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https://www.readbyqxmd.com/read/28651380/utilization-of-acid-pre-treated-coconut-dregs-as-a-substrate-for-production-of-detergent-compatible-lipase-by-bacillus-stratosphericus
#1
Nur Bainun Mohd Zin, Busyra Mohamad Yusof, Siti Nurbaya Oslan, Helmi Wasoh, Joo Shun Tan, Arbakariya B Ariff, Murni Halim
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28651365/cathepsin-k-inhibitors-for-osteoporosis-biology-potential-clinical-utility-and-lessons-learned
#2
Matthew T Drake, Bart L Clarke, Merry Jo Oursler, Sundeep Khosla
Cathepsin K is a cysteine protease member of the cathepsin lysosomal protease family. While cathepsin K is highly expressed in osteoclasts, lower levels of cathepsin K are also found in a variety of other tissues. Secretion of cathepsin K from the osteoclast into the sealed osteoclast-bone cell interface results in efficient degradation of type I collagen. The absence of cathepsin K activity in humans results in pycnodysostosis, characterized by increased bone mineral density and fractures. Pharmacological cathepsin K inhibition leads to continuous increases in BMD for up to five years of treatment and improves bone strength at the spine and hip...
June 23, 2017: Endocrine Reviews
https://www.readbyqxmd.com/read/28651347/minimally-invasive-centrimag-ventricular-assist-device-support-integrated-with-extracorporeal-membrane-oxygenation-in-cardiogenic-shock-patients-a-comparison-with-conventional-centrimag-biventricular-support-configuration
#3
Koji Takeda, Arthur R Garan, Masahiko Ando, Jiho Han, Veli K Topkara, Paul Kurlansky, Melana Yuzefpolskaya, Maryjane A Farr, Paolo C Colombo, Yoshifumi Naka, Hiroo Takayama
OBJECTIVES: We recently developed a novel minimally invasive surgical approach that combines extracorporeal membrane oxygenation and CentriMag ventricular assist device (Ec-VAD) for the treatment of cardiogenic shock as a short-term circulatory support. We compared the outcomes of this new approach to conventional CentriMag biventricular assist device (BiVAD) support through a median sternotomy. METHODS: Between July 2015 and August 2016, 22 patients were implanted with CentriMag Ec-VAD and 90 patients were implanted with conventional CentriMag BiVAD...
June 22, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28651324/preparation-of-activated-carbon-from-molasses-to-ethanol-process-waste-vinasse-and-its-performance-as-adsorbent-material
#4
Omer Kazak, Yasin Ramazan Eker, Haluk Bingol, Ali Tor
Herein, the preparation of activated carbon from waste vinasse by using hydrothermal H2O2 oxidation combined with a two-step pyrolysis process has been described for the first time as a new utilization approach for the waste vinasse. The characterization studies were performed by TGA, FT-IR, Raman spectroscopy, SEM-EDX and gas adsorption measurements. The approach generated a microporous activated carbon with high specific surface area (989m(2)/g) from waste vinasse. After simple experiments on various dye solutions, adsorption performance of the activated carbon was specifically studied on the methylene blue solution as functions of solution pH, contact time, adsorbent amount and reusability...
June 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651319/utilization-of-rice-husks-functionalized-with-xanthates-as-cost-effective-biosorbents-for-optimal-cd-ii-removal-from-aqueous-solution-via-response-surface-methodology
#5
Jianhua Qu, Xianlin Meng, Hong You, Xiuqing Ye, Zhaolin Du
Pristine rice husk (RH), a kind of agricultural waste based biosorbent, was chemically modified by introducing sulfur-bearing groups with the CS2 treatment under caustic medium. Using response surface methodology (RSM), the interactive effects of three preparation variables on the cadmium uptake and material yield of xanthate-modified rick husk (RH-X) were investigated, and two quadratic regression models were developed to correlate the preparation parameters to the two responses. Through process optimization, the optimal RH-X was finally obtained under the optimum synthesis conditions (i...
