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https://www.readbyqxmd.com/read/28636666/molecular-and-microbiological-report-of-a-hospital-outbreak-of-ndm-1-carrying-enterobacteriaceae-in-mexico
#1
Paola Bocanegra-Ibarias, Elvira Garza-González, Rayo Morfín-Otero, Humberto Barrios, Licet Villarreal-Treviño, Eduardo Rodríguez-Noriega, Ulises Garza-Ramos, Santiago Petersen-Morfin, Jesus Silva-Sanchez
OBJECTIVES: To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS: From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, and plasmid profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28599753/communication-between-middle-ses-black-women-and-healthcare-providers-about-hiv-testing
#2
Niasha A Fray, Kia Lilly Caldwell
PURPOSE: This article explores the impact of patient and healthcare provider communication (PPC) on the HIV testing behaviors of middle socioeconomic status (SES) Black women in North Carolina. We explore how PPC about STIs and HIV (or the lack thereof) affects the provision of STI/HIV testing by either confirming the need for middle SES Black women to test routinely or potentially deterring women from feeling they need to be tested. METHODS/FINDINGS: After conducting 15 qualitative interviews with middle SES Black women between 25 and 45 years of age, we uncovered the role of patient self-advocacy in promoting HIV testing among middle SES Black women when they communicate with their healthcare providers...
July 2017: Journal of the National Medical Association
https://www.readbyqxmd.com/read/28590593/the-proteasome-enters-the-meiotic-prophase-fray
#3
REVIEW
Aleksandar Vujin, Monique Zetka
The segregation of homologous chromosomes in meiosis depends on their ability to locate one another in the nucleus and establish a physical association through crossing over. A tightly regulated number of crossovers (COs) emerges following repair of induced DNA double-strand breaks by homologous recombination (HR), but the process of how HR intermediates transition into COs is still poorly understood. Two recent studies by Ahuja et al. and Rao et al. have revealed a role for chromosomally localized proteasomes in choreographing both homologous chromosome pairing and the evolution of HR intermediates into segregation-competent COs...
June 7, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28554975/mechanical-and-electrostatic-experiments-with-dust-particles-collected-in-the-inner-coma-of-comet-67p-by-cosima-onboard-rosetta
#4
Martin Hilchenbach, Henning Fischer, Yves Langevin, Sihane Merouane, John Paquette, Jouni Rynö, Oliver Stenzel, Christelle Briois, Jochen Kissel, Andreas Koch, Rita Schulz, Johan Silen, Nicolas Altobelli, Donia Baklouti, Anais Bardyn, Herve Cottin, Cecile Engrand, Nicolas Fray, Gerhard Haerendel, Hartmut Henkel, Herwig Höfner, Klaus Hornung, Harry Lehto, Eva Maria Mellado, Paola Modica, Lena Le Roy, Sandra Siljeström, Wolfgang Steiger, Laurent Thirkell, Roger Thomas, Klaus Torkar, Kurt Varmuza, Boris Zaprudin
The in situ cometary dust particle instrument COSIMA (COmetary Secondary Ion Mass Analyser) onboard ESA's Rosetta mission has collected about 31 000 dust particles in the inner coma of comet 67P/Churyumov-Gerasimenko since August 2014. The particles are identified by optical microscope imaging and analysed by time-of-flight secondary ion mass spectrometry. After dust particle collection by low speed impact on metal targets, the collected particle morphology points towards four families of cometary dust particles...
July 13, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28551479/dna-and-protein-binding-double-strand-dna-cleavage-and-cytotoxicity-of-mixed-ligand-copper-ii-complexes-of-the-antibacterial-drug-nalidixic-acid
#5
Rangasamy Loganathan, Mani Ganeshpandian, Nattamai S P Bhuvanesh, Mallayan Palaniandavar, Amsaveni Muruganantham, Swapan K Ghosh, Anvarbatcha Riyasdeen, Mohammad Abdulkader Akbarsha
The water soluble mixed ligand complexes [Cu(nal)(diimine)(H2O)](ClO4) 1-4, where H(nal) is nalidixic acid and diimine is 2,2'-bipyridine (1), 1,10-phenanthroline (2), 5,6-dimethyl-1,10-phenanthroline (3), and 3,4,7,8-tetramethyl-1,10-phenanthroline (4), have been isolated. The coordination geometry around Cu(II) in 1 and that in the Density Functional Theory optimized structures of 1-4 has been assessed as square pyramidal. The trend in DNA binding constants (Kb) determined using absorption spectral titration (Kb: 1, 0...
