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Metabolomic analysis

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https://www.readbyqxmd.com/read/28319722/bisphenol-s-exposure-modulate-macrophage-phenotype-as-defined-by-cytokines-profiling-global-metabolomics-and-lipidomics-analysis
#1
Chao Zhao, Zhi Tang, Jiacheng Yan, Jing Fang, Hailin Wang, Zongwei Cai
As an important structural analogue of bisphenol A (BPA), bisphenol S (BPS) has been used as alternatives to BPA in industrialized production. However, the immunotoxicity of BPS remains poorly understood. As a critical model in inflammatory responses, macrophages are used to explore the immunotoxic potential and mechanisms of BPS at environmentally relevant concentrations in our study. Here, we are combining molecular toxicology and mass spectrometry (MS)-based global metabolomics and lipidomics study together to estimate the variation of cytokines profiling and metabolism characteristic following BPS exposure...
March 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28317741/a-novel-method-for-the-quality-control-of-saffron-through-the-simultaneous-analysis-of-authenticity-and-adulteration-markers-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#2
Miguel Guijarro-Díez, María Castro-Puyana, Antonio Luis Crego, María Luisa Marina
A liquid chromatography-(quadrupole-time of flight)-mass spectrometry methodology was developed to assess the authenticity of saffron through the analysis of a group of kaempferol derivatives recently proposed as novel authenticity markers as a result of a metabolomic study of saffron (kaempferol 3-O-glucoside, kaempferol 3-O-sophoroside, kaempferol 3,7-O-diglucoside, kaempferol 3,7,4'-O-triglucoside, kaempferol 3-O-sophoroside-7-O-glucoside). Geniposide was also studied as an adulteration marker of saffron with gardenia...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317248/influence-of-different-processing-times-on-the-quality-of-polygoni-multiflora-radix-by-metabolomics-based-on-ultra-high-performance-liquid-chromatography-with-quadrupole-time-of-flight-mass-spectrometry
#3
Xie-An Yu, Ai-Hua Ge, Lu Zhang, Jin Li, Mingrui An, Jun Cao, Jun He, Xiu-Mei Gao, Yan-Xu Chang
A metabolomics method based on ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry was developed to evaluate the influence of processing times on the quality of raw and processed Polygoni Multiflora Radix. Principal component analysis and partial least squares discriminant analysis was used to screen the potential maker metabolites that were contributed to the quality changes. Then these marker metabolites were selected as variables in Fisher's discriminant analysis to establish the models that were used to distinguish the raw and processed Polygoni Multiflora Radix in the markets...
March 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28315793/highlighting-the-effects-of-coumarin-on-adult-plants-of-arabidopsis-thaliana-l-heynh-by-an-integrated-omic-approach
#4
Fabrizio Araniti, Monica Scognamiglio, Angela Chambery, Rosita Russo, Assunta Esposito, Brigida D'Abrosca, Antonio Fiorentino, Antonio Lupini, Francesco Sunseri, Maria Rosa Abenavoli
In this study, the effects of the allelochemical coumarin through a metabolomic, proteomic and morpho-physiological approach in Arabidopsis adult plants (25days old) were investigated. Metabolomic analysis evidenced an increment of amino acids and a high accumulation of soluble sugars, after 6days of coumarin treatment. This effect was accompanied by a strong decrease on plant fresh and dry weights, as well as on total protein content. On the contrary, coumarin did not affect leaf number but caused a reduction in leaf area...
March 1, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28315235/identification-and-quantitation-of-malonic-acid-biomarkers-of-in-born-error-metabolism-by-targeted-metabolomics
#5
Chandra Shekar R Ambati, Furong Yuan, Lutfi A Abu-Elheiga, Yiqing Zhang, Vivekananda Shetty
Malonic acid (MA), methylmalonic acid (MMA), and ethylmalonic acid (EMA) metabolites are implicated in various non-cancer disorders that are associated with inborn-error metabolism. In this study, we have slightly modified the published 3-nitrophenylhydrazine (3NPH) derivatization method and applied it to derivatize MA, MMA, and EMA to their hydrazone derivatives, which were amenable for liquid chromatography- mass spectrometry (LC-MS) quantitation. 3NPH was used to derivatize MA, MMA, and EMA, and multiple reaction monitoring (MRM) transitions of the corresponding derivatives were determined by product-ion experiments...
