keyword
MENU ▼
Read by QxMD icon Read
search

genotyping dengue 2

keyword
https://www.readbyqxmd.com/read/29063884/molecular-and-phylogenetic-evidence-of-chikungunya-virus-circulating-in-assam-india
#1
Prafulla Dutta, Siraj Ahmed Khan, Naba Kumar Hazarika, Sumi Chetry
PURPOSE: Northeast Region of India possesses an abundant number of Aedes mosquitoes, the common vector for Dengue and Chikungunya (CHIK). Dengue is reported every year from Assam, but active surveillance for CHIK virus (CHIKV) infection is lacking in this part of India. Therefore, this present study has been undertaken to detect any CHIKV infection during a dengue outbreak in Assam. MATERIALS AND METHODS: A total of 42 dengue negative samples collected from Guwahati were screened for the presence of CHIK IgM antibodies...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29056517/molecular-characterization-of-dengue-viruses-isolated-from-patients-in-central-java-indonesia
#2
Endang S Kusmintarsih, Rahma F Hayati, Oktaviani N Turnip, Benediktus Yohan, Suhestri Suryaningsih, Hery Pratiknyo, Dionisius Denis, R Tedjo Sasmono
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto...
October 19, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28986247/the-reintroduction-of-denv-2-in-2011-in-panama-and-subsequent-outbreak-characteristic
#3
Yamilka Díaz, Julio Cisneros, Hilda Guzmán, Paola Cordoba, Jean-Paul Carrera, Brechla Moreno, Rubing Chen, Juan Castillo Mewa, Lourdes García, Lizbeth Cerezo, Amelia Travassos da Rosa, Nathan D Gundacker, Blas Armién, Scott C Weaver, Nikos Vasilakis, Sandra López-Vergès, Robert Tesh
The circulation of the South-east Asian/American (AS/AM) dengue 2 virus (DENV-2) genotype in the Americas has been associated with a high rate of severe disease. From 1993, the year DENV was reintroduced in Panama, until 2011 there were 29 dengue-associated deaths, 17 of which occurred in 2011, the most severe outbreak with a case fatality rate (CFR) of 44% (17 deaths out of 38 severe dengue cases). During this outbreak DENV-2 was reintroduced into the country, whereas over the prior five years DENV-1 and -3 were predominant...
October 3, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28978730/infection-rate-of-aedes-aegypti-mosquitoes-with-dengue-virus-depends-on-the-interaction-between-temperature-and-mosquito-genotype
#4
A Gloria-Soria, P M Armstrong, J R Powell, P E Turner
Dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. Temperature is epidemiologically relevant because it affects dengue infection rates in Aedes aegypti mosquitoes, the major vector of the dengue virus (DENV). Aedes aegypti populations are also known to vary in competence for different DENV genotypes. We assessed the effects of mosquito and virus genotype on DENV infection in the context of temperature by challenging Ae...
October 11, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28960920/a-proteo-genomic-analysis-of-haptoglobin-in-malaria
#5
Gauri Awasthi, Suchi Tyagi, Vipin Kumar, Sandip Kumar Patel, Dharmendar Rojh, Vijeth Sakrappanavar, Sanjay Kumar Kochar, Arunansu Talukdar, Biaus Samanta, Aparup Das, Sanjeeva Srivastava, Swati Patankar
Haptoglobin (Hp), an acute phase inflammatory protein has been associated with malaria pathogenesis in several proteomics studies. The gene has two co-dominant alleles: Hp1 and Hp2 that produce three genotypes: Hp1/Hp1, Hp1/Hp2 and Hp2/Hp2. In this study, we perform validation of the proteomics data with Multiple Reaction Monitoring Mass Spectroscopy (MRM-MS). We further analyze the association of the Hp gene variants with severe, non-severe malaria and community (healthy) controls using genotyping PCRs and DNA sequencing...
September 27, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28934991/the-dengue-preface-to-endemic-in-mainland-china-the-historical-largest-outbreak-by-aedes-albopictus-in-guangzhou-2014
#6
Lei Luo, Li-Yun Jiang, Xin-Cai Xiao, Biao Di, Qin-Long Jing, Sheng-Yong Wang, Jin-Ling Tang, Ming Wang, Xiao-Ping Tang, Zhi-Cong Yang
BACKGROUND: Dengue was regarded as a mild epidemic in mainland China transmitted by Aedes albopictus. However, the 2014 record-breaking outbreak in Guangzhou could change the situation. In order to provide an early warning of epidemic trends and provide evidence for prevention and control strategies, we seek to characterize the 2014 outbreak through application of detailed cases and entomological data, as well as phylogenetic analysis of viral envelope (E) gene. METHODS: We used case survey data identified through the Notifiable Infectious Disease Report System, entomological surveillance and population serosurvey, along with laboratory testing for IgM/IgG, NS1, and isolation of viral samples followed by E gene sequencing and phylogenetic analysis to examine the epidemiological and molecular characteristics of the outbreak...