May 3, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28545257/the-reactive-sulfur-species-concept-15-years-on
#6
REVIEW
Gregory I Giles, Muhammad Jawad Nasim, Wesam Ali, Claus Jacob
Fifteen years ago, in 2001, the concept of "Reactive Sulfur Species" or RSS was advocated as a working hypothesis. Since then various organic as well as inorganic RSS have attracted considerable interest and stimulated many new and often unexpected avenues in research and product development. During this time, it has become apparent that molecules with sulfur-containing functional groups are not just the passive "victims" of oxidative stress or simple conveyors of signals in cells, but can also be stressors in their own right, with pivotal roles in cellular function and homeostasis...
May 23, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28532839/dry-ice-is-a-reliable-substrate-for-the-distribution-of-frozen-mouse-spermatozoa-a-multi-centric-study
#7
Marcello Raspa, Mo Guan, Renata Paoletti, Lluis Montoliu, Abdel Ayadi, Susan Marschall, Martin Fray, Ferdinando Scavizzi
Disseminating mouse stocks as frozen materials offers both ethical and logistical advantages over live animal shipment, minimizing the welfare issues and avoiding some of the complex custom regulations that are associated with live animal transportation. Embryo freezing in liquid nitrogen (LN2) at -196 °C has traditionally been the method of choice for archiving mouse lines. However, spermatozoa freezing is emerging as a more convenient alternative due to the application of innovative cryopreservation and recovery protocols...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532080/antibiotic-loaded-microspheres-as-antimicrobial-delivery-systems-for-medical-applications
#8
J Szczeblinska, K Fijalkowski, J Kohn, M El Fray
In this paper, we present the preparation and antibiotic loading of polymeric microspheres, composed of copolymers derived from fatty acid/amino acid components, as new polymeric platforms for antibiotic delivery systems. New polymeric materials were used to prepare microspheres with and without immobilized model antibiotics (streptomycin, chloramphenicol and amphotericin B) by a W/O/W double-emulsion/solvent evaporation method, in which chloroform and poly(vinyl alcohol) are used as the solvent and emulsifier, respectively...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529661/slipped-capital-femoral-epiphysis-management-and-the-arthroscope
#9
F Accadbled, O May, C Thévenin-Lemoine, J Sales de Gauzy
BACKGROUND: In situ pinning of slipped capital femoral epiphysis (SCFE) results in various degrees of deformity of the femoral head-neck junction. Repetitive trauma from cam-type femoroacetabular impingement (FAI) can lead to labral tears and injury to the articular cartilage causing loss of function. Arthroscopic osteoplasty is an alternative to open procedure and to Southwick/Imhäuser-type osteotomies in symptomatic selected cases. SURGICAL TECHNIQUE: The amount of bone to be resected has to be carefully planned pre-operatively...
April 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28517978/the-effect-of-carbon-nanoparticles-on-biological-properties-of-polyester-nanocomposites
#10
Zygmunt Staniszewski, Agnieszka Piegat, Wieslawa Okroj, Magdalena Walkowiak-Przybylo, Witold Jakubowski, Bogdan Walkowiak, Boguslaw Budner, Waldemar Mroz, Peter Sobolewski, Miroslawa El Fray
The aim of present study was to determine the hemocompatibility, cellular response of endothelial cells and bacterial adhesion to a new polyester nanocomposite. The carbon nanoparticle nanocomposite was prepared via in situ polymerization of monomers to obtain material of hardness 55 Sh D similar to polyurethanes used in medical applications, for example, in heart-assisting devices. The carbon nanoparticle-containing polyester exhibits markedly reduced bacterial colonization, as compared to commercially available polyurethanes...
