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https://www.readbyqxmd.com/read/28738510/degradation-kinetics-and-mechanism-of-sulfadiazine-and-sulfamethoxazole-in-an-agricultural-soil-system-with-manure-application
#1
Yu Zhang, Shuangqing Hu, Hongchang Zhang, Genxiang Shen, Zhejun Yuan, Wei Zhang
Recently, under the application of waste-water, manure and biosolids, antibiotics have been used massively in agriculture resulted in antibiotic resistance and potential environmental risks. In the present study, the removal of sulfadiazine (SDZ) and sulfamethoxazole (SMX) in an agricultural soil system was explored. All the experiments were conducted under different incubation conditions for 49days. The experimental results indicated that all the degradation processes could effectively follow a first-order kinetic model...
July 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28737858/the-effects-of-pneumococcal-conjugate-vaccine-pcv7-and-pcv13-on-turkish-children-with-invasive-pneumococcal-disease-a-single-center-experience
#2
Halil Özdemir, Caner Yıldız, Selin Nar Ötgün, Hatice Erkol, Adem Karbuz, Bilge Aldemir Kocabaş, Tuğçe Tural Kara, Ayşegül Gözalan, Rıza Durmaz, Ergin Çiftçi, Derya Aysev, Erdal İnce
INTRODUCTION: The aim of this prospective single-center study was to determine the changings in incidence of invasive pneumococcal disease (IPD), serotype distribution and the antimicrobial resistance patterns of S. pneumoniae in children with IPD after the period (1 to 7 years) of vaccination with PCV7 (2008) and PCV13 (2011). POPULATION AND METHODS: The study was conducted on 39 Turkish children with IPD between ages 1 month and 18 years in Ankara, Turkey. Streptococcus pneumoniae was identified using standard laboratory procedures from blood, cerebrospinal fluid (CSF), pleural fluid, and other sterile body fluids and tissues...
August 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28737465/the-effect-of-ozone-gas-sterilization-on-the-properties-and-cell-compatibility-of-electrospun-polycaprolactone-scaffolds
#3
Carolina Fracalossi Rediguieri, Paul A De Bank, Maria Helena Ambrosio Zanin, Patrícia Leo, Natalia Neto Pereira Cerize, Adriano Marim de Oliveira, Terezinha de Jesus Andreoli Pinto
The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in this capacity, these medical devices need to be sterile. Traditional methods of sterilization are not always suitable for biomaterials, especially as many commonly used biomedical polymers are sensitive to chemical-, thermal- or radiation-induced damage...
July 24, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28736794/tinea-capitis-by-microsporum-audouinii-case-reports-and-review-of-published-global-literature-2000-2016
#4
Fábio Brito-Santos, Maria Helena Galdino Figueiredo-Carvalho, Rowena Alves Coelho, Anna Sales, Rodrigo Almeida-Paes
Tinea capitis caused by Microsporum audouinii is reported herein from two Brazilian schoolchildren, which are brothers. Arthroconidia were evidenced on direct examination of scalp hair, and a fungus of the genus Microsporum was isolated from cultures of each patient. The isolated fungi were classified as M. audouinii by visualization of species-specific structures, including: pectinate hyphae, chlamydospores, and fusiform macroconidia, sterile growth with characteristic brown pigment in rice grains, and through DNA sequencing of the internal transcriber spacer region...
