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https://www.readbyqxmd.com/read/28842745/diagnosis-of-long-head-of-biceps-tendinopathy-in-rotator-cuff-tear-patients-correlation-of-imaging-and-arthroscopy-data
#1
Morgane Rol, Luc Favard, Julien Berhouet
INTRODUCTION: The goal of this prospective study was to assess the reliability of pre-operative cross-sectional imaging for the diagnosis of long head of biceps (LHB) tendinopathy in patients with a rotator cuff tear. METHODS: Cross-sectional imaging with MRI or CT arthrography data from 25 patients operated upon because of a rotator cuff tear between 1 October 2015 and 1 April 2016 was analysed by one experienced orthopaedic surgeon, one experienced radiologist and one orthopaedic resident...
August 25, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28754649/stretching-single-fibrin-fibers-hampers-their-lysis
#2
Wei Li, Tomas Lucioni, Rongzhong Li, Keith Bonin, Samuel S Cho, Martin Guthold
Blood clots, whose main structural component is a mesh of microscopic fibrin fibers, experience mechanical strain from blood flow, clot retraction and interactions with platelets and other cells. We developed a transparent, striated and highly stretchable substrate made from fugitive glue (a styrenic block copolymer) to investigate how mechanical strain affects lysis of single, suspended fibrin fibers. In this suspended fiber assay, lysis manifested itself by fiber elongation, thickening (disassembly), fraying and collapse...
July 25, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28712861/a-novel-golgi-cox-staining-method-for-detecting-and-characterizing-roles-of-the-hepatic-stellate-cells-in-liver-injury
#3
Ma de Jesús Gómez Villalobos, Susana Vidrio, Ruth Giles López, Gabriel Flores Gómez, Victoria Chagoya de Sánchez
The aim of this study was to investigate the utility of the Golgi-Cox method to characterize the distribution and morphological changes of the hepatic stellate cells (HSCs) in CCl4 liver damaged rats. Six-week-old male Wistar rats were injected with CCl4 for ten weeks. The livers were processed with the Golgi-Cox method, reticuline, and Massońs Trichrome stains, and analyzed under light microscopy. Histological evaluation of livers was made through the METAVIR score. In normal livers, the HSCs show stellate form with abundant thin cytoplasmic processes, distributed into hepatic lobule, mainly in zone 1...
June 30, 2017: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/28664247/association-between-leg-bowing-and-serum-alkaline-phosphatase-level-regardless-of-the-presence-of-a-radiographic-growth-plate-abnormality-in-pediatric-patients-with-genu-varum
#4
Yuko Sakamoto, Muneaki Ishijima, Mayuko Kinoshita, Lizu Liu, Mitsuyoshi Suzuki, Sung-Gon Kim, Koichi Kamata, Akifumi Tokita, Haruka Kaneko, Toshiaki Shimizu, Kazuo Kaneko, Masahiko Nozawa
When children around 2 years of age show leg bowing and diseases are ruled out based on radiographic findings without conducting blood tests, they are classified as "physiologic" genu varum. Since whether or not physiologic genu varum is associated with bone metabolism is unclear, this study was conducted to clarify the association between genu varum and bone metabolism in children. Thirty-five pediatric patients with genu varm who visited our out-patient clinic were enrolled. While two of the 35 children had nutritional rickets, showing abnormalities on both blood test (ALP, ≥1000 IU/L; iPTH, >65 pg/mL and 25(OH)D, ≤20 ng/mL) and radiographs (such as cupping, fraying or splaying), five of 35 children showed abnormalities on blood tests but not radiographs...
June 29, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/28636666/molecular-and-microbiological-report-of-a-hospital-outbreak-of-ndm-1-carrying-enterobacteriaceae-in-mexico
#5
Paola Bocanegra-Ibarias, Elvira Garza-González, Rayo Morfín-Otero, Humberto Barrios, Licet Villarreal-Treviño, Eduardo Rodríguez-Noriega, Ulises Garza-Ramos, Santiago Petersen-Morfin, Jesus Silva-Sanchez
OBJECTIVES: To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS: From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, and plasmid profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28551479/dna-and-protein-binding-double-strand-dna-cleavage-and-cytotoxicity-of-mixed-ligand-copper-ii-complexes-of-the-antibacterial-drug-nalidixic-acid
#6
Rangasamy Loganathan, Mani Ganeshpandian, Nattamai S P Bhuvanesh, Mallayan Palaniandavar, Amsaveni Muruganantham, Swapan K Ghosh, Anvarbatcha Riyasdeen, Mohammad Abdulkader Akbarsha
The water soluble mixed ligand complexes [Cu(nal)(diimine)(H2O)](ClO4) 1-4, where H(nal) is nalidixic acid and diimine is 2,2'-bipyridine (1), 1,10-phenanthroline (2), 5,6-dimethyl-1,10-phenanthroline (3), and 3,4,7,8-tetramethyl-1,10-phenanthroline (4), have been isolated. The coordination geometry around Cu(II) in 1 and that in the Density Functional Theory optimized structures of 1-4 has been assessed as square pyramidal. The trend in DNA binding constants (Kb) determined using absorption spectral titration (Kb: 1, 0...