September 22, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28930012/dengue-virus-1-outbreak-in-buenos-aires-argentina-2016
#7
Estefanía Tittarelli, Silvina B Lusso, Stephanie Goya, Gabriel L Rojo, Mónica I Natale, Mariana Viegas, Alicia S Mistchenko, Laura E Valinotto
The largest outbreak of dengue in Buenos Aires, Argentina, occurred during 2016. Phylogenetic, phylodynamic, and phylogeographic analyses of 82 samples from dengue patients revealed co-circulation of 2 genotype V dengue virus lineages, suggesting that this virus has become endemic to the Buenos Aires metropolitan area.
October 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28890510/circulating-genotypes-of-dengue-1-virus-in-south-west-india-2014-2015
#8
Shetty Pooja, Sasidharanpillai Sabeena, Bhaskar Revti, Ramachandran Sanjay, Aithal Anjali, Kumar Rajendra, Sushama Aswathyraj, Dsouza Giselle, Maity Hindol, Govindakarnavar Arunkumar
Dengue is found to be the most prevalent arboviral disease affecting humans. Tropical and subtropical regions are at high risk of dengue virus infections. The clinical manifestations often range from mild fever to fever with hemorrhagic manifestations and shock. Since 2010, India is witnessing a marked increase in the number of Dengue Virus Serotype 1(DENV-1) apart from Dengue Virus 2 (DENV-2) and Dengue Virus 3 (DENV-3) Serotypes. The present study was undertaken to understand the circulating genotypes of DENV-1 in South West India by sequencing the envelope gene of DENV-1 samples representative of the time period 2014-2015...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28796789/multiple-introductions-of-the-dengue-vector-aedes-aegypti-into-california
#9
Evlyn Pless, Andrea Gloria-Soria, Benjamin R Evans, Vicki Kramer, Bethany G Bolling, Walter J Tabachnick, Jeffrey R Powell
The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA) in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a) examining additional Northern CA samples and including samples from Southern CA, (b) including more southern US populations for comparison, and (c) genotyping a subset of samples at 15,698 SNPs...
August 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28764923/association-of-rs1285933-single-nucleotide-polymorphism-in-clec5a-gene-with-dengue-severity-and-its-functional-effects
#10
Caroline Xavier-Carvalho, Renata Duarte da Silva Cezar, Naishe Matos Freire, Carla Maria Mola de Vasconcelos, Victor Edgar Fiestas Solorzano, Thiago Gomes de Toledo-Pinto, Luciana Gomes Fialho, Rodrigo Feliciano do Carmo, Luydson Richardson Silva Vasconcelos, Marli Tenório Cordeiro, Paulo Baptista, Elzinandes Leal de Azeredo, Rivaldo Venâncio da Cunha, Luiz José de Souza, Antonio Guilherme Pacheco, Claire Fernandes Kubelka, Patrícia Muniz Mendes Freire de Moura, Milton Ozorio Moraes
Outbreaks of the Zika, dengue, and chikungunya viruses, especially in the Americas, pose a global threat due to their rapid spread and difficulty controlling the vector. Extreme phenotypes are often observed, from asymptomatic to severe clinical manifestations, which are well-studied in dengue. Host variations are also important contributors to disease outcomes, and many case-control studies have associated single nucleotide polymorphisms (SNPs) with severe dengue. Here, we found that the TC genotype and T-carriers for SNP rs1285933 in the C-type lectin superfamily member 5 (CLEC5A) gene was associated with severe dengue in a Northern Brazilian population (OR=2...
