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Beijing genotype and mycobacterium tuberculosis

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https://www.readbyqxmd.com/read/28745309/a-quantitative-and-efficient-approach-to-select-miru-vntr-loci-based-on-accumulation-of-the-percentage-differences-of-strains-for-discriminating-divergent-mycobacterium-tuberculosis-sublineages
#1
Xin-Ling Pan, Chun-Lei Zhang, Chie Nakajima, Jin Fu, Chang-Xia Shao, Li-Na Zhao, Jia-Yi Cui, Na Jiao, Chang-Long Fan, Yasuhiko Suzuki, Toshio Hattori, Di Li, Hong Ling
Although several optimal mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) loci have been suggested for genotyping homogenous Mycobacterium tuberculosis, including the Beijing genotype, a more efficient and convenient selection strategy for identifying optimal VNTR loci is needed. Here 281 M. tuberculosis isolates were analyzed. Beijing genotype and non-Beijing genotypes were identified, as well as Beijing sublineages, according to single nucleotide polymorphisms. A total of 22 MIRU-VNTR loci were used for genotyping...
July 26, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28734764/comparative-genomic-analysis-of-mycobacterium-tuberculosis-beijing-like-strains-revealed-specific-genetic-variations-associated-with-virulence-and-drug-resistance
#2
Juan Germán Rodríguez-Castillo, Camilo Pino, Luis Fernando Niño, Juan Carlos Rozo, Claudia Llerena-Polo, Carlos A Parra-López, Andreas Tauch, Martha Isabel Murcia-Aranguren
Isolates of the Mycobacterium tuberculosis lineage 2/East-Asian are considered one of the most successful strains due to their increased pathogenicity, hyper-virulence associated with drug resistance, and high transmission. Recent studies in Colombia have shown that the Beijing-like genotype is associated with multidrug-resistance and high prevalence in the southwest of the country, but the genetic basis of its success in dissemination is unknown. In contribution to this matter, we obtained the whole sequences of six genomes of clinical isolates assigned to the Beijing-like genotype...
July 19, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28710410/combining-molecular-typing-and-spatial-pattern-analysis-to-identify-areas-of-high-tuberculosis-transmission-in-a-moderate-incidence-county-in-taiwan
#3
Yih-Yuan Chen, Jia-Ru Chang, Chih-Da Wu, Yen-Po Yeh, Shiu-Ju Yang, Chih-Hao Hsu, Ming-Ching Lin, Ching-Fang Tsai, Ming-Shian Lin, Ih-Jen Su, Horng-Yunn Dou
In total, 303 randomly selected clinical Mycobacterium tuberculosis (MTB) isolates from 303 patients (collected January to December 2012) in central Taiwan were examined. The major lineages found were Beijing (N = 114, 37.62%), Haarlem (N = 76, 25.08%) and East African-Indian (EAI) (N = 42, 13.86%). Notably, younger persons (≤30 years old) were 6.58 times more likely to be infected with a Beijing genotype compared to older persons (>70 years) (p < 0.05). Combining molecular typing methods and geographical information system (GIS) analysis, we uncovered a twofold higher incidence of Beijing strains in a hotspot area (33%) compared to non-hotspot areas (17%)...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28684565/complete-genome-sequences-of-three-representative-mycobacterium-tuberculosis-beijing-family-strains-belonging-to-distinct-genotype-clusters-in-hanoi-vietnam-during-2007-to-2009
#4
Takayuki Wada, Minako Hijikata, Shinji Maeda, Nguyen Thi Le Hang, Pham Huu Thuong, Nguyen Phuong Hoang, Nguyen Van Hung, Naoto Keicho
We present here three complete genome sequences of Mycobacterium tuberculosis Beijing family strains isolated in Hanoi, Vietnam. These three strains were selected from major genotypic clusters (15-MIRU-VNTR) identified in a previous population-based study. We emphasize their importance and potential as reference strains in this Asian region.
July 6, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28659320/development-of-a-one-step-multiplex-pcr-assay-for-differential-detection-of-major-mycobacterium-species
#5
Hansong Chae, Seung Jung Han, Su Young Kim, Chang-Seok Ki, Hee Jae Huh, Dongeun Yong, Won-Jung Koh, Sung Jae Shin
The prevalence of tuberculosis continues to be high, and nontuberculous mycobacteria (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of this assay were to: 1) discriminate between the Mycobacterium tuberculosis complex (MTBC) and NTM using rv0577 or RD750; 2) differentiate M...
