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https://www.readbyqxmd.com/read/28817612/expression-and-functional-implications-of-the-renal-apelinergic-system-in-rodents
#1
Anne-Marie O'Carroll, Sabrine Salih, Philip R Griffiths, Aarifah Bijabhai, Mark A Knepper, Stephen J Lolait
Apelin binds to the G protein-coupled apelin receptor (APJ; gene name aplnr) to modulate diverse physiological systems including cardiovascular function, and hydromineral and metabolic balance. Recently a second endogenous ligand for APJ, named apela, has been discovered. We confirm that apela activates signal transduction pathways (ERK activation) in cells expressing the cloned rat APJ. Previous studies suggest that exogenous apela is diuretic, attributable wholly or in part to an action on renal APJ. Thus far the cellular distribution of apela in the kidney has not been reported...
2017: PloS One
https://www.readbyqxmd.com/read/28815590/juxtanodin-in-retinal-pigment-epithelial-cells-expression-and-biological-activities-in-regulating-cell-morphology-and-actin-cytoskeleton-organization
#2
Fengyi Liang, Ji Hyun Hwang, Nicholas Weiwei Tang, Walter Hunziker
Juxtanodin (also known as ermin) was initially identified as an actin cytoskeleton-related oligodendroglial protein in the rat central nervous system. It was subsequently also found in the rat olfactory neuroepithelium, especially at the apical junctional belt of the sustentacular cells. We further examined juxtanodin expression and functional roles in the retina using fluorescence histochemistry, confocal microscopy, immuno-electron microscopy, molecular biology and cell culture. Prominent juxtanodin expression was found in the photoreceptor-supporting retinal pigment epithelium (RPE), especially in a zone corresponding to the apices of RPE cells, at the roots of the RPE microvilli, and at the base of RPE cells next to the Bruch's membrane...
August 17, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28812533/immunoreactivity-of-canine-liposarcoma-to-muscle-and-brown-adipose-antigens
#3
Elise Eva Bella LaDouceur, Sarah E Stevens, Jason Wood, Christopher M Reilly
Liposarcoma, rhabdomyosarcoma, and hibernoma share some overlapping histologic and immunohistochemical features. Although immunohistochemistry (IHC) is commonly used in the diagnosis of these neoplasms, expression of muscle markers has been reported in human liposarcoma and canine hibernoma in addition to rhabdomyosarcoma. Thus, these neoplasms are a diagnostic challenge but important to distinguish because of differences in prognosis and treatment. Rhabdomyosarcoma and liposarcoma are both malignant, but rhabdomyosarcoma has a higher potential for metastasis...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28811140/divergent-effects-of-resistance-training-and-anabolic-steroid-on-the-postsynaptic-region-of-different-skeletal-muscles-of-aged-rats
#4
Walter Krause Neto, Wellington de Assis Silva, Adriano Polican Ciena, Carlos Alberto Anaruma, Eliane Florencio Gama
This study aimed to analyze the effects of resistance training associated with testosterone administration in the neuromuscular junction (NMJ) postsynaptic region of different skeletal muscle types of aged rats. Wistar rats were divided into: SEI - 20-months-old control, SEF - 24-months-old control, T - 20-months-old with testosterone, S - 20-months-old resistance trained and ST - 20-months-old with resistance training associated with testosterone propionate. All groups were submitted to familiarization and maximum load carrying testing (MLCT)...
August 12, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28810596/expression-of-kruppel-like-factor-8-and-ki67-in-lung-adenocarcinoma-and-prognosis
#5
Yifei Liu, Xiufang Yao, Qing Zhang, Li Qian, Jia Feng, Tingting Bian, Jianguo Zhang, Ye Tian
Kruppel-like factor 8 (KLF8) belongs to the KLF family and has various roles in the regulation of the cell cycle, proliferation and tumor genesis. KLF8 is overexpressed in gastric, ovarian, breast and renal cancer. Additionally, KLF8 may affect invasion and metastasis of tumors. However, whether KLF8 also acts as an ontogeny in lung adenocarcinoma (LAC) remains unknown. The aim of the present study was to determine the association between KLF8 expression and various clinical and pathological parameters. Western blot assays and immune histochemistry analyses revealed that KLF8 level in LAC tissues was higher than that in the normal lung tissues and KLF8 expression was significantly associated with clinical variables (P<0...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28810187/histochemical-and-immunohistochemical-examination-of-odontoblasts-petroblasts-in-petrodentine-formation-of-lungfish
#6
Shunya Oka, Ichiro Sasagawa, Mikio Ishiyama
OBJECTIVE: Petrodentine, the core of the lungfish tooth plate, is a well-mineralized tissue similar to mammalian enamel and analogous to enameloid in fish teeth. Petrodentine is formed solely by petroblasts, which are specialized odontoblasts, whereas enameloid is a composite tissue produced by both odontoblasts and dental epithelial cells. To clarify the details of petrodentine formation, petroblasts were investigated using histochemical and immunohistochemical techniques. METHODS: Extant lungfish (Lepidosiren paradoxa) were used in this study...
