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https://www.readbyqxmd.com/read/29346641/uncovering-behavioural-diversity-amongst-high-strength-pseudomonas-spp-surfactants-at-the-limit-of-liquid-surface-tension-reduction
#1
Kamaluddeen Kabir, Yusuf Y Deeni, Simona M Hapca, Luke Moore, Andrew J Spiers
Bacterial bio-surfactants have a wide range of biological functions and biotechnological applications. Previous analyses had suggested a limit to their reduction of aqueous liquid surface tensions (γMin), and here we confirm this in an analysis of 25 Pseudomonas spp. strains isolated from soil which produce high-strength surfactants that reduce surface tensions to 25.2 ± 0.1-26.5 ± 0.2 mN.m-1 (the surface tension of sterile growth medium and pure water was 52.9 ± 0.4 mN.m-1 and 72.1 ± 1.2 mN.m-1, respectively)...
January 16, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346420/initial-acquisition-and-succession-of-the-cystic-fibrosis-lung-microbiome-is-associated-with-disease-progression-in-infants-and-preschool-children
#2
Marianne S Muhlebach, Bryan T Zorn, Charles R Esther, Joseph E Hatch, Conor P Murray, Lidija Turkovic, Sarath C Ranganathan, Richard C Boucher, Stephen M Stick, Matthew C Wolfgang
The cystic fibrosis (CF) lung microbiome has been studied in children and adults; however, little is known about its relationship to early disease progression. To better understand the relationship between the lung microbiome and early respiratory disease, we characterized the lower airways microbiome using bronchoalveolar lavage (BAL) samples obtained from clinically stable CF infants and preschoolers who underwent bronchoscopy and chest computed tomography (CT). Cross-sectional samples suggested a progression of the lower airways microbiome with age, beginning with relatively sterile airways in infancy...
January 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29346340/disparities-in-preconception-health-indicators-%C3%A2-behavioral-risk-factor-surveillance-system-2013-2015-and-pregnancy-risk-assessment-monitoring-system-2013-2014
#3
Cheryl Robbins, Sheree L Boulet, Isabel Morgan, Denise V D'Angelo, Lauren B Zapata, Brian Morrow, Andrea Sharma, Charlan D Kroelinger
PROBLEM/CONDITION: Preconception health is a broad term that encompasses the overall health of nonpregnant women during their reproductive years (defined here as aged 18-44 years). Improvement of both birth outcomes and the woman's health occurs when preconception health is optimized. Improving preconception health before and between pregnancies is critical for reducing maternal and infant mortality and pregnancy-related complications. The National Preconception Health and Health Care Initiative's Surveillance and Research work group suggests ten prioritized indicators that states can use to monitor programs or activities for improving the preconception health status of women of reproductive age...
January 19, 2018: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/29346046/ultrasonographic-characteristics-of-the-reproductive-tract-and-serum-progesterone-and-estradiol-concentrations-in-captive-female-red-wolves-canis-rufus-with-and-without-reproductive-tract-disease
#4
Kadie M Anderson, Mandi W Schook, Karen L Goodrowe, William T Waddell, Karen N Wolf
OBJECTIVE To describe ultrasonographic characteristics of the reproductive tract and serum progesterone and estradiol concentrations in captive female red wolves (Canis rufus) with and without reproductive tract disease. DESIGN Prospective study. ANIMALS 13 adult female red wolves. PROCEDURES Wolves with varying parity and history of contraceptive treatment were anesthetized to facilitate ultrasonographic examination and measurement of the reproductive tract and blood collection for determination of serum progesterone and estradiol concentrations in December 2011 and June 2012...
February 1, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/29345577/probiotics-and-paraprobiotics-in-viral-infection-clinical-application-and-effects-on-the-innate-and-acquired-immune-systems
#5
Osamu Kanauchi, Akira Andoh, Sazaly AbuBakar, Naoki Yamamoto
Recently, the risk of viral infection has dramatically increased owing to changes in human ecology such as global warming and an increased geographical movement of people and goods. However, the efficacy of vaccines and remedies for infectious diseases is limited by the high mutation rates of viruses, especially, RNA viruses. Here, we comprehensively review the effectiveness of several probiotics and paraprobiotics (sterilized probiotics) for the prevention or treatment of virally-induced infectious diseases...