October 2017: Human Immunology
https://www.readbyqxmd.com/read/28727779/contemporary-status-of-insecticide-resistance-in-the-major-aedes-vectors-of-arboviruses-infecting-humans
#11
REVIEW
Catherine L Moyes, John Vontas, Ademir J Martins, Lee Ching Ng, Sin Ying Koou, Isabelle Dusfour, Kamaraju Raghavendra, João Pinto, Vincent Corbel, Jean-Philippe David, David Weetman
Both Aedes aegytpi and Ae. albopictus are major vectors of 5 important arboviruses (namely chikungunya virus, dengue virus, Rift Valley fever virus, yellow fever virus, and Zika virus), making these mosquitoes an important factor in the worldwide burden of infectious disease. Vector control using insecticides coupled with larval source reduction is critical to control the transmission of these viruses to humans but is threatened by the emergence of insecticide resistance. Here, we review the available evidence for the geographical distribution of insecticide resistance in these 2 major vectors worldwide and map the data collated for the 4 main classes of neurotoxic insecticide (carbamates, organochlorines, organophosphates, and pyrethroids)...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28725041/a-screen-for-novel-hepatitis-c-virus-rdrp-inhibitor-identifies-a-broad-spectrum-antiviral-compound
#12
Abhilasha Madhvi, Smita Hingane, Rajpal Srivastav, Nishant Joshi, Chandru Subramani, Rajagopalan Muthumohan, Renu Khasa, Shweta Varshney, Manjula Kalia, Sudhanshu Vrati, Milan Surjit, C T Ranjith-Kumar
Hepatitis C virus (HCV) is a global pathogen and infects more than 185 million individuals worldwide. Although recent development of direct acting antivirals (DAA) has shown promise in HCV therapy, there is an urgent need for the development of more affordable treatment options. We initiated this study to identify novel inhibitors of HCV through screening of compounds from the National Cancer Institute (NCI) diversity dataset. Using cell-based assays, we identified NSC-320218 as a potent inhibitor against HCV with an EC50 of 2...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719296/analysis-of-dengue-serotype-4-in-sri-lanka-during-the-2012-2013-dengue-epidemic
#13
Anna Uehara, Hasitha Aravinda Tissera, Champica K Bodinayake, Ananda Amarasinghe, Ajith Nagahawatte, L Gayani Tillekeratne, Jie Cui, Megan E Reller, Paba Palihawadana, Sunethra Gunasena, Aruna Dharshan Desilva, Annelies Wilder-Smith, Duane J Gubler, Christopher W Woods, October M Sessions
The four serotypes of dengue virus (DENV-1, -2, -3, and -4) have had a rapidly expanding geographic range and are now endemic in over 100 tropical and subtropical countries. Sri Lanka has experienced periodic dengue outbreaks since the 1960s, but since 1989 epidemics have become progressively larger and associated with more severe disease. The dominant virus in the 2012 epidemic was DENV-1, but DENV-4 infections were also commonly observed. DENV-4 transmission was first documented in Sri Lanka when it was isolated from a traveler in 1978, but has been comparatively uncommon since dengue surveillance began in the early 1980s...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28698156/disruption-of-predicted-dengue-virus-type-3-major-outbreak-cycle-coincided-with-switching-of-the-dominant-circulating-virus-genotype
#14
Kim-Kee Tan, Nurul-Izzani Zulkifle, Juraina Abd-Jamil, Syuhaida Sulaiman, Che Norainon Yaacob, Noor Syahida Azizan, Nurul Asma Anati Che Mat Seri, Nur Izyan Samsudin, Nur Hidayana Mahfodz, Sazaly AbuBakar
Dengue is hyperendemic in most of Southeast Asia. In this region, all four dengue virus serotypes are persistently present. Major dengue outbreak cycle occurs in a cyclical pattern involving the different dengue virus serotypes. In Malaysia, since the 1980s, the major outbreak cycles have involved dengue virus type 3 (DENV3), dengue virus type 1 (DENV1) and dengue virus type 2 (DENV2), occurring in that order (DENV3/DENV1/DENV2). Only limited information on the DENV3 cycles, however, have been described. In the current study, we examined the major outbreak cycle involving DENV3 using data from 1985 to 2016...
July 8, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28681719/an-outbreak-of-dengue-virus-denv-type-2-cosmopolitan-genotype-in-israeli-travellers-returning-from-the-seychelles-april-2017
#15
Yaniv Lustig, Dana Wolf, Ora Halutz, Eli Schwartz
Dengue virus infection was diagnosed in six Israeli travellers returning from the Seychelles in April 2017. Phylogenetic analysis identified identical sequences belonging to the Cosmopolitan genotype of dengue virus type 2 in all samples sequenced, thus providing evidence for a probable dengue type 2 outbreak in the Seychelles. This report further demonstrates the role of travellers as sentinels for arboviral infections, especially in countries with limited diagnostic capabilities.
