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https://www.readbyqxmd.com/read/28812171/complete-genome-sequence-of-a-novel-virulent-ahjdlikevirus-bacteriophage-that-infects-enterococcus-faecium
#1
Shaozhen Xing, Xianglilan Zhang, Qiang Sun, Jian Wang, Zhiqiang Mi, Guangqian Pei, Yong Huang, Xiaoping An, Kaifei Fu, Lijun Zhou, Baohua Zhao, Yigang Tong
A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats...
August 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28810329/-distribution-and-drug-resistance-of-pathogens-at-hematology-department-of-jiangsu-province-from-2014-to-2015-results-from-a-multicenter-retrospective-study
#2
Y K Wan, W Sang, B Chen, Y G Yang, L Q Zhang, A N Sun, Y J Liu, Y Xu, Y P Cai, C B Wang, Y F Shen, Y W Jiang, X Y Zhang, W Xu, M Hong, T Chen, R R Xu, F Li, Y L Xu, Y Xue, Y L Lu, Z M He, W M Dong, Z Chen, M H Ji, Y Y Yang, L J Zhai, Y Zhao, G Q Wu, J H Ding, J Cheng, W B Cai, Y M Sun, J Ouyang
Objective: To describe the distribution and drug resistance of pathogens at hematology department of Jiangsu Province from 2014 to 2015 to provide reference for empirical anti-infection treatment. Methods: Pathogens were from hematology department of 26 tertiary hospitals in Jiangsu Province from 2014 to 2015. Antimicrobial susceptibility testing was carried out according to a unified protocol using Kirby-Bauer method or agar dilution method. Collection of drug susceptibility results and corresponding patient data were analyzed...
July 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28807390/vancomycin-resistant-enterococcus-faecium-st17-outbreak-in-a-neonatal-unit
#3
Luis López-Urrutia, Mónica de Frutos, Jesús Oteo, José María Eiros
No abstract text is available yet for this article.
August 11, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28799224/genomic-insights-into-the-pathogenicity-and-environmental-adaptability-of-enterococcus-hirae-r17-isolated-from-pork-offered-for-retail-sale
#4
Zixin Peng, Menghan Li, Wei Wang, Hongtao Liu, Séamus Fanning, Yujie Hu, Jianzhong Zhang, Fengqin Li
Genetic information about Enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. In this study, comparative analysis was performed of E. hirae R17, a daptomycin-resistant strain isolated from pork purchased from a retail market in Beijing, China, and three other enterococcal genomes (Enterococcus faecium DO, Enterococcus faecalis V583, and E. hirae ATCC(™) 9790). Some 1,412 genes were identified that represented the core genome together with an additional 139 genes that were specific to E...
August 10, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28799106/chemical-sterilization-of-allograft-dermal-tissues
#5
Abigail Phipps, Edward Vaynshteyn, John B Kowalski, Manh-Dan Ngo, Karen Merritt, Joel Osborne, Evangelia Chnari
Common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. One approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. This combination of processes is an accepted method for production of sterile healthcare products as described in ANSI/AAMI ST67:2011. This article describes the validation of the peracetic acid and ethanol-based (PAAE) chemical sterilization process for allograft dermal tissues at the Musculoskeletal Transplant Foundation (MTF, Edison, NJ)...
August 10, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28783248/coupling-metagenomics-with-cultivation-to-select-host-specific-probiotic-microorganisms-for-subtropical-aquaculture
#6
Jinming Cui, Minfeng Xiao, Mingzhu Liu, Zuowei Wang, Furong Liu, Liang Guo, Hailin Meng, Honggang Zhang, Jinfang Yang, Deng Deng, Shuangqiang Huang, Yingfei Ma, Chenli Liu
AIMS: To demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry. METHODS AND RESULTS: By using both culture-dependent and -independent methods, we have systematically investigated, for the first time, the gut microbiota of twelve subtropical aquatic animal species. We found that the diversity, abundance, and distribution of gut microorganisms of these animals were host-specific, and that lactic acid bacteria (LAB) were predominant among the indigenous probiotic microbes...
August 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28780877/-identification-of-risk-factors-of-multiresistant-infections-on-two-intensive-care-units
#7
Marcell Szabó, Noémi Kanász, Katalin Darvas, János Gál
INTRODUCTION: Intensive care units are favourable environment for infections, many of them are caused by antibiotic resistant bacteria. AIM: Identifying risk factors of ICU-acquired multiresistant infections. METHOD: We performed observational study on two academic intensive care units (a multidisciplinary and a surgical ICU) between 01/09/2014 and 30/11/2015. Patients with a first infection caused by predefined organisms (P. aeruginosa, E...