March 17, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28315126/screening-for-main-components-associated-with-the-idiosyncratic-hepatotoxicity-of-a-tonic-herb-polygonum-multiflorum
#6
Chunyu Li, Ming Niu, Zhaofang Bai, Congen Zhang, Yanling Zhao, Ruiyu Li, Can Tu, Huifang Li, Jing Jing, Yakun Meng, Zhijie Ma, Wuwen Feng, Jinfa Tang, Yun Zhu, Jinjie Li, Xiaoya Shang, Zhengsheng Zou, Xiaohe Xiao, Jiabo Wang
The main constituents of a typical medicinal herb, Polygonum multiflorum (Heshouwu in Chinese), that induces idiosyncratic liver injury remain unclear. Our previous work has shown that cotreatment with a nontoxic dose of lipopolysaccharide (LPS) and therapeutic dose of Heshouwu can induce liver injury in rats, whereas the solo treatment cannot induce observable injury. In the present work, using the constituent "knock-out" and "knock-in" strategy, we found that the ethyl acetate (EA) extract of Heshouwu displayed comparable idiosyncratic hepatotoxicity to the whole extract in LPS-treated rats...
March 17, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28314999/genetic-diversity-and-metabolic-profile-of-salvia-officinalis-populations-implications-for-advanced-breeding-strategies
#7
Eirini Sarrou, Ioannis Ganopoulos, Aliki Xanthopoulou, Domenico Masuero, Stefan Martens, Panagiotis Madesis, Athanasios Mavromatis, Paschalina Chatzopoulou
As a result of this work, we were able to characterize seven indigenous to Greece Salvia officinalis populations using genetic and metabolomic tools. These tools can be used to select the most promising genotypes, capable to design future breeding programs for high valuable varieties. An initial investigation was carried out to compare the genetic and metabolic diversity in S. officinalis grown in Greece and to discern the relationship between the two sets of data. Analysis of inter-simple sequence repeats (ISSR) revealed significant genetic differences among seven sage populations, which were grouped into three main clusters according to an UPGMA ISSR data-based dendrogram and Principle Coordinate Analysis...
March 17, 2017: Planta
https://www.readbyqxmd.com/read/28314628/petit-high-pressure-carbon-dioxide-stress-increases-synthesis-of-s-adenosylmethionine-and-phosphatidylcholine-in-yeast-saccharomyces-cerevisiae
#8
Liyuan Niu, Kazuki Nomura, Hitoshi Iwahashi, Hiroyuki Matsuoka, Satoshi Kawachi, Yoshihisa Suzuki, Katsuhiro Tamura
Petit-High Pressure Carbon Dioxide (p-HPCD) is a promising nonthermal technology for foods pasteurization. Cluster analysis of gene expression profiles of Saccharomyces cerevisiae exposed to various stresses exhibited that gene expression profile for p-HPCD stress (0.5MPa, 25°C) was grouped into a cluster including profiles for Sodium Dodecyl Sulfate and Roundup herbicide. Both are detergents that can disorder membrane structurally and functionally, which suggests that cell membrane may be a target of p-HPCD stress to cause cell growth inhibition...
March 8, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28314466/gc-ms-based-gestational-diabetes-mellitus-longitudinal-study-identification-of-2-and-3-hydroxybutyrate-as-potential-prognostic-biomarkers
#9
Danuta Dudzik, Marcin Zorawski, Mariusz Skotnicki, Wieslaw Zarzycki, Antonia García, Santiago Angulo, M Paz Lorenzo, Coral Barbas, M Pilar Ramos
Gestational Diabetes Mellitus (GDM) causes severe short- and long-term complications for the mother, fetus and neonate, including type 2-diabetes (T2DM) later in life. In this pilot study, GC-Q/MS analysis was applied for plasma metabolomics fingerprinting of 24 healthy and 24 women with GDM at different stages of gestation (second and third trimester) and postpartum (one and three months). Multivariate (unsupervised and supervised) statistical analysis was performed to investigate variance in the data, identify outliers and for unbiased assessment of data quality...
March 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28314371/intrauterine-growth-restriction-programs-the-hypothalamus-of-adult-male-rats-integrated-analysis-of-proteomic-and-metabolomic-data
#10
Amanda Paula Pedroso, Adriana Pereira de Souza, Ana Paula Segantine Dornellas, Lila Missae Oyama, Cláudia Maria Oller Nascimento, Gianni Mara Silva Santos, José C Rosa, Ricardo Pimenta Bertolla, Jelena Klawitter, Uwe Christians, Alexandre Keiji Tashima, Eliane Beraldi Ribeiro
Programming of hypothalamic functions regulating energy homeostasis may play a role in intrauterine growth restriction (IUGR)-induced adulthood obesity. The present study investigated the effects of IUGR on the hypothalamus proteome and metabolome of adult rats submitted to 50% protein-energy restriction throughout pregnancy. Proteomic and metabolomic analyzes were performed by data independent acquisition mass spectrometry and multiple reaction monitoring, respectively. At age 4 months, the restricted rats showed elevated adiposity, increased leptin and signs of insulin resistance...
