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https://www.readbyqxmd.com/read/28735309/safety-evaluation-of-enterocin-producer-enterococcus-sp-strains-isolated-from-traditional-turkish-cheeses
#1
Mine Avcı, Banu Özden Tuncer
The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Multiple enterocin structural genes were found in 7 strains...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735051/telavancin-activity-in-vitro-tested-against-a-worldwide-collection-of-gram-positive-clinical-isolates-2014
#2
Leonard R Duncan, Helio S Sader, Jennifer I Smart, Robert K Flamm, Rodrigo E Mendes
OBJECTIVES: Telavancin activity was measured against Gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. The results were compared with previous reports. METHODS: A total of 10 552 isolates from 86 sites located in 32 countries were included as part of the Telavancin International Surveillance Program for the Americas, Europe and Asia-Pacific. RESULTS: Telavancin had MIC50 and MIC90 values of 0...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28733126/in-vitro-stepwise-selection-of-reduced-susceptibility-to-lipoglycopeptides-in-enterococci
#3
Francis F Arhin, David Lalonde Seguin, Adam Belley, Gregory Moeck
The propensity of oritavancin to select for stably elevated oritavancin minimum inhibitory concentrations (MICs) was studied by serial passaging of strains in broth containing oritavancin for 20days. Seven clinical strains of Enterococcus faecalis and E. faecium were studied; they included vancomycin-susceptible and both VanA and VanB vancomycin-resistant isolates. Stepwise oritavancin selection yielded stably elevated oritavancin MICs in six of the seven strains, with MIC increases ranging from 4-32-fold. By comparison, stepwise selection with comparator agents dalbavancin (4- to >128-fold MIC increases), telavancin (4-8-fold MIC increases) and daptomycin (4-32-fold MIC increases) also yielded selectants with elevated MICs of the respective agents...
July 1, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28727951/in-vitro-activity-of-telavancin-against-clinically-important-gram-positive-pathogens-from-69-u-s-medical-centers-2015-potency-analysis-by-u-s-census-divisions
#4
Michael A Pfaller, Helio S Sader, Robert K Flamm, Mariana Castanheira, Jennifer I Smart, Rodrigo E Mendes
A total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 U.S. Census Divisions during the 2015 SENTRY Antimicrobial Surveillance Program were included. Telavancin had minimal inhibitory concentration (MIC)50 and MIC90 values of 0.03/0.06 μg/mL, respectively, against methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Similar activity was also observed among coagulase-negative staphylococci (MIC50/90, 0.03/0.06 μg/ml; 100.0% inhibited by ≤0...
July 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28724953/antimicrobial-efficacy-and-synergy-of-metal-ions-against-enterococcus-faecium-klebsiella-pneumoniae-and-acinetobacter-baumannii-in-planktonic-and-biofilm-phenotypes
#5
Misha Y Vaidya, Andrew J McBain, Jonathan A Butler, Craig E Banks, Kathryn A Whitehead
The effects of metal ion solutions (silver, copper, platinum, gold and palladium) were determined individually and in combination against Enterococcus faecium, Acinetobacter baumannii and Klebsiella pneumoniae. Platinum, gold and palladium showed the greatest antimicrobial efficacy in zone of inhibition (ZoI) assays. When tested in combinations using ZoI assays, gold/platinum, gold/palladium and platinum/palladium were indicative of synergy. Microbial inhibitory concentration demonstrated platinum and gold against Enterococcus faecium, platinum against Klebsiella pneumoniae and platinum and silver against Acinetobacter baumannii were optimal...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28715766/different-effects-of-matrix-degrading-enzymes-towards-biofilms-formed-by-e-faecalis-and-e-faecium-clinical-isolates
#6
Riccardo Torelli, Margherita Cacaci, Massimiliano Papi, Francesco Paroni Sterbini, Cecilia Martini, Brunella Posteraro, Valentina Palmieri, Marco De Spirito, Maurizio Sanguinetti, Francesca Bugli
E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates...