June 28, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28610791/the-role-of-the-time-kill-kinetics-assay-as-part-of-a-preclinical-modeling-framework-for-assessing-the-activity-of-anti-tuberculosis-drugs
#6
Hannelore I Bax, Irma A J M Bakker-Woudenberg, Corné P de Vogel, Aart van der Meijden, Annelies Verbon, Jurriaan E M de Steenwinkel
Novel treatment strategies for tuberculosis are urgently needed. Many different preclinical models assessing anti-tuberculosis drug activity are available, but it is yet unclear which combination of models is most predictive of clinical treatment efficacy. The aim of this study was to determine the role of our in vitro time kill-kinetics assay as an asset to a predictive preclinical modeling framework assessing anti-tuberculosis drug activity. The concentration- and time-dependent mycobacterial killing capacities of six anti-tuberculosis drugs were determined during exposure as single drugs or in dual, triple and quadruple combinations towards a Mycobacterium tuberculosis Beijing genotype strain and drug resistance was assessed...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28610789/comparative-study-of-genotypes-of-mycobacterium-tuberculosis-from-a-northern-indian-setting-with-strains-reported-from-other-parts-of-india-and-neighboring-countries
#7
Pragya Sharma, Kiran Katoch, Shilpi Chandra, Devendra Singh Chauhan, Vishnu Dutt Sharma, David Couvin, Nalin Rastogi, Vishwa Mohan Katoch
This study was carried out to characterize Mycobacterium tuberculosis population in Ghatampur, Kanpur, North India, by spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTRs) typing. A total of 335 isolates were genotyped by spoligotyping and Central Asian (CAS) sub-lineage was the most prevalent, comprising 59.1% of all isolates. Other lineages were: East-African Indian (EAI) (19.10%), T (5.07%), Beijing (3.28%), Manu (2.98%), X (2.68%), S (0.89%), H3 (0...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28596961/first-insight-into-the-molecular-epidemiology-of-mycobacterium-tuberculosis-isolates-from-the-minority-enclaves-of-southwestern-china
#8
Lianyong Chen, Yu Pang, Li Ma, Huijuan Yang, Haohao Ru, Xing Yang, Shuangqun Yan, Mao Jia, Lin Xu
Yunnan is a province located in southwestern China. The aim of this study was to investigate the genetic diversity of Mycobacterium tuberculosis (MTB) strains circulating in Yunnan Province. We used spoligotyping and a 12-locus high-resolution VNTR set to identify a total of 271 MTB isolates collected from six prefectures' Yunnan. All the 271 patients were classified as 11 different ethnic groups, including 133 Han patients (49.1%) and 138 minority patients (50.9%). Spoligotyping analyses revealed that the largest two spoligotypes were SIT1 (Beijing family, n = 136) and SIT53 (T family, n = 35)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28588286/mycobacterium-tuberculosis-beijing-genotype-family-strain-isolated-from-naturally-infected-plateau-zokor-myospalax-baileyi-in-china
#9
Lin Sun, Xiang Chen, Wenhai Zhang, Gencheng Huang, Yuanyuan Zhang, Zhengzhong Xu, Baofa Yin, Wanhong Wei, Xinan Jiao, Kanglin Wan
No abstract text is available yet for this article.
June 7, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28550826/-multidrug-resistant-tuberculosis-by-strains-of-beijing-family-in-patients-from-lisbon-portugal-preliminary-report
#10
Fernando Maltez, Teresa Martins, Diana Póvoas, João Cabo, Helena Peres, Francisco Antunes, João Perdigão, Isabel Portugal
INTRODUCTION: Beijing family strains of Mycobacterium tuberculosis are associated with multidrug-resistance. Although strains of the Lisboa family are the most common among multidrug-resistant and extensively drug-resistant patients in the region, several studies have reported the presence of the Beijing family. However, the features of patients from whom they were isolated, are not yet known. MATERIAL AND METHODS: Retrospective study involving 104 multidrug-resistant and extensively drug-resistant strains of Mycobacterium tuberculosis, from the same number of patients, isolated and genotyped between 1993 and 2015 in Lisbon...
