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Beijing genotype and mycobacterium tuberculosis

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https://www.readbyqxmd.com/read/28919549/the-predominance-of-ethiopian-specific-mycobacterium-tuberculosis-families-and-minimal-contribution-of-mycobacterium-bovis-in-tuberculous-lymphadenitis-patients-in-southwest-ethiopia
#1
Mulualem Tadesse, Gemeda Abebe, Alemayehu Bekele, Mesele Bezabih, Pim de Rijk, Conor J Meehan, Bouke C de Jong, Leen Rigouts
BACKGROUND: Ethiopia has an extremely high rate of extrapulmonary tuberculosis, dominated by tuberculous lymphadenitis (TBLN). However, little is known about Mycobacterium tuberculosis complex (MTBc) lineages responsible for TBLN in Southwest Ethiopia. METHODS: A total of 304 MTBc isolates from TBLN patients in Southwest Ethiopia were genotyped primarily by spoligotyping. Isolates of selected spoligotypes were further analyzed by 15-loci mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) (n=167) and qPCR-based single nucleotide polymorphism (n=38)...
September 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28910939/-genotyping-and-drug-susceptibility-of-mycobacterium-tuberculosis-isolated-in-changping-district-in-beijing-2011-2015
#2
Z G Zhang, J F Wu, L Wang, L Q Wang, L Han, S B Ma, T J Gao
Objective: To understand the genotype distribution of Mycobacterium tuberculosis and the drug susceptibility of M. tuberculosis with different genotypes in Changping district of Beijing and evaluate the application of genotyping of M. tuberculosis in local tuberculosis (TB) prevention and control. Methods: A total of 1 099 M. tuberculosis strains isolated in Changping from 2011 to 2015 were used. Spoligotyping and 12-locus VNTR recommended by Gao were used for the genotyping of these isolates. In addition, the susceptibility of the M...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28869227/a-study-on-beijing-genotype-in-the-clinical-isolates-of-pulmonary-drug-resistant-tuberculosis
#3
Ankit Kumar, Ajay Kumar Verma, Surya Kant, Ved Prakash, Anand Srivastava, Kanchan Srivastava, Amita Jain, Kishore K Srivastava
BACKGROUND: Mycobacterium tuberculosis (MTB) Beijing strains are spread worldwide and are responsible for major outbreaks of tuberculosis (TB), sometimes spreading multidrug resistance (MDR). AIM: The aim of this study was to explore clinical features associated with the infection with Beijing strains among MDR patients of pulmonary TB in Lucknow and surrounding areas. MATERIALS AND METHODS: It was a hospital-based epidemiological study. Our study population was selected from all the newly diagnosed patients attending outpatient department and admitted patients of Department of Respiratory Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India...
September 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28835627/evolutionary-pathway-analysis-and-unified-classification-of-east-asian-lineage-of-mycobacterium-tuberculosis
#4
Egor Shitikov, Sergey Kolchenko, Igor Mokrousov, Julia Bespyatykh, Dmitry Ischenko, Elena Ilina, Vadim Govorun
Due to its rapid spread and association with the numerous outbreaks, the global spread of East Asian lineage of Mycobacterium tuberculosis strains presents a global concern. Although there were many attempts to describe its population structure, no consensus has been reached yet. To define unbiased classification that will facilitate future studies of this lineage, we analyzed the performance and congruence of eight different genotyping schemes based on phylogenetic analysis of 1,398 strains from 32 countries using whole-genome sequencing (WGS) data...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28789982/mycobacterium-tuberculosis-genotypes-and-predominant-clones-among-the-multidrug-resistant-isolates-in-spain-1998-2005
#5
Sofía Samper, Patricia Gavín, María Isabel Millán-Lou, María José Iglesias, María Soledad Jiménez, David Couvin, Nalin Rastogi
Although the incidence of tuberculosis (TB) is gradually decreasing in Spain, there is an increase in the proportion of foreign-born cases. This changing scenario is slowly shifting the local TB epidemiology from endemic to imported cases with an increased risk for multidrug-resistant (MDR) and extensively drug resistant (XDR) strains of Mycobacterium tuberculosis complex. MDR/XDR strains from Spain (n=366 MTBC isolates, 1 strain per patient) isolated between 1998 and 2005 were retained for this retrospective analysis...
