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genotyping dengue 2

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https://www.readbyqxmd.com/read/29792991/codon-adaptation-biases-among-sylvatic-and-urban-genotypes-of-dengue-virus-type-2
#1
Marielton Dos Passos Cunha, Ayda Susana Ortiz-Baez, Caio César de Melo Freire, Paolo Marinho de Andrade Zanotto
Dengue virus (DENV) emerged from the sylvatic environment and colonized urban settings, being sustained in a human-Aedes-human transmission chain, mainly by the bites of females of the anthropophilic species Aedes aegypti. Herein, we sought evidence for fine-tuning in viral codon usage, possibly due to viral adaptation to human transmission. We compared the codon adaptation of DENV serotype 2 (DENV-2) genotypes from urban and sylvatic habitats and tried to correlate the findings with key evolutionary determinants...
May 21, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29745863/generation-and-comparative-genomics-of-synthetic-dengue-viruses
#2
Eli Goz, Yael Tsalenchuck, Rony Oren Benaroya, Zohar Zafrir, Shimshi Atar, Tahel Altman, Justin Julander, Tamir Tuller
BACKGROUND: Synthetic virology is an important multidisciplinary scientific field, with emerging applications in biotechnology and medicine, aiming at developing methods to generate and engineer synthetic viruses. In particular, many of the RNA viruses, including among others the Dengue and Zika, are widespread pathogens of significant importance to human health. The ability to design and synthesize such viruses may contribute to exploring novel approaches for developing vaccines and virus based therapies...
May 8, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29735824/molecular-characterisation-and-phylogenetic-analysis-of-dengue-outbreak-in-pasighat-arunachal-pradesh-northeast-india
#3
Biswajyoti Borkakoty, Mandakini Das, Kishore Sarma, Aniruddha Jakharia, Palash Kumar Das, Chandrakanta Bhattacharya, Basumoti Apum, Dipankar Biswas
Background and Objectives: Dengue is one of the most prevalent arboviral diseases in the world with 390 million dengue infections per year. In this study, we report the molecular characterisation of dengue outbreak in Pasighat, Arunachal Pradesh, Northeast India during 2015. Subjects and Methods: : A total of 613 dengue-suspected cases were screened for dengue virus by dengue NS1 Ag and anti-dengue IgM antibody depending on the duration of sample collection and onset of symptom...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29666658/phylogenetic-analysis-of-dengue-virus-in-bangkalan-madura-island-east-java-province-indonesia
#4
Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29650565/complete-coding-sequences-of-dengue-virus-type-2-strains-from-febrile-patients-seen-in-malindi-district-hospital-kenya-during-the-2017-dengue-fever-outbreak
#5
Kimita Gathii, Josphat N Nyataya, Beth K Mutai, George Awinda, John N Waitumbi
We report here 10 complete polyprotein-coding sequences of dengue virus type 2 strains isolated from febrile patients who presented at Malindi District Hospital, Kenya, during a recent dengue fever outbreak. Phylogenetically, all the strains belonged to clonal serotype 2 of the Cosmopolitan genotype.
April 12, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29649118/optical-dna-biosensor-based-on-square-planar-ethyl-piperidine-substituted-nickel-ii-salphen-complex-for-dengue-virus-detection
#6
Eda Yuhana Ariffin, Ling Ling Tan, Nurul Huda Abd Karim, Lee Yook Heng
A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N , N '-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix...
April 12, 2018: Sensors
https://www.readbyqxmd.com/read/29621534/diversity-of-dengue-virus-3-genotype-iii-in-jeddah-saudi-arabia
#7
Anwar M Hashem, Sayed S Sohrab, Sherif A El-Kafrawy, Adly M M Abd-Alla, Saeid Abo El-Ela, Turki S Abujamel, Ahmed M Hassan, Suha A Farraj, Noura A Othman, Remi N Charrel, Esam I Azhar
BACKGROUND: Dengue is the most important arboviral disease in tropical and subtropical countries. Dispersal of the vector and an increase in migratory flow between countries have led to large epidemics and severe clinical outcomes. Over the past 20 years, dengue epidemics have become more wide-spread and frequent. Previous studies have shown that dengue is endemic in Jeddah, Makkah and Al-Madinah in western Saudi Arabia as well as in Jazan region in the southern part of the country. The four serotypes of dengue virus (DENV) have been reported from western Saudi Arabia...
April 2, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29587780/genotype-specific-and-cross-reactive-neutralizing-antibodies-induced-by-dengue-virus-infection-detection-of-antibodies-with-different-levels-of-neutralizing-activities-against-homologous-and-heterologous-genotypes-of-dengue-virus-type-2-in-common-marmosets
#8
Nor Azila Muhammad Azami, Meng Ling Moi, Yasushi Ami, Yuriko Suzaki, Chang-Kweng Lim, Satoshi Taniguchi, Masayuki Saijo, Tomohiko Takasaki, Ichiro Kurane
BACKGROUND: A vaccine against all four dengue virus (DENV) serotypes includes the formulation of one genotype of each serotype. Although genetic similarities among genotypes within a serotype are higher as compared to those among serotypes, differences in the immunogenicity of the included genotypes would be a critical issue in maximizing successful dengue vaccine development. Thus, we determined the neutralizing antibody responses against three genotypes of dengue virus serotype 2 (DENV-2), namely Cosmopolitan, Asian I, and Asian/American, after primary and secondary inoculation with DENV-2 in a dengue animal model, the common marmoset (Callithrix jacchus)...