July 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28715758/defence-strategies-and-antibiotic-resistance-gene-abundance-in-enterococci-under-stress-by-exposure-to-low-doses-of-peracetic-acid
#7
Andrea Turolla, Raffaella Sabatino, Diego Fontaneto, Ester M Eckert, Noemi Colinas, Gianluca Corno, Barbara Citterio, Francesca Biavasco, Manuela Antonelli, Alessandro Mauro, Gianmarco Mangiaterra, Andrea Di Cesare
Peracetic acid (PAA) is an organic compound used efficiently as disinfectant in wastewater treatments. Yet, at low doses it may cause selection; thus, the effect of low doses of PAA on Enterococcus faecium as a proxy of human-related microbial waste was evaluated. Bacteria were treated with increasing doses of PAA (from 0 to 25 mg L(-1) min) and incubated in regrowth experiments under non-growing, limiting conditions and under growing, favorable conditions. The changes in bacterial abundance, in bacterial phenotype (number and composition of small cell clusters), and in the abundance of an antibiotic resistance gene (ARG) was evaluated...
July 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/28708453/virulence-and-antibiotic-resistance-of-enterococci-isolated-from-healthy-breastfed-infants
#8
José M Landete, Ángela Peirotén, Margarita Medina, Juan L Arqués, Eva Rodríguez-Mínguez
Pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. Most studies to date in enterococci from healthy infants have been focused on Enterococcus faecalis, despite the growing concern about nosocomial infections caused by E. faecium. In this work, we studied the antibiotic resistance and virulence determinants of 26 E. faecalis and 15 E. faecium intestinal isolates from Spanish healthy breastfed infants. Overall, commensal enterococci studied contained antibiotic resistance and virulence genes, although their patterns were not according to those described for antibiotic-resistant hospital-associated enterococci...
July 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28701170/antimicrobial-susceptibility-among-gram-positive-and-gram-negative-organisms-collected-from-the-latin-american-region-between-2004-and-2015-as-part-of-the-tigecycline-evaluation-and-surveillance-trial
#9
Silvio Vega, Michael J Dowzicky
BACKGROUND: The in vitro activity of tigecycline and comparator agents was evaluated against Gram-positive and Gram-negative isolates collected in Latin American centers between 2004 and 2015 as part of the Tigecycline Evaluation and Surveillance Trial (T.E.S.T.) global surveillance study. METHODS: Minimum inhibitory concentrations (MICs) were determined using the broth microdilution methodology according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
July 12, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28698020/first-outbreak-of-colonization-by-linezolid-and-glycopeptide-resistant-enterococcus-faecium-harbouring-the-cfr-gene-in-a-uk-nephrology-unit
#10
Teresa Inkster, John Coia, Danièle Meunier, Michel Doumith, Kate Martin, Rachel Pike, Laura Imrie, Hayley Kane, Marlene Hay, Camilla Wiuff, Julie Wilson, Chris Deighan, Katie L Hopkins, Neil Woodford, Robert Hill
AIM: To describe an outbreak of colonization by linezolid- and glycopeptide-resistant Enterococcus faecium harbouring the cfr gene, in a UK nephrology unit. METHODS: Isolates of linezolid-resistant E. faecium were typed by PFGE and examined by PCR and sequencing for the transmissible cfr gene that confers resistance to linezolid. Enhanced environmental cleaning, initial and weekly screening of all patients and monitoring of adherence to standard infection control precautions (SICPs) were implemented...