March 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28545488/genetic-diversity-of-mycobacterium-tuberculosis-isolates-from-tochigi-prefecture-a-local-region-of-japan
#11
Fuminori Mizukoshi, Tohru Miyoshi-Akiyama, Hiroki Iwai, Takako Suzuki, Reiko Kiritani, Teruo Kirikae, Keiji Funatogawa
BACKGROUND: Foreign-born patients with tuberculosis (TB) may introduce globally disseminated isolates of Mycobacterium tuberculosis into large cities in Japan. The risk of dissemination of these isolates into local regions, however, has not been determined. This study analyzed the molecular epidemiology of M. tuberculosis isolates obtained from TB patients living in a local region of Japan. METHODS: Whole genome sequences of 169 M. tuberculosis isolates, obtained from 148 Japanese-born and 21 foreign-born patients living in Tochigi, Japan, were analyzed using the Comprehensive analysis server for the Mycobacterium t u b erculosis complex (CASTB)...
May 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28527287/-identification-of-the-mycobacterium-tuberculosis-beijing-lineage-in-ecuador
#12
Patricia Jiménez, Karina Calvopiña, Diana Herrera, Carlos Rojas, Laura Pérez-Lago, Marcelo Grijalva, Remedios Guna, Darío García-de Viedma
INTRODUCTION: Mycobacterium tuberculosis Beijing lineage isolates are considered to be especially virulent, transmissible and prone to acquire resistances. Beijing strains have been reported worldwide, but studies in Latin America are still scarce. The only multinational study performed in the region indicated a heterogeneous distribution for this lineage, which was absent in Chile, Colombia and Ecuador, although further studies found the lineage in Chile and Colombia. OBJECTIVE: To search for the presence of the Beijing lineage in Ecuador, the only country in the region where it remains unreported...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28506215/comparison-of-antibody-responses-against-mycobacterium-tuberculosis-antigen-rv0679c-in-tuberculosis-patients-from-the-endemic-and-non-endemic-regions-of-the-beijing-genotype-a-case-control-study
#13
Jingge Zhao, Takashi Matsuba, Xiaoyan Zhang, Susan Leano, Chie Nakajima, Haorile Chagan-Yasutan, Elizabeth Freda Telan, Yasuhiko Suzuki, Toshio Hattori
BACKGROUND: Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses...
May 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28482417/-molecular-epidemiologic-study-on-mycobacterium-tuberculosis-from-drug-resistance-monitoring-sites-of-guangdong-province-2015
#14
X C Huang, H X Guo, Z H Wu, C X Guo, W J Wei, H C Li, Q Sun, C C Zhang, Z Y Li, T Chen, Q Zhong, L Zhou
Objective: To understand the characteristics of Mycobacterium tuberculosis (MTB) in epidemiology and distribution from Guangdong Province, and to explore the risk factors associated with drug resistance. Methods: A total of 225 clinical strains of MTB collected from 5 drug resistance monitoring sites of Guangdong Province in 2015 were tested by Regions of Difference 105 (RD105) deletion test and 15 loci mycobacterial interspersed repetitive units (MIRU) were used for genotyping. Gene clustering was analyzed using BioNumerics7...
May 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28469152/lineage-specific-snps-for-genotyping-of-mycobacterium-tuberculosis-clinical-isolates
#15
Horng-Yunn Dou, Chien-Hsing Lin, Yih-Yuan Chen, Shiu-Ju Yang, Jia-Ru Chang, Keh-Ming Wu, Ying-Tsong Chen, Pei-Ju Chin, Yen-Ming Liu, Ih-Jen Su, Shih-Feng Tsai
Tuberculosis (TB) is a severe infectious disease worldwide. Genetic variation of the causative agent, Mycobacterium tuberculosis (MTB), determines the outcomes of infection and anti-TB treatment. Until recently, there has been no effective and convenient way for classifying clinical isolates based on the DNA sequences of the divergent lineages of MTB infecting human populations. Here, we identified single nucleotide polymorphisms (SNPs) of six representative strains from Taiwan by whole-genome sequencing and comparing the results to the sequence of the H37Rv reference strain...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28456705/positive-epistasis-of-major-low-cost-drug-resistance-mutations-rpob531-ttg-and-katg315-acc-depends-on-the-phylogenetic-background-of-mycobacterium-tuberculosis-strains
#16
Qin-Jing Li, Wei-Wei Jiao, Qing-Qin Yin, Ying-Jia Li, Jie-Qiong Li, Fang Xu, Lin Sun, Jing Xiao, Hui Qi, Ting Wang, Igor Mokrousov, Hai-Rong Huang, A-Dong Shen
Mycobacterium tuberculosis Beijing genotype strains increasingly circulate in different world regions, either as historical endemic, e.g. in East Asia, or recently imported, e.g. in South America, and this family is regarded as the most successful lineage of the global tuberculosis (TB) epidemic. Here we analysed the transmission capacity of these strains in the context of their phylogenetic background and drug resistance mutations. The study collection included all multidrug resistant (MDR) strains of Beijing genotype isolated in Beijing Chest Hospital, the largest tertiary TB facility in North China, in 2011-2013 (n = 278)...