January 16, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29345196/surfactant-protein-c-dampens-inflammation-by-decreasing-jak-stat-activation-during-lung-repair
#6
Huiyan Jin, Andrzej K Ciechanowicz, Alanna R Kaplan, Lin Wang, Ping Xia Zhang, Yi-Chien Lu, Rachel E Tobin, Brooke A Tobin, Lauren Cohn, Caroline J Zeiss, Patty J Lee, Emanuela M Bruscia, Diane S Krause
Surfactant Protein C (SPC), a key component of pulmonary surfactant, also plays a role in regulating inflammation. SPC deficiency in patients and mouse models is associated with increased inflammation and delayed repair, but the key drivers of SPC-regulated inflammation in response to injury are largely unknown. This study focuses on a new mechanism of SPC as an anti-inflammatory molecule using SPC-TK/SPC-KO (surfactant protein C-thymidine kinase/surfactant protein C knockout) mice, which represent a novel sterile injury model that mimics clinical acute respiratory distress syndrome (ARDS)...
January 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29344973/use-of-hysterosalpingo-foam-sonography-for-assessment-of-the-efficacy-of-essure-hysteroscopic-sterilization
#7
Maja Rosič, Branka Žegura, Sabina Vadnjal Đonlagić
OBJECTIVES: Hysterosalpingo-foam sonography (HyFoSy) has been suggested to be a possible less invasive alternative to hysterosalpingography (HSG), which is the reference standard for confirmation of tubal occlusion after Essure (Bayer AG, Leverkusen, Germany) hysteroscopic sterilization. The purpose of our study was to evaluate the accuracy of HyFoSy compared to HSG for confirmation of tubal occlusion after Essure hysteroscopic sterilization. METHODS: A prospective study included 90 patients who underwent Essure hysteroscopic sterilization...
January 18, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/29344015/implementation-of-the-single-european-code-in-a-multi-tissue-bank
#8
REVIEW
Jan Schroeter, Tino Schulz, Bernard Schroeter, Katrin Fleischhauer, Axel Pruß
Introduction: The traceability of tissue and cells transplants is important to ensure a high level of safety for the recipients. With the final introduction of the Single European Code (SEC) in April 2017 in the EU a consistent system among all member states became mandatory. Methods: The regulations for the SEC on EU and national level were evaluated. An overview on the different parts of the SEC with detailed explanations is given. Our own experiences with the implementation of the SEC in our multi-tissue bank are reported in addition...
November 2017: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/29343826/mammalian-sterile-20-kinase-1-and-2-are-important-regulators-of-hematopoietic-stem-cells-in-stress-condition
#9
Da-Hye Lee, Tae-Shin Kim, Dongjun Lee, Dae-Sik Lim
The mammalian Hippo signaling pathway has been implicated in the self-renewal and differentiation of stem and progenitor cells. MST1 and MST2 (MST1/2) are core serine-threonine kinases in the Hippo signaling pathway, one of which, MST1, has been extensively investigated for its role in T cell and myeloid cell function. These studies have identified MST1 as a promising therapeutic target in immunological disease. However, the roles of MST1/2 in hematopoietic stem cell (HSC) function in vivo are not fully understood...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343620/comparative-extractables-study-of-autoclavable-polyethersulfone-filter-cartridges-for-sterile-filtration
#10
Roberto Menzel, Ina Pahl, Thomas Loewe, Armin Hauk
Sterile filters are ubiquitous in biopharmaceutical manufacturing processes. Since such filters are in direct contact with the process fluid, profiling of the extractables is of utmost importance. The work presented here reveals the extractables profile from filter cartridges for sterilizing grade filtration, which were obtained from six different vendors. All filters contain a 0.2 μm polyethersulfone (PES) membrane for sterile filtration combined with a PES pre-filter with retention rates spanning from 0...