September 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28440765/association-of-metabolic-syndrome-with-low-birth-weight-intake-of-high-calorie-diets-and-acanthosis-nigricans-in-children-and-adolescents-with-overweight-and-obesity
#7
Marlen Velazquez-Bautista, Joana Jazmín López-Sandoval, Mercedes González-Hita, Eduardo Vázquez-Valls, Iris Zamihara Cabrera-Valencia, Blanca Miriam Torres-Mendoza
OBJECTIVE: The theory of fetal programming suggests that low birth weight (LBW) predisposes to greater food intake and increases the chance of overweight and obesity, which are in turn associated to conditions such as metabolic syndrome (MS) and acanthosis nigricans. The study objective was to ascertain whether an association exists between MS, LBW, intake of high-calorie diets, and acanthosis nigricans in children and adolescents with overweight or obesity. MATERIAL AND METHODS: A case-control was conducted on 100 children who attended the overweight and obesity outpatient clinic of the OPD Hospital Civil de Guadalajara "Fray Antonio Alcalde"...
January 2017: Endocrinología, diabetes y nutrición
https://www.readbyqxmd.com/read/28390594/tracheal-tear-from-blunt-neck-trauma-in-children-diagnosis-and-management
#8
David J Mener, Fray Dylan Stewart, David E Tunkel
We describe the management of posterior trachea tears after blunt neck trauma in two children. The first, a 5 year-old boy who fell off his scooter, causing a 1.0cm tear in the membranous cervical trachea, was managed conservatively with 5 days of intubation. The second, a 12 year-old girl who fell on her bicycle, causing a 4.0cm tear in the membranous thoracic trachea, was repaired with thoracoscopic techniques. The presumed mechanism may be expansion of the U-shaped cartilage with tear of the membranous trachea...
May 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28364033/case-report-acute-facial-swelling-in-a-recreational-technical-diver
#9
Peter Buzzacott, William K Dolen, James Chimiak
A recreational scuba diver wore a second scuba regulator against his face during a scuba dive, attached by an elastic rubber cord necklace. After surfacing, the diver's left face became swollen. Through a process of elimination all other items of scuba equipment were excluded as potential causes. A dive with the same equipment minus the necklace confirmed the involvement of the necklace in the pathogenesis of the hypersensitive reaction. In vitro ImmunoCap IgE assay was positive to latex (1.30 kUa/L), subsequent patch testing for contact dermatitis provoked a reaction for benzophenone-4, (a UV stabalizer) and Fourier Transform Infra Red spectroscopy identified the elastic as ethylene propylene rubber, containing additional unidentified compounds...
April 2017: Physiological Reports
https://www.readbyqxmd.com/read/28325701/evaluation-of-flexor-pollicis-longus-tendon-attrition-using-color-doppler-imaging-after-volar-plate-fixation-for-distal-radius-fracture
#10
Yoshitaka Tanaka, Hiroyuki Gotani, Koichi Yano, Kosuke Sasaki, Yoshitaka Hamada
PURPOSE: Early diagnosis of attritional wear of the flexor pollicis longus (FPL) tendon is essential in preventing subsequent tendon rupture. There are currently few objective methods of assessing FPL attrition. We hypothesized that color Doppler imaging could visibly detect FPL tendon attrition, and analyzed our results. METHODS: We evaluated ultrasound imaging of the contact between the FPL tendon and a volar locking plate using the real-time B-mode and Doppler waveforms of the FPL tendon using the continuous Doppler wave mode in 40 patients who underwent fixation of the distal volar locking plate for distal radius fracture...