March 27, 2018: Virology Journal
https://www.readbyqxmd.com/read/29559699/characterization-of-a-murine-model-of-non-lethal-symptomatic-dengue-virus-infection
#9
Vanessa V Sarathy, Mellodee White, Li Li, Jaclyn A Kaiser, Gerald A Campbell, Gregg N Milligan, Nigel Bourne, Alan D T Barrett
The mosquito-borne disease dengue is caused by four serologically- and genetically-related viruses, termed DENV-1 to DENV-4. Historical setbacks due to lack of human-like mouse models of dengue were partially remedied with characterization of lethal DENV-2 infection in immunocompromised AG129 mice (deficient in IFN-α/β/γ receptors). Recently, our group established lethal AG129 mouse infection models of DENV-1, DENV-3, and DENV-4 using human isolates. Here we compare a non-lethal, disseminated model of DENV-3 infection using strain D83-144 to that of the lethal outcome following infection by strain C0360/94...
March 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29557338/dengue-virus-infection-in-aedes-albopictus-during-the-2014-autochthonous-dengue-outbreak-in-tokyo-metropolis-japan
#10
Daisuke Kobayashi, Katsunori Murota, Ryosuke Fujita, Kentaro Itokawa, Akira Kotaki, Meng Ling Moi, Hiroko Ejiri, Yoshihide Maekawa, Kohei Ogawa, Yoshio Tsuda, Toshinori Sasaki, Mutsuo Kobayashi, Tomohiko Takasaki, Haruhiko Isawa, Kyoko Sawabe
In 2014 in Japan, 162 autochthonous dengue cases were reported for the first time in nearly 70 years. Here, we report the results of the detection and isolation of dengue virus (DENV) from mosquitoes collected in Tokyo Metropolis in 2014 and 2015. The phylogenetic relationship among DENV isolates from mosquitoes and from patients based on both the entire envelope gene and whole coding sequences was evaluated. Herein, 2,298 female and 956 male Aedes albopictus mosquitoes were collected at six suspected locations of DENV infection in Tokyo Metropolis from August to October in 2014 and grouped into 124 and 35 pools, respectively, for viral genome detection and DENV isolation...
May 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29494580/hyperendemic-dengue-transmission-and-identification-of-a-locally-evolved-denv-3-lineage-papua-new-guinea-2007-2010
#11
Dagwin Luang-Suarkia, Oriol Mitja, Timo Ernst, Shannon Bennett, Alfred Tay, Russell Hays, David W Smith, Allison Imrie
BACKGROUND: Dengue is endemic in the Western Pacific and Oceania and the region reports more than 200,000 cases annually. Outbreaks of dengue and severe dengue occur regularly and movement of virus throughout the region has been reported. Disease surveillance systems, however, in many areas are not fully established and dengue incidence is underreported. Dengue epidemiology is likely least understood in Papua New Guinea (PNG), where the prototype DENV-2 strain New Guinea C was first isolated by Sabin in 1944 but where routine surveillance is not undertaken and little incidence and prevalence data is available...
March 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29470509/co-circulation-of-all-the-four-dengue-virus-serotypes-and-detection-of-a-novel-clade-of-denv-4-genotype-i-virus-in-pune-india-during-2016-season
#12
Shubham Shrivastava, Divya Tiraki, Arundhati Diwan, Sanjay K Lalwani, Meera Modak, Akhilesh Chandra Mishra, Vidya A Arankalle
Dengue is the most common mosquito-borne viral infection in tropical and sub-tropical countries. In recent years, India has reported increased incidences of concurrent infection with multiple serotypes of dengue viruses (DENV). In the present study, we have characterized DENV circulating during a single season of 2016 in Pune, India. A total of 64 serum samples from NS1 ELISA positive dengue patients were used for PCR amplification of CprM region of the viral genome and sequencing. Phylogenetic analysis documented circulation of all the four DENV serotypes with predominance of DENV-2 (40...
2018: PloS One
https://www.readbyqxmd.com/read/29235427/dengue-hospitalisations-in-brazil-annual-wave-from-west-to-east-and-recent-increase-among-children
#13
J Wunderlich, R Acuña-Soto, W J Alonso
The number of dengue epidemics in Brazil has increased dramatically in the last 15 years. In this study, we analysed the seasonal patterns in the incidence of hospitalisations due to dengue across the different states of Brazil and compared these with the corresponding climatic patterns. We discovered that the seasonality of dengue hospitalisations in Brazil has a clear zonal gradient, characterised by the progression of primary peaks from West to East during the first half of the year, which may be associated with the increased vapour pressure and rainfall during this period, leading to increased mosquito abundance and activity...
