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https://www.readbyqxmd.com/read/28926878/-effects-of-arnebia-root-oil-on-wound-healing-of-rats-with-full-thickness-skin-defect-and-the-related-mechanism
#1
J Y Shen, Q Ma, Z B Yang, J J Gong, Y S Wu
Objective: To observe the effects of arnebia root oil on wound healing of rats with full-thickness skin defect, and to explore the related mechanism. Methods: Eighty SD rats were divided into arnebia root oil group and control group according to the random number table, with 40 rats in each group, then full-thickness skin wounds with area of 3 cm×3 cm were inflicted on the back of each rat. Wounds of rats in arnebia root oil group and control group were treated with sterile medical gauze and bandage package infiltrated with arnebia root oil gauze or Vaseline gauze, respectively, with dressing change of once every two days...
September 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28925526/sub1-has-photoreceptor-dependent-and-independent-functions-in-sexual-development-and-secondary-metabolism-in-trichoderma-reesei
#2
Hoda Bazafkan, Sabrina Beier, Eva Stappler, Stefan Böhmdorfer, Josua T Oberlerchner, Michael Sulyok, Monika Schmoll
Light dependent processes are involved in the regulation of growth, development and enzyme production in Trichoderma reesei. The photoreceptors BLR1, BLR2 and ENV1 exert crucial functions in these processes. We analyzed the involvement of the transcription factor SUB1 in sexual development as well as secondary metabolism and its position in the light signaling cascade. SUB1 influences growth and in contrast to its homologue in N. crassa, SUB1 is not essential for fruiting body formation and male fertility in T...
September 19, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28925491/corrosion-behavior-of-dental-implants-immersed-into-human-saliva-preliminary-results-of-an-in-vitro-study
#3
M Barbieri, F Mencio, P Papi, D Rosella, S Di Carlo, T Valente, G Pompa
OBJECTIVE: Over the years, different implant surfaces have been used to try to maximize bone to implant contact. The aim of this study was to compare levels of metallic ions and particles dissolution collected from two different dental implants surfaces immersed into human saliva. PATIENTS AND METHODS: A total of 60 dental implants were tested. Group A: sanded with aluminium oxide medium grade particles and acid-etched; Group B: micro-sanded with calcium phosphate powders and acid-etched...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28924140/linoleic-and-palmitoleic-acid-block-streptokinase-mediated-plasminogen-activation-and-reduce-severity-of-invasive-group-a-streptococcal-infection
#4
Katharina Rox, Rolf Jansen, Torsten G Loof, Christine M Gillen, Steffen Bernecker, Mark J Walker, Gursharan Singh Chhatwal, Rolf Müller
In contrast to mild infections of Group A Streptococcus (GAS) invasive infections of GAS still pose a serious health hazard: GAS disseminates from sterile sites into the blood stream or deep tissues and causes sepsis or necrotizing fasciitis. In this case antibiotics do not provide an effective cure as the bacteria are capable to hide from them very quickly. Therefore, new remedies are urgently needed. Starting from a myxobacterial natural products screening campaign, we identified two fatty acids isolated from myxobacteria, linoleic and palmitoleic acid, specifically blocking streptokinase-mediated activation of plasminogen and thereby preventing streptococci from hijacking the host's plasminogen/plasmin system...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923525/morphological-and-functional-characterization-of-the-hemocytes-from-the-pearl-oyster-pteria-hirundo-and-their-immune-responses-against-vibrio-infections
#5
Graziela Cleuza Vieira, Patrícia Mirella da Silva, Margherita Anna Barracco, Augusto Ferrari Hering, Marcos Caivano Pedroso de Albuquerque, Jaqueline da Rosa Coelho, Éder Carlos Schmidt, Zenilda Laurita Bouzon, Rafael Diego Rosa, Luciane Maria Perazzolo
Hemocyte populations of the pearl oyster Pteria hirundo were characterized at morphological, ultrastructural and functional levels. Three main hemocyte populations were identified: hyalinocytes, granulocytes and blast-like cells. Hyalinocytes were the most abundant population (88.2%) characterized by the presence of few or no granules in the cytoplasm and composed by two subpopulations, large and small hyalinocytes. Comparatively, granulocytes represented 2.2% of the hemocyte population and were characterized by the presence of numerous large electron-lucid granules in the cytoplasm...
