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Beijing genotype and mycobacterium tuberculosis

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https://www.readbyqxmd.com/read/28404234/frequency-of-rrs-and-rpsl-mutations-in-streptomycin-resistant-mycobacterium-tuberculosis-isolates-from-iranian-patients
#1
Azar Dokht Khoravi, Nayereh Etemad, Mohammad Hashemzadeh, Solmaz Khandan Dezfuli, Hamed Goodarzi
OBJECTIVES: Streptomycin (SM) is one of the most effective drugs for the treatment of multidrug-resistant (MDR) tuberculosis. However, resistance to SM is increasingly reported, mainly due to mutations in the rpsL and rrs genes. This study was designed with the aim of determining the nature of SM resistance and the type and frequency of rpsL and rrs mutations among SM-resistant Mycobacterium tuberculosis (MTB) isolates from Iran. METHODS: A total of 100 clinical monoresistant and MDR MTB isolates were subjected to drug susceptibility testing (DST) for SM...
April 9, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28397531/drug-resistance-in-newly-presenting-and-previously-treated-tuberculosis-patients-in-guangxi-province-people-s-republic-of-china
#2
Dan Luo, Jinming Zhao, Mei Lin, Feiying Liu, Shuhai Huang, Yingkun Zhang, Minying Huang, Juan Li, Yang Zhou, Rushu Lan, Yanlin Zhao
Drug-resistant Mycobacterium tuberculosis strains are a major threat to the control of tuberculosis (TB), but the prevalence of drug-resistant TB is still unknown in the southern ethnic region of China. A cluster-randomized sampling method was used to include the study population. Isolates were tested for resistance to 6 antituberculosis drugs, and genotyped to identify Beijing strains. Overall, 11.3% (139/1229) of new cases and 33.0% (126/382) of retreated cases had drug-resistant tuberculosis. Multiple previous TB treatment episodes and multiple treatment interruptions were risk factors for both drug-resistant and multidrug-resistant TB among retreated cases...
April 1, 2017: Asia-Pacific Journal of Public Health
https://www.readbyqxmd.com/read/28377903/the-bioinformatics-analysis-of-comparative-genomics-of-mycobacterium-tuberculosis-complex-mtbc-provides-insight-into-dissimilarities-between-intraspecific-groups-differing-in-host-association-virulence-and-epitope-diversity
#3
Xinmiao Jia, Li Yang, Mengxing Dong, Suting Chen, Lingna Lv, Dandan Cao, Jing Fu, Tingting Yang, Ju Zhang, Xiangli Zhang, Yuanyuan Shang, Guirong Wang, Yongjie Sheng, Hairong Huang, Fei Chen
Tuberculosis now exceeds HIV as the top infectious disease cause of mortality, and is caused by the Mycobacterium tuberculosis complex (MTBC). MTBC strains have highly conserved genome sequences (similarity >99%) but dramatically different phenotypes. To analyze the relationship between genotype and phenotype, we conducted the comparative genomic analysis on 12 MTBC strains representing different lineages (i.e., Mycobacterium bovis; M. bovis BCG; M. microti; M. africanum; M. tuberculosis H37Rv; M. tuberculosis H37Ra, and six M...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28374900/spoligotyping-of-mycobacterium-tuberculosis-isolates-at-a-tertiary-care-hospital-in-india
#4
Suzana Shirly, Sivakumar Shanmugam, K R Uma Devi, P N Swarna Latha, Joy S Michael
OBJECTIVE: Spoligotyping is a valuable genotyping tool to study the genetic diversity and molecular epidemiology of Mycobacterium tuberculosis (M. tb). The aim of this study was to analyse different spoligotype patterns of M. tb strains isolated from tuberculosis patients from different parts of India. MATERIALS AND METHODS: 163 M. tb isolates were spoligotyped between January 2014 and January 2015. 47% (n=77) were from patients with extrapulmonary tuberculosis; of these, 10 were MDR and 7 were Pre-XDR...