June 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651291/development-of-a-reference-standard-library-of-chloroplast-genome-sequences-genometrakrcp
#6
Ning Zhang, Padmini Ramachandran, Jun Wen, James A Duke, Helen Metzman, William McLaughlin, Andrea R Ottesen, Ruth E Timme, Sara M Handy
Precise, species-level identification of plants in foods and dietary supplements is difficult. While the use of DNA barcoding regions (short regions of DNA with diagnostic utility) has been effective for many inquiries, it is not always a robust approach for closely related species, especially in highly processed products. The use of fully sequenced chloroplast genomes, as an alternative to short diagnostic barcoding regions, has demonstrated utility for closely related species. The U. S. Food and Drug Administration (FDA) has also developed species-specific DNA-based assays targeting plant species of interest by utilizing chloroplast genome sequences...
June 26, 2017: Planta Medica
https://www.readbyqxmd.com/read/28651261/evaluation-of-the-value-of-contrast-enhanced-ultrasound-ceus-within-radiology-departments-in-germany
#7
Tilmann Graeter, Wolfgang Kratzer, Thomas Seufferlein, Suemeyra Tasdemir, Aylin Senguel, Julian Schmidberger, Hartmut Graeter, Christian Stroszczynski, Meinrad Beer
Purpose Contrast-enhanced ultrasound (CEUS) has been established for many years and has become an increasingly important cornerstone of the diagnostic imaging of various organ systems. However, this modality is rarely performed by radiologists and is primarily employed by other medical disciplines. The goal of this questionnaire study is to present the current value of CEUS in radiology and to encourage reevaluation of the method within this medical discipline. Materials and Methods 560 directors of radiology departments throughout Germany were contacted and given a 3-page questionnaire...
June 26, 2017: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/28651229/coverage-of-complex-defects-of-the-shoulder-girdle-and-posterior-neck-triangle-following-tumor-resection
#8
Noha M Al-Qattan, Mohammad M Al-Qattan, Rajeev Pant
INTRODUCTION: Numerous pedicle and free flaps have been used to cover complex defects of the shoulder girdle and posterior neck triangle following tumor resection. We describe our choice of flap selection in these patients with case examples. PRESENTATION OF CASES: Three cases examples demonstrate our choice of flap selection. In the first case, an anterior shoulder girdle defect is covered by an anteriorly transposed latissimus dorsi muscle flap. The second case demonstrates the coverage of a posterior shoulder girdle defect by a posteriorly transposed latissimus dorsi muscle flap...
June 13, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28651183/investigating-the-effect-of-different-transducer-stiffness-values-on-the-contactin-complex-detachment-by-steered-molecular-dynamics
#9
Parnian Kianfar, Nabiollah Abolfathi, Navid Zarif Karimi
This study investigated the adhesion behavior of Contactin4 (CNTN4), a member of Immunoglobulin Super Family (Ig-SF) of cell adhesion molecules. Contactin4 plays a crucial role in the formation, maintenance, and plasticity of neuronal networks. Contactin in its complex configuration with protein tyrosine phosphatase gamma (PTPRG) was selected for simulation. By utilizing Steered Molecular Dynamics (SMD), the uniaxial force was applied to induce unbinding of the complex, and the force-induced detachment of complex components was probed...
June 2, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28651178/the-influence-of-impact-direction-and-axial-loading-on-the-bone-fracture-pattern
#10
Haim Cohen, Chen Kugel, Hila May, Bahaa Medlej, Dan Stein, Viviane Slon, Tamar Brosh, Israel Hershkovitz
The effect of the direction of the impact and the presence of axial loading on fracture patterns have not yet been established in experimental 3-point bending studies. PURPOSE: To reveal the association between the direction of the force and the fracture pattern, with and without axial loading. MATERIAL AND METHODS: A Dynatup Model POE 2000 (Instron Co.) low energy pendulum impact machine was utilized to apply impact loading on fresh pig femoral bones (n=50). The bone clamp shaft was adjusted to position the bone for three-point bending with and without additional bone compression...
