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https://www.readbyqxmd.com/read/29673441/ywhaz-binds-to-trim21-but-is-not-involved-in-trim21-stimulated-osteosarcoma-cell-proliferation
#1
Qing Zhong Zeng, Wan Ting Liu, Jun Lei Lu, Xiao Hui Liu, Yun Fang Zhang, Lang Xia Liu, Xue Juan Gao
OBJECTIVE: Osteosarcoma is the most common type of malignant bone tumor in children and adolescents. The role of E3 ligases in tumorigenesis is currently a focus in tumor research. In the present study, we investigated the role of the E3 ligase tripartite motif 21 (TRIM21) in osteosarcoma cell proliferation. METHODS: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays were used to assess osteosarcoma cell viability. U2-OS cells stably carrying a recombinant lentivirus expressing tetracycline-regulated TRIM21 were screened...
March 2018: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/29663150/preparation-of-a-novel-lipid-core-micelle-using-a-low-energy-emulsification-method
#2
Hans F Fritz, Andrea C Ortiz, Sitaram P Velaga, Javier O Morales
High-energy methods for the manufacturing of nanomedicines are widely used; however, interest in low-energy methods is increasing due to their simplicity, better control over the process, and energy-saving characteristics during upscaling. Here, we developed a novel lipid-core micelle (LCM) as a nanocarrier to encapsulate a poorly water-soluble drug, nifedipine (NFD), by hot-melt emulsification, a low-energy method. LCMs are self-assembling colloidal particles composed of a hydrophobic core and a hydrophilic shell...
April 16, 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29648453/liquid-crystal-monomers-lcms-a-new-generation-of-persistent-bioaccumulative-and-toxic-pbt-compounds
#3
Jianhua Li, Guanyong Su, Robert J Letcher, Wanqing Xu, Mengyun Yang, Yayun Zhang
No abstract text is available yet for this article.
April 12, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29643307/quantification-of-trans-resveratrol-in-red-wines-using-quechers-extraction-combined-with-liquid-chromatography-tandem-mass-spectrometry
#4
Shih-Chieh Lu, Wan-Rou Liao, Sung-Fang Chen
Resveratrol is one of representative ingredient in red wine, but its quantification is a challenge because of a complex and abundant matrix. In this study, two sample pretreatments, direct dilution and QuEChERS extraction, coupling LCMS analysis were examined for resveratrol quantification. Similar recoveries of 106.4 to 93.7% were obtained for direct dilution and QuEChERS, respectively. With the aid of condition optimization, QuEChERS extraction could concentrate the resveratrol from red wines to improve the detection sensitivity with a LOD value of 2...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29626642/haemostatic-effects-of-latex-from-croton-sparsiflorus-morang-in-vitro-in-vivo-in-silico-approaches
#5
M C Kamaraj, S Mohan Raj, D Palani Selvam, S Subashchandrabose, A Kalaiselvan
The present investigations are phytochemical screening of Latex aqueous (Laq ) extract of C. sparsiflorus and study its role in homeostasis. It is being traditionally used for fresh cuts to stop bleeding immediately. To know the contents of extract, the quantitative phytochemical analysis were performed it showed the contents such as saponins (15.2%), alkaloids (7.61%), phenols (0.62%), tannins (1.1%), and flavonoids (0.224%). The in vitro and in vivo blood clotting mechanism was observed in Wister albino rats to understand the blood clotting activity...
March 27, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29580617/biocontrol-activity-of-surfactin-a-purified-from-bacillus-nh-100-and-nh-217-against-rice-bakanae-disease
#6
Ambrin Sarwar, Muhammad Nadeem Hassan, Muhammad Imran, Mazhar Iqbal, Saima Majeed, Günter Brader, Angela Sessitsch, Fauzia Yusuf Hafeez
The potential of the Bacillus genus to antagonize phytopathogens is associated with the production of cyclic lipopeptides. Depending upon the type of lipopeptide, they may serve as biocontrol agents that are eco-friendly alternatives to chemical fertilizers. This study evaluates the biocontrol activity of surfactin-producing Bacillus (SPB) strains NH-100 and NH-217 and purified surfactin A from these strains against rice bakanae disease. Biologically active surfactin fractions were purified by HPLC, and surfactin A variants with chain lengths from C12 to C16 were confirmed by LCMS-ESI...
