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https://www.readbyqxmd.com/read/28543876/application-of-molecular-microbiological-and-immunological-tests-for-the-diagnosis-of-bone-and-joint-tuberculosis
#1
Yinghua Tang, Lianli Yin, Shifu Tang, Hongyu Zhang, Jihui Lan
BACKGROUND: To evaluate the application of interferon gamma release assay (IGRA), rifampicin resistant real-time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis/rifampicin (Xpert MTB/RIF), and the levels of TNF-α and TGF-β in the diagnosis of bone and joint tuberculosis. METHODS: Eighty-six patients with bone and joint tuberculosis, diagnosed by pathology or microbiology, were examined by Xpert MTB/RIF and IGRA (T-SPOT. TB) for Mycobacterium tuberculosis infection, and the TNF-α and TGF-β levels of the patients were measured...
May 22, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28542477/mycobacterium-tuberculosis-arrests-host-cycle-at-the-g1-s-transition-to-establish-long-term-infection
#2
Bridgette M Cumming, Md Aejazur Rahman, Dirk A Lamprecht, Kyle H Rohde, Vikram Saini, John H Adamson, David G Russell, Adrie J C Steyn
Signals modulating the production of Mycobacterium tuberculosis (Mtb) virulence factors essential for establishing long-term persistent infection are unknown. The WhiB3 redox regulator is known to regulate the production of Mtb virulence factors, however the mechanisms of this modulation are unknown. To advance our understanding of the mechanisms involved in WhiB3 regulation, we performed Mtb in vitro, intraphagosomal and infected host expression analyses. Our Mtb expression analyses in conjunction with extracellular flux analyses demonstrated that WhiB3 maintains bioenergetic homeostasis in response to available carbon sources found in vivo to establish Mtb infection...
May 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28531231/utility-of-th1-cell-immune-responses-for-distinguishing-active-tuberculosis-from-non-active-tuberculosis-a-case-control-study
#3
Lifan Zhang, Xinhe Cheng, Sainan Bian, Yanhua Song, Qiang Li, Mengqiu Gao, Yueqiu Zhang, Xiaochun Shi, Xiaoqing Liu
Currently available Interferon-γ release assay (IGRA) cannot reliably differentiate active TB (ATB) from non-active TB (non-ATB). A study was performed to evaluate the value of Mycobacterium tuberculosis (MTB) specific Th1 cell immune responses which test IFN-γ and IL-2 simultaneous for differentiating ATB from non-ATB. Forty-nine newly diagnosed inpatients with ATB (26 pulmonary TB and 23 extrapulmonary TB) were enrolled as the ATB group. Forty-five volunteers with latent tuberculosis infection (LTBI) and twenty with evidence of previous TB were enrolled during the same period as the non-ATB group...
2017: PloS One
https://www.readbyqxmd.com/read/28529202/an-evaluation-of-automated-chest-radiography-reading-software-for-tuberculosis-screening-among-public-and-private-sector-patients
#4
Md Toufiq Rahman, Andrew J Codlin, Md Mahfuzur Rahman, Ayenun Nahar, Mehdi Reja, Tariqul Islam, Zhi Zhen Qin, Md Abdus Shakur Khan, Sayera Banu, Jacob Creswell
Computer-aided reading (CAR) of medical images is becoming increasingly common, but few studies exist for CAR in tuberculosis (TB). We designed a prospective study evaluating CAR for chest radiography (CXR) as a triage tool before Xpert MTB/RIF (Xpert).Consecutively enrolled adults in Dhaka, Bangladesh, with TB symptoms received CXR and Xpert. Each image was scored by CAR and graded by a radiologist. We compared CAR with the radiologist for sensitivity and specificity, area under the receiver operating characteristic curve (AUC), and calculated the potential Xpert tests saved...
May 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28522948/evaluation-of-xpert-mtb-rif-assay-performance-in-the-diagnosis-of-abdominal-tuberculosis
#5
Suraj Kumar, Sawan Bopanna, Saurabh Kedia, Pratap Mouli, Rajan Dhingra, Rajesh Padhan, Mikashmi Kohli, Jigyasa Chaubey, Rohini Sharma, Prasenjit Das, S Dattagupta, Govind Makharia, S K Sharma, Vineet Ahuja
BACKGROUND/AIMS: The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utility of the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant (MDR) TB in the Indian population. METHODS: Of 99 patients recruited, 37 had intestinal TB; two control groups comprised 43 with Crohn's disease (CD) and 19 with irritable bowel syndrome...
