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genotyping dengue 2

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https://www.readbyqxmd.com/read/27902331/the-plasma-virome-of-febrile-adult-kenyans-shows-frequent-parvovirus-b19-infections-and-a-novel-arbovirus-kadipiro-virus
#1
Carolyne Ngoi, Juliana Siqueira, Linlin Li, Xutao Deng, Peter Mugo, Susan Graham, Matt Price, Eduard Sanders, Eric Delwart
Viral nucleic acids present in the plasma of 498 Kenyan adults with unexplained fever were characterized by metagenomics analysis of 51 sample pools. The highest to lowest fraction of plasma pools was positive for parvovirus B19 (75%), pegivirus C (GBV-C) (67%), alpha anellovirus (59%), gamma anellovirus (55%), beta anellovirus (41%), Dengue virus genotype 2 (DENV-2) (16%), HIV-1 (6%), HHV-6 (6%), HBV (4%), rotavirus (4%), norovirus (4%), rhinovirus C (2%), Merkel cell polyomavirus (MCPyV)(2%) and Kadipiro virus (2%)...
October 24, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27765124/-study-of-epidemiological-characteristics-and-viral-sources-of-dengue-fever-outbreak-in-guangxi-zhuang-autonomous-region-2014
#2
M M Chen, Y Tan, Z Z Tang, M Lin, K J Zhou, W T He, Y P Yang, J Wang
Objective: To understand the epidemiological characteristics and viral sources of dengue fever outbreak in Guangxi Zhuang Autonomous Region (Guangxi) in 2014. Methods: A combined analysis of epidemiological characteristics and genetic characteristics were performed in this study. The time, population and area distributions of the cases were analyzed. Serum samples were collected from dengue fever cases to detect NS1 antigen by using commercial ELISA kits according to the guideline of the manufacture. RT-PCR assay was conducted to detect dengue virus in NS1 positive samples...
October 10, 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/27722053/epidemiological-and-molecular-characterization-of-dengue-viruses-imported-into-guangzhou-during-2009-2013
#3
Yongxia Shi, Shufen Li, Xiaobo Li, Kui Zheng, Shuai Yuan, Jicheng Huang
BACKGROUND: Dengue virus causes one of the most significant infectious diseases in tropical and subtropical regions, notable number of which is imported into China every year. RESULTS: In this study, the molecular epidemiologic and phylogenetic analyses of dengue cases imported into Guangzhou in South China during 2009-2013 were conducted. During that period, 46 imported dengue cases were identified, including four serotypes. Most of the dengue patients were travelling from Southeast Asia, South Asia and Africa...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27681163/molecular-characterization-and-viral-origin-of-the-2015-dengue-outbreak-in-xishuangbanna-yunnan-china
#4
Yujiao Zhao, Lihua Li, Dehong Ma, Jia Luo, Zhiqiang Ma, Xiaodan Wang, Yue Pan, Junying Chen, Juemin Xi, Jiajia Yang, Lijuan Qiu, Chunhai Bai, Liming Jiang, Xiyun Shan, Qiangming Sun
A total of 1067 serum samples were collected from febrile patients in Xishuangbanna, Yunnan, 2015. Of these, 852 cases were confirmed to be dengue NS1-positive. 76 structural protein genes were sequenced through RT-PCR based on the viral RNAs extracted from serum samples. Phylogenetic analysis revealed that all strains were classified as cosmopolitan genotype of DENV-2. After comparing with the DENV-2SS, 173 base substitutions were found in 76 sequences, resulting in 43 nonsynonymous mutations, of which 22 mutations existed among all samples...
September 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27635316/analysis-of-genotype-diversity-and-evolution-of-dengue-virus-serotype-2-using-complete-genomes
#5
Vaishali P Waman, Pandurang Kolekar, Mukund R Ramtirthkar, Mohan M Kale, Urmila Kulkarni-Kale
BACKGROUND: Dengue is one of the most common arboviral diseases prevalent worldwide and is caused by Dengue viruses (genus Flavivirus, family Flaviviridae). There are four serotypes of Dengue Virus (DENV-1 to DENV-4), each of which is further subdivided into distinct genotypes. DENV-2 is frequently associated with severe dengue infections and epidemics. DENV-2 consists of six genotypes such as Asian/American, Asian I, Asian II, Cosmopolitan, American and sylvatic. Comparative genomic study was carried out to infer population structure of DENV-2 and to analyze the role of evolutionary and spatiotemporal factors in emergence of diversifying lineages...