January 17, 2018: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29343618/retention-of-acholeplasma-laidlawii-by-sterile-filtration-membranes-effect-of-cultivation-medium-and-filtration-temperature
#11
Alexander Helling, Hannes König, Felix Seiler, Ralph Berkholz, Volkmar Thom, Milan Polakovic
This experimental study compares cell size, zeta potential and the ability to penetrate tailor-made size exclusion membrane filters of mycoplasma A. laidlawii cultivated in five different cultivation media. The influence of relevant filtration process parameters, in particular transmembrane pressure and filtration temperature, on their respective retention was tested. The impact of the filtration temperature was further evaluated for the Gram-negative bacteria species Brevundimonas diminuta, the Gram-positive bacteria species Staphylococcus epidermidis, the Pseudomonas phage PP7 and the mycoplasma species M...
January 17, 2018: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29343564/phenotypic-plasticity-in-an-ant-with-strong-caste-genotype-association
#12
Alexandre Kuhn, Hugo Darras, Serge Aron
Caste determination in social Hymenoptera (whether a female egg develops into a reproductive queen or a sterile worker) is a remarkable example of phenotypic plasticity where females with highly similar genomes exhibit striking differences in morphology and behaviour. This phenotypic dichotomy is typically influenced by environmental factors. However, recent studies have revealed a strong caste-genotype association in hybridogenetic ants: workers are all interlineage hybrids while queens are all purebred, suggesting that female caste fate is genetically determined...
January 2018: Biology Letters
https://www.readbyqxmd.com/read/29343307/informed-choice-and-female-sterilization-in-south-asia-and-latin-america
#13
Apoorva Jadhav, Emily Vala-Haynes
Globally, female sterilization is one of the most popular contraceptive methods despite concerns about quality of care for women who report being sterilized. In this study, informed choice among sterilized women was quantified using Demographic and Health Survey (DHS) data from 2000 to 2012 for countries in South Asia and Latin America. Three responses measured informed choice and knowledge about whether women were informed by a health worker or provider: that sterilization is permanent, the potential side-effects of sterilization and other methods of contraception...
January 18, 2018: Journal of Biosocial Science
https://www.readbyqxmd.com/read/29342871/interferons-reprogramming-the-metabolic-network-against-viral-infection
#14
REVIEW
Kavita Raniga, Chen Liang
Viruses exploit the host and induce drastic metabolic changes to ensure an optimal environment for replication and the production of viral progenies. In response, the host has developed diverse countermeasures to sense and limit these alterations to combat viral infection. One such host mechanism is through interferon signaling. Interferons are cytokines that enhances the transcription of hundreds of interferon-stimulated genes (ISGs) whose products are key players in the innate immune response to viral infection...
January 13, 2018: Viruses
https://www.readbyqxmd.com/read/29342487/-application-of-intrabronchial-valves-in-high-risk-patients-with-bronchopleural-fistula-an-alternative-therapeutic-option
#15
Ali Akil, Jan Reichelt, Stefan Freermann, Ilias Karfis, Michael Semik, Stefan Fischer
OBJECTIVES: Persistent air leak or bronchopleural fistula (BPF) is a challenging and frequently observed problem after pulmonary resection and may lead to prolonged chest tube therapy. One efficient nonsurgical approach to manage such a fistula is the application of intrabronchial valves. This may support earlier chest tube removal and hospital discharge. METHODS: Between 04/2015 and 03/2017, n = 8 patients (n = 4 female) with severe pleural empyema and necrotising lung tissue defects developed prolonged air leak and persistent BPF after surgery...
January 17, 2018: Zentralblatt Für Chirurgie
https://www.readbyqxmd.com/read/29341922/sustained-release-timolol-maleate-loaded-ocusert-based-on-biopolymer-composite
#16
Revathy V Nair, Shefrin S, Akhil Suresh, Anoop K R, Sreeja C Nair
In the present investigation, the effect of timolol maleate loaded ocuserts was studied as an alternative for conventional anti-glaucoma formulation. Ocuserts were prepared using natural polymer sodium alginate and ethyl cellulose. Physico-chemical properties along with drug entrapment efficiency (94-98%), content uniformity (93.1% ± 0.264-98.00% ± 0.321), in vitro drug release (83.42% ± 0.35 at end of 12 hours), ex vivo permeation all showed satisfactory results, which was found to follow zero order kinetics...