March 17, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28314215/a-metabolomic-approach-shows-sphingosine-1-phosphate-and-lysophospholipids-as-mediators-of-the-therapeutic-effect-of-liver-growth-factor-in-emphysema
#11
A Navarrete, F J Rupérez, T O Mendes, S Pérez-Rial, A Girón-Martínez, R Terrón-Expósito, J J Díaz-Gil, G Peces-Barba, C Barbas, A García
Tobacco smoke exposure is the principal cause of lung tissue destruction, which in turn results in emphysema that leads into shortness of breath. Liver growth factor (LGF, a cell and tissue regenerating factor with therapeutic activity in several organs) has antifibrotic and antioxidant properties that could be useful to promote lung tissue regenerating capacity in damaged lungs. The current study has examined differences in metabolite profiles (fingerprints) of plasma from mice (strain C57BL/6J, susceptible to develop emphysema) exposed to tobacco smoke during six months...
February 27, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28306342/nmr-based-plasma-metabolomic-discrimination-for-male-fertility-assessment-of-rats-treated-with-eurycoma-longifolia-extracts
#12
Forough Ebrahimi, Baharudin Ibrahim, Chin-Hoe Teh, Vikneswaran Murugaiyah, Kit-Lam Chan
Male infertility is one of the leading causes of infertility which affects many couples worldwide. Semen analysis is a routine examination of male fertility status which is usually performed on semen samples obtained through masturbation that may be inconvenient to patients. Eurycoma longifolia (Tongkat Ali, TA), native to Malaysia, has been traditionally used as a remedy to boost male fertility. In our recent studies in rats, upon the administration of high-quassinoid content extracts of TA including TA water (TAW), quassinoid-rich TA (TAQR) extracts, and a low-quassinoid content extract including quassinoid-poor TA (TAQP) extract, sperm count (SC) increased in TAW- and TAQR-treated rats when compared to the TAQP-treated and control groups...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306241/development-of-chemical-isotope-labeling-lc-ms-for-milk-metabolomics-comprehensive-and-quantitative-profiling-of-the-amine-phenol-submetabolome
#13
Dorothea Mung, Liang Li
Milk is a complex sample containing a variety of proteins, lipids and metabolites. Studying the milk metabolome represents an important application of metabolomics in the general area of nutritional research. However, comprehensive and quantitative analysis of milk metabolites is a challenging task due to the wide range of variations in chemical/physical properties and concentrations of these metabolites. We report an analytical workflow for in-depth profiling of the milk metabolome based on chemical isotope labeling (CIL) and liquid chromatography mass spectrometry (LC-MS) with a focus of using dansylation labeling to target the amine/phenol submetabolome...
March 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28302489/modelling-urea-cycle-disorder-citrullinemia-type-1-with-disease-specific-ipscs
#14
Elena Yukie Yoshitoshi-Uebayashi, Taro Toyoda, Katsutaro Yasuda, Maki Kotaka, Keiko Nomoto, Keisuke Okita, Kentaro Yasuchika, Shinya Okamoto, Noriyuki Takubo, Toshiya Nishikubo, Tomoyoshi Soga, Shinji Uemoto, Kenji Osafune
Citrullinemia type 1 (CTLN1) is a urea cycle disorder (UCD) caused by mutations of the ASS1 gene, which is responsible for production of the enzyme argininosuccinate synthetase (ASS), and classically presented as life-threatening hyperammonemia in newborns. Therapeutic options are limited, and neurological sequelae may persist. To understand the pathophysiology and find novel treatments, induced pluripotent stem cells (iPSCs) were generated from a CTLN1 patient and differentiated into hepatocyte-like cells (HLCs)...
March 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28301071/applications-of-high-resolution-magic-angle-spinning-mrs-in-biomedical-studies-i-cell-line-and-animal-models
#15
REVIEW
Eva Kaebisch, Taylor L Fuss, Lindsey A Vandergrift, Karin Toews, Piet Habbel, Leo L Cheng
High-resolution magic angle spinning (HRMAS) MRS allows for direct measurements of non-liquid tissue and cell specimens to present valuable insights into the cellular metabolisms of physiological and pathological processes. HRMAS produces high-resolution spectra comparable to those obtained from solutions of specimen extracts but without complex metabolite extraction processes, and preserves the tissue cellular structure in a form suitable for pathological examinations following spectroscopic analysis. The technique has been applied in a wide variety of biomedical and biochemical studies and become one of the major platforms of metabolomic studies...