June 29, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28674315/genomic-characterization-of-travel-associated-dengue-viruses-isolated-from-the-entry-exit-ports-in-fujian-province-china-2013-2015
#16
Bo Gao, Jianming Zhang, Yuping Wang, Fan Chen, Chaohui Zheng, Lianhui Xie
Over the past decade, indigenous dengue outbreaks have occurred occasionally in Fujian province in southeastern China because of sporadic imported dengue viruses (DENV). In this study, 3 DENV-2 and 2 DENV-4 strains were isolated from suspected febrile travelers at 2 ports of entry in Fujian between 2013-2015. Complete viral genome sequences of these new isolates were obtained with Sanger chemistry. Genomic sequence analyses revealed that these strains belonged to genotypes of 2-Cosmopolitan and 4-II. Consistent with the patients' travel information, phylogenetic analyses of the complete coding regions also indicated that most of the new isolates were genetically similar to the circulating strains in Southeast Asia rather than previous Chinese strains that were available...
September 25, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28648850/complete-genome-analysis-of-dengue-virus-type-3-isolated-from-the-2013-dengue-outbreak-in-yunnan-china
#17
Xiaodan Wang, Dehong Ma, Xinwei Huang, Lihua Li, Duo Li, Yujiao Zhao, Lijuan Qiu, Yue Pan, Junying Chen, Juemin Xi, Xiyun Shan, Qiangming Sun
In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent related to this largest outbreak of dengue fever. The viruses were isolated by cell culture and evaluated by genome sequence analysis. Phylogenetic trees were then constructed by Neighbor-Joining methods (MEGA6...
June 23, 2017: Virus Research
https://www.readbyqxmd.com/read/28648790/larval-stress-alters-dengue-virus-susceptibility-in-aedes-aegypti-l-adult-females
#18
David S Kang, Yehonatan Alcalay, Diane D Lovin, Joanne M Cunningham, Matthew W Eng, Dave D Chadee, David W Severson
In addition to genetic history, environmental conditions during larval stages are critical to the development, success and phenotypic fate of the Aedes aegypti mosquito. In particular, previous studies have shown a strong genotype-by-environment component to adult mosquito body size in response to optimal vs stressed larval conditions. Here, we expand upon those results by investigating the effects of larval-stage crowding and nutritional limitation on the susceptibility of a recent field isolate of Aedes aegypti to dengue virus serotype-2...
October 2017: Acta Tropica
https://www.readbyqxmd.com/read/28600724/phylogenetic-and-evolutionary-analyses-of-dengue-viruses-isolated-in-jakarta-indonesia
#19
C S Whinie Lestari, Benediktus Yohan, Anisa Yunita, Febrina Meutiawati, Rahma Fitri Hayati, Hidayat Trimarsanto, R Tedjo Sasmono
Dengue has affected Indonesia for the last five decades and become a major health problem in many cities in the country. Jakarta, the capital of Indonesia, reports dengue cases annually, with several outbreaks documented. To gain information on the dynamic and evolutionary history of dengue virus (DENV) in Jakarta, we conducted phylogenetic and evolutionary analyses of DENV isolated in 2009. Three hundred thirty-three dengue-suspected patients were recruited. Our data revealed that dengue predominantly affected young adults, and the majority of cases were due to secondary infection...
June 9, 2017: Virus Genes
https://www.readbyqxmd.com/read/28599625/genetic-variants-of-micb-and-plce1-and-associations-with-the-laboratory-features-of-dengue
#20
James Whitehorn, Duong Thi Hue Kien, Nguyen Than Ha Quyen, Bridget Wills, Nguyen Van Vinh Chau, Dong Thi Hoai Tam, Nguyen Minh Tuan, Thomas Jaenisch, Martin Hibberd, Chiea Chuen Khor, Cameron P Simmons
BACKGROUND: A previous genome-wide association study identified 2 susceptibility loci for severe dengue at MICB rs3132468 and PLCE1 rs3740360 and further work showed these mutations to be also associated with less severe clinical presentations. The aim of this study was to determine if these specific loci were associated with laboratory features of dengue that correlate with clinical severity with the aim of elucidating the functional basis of these genetic variants. METHODS: This was a case-only analysis of laboratory-confirmed dengue patients obtained from 2 prospective cohort studies and 1 randomised clinical trial in Vietnam (Trial registration: ISRCTN ISRCTN03147572...
June 9, 2017: BMC Infectious Diseases
keyword
keyword
66629
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"