August 5, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28745309/a-quantitative-and-efficient-approach-to-select-miru-vntr-loci-based-on-accumulation-of-the-percentage-differences-of-strains-for-discriminating-divergent-mycobacterium-tuberculosis-sublineages
#6
Xin-Ling Pan, Chun-Lei Zhang, Chie Nakajima, Jin Fu, Chang-Xia Shao, Li-Na Zhao, Jia-Yi Cui, Na Jiao, Chang-Long Fan, Yasuhiko Suzuki, Toshio Hattori, Di Li, Hong Ling
Although several optimal mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) loci have been suggested for genotyping homogenous Mycobacterium tuberculosis, including the Beijing genotype, a more efficient and convenient selection strategy for identifying optimal VNTR loci is needed. Here 281 M. tuberculosis isolates were analyzed. Beijing genotype and non-Beijing genotypes were identified, as well as Beijing sublineages, according to single nucleotide polymorphisms. A total of 22 MIRU-VNTR loci were used for genotyping...
July 26, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28734764/comparative-genomic-analysis-of-mycobacterium-tuberculosis-beijing-like-strains-revealed-specific-genetic-variations-associated-with-virulence-and-drug-resistance
#7
Juan Germán Rodríguez-Castillo, Camilo Pino, Luis Fernando Niño, Juan Carlos Rozo, Claudia Llerena-Polo, Carlos A Parra-López, Andreas Tauch, Martha Isabel Murcia-Aranguren
Isolates of the Mycobacterium tuberculosis lineage 2/East-Asian are considered one of the most successful strains due to their increased pathogenicity, hyper-virulence associated with drug resistance, and high transmission. Recent studies in Colombia have shown that the Beijing-like genotype is associated with multidrug-resistance and high prevalence in the southwest of the country, but the genetic basis of its success in dissemination is unknown. In contribution to this matter, we obtained the whole sequences of six genomes of clinical isolates assigned to the Beijing-like genotype...
July 20, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28710410/combining-molecular-typing-and-spatial-pattern-analysis-to-identify-areas-of-high-tuberculosis-transmission-in-a-moderate-incidence-county-in-taiwan
#8
Yih-Yuan Chen, Jia-Ru Chang, Chih-Da Wu, Yen-Po Yeh, Shiu-Ju Yang, Chih-Hao Hsu, Ming-Ching Lin, Ching-Fang Tsai, Ming-Shian Lin, Ih-Jen Su, Horng-Yunn Dou
In total, 303 randomly selected clinical Mycobacterium tuberculosis (MTB) isolates from 303 patients (collected January to December 2012) in central Taiwan were examined. The major lineages found were Beijing (N = 114, 37.62%), Haarlem (N = 76, 25.08%) and East African-Indian (EAI) (N = 42, 13.86%). Notably, younger persons (≤30 years old) were 6.58 times more likely to be infected with a Beijing genotype compared to older persons (>70 years) (p < 0.05). Combining molecular typing methods and geographical information system (GIS) analysis, we uncovered a twofold higher incidence of Beijing strains in a hotspot area (33%) compared to non-hotspot areas (17%)...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28684565/complete-genome-sequences-of-three-representative-mycobacterium-tuberculosis-beijing-family-strains-belonging-to-distinct-genotype-clusters-in-hanoi-vietnam-during-2007-to-2009
#9
Takayuki Wada, Minako Hijikata, Shinji Maeda, Nguyen Thi Le Hang, Pham Huu Thuong, Nguyen Phuong Hoang, Nguyen Van Hung, Naoto Keicho
We present here three complete genome sequences of Mycobacterium tuberculosis Beijing family strains isolated in Hanoi, Vietnam. These three strains were selected from major genotypic clusters (15-MIRU-VNTR) identified in a previous population-based study. We emphasize their importance and potential as reference strains in this Asian region.