April 2018: Microbiological Research
https://www.readbyqxmd.com/read/29574346/evaluation-of-three-rapid-oral-fluid-test-devices-on-the-screening-of-multiple-drugs-of-abuse-including-ketamine
#7
Magdalene H Y Tang, C K Ching, Simon Poon, Suzanne S S Chan, W Y Ng, M Lam, C K Wong, Ronnie Pao, Angus Lau, Tony W L Mak
Rapid oral fluid testing (ROFT) devices have been extensively evaluated for their ability to detect common drugs of abuse; however, the performance of such devices on simultaneous screening for ketamine has been scarcely investigated. The present study evaluated three ROFT devices (DrugWipe® 6S, Ora-Check® and SalivaScreen® ) on the detection of ketamine, opiates, methamphetamine, cannabis, cocaine and MDMA. A liquid chromatography tandem mass spectrometry (LCMS) assay was firstly established and validated for confirmation analysis of the six types of drugs and/or their metabolites...
March 12, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29529176/optimization-of-physicochemical-parameters-of-tannase-post-purification-and-its-versatile-bioactivity
#8
Syed Hidayathulla, Abdelaaty A Shahat, Mansour S Alsaid, Abdullah A Al-Mishari
The present study investigates the optimization of tannase production from A. nidulans for various physicochemical parameters and harvests tannase for its chemical characterization. The maximum tannase activity was observed on the third day of incubation at 35°C and the stability was observed at pH 5.5 to 6.0 by holding its 100% activity. The tannase was partially purified from A. nidulans [FT10] by ammonium sulfate precipitation at different concentrations and it was found that at 80% of ammonium sulfate concentration, the precipitate exhibited the maximum activity for tannase of 96 U/ml...
February 26, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29527765/characterization-of-a-red-pigment-from-fusarium-chlamydosporum-exhibiting-selective-cytotoxicity-against-human-breast-cancer-mcf-7-cell-lines
#9
K Soumya, K Narasimha Murthy, G L Sreelatha, T Sharmila
AIM: This research aims to characterize the pigment produced by isolated fungi and to evaluate its anti-cancer activities. METHODS AND RESULTS: Pigment producing fungi was isolated and identified as Fusarium chlamydosporum. The pigment was extracted with chloroform, purified by PTLC and characterized by FT-IR, ESIMS, LCMS and NMR (1 HNMR,13 C NMR) spectral analysis, which revealed the pigment to be "long chain hydrocarbons with poly unsaturated groups" (m/z 702)...
March 12, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29526533/type-2-diabetes-related-proteins-derived-from-an-in-vitro-model-of-inflamed-fat-tissue
#10
Jean Paul Ten Klooster, Alexandros Sotiriou, Sjef Boeren, Stefan Vaessen, Jacques Vervoort, Raymond Pieters
Currently, there is a worldwide increase of patients with Type 2 diabetes (T2D). During the progression of healthy obese to T2D status, there is an influx of immune cells, in particular macrophages, into visceral adipose tissue, accompanied by an increase of inflammatory cytokines, such as, IL6, TNFα and Hp. To get a better insight in the underlying mechanisms, we performed a quantitative LCMS analysis on a modified in vitro assay, combining 3T3L1 adipocytes and activated RAW264.7 macrophages, thus mimicking inflamed adipose tissue...
March 8, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29524771/proposal-of-degradation-pathway-with-toxicity-prediction-for-hydrolytic-and-photolytic-degradation-products-of-timolol
#11
Prashant S Devrukhakar, Shiva Shankar M, Shankar G, Srinivas R
Timolol (TIM) is a potent β-adrenergic blocker, useful in treatment of ocular hypertension or open-angle glaucoma. Development and validation of stability indicating LCMS assay method for TIM was accomplished coherent with ICH guideline. Successful chromatographic separation of TIM with its four degradation products was attained by using gradient elution mode on reverse phase column using ammonium acetate buffer, pH 4.6 as mobile phase A and organic solvent as the mobile phase B. Chromatographic conditions were set such as 1...
