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https://www.readbyqxmd.com/read/28648005/-components-of-human-placental-extract-and-its-protective-effect-to-rat-from-d-galn-induced-acute-liver-injury
#1
H Q Lei, M L Cheng, J Li, P P Zhou, X H Luo, F Yu
Objective: To evaluate the effect of human placental extracts (HPE) on the protection from acute liver injury (ALI) induced by D-GalN and analyze the components of HPE. Methods: (1)Fourty male mice were randomly divided into five groups (Blank, Model, MgIG, HPE and HPE+ MgIG) for the ALI model and treatments.The serum alanine aminotransferase (ALT) and aspartate transaminase (AST) were determined by biochemical assays.Nitric monoxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) in serum and hepatic tissue were detected by assay kits...
June 20, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28644052/a-little-dab-will-do-ya-in-a-case-report-of-neuro-and-cardiotoxicity-following-use-of-cannabis-concentrates
#2
Shannon S Rickner, Dazhe Cao, Kurt Kleinschmidt, Steven Fleming
CONTEXT: The use of marijuana and cannabis concentrates is increasing, especially following decriminalization in several states. Psychosis and cardiotoxicity have been reported following cannabis use; however, myocardial injury from "dabbing" has not yet been reported. We report a case of hyperthermia, tachycardia, hypertension, severe agitation, neuro-, and cardiotoxicity following the use of "dabs" where there is concomitant confirmatory biological and sample testing. CASE DETAILS: A 17-year-old athletic man developed agitation requiring sedation and intubation for safety, with peak systolic blood pressures in the 190s and hyperthermia (to 102 °F)...
June 23, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28643405/reassessment-of-the-access-testosterone-chemiluminescence-assay-and-comparison-with-lc-ms-method
#3
Ruggero Dittadi, Mara Matteucci, Elisa Meneghetti, Rudina Ndreu
AIM OF THE STUDY: To reassess the imprecision and Limit of Quantitation, to evaluate the cross-reaction with dehydroepiandrosterone-sulfate (DHEAS), the accuracy toward liquid chromatography-mass spectrometry (LC-MS) and the reference interval of the Access Testosterone method, performed by DxI immunoassay platform (Beckman Coulter). MATERIAL AND METHODS: Imprecision was evaluated testing six pool samples assayed in 20 different run using two reagents lots. The cross-reaction with DHEAS was studied both by a displacement curve and by spiking DHEAS standard in two serum samples with known amount of testosterone...
June 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28640638/enzyme-mediated-conversion-of-fad-to-8-formyl-fad-in-formate-oxidase-results-in-modified-cofactor-with-enhanced-catalytic-properties
#4
John M Robbins, Michael G Souffrant, Donald Hamelberg, Giovanni Gadda, Andreas S Bommarius
Flavins, including flavin adenine dinucleotide (FAD), are fundamental catalytic cofactors responsible for the redox functionality of a diverse set of proteins. Alternatively, modified flavin analogues are rarely found in nature as their incorporation typically results in inactivation of flavoproteins, thus leading to the disruption of important cellular pathways. Here, we report that the fungal flavoenzyme formate oxidase (FOX) catalyzes the slow conversion of non-covalently bound FAD to 8-formyl FAD (8-fFAD), and that this conversion results in a nearly 10-fold increase in formate oxidase activity...
June 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28640347/psychotria-viridis-chemical-constituents-from-leaves-and-biological-properties
#5
Débora B S Soares, Lucienir P Duarte, André D Cavalcanti, Fernando C Silva, Ariadne D Braga, Miriam T P Lopes, Jacqueline A Takahashi, Sidney A Vieira-Filho
The phytochemical study of hexane, chloroform and methanol extracts from leaves of Psychotria viridis resulted in the identification of: the pentacyclic triterpenes, ursolic and oleanolic acid; the steroids, 24-methylene-cycloartanol, stigmasterol and β-sitosterol; the glycosylated steroids 3-O-β-D-glucosyl-β-sitosterol and 3-O-β-D-glucosyl-stigmasterol; a polyunsaturated triterpene, squalene; the esters of glycerol, 1-palmitoylglycerol and triacylglycerol; a mixture of long chain hydrocarbons; the aldehyde nonacosanal; the long chain fat acids hentriacontanoic, hexadecanoic and heptadenoic acid; the ester methyl heptadecanoate; the 4-methyl-epi-quinate and two indole alkaloids, N,N-dimethyltryptamine (DMT) and N-methyltryptamine...
