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Candida glabrata

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https://www.readbyqxmd.com/read/28330225/metagenomics-analysis-of-microbial-communities-associated-with-a-traditional-rice-wine-starter-culture-xaj-pitha-of-assam-india
#1
Sudipta Sankar Bora, Jyotshna Keot, Saurav Das, Kishore Sarma, Madhumita Barooah
This is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving Illumine-based whole genome shotgun (WGS) sequencing method. Metagenomic DNA was extracted from rice wine starter culture concocted by Ahom community of Assam and analyzed using a MiSeq(®) System. A total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. Data obtained from the use of several taxonomic profiling tools were compared with previously reported microbial diversity studies through the culture-dependent and culture-independent method...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329281/echinocandins-compared-to-fluconazole-for-candidemia-of-a-urinary-tract-source-a-propensity-score-analysis
#2
G Cuervo, C Garcia-Vidal, M Puig-Asensio, A Vena, Y Meije, M Fernández-Ruiz, E González-Barberá, M J Blanco-Vidal, A Manzur, C Cardozo, C Gudiol, J M Montejo, J Pemán, J Ayats, J M Aguado, P Muñoz, F Marco, B Almirante, J Carratalà
Background.: Whether echinocandins could be used to treat candidemia of a urinary tract source (CUTS) is unknown. We aimed to provide current epidemiological information of CUTS and to compare echinocandin to fluconazole treatment on CUTS outcomes. Methods.: A multicenter study of adult patients with candidemia was conducted in 9 hospitals. CUTS was defined as a candidemia with concomitant candiduria by the same organism associated with significant urological comorbidity...
February 27, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28326781/the-berkeleylactones-antibiotic-macrolides-from-fungal-coculture
#3
Andrea A Stierle, Donald B Stierle, Daniel Decato, Nigel D Priestley, Jeremy B Alverson, John Hoody, Kelly McGrath, Dorota Klepacki
A carefully timed coculture fermentation of Penicillium fuscum and P. camembertii/clavigerum yielded eight new 16-membered-ring macrolides, berkeleylactones A-H (1, 4, 6-9, 12, 13), as well as the known antibiotic macrolide A26771B (5), patulin, and citrinin. There was no evidence of the production of the berkeleylactones or A26771B (5) by either fungus when grown as axenic cultures. The structures were deduced from analyses of spectral data, and the absolute configurations of compounds 1 and 9 were determined by single-crystal X-ray crystallography...
March 22, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28326154/probiotic-lactobacillus-reuteri-has-antifungal-effects-on-oral-candida-species-in-vitro
#4
Mette Rose Jørgensen, Camilla Kragelund, Peter Østrup Jensen, Mette Kirstine Keller, Svante Twetman
Background: An alternative approach for managing Candida infections in the oral cavity by modulating the oral microbiota with probiotic bacteria has been proposed. Objective: The aim was to investigate the antifungal potential of the probiotic bacterium Lactobacillus reuteri (DSM 17938 and ATCC PTA 5289) against six oral Candida species (C. albicans, C. glabrata, C. krusei, C. tropicalis, C. dubliniensis, and C. parapsilosis). Design: The lactobacilli were tested for their ability to co-aggregate with and inhibit the growth of the yeasts assessed by spectrophotometry and the agar overlay inhibition assay...
2017: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/28321466/the-risk-and-clinical-outcome-of-candidemia-depending-on-underlying-malignancy
#5
Olivier Lortholary, Charlotte Renaudat, Karine Sitbon, Marie Desnos-Ollivier, Stéphane Bretagne, Françoise Dromer
PURPOSE: To assess the risk factors and outcomes associated with fungemia caused by the six most commonly occurring Candida species in patients with and without malignancies. METHODS: Analysis of the episodes of fungemia due to common Candida species in adults, based on an active hospital-based surveillance program (Paris area, France, 2002 to 2014). RESULTS: Of the 3417 patients (3666 isolates), 1164 (34.1%) had a solid tumor (45.7% digestive tract) and 586 (17...