January 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29341390/building-a-new-research-framework-for-social-evolution-intralocus-caste-antagonism
#17
Tanya M Pennell, Luke Holman, Edward H Morrow, Jeremy Field
The breeding and non-breeding 'castes' of eusocial insects provide a striking example of role-specific selection, where each caste maximises fitness through different morphological, behavioural and physiological trait values. Typically, queens are long-lived egg-layers, while workers are short-lived, largely sterile foragers. Remarkably, the two castes are nevertheless produced by the same genome. The existence of inter-caste genetic correlations is a neglected consequence of this shared genome, potentially hindering the evolution of caste dimorphism: alleles that increase the productivity of queens may decrease the productivity of workers and vice versa, such that each caste is prevented from reaching optimal trait values...
January 16, 2018: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/29341381/effect-of-ethanol-on-the-antimicrobial-properties-of-chlorhexidine-over-oral-biofilm
#18
Pedro Henrique Marks Duarte, Paula Barcellos da Silva, Ricardo Abreu da Rosa, Francisco Montagner, Marco Antônio Húngaro Duarte, Milton Carlos Kuga, Marcus Vinícius Reis Só
The aim of this study was to evaluate the effect of 95% ethanol irrigation, with 5 or 10 min of action, on the antibacterial properties of 2% chlorhexidine (CHX), on oral biofilm, evaluated with confocal laser scanning microscopy (CLSM). Oral biofilm development was induced in 80 sterilized bovine dentin blocks, distributed in two groups (5 or 10 min) and 4 subgroups, according to time and the solution used: Saline (SALINE5, SALINE10); Saline followed by CHX (SALINE/CHX5, SALINE/CHX10); Ethanol (ETHANOL5, ETHANOL10), Ethanol followed by CHX (ETHANOL/CHX5, ETHANOL/CHX10)...
January 17, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29341338/human-teeth-biobank-microbiological-analysis-of-the-teeth-storage-solution
#19
Fabiana Almeida Curylofo-Zotti, Francine Lorencetti-Silva, Jéssica de Almeida Coelho, Rachel Maciel Monteiro, Evandro Watanabe, Silmara Aparecida Milori Corona
The cross-infections may occur during handling of dental elements, affecting the health of dental practitioners and researchers. This study aimed to analyze the influence of the storage medium temperature on the bacterial contingent of the human teeth used for research purposes. Thirty human teeth were donated to the Human Teeth Biobank immediately after extraction. The teeth were cleaned with tap water and neutral soap. The teeth were randomly distributed according to the temperature of the storage solution (deionized water): at 4 °C (refrigerator) or at -10 °C (freezer) and were stored individually in sterile vials during 60 days...
January 17, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29340931/vascutrainer-a-mobile-and-disposable-bioreactor-system-for-the-conditioning-of-tissue-engineered-vascular-grafts
#20
Frederic Wolf, Diana M Rojas González, Ulrich Steinseifer, Markus Obdenbusch, Werner Herfs, Christian Brecher, Stefan Jockenhoevel, Petra Mela, Thomas Schmitz-Rode
In vitro tissue engineering of vascular grafts requires dynamic conditioning in a bioreactor system for in vitro tissue maturation and remodeling to receive a mechanically adequate and hemocompatible implant. The goal of the current work was to develop a bioreactor system for the conditioning of vascular grafts which is (i) able to create a wide range of flow, pressure and frequency conditions, including physiological ones; (ii) compact and easy to assemble; (iii) transportable; (iv) disposable. The system is driven by a small centrifugal pump controlled via a custom-made control unit, which can also be operated on batteries to allow for autonomous transportation...
January 16, 2018: Annals of Biomedical Engineering
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