August 3, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28800329/chronic-verubecestat-treatment-suppresses-amyloid-accumulation-in-advanced-aged-tg2576-a%C3%AE-ppswe-mice-without-inducing-microhemorrhage
#7
Stephanie Villarreal, Fuqiang Zhao, Lynn A Hyde, Daniel Holder, Thomas Forest, Marie Sondey, Xia Chen, Cyrille Sur, Eric M Parker, Matthew E Kennedy
Verubecestat is a potent BACE1 enzyme inhibitor currently being investigated in Phase III trials for the treatment of mild-to-moderate and prodromal Alzheimer's disease. Multiple anti-amyloid immunotherapies have been dose-limited by adverse amyloid related imaging abnormalities such as vasogenic edema (ARIA-E) and microhemorrhage (ARIA-H) observed in human trials and mice. Verubecestat was tested in a 12-week nonclinical study for the potential to exacerbate microhemorrhage (ARIA-H) profiles in 18-22-month-old post-plaque Tg2576-AβPPswe mice...
2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28799534/cutaneous-plasmacytosis-a-rare-entity-with-unique-presentation
#8
Subhra Dhar, Lalthleng Liani, Kamlakar Patole, Sandipan Dhar
Primary cutaneous plasmacytosis is a rare cutaneous disorder with extensive cutaneous plaques/papules mainly on the trunk and face. Cases have mostly been documented from Japan. We present here a rare case of cutaneous plasmacytosis from India of Mongolian descent. This 50-year-old female from Mizoram had extensive maculo-papular violaceous plaques distributed on the face, axillae, trunk and lower extremities. Initial and repeat skin biopsy revealed dense perivascular and periadnexal mature plasma cells. She also had lymphadenopathy...
August 9, 2017: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/28790886/molecular-bases-of-brain-preconditioning
#9
Oleg G Deryagin, Svetlana A Gavrilova, Khalil L Gainutdinov, Anna V Golubeva, Vyatcheslav V Andrianov, Guzel G Yafarova, Sergey V Buravkov, Vladimir B Koshelev
Preconditioning of the brain induces tolerance to the damaging effects of ischemia and prevents cell death in ischemic penumbra. The development of this phenomenon is mediated by mitochondrial adenosine triphosphate-sensitive potassium ([Formula: see text]) channels and nitric oxide signaling (NO). The aim of this study was to investigate the dynamics of molecular changes in mitochondria after ischemic preconditioning (IP) and the effect of pharmacological preconditioning (PhP) with the [Formula: see text]-channels opener diazoxide on NO levels after ischemic stroke in rats...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28778073/expression-of-ezh2-in-uveal-melanomas-patients-and-associations-with-prognosis
#10
Ying Cheng, Ying Li, Xin Huang, Wenbin Wei, Yi Qu
PURPOSE: To analyze the prognostic value and potential target for therapeutic intervention of enhancer of zeste homologue 2 (EZH2) in uveal melanomas (UM) patients. METHOD: We analyzed EZH2 expression in 89 primary UM patients by immuno- histochemistry to observe the clinicopathological and prognostic value of EZH2. RESULTS: The high levels of mitoses count and Ki67 labeling index had significant correlation with overexpression EZH2 (R = 0...