April 2017: Intestinal Research
https://www.readbyqxmd.com/read/28521188/bis-diphenylphosphino-amines-containing-ruthenium-cymene-complexes-as-potential-anti-mycobacterium-tuberculosis-agents
#6
Juliana P da Silva, Isabel C Silva, Fernando R Pavan, Davi F Back, Márcio P de Araujo
Several ruthenium complexes have been investigated regarding anti-Mycobacterium tuberculosis (anti-MTb) activity, with some diphosphine-containing ruthenium complexes comparable to first and second line drugs. However, to the best of our knowledge, there is no PNP-containing ruthenium complexes applied as metallodrugs. Thus, this study focused on the synthesis, characterization and anti-MTb activity of a new series of coordination compounds with general formula [RuCl(η(6)-p-cymene)(PN(R)P)]X (R=CH2Py (Py=pyridine)-[1a], CH2Ph (Ph=phenyl)-[1b], Ph-[1c] and p-tol (p-tol=p-tolyl)-[1d]; X=PF6(-) or BF4(-))...
April 9, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28518163/exploitation-by-mtb
#7
Laurie A Dempsey
No abstract text is available yet for this article.
May 18, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28516818/the-changing-epidemiology-of-spinal-tuberculosis-the-influence-of-international-immigration-in-catalonia-1993-2014
#8
M Peghin, D Rodriguez-Pardo, A Sanchez-Montalva, F Pellisé, A Rivas, T Tortola, J Aguilar, B Almirante, C Pigrau
The overall incidence of spinal tuberculosis (TB) appears to be stable or declining in most European countries, but with an increasing proportion of cases in the foreign-born populations. We performed a retrospective observational study (1993-2014), including all cases of spinal TB diagnosed at a Barcelona hospital to assess the epidemiological changes. Fifty-four episodes (48·1% males, median age 52 years) of spinal TB were diagnosed. The percentage of foreign-born residents with spinal TB increased from 14% to 45·2% in the last 10 years (P = 0·017)...
May 18, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28515134/identification-of-novel-seroreactive-antigens-in-johne-s-disease-cattle-using-the-mycobacterium-tuberculosis-protein-array
#9
John P Bannantine, Joseph J Campo, Lingling Li, Arlo Randall, Jozelyn Pablo, Craig A Praul, Juan Antonio Raygoza Garay, Judith R Stabel, Vivek Kapur
Johne's Disease, a chronic gastrointestinal inflammatory disease caused by Mycobacterium avium subspecies paratuberculosis (Map), is endemic in dairy cattle and other ruminants worldwide and remains a challenge to diagnose using traditional serological methods. Given the close phylogenetic relationship between Map and the human pathogen, Mycobacterium tuberculosis (Mtb), we here applied a whole proteome Mtb protein array to identify seroreactive and diagnostic Map antigens. A genome-scale pairwise analysis of amino acid identity between orthologous proteins in Map and Mtb showed an average of 62% identity, with more than half the orthologous proteins sharing > 75% identity...
May 17, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28513917/fragment-screening-against-the-ethr-dna-interaction-by-native-mass-spectrometry
#10
Daniel Shiu-Hin Chan, Vitor Mendes, Sherine E Thomas, Brendan N McConnell, Dijana Matak-Vinković, Anthony G Coyne, Tom L Blundell, Christopher Abell
Native nanoelectrospray ionization mass spectrometry is an underutilized technique for fragment screening. In this study, the first demonstration is provided of the use of native mass spectrometry for screening fragments against a protein-DNA interaction. EthR is a transcriptional repressor of EthA expression in Mycobacterium tuberculosis (Mtb) that reduces the efficacy of ethionamide, a second-line anti-tubercular drug used to combat multidrug resistant Mtb strains. A small-scale fragment screening campaign was conducted against the EthR-DNA interaction using native mass spectrometry, and results were compared with those from differential scanning fluorimetry, a commonly used primary screening technique...