January 2018: Epidemiology and Infection
https://www.readbyqxmd.com/read/29203103/association-of-tnfa-308g-a-ifng-874-a-t-and-il-10-819-c-t-polymorphisms-with-protection-and-susceptibility-to-dengue-in-brazilian-population
#14
Ana Caroline Melo Dos Santos, Edilson Leite de Moura, Jean Moises Ferreira, Alexandre Wendell Araujo de Moura, Ailson Darlan Sales Ferreira, Rubens Pereira Bezerra, Diego de Siqueira Figueiredo, Karol Fireman de Farias, Tiago Gomes de Andrade, Elaine Virgínia Martins de Souza Figueiredo
OBJECTIVE: To evaluate gene polymorphisms and their association with susceptibility to dengue. METHODS: A retrospective case-control study was performed with 262 subjects, comprising 78 dengue fever (DF) patients, 49 dengue hemorrhagic fever (DHF) patients and 135 healthy controls. Genotypic and allelic profiles were identified using polymerase chain reaction based in real time and amplification-refractory mutation system. RESULTS: We observed a protective association of IL-10 (-819 C/T) C allele (P = 0...
November 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29172075/real-time-rt-pcr-for-mayaro-virus-detection-in-plasma-and-urine
#15
Jesse J Waggoner, Alejandra Rojas, Alisha Mohamed-Hadley, Yvalena Arévalo de Guillén, Benjamin A Pinsky
BACKGROUND: Mayaro virus (MAYV) causes an acute febrile illness which can be difficult to differentiate from dengue or chikungunya. MAYV RNA can be detected in plasma during the first 3-5days of illness, but only a single rRT-PCR has been fully evaluated in the literature. OBJECTIVES: To develop an rRT-PCR for MAYV and evaluate assay performance using human plasma and urine samples spiked with different MAYV strains. STUDY DESIGN: A MAYV rRT-PCR targeting a region of the 5'UTR and nsp1 gene was designed from the alignment of all complete-genome MAYV sequences to be compatible with existing laboratory protocols...
January 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29161485/aedes-albopictus-skuse-1894-infected-with-the-american-asian-genotype-of-dengue-type-2-virus-in-medell%C3%A3-n-suggests-its-possible-role-as-vector-of-dengue-fever-in-colombia
#16
Andrés Gómez-Palacio, Juan Suaza-Vasco, Sandra Castaño, Omar Triana, Sandra Uribe
INTRODUCTION: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellín. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known. OBJECTIVE: To identify the presence of Ae...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29063884/molecular-and-phylogenetic-evidence-of-chikungunya-virus-circulating-in-assam-india
#17
Prafulla Dutta, Siraj Ahmed Khan, Naba Kumar Hazarika, Sumi Chetry
PURPOSE: Northeast Region of India possesses an abundant number of Aedes mosquitoes, the common vector for Dengue and Chikungunya (CHIK). Dengue is reported every year from Assam, but active surveillance for CHIK virus (CHIKV) infection is lacking in this part of India. Therefore, this present study has been undertaken to detect any CHIKV infection during a dengue outbreak in Assam. MATERIALS AND METHODS: A total of 42 dengue negative samples collected from Guwahati were screened for the presence of CHIK IgM antibodies...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29056517/molecular-characterization-of-dengue-viruses-isolated-from-patients-in-central-java-indonesia
#18
Endang S Kusmintarsih, Rahma F Hayati, Oktaviani N Turnip, Benediktus Yohan, Suhestri Suryaningsih, Hery Pratiknyo, Dionisius Denis, R Tedjo Sasmono
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto...
October 19, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28986247/the-reintroduction-of-denv-2-in-2011-in-panama-and-subsequent-outbreak-characteristic
#19
Yamilka Díaz, Julio Cisneros, Hilda Guzmán, Paola Cordoba, Jean-Paul Carrera, Brechla Moreno, Rubing Chen, Juan Castillo Mewa, Lourdes García, Lizbeth Cerezo, Amelia Travassos da Rosa, Nathan D Gundacker, Blas Armién, Scott C Weaver, Nikos Vasilakis, Sandra López-Vergès, Robert Tesh
The circulation of the South-east Asian/American (AS/AM) dengue 2 virus (DENV-2) genotype in the Americas has been associated with a high rate of severe disease. From 1993, the year DENV was reintroduced in Panama, until 2011 there were 29 dengue-associated deaths, 17 of which occurred in 2011, the most severe outbreak with a case fatality rate (CFR) of 44% (17 deaths out of 38 severe dengue cases). During this outbreak DENV-2 was reintroduced into the country, whereas over the prior five years DENV-1 and -3 were predominant...
October 3, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28978730/infection-rate-of-aedes-aegypti-mosquitoes-with-dengue-virus-depends-on-the-interaction-between-temperature-and-mosquito-genotype
#20
A Gloria-Soria, P M Armstrong, J R Powell, P E Turner
Dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. Temperature is epidemiologically relevant because it affects dengue infection rates in Aedes aegypti mosquitoes, the major vector of the dengue virus (DENV). Aedes aegypti populations are also known to vary in competence for different DENV genotypes. We assessed the effects of mosquito and virus genotype on DENV infection in the context of temperature by challenging Ae...
October 11, 2017: Proceedings. Biological Sciences
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