July 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28776458/sufentanil-medetomidine-anaesthesia-compared-with-fentanyl-fluanisone-midazolam-is-associated-with-fewer-ventricular-arrhythmias-and-death-during-experimental-myocardial-infarction-in-rats-and-limits-infarct-size-following-reperfusion
#11
Ellis N Ter Horst, Paul A J Krijnen, Paul Flecknell, Klaas W Meyer, Klaas Kramer, Anja M van der Laan, Jan J Piek, Hans W M Niessen
To improve infarct healing following myocardial infarction in humans, therapeutic interventions can be applied during the inflammatory response. Animal models are widely used to study this process. However, induction of MI in rodents is associated with high mortality due to ventricular fibrillation (VF) during coronary artery ligation. The anaesthetic agent used during the procedure appears to influence the frequency of this complication. In this retrospective study, the effect on ventricular arrhythmia incidence during ligation and infarct size following in vivo reperfusion of two anaesthetic regimens, sufentanil-medetomidine (SM) and fentanyl/fluanisone-midazolam (FFM) was evaluated in rats...
January 1, 2017: Laboratory Animals
https://www.readbyqxmd.com/read/28771840/influence-of-l-carnitine-and-l-arginine-on-protein-synthesis-and-maturation-of-the-semitendinosus-muscle-of-lightweight-piglets
#12
J G Madsen, E Seoni, M Kreuzer, P Silacci, G Bee
Lightweight (LW) piglets from large litters display impaired growth performance compared with heavier littermates. This study investigated the growth performance and muscle development of early-weaned LW piglets (birthweight <1.2 kg) from large litters (17.3 ± 3.0 total born per litter), fed ad libitum a milk replacer supplemented with either l-carnitine (CAR) or l-arginine (ARG) from day 7 to day 28 of age. In total, 36 female and entire male Swiss Large White piglets, weaned on day 7 of age, were artificially reared in pairs in rescue decks...
August 3, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28770679/the-stomatogastric-and-enteric-nervous-system-of-the-pulmonate-snail-megalobulimus-abbreviatus-a-neurochemical-analysis
#13
Malcon Andrei Martinez-Pereira, Raphaela da Cunha Franceschi, Bárbara Paranhos Coelho, Denise M Zancan
Chemical coding of stomatogastric nervous system (STNS) and enteric nervous system (ENS) of midgut and hindgut in the snail Megalobulimus abbreviatus was investigated using histochemistry, histofluorescence, and immunohistochemistry. The gastrointestinal plexuses, constituted by intrinsic neurons and fibers originating from the subesophageal ganglia and/or STNS, showed intense acetylcholinesterase (AChE) and nicotinamide adenine dinucleotide diaphorase (NADPHd) activity. The enteric neurons and fibers with AChE activity are scattered in the submucosa and between both muscular layers of gastrointestinal tract, whereas NADPHd neurons and fibers are more abundant between muscular layers than in the submucosa...
August 2017: Zoological Science
https://www.readbyqxmd.com/read/28755996/receptor-visualization-and-the-atomic-bomb-a-historical-account-of-the-development-of-the-chemical-neuroanatomy-of-receptors-for-neurotransmitters-and-drugs-during-the-cold-war
#14
REVIEW
J M Palacios, G Mengod
This is a historical account of how receptors for neurotransmitters and drugs got to be seen at the regional, cellular, and subcellular levels in brain, in the years going from the end of the World War II until the collapse of the Soviet Union, the Cold War (1945-1991). The realization in the US of the problem of mental health care, as a consequence of the results of medical evaluation for military service during the war, let the US Government to act creating among other things the National Institute for Mental Health (NIMH)...
July 26, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28752591/comparison-and-evaluation-of-capacitation-and-acrosomal-reaction-in-freeze-thawed-human-ejaculated-spermatozoa-treated-with-l-carnitine-and-pentoxifylline
#15
E Aliabadi, S Jahanshahi, T Talaei-Khozani, M Banaei
Cryopreservation is used to preserve the spermatozoa; however, it leads to a reduction in sperm quality. L-carnitine (LC) influences sperm motility and preserves the sperm membrane and DNA integrity. The objectives of this study were to evaluate the protective effects of LC on the membrane integrity of normal human spermatozoa and compare it with pentoxifylline (PT) during cryopreservation. Thirty normal semen samples, prepared by swim-up procedure, were divided into three aliquots: a control without any treatment and two experimental aliquots that were incubated in PT or LC for 30 min...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28751290/myoadenylate-deaminase-deficiency-a-frequent-cause-of-muscle-pain-a-case-detected-by-exercise-testing
#16
Lydie Lim, Maeva Palayer, Antoine Bruneau, Franck Letournel, Cédric Le Maréchal, Gilles Simard, Pascal Reynier, Chadi Homedan, Aleksandra Nadaj-Pakleza
Myoadenylate deaminase deficit (MAD, MIM#615511) is the most common cause of metabolic myopathies with an estimated prevalence of 1-2% in the general population. We report the case of a 39-year-old man suffering from severe skeletal muscle pain that had developed gradually for 4 years. A moderate increase in creatine kinase (CK) was the only biological sign observed. This study takes a closer look at a common but poorly known pathology and highlights the interest of the dynamic metabolic investigations carried out during exercise stress test with a cycle ergometer...
