keyword
MENU ▼
Read by QxMD icon Read
search

Lcms

keyword
https://www.readbyqxmd.com/read/29451338/can-dimedone-be-used-to-study-selenoproteins-an-investigation-into-the-reactivity-of-dimedone-towards-oxidized-forms-of-selenocysteine
#1
N Connor Payne, Drew R Barber, Erik L Ruggles, Robert J Hondal
Dimedone is a widely used reagent to assess the redox state of cysteine-containing proteins as it will alkylate sulfenic acid residues, but not sulfinic acid residues. While it has been reported that dimedone can label selenenic acid residues in selenoproteins, we investigated the stability, and reversibility of this label in a model peptide system. We also wondered whether dimedone could be used to detect seleninic acid residues. We used benzenesulfinic acid, benzeneseleninic acid, and model selenocysteine-containing peptides to investigate possible reactions with dimedone...
February 16, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29443058/two-photon-intravital-imaging-of-leukocytes-during-the-immune-response-in-lipopolysaccharide-treated-mouse-liver
#2
Sang A Park, Young Ho Choe, Sung Hwan Lee, Young-Min Hyun
Sepsis is a type of severe infection that can cause organ failure and tissue damage. Although the mortality and morbidity rates associated with sepsis are extremely high, no direct treatment or organ-related mechanism has been examined in detail in real time. The liver is the key organ that manages toxins and infections in the human body. Herein, we aimed to perform intravital imaging of mouse liver after induction of endotoxemia in order to track the motility of immune cells, such as neutrophils and liver capsular macrophages (LCMs)...
February 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29410121/calcium-calmodulin-dependent-protein-kinase-ii-regulation-of-iks-during-sustained-beta-adrenergic-receptor-stimulation
#3
Tyler Shugg, Derrick E Johnson, Minghai Shao, Xianyin Lai, Frank Witzmann, Theodore R Cummins, Michael Rubart-Von-der Lohe, Andy Hudmon, Brian R Overholser
BACKGROUND: Sustained β-adrenergic receptor (β-AR) stimulation causes pathophysiologic changes during heart failure (HF), including inhibition of the slow component of the delayed rectifier current, IKs. Aberrant calcium handling, including increased activation of calcium/calmodulin-dependent protein kinase II (CaMKII), contributes to arrhythmia development during HF. OBJECTIVE: To investigate CaMKII regulation of KCNQ1 (pore-forming subunit of IKs) during sustained β-AR stimulation and associated functional implications on IKs...
February 1, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/29407362/simultaneous-quantitation-of-multiple-contraceptive-hormones-in-human-serum-by-lc-ms-ms
#4
Steven W Blue, Andrea J Winchell, Amy V Kaucher, Rachel A Lieberman, Christopher T Gilles, Maria N Pyra, Renee Heffron, Xuanlin Hou, Robert W Coombs, Kavita Nanda, Nicole L Davis, Athena P Kourtis, Joshua T Herbeck, Jared M Baeten, Jairam R Lingappa, David W Erikson
OBJECTIVE: To develop a method to simultaneously quantify five commonly used hormonal contraceptives (HC) and 2 endogenous sex steroids by liquid chromatography-tandem triple quadrupole mass spectrometry (LC-MS/MS) and apply this method to human serum samples. STUDY DESIGN: We developed a method to simultaneously analyze ethinyl estradiol (EE2), etonogestrel (ENG), levonorgestrel (LNG), medroxyprogesterone acetate (MPA), and norethisterone (NET), along with estradiol (E2) and progesterone (P4) in human serum for a Shimadzu Nexera-LCMS-8050 LC-MS/MS platform...
January 30, 2018: Contraception
https://www.readbyqxmd.com/read/29402777/correlation-of-insulin-like-growth-factor-i-and-ii-concentrations-at-birth-measured-by-mass-spectrometry-and-growth-from-birth-to-two-months
#5
Colin P Hawkes, Deirdre M Murray, Louise C Kenny, Mairead Kiely, Jonathan O'B Hourihane, Alan D Irvine, Zengru Wu, Yair Argon, Richard E Reitz, Michael J McPhaul, Adda Grimberg
BACKGROUND: Immunoassays used to measure insulin-like growth factor (IGF)-I and -II concentrations are susceptible to interference from IGF-binding proteins. The aim of this study was to investigate the association of IGF-I and -II concentrations at birth with neonatal anthropometry using a liquid chromatography/mass spectrometry (LCMS) assay. METHODS: LCMS was used to measure IGF-I and -II concentrations in umbilical cord blood of term, healthy infants enrolled in the Cork BASELINE Birth Cohort Study...
