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https://www.readbyqxmd.com/read/28076774/chromatographic-efficiency-and-selectivity-in-top-down-proteomics-of-histones
#1
Yiyang Zhou, Ximo Zhang, Luca Fornelli, Philip D Compton, Neil Kelleher, Mary J Wirth
Histones are involved in epigenetic control of a wide variety of cellular processes through their multiple post-translational modifications. Their strongly cationic nature makes them challenging to separate with reversed-phase liquid chromatography coupled to mass spectrometry (RPLC-MS), where trifluoroacetic acid is avoided due to adduct formation. Columns with higher resolution are needed. In this work, RPLC-MS is performed on a histone sample using difluoroacetic acid and a 20-min gradient. Columns with C18 surfaces are compared for two different types of particle morphologies: 1) fully porous particles of 5μm in diameter, 2) superficially porous particles of 3μm in diameter with a shell of 0...
December 23, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28067053/global-profiling-of-metabolite-and-lipid-soluble-microbial-products-in-anaerobic-wastewater-reactor-supernatant-using-uplc-mse
#2
Phornpimon Tipthara, Chinagarn Kunacheva, Yan Ni Annie Soh, Stephen C C Wong, Ng Sean Pin, David C Stuckey, Bernhard O Boehm
Identification of soluble microbial products (SMPs) released during bacterial metabolism in mixed cultures in bioreactors is essential to understanding fundamental mechanisms of their biological production. SMPs constitute one of the main foulants (together with colloids and bacterial flocs) in membrane bioreactors widely used to treat, and ultimately recycle wastewater. More importantly, the composition and origin of potentially toxic, carcinogenic, or mutagenic SMPs in renewable/reused water supplies must be determined and controlled...
January 9, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28063411/the-properties-of-red-seaweed-kappaphycus-alvarezii-and-its-effect-on-mammary-carcinogenesis
#3
Vi-Sion Chang, Patrick N Okechukwu, Swee-Sen Teo
The edible red seaweed (Kappaphycus alvarezii) is one of the algae species which was found to be rich in nutrients and nutraceutical. Hence, K. alvarezii may have the ability to suppress cancer through its antiproliferative properties. The aim of this study was to investigate the potential compounds of K. alvarezii, cytotoxicity properties of K. alvarezii extract on breast cancer cell line (MCF-7), investigated toxicity effect of high dosage K. alvarezii extract in rats and determined the effect of K. alvarezii on 7, 12-dimethylbenz[a]anthracene (DMBA) mammary carcinogenesis in rats...
January 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28061865/proteomic-analysis-of-enterotoxigenic-escherichia-coli-etec-in-neutral-and-alkaline-conditions
#4
Lucia Gonzales-Siles, Roger Karlsson, Diarmuid Kenny, Anders Karlsson, Åsa Sjöling
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and travelers to endemic areas. Secretion of the heat labile AB5 toxin (LT) is induced by alkaline conditions. In this study, we determined the surface proteome of ETEC exposed to alkaline conditions (pH 9) as compared to neutral conditions (pH 7) using a LPI Hexalane FlowCell combined with quantitative proteomics. Relative quantitation with isobaric labeling (TMT) was used to compare peptide abundance and their corresponding proteins in multiple samples at MS/MS level...
January 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28061410/photocatalytic-oxidation-of-trace-carbamazepine-in-aqueous-solution-by-visible-light-driven-znln2s4-performance-and-mechanism
#5
Longli Bo, Kebo He, Na Tan, Bo Gao, Qiqi Feng, Jiadong Liu, Lei Wang
In this work, visible-light-driven Znln2S4 photocatalyst (band gap 1.98 eV, absorption wavelength 300-600 nm) was synthesized by a facile hydrothermal method for photocatalytic degradation of trace pharmaceutical carbamazepine (CBZ) in aqueous solution, and characterized by UV-Vis diffusive reflectance spectroscopy, BET, SEM and XRD, respectively. It demonstrated that a complete degradation of CBZ was achieved with an optimal Znln2S4 dosage of 30 mg/L under a 100 W iodine-gallium lamp irradiation of 20 min, which afforded the reaction rate constant and half-life being respectively 10...
January 3, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28052759/exploring-the-use-of-routinely-available-retrospective-data-to-study-the-association-between-malaria-control-scale-up-and-micro-economic-outcomes-in-zambia
#6
Alison Comfort, Anthony Leegwater, Sharon Nakhimovsky, Henry Kansembe, Busiku Hamainza, Benson Bwalya, Martin Alilio, Ben Johns, Lauren Olsho
BACKGROUND: Country-level evidence on the impact of malaria control on micro-economic outcomes is vital for mobilizing domestic and donor resources for malaria control. Using routinely available survey data could facilitate this investigation in a cost-efficient way. METHODS: The authors used Malaria Indicator Surveys (MIS) and Living Conditions Monitoring Survey (LCMS) data from 2006 to 2010 for all 72 districts in Zambia to relate malaria control scale-up with household food spending (proxy for household well-being), educational attainment and agricultural production...