August 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28748686/invisible-dermatoses-from-the-perspective-of-the-dermatopathologist-original-observations-and-novel-concepts
#17
Carlo Tomasini
The phrase 'invisible dermatoses' has been used in two different contexts: 1) Invisible dermatoses to the clinician, i.e., diseases with no significant clinical features, 2) invisible dermatoses to the pathologist. These latter comprise a wide spectrum of entities which present with definite and obvious clinical features, but subtle or hidden histological features and are therefore "invisible" histologically. Diagnosis of such diseases represents a great challenge to both the dermatologist and dermatopathologist...
July 26, 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28748640/expression-of-nad-p-h-quinone-dehydrogenase-1-nqo1-is-increased-in-the-endometrium-of-women-with-endometrial-cancer-and-women-with-polycystic-ovary-syndrome
#18
William Atiomo, Mohamad Nasir Shafiee, Caroline Chapman, Veronika M Metzler, Jad Abouzeid, Ayşe Latif, Amy Chadwick, Sarah Kitson, Vanitha N Sivalingam, Ian J Stratford, Catrin S Rutland, Jenny L Persson, Niels Ødum, Pablo Fuentes-Utrillia, Jennie N Jeyapalan, David M Heery, Emma J Crosbie, Nigel P Mongan
OBJECTIVE: Women with a prior history of polycystic ovary syndrome (PCOS) have an increased risk of endometrial cancer (EC). AIM: To investigate whether the endometrium of women with PCOS possess gene expression changes similar to those found in EC. DESIGN AND METHODS: Patients with EC, PCOS and control women unaffected by either PCOS or EC were recruited into a cross-sectional study at the Nottingham University Hospital, UK. For RNA sequencing, representative individual endometrial biopsies were obtained from women with EC, PCOS and a woman unaffected by PCOS or EC...
July 27, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28735523/histochemistry-and-immunolocalisation-of-glucokinin-in-antidiabetic-plants-used-in-traditional-mexican-medicine
#19
Guillermo Laguna-Hernández, Carlos A Rio-Zamorano, Itzel G Meneses-Ochoa, Alicia E Brechú-Franco
Mexico is a megadiverse country that has 3,600 to 4,000 species of medicinal plants, of which approximately 800 are used to treat conditions related to diabetes mellitus (DM). DM is a chronic degenerative disease of energy metabolism that exists as two types: type 1 (DM1) and type 2 (DM2). DM is considered a public health problem that affects 7% of the Mexican population older than 20 years. DM is clinically controlled with hypoglycaemic drugs, alpha-glucosidase inhibitors, insulin secretion stimulants or the direct application of insulin...
June 21, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28730689/relationships-among-muscle-fiber-type-composition-fiber-diameter-and-mrf-gene-expression-in-different-skeletal-muscles-of-naturally-grazing-wuzhumuqin-sheep-during-postnatal-development
#20
Qimuge Siqin, Tadayuki Nishiumi, Takahisa Yamada, Shuiqing Wang, Wenjun Liu, Rihan Wu, Gerelt Borjigin
The aim of this study was to determine the relationships among muscle fiber-type composition, fiber diameter, and myogenic regulatory factor (MRF) gene expression in different skeletal muscles during development in naturally grazing Wuzhumuqin sheep. Three major muscles (i.e. the Longissimus dorsi (LD), Biceps femoris (BF) and Triceps brachii (TB)) were obtained from 20 Wuzhumuqin sheep and 20 castrated rams at each of the following ages: 1, 3, 6, 9, 12 and 18 months. Muscle fiber-type composition and fiber diameter were measured using histochemistry and morphological analysis, and MRF gene expression levels were determined using real-time PCR...
July 20, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
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