January 18, 2018: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/29399292/anti-neuroinflammatory-effects-of-ethanolic-extract-of-black-chokeberry-aronia-melanocapa-l-in-lipopolysaccharide-stimulated-bv2-cells-and-icr-mice
#6
Kang Pa Lee, Nan Hee Choi, Hyun-Soo Kim, Sanghyun Ahn, In-Sik Park, Dea Won Lee
BACKGROUND/OBJECTIVES: One of the mechanisms considered to be prevalent in the development of Alzheimer's disease (AD) is hyper-stimulation of microglia. Black chokeberry (Aronia melanocapa L.) is widely used to treat diabetes and atherosclerosis, and is known to exert anti-oxidant and anti-inflammatory effects; however, its neuroprotective effects have not been elucidated thus far. MATERIALS/METHODS: We undertook to assess the anti-inflammatory effect of the ethanolic extract of black chokeberry friut (BCE) in BV2 cells, and evaluate its neuroprotective effect in the lipopolysaccharide (LPS)-induced mouse model of AD...
February 2018: Nutrition Research and Practice
https://www.readbyqxmd.com/read/29372474/optimal-conditions-and-the-advantages-of-using-laser-microdissection-and-liquid-chromatography-tandem-mass-spectrometry-for-diagnosing-renal-amyloidosis
#7
Michiko Aoki, Dedong Kang, Akira Katayama, Naomi Kuwahara, Shinya Nagasaka, Yoko Endo, Mika Terasaki, Shinobu Kunugi, Yasuhiro Terasaki, Akira Shimizu
BACKGROUND: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has recently been utilized to accurately detect the amyloid proteins of renal amyloidosis. The present study investigated the optimal procedures for analyzing samples by LCMS/MS, and the advantage of using this technique to diagnosis renal amyloidosis. METHODS: To detect amyloid proteins, laser microdissected glomeruli from AL (n = 13) or AA (n = 10) renal amyloidosis patients were digested and analyzed by LCMS/MS...
January 25, 2018: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/29329238/long-chain-metabolites-of-vitamin-e-metabolic-activation-as-a-general-concept-for-lipid-soluble-vitamins
#8
REVIEW
Martin Schubert, Stefan Kluge, Lisa Schmölz, Maria Wallert, Francesco Galli, Marc Birringer, Stefan Lorkowski
Vitamins E, A, D and K comprise the class of lipid-soluble vitamins. For vitamins A and D, a metabolic conversion of precursors to active metabolites has already been described. During the metabolism of vitamin E, the long-chain metabolites (LCMs) 13'-hydroxychromanol (13'-OH) and 13'-carboxychromanol (13'-COOH) are formed by oxidative modification of the side-chain. The occurrence of these metabolites in human serum indicates a physiological relevance. Indeed, effects of the LCMs on lipid metabolism, apoptosis, proliferation and inflammatory actions as well as tocopherol and xenobiotic metabolism have been shown...
January 12, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29325818/simultaneous-determination-of-voriconazole-posaconazole-itraconazole-and-hydroxy-itraconazole-in-human-plasma-using-lcms-ms
#9
Nicole Jenkins, Marion Black, Hans G Schneider
INTRODUCTION: Invasive fungal infections are an increasing cause of mortality and morbidity in high risk patient populations such as those on immunosuppressive therapy. Triazole antifungals are recommended for the prevention and treatment of such infections. The aim of this study was to develop and validate a simple, sensitive and robust LCMS/MS method for the simultaneous analysis in human plasma of three frequently used antifungal drugs: voriconazole, posaconazole, and itraconazole...
January 9, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29324787/association-of-sex-hormones-with-physical-laboratory-and-imaging-markers-of-anthropometry-in-men-and-women-from-the-general-population
#10
Tom Seyfart, Nele Friedrich, Hanna Kische, Robin Bülow, Henri Wallaschofski, Henry Völzke, Matthias Nauck, Brian G Keevil, Robin Haring
OBJECTIVES: The aim of this study was to evaluate the association of sex hormones with anthropometry in a large population-based cohort, with liquid chromatography-mass spectrometry (LCMS)-based sex hormone measurements and imaging markers. STUDY DESIGN/MAIN OUTCOME MEASURES: Cross-sectional data from 957 men and women from the population-based Study of Health in Pomerania (SHIP) were used. Associations of a comprehensive panel of LCMS-measured sex hormones with anthropometric parameters, laboratory, and imaging markers were analyzed in multivariable regression models for the full sample and stratified by sex...
