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https://www.readbyqxmd.com/read/28092821/efficacy-of-vacuum-steam-pasteurization-for-inactivation-of-salmonella-pt-30-escherichia-coli-o157-h7-and-enterococcus-faecium-on-low-moisture-foods
#1
Manoj K Shah, Gladys Asa, Julie Sherwood, Kari Graber, Teresa M Bergholz
Low moisture foods such as nuts, spices, and seeds have been implicated in several outbreaks due to Salmonella or E. coli O157:H7 contamination. Such foods may be consumed raw, and can be used as ingredients in other food products. While numerous thermal inactivation studies have been conducted for Salmonella on nuts, studies on other seeds and grains are minimal. Product water activity can influence the thermal resistance of pathogens, where thermal resistance increases as water activity decreases, leading to a requirement for higher temperatures and longer exposure times to achieve significant reduction of pathogen numbers...
January 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28089736/antibacterial-activity-of-limonium-brasiliense-baicuru-against-multidrug-resistant-bacteria-using-a-statistical-mixture-design
#2
Andressa Blainski, Barbara Gionco, Admilton G Oliveira, Galdino Andrade, Ieda S Scarminio, Denise B Silva, Norberto P Lopes, João C P Mello
ETHNOPHARMACOLOGICAL RELEVANCE: Limonium brasiliense (Boiss.) Kuntze (Plumbaginaceae) is commonly known as "baicuru" or "guaicuru" and preparations of its dried rhizomes have been populary used in the treatment of premenstrual syndrome and menstrual disorder, and as an antiseptic in genito-urinary infections. This study evaluated the potential antibacterial activity of rhizome extracts against multidrug-resistant bacterial strains using statistical mixture design. MATERIALS AND METHODS: The statistical design of four components (water, methanol, acetone and ethanol) produced 15 different extracts and also a confirmatory experiment, which was performed using water:acetone (3:7, v/v)...
January 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28087492/multi-antibiotic-resistant-and-putative-virulence-gene-signatures-in-enterococcus-species-isolated-from-pig-farms-environment
#3
Abeni Beshiru, Isoken H Igbinosa, Faith I Omeje, Abraham G Ogofure, Martin M Eyong, Etinosa O Igbinosa
The continuous misuse of antimicrobials in food animals both orally and subcutaneously as therapeutic and prophylactic agents to bacterial infections could be detrimental and contribute to the dissemination of resistant clones in livestock production. The present study was carried out to determine the antibiogram and virulence gene characteristics of Enterococcus species from pig farms. A total of 300 faecal samples were obtained from two pig farms in Benin City between February and July, 2016. Standard culture-based and polymerase chain reaction (PCR) assay were adopted in the detection and characterization of the Enterococcus species...
January 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28070313/decarboxylase-positive-enterococcus-faecium-strains-isolated-from-rabbit-meat-and-their-sensitivity-to-enterocins
#4
Andrea Lauková, Renata Szabóová, Pavel Pleva, Leona Buňková, Ľubica Chrastinová
BACKGROUND: The objective of the study was to determine sensitivity of Enterococcus faecium strains from rabbit meat to enterocins. RESULTS: Twenty-five decarboxylase-positive strains (from rabbit meat) allotted to the species E. faecium by genotypization and by MALDI TOF MS spectrometry identification (evaluation score value range 2.104-2.359; in the range for highly probable species identification-score value 2.300-3.000 and secure probable species identification/probable species identification-2...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28069645/a-novel-phosphodiesterase-of-the-gdpp-family-modulates-cyclic-di-amp-levels-in-response-to-cell-membrane-stress-in-daptomycin-resistant-enterococci
#5
Xu Wang, Milya Davlieva, Jinnethe Reyes, Diana Panesso, Cesar A Arias, Yousif Shamoo
Substitutions in the LiaFSR membrane stress pathway are frequently associated with emergence of antimicrobial peptide resistance in both Enterococcus faecalis and Enterococcus faecium Cyclic di-AMP (c-di-AMP) is an important signal molecule that affects many aspects of bacterial physiology including stress response. We have identified previously a mutation in a gene (designated yybT) in E. faecalis that was associated with development of daptomycin resistance resulting in a change at position 440 in the predicted protein (yybT(I440S))...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28068600/design-synthesis-and-antibacterial-evaluation-of-novel-pleuromutilin-derivatives-possessing-piperazine-linker
#6
Mei-Ling Gao, Jie Zeng, Xi Fang, Jian Luo, Zhen Jin, Ya-Hong Liu, You-Zhi Tang
A series of pleuromutilin derivatives bearing piperazine ring have been reported. The in vitro antibacterial activities of the synthetic derivatives against MRSA (ATCC 43300), Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Enterococcus faecium (ATCC35667) and Escherichia coli (ATCC25922) were evaluated by the broth dilution method. Most of the synthesized derivatives displayed potent activities. Compounds 11c, 12a and 12c were found to be the most active antibacterial derivatives against MRSA (minimum inhibitory concentration = 0...
