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Metalloproteinase 9

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https://www.readbyqxmd.com/read/29346149/testing-elevated-protease-activity-prospective-analysis-of-160-wounds
#1
Anike Lockmann, Tillmann Schill, Franziska Hartmann, Lisa-Lena Grönemeyer, Ricarda Holzkamp, Michael P Schön, Kai-Martin Thoms
OBJECTIVE: Given that local elevated protease activity (EPA) has been implicated in impaired wound healing, a prospective single-center study was conducted to assess protease activity in various wound types. METHODS: Protease activity was determined using an easy-to-use test system (Woundchek Protease Status Test Kit; Systagenix, Gatwick, United Kingdom) in 160 wounds in 143 patients. The assay detects the combined activity of inflammatory proteases, mainly matrix metalloproteinases 8 and 9 and human neutrophil elastase...
February 2018: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29344966/quorum-sensing-molecule-dihydroxy-2-3-pentanedione-and-its-analogs-as-regulators-of-epithelial-integrity
#2
S Elmanfi, X Ma, H O Sintim, E Könönen, S Syrjänen, U K Gursoy
BACKGROUND AND OBJECTIVE: Quorum-sensing molecules regulate the behavior of bacteria within biofilms and at the same time elicit an immune response in host tissues. Our aim was to investigate the regulatory role of dihydroxy-2,3-pentanedione (DPD), the precursor of universal autoinducer-2 (AI-2), and its analogs (ethyl-DPD, butyl-DPD and isobutyl-DPD) in the integrity of gingival epithelial cells. MATERIAL AND METHODS: Human gingival keratinocytes were incubated with four concentrations (10 μmol L-1 , 1 μmol L-1 , 100 nmol L-1 and 10 nmol L-1 ) of DPD and its analogs for 24 hours...
January 17, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29344659/degree-of-endplate-chondrocyte-degeneration-in-different-tension-regions-during-mechanical-stimulation
#3
Yongming Xu, Shujuan Xu, Zhi Gao, Liang Xiao, Fei Xiao, Hongguang Xu, Xiaoling Zhang
The aim of this study was to explore the degree of degeneration of endplate chondrocytes in different tension regions induced by intermittent cyclic mechanical tension (ICMT) in vitro. Rat endplate chondrocytes were harvested and treated with 10% ICMT for 8 h/day with a frequency of 0.5 Hz. A cartilage degeneration model was induced using an FX‑5000T cell strain‑loading system. The experiment was divided into the central region and the peripheral region, according to the contact area between the loading post and the six‑well flexible silicone rubber BioFlex plates...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29344645/targeted-disruption-of-adenosine-kinase-in-myeloid-monocyte-cells-increases-osteoclastogenesis-and-bone-resorption-in-mice
#4
Qiuying Ye, Ge Li, Shuhua Liu, Yalun Guan, Yunfeng Li, Jinling Li, Huanhuan Jia, Xuejiao Li, Qingnan Li, Ren Huang, Hui Wang, Yu Zhang
Adenosine kinase (ADK) serves an important role in intracellular adenosine clearance via phosphorylating adenosine to AMP. The role of adenosine and its receptors in the maintenance of bone homeostasis is well studied, particularly in osteoclastogenesis and bone resorption; however, the function of ADK in bone metabolism is still unclear. In the present study, utilizing the cre/floxp recombination system, mice with conditional loss of ADK function in myeloid monocyte cells were used to assess the effect of ADK deficiency on bone metabolism...