July 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28659320/development-of-a-one-step-multiplex-pcr-assay-for-differential-detection-of-major-mycobacterium-species
#10
Hansong Chae, Seung Jung Han, Su-Young Kim, Chang-Seok Ki, Hee Jae Huh, Dongeun Yong, Won-Jung Koh, Sung Jae Shin
The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of this assay were to (i) discriminate between the Mycobacterium tuberculosis complex (MTBC) and NTM using rv0577 or RD750, (ii) differentiate M...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28610791/the-role-of-the-time-kill-kinetics-assay-as-part-of-a-preclinical-modeling-framework-for-assessing-the-activity-of-anti-tuberculosis-drugs
#11
Hannelore I Bax, Irma A J M Bakker-Woudenberg, Corné P de Vogel, Aart van der Meijden, Annelies Verbon, Jurriaan E M de Steenwinkel
Novel treatment strategies for tuberculosis are urgently needed. Many different preclinical models assessing anti-tuberculosis drug activity are available, but it is yet unclear which combination of models is most predictive of clinical treatment efficacy. The aim of this study was to determine the role of our in vitro time kill-kinetics assay as an asset to a predictive preclinical modeling framework assessing anti-tuberculosis drug activity. The concentration- and time-dependent mycobacterial killing capacities of six anti-tuberculosis drugs were determined during exposure as single drugs or in dual, triple and quadruple combinations towards a Mycobacterium tuberculosis Beijing genotype strain and drug resistance was assessed...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28610789/comparative-study-of-genotypes-of-mycobacterium-tuberculosis-from-a-northern-indian-setting-with-strains-reported-from-other-parts-of-india-and-neighboring-countries
#12
Pragya Sharma, Kiran Katoch, Shilpi Chandra, Devendra Singh Chauhan, Vishnu Dutt Sharma, David Couvin, Nalin Rastogi, Vishwa Mohan Katoch
This study was carried out to characterize Mycobacterium tuberculosis population in Ghatampur, Kanpur, North India, by spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTRs) typing. A total of 335 isolates were genotyped by spoligotyping and Central Asian (CAS) sub-lineage was the most prevalent, comprising 59.1% of all isolates. Other lineages were: East-African Indian (EAI) (19.10%), T (5.07%), Beijing (3.28%), Manu (2.98%), X (2.68%), S (0.89%), H3 (0...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28596961/first-insight-into-the-molecular-epidemiology-of-mycobacterium-tuberculosis-isolates-from-the-minority-enclaves-of-southwestern-china
#13
Lianyong Chen, Yu Pang, Li Ma, Huijuan Yang, Haohao Ru, Xing Yang, Shuangqun Yan, Mao Jia, Lin Xu
Yunnan is a province located in southwestern China. The aim of this study was to investigate the genetic diversity of Mycobacterium tuberculosis (MTB) strains circulating in Yunnan Province. We used spoligotyping and a 12-locus high-resolution VNTR set to identify a total of 271 MTB isolates collected from six prefectures' Yunnan. All the 271 patients were classified as 11 different ethnic groups, including 133 Han patients (49.1%) and 138 minority patients (50.9%). Spoligotyping analyses revealed that the largest two spoligotypes were SIT1 (Beijing family, n = 136) and SIT53 (T family, n = 35)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28588286/mycobacterium-tuberculosis-beijing-genotype-family-strain-isolated-from-naturally-infected-plateau-zokor-myospalax-baileyi-in-china
#14
Lin Sun, Xiang Chen, Wenhai Zhang, Gencheng Huang, Yuanyuan Zhang, Zhengzhong Xu, Baofa Yin, Wanhong Wei, Xinan Jiao, Kanglin Wan
No abstract text is available yet for this article.
June 7, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28550826/-multidrug-resistant-tuberculosis-by-strains-of-beijing-family-in-patients-from-lisbon-portugal-preliminary-report
#15
Fernando Maltez, Teresa Martins, Diana Póvoas, João Cabo, Helena Peres, Francisco Antunes, João Perdigão, Isabel Portugal
INTRODUCTION: Beijing family strains of Mycobacterium tuberculosis are associated with multidrug-resistance. Although strains of the Lisboa family are the most common among multidrug-resistant and extensively drug-resistant patients in the region, several studies have reported the presence of the Beijing family. However, the features of patients from whom they were isolated, are not yet known. MATERIAL AND METHODS: Retrospective study involving 104 multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis, from the same number of patients, isolated and genotyped between 1993 and 2015 in Lisbon...
