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https://www.readbyqxmd.com/read/28192125/clinical-implications-for-biochemical-diagnostic-thresholds-of-adrenal-sufficiency-using-a-highly-specific-cortisol-immunoassay
#1
G A Kline, J Buse, R D Krause
OBJECTIVES: Recent guidelines recommend a diagnosis of adrenal insufficiency when a stimulated peak cortisol level falls below 500nmol/L. This may not be valid using a highly specific cortisol immunoassay or liquid chromatography-mass spectroscopy (LCMS/MS). We sought to determine the diagnostic threshold for adrenal insufficiency using a new and widely available, highly specific cortisol immunoassay. DESIGN: All patients having a dynamic test of adrenal reserve had results measured using the historical cortisol assay (Roche Cortisol) and the newer assay (Roche Cortisol II)...
February 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28178633/a-novel-lcmsms-method-for-quantitative-measurement-of-short-chain-fatty-acids-in-human-stool-derivatized-with-12-c-and-13-c-labelled-aniline
#2
James Chun Yip Chan, Dorinda Yan Qin Kioh, Gaik Chin Yap, Bee Wah Lee, Eric Chun Yong Chan
A novel liquid chromatography tandem mass spectrometry (LCMSMS) method for the quantitative measurement of gut microbial-derived short-chain fatty acids (SCFAs) in human infant stool has been developed and validated. Baseline chromatographic resolution was achieved for 12 SCFAs (acetic, butyric, caproic, 2,2-dimethylbutyric, 2-ethylbutyric, isobutyric, isovaleric, 2-methylbutyric, 4-methylvaleric, propionic, pivalic and valeric acids) within an analysis time of 15min. A novel sequential derivatization of endogenous and spiked SCFAs in stool via (12)C- and (13)C-aniline respectively, facilitated the accurate quantitation of (12)C-aniline derivatized endogenous SCFAs based on calibration of exogenously (13)C-derivatized SCFAs...
January 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28170007/lactucaxanthin-a-potential-anti-diabetic-carotenoid-from-lettuce-lactuca-sativa-inhibits-%C3%AE-amylase-and-%C3%AE-glucosidase-activity-in-vitro-and-in-diabetic-rats
#3
Sowmya Shree Gopal, Magisetty Jhansi Lakshmi, Gurunathan Sharavana, Gunaseelan Sathaiah, Yadahally N Sreerama, Vallikannan Baskaran
Intestinal and pancreatic α-amylase and α-glucosidase inhibitors offer an approach to lower the levels of post-prandial hyperglycemia through the control of dietary starch breakdown in digestion. This study hypothesized that lactucaxanthin (Lxn) in lettuce (Lactuca sativa) inhibits the activity of α-amylase and α-glucosidase. In this study, the interaction of Lxn with α-amylase and α-glucosidase in silico and its inhibitory effect on these enzymes were studied using in vitro and STZ-induced diabetic rat models...
February 7, 2017: Food & Function
https://www.readbyqxmd.com/read/28166749/antidiabetic-effects-of-brucea-javanica-seeds-in-type-2-diabetic-rats
#4
Abdulwali Ablat, Mohammed Farouq Halabi, Jamaludin Mohamad, Muhammad Hafiz Husna Hasnan, Hazrina Hazni, Ser-Huy Teh, Jamil A Shilpi, Zulqarnain Mohamed, Khalijah Awang
BACKGROUND: Brucea javanica (B. javanica) seeds, also known as "Melada pahit" in Indo-Malay region are traditionally used to treat diabetes. The objective of this study was to determine antidiabetic, antioxidant and anti-inflammatory effects of B. javanica seeds on nicotinamide (NA)-streptozotocin (STZ) induced type 2 diabetic (T2D) rats and to analyze its chemical composition that correlate with their pharmacological activities. METHODS: A hydroethanolic extract of B...
February 6, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28159706/venomics-of-naja-sputatrix-the-javan-spitting-cobra-a-short-neurotoxin-driven-venom-needing-improved-antivenom-neutralization
#5
Nget Hong Tan, Kin Ying Wong, Choo Hock Tan
: The venom proteome of Naja sputatrix (Javan spitting cobra) was elucidated through reverse-phase HPLC, nano-ESI-LCMS/MS and data mining. A total of 97 distinct protein forms belonging to 14 families were identified. The most abundant proteins are the three-finger toxins (3FTXs, 64.22%) and phospholipase A2 (PLA2, 31.24%), followed by nerve growth factors (1.82%), snake venom metalloproteinase (1.33%) and several proteins of lower abundance (<1%) including a variety of venom enzymes...