January 4, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28035802/rapid-distinction-of-leucine-and-isoleucine-in-monoclonal-antibodies-using-nanoflow-lcms-n
#7
Dhanashri Bagal, Eddie Kast, Ping Cao
Monoclonal antibodies (mAbs) are large heterogeneous molecules that represent a growing class of therapeutics. De novo sequencing of mAbs becomes necessary when the original cell line or the cDNA is unavailable. An important feature in sequencing of mAbs is the discrimination of isobaric residues (Xle): leucine (Leu) and isoleucine (Ile). An incorrect identification of the Xle site, especially in the complementarity determining regions (CDRs), can result in the production of an antibody with severely compromised efficacy...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28029204/translating-thermal-response-of-triblock-copolymer-assemblies-in-dilute-solution-to-macroscopic-gelation-and-phase-separation
#8
Zhe Sun, Ye Tian, Wendy L Hom, Oleg Gang, Surita R Bhatia, Robert B Grubbs
The thermal response of semi-dilute solutions (5 w/w%) of two amphiphilic thermoresponsive poly(ethylene oxide)-b-poly(N,N-diethylacrylamide)-b-poly(N,N-dibutylacrylamide) (PEO45 -PDEAmx -PDBAm12 ) triblock copolymers, which differ only in the size of the central responsive block, in water was examined. Aqueous PEO45 -PDEAm41 -PDBAm12 solutions, which undergo a thermally induced sphere-to-worm transition in dilute solution, were found to reversibly form soft (G'≈10 Pa) free-standing physical gels after 10 min at 55 °C...
December 28, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/28013465/decomposition-of-3-5-dinitrobenzamide-in-aqueous-solution-during-uv-h2o2-and-uv-tio2-oxidation-processes
#9
Yingjie Yan, Qi-Nan Liao, Feng Ji, Wei Wang, Shoujun Yuan, Zhen-Hu Hu
3,5-Dinitrobenzamide has been widely used as a feed additive to control coccidiosis in poultry, and part of the added 3,5-dinitrobenzamide is excreted into wastewater and surface water. The removal of 3,5-dinitrobenzamide from wastewater and surface water has not been reported in previous studies. Highly reactive hydroxyl radicals from UV/hydrogen peroxide (H2O2) and UV/titanium dioxide (TiO2) advanced oxidation processes (AOPs) can decompose organic contaminants efficiently. In this study, the decomposition of 3,5-dinitrobenzamide in aqueous solution during UV/H2O2 and UV/TiO2 oxidation processes was investigated...
December 24, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28013407/proteomic-based-biomarker-discovery-for-development-of-next-generation-diagnostics
#10
REVIEW
Akbar Khalilpour, Tugba Kilic, Saba Khalilpour, Mario Moisés Álvarez, Iman K Yazdi
In the post-genome age, proteomics is receiving significant attention because they provide an invaluable source of biological structures and functions at the protein level. The search for disease-specific biomarkers for diagnostic and/or therapeutic applications is one of the areas that proteomics is having a significant impact. Thus, the identification of a "good" biomarker enables a more accurate early diagnosis and prognosis of disease. Rapid advancements in mass spectrometry (MS) instrumentation, liquid chromatography MS (LCMS), protein microarray technology, and other protein profiling methodologies have a substantial expansion of our toolbox to identify disease-specific protein and peptide biomarkers...
January 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27984696/trapping-reactions-of-the-sulfenyl-and-sulfinyl-tautomers-of-sulfenic-acids
#11
Murugaeson R Kumar, Patrick J Farmer
Sulfenic acids react as both nucleophiles and electrophiles, which may be attributable to interconversion between sulfenyl and sulfinyl tautomers. We demonstrate one-pot trapping of both tautomeric forms of glutathione sulfenic acid by LCMS. The sulfinyl tautomers are characterized by reaction with nucleophilic reagents such as dimedone and cyanide, giving unique products that are analogous to corresponding adducts of aldehydes. Likewise, we show that aldehyde reactive reagents such as silyl enol ethers also react with glutathione sulfenic acid to give products characteristic of both sulfenyl and sulfinyl tautomers...