July 8, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28694023/reactivity-toward-bifidobacterium-longum-and-enterococcus-hirae-demonstrate-robust-cd8-t-cell-response-and-better-prognosis-in-hbv-related-hepatocellular-carcinoma
#11
Yihui Rong, Zheng Dong, Zhixian Hong, Yun Jin, Wei Zhang, Bailong Zhang, Wei Mao, Huifang Kong, Chunping Wang, Bin Yang, Xudong Gao, Zhenyu Song, Susan E Green, Haihan K Song, Hongbo Wang, Yinying Lu
Recent studies suggest that several bacterial species are involved in tumor immunosurveillance and antitumor immunity. The role of bacteria in immune responses in HBV-related hepatocellular carcinoma (HCC) patients is still unknown. In this study, we examined the bacteria-reactive CD8(+) T cell response in patients with HBV-related HCC. We found that circulating CD8(+) T cells from healthy individuals demonstrated minimal or zero specificity toward a series of commensals and bacteria previously associated with antitumor effects, including Escherichia coli, Enterococcus faecium, Bifidobacterium longum, Bacteroides fragilis, and Enterococcus hirae...
July 7, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28693665/characterization-of-high-level-ampicillin-and-aminoglycoside-resistant-enterococci-isolated-from-non-hospital-sources
#12
Emmanuel O Ngbede, Mashood A Raji, Clara N Kwanashie, Jacob K P Kwaga, Alex A Adikwu, Nanven A Maurice, Andrew M Adamu
PURPOSE: High level ampicillin- and aminoglycoside-resistant enterococci are being increasingly reported from non-hospital sources. This study was carried out to characterize these strains from non-hospital sources in Nigeria. METHODOLOGY: A collection of Enterococcus faecium isolated from vegetables, soil, farm animals and manure and observed to be resistant to ampicillin (n=63) and gentamicin (n=37) discs, were screened for resistance to high levels of ampicillin and aminoglycoside using E-test strips...
July 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28680588/incidence-clinical-characteristics-and-outcomes-of-nosocomial-enterococcus-spp-bloodstream-infections-in-a-tertiary-care-hospital-in-beijing-china-a-four-year-retrospective-study
#13
Yangyang Zhang, Mingmei Du, Yan Chang, Liang-An Chen, Qing Zhang
BACKGROUND: Enterococcus spp. are the common cause of nosocomial bloodstream infections (BSIs) with high morbidity and mortality. The purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal BSIs at a large Chinese tertiary-care hospital in Beijing, China. METHODS: A retrospective cohort study on adult patients with nosocomial BSIs due to Enterococcus spp. was performed between January 1, 2012, and December 31, 2015 at the Chinese People's Liberation Army General Hospital...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28678976/probiotic-therapy-reduces-inflammation-and-improves-intestinal-morphology-in-rats-with-induced-oral-mucositis
#14
Dayana Gerhard, Frederico José da Silva Simão de Sousa, Rodrigo Antonio Carvalho Andraus, Paulo Eduardo Pardo, Gisele Alborguetti Nai, Hermann Bremer Neto, Michel Reis Messora, Luciana Prado Maia
The aim of the present study was to evaluate the effect of systemic administration of probiotics (PROB) on the progression of experimentally induced oral and intestinal mucositis in rats immunosuppressed by chemotherapy (5-fluorouracil: 5-FU). Twenty-four rats were divided into the following groups (n=6): GC (control), GPROB, G5FU and G5-FU/PROB. Groups GPROB and G5-FU/PROB received 1 g of probiotic incorporated into each 100 g of feed (Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacilllus acidophilus), beginning 30 days before oral mucositis induction...
July 3, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28676409/enterococci-in-orthopaedic-infections-who-is-at-risk-getting-infected
#15
Ilker Uçkay, Daniela Pires, Americo Agostinho, Nastassia Guanziroli, Mehmet Öztürk, Placido Bartolone, Philippe Tscholl, Michael Betz, Didier Pittet
Some orthopaedic patients might be at risk for enterococcal infections and might benefit from adapted perioperative prophylaxis. METHODS: We performed a single-center cohort of adult patients with orthopaedic infections. RESULTS: Among 2740 infection episodes, 665 surgeries (24%) involved osteosynthesis material, including total joint arthroplasties. The recommended perioperative prophylaxis was cefuroxime (or vancomycin in case of documented MRSA body carriage)...