February 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29505960/a-rapid-lc-ms-ms-assay-for-quantification-of-piperacillin-and-tazobactam-in-human-plasma-and-pleural-fluid-application-to-a-clinical-pharmacokinetic-study
#12
Natalia D Popowicz, Sean J O'Halloran, Deirdre Fitzgerald, Y C Gary Lee, David A Joyce
Piperacillin, in combination with tazobactam is a common first-line antibiotic used for the treatment of pleural infection, however its pleural pharmacokinetics and penetration has not previously been reported. The objective of this work was to develop and validate a rapid and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay for quantification of piperacillin (PIP) and tazobactam (TAZ). PIP and TAZ were extracted from both human plasma and pleural fluid samples by protein precipitation in methanol containing the internal standards (IS) piperacillin-d5 (PIP-d5 ) and sulbactam (SUL)...
April 1, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29490584/co-occurrence-of-mycotoxins-in-commercial-formula-milk-and-cereal-based-baby-food-in-qatar
#13
Zahoor Ul Hassan, Roda Al Thani, Fathy A Atia, Saeed Al Meer, Quirico Migheli, Samir Jaoua
The present study was conducted to explore the occurrence of mycotoxins in commercial baby foods in Doha-Qatar. LCMS/MS- and HPLC-based analysis of baby food (n=67) for 12 mycotoxins, confirmed the presence of aflatoxin M1 (AFM1, 33%), ochratoxin A (OTA, 31%), deoxynivalenol (DON, 27%), aflatoxin B1 (AFB1, 22%), fumonisin B2 (FB2, 10%), zearalenone (ZEN, 4%) and T-2 toxin (2%). Noodles exhibited the maximum contamination percentage, with 33% of the samples being contaminated above the EU maximum limits, for at least one mycotoxin...
February 28, 2018: Food Additives & Contaminants. Part B, Surveillance
https://www.readbyqxmd.com/read/29469228/proteomic-studies-on-the-swimbladder-of-the-european-eel-anguilla-anguilla
#14
Fernando J Sialana, Gabriel Schneebauer, Ana Paunkov, Bernd Pelster, Gert Lubec
The swimbladder of fish is a vital organ that with gas gland cells in the swimbladder wall enables key physiological functions including buoyancy regulation in the face of different hydrostatic pressures. Specific gas gland cells produce and secrete acidic metabolites into the blood in order to reduce the physical solubility of gases and blood gas transport capacity for regulating the volume of the swimbladder. Transcriptomic analyses have provided evidence at the RNA level but no specific studies at the protein level have been carried out so far...
February 22, 2018: Proteomics
https://www.readbyqxmd.com/read/29451338/can-dimedone-be-used-to-study-selenoproteins-an-investigation-into-the-reactivity-of-dimedone-towards-oxidized-forms-of-selenocysteine
#15
N Connor Payne, Drew R Barber, Erik L Ruggles, Robert J Hondal
Dimedone is a widely used reagent to assess the redox state of cysteine-containing proteins as it will alkylate sulfenic acid residues, but not sulfinic acid residues. While it has been reported that dimedone can label selenenic acid residues in selenoproteins, we investigated the stability, and reversibility of this label in a model peptide system. We also wondered whether dimedone could be used to detect seleninic acid residues. We used benzenesulfinic acid, benzeneseleninic acid, and model selenocysteine-containing peptides to investigate possible reactions with dimedone...
February 16, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29443058/two-photon-intravital-imaging-of-leukocytes-during-the-immune-response-in-lipopolysaccharide-treated-mouse-liver
#16
Sang A Park, Young Ho Choe, Sung Hwan Lee, Young-Min Hyun
Sepsis is a type of severe infection that can cause organ failure and tissue damage. Although the mortality and morbidity rates associated with sepsis are extremely high, no direct treatment or organ-related mechanism has been examined in detail in real time. The liver is the key organ that manages toxins and infections in the human body. Herein, we aimed to perform intravital imaging of mouse liver after induction of endotoxemia in order to track the motility of immune cells, such as neutrophils and liver capsular macrophages (LCMs)...