2018: PloS One
https://www.readbyqxmd.com/read/29311077/posaconazole-tablets-in-real-life-lung-transplantation-impact-on-exposure-drug-drug-interactions-and-drug-management-in-lung-transplanted-patients-including-cystic-fibrosis
#11
Manon Launay, Antoine Roux, Laurence Beaumont, Benoit Douvry, Lucien Lecuyer, Emmanuel Douez, Clément Picard, Dominique Grenet, Vincent Jullien, Véronique Boussaud, Romain Guillemain, Eliane M Billaud
Appropriate exposure to Posaconazole (PSZ) was limited since the recent approval of the delayed release oral tablet formulation. Our goal was to determine the exposure obtained by using the standard dose of 300mgx1 in lung transplant patients (LT) including cystic fibrosis (CF) background.PSZ trough concentrations (C0) were determined using LCMS assay. Indicative thresholds of interest were <0.7mg/L (prophylaxis) and 1-3mg/L (curative). Tacrolimus (TRL) and everolimus (ERL) C0 measured during PSZ exposure were collected...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29306631/peptide-retention-time-prediction-in-hydrophilic-interaction-liquid-chromatography-comparison-of-separation-selectivity-between-bare-silica-and-bonded-stationary-phases
#12
Victor Spicer, Oleg V Krokhin
Peptide separation selectivity of four different HILIC sorbents was compared through the development of sequence-specific retention prediction algorithms for the datasets of 36-40 thousands peptides each. Hydrophilicity of these sorbents at pH 4.5 (peptide retention under acetonitrile:water gradients) increases in the following order: Luna HILIC < XBridge Amide < Atlantis Silica ∼ Luna HILIC Silica. Bare silica phases are characterized by higher retention coefficients for basic residues (Arg, Lys, His), while interactions with neutral HILIC phases is driven by interaction with the charged residues (Asp, Glu, Arg, Lys, His)...
December 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29303546/establishment-of-lcms-based-platform-for-discovery-of-quorum-sensing-inhibitors-signal-detection-in-pseudomonas-aeruginosa-pao1
#13
Manoj Kushwaha, Shreyans K Jain, Nisha Sharma, Vidushi Abrol, Sundeep Jaglan, Ram A Vishwakarma
Targeting the main three networking systems viz. Las, RhI and PQS via natural quenchers is a new ray of hope for combating the persistent behavior of Pseudomonas aeruginosa. In the bacterial chemical vocabulary pyocyanin, N-AHLs and rhamnolipids are the main keywords, which are responsible for the social and nomadic behavior of P. aeruginosa. In the present work LCMS-based real-time qualitative and quantitative analysis of pyocyanin, green phenazine, N-AHLs, and rhamnolipids were performed. The quantitative analysis indicates that the production of pyocyanin and NHSLs increases with time while the production of rhamnolipids discontinued after 16 Hrs...
January 5, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29248726/integrative-analysis-of-rewired-central-metabolism-in-temozolomide-resistant-cells
#14
Selva Rupa C Immanuel, Avinash D Ghanate, Dharmeshkumar S Parmar, Fiona Marriage, Venkateshwarlu Panchagnula, Philip J Day, Anu Raghunathan
An authenticated U87MG clonal glioblastoma cell line was investigated to identify a sub-population of neurospheroidal (NSP) cells within the main epithelial population (U87MG). The NSP cells sorted using Fluorescence Assisted Cell Sorting (FACS) showed varied morphology, 30% lower growth rates, 40% higher IC50 values for temozolomide drug and could differentiate into the glial cell type (NDx). Metabolite profiling using HR-LCMS identified glucose, glutamine and serine in both populations and tryptophan only in U87MG as growth limiting substrates...
December 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29247738/fatty-acid-binding-protein-5-mediates-the-uptake-of-fatty-acids-but-not-drugs-into-human-brain-endothelial-cells
#15
Gordon S Lee, Yijun Pan, Martin J Scanlon, Christopher J H Porter, Joseph A Nicolazzo
The purpose of this study was to examine the involvement of fatty acid binding protein 5 (FABP5), a lipid binding protein expressed at the blood-brain barrier (BBB), in fatty acid and drug uptake into human brain endothelial cells. Following transfection with siRNA against hFABP5, human brain endothelial cell (hCMEC/D3) uptake of lipophilic ligands with varying affinity to FABP5 was assessed with intracellular concentrations quantified by liquid scintillation counting, HPLC or LCMS/MS. The in situ BBB transport of [3H]-diazepam was also assessed in wild type (WT) and FABP5-deficient mice...
December 13, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29238962/population-pharmacokinetics-of-polymyxin-b
#16
Pooja Manchandani, Visanu Thamlikitkul, Yanina Dubrovskaya, Jessica T Babic, David C Lye, Lawrence S Lee, Vincent H Tam
Polymyxin B is used as a last treatment resort for multidrug-resistant Gram-negative bacterial infections. The objectives of this study were to examine the population pharmacokinetics of polymyxin B and investigate factor(s) influencing pharmacokinetic variability. Four serial blood samples each were collected from 35 adult patients at steady state. The concentrations of individual polymyxin B components were analyzed using a validated liquid chromatography / tandem mass spectrometry assay and combined to derive total concentrations...