May 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28511895/diagnostic-performance-of-anyplex-ii-mtb-mdr-xdr-for-detection-of-resistance-to-first-and-second-line-drugs-in-mycobacterium-tuberculosis
#11
F Pérez-García, M J Ruiz-Serrano, P López Roa, Fermín Acosta, Laura Pérez-Lago, D García-De-Viedma, E Bouza
PURPOSE: Genotypic methods have considerably improved the diagnosis of multidrug-resistant (MDR) tuberculosis. One of these tests is Anyplex II MTB/MDR/XDR (Anyplex). Our aim was to evaluate the diagnostic performance of this multiplex PCR. METHODS: We conducted our study on 47 MDR tuberculosis and 14 pan-susceptible strains. We evaluated the ability of Anyplex to detect resistance mutations in rpoB (rifampin [RIF]), katG and inhA (isoniazid [INH]), gyrA (fluoroquinolones [FLQ]), and rrs and eis (aminoglycosides [AMG])...
May 13, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28510418/conjugation-reaction-with-8-arm-peg-markedly-improves-the-immunogenicity-of-mycobacterium-tuberculosis-cfp10-tb10-4-fusion-protein
#12
Xiaowei Sun, Weili Yu, Quanhai Pang, Tao Hu
Mycobacterium tuberculosis (Mtb) is a serious fatal pathogen responsible for tuberculosis (TB). Effective vaccination is highly desired for immunoprotection against Mtb infection. CFP10 and TB10.4 are two important immunodominant Mtb-secreted protein antigens, which suffer from poor immunogenicity. Thus, an antigen delivery system and adjuvants are needed to improve the immunogenicity of the two proteins. CFP10 and TB10.4 are two Mtb-secreted immunodominant protein antigens. A CFP10-TB10.4 fusion protein (CT) was used as the antigen...
May 16, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28509874/evaluation-of-the-mycobactericidal-effect-of-thio-functionalized-carbohydrate-derivatives
#13
Małgorzata Korycka-Machała, Anna Brzostek, Bożena Dziadek, Malwina Kawka, Tomasz Popławski, Zbigniew J Witczak, Jarosław Dziadek
Sugars with heteroatoms other than oxygen have attained considerable importance in glycobiology and in drug design since they are often more stable in blood plasma due to their resistance to enzymes, such as glycosidases, phosphorylases and glycosyltransferases. The replacement of oxygen atoms in sugars with sulfur forms thio-sugars, which are potentially useful for the treatment of diabetes and some bacterial and viral infections. Here, we evaluated the antibacterial activity of thio-functionalized carbohydrate derivatives...
May 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28506804/chloroquine-enhances-the-antimycobacterial-activity-of-isoniazid-and-pyrazinamide-by-reversing-inflammation-induced-macrophage-efflux
#14
U Matt, P Selchow, M Dal Molin, S Strommer, O Sharif, K Schilcher, F Andreoni, A Stenzinger, A S Zinkernagel, M Zeitlinger, P Sander, J Nemeth
Mycobacterium tuberculosis (MTB) is notorious for persisting within host macrophages. Efflux pumps decrease intracellular drug levels, thus fostering persistence of MTB during therapy. Isoniazid (INH) and pyrazinamide (PZA) are substrates of the efflux pump breast cancer resistance protein-1 (BCRP-1), which is inhibited by chloroquine (CQ). In this study, BCRP-1 was found to be expressed on macrophages of human origin and on foamy giant cells at the site of MTB infection. In the current in vitro study, interferon-gamma (IFNγ) increased the expression of BCRP-1 in macrophages derived from the human monocytic leukaemia cell line THP-1...
May 12, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28506659/comparison-of-density-and-output-of-sweat-gland-in-tropical-africans-and-temperate-koreans
#15
JeongBeom Lee, YoungOh Shin
Modification of sweating could be due to changes in activated sweat gland density (ASGD) and/or activated sweat gland output (ASGO). The present study determined regional and inter-ethnic differences in ASGD and ASGO during passive heating between tropical natives (African, n=22) and temperate natives (Republic of Korean, n=25). Heat load was carried out by immersing the half body into a hot water bath for 30min. Tympanic temperature (Tty) and skin temperature (Ts) were measured. Mean body temperature (mTb) was calculated...
