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genotyping dengue 2

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https://www.readbyqxmd.com/read/28727779/contemporary-status-of-insecticide-resistance-in-the-major-aedes-vectors-of-arboviruses-infecting-humans
#1
REVIEW
Catherine L Moyes, John Vontas, Ademir J Martins, Lee Ching Ng, Sin Ying Koou, Isabelle Dusfour, Kamaraju Raghavendra, João Pinto, Vincent Corbel, Jean-Philippe David, David Weetman
Both Aedes aegytpi and Ae. albopictus are major vectors of 5 important arboviruses (namely chikungunya virus, dengue virus, Rift Valley fever virus, yellow fever virus, and Zika virus), making these mosquitoes an important factor in the worldwide burden of infectious disease. Vector control using insecticides coupled with larval source reduction is critical to control the transmission of these viruses to humans but is threatened by the emergence of insecticide resistance. Here, we review the available evidence for the geographical distribution of insecticide resistance in these 2 major vectors worldwide and map the data collated for the 4 main classes of neurotoxic insecticide (carbamates, organochlorines, organophosphates, and pyrethroids)...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28725041/a-screen-for-novel-hepatitis-c-virus-rdrp-inhibitor-identifies-a-broad-spectrum-antiviral-compound
#2
Abhilasha Madhvi, Smita Hingane, Rajpal Srivastav, Nishant Joshi, Chandru Subramani, Rajagopalan Muthumohan, Renu Khasa, Shweta Varshney, Manjula Kalia, Sudhanshu Vrati, Milan Surjit, C T Ranjith-Kumar
Hepatitis C virus (HCV) is a global pathogen and infects more than 185 million individuals worldwide. Although recent development of direct acting antivirals (DAA) has shown promise in HCV therapy, there is an urgent need for the development of more affordable treatment options. We initiated this study to identify novel inhibitors of HCV through screening of compounds from the National Cancer Institute (NCI) diversity dataset. Using cell-based assays, we identified NSC-320218 as a potent inhibitor against HCV with an EC50 of 2...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719296/analysis-of-dengue-serotype-4-in-sri-lanka-during-the-2012-2013-dengue-epidemic
#3
Anna Uehara, Hasitha Aravinda Tissera, Champica K Bodinayake, Ananda Amarasinghe, Ajith Nagahawatte, L Gayani Tillekeratne, Jie Cui, Megan E Reller, Paba Palihawadana, Sunethra Gunasena, Aruna Dharshan Desilva, Annelies Wilder-Smith, Duane J Gubler, Christopher W Woods, October M Sessions
The four serotypes of dengue virus (DENV-1, -2, -3, and -4) have had a rapidly expanding geographic range and are now endemic in over 100 tropical and subtropical countries. Sri Lanka has experienced periodic dengue outbreaks since the 1960s, but since 1989 epidemics have become progressively larger and associated with more severe disease. The dominant virus in the 2012 epidemic was DENV-1, but DENV-4 infections were also commonly observed. DENV-4 transmission was first documented in Sri Lanka when it was isolated from a traveler in 1978, but has been comparatively uncommon since dengue surveillance began in the early 1980s...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28698156/disruption-of-predicted-dengue-virus-type-3-major-outbreak-cycle-coincided-with-switching-of-the-dominant-circulating-virus-genotype
#4
Kim-Kee Tan, Nurul-Izzani Zukifle, Juraina Abd-Jamil, Syuhaida Sulaiman, Che Norainon Yaacob, Noor Syahida Azizan, Nurul Asma Anati Che Mat Seri, Nur Izyan Samsudin, Nur Hidayana Mahfodz, Sazaly AbuBakar
Dengue is hyperendemic in most of Southeast Asia. In this region, all four dengue virus serotypes are persistently present. Major dengue outbreak cycle occurs in a cyclical pattern involving the different dengue virus serotypes. In Malaysia, since the 1980s, the major outbreak cycles have involved dengue virus type 3 (DENV3), dengue virus type 1 (DENV1) and dengue virus type 2 (DENV2), occurring in that order (DENV3/DENV1/DENV2). Only limited information on the DENV3 cycles, however, have been described. In the current study, we examined the major outbreak cycle involving DENV3 using data from 1981 to 2016...