February 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29410121/calcium-calmodulin-dependent-protein-kinase-ii-regulation-of-iks-during-sustained-beta-adrenergic-receptor-stimulation
#17
Tyler Shugg, Derrick E Johnson, Minghai Shao, Xianyin Lai, Frank Witzmann, Theodore R Cummins, Michael Rubart-Von-der Lohe, Andy Hudmon, Brian R Overholser
BACKGROUND: Sustained β-adrenergic receptor (β-AR) stimulation causes pathophysiologic changes during heart failure (HF), including inhibition of the slow component of the delayed rectifier current, IKs. Aberrant calcium handling, including increased activation of calcium/calmodulin-dependent protein kinase II (CaMKII), contributes to arrhythmia development during HF. OBJECTIVE: To investigate CaMKII regulation of KCNQ1 (pore-forming subunit of IKs) during sustained β-AR stimulation and associated functional implications on IKs...
February 1, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/29407362/simultaneous-quantitation-of-multiple-contraceptive-hormones-in-human-serum-by-lc-ms-ms
#18
Steven W Blue, Andrea J Winchell, Amy V Kaucher, Rachel A Lieberman, Christopher T Gilles, Maria N Pyra, Renee Heffron, Xuanlin Hou, Robert W Coombs, Kavita Nanda, Nicole L Davis, Athena P Kourtis, Joshua T Herbeck, Jared M Baeten, Jairam R Lingappa, David W Erikson
OBJECTIVE: The objective was to develop a method to simultaneously quantify five commonly used hormonal contraceptives (HCs) and two endogenous sex steroids by liquid chromatography-tandem triple quadrupole mass spectrometry (LC-MS/MS) and apply this method to human serum samples. STUDY DESIGN: We developed a method to simultaneously analyze ethinyl estradiol (EE2), etonogestrel (ENG), levonorgestrel (LNG), medroxyprogesterone acetate (MPA) and norethisterone (NET), along with estradiol (E2) and progesterone (P4), in human serum for a Shimadzu Nexera-LCMS-8050 LC-MS/MS platform...
April 2018: Contraception
https://www.readbyqxmd.com/read/29402777/correlation-of-insulin-like-growth-factor-i-and-ii-concentrations-at-birth-measured-by-mass-spectrometry-and-growth-from-birth-to-two-months
#19
Colin P Hawkes, Deirdre M Murray, Louise C Kenny, Mairead Kiely, Jonathan O'B Hourihane, Alan D Irvine, Zengru Wu, Yair Argon, Richard E Reitz, Michael J McPhaul, Adda Grimberg
BACKGROUND: Immunoassays used to measure insulin-like growth factor (IGF)-I and -II concentrations are susceptible to interference from IGF-binding proteins. The aim of this study was to investigate the association of IGF-I and -II concentrations at birth with neonatal anthropometry using a liquid chromatography/mass spectrometry (LCMS) assay. METHODS: LCMS was used to measure IGF-I and -II concentrations in umbilical cord blood of term, healthy infants enrolled in the Cork BASELINE Birth Cohort Study...
January 18, 2018: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/29399292/anti-neuroinflammatory-effects-of-ethanolic-extract-of-black-chokeberry-aronia-melanocapa-l-in-lipopolysaccharide-stimulated-bv2-cells-and-icr-mice
#20
Kang Pa Lee, Nan Hee Choi, Hyun-Soo Kim, Sanghyun Ahn, In-Sik Park, Dea Won Lee
BACKGROUND/OBJECTIVES: One of the mechanisms considered to be prevalent in the development of Alzheimer's disease (AD) is hyper-stimulation of microglia. Black chokeberry (Aronia melanocapa L.) is widely used to treat diabetes and atherosclerosis, and is known to exert anti-oxidant and anti-inflammatory effects; however, its neuroprotective effects have not been elucidated thus far. MATERIALS/METHODS: We undertook to assess the anti-inflammatory effect of the ethanolic extract of black chokeberry friut (BCE) in BV2 cells, and evaluate its neuroprotective effect in the lipopolysaccharide (LPS)-induced mouse model of AD...
February 2018: Nutrition Research and Practice
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