January 31, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28124252/identification-of-brassinosteroid-signaling-complexes-by-coimmunoprecipitation-and-mass-spectrometry
#6
Walter van Dongen, Luc van Heerde, Sjef Boeren, Sacco C de Vries
A combination of coimmunoprecipitation (coIP) of tagged proteins followed by protein identification and quantitation using Liquid Chromatography Mass Spectrometry/Mass Spectrometry (LCMS/MS) has proven to be a reliable method to qualitatively characterize membrane-bound receptor complexes from plants. Success depends on a range of parameters, such as abundance and stability of the complex and functionality of the tagged receptors, efficiency of the protein complex isolation procedure, MS equipment, and analysis software in use...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28076774/chromatographic-efficiency-and-selectivity-in-top-down-proteomics-of-histones
#7
Yiyang Zhou, Ximo Zhang, Luca Fornelli, Philip D Compton, Neil Kelleher, Mary J Wirth
Histones are involved in epigenetic control of a wide variety of cellular processes through their multiple post-translational modifications. Their strongly cationic nature makes them challenging to separate with reversed-phase liquid chromatography coupled to mass spectrometry (RPLC-MS), where trifluoroacetic acid is avoided due to adduct formation. Columns with higher resolution are needed. In this work, RPLC-MS is performed on a histone sample using difluoroacetic acid and a 20-min gradient. Columns with C18 surfaces are compared for two different types of particle morphologies: 1) fully porous particles of 5μm in diameter, 2) superficially porous particles of 3μm in diameter with a shell of 0...
February 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28067053/global-profiling-of-metabolite-and-lipid-soluble-microbial-products-in-anaerobic-wastewater-reactor-supernatant-using-uplc-ms-e
#8
Phornpimon Tipthara, Chinagarn Kunacheva, Yan Ni Annie Soh, Stephen C C Wong, Ng Sean Pin, David C Stuckey, Bernhard O Boehm
Identification of soluble microbial products (SMPs) released during bacterial metabolism in mixed cultures in bioreactors is essential to understanding fundamental mechanisms of their biological production. SMPs constitute one of the main foulants (together with colloids and bacterial flocs) in membrane bioreactors widely used to treat and ultimately recycle wastewater. More importantly, the composition and origin of potentially toxic, carcinogenic, or mutagenic SMPs in renewable/reused water supplies must be determined and controlled...
January 25, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28063411/the-properties-of-red-seaweed-kappaphycus-alvarezii-and-its-effect-on-mammary-carcinogenesis
#9
Vi-Sion Chang, Patrick N Okechukwu, Swee-Sen Teo
The edible red seaweed (Kappaphycus alvarezii) is one of the algae species which was found to be rich in nutrients and nutraceutical. Hence, K. alvarezii may have the ability to suppress cancer through its antiproliferative properties. The aim of this study was to investigate the potential compounds of K. alvarezii, cytotoxicity properties of K. alvarezii extract on breast cancer cell line (MCF-7), investigated toxicity effect of high dosage K. alvarezii extract in rats and determined the effect of K. alvarezii on 7, 12-dimethylbenz[a]anthracene (DMBA) mammary carcinogenesis in rats...
January 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28061865/proteomic-analysis-of-enterotoxigenic-escherichia-coli-etec-in-neutral-and-alkaline-conditions
#10
Lucia Gonzales-Siles, Roger Karlsson, Diarmuid Kenny, Anders Karlsson, Åsa Sjöling
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in children and travelers to endemic areas. Secretion of the heat labile AB5 toxin (LT) is induced by alkaline conditions. In this study, we determined the surface proteome of ETEC exposed to alkaline conditions (pH 9) as compared to neutral conditions (pH 7) using a LPI Hexalane FlowCell combined with quantitative proteomics. Relative quantitation with isobaric labeling (TMT) was used to compare peptide abundance and their corresponding proteins in multiple samples at MS/MS level...
