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Beijing genotype and mycobacterium tuberculosis

Ulziijargal Gurjav, Alexander C Outhred, Peter Jelfs, Nadine McCallum, Qinning Wang, Grant A Hill-Cawthorne, Ben J Marais, Vitali Sintchenko
Australia has a low tuberculosis incidence rate with most cases occurring among recent immigrants. Given suboptimal cluster resolution achieved with 24-locus mycobacterium interspersed repetitive unit (MIRU-24) genotyping, the added value of whole genome sequencing was explored. MIRU-24 profiles of all Mycobacterium tuberculosis culture-confirmed tuberculosis cases diagnosed between 2009 and 2013 in New South Wales (NSW), Australia, were examined and clusters identified. The relatedness of cases within the largest MIRU-24 clusters was assessed using whole genome sequencing and phylogenetic analyses...
2016: PloS One
Liqun Zhang, Yu Pang, Xia Yu, Yufeng Wang, Jie Lu, Mengqiu Gao, Hairong Huang, Yanlin Zhao
Pulmonary cavitation is one of the most frequently observed clinical characteristics in tuberculosis (TB). The objective of this study was to investigate the potential risk factors associated with cavitary TB in China. A total of 385 smear-positive patients were enrolled in the study, including 192 (49.9%) patients with cavitation as determined by radiographic findings. Statistical analysis revealed that the distribution of patients with diabetes in the cavitary group was significantly higher than that in the non-cavitary group (adjusted odds ratio (OR) (95% confidence interval (CI)):12...
October 12, 2016: Emerging Microbes & Infections
Maxime Barbier, Thierry Wirth
With the advent of next-generation sequencing technology, the genotyping of clinical Mycobacterium tuberculosis strains went through a major breakup that dramatically improved the field of molecular epidemiology but also revolutionized our deep understanding of the M. tuberculosis complex evolutionary history. The intricate paths of the pathogen and its human host are reflected by a common geographical origin in Africa and strong biogeographical associations that largely reflect the past migration waves out of Africa...
August 2016: Microbiology Spectrum
Qing-Qin Yin, Hai-Can Liu, Wei-Wei Jiao, Qin-Jing Li, Rui Han, Jian-Ling Tian, Zhi-Guang Liu, Xiu-Qin Zhao, Ying-Jia Li, Kang-Lin Wan, A-Dong Shen, Igor Mokrousov
Mycobacterium tuberculosis Beijing genotype originated in China and has undergone a dramatic population growth and global spread in the last century. Here, a collection of M. tuberculosis Beijing family isolates from different provinces across all China was genotyped by high-resolution (24-MIRU-VNTR) and low-resolution, high-rank (modern and ancient sublineages) markers. The molecular profiles and global and local phylogenies were compared to the strain phenotype and patient data. The phylogeographic patterns observed in the studied collection demonstrate that large-scale (but not middle/small-scale) distance remains one of the decisive factors of the genetic divergence of M...
September 29, 2016: Scientific Reports
Yanan Li, Yuling Wang, Zhi Zhang, Huixia Gao, Haibin Wang, Jinfeng Cao, Shumin Zhang, Yuzhen Liu, Jianhua Lu, Zungui Xu, Erhei Dai
Ethambutol (EMB) is an essential first-line drug for tuberculosis (TB) treatment. Nucleotide substitutions at embB codon 306 have been proposed as a potential marker for EMB resistance and a predictor for broad drug resistance in clinical Mycobacterium tuberculosis (MTB) isolates. However, discordant findings about the association between embB306 mutation and EMB resistance were reported. The Hebei province is located in the Beijing-Tianjin-Hebei integration region; however, little information about the genetic diversity of embB locus is available in this area...
September 26, 2016: Antimicrobial Agents and Chemotherapy
Jeroen de Keijzer, Arnout Mulder, Arnoud H de Ru, Dick van Soolingen, Peter A van Veelen
: The spread of multidrug resistant Mycobacterium tuberculosis is one of the major challenges in tuberculosis control. In Eurasia, the spread of multidrug resistant tuberculosis is driven by the M. tuberculosis Beijing genotype. In this study, we examined whether selective advantages are present in the proteome of Beijing isolates that contribute to the emergence of this genotype. To this end, we compared the proteome of M. tuberculosis Beijing to that of M. tuberculosis H37Rv, both in the presence and absence of the first-line antibiotic rifampicin...
August 26, 2016: Journal of Proteomics
Mandira Varma-Basil, Anshika Narang, Soumitesh Chakravorty, Kushal Garima, Shraddha Gupta, Naresh Kumar Sharma, Astha Giri, Thierry Zozio, David Couvin, Mahmud Hanif, Anuj Bhatnagar, Balakrishnan Menon, Stefan Niemann, Nalin Rastogi, David Alland, Mridula Bose
Several attempts have been made to associate phylogenetic differences among Mycobacterium tuberculosis strains to variations in the clinical outcome of the disease and to drug resistance. We genotyped 139 clinical isolates of M. tuberculosis obtained from patients of pulmonary tuberculosis in North Delhi region. The isolates were analyzed using nine Single nucleotide polymorphism (SNP) markers, spoligotyping and MIRU-VNTRs; and the results were correlated with their drug susceptibility profile. Results of SNP cluster group (SCG) analysis (available for 138 isolates) showed that the most predominant cluster was SCG 3a, observed in 58...