April 4, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28337447/genotypic-diversity-of-mycobacterium-tuberculosis-clinical-isolates-in-the-multiethnic-area-of-the-xinjiang-uygur-autonomous-region-in-china
#5
Jie Liu, Junlian Li, Jiao Liu, Xiuqin Zhao, Lulu Lian, Haican Liu, Bing Lu, Qin Yu, Jingrui Zhang, Yingcheng Qi, Kanglin Wan
Objectives. We studied the genetic diversity of clinical isolates from patients with tuberculosis in the multiethnic area of Xinjiang autonomous region in China. A total of 311 clinical M. tuberculosis isolates were collected in 2006 and 2011 and genotyped by two genotyping methods. All isolates were grouped into 68 distinct spoligotypes using the spoligotyping method. The Beijing family was dominant, followed by T1 and CAS. MIRU-VNTR results showed that a total of 195 different VNTR types were identified. Ten of the 15 loci were highly or moderately discriminant according to their HGDI scores, and 13 loci had good discriminatory power in non-Beijing family strains, whereas only two loci had good discriminatory power in Beijing family strains...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28333978/association-between-genotype-and-drug-resistance-profiles-of-mycobacterium-tuberculosis-strains-circulating-in-china-in-a-national-drug-resistance-survey
#6
Yang Zhou, Susan van den Hof, Shengfen Wang, Yu Pang, Bing Zhao, Hui Xia, Richard Anthony, Xichao Ou, Qiang Li, Yang Zheng, Yuanyuan Song, Yanlin Zhao, Dick van Soolingen
We describe the population structure of a representative collection of 3,133 Mycobacterium tuberculosis isolates, collected within the framework of a national resistance survey from 2007 in China. Genotyping data indicate that the epidemic strains in China can be divided into seven major complexes, of which 92% belonged to the East Asian (mainly Beijing strains) or the Euro-American lineage. The epidemic Beijing strains in China are closely related to the Beijing B0/W148 strain earlier described in Russia and a large cluster of these strains has spread national wide...
2017: PloS One
https://www.readbyqxmd.com/read/28330178/emergence-of-mixed-infection-of-beijing-non-beijing-strains-among-multi-drug-resistant-mycobacterium-tuberculosis-in-pakistan
#7
Samar Mustafa, Hasnain Javed, Jawairia Hashmi, Nazia Jamil, Zarfishan Tahir, Abdul Majeed Akhtar
Tuberculosis (TB) remains as one of the deadliest diseases after HIV globally with 95 % of deaths confined to low-and-middle income countries. Pakistan is fifth among the 22 high-burden TB countries with the incidence rate of 230/100,000 persons, however, studies related to prevalent Mycobacterium tuberculosis strains and their spread, drug resistance pattern and evolutionary genetics are inadequate. The present study was undertaken to highlight the circulation of M. tuberculosis strains causing drug resistant TB in our community by targeting the molecular marker IS6110 and then characterization of these strains as Beijing and Non-Beijing genotypes...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28231674/-a-preliminary-study-on-the-molecular-characteristics-of-d-cycloserine-resistance-of-mycobacterium-tuberculosis
#8
C Li, G L Li, Q Luo, S J Li, R B Wang, Y L Lou, J X Lyu, K L Wan
Objective: To investigate the relationship between D-cycloserine resistance and the gene mutations of alrA, ddlA and cycA of Mycobacterium (M.) tuberculosis, as well as the association between D-cycloserine resistance and spoligotyping genotyping. Methods: A total of 145 M. tuberculosis strains were selected from the strain bank. D-cycloserine resistant phenotypes of the strains were determined by the proportion method and the minimal inhibitory concentration was determined by resazurin microtiter assay. PCR amplification and DNA direct sequencing methods were used for the analysis of gene mutations...
February 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28223967/secretome-prediction-of-two-m-tuberculosis-clinical-isolates-reveals-their-high-antigenic-density-and-potential-drug-targets
#9
Fernanda Cornejo-Granados, Zyanya L Zatarain-Barrón, Vito A Cantu-Robles, Alfredo Mendoza-Vargas, Camilo Molina-Romero, Filiberto Sánchez, Luis Del Pozo-Yauner, Rogelio Hernández-Pando, Adrián Ochoa-Leyva
The Excreted/Secreted (ES) proteins play important roles during Mycobacterium tuberculosis invasion, virulence, and survival inside the host and they are a major source of immunogenic proteins. However, the molecular complexity of the bacillus cell wall has made difficult the experimental isolation of the total bacterial ES proteins. Here, we reported the genomes of two Beijing genotype M. tuberculosis clinical isolates obtained from patients from Vietnam (isolate 46) and South Africa (isolate 48). We developed a bioinformatics pipeline to predict their secretomes and observed that ~12% of the genome-encoded proteins are ES, being PE, PE-PGRS, and PPE the most abundant protein domains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28222189/molecular-epidemiology-of-mycobacterium-tuberculosis-complex-in-brussels-2010-2013
#10
Christelle Vluggen, Karine Soetaert, Guido Groenen, Maryse Wanlin, Martine Spitaels, Wouter Arrazola de Oñate, Maryse Fauville-Dufaux, Claude Saegerman, Vanessa Mathys
The tuberculosis (TB) incidence rate in Brussels-Capital Region is 3-fold higher than in Belgium as a whole. Eight years after the realization of initial prospective population-based molecular epidemiology investigations in this Region, a similar study over the period 2010-2013 was conducted. TB strains isolated from 945 patients were submitted to genotyping by standardized 24-locus-MIRU-VNTR typing and spoligotyping. The phylogenetic analysis showed that the LAM (16.7%) and Haarlem (15.7%) branches are the two most prevalent TB lineages circulating in Brussels...