2016: PeerJ
https://www.readbyqxmd.com/read/27620341/occurrence-of-co-infection-with-dengue-viruses-during-2014-in-new-delhi-india
#6
A Tazeen, N Afreen, M Abdullah, F Deeba, S H Haider, S N Kazim, S Ali, I H Naqvi, S Broor, A Ahmed, S Parveen
Dengue fever is an arthropod-borne viral infection that has become endemic in several parts of India including Delhi. We studied occurrence of co-infection with dengue viruses during an outbreak in New Delhi, India in 2014. For the present study, blood samples collected from symptomatic patients were analysed by RT-PCR. Eighty percent of the samples were positive for dengue virus. The result showed that DENV-1 (77%) was the predominant serotype followed by DENV-2 (60%). Concurrent infection with more than one serotype was identified in 43% of the positive samples...
January 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/27609639/dengue-virus-surveillance-detection-of-denv-4-in-the-city-of-s%C3%A3-o-jos%C3%A3-do-rio-preto-sp-brazil
#7
Tatiana Elias Colombo, Danila Vedovello, Carolina Colombelli Pacca-Mazaro, Adriano Mondini, João Pessoa Araújo, Eliana Cabrera, José Carlos Lopes, Izalco Nuremberg Penha Dos Santos, Andréia Francesli Negri Reis, Fabiana Rodrigues Costa, Lilian Elisa Arão Antônio Cruz, Jurandir Ferreira, Eliseu Soares de Oliveira Rocha, Erna Geessien Kroon, Roberta Vieira de Morais Bronzoni, Nikos Vasilakis, Maurício Lacerda Nogueira
Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nested-PCR assay with species-specific primers...
September 5, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27563048/first-complete-genome-sequence-of-a-chikungunya-virus-strain-isolated-from-a-patient-diagnosed-with-dengue-virus-infection-in-malaysia
#8
Man Kwan Ooi, Han Ming Gan, Ahmad Rohani, Sharifah Syed Hassan
Here, we report the complete genome sequence of a chikungunya virus coinfection strain isolated from a dengue virus serotype 2-infected patient in Malaysia. This coinfection strain was determined to be of the Asian genotype and contains a novel insertion in the nsP3 gene.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27524929/co-circulation-of-the-dengue-with-chikungunya-virus-during-the-2013-outbreak-in-the-southern-part-of-lao-pdr
#9
Viengvaly Phommanivong, Seiji Kanda, Takaki Shimono, Pheophet Lamaningao, Andrew Waleluma Darcy, Nobuyuki Mishima, Bounthanh Phaytanavanh, Toshimasa Nishiyama
BACKGROUND: During the 2013 outbreak, 4638 infection cases and 32 deaths have been recorded in the southern part of Laos. In recent years, the chikungunya virus (CHIKV) emerged in the part of the country bordering Cambodia. Dengue virus (DENV) and CHIKV are transmitted by common mosquito vectors. Both diseases have similar clinical presentations; therefore, CHIKV infections might go undiagnosed in DENV-endemic areas. Thus, rapid detection and accurate diagnosis are crucial for differentiating between the two viruses (DENV and CHIKV)...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27436278/association-of-genetic-polymorphisms-of-il1%C3%AE-511-c-t-il1rn-vntr-86%C3%A2-bp-il6-174-g-c-il10-819-c-t-and-tnf%C3%AE-308-g-a-involved-in-symptomatic-patients-with-dengue-in-brazil
#10
Isaac Farias Cansanção, Ana Paula Santos do Carmo, Robério Dias Leite, Rosana Deyse Ponte Portela, Sérgio de Sá Leitão Paiva Júnior, Valdir de Queiroz Balbino, Silvia Helena Barem Rabenhorst
OBJECTIVE: In this study, single nucleotide polymorphisms (SNP) of interleukin (IL) 1β -511C>T, IL1RN VNTR 86 bp, IL6 -174G>C, IL10 -819C>T and TNFα -308G>A were analyzed by PCR-RFLP with symptoms of dengue with the clinical features. SUBJECTS: 196 individuals admitted to the São José Infectious Diseases Hospital with suspected dengue infection. Dengue was confirmed in 111 of the patients. The control group consisted of 85 other individuals confirmed without dengue...
November 2016: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/27401010/association-of-bak1-single-nucleotide-polymorphism-with-a-risk-for-dengue-hemorrhagic-fever
#11
Tran Ngoc Dang, Izumi Naka, Areerat Sa-Ngasang, Surapee Anantapreecha, Nuanjun Wichukchinda, Pathom Sawanpanyalert, Jintana Patarapotikul, Naoyuki Tsuchiya, Jun Ohashi
BACKGROUND: Dengue hemorrhagic fever (DHF) is a severe life-threatening form of dengue infection. Low platelet count is one of the characteristic clinical manifestations in patients with severe dengue. However, little is known about genetic factors in the host that cause low platelet count in patients with dengue. METHODS: A previous genome-wide association study of hematological and biochemical traits identified single nucleotide polymorphisms (SNPs) associated with low platelet count in healthy subjects...