May 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28503769/identification-of-factors-required-for-m-6-a-mrna-methylation-in-arabidopsis-reveals-a-role-for-the-conserved-e3-ubiquitin-ligase-hakai
#11
Kamil Růžička, Mi Zhang, Ana Campilho, Zsuzsanna Bodi, Muhammad Kashif, Mária Saleh, Dominique Eeckhout, Sedeer El-Showk, Hongying Li, Silin Zhong, Geert De Jaeger, Nigel P Mongan, Jan Hejátko, Ykä Helariutta, Rupert G Fray
N6-adenosine methylation (m(6) A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m(6) A writer proteins in Arabidopsis thaliana. The components required for m(6) A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m(6) A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m(6) A methylation plays a role in developmental decisions during pattern formation...
July 2017: New Phytologist
https://www.readbyqxmd.com/read/28488445/highly-heterogeneous-nature-of-the-native-and-unfolded-states-of-the-b-domain-of-protein-a-revealed-by-two-dimensional-fluorescence-lifetime-correlation-spectroscopy
#12
Takuhiro Otosu, Kunihiko Ishii, Hiroyuki Oikawa, Munehito Arai, Satoshi Takahashi, Tahei Tahara
Elucidating the protein folding mechanism is crucial to understand how proteins acquire their unique structures to realize various biological functions. With this aim, the folding/unfolding of small globular proteins has been extensively studied. Interestingly, recent studies have revealed that even such small proteins represent considerably complex processes. In this study, we examined the folding/unfolding process of a small α-helical protein, the B domain of protein A (BdpA), at equilibrium using two-dimensional fluorescence lifetime correlation spectroscopy with 10 μs time resolution...
May 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28470843/combination-of-biochemical-and-mechanical-cues-for-tendon-tissue-engineering
#13
Stefano Testa, Marco Costantini, Ersilia Fornetti, Sergio Bernardini, Marcella Trombetta, Dror Seliktar, Stefano Cannata, Alberto Rainer, Cesare Gargioli
Tendinopathies negatively affect the life quality of millions of people in occupational and athletic settings, as well as the general population. Tendon healing is a slow process, often with insufficient results to restore complete endurance and functionality of the tissue. Tissue engineering, using tendon progenitors, artificial matrices and bioreactors for mechanical stimulation, could be an important approach for treating rips, fraying and tissue rupture. In our work, C3H10T1/2 murine fibroblast cell line was exposed to a combination of stimuli: a biochemical stimulus provided by Transforming Growth Factor Beta (TGF-β) and Ascorbic Acid (AA); a three-dimensional environment represented by PEGylated-Fibrinogen (PEG-Fibrinogen) biomimetic matrix; and a mechanical induction exploiting a custom bioreactor applying uniaxial stretching...
May 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28440765/association-of-metabolic-syndrome-with-low-birth-weight-intake-of-high-calorie-diets-and-acanthosis-nigricans-in-children-and-adolescents-with-overweight-and-obesity
#14
Marlen Velazquez-Bautista, Joana Jazmín López-Sandoval, Mercedes González-Hita, Eduardo Vázquez-Valls, Iris Zamihara Cabrera-Valencia, Blanca Miriam Torres-Mendoza
OBJECTIVE: The theory of fetal programming suggests that low birth weight (LBW) predisposes to greater food intake and increases the chance of overweight and obesity, which are in turn associated to conditions such as metabolic syndrome (MS) and acanthosis nigricans. The study objective was to ascertain whether an association exists between MS, LBW, intake of high-calorie diets, and acanthosis nigricans in children and adolescents with overweight or obesity. MATERIAL AND METHODS: A case-control was conducted on 100 children who attended the overweight and obesity outpatient clinic of the OPD Hospital Civil de Guadalajara "Fray Antonio Alcalde"...
January 2017: Endocrinología, diabetes y nutrición
https://www.readbyqxmd.com/read/28423273/conformational-dynamics-of-mechanically-compliant-dna-nanostructures-from-coarse-grained-molecular-dynamics-simulations
#15
Ze Shi, Carlos E Castro, Gaurav Arya
Structural DNA nanotechnology, the assembly of rigid 3D structures of complex yet precise geometries, has recently been used to design dynamic, mechanically compliant nanostructures with tunable equilibrium conformations and conformational distributions. Here we use coarse-grained molecular dynamics simulations to provide insights into the conformational dynamics of a set of mechanically compliant DNA nanostructures-DNA hinges that use single-stranded DNA "springs" to tune the equilibrium conformation of a layered double-stranded DNA "joint" connecting two stiff "arms" constructed from DNA helix bundles...