2017: PloS One
https://www.readbyqxmd.com/read/28214557/quadruple-first-line-drug-resistance-in-mycobacterium-tuberculosis-in-vietnam-what-can-we-learn-from-genes
#11
Huy Quang Nguyen, Nhung Viet Nguyen, Lucie Contamin, Thanh Hoa Thi Tran, Thuong Thi Vu, Hung Van Nguyen, Ngoc Lan Thi Nguyen, Son Thai Nguyen, Anh Duc Dang, Anne-Laure Bañuls, Van Anh Thi Nguyen
In Vietnam, a country with high tuberculosis (137/100.000 population) and multidrug-resistant (MDR)-TB burdens (7.8/100.000 population), little is known about the molecular signatures of drug resistance in general and more particularly of second line drug (SLD) resistance. This study is specifically focused on Mycobacterium tuberculosis isolates resistant to four first-line drugs (FLDs) that make TB much more difficult to treat. The aim is to determine the proportion of SLD resistance in these quadruple drug resistant isolates and the genetic determinants linked to drug resistance to better understand the genetic processes leading to quadruple and extremely drug resistance (XDR)...
February 21, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28201993/structure-and-variation-of-crispr-and-crispr-flanking-regions-in-deleted-direct-repeat-region-mycobacterium-tuberculosis-complex-strains
#12
Paul Jeffrey Freidlin, Israel Nissan, Anna Luria, Drora Goldblatt, Lana Schaffer, Hasia Kaidar-Shwartz, Daniel Chemtob, Zeev Dveyrin, Steven Robert Head, Efrat Rorman
BACKGROUND: CRISPR and CRISPR-flanking genomic regions are important for molecular epidemiology of Mycobacterium tuberculosis complex (MTBC) strains, and potentially for adaptive immunity to phage and plasmid DNA, and endogenous roles in the bacterium. Genotyping in the Israel National Mycobacterium Reference Center Tel-Aviv of over 1500 MTBC strains from 2008-2013 showed three strains with validated negative 43-spacer spoligotypes, that is, with putatively deleted direct repeat regions (deleted-DR/CRISPR regions)...
February 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28182078/difference-in-antibody-responses-to-mycobacterium-tuberculosis-antigens-in-japanese-tuberculosis-patients-infected-with-the-beijing-non-beijing-genotype
#13
Jingge Zhao, Beata Shiratori, Masao Okumura, Hideki Yanai, Makoto Matsumoto, Chie Nakajima, Kazue Mizuno, Kenji Ono, Tetsuya Oda, Haorile Chagan-Yasutan, Yugo Ashino, Takashi Matsuba, Takashi Yoshiyama, Yasuhiko Suzuki, Toshio Hattori
The Beijing genotype Mycobacterium tuberculosis (MTB), notorious for its virulence and predisposition to relapse, could be identified by spoligotyping based on genetic heterogeneity. The plasma samples from 20 cases of Beijing and 16 cases of non-Beijing MTB infected individuals and 24 healthy controls (HCs) were collected, and antibodies against 11 antigens (Rv0679c142Asn, Rv0679c142Lys, Ag85B, Ag85A, ARC, TDM-M, TDM-K, HBHA, MDP-1, LAM, and TBGL) were measured by ELISA. Compared to the HCs, the MTB infected subjects showed higher titers of anti-Ag85B IgG (positivity 58...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28043622/emerging-clones-of-mycobacterium-tuberculosis-in-russia-and-former-soviet-union-countries-beijing-genotype-and-beyond
#14
Igor Mokrousov
No abstract text is available yet for this article.