July 8, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28681719/an-outbreak-of-dengue-virus-denv-type-2-cosmopolitan-genotype-in-israeli-travellers-returning-from-the-seychelles-april-2017
#5
Yaniv Lustig, Dana Wolf, Ora Halutz, Eli Schwartz
Dengue virus infection was diagnosed in six Israeli travellers returning from the Seychelles in April 2017. Phylogenetic analysis identified identical sequences belonging to the Cosmopolitan genotype of dengue virus type 2 in all samples sequenced, thus providing evidence for a probable dengue type 2 outbreak in the Seychelles. This report further demonstrates the role of travellers as sentinels for arboviral infections, especially in countries with limited diagnostic capabilities.
June 29, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28674315/genomic-characterization-of-travel-associated-dengue-viruses-isolated-at-entry-exit-ports-in-fujian-province-china-2013-2015
#6
Bo Gao, Jianming Zhang, Yuping Wang, Fan Chen, Chaohui Zheng, Lianhui Xie
During the past decade, indigenous dengue outbreaks occurred occasionally in Fujian province, southeast China, due to sporadic imported dengue viruses (DENV). In this study, a total of three DENV-2 and two DENV-4 strains were isolated from suspected febrile travelers at two ports-of-entry in Fujian during 2013-2015. Complete viral genome sequences of these new isolates were obtained with Sanger chemistry. Genomic sequence analysis revealed that these strains belonged to genotypes of 2-Cosmopolitan and 4-II...
July 1, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28648850/complete-genome-analysis-of-dengue-virus-type-3-isolated-from-the-2013-dengue-outbreak-in-yunnan-china
#7
Xiaodan Wang, Dehong Ma, Xinwei Huang, Lihua Li, Duo Li, Yujiao Zhao, Lijuan Qiu, Yue Pan, Junying Chen, Juemin Xi, Xiyun Shan, Qiangming Sun
In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent related to this largest outbreak of dengue fever. The viruses were isolated by cell culture and evaluated by genome sequence analysis. Phylogenetic trees were then constructed by Neighbor-Joining methods (MEGA6...
June 23, 2017: Virus Research
https://www.readbyqxmd.com/read/28648790/larval-stress-alters-dengue-virus-susceptibility-in-aedes-aegypti-l-adult-females
#8
David S Kang, Yehonatan Alcalay, Diane D Lovin, Joanne M Cunningham, Matthew W Eng, Dave D Chadee, David W Severson
In addition to genetic history, environmental conditions during larval stages are critical to the development, success and phenotypic fate of the Aedes aegypti mosquito. In particular, previous studies have shown a strong genotype-by-environment component to adult mosquito body size in response to optimal vs stressed larval conditions. Here, we expand upon those results by investigating the effects of larval-stage crowding and nutritional limitation on the susceptibility of a recent field isolate of Aedes aegypti to dengue virus serotype-2...
June 23, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28600724/phylogenetic-and-evolutionary-analyses-of-dengue-viruses-isolated-in-jakarta-indonesia
#9
C S Whinie Lestari, Benediktus Yohan, Anisa Yunita, Febrina Meutiawati, Rahma Fitri Hayati, Hidayat Trimarsanto, R Tedjo Sasmono
Dengue has affected Indonesia for the last five decades and become a major health problem in many cities in the country. Jakarta, the capital of Indonesia, reports dengue cases annually, with several outbreaks documented. To gain information on the dynamic and evolutionary history of dengue virus (DENV) in Jakarta, we conducted phylogenetic and evolutionary analyses of DENV isolated in 2009. Three hundred thirty-three dengue-suspected patients were recruited. Our data revealed that dengue predominantly affected young adults, and the majority of cases were due to secondary infection...