2016: BMC Medical Genetics
https://www.readbyqxmd.com/read/27336361/gene-polymorphisms-and-serum-levels-of-pro-and-anti-inflammatory-markers-in-dengue-viral-infections
#12
Rosimar Neris Martins Feitosa, Antonio Carlos Rosário Vallinoto, Pedro Fernando da Costa Vasconcelos, Raimunda do Socorro da Silva Azevedo, Vânia Nakauth Azevedo, Luiz Fernando Almeida Machado, Sandra Souza Lima, Marluísa de Oliveira Guimarães Ishak, Ricardo Ishak
Pro- and anti-inflammatory markers (tumor necrosis factor [TNF]-α, TNF-β, interferon [IFN]-γ, interleukin [IL]-6, IL-8, IL-10, and C-reactive protein [CRP]) were investigated in 80 patients infected with dengue viruses, 100 patients presenting with febrile illness but negative for dengue, and 99 healthy subjects. Immunoenzyme methods were used for quantitative assays in the plasma. Polymorphisms of TNF-α, TNF-β, IL-6, IL-8, and IL-10 genes were assessed by polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific oligonucleotide (ASO)-PCR for the IFN-γ...
September 2016: Viral Immunology
https://www.readbyqxmd.com/read/27330849/epidemiology-of-chikungunya-virus-in-bahia-brazil-2014-2015
#13
Nuno Rodrigues Faria, José Lourenço, Erenilde Marques de Cerqueira, Maricélia Maia de Lima, Oliver Pybus, Luiz Carlos Junior Alcantara
Chikungunya is an emerging arbovirus that is characterized into four lineages. One of these, the Asian genotype, has spread rapidly in the Americas after its introduction in the Saint Martin island in October 2013. Unexpectedly, a new lineage, the East-Central-South African genotype, was introduced from Angola in the end of May 2014 in Feira de Santana (FSA), the second largest city in Bahia state, Brazil, where over 5,500 cases have now been reported. Number weekly cases of clinically confirmed CHIKV in FSA were analysed alongside with urban district of residence of CHIKV cases reported between June 2014 and October collected from the municipality's surveillance network...
2016: PLoS Currents
https://www.readbyqxmd.com/read/27316694/epidemic-resurgence-of-dengue-fever-in-singapore-in-2013-2014-a-virological-and-entomological-perspective
#14
Hapuarachchige Chanditha Hapuarachchi, Carmen Koo, Jayanthi Rajarethinam, Chee-Seng Chong, Cui Lin, Grace Yap, Lilac Liu, Yee-Ling Lai, Peng Lim Ooi, Jeffery Cutter, Lee-Ching Ng
BACKGROUND: Dengue resurged in Singapore during 2013-14, causing an outbreak with unprecedented number of cases in the country. In the present study, we summarise the epidemiological, virological and entomological findings gathered through the dengue surveillance programme and highlight the drivers of the epidemic. We also describe how the surveillance system facilitated the preparedness to moderate epidemic transmission of dengue in the country. METHODS: The case surveillance was based on a mandatory notification system that requires all medical practitioners to report clinically-suspected and laboratory-confirmed cases within 24 hours...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27302095/analysis-of-tlr4-asp299gly-and-thr399ile-gene-polymorphisms-and-mrna-level-in-patients-with-dengue-infection-a-case-control-study
#15
Swati Sharma, Satyendra K Singh, Kavita Kakkar, Nikky Nyari, Tapan N Dhole, Rajesh Kashyap, Saba Hasan
BACKGROUND: Dengue is a systemic viral infection that spreads to humans by the bite of infected Aedes mosquitoes. The secreted NS1 protein of dengue virus activates macrophages and human PBMCs via TLR4 and induce the release of pro-inflammatory cytokines which is responsible for the pathogenesis of disease. Mutations in TLR4 gene have been associated with the increased susceptibility to many viral, bacterial and parasitic diseases. OBJECTIVE: To study the impact of TLR4 Asp299Gly (rs4986790) and Thr399Ile (rs4986791) gene polymorphisms with susceptibility to dengue infection...
