keyword
https://read.qxmd.com/read/22990970/the-zinc-finger-protein-tczfp2-binds-target-mrnas-enriched-during-trypanosoma-cruzi-metacyclogenesis
#21
JOURNAL ARTICLE
Patricia Alves Mörking, Rita de Cássia Pontello Rampazzo, Pegine Walrad, Christian Macagnan Probst, Maurilio José Soares, Daniela Fiori Gradia, Daniela Parada Pavoni, Marco Aurélio Krieger, Keith Matthews, Samuel Goldenberg, Stenio Perdigão Fragoso, Bruno Dallagiovanna
Trypanosomes are parasitic protozoa in which gene expression is primarily controlled through the regulation of mRNA stability and translation. This post-transcriptional control is mediated by various families of RNA-binding proteins, including those with zinc finger CCCH motifs. CCCH zinc finger proteins have been shown to be essential to differentiation events in trypanosomatid parasites. Here, we functionally characterise TcZFP2 as a predicted post-transcriptional regulator of differentiation in Trypanosoma cruzi...
September 2012: Memórias do Instituto Oswaldo Cruz
https://read.qxmd.com/read/21944888/melatonin-and-zinc-treatment-distinctive-modulation-of-cytokine-production-in-chronic-experimental-trypanosoma-cruzi-infection
#22
JOURNAL ARTICLE
Vânia Brazão, Marina Del Vecchio Filipin, Fabricia Helena Santello, Leony Cristina Caetano, Ana Amélia Carraro Abrahão, Míriam Paula Alonso Toldo, José Clóvis do Prado
Melatonin by exhibiting antioxidant, anti-aging, and immunomodulatory properties favorably modulate the immune function, protecting the hosts from several infectious diseases. Zinc is an essential trace element important for the efficiency of the immune system in reason of its widespread role in the activity of enzymes, transcription factors and cytokines. The etiology of Chagas' disease, caused by a protozoan parasite Trypanosoma cruzi, has been the focus of considerable discussion, although chronic phase still remains not fully understood...
December 2011: Cytokine
https://read.qxmd.com/read/18793339/the-molecular-analysis-of-trypanosoma-cruzi-metallocarboxypeptidase-1-provides-insight-into-fold-and-substrate-specificity
#23
JOURNAL ARTICLE
Gabriela Niemirowicz, Daniel Fernández, Maria Solà, Juan J Cazzulo, Francesc X Avilés, F Xavier Gomis-Rüth
Trypanosoma cruzi is the aetiological agent of Chagas' disease, a chronic infection that affects millions in Central and South America. Proteolytic enzymes are involved in the development and progression of this disease and two metallocarboxypeptidases, isolated from T. cruzi CL Brener clone, have recently been characterized: TcMCP-1 and TcMCP-2. Although both are cytosolic and closely related in sequence, they display different temporary expression patterns and substrate preferences. TcMCP-1 removes basic C-terminal residues, whereas TcMCP-2 prefers hydrophobic/aromatic residues...
November 2008: Molecular Microbiology
https://read.qxmd.com/read/18599007/purification-and-subcellular-localization-of-a-secreted-75-kda-trypanosoma-cruzi-serine-oligopeptidase
#24
JOURNAL ARTICLE
Raquel Elisa da Silva-Lopez, José Andrés Morgado-Díaz, Priscila Tavares dos Santos, Salvatore Giovanni-De-Simone
An extracellular serine peptidase was purified 460-fold from Trypanosoma cruzi epimastigotes culture supernatant with (NH(4))(2)SO(4) precipitation followed by affinity chromatography aprotinin-agarose and continuous elution electrophoresis, yielding a total recovery of 65%. The molecular mass of the active enzyme estimated by reducing and non-reducing SDS-PAGE was about 75kDa. The optimal pH and temperature of this glycosylated peptidase were 8.0 and 37 degrees C using alpha-N-rho-tosyl-L-arginine-methyl ester (L-TAME) as substrate...
August 2008: Acta Tropica
https://read.qxmd.com/read/18394811/zinc-supplementation-increases-resistance-to-experimental-infection-by-trypanosoma-cruzi
#25
JOURNAL ARTICLE
Vânia Brazão, Leony Cristina Caetano, Marina Del Vecchio Filipin, Miriam Paula Alonso Toldo, Luana Naiara Caetano, José Clóvis do Prado
It is well recognized that zinc is an essential trace element for all organisms, influencing growth and affecting the development and integrity of the immune system. It is also well known that the protective response against Trypanosoma cruzi depends on both innate and acquired immunity and for the control of the parasite load and host survival, the participation of special cells such natural killer (NK), T and B lymphocytes and macrophages are required. So the aims of this study were to evaluate the effects of zinc supplementation on the host's immune response infected with T...