April 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28612376/first-report-on-a-novel-nigrospora-sphaerica-isolated-from-catharanthus-roseus-plant-with-anticarcinogenic-properties
#6
Farah Wahida Ayob, Khanom Simarani, Nurhayati Zainal Abidin, Jamaludin Mohamad
This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography-mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA-MB 231) was treated with a purified vinblastine which was intracellulary produced by N...
July 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28605582/assessing-antibiotic-permeability-of-gram-negative-bacteria-via-nanofluidics
#7
SoonGweon Hong, Tobias J Moritz, Christopher M Rath, Pramila Tamrakar, Philip Lee, Thomas Krucker, Luke P Lee
While antibiotic resistance is increasing rapidly, drug discovery has proven to be extremely difficult. Antibiotic resistance transforms some bacterial infections into deadly medical conditions. A significant challenge in antibiotic discovery is designing potent molecules that enter Gram-negative bacteria and also avoid active efflux mechanisms. Critical analysis in rational drug design has been hindered by the lack of effective analytical tools to analyze the bacterial membrane permeability of small molecules...
June 16, 2017: ACS Nano
https://www.readbyqxmd.com/read/28599879/uncommon-toxic-microbial-metabolite-patterns-in-traditionally-home-processed-maize-dish-fufu-consumed-in-rural-cameroon
#8
Wilfred A Abia, Benedikt Warth, Chibundu N Ezekiel, Bojan Sarkanj, Paul C Turner, Doris Marko, Rudolf Krska, Michael Sulyok
Toxins of microbial origin frequently contaminate foodstuffs worldwide and pose a serious hazard to humans. This study reports on LCMS/MS quantification of multiple fungal and bacterial toxins, from household sampling of 50 traditionally prepared maize-fufu samples from Bamunka village, western highlands of Cameroon. Seventy-four metabolites including aflatoxin B1 (AFB1) (12/50: mean 0.9, range n.d-1.8 μg kg(-1)), cereulide (50/50: mean 37; range 1-236 μg kg(-1)), deoxynivalenol (DON) (50/50: mean 23, range 14-55 μg kg(-1)), fumonisin B1 (FB1) (50/50: mean: 151, range 48-709 μg kg(-1)), nivalenol (NIV) (50/50; mean 268, range 116-372 μg kg(-1)), patulin (PAT) (15/50:mean 105, range 12-890 μg kg(-1)) and zearalenone (ZEN) (50/50: mean 49, range 5-150) were detected; and of note every sample contained at least 27 toxic compounds...
June 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28578903/intrinsic-capabilities-of-leptospermum-javanicum-in-inducing-apoptosis-and-suppressing-the-metastatic-potential-of-human-lung-carcinoma-cells
#9
Suerialoasan Navanesan, Norhanom Abdul Wahab, Sugumaran Manickam, Yuen Lin Cheow, Kae Shin Sim
The active isolate of LF1 in Leptospermum javanicum was further looked into its capabilities in provoking an apoptotic reaction and suppressing the metastasis process in treated non-small lung cancer cells. LF1 underwent isolation and purification to yield a white powder which was identified as Betulinic acid (BA) via NMR, LCMS and IR spectroscopy. The isolate, BA, which produced an encouraging cytotoxic effect against non-small lung cancer cells (A549 and NCI-H1299) through the MTT assay, was further assessed with TUNEL, Sub-G1 population quantification, acridine orange/ethidium bromide staining as well as activated caspase-3 detection...
June 2, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28561766/genotypic-variation-in-wheat-flour-lysophospholipids
#10
Lei Liu, Qi Guo, Zhonghu He, Xianchun Xia, Daniel L E Water, Carolyn A Raymond, Graham J King
Lysophospholipids (LPLs) are the most abundant polar lipids in wheat endosperm and naturally complex with amylose, affecting starch physicochemical properties. We analyzed LPLs in wheat flour from 58 cultivars which differ by grain hardness using liquid chromatography mass spectrometry (LCMS). There were significant differences in LPL content between cultivars, demonstrating that genotype rather than environment contributes most to the total variance in wheat endosperm LPLs. Polar lipids such as LPLs may play a role in grain hardness through their interaction with puroindoline proteins, however, no strong correlation between kernel hardness and LPLs was detected...