September 2016: Tuberculosis
Nontobeko Eunice Mvubu, Balakrishna Pillay, Junaid Gamieldien, William Bishai, Manormoney Pillay
Although pulmonary epithelial cells are integral to innate and adaptive immune responses during Mycobacterium tuberculosis infection, global transcriptomic changes in these cells remain largely unknown. Changes in gene expression induced in pulmonary epithelial cells infected with M. tuberculosis F15/LAM4/KZN, F11, F28, Beijing and Unique genotypes were investigated by RNA sequencing (RNA-Seq). The Illumina HiSeq 2000 platform generated 50 bp reads that were mapped to the human genome (Hg19) using Tophat (2...
December 2016: Developmental and Comparative Immunology
Jitendra Prasad Mathuria, Govind Narayan Srivastava, Pragya Sharma, Bharat Lal Mathuria, Sanjay Ojha, Vishwa Mohan Katoch, Shampa Anupurba
The global presence and rapid dissemination of Beijing genotype of Mycobacterium tuberculosis, makes it an important issue of public health. Its presence and association with multi-drug resistance has been shown in many settings. In present study we tried to find its prevalence and association with drug resistance in North India. One hundred and twenty four M. tuberculosis isolates were analyzed with spoligotyping, further drug susceptibility testing was done by 1% proportional method. Out of these, 11 (8.9%) M...
August 2, 2016: Journal of Infection and Public Health
Sharereh Khanipour, Nayereh Ebrahimzadeh, Morteza Masoumi, Fatemeh Sakhaei, Farhad Alinezhad, Elham Safarpour, Abolfazl Fateh, Ali Nour Nematollahi, Alireza Hadizadeh Tasbiti, Mohammad Reza Zolfaghari, Ahmad Reza Bahrmand, Mehdi Mirsaeidi, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat
The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing...
June 2016: Journal of Global Antimicrobial Resistance
Nat Smittipat, Tada Juthayothin, Pamaree Billamas, Sarinya Jaitrong, Kamolchanok Rukseree, Kanchana Dokladda, Boonchai Chaiyasirinroje, Areeya Disratthakit, Angkana Chaiprasert, Surakameth Mahasirimongkol, Hideki Yanai, Norio Yamada, Katsushi Tokunaga, Prasit Palittapongarnpim
The objectives of this study were to characterise mutations in rrs, rpsL and gidB genes in Mycobacterium tuberculosis isolates from Thailand and to examine possible associations between mutations and strain genotypes. In total, 110 streptomycin (STR)-resistant M. tuberculosis isolates and 51 STR-susceptible isolates obtained from a sample collection in Thailand during 1999-2011 were sequenced for mutation analysis in rrs, rpsL and gidB. Genotypes of the isolates were identified using spoligotyping and large sequence polymorphisms...
March 2016: Journal of Global Antimicrobial Resistance
Faranak Rezaei, Mehri Haeili, Abbasali Imani Fooladi, Gholam Ali Azari Garmjan, Mohammad Mehdi Feizabadi
Point mutations in the rpsL and rrs genes can lead to development of streptomycin (STR) resistance in Mycobacterium tuberculosis. The aims of this study were to determine the frequency of mutations in STR resistant M. tuberculosis isolates in Iran and to analyze the possible relationship between bacterial genotype and STR resistance. Twenty-three M. tuberculosis samples comprising 9 multidrug-resistant (MDR) and 14 non-MDR isolates, recovered from TB patients in four regions: Tehran (n = 14), Isfahan (n = 2), Zahedan (n = 2), and Khorasan (n = 5), were analysed...
July 5, 2016: Journal of Chemotherapy
Parul Singhal, Pratima Dixit, Pooja Singh, Indu Jaiswal, Mastan Singh, Amita Jain
BACKGROUND & OBJECTIVES: Pre-extensively drug resistant (pre-XDR) and extensively drug resistant tuberculosis (XDR-TB) have been areas of growing concern, and are posing threat to global efforts of TB control. The present study was planned to study the presence of pre-XDR and XDR Mycobacterium tuberculosis and their genotypes in clinical isolates obtained from previously treated cases of pulmonary TB. METHODS: A total of 219 isolates obtained from previously treated cases of pulmonary TB were subjected to first-line (streptomycin, isoniazid, rifampicin and ethambutol) and second-line (ofloxacin, kanamycin, capreomycin and amikacin) drug susceptibility testing on solid Lowenstein-Jensen medium by proportion method...