May 8, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28506215/comparison-of-antibody-responses-against-mycobacterium-tuberculosis-antigen-rv0679c-in-tuberculosis-patients-from-the-endemic-and-non-endemic-regions-of-the-beijing-genotype-a-case-control-study
#16
Jingge Zhao, Takashi Matsuba, Xiaoyan Zhang, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Elizabeth Freda Telan, Yasuhiko Suzuki, Toshio Hattori
BACKGROUND: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses...
May 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28505176/systematic-multiparametric-analysis-of-mycobacterium-tuberculosis-intracellular-infection-offers-insight-into-coordinated-virulence
#17
Amy K Barczak, Roi Avraham, Shantanu Singh, Samantha S Luo, Wei Ran Zhang, Mark-Anthony Bray, Amelia E Hinman, Matthew Thompson, Raymond M Nietupski, Aaron Golas, Paul Montgomery, Michael Fitzgerald, Roger S Smith, Dylan W White, Anna D Tischler, Anne E Carpenter, Deborah T Hung
A key to the pathogenic success of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is the capacity to survive within host macrophages. Although several factors required for this survival have been identified, a comprehensive knowledge of such factors and how they work together to manipulate the host environment to benefit bacterial survival are not well understood. To systematically identify Mtb factors required for intracellular growth, we screened an arrayed, non-redundant Mtb transposon mutant library by high-content imaging to characterize the mutant-macrophage interaction...
May 15, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28503769/identification-of-factors-required-for-m-6-a-mrna-methylation-in-arabidopsis-reveals-a-role-for-the-conserved-e3-ubiquitin-ligase-hakai
#18
Kamil Růžička, Mi Zhang, Ana Campilho, Zsuzsanna Bodi, Muhammad Kashif, Mária Saleh, Dominique Eeckhout, Sedeer El-Showk, Hongying Li, Silin Zhong, Geert De Jaeger, Nigel P Mongan, Jan Hejátko, Ykä Helariutta, Rupert G Fray
N6-adenosine methylation (m(6) A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6) A writer proteins in Arabidopsis thaliana. The components required for m(6) A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6) A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6) A methylation plays a role in developmental decisions during pattern formation...
May 15, 2017: New Phytologist
https://www.readbyqxmd.com/read/28501491/diagnostic-usefulness-of-xpert-mtb-rif-assay-for-detection-of-tuberculous-meningitis-using-cerebrospinal-fluid
#19
Syed Beenish Rufai, Amit Singh, Jitendra Singh, Parveen Kumar, Manimuthu Mani Sankar, Sarman Singh
OBJECTIVE: Tuberculous meningitis (TBM) is the most severe form of extra-pulmonary tuberculosis (TB) due to association of diseases with high rates of mortality and morbidity. Diagnosis continues to be a clinical challenge as microbiological confirmation is rare and time consuming resulting in delayed treatment. Xpert MTB/RIF assay is a rapid and simple test, which has been endorsed by World Health Organization as an initial diagnostic test for the diagnosis of TBM. However, evidence still lacks for its performance on cerebrospinal fluid (CSF) for the diagnosis of TBM especially from India...
May 10, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28500851/calreticulin-modulates-the-intracellular-survival-of-mycobacteria-by-regulating-er-stress-mediated-apoptosis
#20
Sung Hee Jo, Ji-Ae Choi, Yun-Ji Lim, Junghwan Lee, Soo-Na Cho, Sung-Man Oh, Dam Go, Seon-Hwa Kim, Chang-Hwa Song
Endoplasmic reticulum (ER)-stress-mediated apoptosis is a host defense mechanism against Mycobacterium tuberculosis (Mtb) infection. Calreticulin (CRT) is the major calcium-binding chaperone protein. Previous reports have suggested a close relationship between the cell-surface expression of CRT and apoptosis. In this study, the role of CRT during Mtb infection was examined. The results showed that Mtb infection induces CRT production by macrophages and that CRT levels are correlated with the degree of apoptotic cell death...
April 25, 2017: Oncotarget
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