July 1, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28676009/aging-eye-microbiota-in-dry-eye-syndrome-in-patients-treated-with-enterococcus-faecium-and-saccharomyces-boulardii
#16
Giuseppe Chisari, Eleonora Margherita Chisari, Antonio Maria Borzì, Erdogan Ozyalcin, Clara Grazia Chisari
Aging seem to have a key role in the onset and progression of ocular surface diseases. Dry Eye Syndrome (DES) is a multifactorial disease of the tears and ocular surface in which symptoms may interfere with the ability to work and carry out daily functions. METHODS: This clinical trial was a pilot study to evaluate the effects of supplementation with mixture (MYA796 and Enterococcus faecium SGEf01) on the tear film. Following the run-in period subjects were randomized in two groups: group A (n...
July 4, 2017: Current Clinical Pharmacology
https://www.readbyqxmd.com/read/28675109/titanium-surfaces-immobilized-with-the-major-antimicrobial-fragment-fk-16-of-human-cathelicidin-ll-37-are-potent-against-multiple-antibiotic-resistant-bacteria
#17
Biswajit Mishra, Guangshun Wang
Infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. Antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. Previously, the authors' laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17BIPHE2), a peptide engineered based on the only human cathelicidin. This study documents an alternative solution via covalent immobilization of FK-16, amino acid sequence FKRIVQRIKDFLRNLV-amide, which corresponds to the major antimicrobial region (residues 17-32) of LL-37...
July 4, 2017: Biofouling
https://www.readbyqxmd.com/read/28670644/carriage-of-multidrug-resistant-enterococcus-faecium-and-enterococcus-faecalis-among-apparently-healthy-humans
#18
Solayide A Adesida, Cynthia C Ezenta, Ajoke O Adagbada, Amudat A Aladesokan, Akitoye O Coker
BACKGROUND: Enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. Recently, interest in two major species, E. faecium and E. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. This study was aimed at determining the prevalence of E. faecium and E. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics. MATERIALS AND METHODS: One hundred faecal samples were collected from apparently healthy individuals and analysed using conventionalbacteriological methods...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28668727/inactivation-of-viruses-and-bacteria-on-strawberries-using-a-levulinic-acid-plus-sodium-dodecyl-sulfate-based-sanitizer-taking-sensorial-and-chemical-food-safety-aspects-into-account
#19
Zijin Zhou, Sophie Zuber, Frédérique Cantergiani, Sophie Butot, Dan Li, Thomas Stroheker, Frank Devlieghere, Anthony Lima, Umberto Piantini, Mieke Uyttendaele
The efficacy of levulinic acid (LVA) in combination with sodium dodecyl sulfate (SDS) in removal of foodborne viruses, enteric bacterial pathogens and their surrogates on fresh strawberries was investigated. Inoculated strawberries were treated with potable water, sodium hypochlorite solution (50ppm), 0.5% LVA plus 0.5% SDS solution, and 5% LVA plus 2% SDS solution respectively for 2min, followed by spray-rinsing with potable water. Water washing removed at least 1.0-log of the tested viral and bacterial strains from the strawberries' surfaces...
June 23, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28663538/urinary-tract-infections-after-kidney-transplantation-a-risk-factor-analysis-of-417-patients
#20
Liselotte Ooms, Jan IJzermans, Anne Voor In 't Holt, Michiel Betjes, Margreet Vos, Turkan Terkivatan
BACKGROUND The aim of this study was to evaluate the number of urinary tract infections (UTI) that occur after kidney transplantation (KT) and to identify possible risk factors for development of a UTI. MATERIAL AND METHODS We retrospectively analyzed all KTs performed between January 2012 and December 2013 in the Erasmus University Medical Center, Rotterdam. UTI was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacterium of ≥10^5 CFU/mL, (3) which was treated with antibiotics, and (4) which occurred within 3 months after KT...
June 30, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
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