January 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28063147/genetic-relatedness-of-faecal-coliforms-and-enterococci-bacteria-isolated-from-water-and-sediments-of-the-apies-river-gauteng-south-africa
#7
Mutshiene Deogratias Ekwanzala, Akebe Luther King Abia, Eunice Ubomba-Jaswa, Jitendra Keshri, Ndombo Benteke Maggy Momba
To date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. Therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of Apies River to better understand the genetic similarity of microorganisms between the sediment and water phases. Indicator bacteria were subjected to a molecular study, which consisted of PCR amplification and sequence analysis of the 16S rRNA and 23S rRNA gene using specific primers for faecal coliforms and enterococci, respectively...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28056219/antimicrobial-resistant-escherichia-coli-and-enterococcus-spp-isolated-from-miranda-donkey-equus-asinus-an-old-problem-from-a-new-source-with-a-different-approach
#8
Isabel Carvalho, Rosa Del Campo, Margarida Sousa, Nuno Silva, João Carrola, Catarina Marinho, Tiago Santos, Silvia Carvalho, Miguel Nóvoa, Miguel Quaresma, José Eduardo Pereira, Marta Cobo, Gilberto Igrejas, Patricia Poeta
The Miranda Donkey (Equus asinus) is an endangered asinine from Miranda do Douro region, located in North East of Portugal. We study the antimicrobial resistance (AMR) and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disk diffusion method. Carriage of genes codifying for antibiotic resistant and virulent factors was analyzed by polymerase chain reaction (PCR)...
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28051225/tigecycline-based-therapy-for-glycopeptide-resistant-enterococcus-faecium-infection-in-a-pediatric-intensive-care-unit
#9
Becem Trabelsi, Mehdi Trifa, Sonia Ben Khalifa
No abstract text is available yet for this article.
June 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/28049149/rela-mutant-enterococcus-faecium-with-multiantibiotic-tolerance-arising-in-an-immunocompromised-host
#10
Erin S Honsa, Vaughn S Cooper, Mohammed N Mhaissen, Matthew Frank, Jessica Shaker, Amy Iverson, Jeffrey Rubnitz, Randall T Hayden, Richard E Lee, Charles O Rock, Elaine I Tuomanen, Joshua Wolf, Jason W Rosch
: Serious bacterial infections in immunocompromised patients require highly effective antibacterial therapy for cure, and thus, this setting may reveal novel mechanisms by which bacteria circumvent antibiotics in the absence of immune pressure. Here, an infant with leukemia developed vancomycin-resistant Enterococcus faecium (VRE) bacteremia that persisted for 26 days despite appropriate antibiotic therapy. Sequencing of 22 consecutive VRE isolates identified the emergence of a single missense mutation (L152F) in relA, which constitutively activated the stringent response, resulting in elevated baseline levels of the alarmone guanosine tetraphosphate (ppGpp)...
January 3, 2017: MBio
https://www.readbyqxmd.com/read/28044272/gene-cloning-and-expression-of-a-glucoside-3-dehydrogenase-from-sphingobacterium-faecium-zjf-d6-and-used-it-to-produce-n-p-nitrophenyl-3-ketovalidamine
#11
Jian-Fen Zhang, Wei-Qing Chen, Hong Chen
In this study, we report the cloning and expression of a functional glucoside 3-dehydrogenase (G3DH) gene from Sphingobacterium faecium ZJF-D6. This gene is 1686 bp in length and encodes a peptide of 562 amino acids. The G3DH gene was successfully expressed in E. coli, and the recombinant enzyme could oxidize glucosides, galactosides and analogues at C-3 position. The sequence and multiple alignment analysis showed that the enzyme has highest identity with G3DHs from Paraglaciecola polaris LMG 21857, Aliiglaciecola lipolytica E3 and Halomonas sp...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28041735/beneficial-effects-of-probiotic-cholesterol-lowering-strain-of-enterococcus-faecium-wefa23-from-infants-on-diet-induced-metabolic-syndrome-in-rats
#12
Fen Zhang, Liang Qiu, Xiongpeng Xu, Zhengqi Liu, Hui Zhan, Xueying Tao, Nagendra P Shah, Hua Wei
The aim of this study was to select probiotic Enterococcus strains which have the potential to improve metabolic syndrome (MS). Ten Enterococcus strains isolated from healthy infants were evaluated for their probiotic properties in vitro, and Enterococcus faecium WEFA23 was selected due to its cholesterol removal ability (1.89 ± 0.07 mg/10(10) cfu), highest glycodeoxycholic acid-hydrolase activity (1.86 ± 0.01 U/mg), and strong adhesion capacity to Caco-2 cells (17.90 ± 0.19%). The safety of E. faecium WEFA23 was verified by acute oral administration in mice, and it was found to have no adverse effects on general health status, bacterial translocation, and gut mucosal histology...
