocytes, and THP-1 cells were activated by bacterial lipopolysaccharide, granulocytes - by lipopolysaccharide or phorbol myristate acetate, and platelets - by SFLLRN, thrombin receptor-activating peptide...
February 2016: Biochemistry. Biokhimii︠a︡
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