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https://www.readbyqxmd.com/read/28655215/development-of-a-new-method-based-on-unmodified-gold-nanoparticles-and-peptide-nucleic-acids-for-detecting-bovine-viral-diarrhea-virus-rna
#1
Maryam Askaravi, Seyedeh Elham Rezatofighi, Saadat Rastegarzadeh, Masoud Reza Seifi Abad Shapouri
A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence of charge-neutral peptide nucleic acids (PNA). Free charge-neutral PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with PNA disrupted PNA-induced AuNP aggregation, and the NPs remained stable. However, non-complementary RNA did not have this effect, and PNA induced aggregation of the AuNPs that resulted in a color change of the reaction from red to blue...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28655161/dysfunction-of-pla2g6-and-cyp2c44-associated-network-signals-imminent-carcinogenesis-from-chronic-inflammation-to-hepatocellular-carcinoma
#2
Meiyi Li, Chen Li, Wei-Xin Liu, Conghui Liu, Jingru Cui, Qingrun Li, Hong Ni, Yingcheng Yang, Chaochao Wu, Chunlei Chen, Xing Zhen, Tao Zeng, Mujun Zhao, Lei Chen, Jiarui Wu, Rong Zeng, Luonan Chen
Little is known about how chronic inflammation contributes to the progression of hepatocellular carcinoma (HCC), especially the initiation of cancer. To uncover the critical transition from chronic inflammation to HCC and the molecular mechanisms at a network level, we analyzed the time-series proteomic data of woodchuck hepatitis virus/c-myc mice and age-matched wt-C57BL/6 mice using our dynamical network biomarker (DNB) model. DNB analysis indicated that the 5th month after birth of transgenic mice was the critical period of cancer initiation, just before the critical transition, which is consistent with clinical symptoms...
June 26, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/28654877/highlighting-the-complexities-of-a-groundwater-pilot-study-during-an-avian-influenza-outbreak-methods-lessons-learned-and-select-contaminant-results
#3
Laura E Hubbard, Dana W Kolpin, Chad L Fields, Michelle L Hladik, Luke R Iwanowicz
The highly pathogenic avian influenza (H5N2) outbreak in the Midwestern United States (US) in 2015 was historic due to the number of birds and poultry operations impacted and the corresponding economic loss to the poultry industry and was the largest animal health emergency in US history. The U.S. Geological Survey (USGS), with the assistance of several state and federal agencies, aided the response to the outbreak by developing a study to determine the extent of virus transport in the environment. The study goals were to: develop the appropriate sampling methods and protocols for measuring avian influenza virus (AIV) in groundwater, provide the first baseline data on AIV and outbreak- and poultry-related contaminant occurrence and movement into groundwater, and document climatological factors that may have affected both survival and transport of AIV to groundwater during the months of the 2015 outbreak...
June 24, 2017: Environmental Research
https://www.readbyqxmd.com/read/28654867/cholesterol-rich-lipid-rafts-play-a-critical-role-in-bovine-parainfluenza-virus-type-3-bpiv3-infection
#4
Liyang Li, Liyun Yu, Xilin Hou
Lipid rafts are specialized lipid domains enriched in cholesterol and sphingolipid, which can be utilized in the lifecycle of numerous enveloped viruses. Bovine parainfluenza virustype3 (BPIV3) entry to cell is mediated by receptor binding and membrane fusion, but how lipid rafts in host cell membrane and BPIV3 envelope affect virus infection remains unclear. In this study, we investigated the role of lipid rafts in the different stages of BPIV3 infection. The MDBK cells were treated by methyl-β-cyclodextrin (MβCD) to disrupt cellular lipid raft, and the virus infection was determined...
May 11, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28654862/biopolymer-zein-coated-gold-nanoparticles-synthesis-antibacterial-potential-toxicity-and-histopathological-effects-against-the-zika-virus-vector-aedes-aegypti
#5
Periyakaruppan Suganya, Baskaralingam Vaseeharan, Sekar Vijayakumar, Banumathi Balan, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
The control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. The present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (Ze-AuNPs) against Gram positive (Bacillus pumilus and Bacillus subtilis), Gram negative (Shigella sonnei and Pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and Zika virus vector Aedes aegypti. The synthesized Ze-AuNPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM)...