January 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29344204/silibinin-inhibits-the-migration-and-invasion-of-human-gastric-cancer-sgc7901-cells-by-downregulating-mmp-2-and-mmp-9-expression-via-the-p38mapk-signaling-pathway
#5
Shuming Lu, Zhuqing Zhang, Meiru Chen, Chunyan Li, Lina Liu, Yan Li
The objective of the present study was to observe the effects of silibinin and the p38 mitogen-activated protein kinase (MAPK) signaling pathway inhibitor SB203580 on the migration and invasion capabilities of SGC7901 cells, and to explore the underlying associated mechanisms. Scratch, Transwell and Matrigel invasion assays were performed to study the effects of silibinin on cell migration and invasion. Western blot analysis was used to determine the expression levels of p38MAPK, phosphorylated (p-)p38MAPK, matrix metalloproteinase (MMP)-2 and MMP-9...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344154/study-of-mir-10b-regulatory-mechanism-for-epithelial-mesenchymal-transition-invasion-and-migration-in-nasopharyngeal-carcinoma-cells
#6
Weiyi Wang
The aim of the study was to investigate the miR-10b regulatory mechanism for epithelial-mesenchymal transition (EMT) and its effect on the proliferation and migration of nasopharyngeal carcinoma cells. RT-qPCR was used to detect the expression of miR-10b in CNE1 nasopharyngeal carcinoma cell line. The NP69 nasopharyngeal mucosal cell line was used to determine the expression of miR-10b after infection with lentivirus. The effect of miR-10b on the proliferation of NP69 was examined using cell counting kit-8...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344144/melanoma-differentiation-associated-gene-7-suppresses-human-gastric-cancer-cell-invasion-and-migration
#7
Li Xu, Jinyan Chen, Wei Lin, Jinkun Chen, Zhiwei Chen
Gastric cancer is one of the most common types of cancer in the world. Patients with gastric cancer often respond poorly to conventional chemotherapies, therefore more comprehensive therapy is required. Melanoma differentiation-associated gene-7 (MDA-7), also termed interleukin-24, is a potent tumor suppressor gene. Numerous studies have demonstrated that MDA-7 suppresses the growth and induces the apoptosis of cancer cells. In the present study, the MDA-7 gene was transfected into human gastric cancer AGS cells using adenovirus...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343955/lysyl-oxidase-activates-cancer-stromal-cells-and-promotes-gastric-cancer-progression-quantum-dot-based-identification-of-biomarkers-in-cancer-stromal-cells
#8
Chunwei Peng, Jiuyang Liu, Guifang Yang, Yan Li
Purpose: Semiconductor quantum dots (QDs) are a promising alternative to organic fluorescent dyes for multiplexed molecular imaging of cancer stroma, which have great advantages in holistically analyzing the complex interactions among cancer stromal components in situ. Patients and methods: A QD probe-based multiplexed spectral molecular imaging method was established for simultaneous imaging. Three tissue microarrays (TMAs) including 184 gastric cancer (GC) tissues were constructed for the study...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29343183/the-acute-angiogenic-signalling-response-to-low-load-resistance-exercise-with-blood-flow-restriction
#9
Richard A Ferguson, Julie E A Hunt, Mark P Lewis, Neil R W Martin, Darren J Player, Carolin Stangier, Conor W Taylor, Mark C Turner
This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT-PCR...
January 17, 2018: European Journal of Sport Science
https://www.readbyqxmd.com/read/29342922/can-egcg-alleviate-symptoms-of-down-syndrome-by-altering-proteolytic-activity
#10
REVIEW
Marzena Wyganowska-Świątkowska, Maja Matthews-Kozanecka, Teresa Matthews-Brzozowska, Ewa Skrzypczak-Jankun, Jerzy Jankun
Down syndrome (DS), also known as "trisomy 21", is a genetic disorder caused by the presence of all or part of a third copy of chromosome 21. Silencing these extra genes is beyond existing technology and seems to be impractical. A number of pharmacologic options have been proposed to change the quality of life and lifespan of individuals with DS. It was reported that treatment with epigallocatechin gallate (EGCG) improves cognitive performance in animal models and in humans, suggesting that EGCG may alleviate symptoms of DS...
January 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29342454/sodium-trimetaphosphate-as-a-novel-strategy-for-matrix-metalloproteinase-inhibition-and-dentin-remineralization
#11
Rafael Simões Gonçalves, Polliana Mendes Candia Scaffa, Marina Ciccone Giacomini, Cristina de Mattos Pimenta Vidal, Heitor Marques Honório, Linda Wang
The effect of sodium trimetaphosphate (STMP) as an antiproteolytic and remineralizing agent on demineralized dentin was evaluated in vitro. The inhibitory potential of STMP at 0.5, 1.5, 3.5, and 5% against recombinant matrix metalloproteinases (MMPs) MMPs-2 and -9 was assessed by zymography. To investigate its remineralization potential, 40 bovine root specimens were obtained and subjected to a demineralization protocol to produce caries-like dentin lesions. After that, dentin surfaces were divided into 3 areas: (1) mineralized (no treatment); (2) demineralized; and (3) demineralized/treated with STMP and submitted to a pH-cycling associated or not with STMP (1...