December 28, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27936436/spectroscopic-single-crystal-x-ray-hirshfeld-in-vitro-and-in-silico-biological-evaluation-of-a-new-series-of-potent-thiazole-nucleus-integrated-with-pyrazoline-scaffolds
#12
Vinutha V Salian, Badiadka Narayana, Balladka K Sarojini, Madan S Kumar, Govinahalli S Nagananda, Kullaiah Byrappa, Avinash K Kudva
In the present study, the spectroscopic characterization of a new series of substituted thiazole linked pyrazoline scaffolds 4a-l was performed. The formation of 4a-l from the intermediate 3-(4-chlorophenyl)-5-[4-(propan-2-yl)phenyl]-4,5-dihydro-1H-pyrazole-1-carbothioamide 2 and substituted 2-bromo-1-phenylethanone 3a-l was evidenced through the changes in FTIR, (1)H NMR, (13)C NMR, LCMS data. The X-ray diffraction studies revealed that compound 2 and 4g crystallized in monoclinic crystal system with P21/n space group...
November 28, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27925239/physiologically-based-pharmacokinetic-modeling-revealed-minimal-codeine-intestinal-metabolism-in-first-pass-removal-in-rats
#13
Keumhan Noh, Shu Chen, Qi J Yang, K Sandy Pang
The physiologically-based model with segregated flow to the intestine (SFM-PBPK; partial, lower flow to enterocyte region vs. greater flow to serosal region) was found to describe first-pass glucuronidation of morphine (M) to morphine-3β-glucuronide (MG) in rats after intraduodenal (ID) and intravenous (IV) administration better than the traditional model (TM), for which a single intestinal flow perfused the whole of the intestinal tissue. The SFM described a greater extent of intestinal M glucuronidation for ID vs...
December 7, 2016: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/27889674/design-synthesis-characterization-and-cation-sensing-behavior-of-amino-naphthoquinone-receptor-selective-colorimetric-sensing-of-cu-ii-ion-in-nearly-aqueous-solution-with-mimicking-logic-gate-operation
#14
C Parthiban, Kuppanagounder P Elango
An amino-naphthoquione receptor (R1) has been rationally designed, synthesized and characterized using (1)H and (13)C NMR, LCMS and single crystal X-ray diffraction studies. The receptor exhibits an instantaneous colour change from yellow to blue selectively with Cu(II) ions in water-DMF (98:2% v/v) medium. The results of UV-Vis and fluorescence spectral studies indicates that the mechanism of sensing involves formation of a 1:1 complex between R1 and Cu(II) ion. The proposed mechanism has been confirmed through product analysis using FT-IR, UV-Vis, EPR and HRMS studies in addition to magnetic moment and elemental analysis measurements...
November 18, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27867266/a-new-antifungal-isocoumarin-from-the-endophytic-fungus-trichoderma-sp-09-of-myoporum-bontioides-a-gray
#15
Wensheng Li, Jiaxin Xu, Fenqi Li, Li Xu, Chunyuan Li
BACKGROUND: Myoporum bontioides A. Gray is a commonly used medicinal plant in China. Recently, the chemical and bioactive investigations to the endophytic fungi of this plant have led to several new compounds with antimicrobial and cytotoxic activities. To find out more active molecules, the metabolites of an endophytic fungus, Trichoderma sp. 09 from the root of Myoporum bontioides were investigated. MATERIALS AND METHODS: The metabolites were isolated by column chromatography on silica gel, and their structures were elucidated on the basis of spectroscopic analysis[one-dimensional (1D), two-dimensional (2D)-nuclear magnetic resonance (NMR), Mass spectrometry (MS)], and by comparison with the published data...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27862978/abcc3-polymorphisms-and-mrna-expression-influence-the-concentration-of-a-carboxylic-acid-metabolite-in-patients-on-clopidogrel-and-aspirin-therapy
#16
André Ducati Luchessi, Marta Concheiro, Juliana de Freitas Germano, Vivian Nogueira Silbiger, Raul Hernandes Bortolin, Angelines Cruz, Oscar Quintela, Maria Brion, Angel Carracedo, Andres Iñiguez, Marisol Bravo, Manuel López-Rivadulla, Rosario Dominguez Crespo Hirata, Amanda Guerra Moraes Rego Sousa, Mario Hiroyuki Hirata
Acetylsalicylic acid and clopidogrel combined therapy has been reported to be beneficial in patients with acute coronary syndrome (ACS). Antiplatelet drug resistance, especially to clopidogrel, is a multifactorial phenomenon that affects a large number of ACS patients. The genetic contribution to this drug response is not fully elucidated. We investigated the relationship of ABC-type efflux subfamily C member 3 (ABCC3) polymorphisms and mRNA expression with plasma concentrations of clopidogrel, salicylic acid (SA), and a carboxylic acid metabolite (CAM)...