December 29, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/28039271/novel-lnu-g-gene-conferring-resistance-to-lincomycin-by-nucleotidylation-located-on-tn6260-from-enterococcus-faecalis-e531
#13
Xiao-Qing Zhu, Xiao-Ming Wang, Hui Li, Yan-Hong Shang, Yu-Shan Pan, Cong-Ming Wu, Yang Wang, Xiang-Dang Du, Jian-Zhong Shen
OBJECTIVES: To identify a novel putative lincosamide resistance gene determinant in a swine Enterococcus faecalis E531 exhibiting a lincosamide resistance/macrolide susceptibility (L(R)M(S)) phenotype and to determine its location and genetic environment. METHODS: The whole genomic DNA of E. faecalis E531, which tested negative for the known lincosamide nucleotidyltransferase genes, was sequenced. A putative lincosamide resistance gene determinant was cloned into an Escherichia coli-E...
December 30, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28039075/comparative-genome-analysis-of-multiple-vancomycin-resistant-enterococcus-faecium-isolated-from-two-fatal-cases
#14
Shu Yong Lim, Kien-Pong Yap, Cindy Shuan Ju Teh, Kartini Abdul Jabar, Kwai Lin Thong
Enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. The increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. The aim of this study was to perform comparative genome analyses on four vancomycin-resistant Enterococcus faecium (VREfm) strains isolated from two fatal cases in a tertiary hospital in Malaysia. Two sequence types, ST80 and ST203, were identified which belong to the clinically important clonal complex (CC) 17...
December 28, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28036312/antimicrobial-resistance-of-selected-invasive-bacteria-in-a-tertiary-care-center-results-of-a-prospective-surveillance-study
#15
Olivera Djuric, Snezana Jovanovic, Branka Stosovic, Tanja Tosic, Milica Jovanovic, Ljiljana Markovic-Denic
INTRODUCTION: We aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the Clinical Center of Serbia (CCS) in Belgrade. METHODOLOGY: A total of 477 clinical blood stream isolates of Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter spp. were collected in the period from January to December 2013...
December 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28031272/polyclonal-emergence-of-vana-vancomycin-resistant-enterococcus-faecium-in-australia
#16
Sebastiaan J van Hal, Björn A Espedido, Geoffrey W Coombs, Benjamin P Howden, Tony M Korman, Graeme R Nimmo, Iain B Gosbell, Slade O Jensen
OBJECTIVES: To investigate the genetic context associated with the emergence of vanA VRE in Australia. METHODS: The whole genomes of 18 randomly selected vanA-positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. RESULTS: In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn1546 variants and plasmid backbone structures...
December 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28024867/enterococcus-spp-in-a-single-blood-culture-bacteremia-or-contamination
#17
R Khatib, V Labalo, M Sharma, L B Johnson, K Riederer
We retrospectively evaluated adult cases with Enterococcus spp. in 1 blood culture (BC) (1/1/2010-12/31/2015; n=294) and stratified them into bacteremia or contamination. Contamination frequency was similar in community versus hospital-onset, E. faecalis versus E. faecium, and number of BC drawn per day. Contamination predictors were vancomycin-resistance, ampicillin-resistance, commensal organism copresence, and nonurinary/abdominal sources.
December 15, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28010058/-epidemiology-and-risk-factors-of-patients-with-intra-abdominal-postsurgical-infection-treated-with-tigecycline-a-cohort-study
#18
G Torres, M Paredes, A Hernández, C García, F Sánchez Bueno, M Canteras, P Parrilla, J Gómez
OBJECTIVE: To study a cohort of patients with intra-abdominal postsurgical infection treated with tigecycline to analyze its effectiveness and mortality related factors. METHODS: Prospective study of patients with intra-abdominal postsurgical infection with microbiological isolation and treated with tigecycline. RESULTS: Out of 103 patients only 61 full fit inclusion criteria. Mean age was 67 year-old and 72% were male. Charlson score was ≥ 3 in 65...
December 23, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/27995406/effects-of-ex-vivo-infection-with-etec-on-jejunal-barrier-properties-and-cytokine-expression-in-probiotic-supplemented-pigs
#19
Ulrike Lodemann, Salah Amasheh, Judith Radloff, Martina Kern, Astrid Bethe, Lothar H Wieler, Robert Pieper, Jürgen Zentek, Jörg R Aschenbach
BACKGROUND AND AIM: Enterotoxigenic Escherichia coli (ETEC) strains are involved in piglet post-weaning diarrhea. Prophylactic measures including probiotics have been examined in infection experiments with live piglets. In the present study, we have tested whether the early effects of ETEC infection can also be evoked and studied in a model in which ETEC is added to whole mucosal tissues ex vivo, and whether this response can be modulated by prior supplementation of the piglets with probiotics...
December 19, 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/27994476/a-novel-combination-approach-of-human-polyclonal-ivig-and-antibiotics-against-multidrug-resistant-gram-positive-bacteria
#20
Mariam Madkour Sallam, Khaled Abou-Aisha, Mohamed El-Azizi
BACKGROUND: Gram-positive bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA) and enterococci, have shown a remarkable ability to develop resistance to antimicrobial agents. OBJECTIVE: We aimed to assess possible enhancement of the antimicrobial activity of vancomycin, amoxicillin, clarithromycin, and azithromycin by human polyclonal intravenous immunoglobulin G (IVIG) against 34 multidrug-resistant (MDR) bacterial isolates, including MRSA, Enterococcus faecium, and Enterococcus faecalis...
2016: Infection and Drug Resistance
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