August 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/28780309/clinical-and-microbiological-characteristics-of-pantoea-agglomerans-infection-in-children
#8
Ayşe Büyükcam, Özlem Tuncer, Deniz Gür, Banu Sancak, Mehmet Ceyhan, Ali Bülent Cengiz, Ateş Kara
Pantoea agglomerans is an environmental Gram-negative bacterium that rarely is responsible for the infections in humans but it is often a causative factor of a number of occupational diseases. This study evaluated the clinical and microbiological characteristics and pathogenicity of P. agglomerans in children. We retrospectively reviewed microbiological test results for all children (1 month old to 18 years old) who were admitted to our pediatric hospital between January 2000 to June 2015 and had positive clinical specimen cultures for P...
August 2, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28774148/synthesis-and-structural-characterization-of-silver-nanoparticles-stabilized-with-3-mercapto-1-propansulfonate-and-1-thioglucose-mixed-thiols-for-antibacterial-applications
#9
Francesco Porcaro, Laura Carlini, Andrea Ugolini, Daniela Visaggio, Paolo Visca, Ilaria Fratoddi, Iole Venditti, Carlo Meneghini, Laura Simonelli, Carlo Marini, Wojciech Olszewski, Nitya Ramanan, Igor Luisetto, Chiara Battocchio
The synthesis, characterization and assessment of the antibacterial properties of hydrophilic silver nanoparticles (AgNPs) were investigated with the aim to probe their suitability for innovative applications in the field of nanobiotechnology. First, silver nanoparticles were synthetized and functionalized with two capping agents, namely 3-mercapto-1-propansulfonate (3MPS) and 1-β-thio-d-glucose (TG). The investigation of the structural and electronic properties of the nano-systems was carried out by means of X-ray Photoelectron Spectroscopy (XPS) and X-ray Absorption Spectroscopy (XAS)...
December 20, 2016: Materials
https://www.readbyqxmd.com/read/28771110/temporary-intra-operative-portocaval-shunts-post-operative-infections-and-mid-term-survival-after-cava-sparing-liver-transplantation
#10
Maximilian Weniger, Joachim Andrassy, Thomas Weig, Beatrice Grabein, Alexander Crispin, Markus Rentsch, Christian Siebers, Alexandr Bazhin, Jan G D'Haese, Werner Hartwig, Jens Werner, Markus Guba, Eugen Faist, Sebastian Pratschke, Martin K Angele
BACKGROUND: Temporary intra-operative portocaval shunts (TPCS) are believed to improve outcomes after cava-sparing liver transplantation. We hypothesize that decompression of the portal venous system via a TPCS reduces gut congestion, thereby decreasing bacterial translocation. Thus, we sought to clarify whether transplantation with a TPCS alters rates of post-operative infections and survival. PATIENTS AND METHODS: Patients undergoing liver transplantation (n = 189) were stratified by usage of a TPCS and the type of intra-operative antibiotic prophylaxis...
August 3, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28764160/revisiting-nitrofurantoin-for-vancomycin-resistant-enterococci
#11
Suneeta Meena, Sarita Mohapatra, Seema Sood, Benu Dhawan, Bimal Ku Das, Arti Kapil
INTRODUCTION: Enterococcal infection has emerged as a major therapeutic challenge. Emergence of High Level Aminoglycoside Resistance (HLAR) and Vancomycin-Resistant Enterococcus (VRE) has further limited the drug therapy in enterococcal infections. However, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents. AIM: To detect susceptibility of nitrofurantoin against VRE isolates from Urinary Tract Infection (UTI) of outdoor and indoor patient departments...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28763660/vancomycin-resistant-enterococci-with-vana-gene-in-treated-municipal-wastewater-and-their-association-with-human-hospital-strains
#12
Veronika Oravcova, Matus Mihalcin, Jana Zakova, Lucie Pospisilova, Martina Masarikova, Ivan Literak
Vancomycin-resistant enterococci (VRE) are pathogens of increasing medical importance. In Brno, Czech Republic, we collected 37 samples from the effluent of a wastewater treatment plant (WWTP), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. Moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the WWTP. Samples were cultured selectively. Enterococci were identified using MALDI-TOF MS, phenotypically tested for susceptibility to antibiotics, and by PCR for occurrence of resistance and virulence genes...
July 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28759747/clonal-dissemination-of-vancomycin-resistant-enterococcus-faecium-st412-in-a-brazilian-region
#13
Geraldo da Silva Alves, Monalessa Fábia Pereira, Lais de Lima Bride, Ana Paula Ferreira Nunes, Ricardo Pinto Schuenck
Vancomycin-resistant Enterococcus faecium (VREfm) has emerged as an important global nosocomial pathogen, and this trend is associated with the spread of high-risk clones. Here, we determined the genetic and phenotypic features of 93 VREfm isolates that were obtained from patients in 13 hospitals in Vitória, Espírito Santo, Brazil, during 2012-2013. All the isolates were vancomycin-resistant and harbored the vanA gene. Only 6 (6.5%) of the VREfm isolates showed the ability to form biofilm. The 93 isolates analyzed belong to a single pulsed-field gel electrophoresis lineage and presented six subtypes...
