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https://www.readbyqxmd.com/read/28445069/targeted-dereplication-of-microbial-natural-products-by-high-resolution-ms-and-predicted-lc-retention-time
#1
Justine Chervin, Marc Stierhof, Ming Him Tong, Doe Peace, Kine Østnes Hansen, Dagmar Solveig Urgast, Jeanette Hammer Andersen, Yi Yu, Rainer Ebel, Kwaku Kyeremeh, Veronica Paget, Gabriela Cimpan, Albert Van Wyk, Hai Deng, Marcel Jaspars, Jioji N Tabudravu
A new strategy for the identification of known compounds in Streptomyces extracts that can be applied in the discovery of natural products is presented. The strategy incorporates screening a database of 5555 natural products including 5098 structures from Streptomyces sp., using a high-throughput LCMS data processing algorithm that utilizes HRMS data and predicted LC retention times (tR) as filters for rapid identification of known compounds in the natural product extract. The database, named StrepDB, contains for each compound the structure, molecular formula, molecular mass, and predicted LC retention time...
April 26, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28441352/high-throughput-determination-of-sodium-danshensu-in-beagle-dogs-by-the-lcms-ms-method-employing-liquid-liquid-extraction-based-on-96-well-format-plates
#2
Jingjing Jiang, Xin Zhao, Xiuxiu Li, Shengyuan Wu, Shidan Yu, Yuefen Lou, Guorong Fan
Sodium Danshensu (sodium d-(+)-β-(3,4-dihydroxyphenyl) lactate), one of the water-soluble ingredients in Salvia miltiorrhiza, exhibits potent relaxation of the coronary artery and anticoagulation effection. A high-throughput, rapid, and sensitive method combining liquid chromatography with electrospray ionization tandem mass spectrometry to determine the sodium danshensu in beagle dog plasma was developed and validated, using gallic acid as an internal standard (IS). Acidified plasma samples were extracted using 96-well liquid-liquid extraction, and were eluted on a CNW Athena C18 column (3 μm, 2...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28433718/three-new-piscidins-from-orange-spotted-grouper-epinephelus-coioides-phylogeny-expression-and-functional-characterization
#3
Ze-Rui Zhuang, Xiao-Dong Yang, Xia-Zi Huang, Hui-Xian Gu, Han-Yin Wei, Yi-Jun He, Li Deng
The present study reports the identification, and characterization of three new putative piscidin paralogues, ecPis-2, ecPis-3 and ecPis-4, from orange-spotted grouper (Epinephelus coioides). The cDNA of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. The phylogenetic analysis indicated that multiple piscidin paralogues in one fish species are highly diversified, the analysis suggested that the piscidins should be a family belonging to the superfamily of ancient cationic, linear, and amphipathic host defence peptides widespread across invertebrate and vertebrate taxa comprising insect cecropins and ceratotoxins, and the amphibian dermaseptins...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28424496/the-chemical-characterization-of-nigerian-propolis-samples-and-their-activity-against-trypanosoma-brucei
#4
Ruwida Omar, John O Igoli, Tong Zhang, Alexander I Gray, Godwin U Ebiloma, Carol J Clements, James Fearnley, RuAngeli Edrada Ebel, Tim Paget, Harry P de Koning, David G Watson
Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28401460/enhanced-production-of-berberine-in-in-vitro-regenerated-cell-of-tinospora-cordifolia-and-its-analysis-through-lcms-qtof
#5
Jitendra Mittal, Madan Mohan Sharma
Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of multiple shoot formation was induced when the nodal segments were cultured on MS medium supplemented with BAP (1.0 mg L(-1)) and 2-iP (0.5 mg L(-1)). The highest mean number of shoots per nodal explant (7...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28392192/ester-and-amide-containing-multiqacs-exploring-multicationic-soft-antimicrobial-agents
#6
Ryan A Allen, Megan C Jennings, Myles A Mitchell, Saleh E Al-Khalifa, William M Wuest, Kevin P C Minbiole
Quaternary ammonium compounds (QACs) are ubiquitous antiseptics whose chemical stability is both an aid to prolonged antibacterial activity and a liability to the environment. Soft antimicrobials, such as QACs designed to decompose in relatively short times, show the promise to kill bacteria effectively but not leave a lasting footprint. We have designed and prepared 40 soft QAC compounds based on both ester and amide linkages, in a systematic study of mono-, bis-, and tris-cationic QAC species. Antimicrobial activity, red blood cell lysis, and chemical stability were assessed...
March 29, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28391542/an-hbt-based-near-infrared-fluorescent-probe-for-colorimetric-and-ratiometric-detection-of-bisulfite-and-its-application-in-living-cells
#7
Yuanchao Sun, Zhen Chen, Fengzao Chen, Heng Liu, Hanping He, Xiuhua Zhang, Shengfu Wang
In this paper, a new type of ratiometric fluorescent probe HBT-TCF with large emission shift (up to 200 nm) has been developed for sensing HSO3(-) based on Michael-type addition reaction. The probe could fast recognize of HSO3(-) within 3 min in PBS buffer solution. The detection limit of the probe for HSO3(-) is as low as 101 nM. Meanwhile, the sensing mechanism is confirmed by NMR, LCMS. In addition, the probe can be applied to detect HSO3(-) in living cells.