September 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28923067/a-new-insight-into-male-fertility-preservation-for-patients-with-completely-immotile-spermatozoa
#6
Huanhua Chen, Guixue Feng, Bo Zhang, Hong Zhou, Caizhu Wang, Jinhui Shu, Xianyou Gan, Ruoyun Lin, Dongmei Huang, Yingqin Huang
BACKGROUND: Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for future assisted reproductive treatments. Many studies have shown that successful fertilization can be obtained after selection of viable sperm from the completely immotile spermatozoa before ICSI...
September 18, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28922990/streamlined-system-for-conducting-in-vitro-studies-using-decellularized-kidney-scaffolds
#7
Andrew Reyes Padalhin, Chanmi Park, Byong-Taek Lee
Kidney regeneration is a complex process that can only be studied in vitro at a limited capacity due to the inherent structural and functional complexity of its tissues. Thus, a suitable platform for conducting cellular response and development should be established from decellularized tissues with intact microarchitecture. In this study, a modular streamlined system was developed to allow manageable handling and set-up of in vitro studies using decellularized rat kidneys. The system is composed of commercially available parts that can be reused, interchanged, and re-configured based on the desired experimental stage and process...
September 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28922773/crop-model-assisted-phenomics-and-genome-wide-association-study-for-climate-adaptation-of-indica-rice-2-thermal-stress-and-spikelet-sterility
#8
Michael Dingkuhn, Richard Pasco, Julie Mae Pasuquin, Jean Damo, Jean-Christophe Soulié, Louis-Marie Raboin, Julie Dusserre, Abdoulaye Sow, Baboucarr Manneh, Suchit Shrestha, Tobias Kretzschmar
Low night and high day temperatures during sensitive reproductive stages cause spikelet sterility in rice. Phenotyping of tolerance traits in the field is difficult because of temporal interactions with phenology and organ temperature differing from ambient. Physiological models can be used to separate these effects. A 203-accession indica rice diversity panel was phenotyped for sterility in ten environments in Senegal and Madagascar and climate data were recorded. Here we report on sterility responses while a companion study reported on phenology...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922764/a-pentatricopeptide-repeat-protein-restores-nap-cytoplasmic-male-sterility-in-brassica-napus
#9
Zhi Liu, Faming Dong, Xiang Wang, Tao Wang, Rui Su, Dengfeng Hong, Guangsheng Yang
Two forms of male-sterile cytoplasm, designated nap and pol, are found in the oilseed rape species, Brassica napus. The nap cytoplasm is observed in most B. napus varieties, and it confers male sterility on a limited number of cultivars that lack the corresponding restorer gene, Rfn. In the present study, using linkage analysis in combination with 5652 BC1 progeny derived from a cross between a nap cytoplasmic male sterility (CMS) line 181A and a restorer line H5, we delimited the Rfn gene to a 10.5 kb region on chromosome A09, which contained three putative ORFs...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922615/the-impact-of-incorporating-antimicrobials-into-implant-surfaces
#10
N J Hickok, I M Shapiro, A F Chen
With the increase in numbers of joint replacements, spinal surgeries, and dental implantations, there is an urgent need to combat implant-associated infection. In addition to stringent sterile techniques, an efficacious way to prevent this destructive complication is to create new implants with antimicrobial properties. Specifically, these implants must be active in the dental implant environment where the implant is bathed in the glycoprotein-rich salivary fluids that enhance bacterial adhesion, and propagation, and biofilm formation...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28922285/what-where-and-why-exploring-fluorodeoxyglucose-pet-s-ability-to-localise-and-differentiate-infection-from-cancer
#11
Abby Douglas, Eddie Lau, Karin Thursky, Monica Slavin
PURPOSE OF REVIEW: To review the utility of FDG-PET imaging in detecting the cause of fever and infection in patients with cancer. RECENT FINDINGS: FDG-PET has been shown to have high sensitivity and accuracy for causes of neutropenic fever, leading to higher diagnostic certainty in this group. Recent advances in pathogen-specific labelling in PET to identify Aspergillus spp. and Yersinia spp. infections in mice, as well as differentiating between Gram-positive, Gram-negative and mycobacterial infections are promising...