July 28, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28754459/first-description-in-europe-of-emergence-of-enterococcus-faecium-st117-carrying-both-vana-vanb-genes-isolated-in-greece
#14
C C Papagiannitsis, E Malli, Z Florou, M Medvecky, S Sarrou, J Hrabak, E Petinaki
OBJECTIVES: An Enterococcus faecium, carrying both vanA/vanB genes was recovered from a patient with bacteraemia, treated in a Greek hospital. Since this is the first description in Europe of E. faecium, carrying both vanA/vanB genes, the isolate was further studied. METHODS: Susceptibility to several antibiotics was determined by automated system Vitek 2. The isolate was typed by MLST. To define the genetic units of the vanA and vanB genes, plasmid-content of strain was analyzed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease following by hybridization with digoxigenin-labelled vanA and vanB probes...
July 25, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28746396/recovery-of-metallo-tolerant-and-antibiotic-resistant-psychrophilic-bacteria-from-siachen-glacier-pakistan
#15
Muhammad Rafiq, Muhammad Hayat, Alexandre M Anesio, Syed Umair Ullah Jamil, Noor Hassan, Aamer Ali Shah, Fariha Hasan
Cultureable bacterial diversity of previously unexplored Siachen glacier, Pakistan, was studied. Out of 50 isolates 33 (66%) were Gram negative and 17 (34%) Gram positive. About half of the isolates were pigment producers and were able to grow at 4-37°C. 16S rRNA gene sequences revealed Gram negative bacteria dominated by Proteobacteria (especially γ-proteobacteria and β-proteobacteria) and Flavobacteria. The genus Pseudomonas (51.51%, 17) was dominant among γ- proteobacteria. β-proteobacteria constituted 4 (12...
2017: PloS One
https://www.readbyqxmd.com/read/28743946/identification-of-the-eskape-pathogens-by-mass-spectrometric-analysis-of-microbial-membrane-glycolipids
#16
Lisa M Leung, William E Fondrie, Yohei Doi, J Kristie Johnson, Dudley K Strickland, Robert K Ernst, David R Goodlett
Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28735309/safety-evaluation-of-enterocin-producer-enterococcus-sp-strains-isolated-from-traditional-turkish-cheeses
#17
Mine Avcı, Banu Özden Tuncer
The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Multiple enterocin structural genes were found in 7 strains...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735051/telavancin-activity-in-vitro-tested-against-a-worldwide-collection-of-gram-positive-clinical-isolates-2014
#18
Leonard R Duncan, Helio S Sader, Jennifer I Smart, Robert K Flamm, Rodrigo E Mendes
OBJECTIVES: Telavancin activity was measured against Gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. The results were compared with previous reports. METHODS: A total of 10552 isolates from 86 sites located in 32 countries were included as part of the Telavancin International Surveillance Program for the Americas, Europe and Asia-Pacific. RESULTS: Telavancin had MIC50 and MIC90 values of 0...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28733126/in-vitro-stepwise-selection-of-reduced-susceptibility-to-lipoglycopeptides-in-enterococci
#19
Francis F Arhin, David Lalonde Seguin, Adam Belley, Gregory Moeck
The propensity of oritavancin to select for stably elevated oritavancin minimum inhibitory concentrations (MICs) was studied by serial passaging of strains in broth containing oritavancin for 20days. Seven clinical strains of Enterococcus faecalis and E. faecium were studied; they included vancomycin-susceptible and both VanA and VanB vancomycin-resistant isolates. Stepwise oritavancin selection yielded stably elevated oritavancin MICs in six of the seven strains, with MIC increases ranging from 4-32-fold. By comparison, stepwise selection with comparator agents dalbavancin (4- to >128-fold MIC increases), telavancin (4-8-fold MIC increases) and daptomycin (4-32-fold MIC increases) also yielded selectants with elevated MICs of the respective agents...
July 1, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28727951/in-vitro-activity-of-telavancin-against-clinically-important-gram-positive-pathogens-from-69-u-s-medical-centers-2015-potency-analysis-by-u-s-census-divisions
#20
Michael A Pfaller, Helio S Sader, Robert K Flamm, Mariana Castanheira, Jennifer I Smart, Rodrigo E Mendes
A total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 U.S. Census Divisions during the 2015 SENTRY Antimicrobial Surveillance Program were included. Telavancin had minimal inhibitory concentration (MIC)50 and MIC90 values of 0.03/0.06 μg/mL, respectively, against methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Similar activity was also observed among coagulase-negative staphylococci (MIC50/90, 0.03/0.06 μg/ml; 100.0% inhibited by ≤0...
July 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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