June 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28654775/hepatitis-b-virus-reactivation-among-hepatitis-c-patients-treated-with-direct-acting-antiviral-therapies-in-routine-clinical-practice
#6
Elisabetta Loggi, Stefano Gitto, Silvia Galli, Mario Minichiello, Fabio Conti, Elena Grandini, Alessandra Scuteri, Giovanni Vitale, Roberto Di Donato, Carmela Cursaro, Giuliano Furlini, Pietro Andreone
BACKGROUND: Hepatitis B (HBV) reactivation in chronic hepatitis C (CHC) patients treated with IFN-free direct acting antiviral (DAA) therapies has recently emerged as a potential risk. Given the potential burden of this issue, further data are needed to establish its actual clinical impact. OBJECTIVES: The aim of the present study was to analyze the occurrence of HBV reactivation in a cohort of CHC patient treated with DAAs in routine clinical practice. STUDY DESIGN: Consecutive CHC patients with different genotypes, treated with DAA between January 2015 and January 2016 were included in the study...
June 3, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28654766/methotrexate-related-lymphoproliferative-disorder-in-patients-with-osteonecrosis-of-the-jaw-a-3-case-report-and-literature-review
#7
Ken Furudate, Anna Satake, Norihiko Narita, Wataru Kobayashi
Patients with immunodeficiency or immunosuppression are at risk of developing a lymphoproliferative disorder (LPD). Methotrexate (MTX) is an iatrogenic cause of LPD, which in up to 50% cases occurs in extranodal sites. The occurrence of MTX-related LPD with osteonecrosis of the jaw (ONJ) has rarely been reported. Moreover, there are no clear diagnostic criteria and treatment strategies for management of these lesions. In the present cases, discontinuing MTX and debridement of the necrotic bone were effective...
June 1, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28654691/whole-gene-analysis-of-two-groups-of-hepatitis-b-virus-c-d-inter-genotype-recombinant-strains-isolated-in-tibet-china
#8
Tiezhu Liu, Fuzhen Wang, Shuang Zhang, Feng Wang, Qingling Meng, Guomin Zhang, Fuqiang Cui, Dorji Dunzhu, Wenjiao Yin, Shengli Bi, Liping Shen
Tibet is a highly hepatitis B virus (HBV) endemic area. Two types of C/D recombinant HBV are commonly isolated in Tibet and have been previously described. In an effort to better understand the molecular characteristic of these C/D recombinant strains from Tibet, we undertook a multistage random sampling project to collect HBsAg positive samples. Molecular epidemiological and bio-informational technologies were used to analyze the characteristics of the sequences found in this study. There were 60 samples enrolled in the survey, and we obtained 19 whole-genome sequences...
2017: PloS One
https://www.readbyqxmd.com/read/28654687/combined-single-cell-quantitation-of-host-and-siv-genes-and-proteins-ex-vivo-reveals-host-pathogen-interactions-in-individual-cells
#9
Diane L Bolton, Kathleen McGinnis, Greg Finak, Pratip Chattopadhyay, Raphael Gottardo, Mario Roederer
CD4 T cells harboring HIV-1/SIV represent a formidable hurdle to eradicating infection, and yet their detailed phenotype remains unknown. Here we integrate two single-cell technologies, flow cytometry and highly multiplexed quantitative RT-PCR, to characterize SIV-infected CD4 T cells directly ex vivo. Within individual cells, we correlate the cellular phenotype, in terms of host protein and RNA expression, with stages of the viral life cycle defined by combinatorial expression of viral RNAs. Spliced RNA+ infected cells display multiple memory and activation phenotypes, indicating virus production by diverse CD4 T cell subsets...
June 27, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28654678/genetic-factors-affecting-ebv-copy-number-in-lymphoblastoid-cell-lines-derived-from-the-1000-genome-project-samples
#10
Rajendra Mandage, Marco Telford, Juan Antonio Rodríguez, Xavier Farré, Hafid Layouni, Urko M Marigorta, Caitlin Cundiff, Jose Maria Heredia-Genestar, Arcadi Navarro, Gabriel Santpere
Epstein-Barr virus (EBV), human herpes virus 4, has been classically associated with infectious mononucleosis, multiple sclerosis and several types of cancers. Many of these diseases show marked geographical differences in prevalence, which points to underlying genetic and/or environmental factors. Those factors may include a different susceptibility to EBV infection and viral copy number among human populations. Since EBV is commonly used to transform B-cells into lymphoblastoid cell lines (LCLs) we hypothesize that differences in EBV copy number among individual LCLs may reflect differential susceptibility to EBV infection...