May 22, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28651171/the-relevance-of-inter-and-intrastrain-differences-in-mice-and-rats-and-their-implications-for-models-of-seizures-and-epilepsy
#11
REVIEW
Wolfgang Löscher, Russell J Ferland, Thomas N Ferraro
It is becoming increasingly clear that the genetic background of mice and rats, even in inbred strains, can have a profound influence on measures of seizure susceptibility and epilepsy. These differences can be capitalized upon through genetic mapping studies to reveal genes important for seizures and epilepsy. However, strain background and particularly mixed genetic backgrounds of transgenic animals need careful consideration in both the selection of strains and in the interpretation of results and conclusions...
June 23, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28651153/the-role-of-marijuana-use-disorder-in-predicting-emergency-department-and-inpatient-encounters-a-retrospective-cohort-study
#12
Cynthia I Campbell, Amber L Bahorik, Andrea H Kline-Simon, Derek D Satre
BACKGROUND: Marijuana use disorder (MUD) is the most common illegal drug use disorder and its prevalence is increasing. It is associated with psychiatric and medical problems, but little is known about its impact on emergency department (ED) and inpatient utilization rates. DESIGN: In a retrospective cohort design, we used electronic health record (EHR) data to identify patients with MUD (n=2752) and demographically matched patients without MUD (n=2752) in 2010...
June 10, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28651135/a-model-biorefinery-for-avocado-persea-americana-mill-processing
#13
Javier A Dávila, Moshe Rosenberg, Eulogio Castro, Carlos A Cardona
This research investigated and evaluated a biorefinery for processing avocado Hass variety into microencapsulated phenolic compounds extract, ethanol, oil and xylitol. Avocado was first characterized for its potential valuable compounds; then, the techno-economic and environmental aspects of the biorefinery were developed and finally the total production costs and potential environmental impact of the proposed biorefinery were investigated. Four scenarios of the biorefinery were evaluated with different extent of mass and energy integration as well as the incorporation of a cogeneration system...
June 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651132/enhanced-acetone-butanol-ethanol-production-from-lignocellulosic-hydrolysates-by-using-starchy-slurry-as-supplement
#14
Ming Yang, Suvi Kuittinen, Jouko Vepsäläinen, Junhua Zhang, Ari Pappinen
This study aims to improve acetone-butanol-ethanol production from the hydrolysates of lignocellulosic material by supplementing starchy slurry as nutrients. In the fermentations of glucose, xylose and the hydrolysates of Salix schwerinii, the normal supplements such as buffer, minerals, and vitamins solutions were replaced with the barley starchy slurry. The ABE production was increased from 0.86 to 14.7g/L by supplementation of starchy slurry in the fermentation of xylose and the utilization of xylose increased from 29% to 81%...
June 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651048/nutritional-and-metabolic-characteristics-of-brassica-carinata-coproducts-from-biofuel-processing-in-dairy-cows
#15
Yajing Ban, Nazir Ahmad Khan, Peiqiang Yu
The increased utilization of Brassica carinata in the biofuel industry in Canada has resulted in the large scale production of coproducts that can be potentially exploited as alternative protein ingredient in dairy ration. The objectives of this study were to investigate the nutritive value of carinata presscake and meal for dairy cows in terms of (1) nutrient and anti-nutrient composition; (2) rumen degradation kinetics of organic matter (OM), crude protein (CP) and neutral detergent fiber; (3) hourly effective degradation ratio and potential N to energy synchronization; (4) intestinal digestion of rumen undegraded protein (RUP); and (5) total metabolizable protein (MP) supply to the small intestine...