June 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28404234/frequency-of-rrs-and-rpsl-mutations-in-streptomycin-resistant-mycobacterium-tuberculosis-isolates-from-iranian-patients
#17
Azar Dokht Khosravi, Nayereh Etemad, Mohammad Hashemzadeh, Solmaz Khandan Dezfuli, Hamed Goodarzi
OBJECTIVES: Streptomycin (SM) is one of the most effective drugs for the treatment of multidrug-resistant (MDR) tuberculosis. However, resistance to SM is increasingly reported, mainly due to mutations in the rpsL and rrs genes. This study was designed with the aim of determining the nature of SM resistance and the type and frequency of rpsL and rrs mutations among SM-resistant Mycobacterium tuberculosis (MTB) isolates from Iran. METHODS: A total of 100 clinical monoresistant and MDR MTB isolates were subjected to drug susceptibility testing (DST) for SM...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28397531/drug-resistance-in-newly-presenting-and-previously-treated-tuberculosis-patients-in-guangxi-province-people-s-republic-of-china
#18
Dan Luo, Jinming Zhao, Mei Lin, Feiying Liu, Shuhai Huang, Yingkun Zhang, Minying Huang, Juan Li, Yang Zhou, Rushu Lan, Yanlin Zhao
Drug-resistant Mycobacterium tuberculosis strains are a major threat to the control of tuberculosis (TB), but the prevalence of drug-resistant TB is still unknown in the southern ethnic region of China. A cluster-randomized sampling method was used to include the study population. Isolates were tested for resistance to 6 antituberculosis drugs, and genotyped to identify Beijing strains. Overall, 11.3% (139/1229) of new cases and 33.0% (126/382) of retreated cases had drug-resistant tuberculosis. Multiple previous TB treatment episodes and multiple treatment interruptions were risk factors for both drug-resistant and multidrug-resistant TB among retreated cases...
May 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28377903/the-bioinformatics-analysis-of-comparative-genomics-of-mycobacterium-tuberculosis-complex-mtbc-provides-insight-into-dissimilarities-between-intraspecific-groups-differing-in-host-association-virulence-and-epitope-diversity
#19
Xinmiao Jia, Li Yang, Mengxing Dong, Suting Chen, Lingna Lv, Dandan Cao, Jing Fu, Tingting Yang, Ju Zhang, Xiangli Zhang, Yuanyuan Shang, Guirong Wang, Yongjie Sheng, Hairong Huang, Fei Chen
Tuberculosis now exceeds HIV as the top infectious disease cause of mortality, and is caused by the Mycobacterium tuberculosis complex (MTBC). MTBC strains have highly conserved genome sequences (similarity >99%) but dramatically different phenotypes. To analyze the relationship between genotype and phenotype, we conducted the comparative genomic analysis on 12 MTBC strains representing different lineages (i.e., Mycobacterium bovis; M. bovis BCG; M. microti; M. africanum; M. tuberculosis H37Rv; M. tuberculosis H37Ra, and six M...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28374900/spoligotyping-of-mycobacterium-tuberculosis-isolates-at-a-tertiary-care-hospital-in-india
#20
Shirly Suzana, Sivakumar Shanmugam, K R Uma Devi, P N Swarna Latha, Joy S Michael
OBJECTIVE: Spoligotyping is a valuable genotyping tool to study the genetic diversity and molecular epidemiology of Mycobacterium tuberculosis (M. tb). The aim of this study was to analyse different spoligotype patterns of M. tb strains isolated from patients with tuberculosis from different parts of India. MATERIALS AND METHODS: A total of 163 M. tb isolates were spoligotyped between January 2014 and January 2015. About 47% (n = 77) were from patients with extrapulmonary tuberculosis; of these, 10 were MDR, and seven were Pre-XDR...
April 4, 2017: Tropical Medicine & International Health: TM & IH
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