March 20, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28320713/antimicrobial-percutaneous-nephrostomy-catheters-for-the-prevention-of-renal-infections-in-vitro-studies
#6
Nylev Vargas-Cruz, Ruth A Reitzel, Joel Rosenblatt, Mohamed Jamal, Ariel D Szvalb, Anne-Marie Chaftari, Ray Hachem, Issam Raad
Purpose - Percutaneous nephrostomy (PCN) catheters are the primary method for draining ureters obstructed by malignancy and preventing a decline of the renal function. However, PCN-related infections such as pyelonephritis and urosepsis remain a significant concern. Currently no antimicrobial PCNs are available for preventing infectious complications. Vascular catheters impregnated with Minocycline/Rifampin (M/R) and M/R with Chlorhexidine coating (M/R+CHD) have previously demonstrated antimicrobial activity...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28318900/epidemiological-and-mycological-characteristics-of-candidemia-in-iran-a-systematic-review-and-meta-analysis
#7
REVIEW
A Vaezi, H Fakhim, S Khodavaisy, A Alizadeh, M Nazeri, A Soleimani, T Boekhout, H Badali
To date, there has been no comprehensive review of the epidemiology, risk factors, species distribution, and outcomes of candidemia in Iran. This study aimed to perform a systematic review and meta-analysis of all reported candidemia cases in Iran until December 2015. The review process occurred in three steps, namely a literature search, data extraction and statistical analyses. After a comprehensive literature search, we identified 55 cases. The mean age of patients was 46.80±24.30 years (range 1-81 years)...
March 16, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28314677/vulvovaginal-candidiasis-etiology-symptomatology-and-risk-factors
#8
L Mtibaa, N Fakhfakh, A Kallel, S Belhadj, N Belhaj Salah, N Bada, K Kallel
OBJECTIVE: To determine epidemiological, clinical and mycological characteristics of vulvovaginal candidiasis (VVC) in Tunisian population and to evaluate predisposing factors. PATIENTS AND METHODS: In this retrospective study, 2160 vaginal swabs were performed over 2 years (January 2014-December 2015). It was carried out at the laboratory of Parasitology and Mycology, Rabta Hospital in Tunisia. After swab collecting, direct examination and culture on Sabouraud Chloramphenicol and Sabouraud Chloramphenicol Actidione media were implemented to research yeasts...
March 14, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28303122/antifungal-drug-susceptibility-of-candida-species-isolated-from-hiv-positive-patients-recruited-at-a-public-hospital-in-s%C3%A3-o-lu%C3%A3-s-maranh%C3%A3-o-brazil
#9
Ana L G Terças, Sirlei G Marques, Eduardo B Moffa, Márcia B Alves, Conceição M P S de Azevedo, Walter L Siqueira, Cristina A Monteiro
Oropharyngeal candidiasis is the most common fungal infection in hospitalized patients with acquired immune deficiency syndrome (AIDS). Its progression results in invasive infections, which are a significant cause of morbidity and mortality. This study aimed to quickly and accurately identify Candida spp. from oral mucosa of AIDS patients recruited at Presidente Vargas Hospital, in São Luís city, Brazil and to evaluate the sensitivity profile of these fungi to antifungals by using an automated system. Isolates were collected from oropharyngeal mucosa of 52 hospitalized AIDS patients, under anti-viral and antifungal therapies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28296967/the-combined-rapid-detection-and-species-level-identification-of-yeasts-in-simulated-blood-culture-using-a-colorimetric-sensor-array
#10
Nabin K Shrestha, Sung H Lim, Deborah A Wilson, Ana Victoria SalasVargas, Yair S Churi, Paul A Rhodes, Peter J Mazzone, Gary W Procop
BACKGROUND: A colorimetric sensor array (CSA) has been demonstrated to rapidly detect and identify bacteria growing in blood cultures by obtaining a species-specific "fingerprint" of the volatile organic compounds (VOCs) produced during growth. This capability has been demonstrated in prokaryotes, but has not been reported for eukaryotic cells growing in culture. The purpose of this study was to explore if a disposable CSA could differentially identify 7 species of pathogenic yeasts growing in blood culture...