March 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28545488/genetic-diversity-of-mycobacterium-tuberculosis-isolates-from-tochigi-prefecture-a-local-region-of-japan
#16
Fuminori Mizukoshi, Tohru Miyoshi-Akiyama, Hiroki Iwai, Takako Suzuki, Reiko Kiritani, Teruo Kirikae, Keiji Funatogawa
BACKGROUND: Foreign-born patients with tuberculosis (TB) may introduce globally disseminated isolates of Mycobacterium tuberculosis into large cities in Japan. The risk of dissemination of these isolates into local regions, however, has not been determined. This study analyzed the molecular epidemiology of M. tuberculosis isolates obtained from TB patients living in a local region of Japan. METHODS: Whole genome sequences of 169 M. tuberculosis isolates, obtained from 148 Japanese-born and 21 foreign-born patients living in Tochigi, Japan, were analyzed using the Comprehensive analysis server for the Mycobacterium t u b erculosis complex (CASTB)...
May 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28527287/-identification-of-the-mycobacterium-tuberculosis-beijing-lineage-in-ecuador
#17
Patricia Jiménez, Karina Calvopiña, Diana Herrera, Carlos Rojas, Laura Pérez-Lago, Marcelo Grijalva, Remedios Guna, Darío García-de Viedma
INTRODUCTION: Mycobacterium tuberculosis Beijing lineage isolates are considered to be especially virulent, transmissible and prone to acquire resistances. Beijing strains have been reported worldwide, but studies in Latin America are still scarce. The only multinational study performed in the region indicated a heterogeneous distribution for this lineage, which was absent in Chile, Colombia and Ecuador, although further studies found the lineage in Chile and Colombia. OBJECTIVE: To search for the presence of the Beijing lineage in Ecuador, the only country in the region where it remains unreported...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28506215/comparison-of-antibody-responses-against-mycobacterium-tuberculosis-antigen-rv0679c-in-tuberculosis-patients-from-the-endemic-and-non-endemic-regions-of-the-beijing-genotype-a-case-control-study
#18
COMPARATIVE STUDY
Jingge Zhao, Takashi Matsuba, Xiaoyan Zhang, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Elizabeth Freda Telan, Yasuhiko Suzuki, Toshio Hattori
BACKGROUND: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses...
May 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28482417/-molecular-epidemiologic-study-on-mycobacterium-tuberculosis-from-drug-resistance-monitoring-sites-of-guangdong-province-2015
#19
X C Huang, H X Guo, Z H Wu, C X Guo, W J Wei, H C Li, Q Sun, C C Zhang, Z Y Li, T Chen, Q Zhong, L Zhou
Objective: To understand the characteristics of Mycobacterium tuberculosis (MTB) in epidemiology and distribution from Guangdong Province, and to explore the risk factors associated with drug resistance. Methods: A total of 225 clinical strains of MTB collected from 5 drug resistance monitoring sites of Guangdong Province in 2015 were tested by Regions of Difference 105 (RD105) deletion test and 15 loci mycobacterial interspersed repetitive units (MIRU) were used for genotyping. Gene clustering was analyzed using BioNumerics7...
May 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28469152/lineage-specific-snps-for-genotyping-of-mycobacterium-tuberculosis-clinical-isolates
#20
Horng-Yunn Dou, Chien-Hsing Lin, Yih-Yuan Chen, Shiu-Ju Yang, Jia-Ru Chang, Keh-Ming Wu, Ying-Tsong Chen, Pei-Ju Chin, Yen-Ming Liu, Ih-Jen Su, Shih-Feng Tsai
Tuberculosis (TB) is a severe infectious disease worldwide. Genetic variation of the causative agent, Mycobacterium tuberculosis (MTB), determines the outcomes of infection and anti-TB treatment. Until recently, there has been no effective and convenient way for classifying clinical isolates based on the DNA sequences of the divergent lineages of MTB infecting human populations. Here, we identified single nucleotide polymorphisms (SNPs) of six representative strains from Taiwan by whole-genome sequencing and comparing the results to the sequence of the H37Rv reference strain...
May 3, 2017: Scientific Reports
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