November 12, 2016: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27855512/2016-white-paper-on-recent-issues-in-bioanalysis-focus-on-biomarker-assay-validation-bav-part-3-lba-biomarkers-and-immunogenicity
#17
Susan Richards, Lakshmi Amaravadi, Renuka Pillutla, Herbert Birnboeck, Albert Torri, Kyra J Cowan, Apollon Papadimitriou, Fabio Garofolo, Christina Satterwhite, Steven Piccoli, Bonnie Wu, Corinna Krinos-Fiorotti, John Allinson, Flora Berisha, Laurent Cocea, Stephanie Croft, Stephanie Fraser, Fabrizio Galliccia, Boris Gorovits, Swati Gupta, Vinita Gupta, Sam Haidar, Charles Hottenstein, Akiko Ishii-Watabe, Darshana Jani, John Kadavil, John Kamerud, Daniel Kramer, Virginia Litwin, Gustavo Mendes Lima Santos, Robert Nelson, Yan Ni, João Pedras-Vasconcelos, Yongchang Qiu, Paul Rhyne, Afshin Safavi, Yoshiro Saito, Natasha Savoie, Kara Scheibner, Eginhard Schick, Patricia Y Siguenza, John Smeraglia, Roland F Staack, Meena Subramanyam, Giane Sumner, Theingi Thway, David Uhlinger, Martin Ullmann, Alessandra Vitaliti, Jan Welink, Chan C Whiting, Li Xue, Rong Zeng
The 2016 10th Workshop on Recent Issues in Bioanalysis (10th WRIB) took place in Orlando, Florida with participation of close to 700 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations, and regulatory agencies worldwide. WRIB was once again a weeklong event - A Full Immersion Week of Bioanalysis for PK, Biomarkers and Immunogenicity. As usual, it is specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecules involving LCMS, hybrid LBA/LCMS, and LBA approaches, with the focus on PK, biomarkers and immunogenicity...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27855509/2016-white-paper-on-recent-issues-in-bioanalysis-focus-on-biomarker-assay-validation-bav-part-2-hybrid-lba-lcms-and-input-from-regulatory-agencies
#18
An Song, Anita Lee, Fabio Garofolo, Surinder Kaur, Jeff Duggan, Christopher Evans, Joe Palandra, Lorella Di Donato, Keyang Xu, Ronald Bauer, Mark Bustard, Linzhi Chen, Laurent Cocea, Stephanie Croft, Fabrizio Galliccia, Sam Haidar, Nicola Hughes, Akiko Ishii-Watabe, Rafiqul Islam, Barry Jones, John Kadavil, Carsten Krantz, Gustavo Mendes Lima Santos, Timothy Olah, João Pedras-Vasconcelos, Ludovicus Staelens, Yoshiro Saito, Natasha Savoie, Kara Scheibner, Susan Spitz, Nilufer Tampal, Eric Thomas, Stephen Vinter, Jason Wakelin-Smith, Jan Welink, Jianing Zeng, Shaolian Zhou
The 2016 10th Workshop on Recent Issues in Bioanalysis (10(th) WRIB) took place in Orlando, Florida with participation of close to 700 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations, and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event - A Full Immersion Week of Bioanalysis including Biomarkers and Immunogenicity. As usual, it is specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecules involving LCMS, hybrid LBA/LCMS, and LBA approaches, with the focus on biomarkers and immunogenicity...
December 2016: Bioanalysis
https://www.readbyqxmd.com/read/27832539/profiling-changes-in-histone-post-translational-modifications-by-top-down-mass-spectrometry
#19
Mowei Zhou, Si Wu, David L Stenoien, Zhaorui Zhang, Lanelle Connolly, Michael Freitag, Ljiljana Paša-Tolić
Top-down mass spectrometry is a valuable tool for understanding gene expression through characterization of combinatorial histone post-translational modifications (i.e., histone code). In this protocol, we describe a top-down workflow that employs liquid chromatography (LC) coupled to mass spectrometry (MS), for fast global profiling of changes in histone proteoforms, and apply LCMS top-down approach for comparative analysis of a wild-type and a mutant fungal species. The proteoforms exhibiting differential abundances can be subjected to further targeted studies by other MS or orthogonal (e...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27826811/inducible-de-novo-biosynthesis-of-isoflavonoids-in-soybean-leaves-by-spodoptera-litura-derived-elicitors-tracer-techniques-aided-by-high-resolution-lcms
#20
Ryu Nakata, Yuki Kimura, Kenta Aoki, Naoko Yoshinaga, Masayoshi Teraishi, Yutaka Okumoto, Alisa Huffaker, Eric A Schmelz, Naoki Mori
Isoflavonoids are a characteristic family of natural products in legumes known to mediate a range of plant-biotic interactions. For example, in soybean (Glycine max: Fabaceae) multiple isoflavones are induced and accumulate in leaves following attack by Spodoptera litura (Lepidoptera: Noctuidae) larvae. To quantitatively examine patterns of activated de novo biosynthesis, soybean (Var. Enrei) leaves were treated with a combination of plant defense elicitors present in S. litura gut content extracts and L-α-[(13)C9, (15)N]phenylalanine as a traceable isoflavonoid precursor...
December 2016: Journal of Chemical Ecology
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