January 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28061410/photocatalytic-oxidation-of-trace-carbamazepine-in-aqueous-solution-by-visible-light-driven-znln2s4-performance-and-mechanism
#11
Longli Bo, Kebo He, Na Tan, Bo Gao, Qiqi Feng, Jiadong Liu, Lei Wang
In this work, visible-light-driven Znln2S4 photocatalyst (band gap 1.98 eV, absorption wavelength 300-600 nm) was synthesized by a facile hydrothermal method for photocatalytic degradation of trace pharmaceutical carbamazepine (CBZ) in aqueous solution, and characterized by UV-Vis diffusive reflectance spectroscopy, BET, SEM and XRD, respectively. It demonstrated that a complete degradation of CBZ was achieved with an optimal Znln2S4 dosage of 30 mg/L under a 100 W iodine-gallium lamp irradiation of 20 min, which afforded the reaction rate constant and half-life being respectively 10...
April 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28052759/exploring-the-use-of-routinely-available-retrospective-data-to-study-the-association-between-malaria-control-scale-up-and-micro-economic-outcomes-in-zambia
#12
Alison Comfort, Anthony Leegwater, Sharon Nakhimovsky, Henry Kansembe, Busiku Hamainza, Benson Bwalya, Martin Alilio, Ben Johns, Lauren Olsho
BACKGROUND: Country-level evidence on the impact of malaria control on micro-economic outcomes is vital for mobilizing domestic and donor resources for malaria control. Using routinely available survey data could facilitate this investigation in a cost-efficient way. METHODS: The authors used Malaria Indicator Surveys (MIS) and Living Conditions Monitoring Survey (LCMS) data from 2006 to 2010 for all 72 districts in Zambia to relate malaria control scale-up with household food spending (proxy for household well-being), educational attainment and agricultural production...
January 4, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28035802/rapid-distinction-of-leucine-and-isoleucine-in-monoclonal-antibodies-using-nanoflow-lcms-n
#13
Dhanashri Bagal, Eddie Kast, Ping Cao
Monoclonal antibodies (mAbs) are large heterogeneous molecules that represent a growing class of therapeutics. De novo sequencing of mAbs becomes necessary when the original cell line or the cDNA is unavailable. An important feature in sequencing of mAbs is the discrimination of isobaric residues (Xle): leucine (Leu) and isoleucine (Ile). An incorrect identification of the Xle site, especially in the complementarity determining regions (CDRs), can result in the production of an antibody with severely compromised efficacy...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28029204/translating-thermal-response-of-triblock-copolymer-assemblies-in-dilute-solution-to-macroscopic-gelation-and-phase-separation
#14
Zhe Sun, Ye Tian, Wendy L Hom, Oleg Gang, Surita R Bhatia, Robert B Grubbs
The thermal response of semi-dilute solutions (5 w/w%) of two amphiphilic thermoresponsive poly(ethylene oxide)-b-poly(N,N-diethylacrylamide)-b-poly(N,N-dibutylacrylamide) (PEO45 -PDEAmx -PDBAm12 ) triblock copolymers, which differ only in the size of the central responsive block, in water was examined. Aqueous PEO45 -PDEAm41 -PDBAm12 solutions, which undergo a thermally induced sphere-to-worm transition in dilute solution, were found to reversibly form soft (G'≈10 Pa) free-standing physical gels after 10 min at 55 °C...
December 28, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/28013465/decomposition-of-3-5-dinitrobenzamide-in-aqueous-solution-during-uv-h2o2-and-uv-tio2-oxidation-processes
#15
Yingjie Yan, Qi-Nan Liao, Feng Ji, Wei Wang, Shoujun Yuan, Zhen-Hu Hu
3,5-Dinitrobenzamide has been widely used as a feed additive to control coccidiosis in poultry, and part of the added 3,5-dinitrobenzamide is excreted into wastewater and surface water. The removal of 3,5-dinitrobenzamide from wastewater and surface water has not been reported in previous studies. Highly reactive hydroxyl radicals from UV/hydrogen peroxide (H2O2) and UV/titanium dioxide (TiO2) advanced oxidation processes (AOPs) can decompose organic contaminants efficiently. In this study, the decomposition of 3,5-dinitrobenzamide in aqueous solution during UV/H2O2 and UV/TiO2 oxidation processes was investigated...