June 9, 2017: Virus Genes
https://www.readbyqxmd.com/read/28599625/genetic-variants-of-micb-and-plce1-and-associations-with-the-laboratory-features-of-dengue
#10
James Whitehorn, Duong Thi Hue Kien, Nguyen Than Ha Quyen, Bridget Wills, Nguyen Van Vinh Chau, Dong Thi Hoai Tam, Nguyen Minh Tuan, Thomas Jaenisch, Martin Hibberd, Chiea Chuen Khor, Cameron P Simmons
BACKGROUND: A previous genome-wide association study identified 2 susceptibility loci for severe dengue at MICB rs3132468 and PLCE1 rs3740360 and further work showed these mutations to be also associated with less severe clinical presentations. The aim of this study was to determine if these specific loci were associated with laboratory features of dengue that correlate with clinical severity with the aim of elucidating the functional basis of these genetic variants. METHODS: This was a case-only analysis of laboratory-confirmed dengue patients obtained from 2 prospective cohort studies and 1 randomised clinical trial in Vietnam (Trial registration: ISRCTN ISRCTN03147572...
June 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28575000/clinical-and-virological-characteristics-of-dengue-in-surabaya-indonesia
#11
Puspa Wardhani, Aryati Aryati, Benediktus Yohan, Hidayat Trimarsanto, Tri Y Setianingsih, Dwiyanti Puspitasari, Muhammad Vitanata Arfijanto, Bramantono Bramantono, Suharto Suharto, R Tedjo Sasmono
Dengue disease is still a major health problem in Indonesia. Surabaya, the second largest city in the country, is endemic for dengue. We report here on dengue disease in Surabaya, investigating the clinical manifestations, the distribution of dengue virus (DENV) serotypes, and the relationships between clinical manifestations and the genetic characteristics of DENV. A total of 148 patients suspected of having dengue were recruited during February-August 2012. One hundred one (68%) of them were children, and 47 (32%) were adults...
2017: PloS One
https://www.readbyqxmd.com/read/28544099/genetic-signatures-coupled-with-lineage-shift-characterise-endemic-evolution-of-dengue-virus-serotype-2-during-2015-outbreak-in-delhi-india
#12
Manish Chandra Choudhary, Ekta Gupta, Shvetank Sharma, Nadeem Hasnain, Pragya Agarwala
OBJECTIVE: In 2015, New Delhi witnessed a massive outbreak of Dengue virus (DENV) resulting in high morbidity and mortality. We report the molecular characterisation of the dominant circulating DENV strain to understand its evolution and dispersal. MATERIALS AND METHODS: DENV infections were diagnosed by detection of IgM/NS1 antigen, and serotyping was performed by C-PrM PCR. Envelope gene was amplified, and variation(s) in envelope gene were analysed. Phylogenetic tree construction, time-based phylogeny and origin of DENV were analysed...
July 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28531223/dengue-in-bali-clinical-characteristics-and-genetic-diversity-of-circulating-dengue-viruses
#13
Dewi Megawati, Sri Masyeni, Benediktus Yohan, Asri Lestarini, Rahma F Hayati, Febrina Meutiawati, Ketut Suryana, Tangking Widarsa, Dewa G Budiyasa, Ngurah Budiyasa, Khin S A Myint, R Tedjo Sasmono
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 205 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and dengue screening was performed using NS1 and IgM/IgG ELISAs...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28482813/epidemiological-and-molecular-characteristics-of-emergent-dengue-virus-in-yunnan-province-near-the-china-myanmar-laos-border-2013-2015
#14
Ting-Song Hu, Hai-Lin Zhang, Yun Feng, Jian-Hua Fan, Tian Tang, Yong-Hua Liu, Liu Zhang, Xiao-Xiong Yin, Gang Chen, Hua-Chang Li, Jin Zu, Hong-Bin Li, Yuan-Yuan Li, Jing Yu, Fu-Qiang Zhang, Quan-Shui Fan
BACKGROUND: Yunnan Province is located in southwestern China and neighbors the Southeast Asian countries, all of which are dengue-endemic areas. In 2000-2013, sporadic imported cases of dengue fever (DF) were reported almost annually in Yunnan Province. During 2013-2015, we confirmed that a large-scale indigenous DF outbreak emerged in cities of Yunnan Province near the China-Myanmar-Laos border. METHODS: Epidemiological characteristics of DF in Yunnan Province during 2013-2015 were evaluated by retrospective analysis...