March 2016: Indian Journal of Medical Research
Yuhki Nagai, Yoshito Iwade, Manabu Nakano, Shigehiro Akachi, Takashi Kobayashi, Takamichi Nishinaka
Beijing genotype strains of Mycobacterium tuberculosis are geographically widespread and pose a notorious public health problem, these strains causing outbreaks of multidrug-resistant tuberculosis (TB); some studies have reported an association with drug resistance. Because the prevalence of Beijing strain has a substantial impact on TB control programs, the availability of a rapid and reliable method for detecting these strains is important for epidemiological monitoring of their circulation. The main methods currently used to identify Beijing genotype strains are IS6110 DNA fingerprinting, spoligotyping and PCR to detect specific deletions such as region of difference (RD)207...
July 2016: Microbiology and Immunology
Seifu Gizaw Feyisa, Mehri Haeili, Fatemeh Zahednamazi, Nader Mosavari, Mohammad Mohammad Taheri, Gholamreza Hamzehloo, Samin Zamani, Mohammad Mehdi Feizabadi
INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing ≥ 80% similarity by RFLP analysis...
April 2016: Revista da Sociedade Brasileira de Medicina Tropical
Takashi Matsuba, Umme Ruman Siddiqi, Toshio Hattori, Chie Nakajima, Jun Fujii, Yasuhiko Suzuki
The Mycobacterium tuberculosis Rv0679c protein is a surface protein that contributes to host cell invasion. We previously showed that a single nucleotide transition of the Rv0679c gene leads to a single amino acid substitution from asparagine to lysine at codon 142 in the Beijing genotype family. In this study, we examined the immunological effect of this substitution. Several recombinant proteins were expressed in Escherichia coli and Mycobacterium smegmatis and characterized with antisera and two monoclonal antibodies named 5D4-C2 and 8G10-H2...
May 2016: FEMS Microbiology Letters
A A Vyazovaya, I V Mokrousov, V Yu Zhuravlev, N S Solovieva, T F Otten, O A Manicheva, B I Vishnevsky, O V Narvskaya
The goal of this work was to study the genotypic characteristics of the multidrug-resistant (MDR, i.e., resistant to at least rifampicine and isoniazid) Mycobacterium tuberculosis strains isolated in 2011-2012 from tuberculosis (TB) patients in the Northwest Russia. Spoligotyping of 195 M. tuberculosis isolates identified 14 different spoligotypes and assigned isolates to the genetic families Beijing (n = 162, 83%), LAM (n = 15), H3/URAL (n = 14), as well as T, Haarlem and X. Spoligotypes SIT1 (Beijing), SIT42 (LAM) and SIT262 (H3/URAL) were the most prevalent...
2016: Molekuliarnaia Genetika, Mikrobiologiia i Virusologiia
Abubakar S Hoza, Sayoki G Mfinanga, Irmgard Moser, Brigitte König
Molecular typing of Mycobacterium tuberculosis(MTB) has greatly enhanced the understanding of the population structure of MTB isolates and epidemiology of tuberculosis (TB). To characterize prevalent genotypes of MTB, microarrays‑based spoligotyping and mycobacterial interspersed repetitive unit‑variable number of tandem repeats (MIRU‑VNTR) were applied on 80 isolates collected from primary health care facilities in Tanga, North‑eastern Tanzania. A total of 18 distinct spoligotypes were identified. The lineages by order of their predominance were EAI and CAS families (26...
May 2016: Tuberculosis
Yun-Gyoung Hur, Wou Young Chung, Ahreum Kim, Young Sun Kim, Hyon-Suk Kim, Sun-Hee Jang, Yeun Kim, Hyeyoung Lee, Kwang Joo Park, Sang-Nae Cho
OBJECTIVES: Beijing strain of Mycobacterium tuberculosis (M. tb) is characterized by a high incidence of transmission, relapse, and drug resistance. This study aimed to determine host immune responses to antigens derived from the Beijing/K strain which has been highly prevalent in tuberculosis (TB) outbreaks in South Korea. METHODS: We recruited 52 TB patients and 96 healthy subjects comprising 31 individuals with latent TB infection (LTBI) and 65 TB-naïve controls based on QuantiFERON-TB Gold In-Tube (QFT-IT) tests...
July 2016: Journal of Infection
S K Dhatwalia, R Yadav, D Behera, S M Rudramurthy, H Kaur, S Sethi
SETTING: A tertiary care hospital in North India. OBJECTIVES: To determine the prevalence of different genotypes and examine their association with drug resistance among clinical isolates of Mycobacterium tuberculosis from the northern region of India. DESIGN: We analysed 100 clinical isolates of M. tuberculosis using mycobacterial interspersed repetitive units genotyping and TbD1 analysis. RESULTS: The analysis revealed that 34% of strains belonged to the Delhi/CAS (TbD1-) lineage, 32% had unknown patterns (27 TbD1-, 5 TbD1+), 18% were of Beijing genotype (TbD1-) and 11% were of EAI lineages (TbD1+)...
June 2016: International Journal of Tuberculosis and Lung Disease
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