2017: PloS One
https://www.readbyqxmd.com/read/28293217/mechanistic-insights-underlying-tolerance-to-acetic-acid-stress-in-vaginal-candida-glabrata-clinical-isolates
#11
Diana V Cunha, Sara B Salazar, Maria M Lopes, Nuno P Mira
During colonization of the vaginal tract Candida glabrata cells are challenged with the presence of acetic acid at a low pH, specially when dysbiosis occurs. To avoid exclusion from this niche C. glabrata cells are expected to evolve efficient adaptive responses to cope with this stress; however, these responses remain largely uncharacterized, especially in vaginal strains. In this work a cohort of 18 vaginal strains and 2 laboratory strains (CBS138 and KUE100) were phenotyped for their tolerance against inhibitory concentrations of acetic acid at pH 4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28284180/candida-krusei-form-mycelia-along-agar-surfaces-towards-each-other-and-other-candida-species
#12
Jacob Fleischmann, Corey D Broeckling, Sarah Lyons
BACKGROUND: Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiments to study this interaction. RESULTS: When C.krusei yeast cells are plated in parallel streaks, they form mycelia along agar surfaces toward other yeasts...
March 11, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28282487/establishment-of-multiplex-solid-phase-strip-pcr-test-for-detection-of-24-ocular-infectious-disease-pathogens
#13
Satoko Nakano, Sunao Sugita, Yasuhiro Tomaru, Ayumi Hono, Takako Nakamuro, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Masayo Takahashi, Norio Shimizu
Purpose: To establish and evaluate a new multiplex solid-phase strip polymerase chain reaction (strip PCR) for concurrent detection of common ocular infectious disease pathogens. Methods: A new multiplex strip PCR was established to detect 24 common ocular infectious disease pathogens: herpes simplex virus (HSV) 1, HSV2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus (HHV) 6, HHV7, HHV8, human T-cell lymphotropic virus (HTLV)-1, adenovirus, Mycobacterium tuberculosis, Treponema pallidum, Propionibacterium acnes (P...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28280017/draft-genome-sequences-of-candida-glabrata-isolates-1a-1b-2a-2b-3a-and-3b
#14
Othilde Elise Håvelsrud, Peter Gaustad
Here, we report the draft genome sequences of six Candida glabrata isolates. The isolates were taken from blood samples from patients after recurrent C. glabrata infection. Two isolates were taken from each of three patients a minimum 3 months apart.
March 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28275534/disseminated-phaeohyphomycosis-in-a-dog
#15
Lana S Rothenburg, Timothy A Snider, Allison Wilson, Anthony W Confer, Akhilesh Ramachandran, Rinosh Mani, Theresa Rizzi, Laura Nafe
Phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. Here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to Bipolaris spicifera infection. Regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. Candida glabrata and Fusarium oxysporum were also cultured from multiple sites post-mortem. The dog was treated with fluconazole, itraconazole, terbinafine and liposomal amphotericin B, but was euthanized due to its poor prognosis after 12 days of therapy...