2017: PloS One
https://www.readbyqxmd.com/read/28654651/herpes-simplex-virus-1-and-2-educational-assessment-of-young-adults-in-rural-southwest-virginia
#11
Shantal S Hover, Andrea S Bertke
PURPOSE: Herpes simplex virus 2 (HSV-2) causes genital herpes, one of the most common sexually transmitted infections (STIs) in the U.S. HSV-1, commonly associated with cold sores, is increasing as a cause of genital herpes. Abstinence-only sexual health classes, commonly taught in Virginia, generate young adults who are under-educated in sexual health, increasing STI risks. College students in southwest Virginia were surveyed to assess comprehensiveness of high school health education regarding HSV-1 and HSV-2 and to identify students' preferred methods for STI education...
2017: PloS One
https://www.readbyqxmd.com/read/28654650/risk-factors-for-prevalent-hepatitis-c-virus-infection-among-inmates-in-a-state-prison-system-in-mexico
#12
Pablo F Belaunzarán-Zamudio, Juan L Mosqueda-Gomez, Alejando Macias-Hernandez, Juan G Sierra-Madero, Saifuddin Ahmed, Chris Beyrer
OBJECTIVES: To estimate the prevalence of HCV-infection and identify associated factors among inmates in the State Prison System of Guanajuato in Mexico (Sep-2011 to Feb-2012). METHODS: Cross-sectional, observational study in 10 prisons in the State of Guanajuato in Mexico (2011-2012). We offered HCV-testing and applied audio computer-assisted self-interviews to all adults imprisoned in the State Prison System. We used a complex survey analysis to estimate the distribution of variables and its corresponding 95% confidence intervals, taking into consideration the expected cluster effect by common characteristics within prisons...
2017: PloS One
https://www.readbyqxmd.com/read/28654561/influenza-associated-encephalitis-encephalopathy-identified-by-the-australian-childhood-encephalitis-study-2013-2015
#13
Philip N Britton, Russell C Dale, Christopher C Blyth, Kristine Macartney, Nigel Crawford, Helen Marshall, Julia Clark, Elizabeth Elliott, Richard I Webster, Allen C Cheng, Robert Booy, Cheryl A Jones
BACKGROUND: Influenza associated encephalitis/encephalopathy (IAE) is an important cause of acute encephalitis syndrome in children. IAE includes a series of clinico-radiologic syndromes or acute encephalopathy syndromes that have been infrequently reported outside East Asia. We aimed to describe case of IAE identified by the Australian Childhood Encephalitis study. METHODS: Children ≤14 years of age with suspected encephalitis were prospectively identified in 5 hospitals in Australia...
June 24, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28654554/baseline-hepatitis-b-virus-titer-predicts-initial-postpartum-hepatic-flare-a-multicenter-prospective-study
#14
Jinfeng Liu, Jing Wang, Caijing Qi, Furong Cao, Zhen Tian, Dandan Guo, Taotao Yan, Qian Li, Shujuan Yang, JianJun Fu, Xianmei Tang, Xiaoni Kou, Na Liu, Zicheng Jiang, Yingren Zhao, Tianyan Chen
BACKGROUND AND GOALS: A series of changes in the immune system occur during pregnancy and puerperium. Currently, we aim to characterize both the natural changes in liver inflammation and its association with hepatitis B viremia during this special period. PATIENTS AND METHODS: Chronic hepatitis B (CHB) gravidas were recruited and followed up to 52 weeks postpartum. Virological and biochemical parameters were assessed throughout the period. RESULTS: A total of 1097 CHB mothers had finished the entire follow-up including 451 accepting telbivudine, 178 accepting tenofovir, and 468 without antiviral therapy...
June 26, 2017: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/28654374/severe-fever-with-thrombocytopenia-syndrome-virus-infection-in-minks-in-china
#15
Gui-Sheng Wang, Jin-Bao Wang, Fu-Lin Tian, Hua-Jie Zhang, Fei-Fei Yin, Cong Xu, Dong Xu, Yu-Ting Huang, Xue-Jie Yu
We analyzed the seroprevalence of tick-borne severe fever with thrombocytopenia syndrome virus (SFTSV) in farm-raised minks using double antigen ELISA (enzyme-linked immunosorbent assay) kit and indicated that 8.4% (15/178) of the minks had antibodies to the nucleoprotein of SFTSV and 72.7% (8/11) of mink farms had minks positive to SFTSV. The ELISA results were further confirmed by presence of neutralization to SFTSV in the mink sera. Our results suggested that minks were widely infected with SFTSV in China...