June 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28651043/visualizing-the-contributions-of-virtual-states-to-two-photon-absorption-cross-sections-by-natural-transition-orbitals-of-response-transition-density-matrices
#16
Kaushik D Nanda, Anna I Krylov
Observables such as two-photon absorption cross-sections cannot be computed from the wave functions of the initial and final states alone because of their nonlinear nature. Rather, they depend on the entire manifold of the excited states, which follows from the familiar sum-over-states expressions of second- and higher-order properties. Consequently, the interpretation of the computed nonlinear optical properties in terms of molecular orbitals is not straightforward and usually relies on approximate few-states models...
June 26, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28651026/binding-of-human-plasminogen-and-high-molecular-mass-kininogen-by-cell-surface-exposed-proteins-of-candida-parapsilosis
#17
Justyna Karkowska-Kuleta, Dorota Zajac, Grazyna Bras, Oliwia Bochenska, Maria Rapala-Kozik, Andrzej Kozik
Pathogenic microbes can recruit to their cell surface human proteins that are components of important proteolytic cascades involved in coagulation, fibrinolysis and innate immune response. Once located at the bacterial or fungal surface, such deployed proteins might be utilized by pathogens to facilitate invasion and dissemination within the host organism by interfering with functionality of these systems or by exploiting specific activity of the bound enzymes. Aim of the study presented here was to characterize this phenomenon in Candida parapsilosis (Ashford) Langeron et Talice - an important causative agent of systemic fungal infections (candidiases and candidemias) in humans...
June 24, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28651022/can-natural-variation-in-grain-p-concentrations-be-exploited-in-rice-breeding-to-lower-fertilizer-requirements
#18
Fanmiao Wang, James Douglas Morrison King, Terry Rose, Tobias Kretzschmar, Matthias Wissuwa
Agricultural usage of phosphorus (P) is largely driven by the amount of P removed from fields in harvested plant matter as offtake needs to be balanced by P fertilizer application. Reducing P concentration in grains is a way to decrease P offtake and reduce P fertilizer requirements or soil P mining where insufficient P is applied. Our objective was to assesses the genotypic variation for grain P concentration present within the rice gene pool and resolve to what extent it is affected by environment (P supply) or associated with genetic factors...
2017: PloS One
https://www.readbyqxmd.com/read/28651016/enhanced-light-microscopy-visualization-of-virus-particles-from-zika-virus-to-filamentous-ebolaviruses
#19
George G Daaboul, David S Freedman, Steven M Scherr, Erik Carter, Alexandru Rosca, David Bernstein, Chad E Mire, Krystle N Agans, Thomas Hoenen, Thomas W Geisbert, M Selim Ünlü, John H Connor
Light microscopy is a powerful tool in the detection and analysis of parasites, fungi, and prokaryotes, but has been challenging to use for the detection of individual virus particles. Unlabeled virus particles are too small to be visualized using standard visible light microscopy. Characterization of virus particles is typically performed using higher resolution approaches such as electron microscopy or atomic force microscopy. These approaches require purification of virions away from their normal millieu, requiring significant levels of expertise, and can only enumerate small numbers of particles per field of view...
2017: PloS One
https://www.readbyqxmd.com/read/28651012/the-economic-burden-of-human-papillomavirus-related-precancers-and-cancers-in-sweden
#20
Ellinor Östensson, Maria Silfverschiöld, Lennart Greiff, Christine Asciutto, Johan Wennerberg, Marie-Louise Lydryp, Ulf Håkansson, Pär Sparén, Christer Borgfeldt
BACKGROUND: High-risk (HR) human papillomavirus (HPV) infection is an established cause of malignant disease. We used a societal perspective to estimate the cost of HR HPV-related cervical, vulvar, vaginal, anal, and penile precancer and cancer, and oropharyngeal cancer in Sweden in 2006, 1 year before HPV vaccination became available in the country. MATERIALS AND METHODS: This prevalence-based cost-of-illness study used diagnosis-specific data from national registries to determine the number of HR HPV-related precancers and cancers...
2017: PloS One
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