April 9, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28374316/modulation-of-protein-fragmentation-through-carbamylation-of-primary-amines
#8
Sylvester M Greer, Dustin D Holden, Ryan Fellers, Neil L Kelleher, Jennifer S Brodbelt
We evaluate the impact of carbamylation of the primary amines of the side-chains of Lys and the N-termini on the fragmentation of intact protein ions and the chromatographic properties of a mixture of E. coli ribosomal proteins. The fragmentation patterns of the six unmodified and carbamylated proteins obtained by higher energy collision dissociation (HCD) and ultraviolet photodissociation (UVPD) were compared. Carbamylation significantly reduced the total number of protons retained by the protein owing to the conversion of basic primary amines to non-basic carbamates...
April 3, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28355591/effect-of-aquo-alchoholic-extract-of-glycyrrhiza-glabra-against-pseudomonas-aeruginosa-in-mice-lung-infection-model
#9
Ankita Singh Chakotiya, Ankit Tanwar, Pranay Srivastava, Alka Narula, Rakesh Kumar Sharma
The prevalence of lung infection caused by Pseudomonas aeruginosa strains that are classified as multi-drug resistant has increased considerably and is mainly attributed to relative insufficiency of potent chemotherapeutic modalities. The present study was conducted to evaluate the antimicrobial activity of aquo-alcoholic extract of Glycyrrhiza glabra against the P. aeruginosa causing lung infection in Swiss albino mice. The study involves evaluation of lethal dose of P. aeruginosa in Swiss albino mice and analysis of disease manifestation that includes bacteremia, hypothermia, reduction in body weight and other parameters for 48h of infection...
March 26, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28347309/chemical-composition-and-pharmacological-activities-of-pisum-sativum
#10
Md Nazmul Hasan Zilani, Tamanna Sultana, S M Asabur Rahman, Md Anisuzzman, Md Amirul Islam, Jamil A Shilpi, Md Golam Hossain
BACKGROUND: Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out. METHODS: Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu's reagent while the flavonoids by aluminum chloride colorimetric assay...
March 27, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28334552/isolation-and-characterization-of-novel-degradation-products-of-doxofylline-using-hplc-ftir-lcms-and-nmr
#11
Ch Krishnam Raju, Avadhesh K Pandey, Gururaj S, Kaushik Ghosh, Arunima Pola, Sanath Kumar Goud P, Mrunal A Jaywant, Sameer G Navalgund
Forced degradation of Doxofylline (DFL) in different stress (base and peroxide) conditions gave rise to two potential unknown impurities. These unknown degradation products DFL DEG-I and DFL DEG-II were evaluated using a new-reverse-phase high performance liquid chromatography (HPLC), where it was eluted at 0.44 and 1.09 relative retention times to DFL peak. DFL DEG-I and DFL DEG-II were isolated using preparative HPLC from degradation mixtures. The structure of DFL DEG-I and DFL DEG-II were elucidated using high resolution MS, multi-dimensional NMR and FTIR spectroscopic techniques, and characterized...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28330318/brevibacillus-borstelensis-and-streptomyces-albogriseolus-have-roles-to-play-in-degradation-of-herbicide-sulfosulfuron
#12
Ridhima Arya, Navnit Kumar Mishra, Anil K Sharma
Use of herbicides, in particular sulfosulfuron, at more than recommended doses has raised major concerns about the health hazards for animals and humans. In the present study, isolation of sulfosulfuron-degrading Brevibacillus borstelensis and Streptomyces albogriseolus from the field soils in the northwestern region of India was carried out where the use of sulfosulfuron is predominant, and further assessed for their potential to degrade sulfosulfuron individually and together in a consortium form under lab conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330214/production-kinetics-of-polyhydroxyalkanoates-by-using-pseudomonas-aeruginosa-gamma-ray-mutant-strain-ebn-8-cultured-on-soybean-oil
#13
Sharjeel Abid, Zulfiqar Ali Raza, Tanveer Hussain
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330200/use-of-ca-alginate-immobilized-pseudomonas-aeruginosa-for-repeated-batch-and-continuous-degradation-of-endosulfan
#14
Vijayalakshmi Pradeep, Usha Malavalli Subbaiah
The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was carried out with an objective of reducing the toxicity of Endosulfan by degrading it to less toxic metabolites. The long-term stability of Endosulfan degradation was studied during its repeated batch degradation, carried out over a period of 35 days...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28321795/phytase-production-by-aspergillus-niger-ncim-563-for-a-novel-application-to-degrade-organophosphorus-pesticides
#15
Parin C Shah, V Ravi Kumar, Syed G Dastager, Jayant M Khire