June 14, 2008: Veterinary Parasitology
https://read.qxmd.com/read/18177642/trypanosoma-cruzi-the-effects-of-zinc-supplementation-during-experimental-infection
#26
JOURNAL ARTICLE
Vânia Brazão, Marina Del Vecchio Filipin, Leony Cristina Caetano, Miriam Paula Alonso Toldo, Luana Naiara Caetano, José Clóvis do Prado
It is well recognized that zinc is an essential trace element, influencing growth and affecting the development and integrity of the immune system. The use of oligoelements as zinc can be considered a tool in modulating the effectiveness of the immune response. In this work zinc was daily and orally supplied in male Wistar rats infected with the Y strain of Trypanosoma cruzi. Parasitemia was evaluated and a significant reduction on blood parasites was observed. In order to check some immunological parameters peritoneal macrophages were counted revealing higher percentages for zinc supplied group...
April 2008: Experimental Parasitology
https://read.qxmd.com/read/17996962/protein-geranylgeranyltransferase-i-of-trypanosoma-cruzi
#27
JOURNAL ARTICLE
Kohei Yokoyama, John R Gillespie, Wesley C Van Voorhis, Frederick S Buckner, Michael H Gelb
Protein geranylgeranyltransferase type I (PGGT-I) and protein farnesyltransferase (PFT) occur in many eukaryotic cells. Both consist of two subunits, the common alpha subunit and a distinct beta subunit. In the gene database of protozoa Trypanosoma cruzi, the causative agent of Chagas' disease, a putative protein that consists of 401 amino acids with approximately 20% amino acid sequence identity to the PGGT-I beta of other species was identified, cloned, and characterized. Multiple sequence alignments show that the T...
January 2008: Molecular and Biochemical Parasitology
https://read.qxmd.com/read/17827150/overexpression-of-a-zn2-sensitive-soluble-exopolyphosphatase-from-trypanosoma-cruzi-depletes-polyphosphate-and-affects-osmoregulation
#28
JOURNAL ARTICLE
Jianmin Fang, Felix A Ruiz, Melissa Docampo, Shuhong Luo, Juliany C F Rodrigues, Lucimar S Motta, Peter Rohloff, Roberto Docampo
We report the cloning, expression, purification, and characterization of the Trypanosoma cruzi exopolyphosphatase (TcPPX). The product of this gene (TcPPX), has 383 amino acids and a molecular mass of 43.1 kDa. TcPPX differs from most exopolyphosphatases in its preference for short-chain polyphosphate (poly P). Heterologous expression of TcPPX in Escherichia coli produced a functional enzyme that had a neutral optimum pH and was dramatically inhibited by low concentrations of Zn2+, high concentrations of basic amino acids (lysine and arginine), and heparin...
November 2, 2007: Journal of Biological Chemistry
https://read.qxmd.com/read/17188680/characterization-of-glycosomal-ring-finger-proteins-of-trypanosomatids
#29
JOURNAL ARTICLE
Tracy Saveria, Peter Kessler, Bryan C Jensen, Marilyn Parsons
The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Trypanosoma brucei, Leishmania major, and Trypanosoma cruzi. We expressed GFP fusions of each T. brucei coding region in procyclic form T. brucei, where they localized to glycosomes and behaved as integral membrane proteins...
May 2007: Experimental Parasitology
https://read.qxmd.com/read/17117371/tczfp8-a-novel-member-of-the-trypanosoma-cruzi-cchc-zinc-finger-protein-family-with-nuclear-localization
#30
JOURNAL ARTICLE
A O Ericsson, L O Faria, W B Cruz, C Martins de Sá, B D Lima
In a 17-kb genomic fragment of Trypanosoma cruzi chromosome XX, we identified three tandemly linked genes coding for CX(2)CX(4)HX(4)C zinc finger proteins. We also showed that similar genes are present in T. brucei and Leishmania major, sharing three monophyletic groups among these trypanosomatids. In T. cruzi, TcZFP8 corresponds to a novel gene coding for a protein containing eight zinc finger motifs. Molecular cloning of this gene and heterologous expression as a fusion with a His-tag were performed in Escherichia coli...