March 16, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28300637/integrated-mass-spectrometry-imaging-and-omics-workflows-on-the-same-tissue-section-using-grid-aided-parafilm-assisted-microdissection
#16
Jusal Quanico, Julien Franck, Maxence Wisztorski, Michel Salzet, Isabelle Fournier
BACKGROUND: In spite of the number of applications describing the use of MALDI MSI, one of its major drawbacks is the limited capability of identifying multiple compound classes directly on the same tissue section. METHODS: We demonstrate the use of grid-aided, parafilm-assisted microdissection to perform MALDI MS imaging and shotgun proteomics and metabolomics in a combined workflow and using only a single tissue section. The grid is generated by microspotting acid dye 25 using a piezoelectric microspotter, and this grid was used as a guide to locate regions of interest and as an aid during manual microdissection...
March 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28300220/effects-of-gliadin-consumption-on-the-intestinal-microbiota-and-metabolic-homeostasis-in-mice-fed-a-high-fat-diet
#17
Li Zhang, Daniel Andersen, Henrik Munch Roager, Martin Iain Bahl, Camilla Hartmann Friis Hansen, Niels Banhos Danneskiold-Samsøe, Karsten Kristiansen, Ilinca Daria Radulescu, Christian Sina, Henrik Lauritz Frandsen, Axel Kornerup Hansen, Susanne Brix, Lars I Hellgren, Tine Rask Licht
Dietary gluten causes severe disorders like celiac disease in gluten-intolerant humans. However, currently understanding of its impact in tolerant individuals is limited. Our objective was to test whether gliadin, one of the detrimental parts of gluten, would impact the metabolic effects of an obesogenic diet. Mice were fed either a defined high-fat diet (HFD) containing 4% gliadin (n = 20), or a gliadin-free, isocaloric HFD (n = 20) for 23 weeks. Combined analysis of several parameters including insulin resistance, histology of liver and adipose tissue, intestinal microbiota in three gut compartments, gut barrier function, gene expression, urinary metabolites and immune profiles in intestinal, lymphoid, liver and adipose tissues was performed...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300194/amniotic-fluid-metabolomics-and-biochemistry-analysis-provides-novel-insights-into-the-diet-regulated-foetal-growth-in-a-pig-model
#18
Jin Wan, Fei Jiang, Jiao Zhang, Qingsong Xu, Daiwen Chen, Bing Yu, Xiangbing Mao, Jie Yu, Yuheng Luo, Jun He
Foetal loss and intrauterine growth restriction are major problems in mammals, but there are few effective ways in preventing it. Intriguingly, chitosan oligosaccharide (COS), a biomaterial derived from chitosan, can promote foetal survival and growth. Therefore, we have investigated how COS affects foetal survival and growth in a pig model. Fifty-two sows were divided into two treatment groups (n = 26) and fed either solely a control diet or a control diet that includes 100 mg/kg COS. Amniotic fluid and foetus samples from six sows that were of average body weight in each group were collected on gestation day 35...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300186/renal-medulla-is-more-sensitive-to-cisplatin-than-cortex-revealed-by-untargeted-mass-spectrometry-based-metabolomics-in-rats
#19
Pei Zhang, Jia-Qing Chen, Wan-Qiu Huang, Wei Li, Yin Huang, Zun-Jian Zhang, Feng-Guo Xu
Nephrotoxicity has long been the most severe and life-threatening side-effect of cisplatin, whose anticancer effect is therefore restricted. Previous pathological studies have shown that both renal cortex and medulla could be injured by cisplatin. Our TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay results further uncovered that medulla subjected more severe injury than cortex. In order to depict the underlying metabolic mechanism of spatial difference in response to cisplatin, in the present study, mass spectrometry-based untargeted metabolomics approach was applied to profile renal cortex and medulla metabolites of rat after receiving a single dose of cisplatin (2...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299975/targeted-high-performance-liquid-chromatography-tandem-mass-spectrometry-based-metabolomics-differentiates-metabolic-syndrome-from-obesity
#20
Fanyi Zhong, Mengyang Xu, Richard S Bruno, Kevin D Ballard, Jiangjiang Zhu
Both obesity and the metabolic syndrome are risk factors for type 2 diabetes and cardiovascular disease. Identification of novel biomarkers are needed to distinguish metabolic syndrome from equally obese individuals in order to direct them to early interventions that reduce their risk of developing further health problems. We utilized mass spectrometry-based targeted metabolic profiling of 221 metabolites to evaluate the associations between metabolite profiles and established metabolic syndrome criteria (i...
April 2017: Experimental Biology and Medicine
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