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043579/deletion-of-region-of-difference-181-in-mycobacterium-tuberculosis-beijing-strains
#15
Ehsan Sharifipour, Parissa Farnia, Mohadeseh Mozafari, Shiva Irani, Ali Akbar Velayati
OBJECTIVES/BACKGROUND: The region of differences (RDs) polymorphisms is a potential molecular epidemiology method to distinguish origins of Mycobacterium tuberculosis. To date, 68 RDs have been identified in M. tuberculosis. This study was designed to determine the frequency of RD deletions in M. tuberculosis strains that were isolated from patients with pulmonary tuberculosis who were referred to the National Research Institute of Tuberculosis and Lung Disease for diagnosis and treatment...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043550/predominance-of-beijing-lineage-among-pre-extensively-drug-resistant-and-extensively-drug-resistant-strains-of-mycobacterium-tuberculosis-a-tertiary-care-center-experience
#16
Syed Beenish Rufai, Manimuthu Mani Sankar, Jitendra Singh, Sarman Singh
OBJECTIVE/BACKGROUND: Increasing resistance to various first-line and second-line drugs has become a major concern in India. However, it is not known if some genotypes are more associated with second-line drug resistance. Thus, the main aim of this study was to find out the predominant genotype associated with second-line drug resistance. METHODS: During the study, a total of 234 multidrug resistant (MDR) strains of Mycobacterium tuberculosis, isolated between 2008 and 2015, were randomly selected and screened for pre-extensively drug-resistant (XDR) and XDR patterns using second-drug susceptibility testing with BACTEC MGIT 960...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043502/genotypic-analysis-of-multidrug-resistant-tuberculosis-isolates-from-extra-pulmonary-tuberculosis-cases-in-tertiary-care-centers-in-northern-india
#17
Anand K Maurya, Vijaya L Nag, Surya Kant, Ramawadh S Kushwaha, Tapan N Dhole
OBJECTIVE/BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem. The diagnosis of MDR-TB is of paramount importance in establishing appropriate clinical management and infection control measures. Rapid detection of MDR-TB allows the establishment of an effective treatment regimen, minimizes the risk of further resistance, and limits the spread of drug-resistant strains. The aim of this study is to determine the genotypic characterization of MDR-TB isolates from extra pulmonary tuberculosis (EPTB) cases in tertiary care centers in Northern India...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043501/comparative-proteomic-analysis-of-sequential-isolates-of-mycobacterium-tuberculosis-sensitive-and-resistant-beijing-type-from-a-patient-with-pulmonary-tuberculosis
#18
Amit Singh, Anil Kumar Gupta, Krishnamoorthy Gopinath, Divakar Sharma, Prashant Sharma, Deepa Bisht, Pawan Sharma, Sarman Singh
AIM & OBJECTIVE: In India, tuberculosis (TB) is a foremost health problem, and the emergence of multidrug-resistant (MDR) and extensively drug resistant (XDR) strains of Mycobacterium tuberculosis (M. tuberculosis) has further complicated the situation. Although various mechanisms have been proposed to elucidate the emergence of resistance, our knowledge remains insufficient. The formation of a very complex network and drugs of proteins are countered by their efflux/modification or target over-expression/modification...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27995145/a-first-insight-into-the-genetic-diversity-and-drug-susceptibility-pattern-of-mycobacterium-tuberculosis-complex-in-zhejiang-china
#19
Zhengwei Liu, Yu Pang, Songhua Chen, Beibei Wu, Haibo He, Aizhen Pan, Xiaomeng Wang
In this study, our aim was to determine the predominant genotypes among the Mycobacterium tuberculosis (MTB) strains circulating in Zhejiang Province. In addition, we also sought to determine the potential associations between MTB genotypes and susceptibility to first-line drugs. Out of these isolates, 673 (71.6%) were classified into the Beijing genotype, while the other 267 (28.4%) were from non-Beijing families. The highest proportion of Beijing genotype was found in Huzhou (80.0%) and the lowest in Lishui (48...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27919887/genotyping-and-prevalence-of-pyrazinamide-and-moxifloxacin-resistant-tuberculosis-in-china-2000-to-2010
#20
Yu Pang, Zhijian Zhang, Yufeng Wang, Shengfen Wang, Yuanyuan Song, Bing Zhao, Yang Zhou, Xichao Ou, Qiang Li, Hui Xia, Yang Zheng, Xiangqun Fang, Yanlin Zhao
We investigated the prevalence, trends, and risk factors for pyrazinamide (PZA) and moxifloxacin (MOX) resistance among tuberculosis (TB) cases in China and also analyzed the population structure of Mycobacterium tuberculosis strains. All the M. tuberculosis strains enrolled in this study were collected from the national TB prevalence surveys. Each strain was genotyped by analyzing the regions of RD105 and IS6110 in the NTF region. The Bactec MGIT 960 system was used to detect the drug susceptibility of M. tuberculosis isolates to PZA and MOX...
February 2017: Antimicrobial Agents and Chemotherapy
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