December 24, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28013407/proteomic-based-biomarker-discovery-for-development-of-next-generation-diagnostics
#16
REVIEW
Akbar Khalilpour, Tugba Kilic, Saba Khalilpour, Mario Moisés Álvarez, Iman K Yazdi
In the post-genome age, proteomics is receiving significant attention because they provide an invaluable source of biological structures and functions at the protein level. The search for disease-specific biomarkers for diagnostic and/or therapeutic applications is one of the areas that proteomics is having a significant impact. Thus, the identification of a "good" biomarker enables a more accurate early diagnosis and prognosis of disease. Rapid advancements in mass spectrometry (MS) instrumentation, liquid chromatography MS (LCMS), protein microarray technology, and other protein profiling methodologies have a substantial expansion of our toolbox to identify disease-specific protein and peptide biomarkers...
January 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27984696/trapping-reactions-of-the-sulfenyl-and-sulfinyl-tautomers-of-sulfenic-acids
#17
Murugaeson R Kumar, Patrick J Farmer
Sulfenic acids react as both nucleophiles and electrophiles, which may be attributable to interconversion between sulfenyl and sulfinyl tautomers. We demonstrate one-pot trapping of both tautomeric forms of glutathione sulfenic acid by LCMS. The sulfinyl tautomers are characterized by reaction with nucleophilic reagents such as dimedone and cyanide, giving unique products that are analogous to corresponding adducts of aldehydes. Likewise, we show that aldehyde reactive reagents such as silyl enol ethers also react with glutathione sulfenic acid to give products characteristic of both sulfenyl and sulfinyl tautomers...
December 28, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27936436/spectroscopic-single-crystal-x-ray-hirshfeld-in-vitro-and-in-silico-biological-evaluation-of-a-new-series-of-potent-thiazole-nucleus-integrated-with-pyrazoline-scaffolds
#18
Vinutha V Salian, Badiadka Narayana, Balladka K Sarojini, Madan S Kumar, Govinahalli S Nagananda, Kullaiah Byrappa, Avinash K Kudva
In the present study, the spectroscopic characterization of a new series of substituted thiazole linked pyrazoline scaffolds 4a-l was performed. The formation of 4a-l from the intermediate 3-(4-chlorophenyl)-5-[4-(propan-2-yl)phenyl]-4,5-dihydro-1H-pyrazole-1-carbothioamide 2 and substituted 2-bromo-1-phenylethanone 3a-l was evidenced through the changes in FTIR, (1)H NMR, (13)C NMR, LCMS data. The X-ray diffraction studies revealed that compound 2 and 4g crystallized in monoclinic crystal system with P21/n space group...
March 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27925239/physiologically-based-pharmacokinetic-modeling-revealed-minimal-codeine-intestinal-metabolism-in-first-pass-removal-in-rats
#19
Keumhan Noh, Shu Chen, Qi J Yang, K Sandy Pang
The physiologically based model with segregated flow to the intestine (SFM-PBPK; partial, lower flow to enterocyte region vs. greater flow to serosal region) was found to describe the first-pass glucuronidation of morphine (M) to morphine-3β-glucuronide (MG) in rats after intraduodenal (i.d.) and intravenous (i.v.) administration better than the traditional model (TM), for which a single intestinal flow perfused the whole of the intestinal tissue. The segregated flow model (SFM) described a disproportionately greater extent of intestinal morphine glucuronidation for i...
January 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/27889674/design-synthesis-characterization-and-cation-sensing-behavior-of-amino-naphthoquinone-receptor-selective-colorimetric-sensing-of-cu-ii-ion-in-nearly-aqueous-solution-with-mimicking-logic-gate-operation
#20
C Parthiban, Kuppanagounder P Elango
An amino-naphthoquione receptor (R1) has been rationally designed, synthesized and characterized using (1)H and (13)C NMR, LCMS and single crystal X-ray diffraction studies. The receptor exhibits an instantaneous colour change from yellow to blue selectively with Cu(II) ions in water-DMF (98:2% v/v) medium. The results of UV-Vis and fluorescence spectral studies indicates that the mechanism of sensing involves formation of a 1:1 complex between R1 and Cu(II) ion. The proposed mechanism has been confirmed through product analysis using FT-IR, UV-Vis, EPR and HRMS studies in addition to magnetic moment and elemental analysis measurements...
March 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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