2006: Genetics and Molecular Research: GMR
https://read.qxmd.com/read/16958620/trypanothione-dependent-glyoxalase-i-in-trypanosoma-cruzi
#31
JOURNAL ARTICLE
Neil Greig, Susan Wyllie, Tim J Vickers, Alan H Fairlamb
The glyoxalase system, comprizing glyoxalase I and glyoxalase II, is a ubiquitous pathway that detoxifies highly reactive aldehydes, such as methylglyoxal, using glutathione as a cofactor. Recent studies of Leishmania major glyoxalase I and Trypanosoma brucei glyoxalase II have revealed a unique dependence upon the trypanosomatid thiol trypanothione as a cofactor. This difference suggests that the trypanothione-dependent glyoxalase system may be an attractive target for rational drug design against the trypanosomatid parasites...
December 1, 2006: Biochemical Journal
https://read.qxmd.com/read/16554554/a-tfiib-like-protein-is-indispensable-for-spliced-leader-rna-gene-transcription-in-trypanosoma-brucei
#32
JOURNAL ARTICLE
Bernd Schimanski, Jens Brandenburg, Tu Ngoc Nguyen, Melissa Jo Caimano, Arthur Günzl
The lack of general class II transcription factors was a hallmark of the genomic sequences of the human parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania major. However, the recent identification of TFIIA as part of a protein complex essential for RNA polymerase II-mediated transcription of SLRNA genes, which encode the trans splicing-specific spliced leader RNA, suggests that trypanosomatids assemble a highly divergent set of these factors at the SLRNA promoter. Here we report the identification of a trypanosomatid TFIIB-like (TFIIB(like)) protein which has limited overall sequence homology to eukaryotic TFIIB and archaeal TFB but harbors conserved residues within the N-terminal zinc ribbon domain, the B finger and cyclin repeat I...
2006: Nucleic Acids Research
https://read.qxmd.com/read/16442100/trypanosoma-rangeli-differential-expression-of-cell-surface-polypeptides-and-ecto-phosphatase-activity-in-short-and-long-epimastigote-forms
#33
JOURNAL ARTICLE
Suzete A O Gomes, André L Fonseca de Souza, Bianca A Silva, Tina Kiffer-Moreira, Jacenir R Santos-Mallet, André L S Santos, José R Meyer-Fernandes
Trypanosoma rangeli is a parasite of a numerous wild and domestic animals, presenting wide geographical distribution and high immunological cross-reactivity with Trypanosoma cruzi, which may lead to misdiagnosis. T. rangeli has a complex life cycle, involving distinct morphological and functional forms in the vector. Here, we characterized the cell surface polypeptides and the phosphatase activities in short and long epimastigotes forms of T. rangeli, using intact living parasites. The surface protein profile revealed by the incubation of parasites with biotin showed a preferential expression of the 97, 70, 50, 45, 25-22, and 15 kDa biotinylated polypeptides in the long forms, in contrast to the 55 and 28 kDa biotinylated polypeptides synthesized by the short epimastigotes...
April 2006: Experimental Parasitology
https://read.qxmd.com/read/16225937/tcpde4-a-novel-membrane-associated-camp-specific-phosphodiesterase-from-trypanosoma-cruzi
#34
JOURNAL ARTICLE
Guillermo D Alonso, Alejandra C Schoijet, Héctor N Torres, Mirtha M Flawiá
Cyclic nucleotide phosphodiesterases constitute the only known mechanism to inactivate regulatory signals involving cAMP or cGMP. In our laboratory a cAMP-specific phosphodiesterase associated to the flagellar apparatus, named TcPDE1, was identified in Trypanosoma cruzi. By using the catalytic domain sequence of TcPDE1 to screen a Trypanosoma cruzi genomic data base, a novel T. cruzi phosphodiesterase sequence was found and characterized. TcPDE4 encodes a 924-amino acid protein and shows homology with the PDE4 vertebrate subfamily...