July 24, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28736517/resolution-of-cochlear-inflammation-novel-target-for-preventing-or-ameliorating-drug-noise-and-age-related-hearing-loss
#5
REVIEW
Gilda M Kalinec, Gwen Lomberk, Raul A Urrutia, Federico Kalinec
A significant number of studies support the idea that inflammatory responses are intimately associated with drug-, noise- and age-related hearing loss (DRHL, NRHL and ARHL). Consequently, several clinical strategies aimed at reducing auditory dysfunction by preventing inflammation are currently under intense scrutiny. Inflammation, however, is a normal adaptive response aimed at restoring tissue functionality and homeostasis after infection, tissue injury and even stress under sterile conditions, and suppressing it could have unintended negative consequences...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28736264/mesenchymal-stem-stromal-cell-extracellular-vesicles-from-active-principle-to-next-generation-drug-delivery-system
#6
REVIEW
Barbara Crivelli, Theodora Chlapanidas, Sara Perteghella, Enrico Lucarelli, Luisa Pascucci, Anna Teresa Brini, Ivana Ferrero, Mario Marazzi, Augusto Pessina, Maria Luisa Torre
It has been demonstrated that the biological effector of mesenchymal stem/stromal cells (MSCs) is their secretome, which is composed of a heterogeneous pool of bioactive molecules, partially enclosed in extracellular vesicles (EVs). Therefore, the MSC secretome (including EVs) has been recently proposed as possible alternative to MSC therapy. The secretome can be considered as a protein-based biotechnological product, it is probably safer compared with living/cycling cells, it presents virtually lower tumorigenic risk, and it can be handled, stored and sterilized as an Active Pharmaceutical/Principle Ingredient (API)...
July 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28736026/yogurt-feeding-induced-the-prolongation-of-fully-major-histocompatibility-complex-mismatched-murine-cardiac-graft-survival-by-induction-of-cd4-foxp3-cells
#7
M Uchiyama, E Yin, T Yanagisawa, X Jin, M Hara, S Matsuyama, T Imazuru, K Uchida, M Kawamura, M Niimi
Yogurt is a nutrient-rich food and the beneficial effects of yogurt on both health and immunomodulatory effects are well documented. In this pilot study, we investigated the effects of commercially produced yogurt R-1 on alloimmune responses in a murine cardiac transplantation model. The R-1 is produced by Meiji Co., Ltd., and contains live and active lactic acid bacteria (lactobacillus bulgaricus OLL1073R-1) mainly. CBA (H2(k)) mice underwent transplantation of a C57BL/6 (H2(b); B6) heart and received oral administration of 1 mL, 0...
July 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28735999/results-of-real-time-multiplex-polymerase-chain-reaction-assay-in-renal-transplant-recipients-with-sterile-pyuria
#8
M Sarier, M Demir, S Goktas, I Duman, M Buyukkinaci, Y Yuksel, S Tekin, A H Yavuz, A Sengul
Urinary tract infections are a major cause of morbidity and hospitalization after renal transplantation. Patients treated with immunosuppressive drugs suffer not only from common uropathogens but also from opportunistic infections caused by unusual uropathogens. Sterile pyuria is associated with numerous infectious agents including viruses, fungi, and atypical or fastidious organisms. The objective of this study was to investigate the pathogens using real-time multiplex polymerase chain reaction (rtMPCR) assay in sterile pyuria of renal transplant recipients...
July 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28735270/subcutaneous-levetiracetam-for-the-management-of-seizures-at-the-end-of-life
#9
REVIEW
Anna Elizabeth Sutherland, John Curtin, Victoria Bradley, Olivia Bush, Maggie Presswood, Victoria Hedges, Katrien Naessens
OBJECTIVES: To report the results of a combined case series analysis of subcutaneous levetiracetam (Keppra) for the management of seizures in palliative care patients. METHODS: A comprehensive literature review on the use of subcutaneous levetiracetam was performed, and these data were combined with a prospective observational audit of its use in terminal care undertaken in a regional palliative care network. RESULTS: 7 papers were identified from the literature review-four case reports and three observational case series-reporting on a total of 53 cases where subcutaneous levetiracetam was administered...
July 22, 2017: BMJ Supportive & Palliative Care
https://www.readbyqxmd.com/read/28733712/fertility-and-ploidy-of-gametes-of-allodiploid-and-allotriploid-loaches-produced-by-diploid-misgurnus-anguillicaudatus-females-and-paramisgurnus-dabryanus-males
#10
Songqian Huang, Xiaojuan Cao, Weimin Wang, Mona Nasr
Artificial and natural hybridization of dojo loach Misgurnus anguillicaudatus (2N = 50, DD for short) and large-scale loach Paramisgurnus dabryanus (2N = 48, PP for short) are well-grown. However, these hybrid loaches have not yet been examined for fertility and ploidy of gametes. Here, histological observations, artificial propagation, observations of embryonic development, larval morphology, and ploidy analyses were conducted to determine the fertility and ploidy of gametes of allodiploid (DP for short) and allotriploid (DDP for short) loaches, produced by DD females × PP males and induced from fertilized eggs of DD females × PP males by cold shock to prevent the second polar body release, respectively...
