Dietmar Kültz, Alison M Gardell, Anthony DeTomaso, Greg Stoney, Baruch Rinkevich, Yuval Rinkevich, Andy Qarri, Weizhen Dong, Brenda Luu, Mandy Lin
Botryllus schlosseri, is a model marine invertebrate for studying immunity, regeneration, and stress-induced evolution. Conditions for validating its predicted proteome were optimized using nanoElute® 2 deep-coverage LCMS, revealing up to 4930 protein groups and 20,984 unique peptides per sample. Spectral libraries were generated and filtered to remove interferences, low-quality transitions, and only retain proteins with >3 unique peptides. The resulting DIA assay library enabled label-free quantitation of 3426 protein groups represented by 22,593 unique peptides...
February 24, 2024: Proteomics