May 31, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28520799/nonenzymatic-glycosylation-of-human-serum-albumin-and-its-effect-on-antibodies-profile-in-patients-with-diabetes-mellitus
#11
Alok Raghav, Jamal Ahmad, Khursheed Alam
BACKGROUND: Albumin glycation and subsequent formation of advanced glycation end products (AGEs) correlate with diabetes and associated complications. METHODS: Human Serum Albumin (HSA) was modified with D-glucose for a 40 day period under sterile conditions at 37°C. Modified samples along with native HSA (unmodified) were analyzed for structural modifications by UV and fluorescence, FTIR, Liquid chromatography mass spectrometry (LCMS) and X-ray crystallography...
2017: PloS One
https://www.readbyqxmd.com/read/28513638/automatic-assignment-of-metal-containing-peptides-in-proteomic-lc-ms-and-ms-ms-data-sets
#12
Christopher A Wootton, Yuko P Y Lam, Matthew Willetts, Maria A van Agthoven, Mark P Barrow, Peter J Sadler, Peter B O Connor
Transition metal-containing proteins and enzymes are critical for the maintenance of cellular function and metal-based (metallo)drugs are commonly used for the treatment of many diseases, such as cancer. Detection and characterisation of metallodrug targets is crucial for improving drug-design and therapeutic efficacy. Due to the unique isotopic ratios of many metal species, and the complexity of proteomic samples, standard MS data analysis of these species is unsuitable for accurate assignment. Herein a new method for differentiating metal-containing species within complex LCMS data is presented based upon the Smart Numerical Annotation Procedure (SNAP)...
May 17, 2017: Analyst
https://www.readbyqxmd.com/read/28505491/development-of-suitable-method-for-large-scale-urinary-glucocorticoid-analysis-by-liquid-chromatography-mass-spectrometry
#13
Akiko Matsumoto, Chisato Shimanoe, Keitaro Tanaka, Masayoshi Ichiba, Megumi Hara
Levels of urinary glucocorticoids and their concentration ratios have been analyzed as potential markers for various pathological statuses. Large-scale studies may possibly accelerate the investigations; however, a suitable method needs to be established. Analytical conditions for measurement of urinary glucocorticoids with LCMS were examined. Electrospray ionization in the positive ion mode was applied for detection of cortisol (precursor>product ion: 363.3>121.0), cortisol-d4 (internal standard, IS, 367...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28496489/homoisoflavonoids-and-the-antioxidant-activity-of-ophiopogon-japonicus-root
#14
Yancui Wang, Feng Liu, Zongsuo Liang, Liang Peng, Bangqing Wang, Jing Yu, Yingying Su, Cunde Ma
The root of Ophiopogon japonicus has been used as a traditional Chinese medicine and also a functional food ingredient for a long time in China. In the present study, 17 different homoisoflavonoid compounds were identified in the root extract of O. japonicus by HPLC-DAD and LCMS/MS analyses. The antioxidant activity of the of chloroform/methanol (1:1, v/v), methanol and 70% ethanol extracts, and two major isolated homoisoflavonoid compounds (methylophiopogonanone A and methylophiopogonanone B) from O. japonicus root were investigated by various in-vitro assays...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28494649/herbicidal-activity-of-pure-compound-isolated-from-rhizosphere-inhabiting-aspergillus-flavus
#15
Saeed Ullah Khattak, Ghosia Lutfullah, Zafar Iqbal, Irshad Ur Rehman, Jamshaid Ahmad, Abid Ali Khan
In the quest for bioactive natural products of fungal origin, Aspergillus flavus was isolated from rhizosphere of Mentha piperita using Potato Dextrose Agar (PDA) and Czapec Yeast Broth (CYB) nutrient media for metabolites production. In total, three different metabolites were purified using HPLC/LCMS and the structures were established using 500 Varian NMR experiments. Further the isolated metabolites in different concentrations (10, 100, 1000 μg/mL) were tested for herbicidal activity using Completely Randomized design (CRD) against the seeds of Silybum marianum and Avena fatua which are major threats to wheat crop in Pakistan...