January 2006: Molecular and Biochemical Parasitology
https://read.qxmd.com/read/15964555/protein-interactions-within-the-tczfp-zinc-finger-family-members-of-trypanosoma-cruzi-implications-for-their-functions
#35
JOURNAL ARTICLE
Florence Caro, Natalia Bercovich, Catalina Atorrasagasti, Mariano J Levin, Martín P Vázquez
The small zinc finger proteins tbZFP1 and tbZFP2 have been implicated in the control of Trypanosoma brucei differentiation to the procyclic form. Here, we report that the complete ZFP family in Trypanosoma cruzi is composed by four members, ZFP1A and B, and ZFP2A and B. ZFP1B is a paralog specific gene restricted to T. cruzi, while the ZFP2A and B paralogs diverged prior to the trypanosomatid lineage separation. Moreover, we demonstrate that TcZFP1 and TcZFP2 members interact with each other and that this interaction is mediated by a WW domain in TcZFP2...
August 5, 2005: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/12967710/trypanosoma-cruzi-poly-zinc-finger-protein-a-novel-dna-rna-binding-cchc-zinc-finger-protein
#36
JOURNAL ARTICLE
Joaquín M Espinosa, Daniel Portal, Guillermo S Lobo, Claudio A Pereira, Guillermo D Alonso, Eliana B Gómez, Graciela Humbert Lan, Rolando V Rivera Pomar, Mirtha M Flawiá, Héctor N Torres
A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins...
September 2003: Molecular and Biochemical Parasitology
https://read.qxmd.com/read/12209326/elemental-composition-of-acidocalcisomes-of-trypanosoma-cruzi-bloodstream-trypomastigote-forms
#37
JOURNAL ARTICLE
Alexandre Felip S Corrêa, Leonardo R Andrade, Maurilio J Soares
The elemental composition of acidocalcisomes present in Trypanosoma cruzi bloodstream trypomastigotes was analyzed by X-ray microanalysis. Acidocalcisomes appear as electron-dense vesicles dispersed throughout the cytoplasm. X-ray microanalysis of whole cells adhered to Formvar-coated grids showed that they contained C, O, Na, Mg, P, S, Ca and Zn, although in some acidocalcisomes iron was also detected. However, in ultra-thin sections of parasites processed according to routine procedures, most elements were lost...
October 2002: Parasitology Research
https://read.qxmd.com/read/10886860/-household-infestation-by-triatomines-and-human-seroprevalence-in-empedrado-department-corrientes-argentina
#38
Bar, Damborsky, Oscherov, Alvarez, Mizdraji, Avalos
Domestic and peridomestic triatomine colonization, T. infestans infection rate and human seropositives to Trypanosoma cruzi in rural areas of Empedrado Department, Corrientes, Argentina, were carried out. Methods used in order to search the triatomines, to determine them systematically, to obtain their infection rates and the human seropositives, were the same applied in a previous report. From 100 households characterized, 53.0% had mud walls, laminated zinc or cardboard combined with other elements were used in 37...
April 1997: Cadernos de Saúde Pública
https://read.qxmd.com/read/10722604/dual-role-for-transforming-growth-factor-beta-dependent-signaling-in-trypanosoma-cruzi-infection-of-mammalian-cells
#39
JOURNAL ARTICLE
B S Hall, M A Pereira
Expression of functional transforming growth factor beta (TGF-beta) receptors (TbetaR) is required for the invasion of mammalian cells by the protozoan parasite Trypanosoma cruzi. However, the precise role of this host cell signaling complex in T. cruzi infection is unknown. To investigate the role of the TGF-beta signaling pathway, infection levels were studied in the mink lung epithelial cell lines JD1, JM2, and JM3. These cells express inducible mutant TbetaR1 proteins that cannot induce growth arrest in response to TGF-beta but still transmit the signal for TGF-beta-dependent gene expression...
April 2000: Infection and Immunity
https://read.qxmd.com/read/9741092/tcdj1-a-putative-mitochondrial-dnaj-protein-in-trypanosoma-cruzi
#40
JOURNAL ARTICLE
M A Carreira, R S Tibbetts, C L Olson, C Schuster, M Renz, D M Engman, S Goldenberg
A full length cDNA encoding a novel Trypanosoma cruzi DnaJ protein was cloned and characterized. The 324 amino acid protein encoded by the cDNA (TcDJ1) displays a characteristics J-domain, but lacks the Gly-Phe and zinc finger regions present in some other DnaJ proteins. Relative to four other T. cruzi DnaJ proteins, TcDJ1 has an amino terminal extension containing basic and hydroxylated resides characteristic of mitochondrial import peptides. A T. cruzi transfectant expressing epitope-tagged TcDJ1 was generated and subcellular fractions were produced...
September 1, 1998: FEMS Microbiology Letters
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