September 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27257804/epidemiological-and-virological-characterizations-of-the-2014-dengue-outbreak-in-guangzhou-china
#16
Hui Zhao, Fu-Chun Zhang, Qin Zhu, Jian Wang, Wen-Xin Hong, Ling-Zhai Zhao, Yong-Qiang Deng, Shuang Qiu, Yu Zhang, Wei-Ping Cai, Wu-Chun Cao, Cheng-Feng Qin
Dengue used to be recognized as an imported and sporadic disease in China. Since June 2014, an unexpected large dengue outbreak has attacked Guangzhou, China, resulting in more than 40,000 cases. Among the 1,942 laboratory-confirmed hospitalized dengue cases, 121 were diagnosed as severe dengue according to the 2009 WHO guideline, and 2 patients finally died. Laboratory diagnosis and virus isolation demonstrated that the majority (96%) cases were caused by dengue virus serotype 1 (DENV-1), and the others by serotype 2 (DENV-2)...
2016: PloS One
https://www.readbyqxmd.com/read/27215933/the-molecular-and-clinical-features-of-dengue-during-outbreak-in-jambi-indonesia-in-2015
#17
Sotianingsih Haryanto, Rahma F Hayati, Benediktus Yohan, Lanceria Sijabat, Ifo F Sihite, Sukmal Fahri, Febrina Meutiawati, Jonathan A N Halim, Stefanie N Halim, Amin Soebandrio, R Tedjo Sasmono
Dengue is hyperendemic in Indonesia. In 2015, reported cases of dengue fever doubled those of 2014 in the Jambi municipality of Sumatra. We examined viral aetiology and its relationship with disease outcome in Jambi. Dengue-suspected patients' sera were collected and NS1 detection and IgM/IgG serology were performed. Dengue virus (DENV) serotyping was performed using real-time RT-PCR. Envelope genes were sequenced to determine the genotypes of DENV. Clinical, haematologic, and demographic data were recorded...
May 2016: Pathogens and Global Health
https://www.readbyqxmd.com/read/27154331/characterization-of-the-2013-dengue-epidemic-in-myanmar-with-dengue-virus-1-as-the-dominant-serotype
#18
Mya Myat Ngwe Tun, Aung Kyaw Kyaw, Nader Makki, Rohitha Muthugala, Takeshi Nabeshima, Shingo Inoue, Daisuke Hayasaka, Meng Ling Moi, Corazon C Buerano, Saw Myat Thwe, Kyaw Zin Thant, Kouichi Morita
In 2013 in Myanmar, dengue epidemic occurred with 20,255 cases including 84 deaths. This study aimed to determine the serological and molecular characteristics of dengue virus (DENV) infection among children with clinical diagnosis of dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) during this period. Single acute serum samples were collected from 300 children in Mandalay Children Hospital, Mandalay, Myanmar. Out of the 300 children, 175 (58.3%) and 183 (61%) were positive for anti-dengue IgM and anti-dengue IgG, respectively...
September 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27139442/molecular-characterization-of-two-major-dengue-outbreaks-in-costa-rica
#19
Claudio Soto-Garita, Teresita Somogyi, Amanda Vicente-Santos, Eugenia Corrales-Aguilar
Dengue virus (DENV) (Flavivirus, Flaviviridae) is a reemerging arthropod-borne virus with a worldwide circulation, transmitted mainly by Aedes aegypti and Aedes albopictus mosquitoes. Since the first detection of its main transmitting vector in 1992 and the invasion of DENV-1 in 1993, Costa Rica has faced dengue outbreaks yearly. In 2007 and 2013, Costa Rica experienced two of the largest outbreaks in terms of total and severe cases. To provide genetic information about the etiologic agents producing these outbreaks, we conducted phylogenetic analysis of viruses isolated from human samples...
July 6, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27135750/multiple-introductions-of-dengue-2-virus-strains-into-saudi-arabia-from-1992-to-2014
#20
Sherif A El-Kafrawy, Sayed S Sohrab, Said Abol Ela, Adly M M Abd-Alla, Rowa Alhabbab, Suha A Farraj, Norah A Othman, Ahmed M Hassan, Max Bergoin, Raphaelle Klitting, Remi N Charrel, Anwar M Hashem, Tariq A Madani, Esam I Azhar
INTRODUCTION: Dengue is a significant arboviral infection that represents a major public health concern worldwide. The infection is endemic in most parts of South East Asia, sub-Saharan Africa, and Latin America. Among the four dengue virus (DENV) serotypes, DENV-2 has been reported to be the predominant serotype in Saudi Arabia since 1992. However, virological and epidemiological data of DENV-2 from Saudi Arabia are severely deficient and require further investigations. METHODS: Full genome sequencing of a recent DENV-2 isolate and phylogenetic analysis of all available DENV-2 sequences from Saudi Arabia...
June 2016: Vector Borne and Zoonotic Diseases
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