September 15, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28922027/further-evidence-of-how-unbuffered-starvation-at-4%C3%A2-c-influences-listeria-monocytogenes-egd-e-hcc23-f2365-and-scott-a
#12
Nathan A Jarvis, Corliss A O'Bryan, Elizabeth M Martin, Steven C Ricke, Michael G Johnson, Philip G Crandall
The soilborne pathogen Listeria monocytogenes frequently contaminates food products and food processing environments and is able to survive desiccation, high osmotic pressures, and starvation. However, little is known about how this pathogen survives starvation at 4°C. This study provides evidence that L. monocytogenes is able to survive total nutrient starvation for 4 weeks. L. monocytogenes strains EGD-e, Scott A, F2365, and HCC23 were starved individually in sterile water. Colony counts declined over 4 weeks, with Scott A declining the most rapidly...
September 18, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28920952/mk2-phosphorylation-of-ripk1-regulates-tnf-mediated-cell-death
#13
Yves Dondelinger, Tom Delanghe, Diego Rojas-Rivera, Dario Priem, Tinneke Delvaeye, Inge Bruggeman, Franky Van Herreweghe, Peter Vandenabeele, Mathieu J M Bertrand
TNF is a master proinflammatory cytokine whose pathogenic role in inflammatory disorders can, in certain conditions, be attributed to RIPK1 kinase-dependent cell death. Survival, however, is the default response of most cells to TNF stimulation, indicating that cell demise is normally actively repressed and that specific checkpoints must be turned off for cell death to proceed. We identified RIPK1 as a direct substrate of MK2 in the TNFR1 signalling pathway. Phosphorylation of RIPK1 by MK2 limits cytosolic activation of RIPK1 and the subsequent assembly of the death complex that drives RIPK1 kinase-dependent apoptosis and necroptosis...
September 18, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28920829/allobranchiibius-huperziae-gen-nov-sp-nov-a-member-of-dermacoccaceae-isolated-from-the-root-of-a-medicinal-plant-huperzia-serrata-thunb
#14
Meng-Jie Ai, Ye Sun, Hong-Min Sun, Hong-Yu Liu, Li-Yan Yu, Yu-Qin Zhang
A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.) collected from Sichuan Province, south-west China. The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge. Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates. MK-8(H4) was the only menaquinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920827/marmoricola-endophyticus-sp-nov-an-endophytic-actinobacterium-isolated-from-thespesia-populnea
#15
Zhong-Ke Jiang, Zhen Pan, Fei-Na Li, Xiao-Jun Li, Shao-Wei Liu, Li Tuo, Ming-Guo Jiang, Cheng-Hang Sun
A novel endophytic actinobacterium, designated strain 8BXZ-J1T, was isolated from surface-sterilized branches of Thespesia populnea collected from Beilun Estuary Mangrove Forest National Nature Reserve in Guangxi, China, and examined by a polyphasic approach to determine its taxonomic position. Cells of the isolate were Gram-stain-positive, aerobic, non-spore-forming, non-motile and short rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 8BXZ-J1T belonged to the genus Marmoricola, sharing highest similarity with Marmoricola solisilvae DSM 27140T (96...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920726/the-human-subject-vulnerable-populations-and-medical-history-the-problem-of-presentism-and-the-discourse-of-bioethics
#16
J T H Connor
This discussion considers recent historical works of eugenics and sterilization in Canada, but it is not an historiographic review essay or critique per se of this literature. Rather, by focussing on the topic of historic diagnostic categories such as "feeble-minded," "idiot," and "moron," methodological issues such as historical presentism and its possible interactions with the discourse of modern bioethics are examined. The conclusions derived are meant only to be cautionary, and are neither prescriptive nor proscriptive...