July 21, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28733577/skin-renewal-activity-of-non-thermal-plasma-through-the-activation-of-%C3%AE-catenin-in-keratinocytes
#11
J H Choi, Y S Song, K Song, H J Lee, J W Hong, G C Kim
For recent years, devices that generate non-thermal plasma (NTP) have been introduced into the field of dermatology. Since NTP has demonstrated strong anti-pathogenic activity with safety of use, NTP was first applied to sterilize the skin surface to aid in the healing of various kinds of skin diseases. However, the effect of NTP on skin regeneration has not yet been fully explored. In this study, the effect of NTP on the growth of keratinocytes was tested using the HaCaT human keratinocyte cell line and HRM2 hairless mice...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28732890/effects-of-supercritical-carbon-dioxide-sterilization-on-polysaccharidic-membranes-for-surgical-applications
#12
F Scognamiglio, M Blanchy, M Borgogna, A Travan, I Donati, J W A M Bosmans, M P Foulc, N D Bouvy, S Paoletti, E Marsich
Sterilization methods such as ɣ-irradiation, steam sterilization and ethylene oxide gas treatment can have negative effects on molecular structure and properties of polysaccharide-based biomaterials. In this perspective, the use of supercritical carbon dioxide (scCO2) has been proposed as an alternative method for biomaterial sterilization. In this work, chemical, mechanical and biological properties of polysaccharidic membranes for surgical applications were investigated after sterilization by scCO2. Four sets of sterilizing conditions were considered and SEC analyses were performed in order to identify the one with lower impact on the polysaccharidic matrix of membranes (alginate)...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28732688/molecular-pathophysiology-of-gout
#13
REVIEW
Jyaysi Desai, Stefanie Steiger, Hans-Joachim Anders
Three contradictory clinical presentations of gout have puzzled clinicians and basic scientists for some time: first, the crescendo of sterile inflammation in acute gouty arthritis; second, its spontaneous resolution, despite monosodium urate (MSU) crystal persistence in the synovium; and third, immune anergy to MSU crystal masses observed in tophaceous or visceral gout. Here, we provide an update on the molecular pathophysiology of these gout manifestations, namely, how MSU crystals can trigger the auto-amplification loop of necroinflammation underlying the crescendo of acute gouty arthritis...
July 18, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28732083/apol1-risk-alleles-among-individuals-with-ckd-in-northern-tanzania-a-pilot-study
#14
John W Stanifer, Francis Karia, Venance Maro, Kajiru Kilonzo, Xuejun Qin, Uptal D Patel, Elizabeth R Hauser
INTRODUCTION: In sub-Saharan Africa, approximately 100 million people have CKD, yet genetic risk factors are not well-understood. Despite the potential importance of understanding APOL1 risk allele status among individuals with CKD, little genetic research has been conducted. Therefore, we conducted a pilot study evaluating the feasibility of and willingness to participate in genetic research on kidney disease, and we estimated APOL1 risk allele frequencies among individuals with CKD...
2017: PloS One
https://www.readbyqxmd.com/read/28732077/mutations-in-the-caenorhabditis-elegans-orthologs-of-human-genes-required-for-mitochondrial-trna-modification-cause-similar-electron-transport-chain-defects-but-different-nuclear-responses
#15
Carmen Navarro-González, Ismaïl Moukadiri, Magda Villarroya, Ernesto López-Pascual, Simon Tuck, M-Eugenia Armengod
Several oxidative phosphorylation (OXPHOS) diseases are caused by defects in the post-transcriptional modification of mitochondrial tRNAs (mt-tRNAs). Mutations in MTO1 or GTPBP3 impair the modification of the wobble uridine at position 5 of the pyrimidine ring and cause heart failure. Mutations in TRMU affect modification at position 2 and cause liver disease. Presently, the molecular basis of the diseases and why mutations in the different genes lead to such different clinical symptoms is poorly understood...