May 11, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28490120/evaluation-of-antioxidant-potential-of-artocarpus-heterophyllus-l-j33-variety-fruit-waste-from-different-extraction-methods-and-identification-of-phenolic-constituents-by-lcms
#16
Mohd Nazrul Hisham Daud, Dian Nashiela Fatanah, Noriham Abdullah, Rohaya Ahmad
Artocarpus heterophyllus J33 (AhJ33) fruit is a popular and valuable jackfruit variety in Malaysia. For export, the pulp has to be separated from the skin which is usually discarded. Hence, the conversion of the fruit waste to food products with economic value needs to be explored utilizing the waste to wealth concept. This paper reports the evaluation of antioxidant potential of AhJ33 fruit waste (rind and rachis) extracts from three different extraction methods (maceration, percolation and Soxhlet). The antioxidant potential was assessed by DPPH radical scavenging, FRAP and β-carotene bleaching assays...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28445069/targeted-dereplication-of-microbial-natural-products-by-high-resolution-ms-and-predicted-lc-retention-time
#17
Justine Chervin, Marc Stierhof, Ming Him Tong, Doe Peace, Kine Østnes Hansen, Dagmar Solveig Urgast, Jeanette Hammer Andersen, Yi Yu, Rainer Ebel, Kwaku Kyeremeh, Veronica Paget, Gabriela Cimpan, Albert Van Wyk, Hai Deng, Marcel Jaspars, Jioji N Tabudravu
A new strategy for the identification of known compounds in Streptomyces extracts that can be applied in the discovery of natural products is presented. The strategy incorporates screening a database of 5555 natural products including 5098 structures from Streptomyces sp., using a high-throughput LCMS data processing algorithm that utilizes HRMS data and predicted LC retention times (tR) as filters for rapid identification of known compounds in the natural product extract. The database, named StrepDB, contains for each compound the structure, molecular formula, molecular mass, and predicted LC retention time...
April 26, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28441352/high-throughput-determination-of-sodium-danshensu-in-beagle-dogs-by-the-lcms-ms-method-employing-liquid-liquid-extraction-based-on-96-well-format-plates
#18
Jingjing Jiang, Xin Zhao, Xiuxiu Li, Shengyuan Wu, Shidan Yu, Yuefen Lou, Guorong Fan
Sodium Danshensu (sodium d-(+)-β-(3,4-dihydroxyphenyl) lactate), one of the water-soluble ingredients in Salvia miltiorrhiza, exhibits potent relaxation of the coronary artery and anticoagulation effection. A high-throughput, rapid, and sensitive method combining liquid chromatography with electrospray ionization tandem mass spectrometry to determine the sodium danshensu in beagle dog plasma was developed and validated, using gallic acid as an internal standard (IS). Acidified plasma samples were extracted using 96-well liquid-liquid extraction, and were eluted on a CNW Athena C18 column (3 μm, 2...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28433718/three-new-piscidins-from-orange-spotted-grouper-epinephelus-coioides-phylogeny-expression-and-functional-characterization
#19
Ze-Rui Zhuang, Xiao-Dong Yang, Xia-Zi Huang, Hui-Xian Gu, Han-Yin Wei, Yi-Jun He, Li Deng
The present study reports the identification, and characterization of three new putative piscidin paralogues, ecPis-2, ecPis-3 and ecPis-4, from orange-spotted grouper (Epinephelus coioides). The cDNA of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. The phylogenetic analysis indicated that multiple piscidin paralogues in one fish species are highly diversified, the analysis suggested that the piscidins should be a family belonging to the superfamily of ancient cationic, linear, and amphipathic host defence peptides widespread across invertebrate and vertebrate taxa comprising insect cecropins and ceratotoxins, and the amphibian dermaseptins...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28424496/the-chemical-characterization-of-nigerian-propolis-samples-and-their-activity-against-trypanosoma-brucei
#20
Ruwida Omar, John O Igoli, Tong Zhang, Alexander I Gray, Godwin U Ebiloma, Carol J Clements, James Fearnley, RuAngeli Edrada Ebel, Tim Paget, Harry P de Koning, David G Watson
Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b...
April 19, 2017: Scientific Reports
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