March 2017: Medical Mycology Case Reports
https://www.readbyqxmd.com/read/28271321/clinical-characteristics-and-predictors-of-mortality-in-cirrhotic-patients-with-candidemia-and-intra-abdominal-candidiasis-a-multicenter-study
#16
Matteo Bassetti, Maddalena Peghin, Alessia Carnelutti, Elda Righi, Maria Merelli, Filippo Ansaldi, Cecilia Trucchi, Cristiano Alicino, Assunta Sartor, Pierluigi Toniutto, Joost Wauters, Wim Laleman, Carlo Tascini, Francesco Menichetti, Roberto Luzzati, Pierluigi Brugnaro, Alessio Mesini, Stefania Raviolo, Francesco G De Rosa, Leonel Lagunes, Jordi Rello, George Dimopoulos, Arnaldo L Colombo, Marcio Nucci, Antonio Vena, Emilio Bouza, Patricia Muñoz, Mario Tumbarello, Raffaella Losito, Ignacio Martin-Loeches, Claudio Viscoli
PURPOSE: The aim of the study was to describe the characteristics of cirrhotic patients with candidemia and intra-abdominal candidiasis (IAC) and to evaluate the risk factors associated with 30-day mortality. METHODS: A multicenter multinational retrospective study including all consecutive episodes of candidemia and IAC in adult patients with liver cirrhosis in 14 European hospitals during the period 2011-2013 was performed. RESULTS: A total of 241 episodes (169 candidemia, 72 IAC) were included...
March 7, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28264843/a-risk-score-for-fluconazole-failure-among-patients-with-candidemia
#17
Luis Ostrosky-Zeichner, Rachel Harrington, Nkechi Azie, Hongbo Yang, Nanxin Li, Jing Zhao, Valerie Koo, Eric Q Wu
Background: This study aimed to develop a prediction model to identify patients with candidemia at high risk of failing fluconazole.Methods: Adult patients in the US with candidemia who received fluconazole during hospitalization were selected from the Cerner Health Facts® database (04/2004-03/2013). Fluconazole failure was defined as switching/adding another antifungal, positive Candida culture ≥10 days after fluconazole initiation, or death during hospitalization. Patients were randomized into modeling and validation samples...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28261161/antibacterial-activity-and-synergistic-antibacterial-potential-of-biosynthesized-silver-nanoparticles-against-foodborne-pathogenic-bacteria-along-with-its-anticandidal-and-antioxidant-effects
#18
Jayanta Kumar Patra, Kwang-Hyun Baek
Silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. These nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. Green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. However, few studies have investigated the use of industrial waste materials in nanoparticle synthesis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28260588/utilization-of-size-polymorphism-in-its1-and-its2-regions-for-identification-of-pathogenic-yeast-species
#19
Hossein Khodadadi, Ladan Karimi, Nilufar Jalalizand, Hassan Adin, Hossein Mirhendi
PURPOSE: Despite the existence of a variety of available yeast-identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. The internal transcribed spacer (ITS) regions of fungal rRNA genes exhibit variable sizes depending on the yeast species. In the present study, fragment size polymorphism (FSP) analysis of the ITS1 and ITS2 regions for identification of the clinically most important yeast species was assessed. METHODOLOGY: The ITS1 and ITS2 regions of 190 strains, including isolates of 31 standard strains and 159 clinical isolates, were separately PCR amplified with two primer sets: ITS1-ITS2 and ITS3-ITS4...
March 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28258218/structural-analyses-of-candida-albicans-sterol-14%C3%AE-demethylase-complexed-with-azole-drugs-address-the-molecular-basis-of-azole-mediated-inhibition-of-fungal-sterol-biosynthesis
#20
Tatiana Y Hargrove, Laura Friggeri, Zdzislaw Wawrzak, Aidong Qi, William J Hoekstra, Robert J Schotzinger, John D York, F Peter Guengerich, Galina I Lepesheva
With some advances in modern medicine (such as cancer chemotherapy, broad exposure to antibiotics, and immunosuppression) the incidence of opportunistic fungal pathogens such as Candida albicans has increased. Cases of drug resistance among these pathogens have become more frequent, requiring the development of new drugs and a better understanding of the targeted enzymes. Sterol 14α-demethylase (CYP51) is a cytochrome P450 enzyme required for biosynthesis of sterols in eukaryotic cells and the major target of clinical drugs for managing fungal pathogens, but some of the CYP51 key features important for rational drug design have remained obscure...
March 3, 2017: Journal of Biological Chemistry
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