December 14, 2017: Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29217478/identification-of-a-gene-encoding-a-flavoprotein-involved-in-bile-acid-metabolism-by-the-human-gut-bacterium-clostridium-scindens-atcc-35704
#17
Spencer C Harris, Saravanan Devendran, João M P Alves, Sean M Mythen, Phillip B Hylemon, Jason M Ridlon
BACKGROUND: The multi-step bile acid 7α-dehydroxylating pathway by which a few species of Clostridium convert host primary bile acids to toxic secondary bile acids is of great importance to gut microbiome structure and host physiology and disease. While genes in the oxidative arm of the 7α-dehydroxylating pathway have been identified, genes in the reductive arm of the pathway are still obscure. METHODS: We identified a candidate flavoprotein-encoding gene predicted to metabolize steroids...
December 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29205053/2017-white-paper-rise-of-hybrid-lba-lcms-immunogenicity-assays-part-2-hybrid-lba-lcms-biotherapeutics-biomarkers-immunogenicity-assays-and-regulatory-agencies-inputs
#18
Hendrik Neubert, An Song, Anita Lee, Cong Wei, Jeff Duggan, Keyang Xu, Eric Woolf, Chris Evans, Joe Palandra, Omar Laterza, Shashi Amur, Isabella Berger, Mark Bustard, Mark Cancilla, Shang-Chiung Chen, Seongeun Julia Cho, Eugene Ciccimaro, Isabelle Cludts, Laurent Cocea, Celia D'Arienzo, Lieza Danan-Leon, Lorella Di Donato, Fabio Garofolo, Sam Haidar, Akiko Ishii-Watabe, Hao Jiang, John Kadavil, Sean Kassim, Pekka Kurki, Olivier Le Blaye, Kai Liu, Rod Mathews, Gustavo Mendes Lima Santos, Makoto Niwa, João Pedras-Vasconcelos, Mark Qian, Brian Rago, Ola Saad, Yoshiro Saito, Natasha Savoie, Dian Su, Matthew Szapacs, Nilufer Tampal, Stephen Vinter, Jian Wang, Jan Welink, Emma Whale, Amanda Wilson, Y-J Xue
The 2017 11th Workshop on Recent Issues in Bioanalysis (11th WRIB) took place in Los Angeles/Universal City, California on 3-7 April 2017 with participation of close to 750 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event - a full immersion week of bioanalysis, biomarkers and immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecule analysis involving LCMS, hybrid ligand binding assay (LBA)/LCMS and LBA approaches...
December 2017: Bioanalysis
https://www.readbyqxmd.com/read/29190577/ctab-and-sds-assisted-facile-fabrication-of-sno2-nanoparticles-for-effective-degradation-of-carbamazepine-from-aqueous-phase-a-systematic-and-comparative-study-of-their-degradation-performance
#19
Shamima Begum, M Ahmaruzzaman
In the present study, SnO2 nanoparticles were successfully synthesized by chemical precipitation method using anhydrous aspartic acid and surfactant at two annealing temperatures, 300 °C and 600 °C. The effect of surfactants cationic CTAB and anionic SDS on the synthesized SnO2 nanoparticles (NPs) were studied elaborately. In this article, for the first time, SnO2 NPs were employed as an excellent photocatalyst in the degradation of carbamazepine (CBZ), a popular antiepileptic drug which is most commonly detected pharmaceutically active compounds (PhACs) in municipal wastewater under UV-C light irradiation...
November 15, 2017: Water Research
https://www.readbyqxmd.com/read/29175554/determination-of-zstk474-a-novel-pan-pi3k-inhibitor-in-mouse-plasma-by-lc-ms-ms-and-its-application-to-pharmacokinetics
#20
Amarinder Singh, Thanusha Thatikonda, Amit Kumar, Priya Wazir, Vijayabhaskar V, Utpal Nandi, Parvinder Pal Singh, Surjeet Singh, Ajay Prakash Gupta, Manoj K Tikoo, Gurdarshan Singh, Ram Vishwakarma
ZSTK474, a promising novel anticancer molecule derived from s-triazine, found to have antitumor activities against different cancer cell lines. However, neither LCMS method nor pharmacokinetics of ZSTK474 has been reported till now. A sensitive, simple, short and specific liquid chromatography tandem mass spectrometry (LCMS/MS) method was developed for the quantification of ZSTK474 in mouse plasma accordance with the US Food and Drug Administration guidelines. Extraction of drug molecule was carried out using protein precipitation...
November 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
keyword
keyword
66679
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"