June 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28654319/partial-characterization-of-tick-borne-encephalitis-virus-isolates-from-ticks-of-southern-ukraine
#16
Oksana O Yurchenko, Dmytro O Dubina, Nataliya O Vynograd, Jean-Paul Gonzalez
Tick-borne encephalitis (TBE) is the most common tick-borne viral infection in Eurasia; thousands of human cases are annually reported from several European countries. Several tick species are vectors of the tick-borne encephalitis virus (TBEV), while TBE appears to be spreading from the Eurasian continent westward to Europe. Fifteen study sites were chosen from five territories of southern Ukraine, including Odessa, Mykolaiv, Kherson Oblast, the Autonomous Republic of Crimea, and Sevastopol. Tick collection was performed in spring season of three consecutive years (1988-1990) using either flagging technique or direct collection of specimens feeding on cattle...
June 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28654310/unique-infectious-strategy-of-h5n1-avian-influenza-virus-is-governed-by-the-acid-destabilized-property-of-hemagglutinin
#17
Tomo Daidoji, Yohei Watanabe, Yasuha Arai, Junichi Kajikawa, Ryohei Hirose, Takaaki Nakaya
Highly pathogenic avian influenza (HPAI) H5N1 virus emerged in 1997 as a zoonotic disease in Hong Kong. It has since spread to Asia and Europe and is a serious threat to both the poultry industry and human health. For effective surveillance and possible prevention/control of HPAI H5N1 viruses, it is necessary to understand the molecular mechanism underlying HPAI H5N1 pathogenesis. The hemagglutinin (HA) protein of influenza A viruses (IAVs) is one of the major determinants of host adaptation, transmissibility, and viral virulence...
June 27, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28654219/a-comparative-study-of-hepatitis-b-virus-x-protein-mutants-k130m-v131i-and-kv130-131mi-to-investigate-their-roles-in-fibrosis-cirrhosis-and-hepatocellular-carcinoma
#18
Zaheenul Islam Siddiqui, Sabihur Rahman Farooqui, Syed Ali Azam, Masarrat Afroz, Saima Wajid, Shama Parveen, Syed Naqui Kazim
Hepatitis B virus (HBV) genomic mutations A1762T, G1764A and AG1762/1764TA cause production of HBV X protein (HBx) mutants, namely K130M, V131I and KV130/131MI. These mutations are important biomarkers for the development of cirrhosis and hepatocellular carcinoma (HCC) in chronic HBV patients. The present study comparatively analyzes the impact of intracellular expression of HBx mutants on HCC cell line Huh7. It was found that expression of KV130/131MI induced: cell proliferation; altered expression of cell cycle regulatory genes in favor of cell proliferation; intracellular reactive oxygen species (ROS) production and mitochondrial depolarization...
June 27, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28654088/coherent-soft-x-ray-diffraction-imaging-of-coliphage-pr772-at-the-linac-coherent-light-source
#19
Hemanth K N Reddy, Chun Hong Yoon, Andrew Aquila, Salah Awel, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Sergey Bobkov, Maximilian Bucher, Gabriella A Carini, Sebastian Carron, Henry Chapman, Benedikt Daurer, Hasan DeMirci, Tomas Ekeberg, Petra Fromme, Janos Hajdu, Max Felix Hanke, Philip Hart, Brenda G Hogue, Ahmad Hosseinizadeh, Yoonhee Kim, Richard A Kirian, Ruslan P Kurta, Daniel S D Larsson, N Duane Loh, Filipe R N C Maia, Adrian P Mancuso, Kerstin Mühlig, Anna Munke, Daewoong Nam, Carl Nettelblad, Abbas Ourmazd, Max Rose, Peter Schwander, Marvin Seibert, Jonas A Sellberg, Changyong Song, John C H Spence, Martin Svenda, Gijs Van der Schot, Ivan A Vartanyants, Garth J Williams, P Lourdu Xavier
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus...
June 27, 2017: Scientific Data
https://www.readbyqxmd.com/read/28654076/a-method-to-test-the-efficacy-of-handwashing-for-the-removal-of-emerging-infectious-pathogens
#20
Marlene K Wolfe, Daniele S Lantagne
Handwashing is widely recommended to prevent infectious disease transmission. However, little comparable evidence exists on the efficacy of handwashing methods in general. Additionally, little evidence exists comparing handwashing methods to determine which are most efficacious at removing infectious pathogens. Research is needed to provide evidence for the different approaches to handwashing that may be employed during infectious disease outbreaks. Here, a laboratory method to assess the efficacy of handwashing methods at removing microorganisms from hands and their persistence in rinse water is described...
June 7, 2017: Journal of Visualized Experiments: JoVE
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