April 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28390594/tracheal-tear-from-blunt-neck-trauma-in-children-diagnosis-and-management
#16
David J Mener, Fray Dylan Stewart, David E Tunkel
We describe the management of posterior trachea tears after blunt neck trauma in two children. The first, a 5 year-old boy who fell off his scooter, causing a 1.0cm tear in the membranous cervical trachea, was managed conservatively with 5 days of intubation. The second, a 12 year-old girl who fell on her bicycle, causing a 4.0cm tear in the membranous thoracic trachea, was repaired with thoracoscopic techniques. The presumed mechanism may be expansion of the U-shaped cartilage with tear of the membranous trachea...
May 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28374492/in-vitro-laser-fenestration-of-aortic-stent-grafts-a-qualitative-analysis-under-scanning-electron-microscope
#17
Jing Lin, Naval Udgiri, Robert Guidoin, Jean Panneton, Xiaoning Guan, Maxime Guillemette, Lu Wang, Jia Du, Dajie Zhu, Mark Nutley, Ze Zhang
In situ fenestration of stent-grafts allows patients with life threatening aortic pathologies to be amenable to emergent "off the shelf indications for use" percutaneous treatments as a bail out technique. Three types of aortic stent-grafts were subjected to laser fenestration in a physiological saline solution followed by balloon angioplasty using 8, 10 or 12 mm in diameter noncompliant balloons. The morphology and the size of fenestrations were observed under optical and scanning electron microscopy. The damage to the fabrics was analyzed and quantified...
November 2016: Artificial Organs
https://www.readbyqxmd.com/read/28364033/case-report-acute-facial-swelling-in-a-recreational-technical-diver
#18
Peter Buzzacott, William K Dolen, James Chimiak
A recreational scuba diver wore a second scuba regulator against his face during a scuba dive, attached by an elastic rubber cord necklace. After surfacing, the diver's left face became swollen. Through a process of elimination all other items of scuba equipment were excluded as potential causes. A dive with the same equipment minus the necklace confirmed the involvement of the necklace in the pathogenesis of the hypersensitive reaction. In vitro ImmunoCap IgE assay was positive to latex (1.30 kUa/L), subsequent patch testing for contact dermatitis provoked a reaction for benzophenone-4, (a UV stabalizer) and Fourier Transform Infra Red spectroscopy identified the elastic as ethylene propylene rubber, containing additional unidentified compounds...
April 2017: Physiological Reports
https://www.readbyqxmd.com/read/28349455/detection-and-quantification-of-n-6-methyladenosine-in-messenger-rna-by-tlc
#19
Zsuzsanna Bodi, Rupert G Fray
The base-modified nucleotide, N (6)-methyladenosine, is a relatively abundant modification found in the mRNA of most higher eukaryotes. Methylation levels can change dependent upon environmental conditions, cell differentiation state, or following knockdown of members of the methylase complex, and it is often useful to directly measure and compare N (6)-methyladenosine levels between samples. Two dimensional chromatography of radiolabeled nucleotides, following specific nuclease treatments, provides a robust, sensitive, and reproducible assay for this modification...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28325701/evaluation-of-flexor-pollicis-longus-tendon-attrition-using-color-doppler-imaging-after-volar-plate-fixation-for-distal-radius-fracture
#20
Yoshitaka Tanaka, Hiroyuki Gotani, Koichi Yano, Kosuke Sasaki, Yoshitaka Hamada
PURPOSE: Early diagnosis of attritional wear of the flexor pollicis longus (FPL) tendon is essential in preventing subsequent tendon rupture. There are currently few objective methods of assessing FPL attrition. We hypothesized that color Doppler imaging could visibly detect FPL tendon attrition, and analyzed our results. METHODS: We evaluated ultrasound imaging of the contact between the FPL tendon and a volar locking plate using the real-time B-mode and Doppler waveforms of the FPL tendon using the continuous Doppler wave mode in 40 patients who underwent fixation of the distal volar locking plate for distal radius fracture...
March 18, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
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