January 18, 2018: Caries Research
https://www.readbyqxmd.com/read/29339086/the-il-6-stat3-pathway-regulates-adhesion-molecules-and-cytoskeleton-of-endothelial-cells-in-thromboangiitis-obliterans
#12
Zheng Wei, Wenjing Jiang, Hengzhen Wang, Hali Li, Bo Tang, Bing Liu, Hongchi Jiang, Xueying Sun
Thromboangiitis obliterans (TAO) (also known as Buerger's disease) is an inflammatory vascular disease that predominantly affects small- and medium-sized blood vessels of extremities. Endothelial cells play critical roles in the initiation and progression of this disease, but the underlying mechanisms remain unclear. In the present study, we demonstrate that patients with TAO had significantly higher levels of interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in their plasmas, and the involved arterial tissues expressed higher levels of phosphorylated signal transducer and activator of transcription 3 (p-STAT3), ICAM-1 and VCAM-1...
January 12, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29338907/new-photodynamic-molecular-beacons-pmb-as-potential-cancer-targeted-agents-in-pdt
#13
Aurélie Stallivieri, Ludovic Colombeau, Jérôme Devy, Nicolas Etique, Carine Chaintreuil, Bauyrzhan Myrzakhmetov, Mathilde Achard, Francis Baros, Philippe Arnoux, Régis Vanderesse, Céline Frochot
Further improvements in Photodynamic therapy (PDT) necessitate that the dye targets more selectively tumour tissues or neovascularization than healthy cells. Different enzymes such as matrix metalloproteinases (MMPs) are overexpressed in tumour areas. Among these MMPs, gelatinases (MMP-2 and MMP-9) and its activator MMP-14 are known to play a key role in tumour angiogenesis and the growth of many cancers such as glioblastoma multiforme (GBM), an aggressive malignant tumour of the brain. These last years, the concept of photodynamic molecular beacons (PMB) became interesting for controlling the photosensitizer's ability to generate singlet oxygen (1O2) close to target biomolecules as MMPs...
December 24, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29337619/the-role-of-necroptosis-apoptosis-and-inflammation-in-fowl-cholera-associated-liver-injury-in-a-chicken-model
#14
Qiyu Tang, Weitian Li, Na Dai, Yiming Gao, Yu Han, Guofu Cheng, Changqin Gu
Fowl cholera resulting from infection with Pasteurella multocida causes huge economic losses in the poultry industry. Necrotic hepatitis is reported to be a significant lesion associated with fowl cholera in chickens. Clarifying the underlying molecular mechanism of hepatic injury caused by P. multocida infection is needed to develop new strategies to control fowl cholera. Pasteurella multocida Q (the standard reference strain) and P. multocida 1G1 (a clinical strain) were used to infect healthy laying hens...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29336497/effects-of-treatment-with-maraviroc-a-ccr5-inhibitor-on-a-human-hepatic-stellate-cell-line
#15
N Coppola, A Perna, A Lucariello, S Martini, M Macera, M A Carleo, G Guerra, V Esposito, A De Luca
After an acute liver damage, tissue regeneration repairs lesions with degradation of deposed fibrotic material, while mechanisms of tissue restoration are persistently activated following several repeated injuries, inducing deposition of extracellular matrix. (ECM). Factors responsible for ECM remodeling have been identified in a pathway involving a family of zinc-dependent enzyme matrix metalloproteinases (MMPs), together with tissue inhibitor of metalloproteinases (TIMPs). Recent experimental models suggested a role of CCR5 receptor in the genesis of liver fibrosis...