July 21, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28731486/demineralized-dentin-and-enamel-matrices-as-suitable-substrates-for-bone-regeneration
#16
Nina Bono, Paolo Tarsini, Gabriele Candiani
BACKGROUND: In recent decades, tooth derivatives such as dentin (D) and enamel (E) have been considered as potential graft biomaterials to treat bone defects. This study aimed to investigate the effects of demineralization on the physical-chemical and biological behavior of D and E. METHODS: Human D and E were minced into particles (Ø<1 mm), demineralized and sterilized. Thorough physical-chemical and biochemical characterizations of native and demineralized materials were performed by SEM and EDS analysis and ELISA kits to determine mineral, collagen type I and BMP-2 contents...
July 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28731265/application-of-dead-end-knockout-zebrafish-as-recipients-of-germ-cell-transplantation
#17
Qian Li, Wataru Fujii, Kunihiko Naito, Goro Yoshizaki
Germ cell transplantation is a promising technology for the propagation of endangered or valuable fishes. In this technique, sterile male and female recipient fish are injected with donor germ cells so they can produce viable gametes derived from the donor cells. The dead end (dnd) gene is involved in the migration of primordial germ cells; therefore, dnd-knockout zebrafish are expected to be germ-cell-free, making them suitable recipients for germ cell transplantation. dnd mutants were produced by microinjecting 2 nl of 10 ng/μl cRNAs encoding zinc finger nucleases against dnd into the blastodisc of zebrafish embryos before the cell cleavage stage...
July 21, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28730545/platelet-first-responders-in-wound-response-cancer-and-metastasis
#18
REVIEW
David G Menter, Scott Kopetz, Ernest Hawk, Anil K Sood, Jonathan M Loree, Paolo Gresele, Kenneth V Honn
Platelets serve as "first responders" during normal wounding and homeostasis. Arising from bone marrow stem cell lineage megakaryocytes, anucleate platelets can influence inflammation and immune regulation. Biophysically, platelets are optimized due to size and discoid morphology to distribute near vessel walls, monitor vascular integrity, and initiate quick responses to vascular lesions. Adhesion receptors linked to a highly reactive filopodia-generating cytoskeleton maximizes their vascular surface contact allowing rapid response capabilities...
July 20, 2017: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/28729970/severe-cyclophosphamide-related-hyponatremia-in-a-patient-with-acute-glomerulonephritis
#19
Pasquale Esposito, Maria Valentina Domenech, Nicoletta Serpieri, Marta Calatroni, Ilaria Massa, Alessandro Avella, Edoardo La Porta, Luca Estienne, Elena Caramella, Teresa Rampino
Cyclophosphamide is frequently used to treat cancer, autoimmune and renal diseases, such as rapidly progressive glomerulonephritis. Its side effects are well-known, including bone marrow depression, infections, alopecia, sterility, bladder malignancy and hemorrhagic cystitis. Moreover, in some cases cyclophosphamide use has been related to the onset of hyponatremia, by development of a syndrome of inappropriate antidiuresis. Indeed, severe hyponatremia has been previously reported in patients treated with high-dose or moderate-dose of intravenous cyclophosphamide, while only few cases have been reported in patients treated with low dose...
July 6, 2017: World Journal of Nephrology
https://www.readbyqxmd.com/read/28729791/correlation-between-salivary-cortisol-levels-and-dental-anxiety-in-children-of-smokers-and-nonsmokers
#20
S Pavani Reddy, M Ghanashyam Prasad, A Naga RadhaKrishna, Kaniti Saujanya, N V K Raviteja, B Deepthi
OBJECTIVES: The present study was undertaken to evaluate salivary cortisol levels in children of smokers and nonsmokers and thereby establishing the relationship between cortisol levels in response to anxiety in children based on their father's habit of smoking. MATERIALS AND METHODS: The study population aged between 8 and 10 years includes two groups. Group 1 is comprised 20 children of cigarette smokers and Group 2 is comprised 20 children of nonsmokers. The passive drooling technique was used to collect unstimulated saliva from the children using a sterile container...
April 2017: European Journal of Dentistry
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