May 8, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28456663/comparative-whole-genome-analysis-of-dengue-virus-serotype-2-strains-differing-in-trans-endothelial-cell-leakage-induction-in-vitro
#15
Sneha Singh, M G Anupriya, Easwaran Sreekumar
The role of genetic differences among dengue virus (DENV) in causing increased microvascular permeability is less explored. In the present study, we compared two closely related DENV serotype-2 strains of Cosmopolitan genotype for their in vitro infectivity phenotype and ability to induce trans-endothelial leakage. We found that these laboratory strains differed significantly in infecting human microvascular endothelial cells (HMEC-1) and hepatocytes (Huh7), two major target cells of DENV in in vivo infections...
April 27, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28450505/complete-coding-sequences-of-two-dengue-virus-type-2-strains-isolated-from-an-outbreak-in-burkina-faso-in-2016
#16
Cécile Baronti, Géraldine Piorkowski, Franck Touret, Rémi Charrel, Xavier de Lamballerie, Antoine Nougairede
We report here the complete coding sequences of two strains of dengue virus type 2, isolated in France from patients returning from Burkina Faso in November 2016. Both strains (cosmopolitan genotype) are almost identical (99.91% nucleotide identity) and closely related to a strain circulating in Burkina Faso in 1983.
April 27, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28388922/viral-and-serological-kinetics-in-zika-virus-infected-patients-in-south-korea
#17
Young Eui Jeong, Go-Woon Cha, Jung Eun Cho, Eun Ju Lee, Youngmee Jee, Won-Ja Lee
Zika virus is a mosquito-borne flavivirus that causes clinical symptoms similar to those observed in dengue and chikungunya virus infections. The Korea Centers for Disease Control and Prevention initiated laboratory testing using a real-time reverse transcription-polymerase chain reaction in January 2016. More than 1,000 suspected cases of infection were tested and nine were confirmed as imported cases of Zika virus infection from January to July 2016. The travel destinations of the infected individuals were Brazil, Philippines, Viet Nam, Guatemala, Puerto Rico, and the Dominican Republic...
April 7, 2017: Virology Journal
https://www.readbyqxmd.com/read/28367875/laboratory-and-molecular-characterization-of-dengue-virus-during-the-2014-outbreak-in-guangfo-region-southern-china
#18
Zhao-Fan Luo, Bo Hu, Feng-Yi Zhang, Xiang-Hua Lin, Xiao-Ying Xie, Kun-Yi Pan, Hong-Yu Li, Rui-Wen Ren, Wen-Zhong Zhao
Non-specific symptoms and low viremia levels make early diagnosis of dengue virus (DENV) infection challenging. This study aimed to 1) identify laboratory markers which can predict a positive laboratory diagnosis, and 2) perform a molecular characterization of DENV from the 2014 Guangdong epidemic. This retrospective study enrolled 1044 patients from the Guangdong epidemic who had clinically suspected dengue. Viral RNA was detected by real-time RT-PCR and viral-specific NS1 antigen was detected using ELISA...
March 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28241052/osbpl10-rxra-and-lipid-metabolism-confer-african-ancestry-protection-against-dengue-haemorrhagic-fever-in-admixed-cubans
#19
Beatriz Sierra, Petr Triska, Pedro Soares, Gissel Garcia, Ana B Perez, Eglys Aguirre, Marisa Oliveira, Bruno Cavadas, Béatrice Regnault, Mayling Alvarez, Didye Ruiz, David C Samuels, Anavaj Sakuntabhai, Luisa Pereira, Maria G Guzman
Ethnic groups can display differential genetic susceptibility to infectious diseases. The arthropod-born viral dengue disease is one such disease, with empirical and limited genetic evidence showing that African ancestry may be protective against the haemorrhagic phenotype. Global ancestry analysis based on high-throughput genotyping in admixed populations can be used to test this hypothesis, while admixture mapping can map candidate protective genes. A Cuban dengue fever cohort was genotyped using a 2.5 million SNP chip...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28120220/molecular-epidemiology-demonstrates-that-imported-and-local-strains-circulated-during-the-2014-dengue-outbreak-in-guangzhou-china
#20
Geng Li, Pan Pan, Qiuyan He, Xiujuan Kong, Kailang Wu, Wei Zhang, Yuntao Liu, Huiting Huang, Jianbo Liu, Zhongde Zhang, De Wu, Xiaoping Lai, Xiaohong Liu, Jianguo Wu
The dengue virus (DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope (E) gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype I strains, including 8 genotype I and 9 genotype V strains...
February 2017: Virologica Sinica
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