January 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29335864/ethanol-extract-of-lycopodium-serratum-thunb-attenuates-lipopolysaccharide-induced-c6-glioma-cells-migration-via-matrix-metalloproteinase-9-expression
#16
Ju-Yeon Park, Hyuck Kim, Dong-Woo Lim, Jai-Eun Kim, Won-Hwan Park, Sun-Dong Park
OBJECTIVE: To elucidate how ethanol extract of L. serratum (ELS) could exert anti-migratory effects on glioma with the suppression of nuclear factor kappa B (NF-κB) downstream pathway. METHODS: Cell viability of ELS on C6 glioma was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Nitric oxide (NO) assay and 2',7'-dichloroflfluorescin diacetate (DCFH-DA) assay were applied to measure NO production and reactive oxygen species (ROS) generation on lipopolysaccharide (LPS)-induced C6 glioma cells...
January 15, 2018: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29333768/-effects-of-geranylgeranyltransferase-%C3%A2-gene-silencing-by-rna-interference-on-the-migration-and-invasion-of-tongue-carcinoma
#17
Chen Zhenggang, Wang Shuren, Li Jinghua, Han Jinhong, Wang Qimin, Tong Lei, Liu Wenjun, Yang Fang, Guo Qingyuan, Guo Dawei, Wang Ying
OBJECTIVE: RNA interference was used to silence geranylgeranyltransferase Ⅰ(GGTase-Ⅰ) in vitro and to study the effect of GGTase-Ⅰ on the migration and invasion of tongue squamous cancer cells. METHODS: Three small interfering RNAs (siRNA) were designed according to the GGTase-Ⅰ sequence by Genebank and were transfected into tongue squamous cancer cells Cal-27 to knock down GGTase-Ⅰ expression. The tested cells were divided into three groups, as follows: the RNA-interfered groups (GGTase-Ⅰ siRNA1, GGTase-Ⅰ siRNA 2, GGTase-Ⅰ siRNA 3), a negative control group (disrupted by random sequence NC-siRNA), and a blank control group...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29329315/role-of-n-glycosylation-in-activation-of-prommp-9-a-molecular-dynamics-simulations-study
#18
Sonu Kumar, Piotr Cieplak
Human matrix metalloproteinase proMMP-9 is secreted as latent zymogen, which requires two-steps proteolytic activation. The secreted proMMP-9 is glycosylated at two positions: Asn38 and Asn120 located in the prodomain and catalytic domain, respectively. It has been demonstrated that glycosylation at Asn120 is required for secretion of the enzyme, while the role of Asn38 glycosylation is not well understood, but is usually linked to the activation process. One hypothesis stated that the Asn38 glycosylation might protect against proteolytic activation...
2018: PloS One
https://www.readbyqxmd.com/read/29328525/a-role-of-sialic-acid-containing-glycans-of-matrix-metalloproteinase-9-mmp-9-in-the-interaction-between-mmp-9-and-staphylococcal-superantigen-like-protein-5-ssl5
#19
Chisato Kurisaka, Teruaki Oku, Saotomo Itoh, Tsutomu Tsuji
The staphylococcal superantigen-like proteins (SSLs) exhibit no superantigenic activity but have recently been considered to act as immune suppressors. It was previously reported that SSL5 bound to P-selectin glycoprotein ligand-1 (PSGL-1) and matrix metalloproteinase (MMP)-9, leading to inhibition of leukocyte adhesion and invasion. These interactions were suggested to depend on sialic acid-containing glycans of MMP-9, but the roles of sialic acids in the interaction between SSL5 and MMP-9 are still controversial...
January 12, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29328479/the-human-umbilical-cord-stem-cells-improve-the-viability-of-oa-degenerated-chondrocytes
#20
Hao Wang, Xu Yan, Yuxin Jiang, Zheng Wang, Yufei Li, Qingdong Shao
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim of the present study was to explore the interaction between human umbilical cord stem cells and degenerated chondrocytes, and the therapeutic potential of human umbilical cord stem cells on degenerated chondrocytes. Human umbilical cord‑derived mesenchymal stem cells (hUC‑MSCs) were harvested from human umbilical cords, and flow cytometry was used to analyze the surface antigen markers, in addition, chondrogenic, osteogenic and adipogenic differentiation on the cells was investigated...
January 9, 2018: Molecular Medicine Reports
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