2017: Canadian Bulletin of Medical History, Bulletin Canadien D'histoire de la Médecine
https://www.readbyqxmd.com/read/28920628/fluoroquinolones-as-imaging-agents-for-bacterial-infection
#17
Syed Ali Raza Naqvi, Karl Drlica
Diagnosis of deep-seated bacterial infection is difficult, as neither standard anatomical imaging nor radiolabeled, autologous leukocytes distinguish sterile inflammation from infection. Two recent imaging efforts are receiving attention: (1) radioactive derivatives of sorbitol show good specificity with Gram-negative bacterial infections, and (2) success in combining anatomical and functional imaging for cancer diagnosis has rekindled interest in (99m)Tc-fluoroquinolone-based imaging. With the latter, computed tomography (CT) would be combined with single-photon-emission-computed tomography (SPECT) to detect (99m)Tc-fluoroquinolone-bacterial interactions...
September 18, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28920398/-fermentation-purification-and-immunogenicity-evaluation-of-hepatitis-e-virus-like-particles-expressed-in-hansenula-polymorpha
#18
Caixia Su, Li Li, Zhenji Jin, Xudong Han, Ping Zhao, Lin Wang, Chunhu Jiang, Yueli Wang, Wenwen Wang, Deqi Xu, Naishuo Zhu
To develop a new recombinant hepatitis E vaccine, we used Hansenula polymorpha expression system to express recombinant hepatitis E virus-like particles (HEV VLPs), to construct a recombinant engineered strain HP/HEV2.3. The fermentation conditions and purification process were studied next. The first working seed lots were fermented in liquid culture, and the fermentation products were collected, then crushed, clarified, purified by ultrafiltration, silica gel adsorbed and desorbed, concentrated by ultrafiltration, purified by liquid chromatography and sterilized by filtration...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28920092/thermogelling-properties-of-purified-poloxamer-407
#19
Amir Fakhari, Marta Corcoran, Alexander Schwarz
Poloxamers are triblock copolymers with a center block of hydrophobic polypropylene oxide (PPO) flanked by two hydrophilic polyethyleneoxide (PEO) blocks. Among this family of copolymers, poloxamer 407 is a non-ionic surfactant with reversible gelation properties above a particular polymer concentration and a particular temperature. Easy preparation of poloxamer 407 based sterile injectable formulations have made this copolymer a good candidate for drug delivery, specifically when controlled release of the drug is required...
August 2017: Heliyon
https://www.readbyqxmd.com/read/28920046/the-effect-of-intraoperative-alkali-treatment-on-recovery-from-atracurium-induced-neuromuscular-blockade-in-renal-transplantation-a-randomized-trial
#20
Navid Noraee, Mohammad Fathi, Majid Golestani Eraghi, Ali Dabbagh, Nilofar Massoudi
BACKGROUND: Intraoperative care and anesthesia method in patients undergoing allograft renal transplantation surgery are very necessary. Acid-base imbalance can alter neuromuscular blockade and recovery time. OBJECTIVES: The aim of the present study was to investigate the effect of acid-base balance on atracurium blockade in renal transplantation. METHODS: In this randomized-controlled trial, 31 end-stage renal disease (ESRD) patients undergoing renal transplantation were randomly assigned into two equal groups...
February 2017: Anesthesiology and Pain Medicine
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