The production of phytase using Aspergillus niger NCIM 563 under submerged fermentation conditions was studied using protein rich chickpea flour as substrate. Employing a hybrid statistical media optimization strategy of Plackett-Burman and Box-Behnken experimental designs in shake-flasks gave an increased phytase activity from an initial 66 IU/mL in 216 h to 160 IU/mL in a reduced time of 132 h. Productivity, thus increased by 3.97 times from 7.3 to 29 IU/mL/day. Using the optimized media, the production was successfully scaled-up further and improved up to 164 IU/mL in 96 h by studies carried out employing 2 and 10-L fermenters...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28321210/production-of-ramoplanin-and-ramoplanin-analogs-by-actinomycetes
#16
Mercedes de la Cruz, Ignacio González, Craig A Parish, Russell Onishi, José R Tormo, Jesús Martín, Fernando Peláez, Debbie Zink, Noureddine El Aouad, Fernando Reyes, Olga Genilloud, Francisca Vicente
Ramoplanin is a glycolipodepsipeptide antibiotic obtained from fermentation of Actinoplanes sp. ATCC 33076 that exhibits activity against clinically important multi-drug-resistant, Gram-positive pathogens including vancomycin-resistant Enterococcus (VRE), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-intermediate resistant Clostridium difficile. It disrupts bacterial cell wall through a unique mechanism of action by sequestering the peptidoglycan intermediate Lipid II and therefore does not show cross-resistance with other antibiotics...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28317412/bioactivity-guided-isolation-of-cytotoxic-terpenoids-and-steroids-from-premna-serratifolia
#17
Mahesh Biradi, Kirankumar Hullatti
CONTEXT: Despite several phytochemical studies of Premna serratifolia Linn. (Verbenaceae), the isolation of active constituents of this plant remains to be explored. OBJECTIVE: The study isolates cytotoxic terpenoids and steroids from the leaves of Premna serratifolia. MATERIALS AND METHODS: Unsaponifiable matter of hexane soluble fraction obtained from methanol extract was subjected to isolation by column chromatography and preparative TLC...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28298353/simultaneous-assay-of-cortisol-and-dexamethasone-improved-diagnostic-accuracy-of-the-dexamethasone-suppression-test
#18
Grethe Å Ueland, Paal Methlie, Ralf Kellmann, Marit Bjørgaas, Bjørn O Åsvold, Ketil Thorstensen, Oskar Kelp, Hrafnkell B Thordarson, Gunnar Mellgren, Kristian Løvås, Eystein S Husebye
OBJECTIVES: The overnight dexamethasone (DXM) suppression test (DST) has high sensitivity, but moderate specificity, for diagnosing hypercortisolism. We have evaluated if simultaneous measurement of S-DXM may correct for variable DXM bioavailability and increase the diagnostic performance of DST, and if saliva (sa) is a feasible adjunct or alternative to serum. DESIGN AND METHODS: Prospective study of DST was carried out in patients with suspected Cushing's syndrome (CS) (n = 49), incidentaloma (n = 152) and healthy controls (n = 101)...
June 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28286460/lipid-response-patterns-in-acute-phase-paediatric-plasmodium-falciparum-malaria
#19
Judy Orikiiriza, Izabella Surowiec, Elisabeth Lindquist, Mari Bonde, Jimmy Magambo, Charles Muhinda, Sven Bergström, Johan Trygg, Johan Normark
INTRODUCTION: Several studies have observed serum lipid changes during malaria infection in humans. All of them were focused at analysis of lipoproteins, not specific lipid molecules. The aim of our study was to identify novel patterns of lipid species in malaria infected patients using lipidomics profiling, to enhance diagnosis of malaria and to evaluate biochemical pathways activated during parasite infection. METHODS: Using a multivariate characterization approach, 60 samples were representatively selected, 20 from each category (mild, severe and controls) of the 690 study participants between age of 0...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28281887/rapid-assessment-of-oxidation-via-middle-down-lcms-correlates-with-methionine-side-chain-solvent-accessible-surface-area-for-121-clinical-stage-monoclonal-antibodies
#20
Rong Yang, Tushar Jain, Heather Lynaugh, R Paul Nobrega, Xiaojun Lu, Todd Boland, Irina Burnina, Tingwan Sun, Isabelle Caffry, Michael Brown, Xiaoyong Zhi, Asparouh Lilov, Yingda Xu
Susceptibility of methionine to oxidation is an important concern for chemical stability during the development of a monoclonal antibody (mAb) therapeutic. To minimize downstream risks, leading candidates are usually screened under forced oxidation conditions to identify oxidation-labile molecules. Here we report results of forced oxidation on a large set of in-house expressed and purified mAbs with variable region sequences corresponding to 121 clinical stage mAbs. These mAb samples were treated with 0.1% H2O2 for 24 hours before enzymatic cleavage below the hinge, followed by reduction of inter-chain disulfide bonds for the detection of the light chain, Fab portion of heavy chain (Fd) and Fc by liquid chromatography-mass spectrometry...
May 2017: MAbs
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