March 18, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28139008/oxidative-stress-mediated-developmental-toxicity-induced-by-isoniazide-in-zebrafish-embryos-and-larvae
#11
Yu Zou, Yun Zhang, Liwen Han, Qiuxia He, Hairong Hou, Jian Han, Ximin Wang, Chengyun Li, Juan Cen, Kechun Liu
Isoniazide (INH) is an important first-line drug that is used to treat tuberculosis. However, the effect of INH on fetal growth has not yet been elucidated, and the mechanism of INH-induced developmental toxicity is still unknown. In the present study, we employed zebrafish embryos and larvae to investigate the developmental toxicity of INH. The survival rates of the embryos and larvae as well as the hatching rates of embryos were significantly reduced. Morphological abnormalities, including spinal curvature, yolk retention, swimming bladder absence, tail bending and shorter body lengths were induced by INH...
January 31, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28132766/piloting-a-pediatric-trauma-course-in-western-jamaica-lessons-learned-and-future-directions
#12
Hadley K Wesson, Valerie Plant, Marieka Helou, Karen Wharton, Delroy Fray, Jeffrey Haynes, Charles Bagwell
INTRODUCTION: Pediatric injuries are a leading cause of death in low- and middle-income countries (LMICs). Despite this, there are few formal pediatric-specific trauma educational initiatives available in LMICs. While new educational tools are being developed to address this, they have not been piloted in LMICs. In Jamaica, pediatric injuries are a leading cause of hospital admission but care is limited by a lack of training in triage and stabilization. Our objective was to implement and evaluate a pediatric trauma course in Jamaica to determine the impact this may have on further course development...
July 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27982106/protein-knotting-through-concatenation-significantly-reduces-folding-stability
#13
Shang-Te Danny Hsu
Concatenation by covalent linkage of two protomers of an intertwined all-helical HP0242 homodimer from Helicobacter pylori results in the first example of an engineered knotted protein. While concatenation does not affect the native structure according to X-ray crystallography, the folding kinetics is substantially slower compared to the parent homodimer. Using NMR hydrogen-deuterium exchange analysis, we showed here that concatenation destabilises significantly the knotted structure in solution, with some regions close to the covalent linkage being destabilised by as much as 5 kcal mol(-1)...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27919081/m-6-a-potentiates-sxl-alternative-pre-mrna-splicing-for-robust-drosophila-sex-determination
#14
Irmgard U Haussmann, Zsuzsanna Bodi, Eugenio Sanchez-Moran, Nigel P Mongan, Nathan Archer, Rupert G Fray, Matthias Soller
N(6)-methyladenosine (m(6)A) is the most common internal modification of eukaryotic messenger RNA (mRNA) and is decoded by YTH domain proteins. The mammalian mRNA m(6)A methylosome is a complex of nuclear proteins that includes METTL3 (methyltransferase-like 3), METTL14, WTAP (Wilms tumour 1-associated protein) and KIAA1429. Drosophila has corresponding homologues named Ime4 and KAR4 (Inducer of meiosis 4 and Karyogamy protein 4), and Female-lethal (2)d (Fl(2)d) and Virilizer (Vir). In Drosophila, fl(2)d and vir are required for sex-dependent regulation of alternative splicing of the sex determination factor Sex lethal (Sxl)...
December 8, 2016: Nature
https://www.readbyqxmd.com/read/27911771/stability-folding-dynamics-and-long-range-conformational-transition-of-the-synaptic-t-snare-complex
#15
Xinming Zhang, Aleksander A Rebane, Lu Ma, Feng Li, Junyi Jiao, Hong Qu, Frederic Pincet, James E Rothman, Yongli Zhang
Synaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) couple their stepwise folding to fusion of synaptic vesicles with plasma membranes. In this process, three SNAREs assemble into a stable four-helix bundle. Arguably, the first and rate-limiting step of SNARE assembly is the formation of an activated binary target (t)-SNARE complex on the target plasma membrane, which then zippers with the vesicle (v)-SNARE on the vesicle to drive membrane fusion. However, the t-SNARE complex readily misfolds, and its structure, stability, and dynamics are elusive...
December 13, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27864948/using-frons-width-to-differentiate-blow-fly-species-diptera-calliphoridae-phormia-regina-meigen-and-protophormia-terraenovae-robineau-desvoidy
#16
Sarah V Langer, Christopher J Kyle, David V Beresford
Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae) and Phormia regina (Meigen) (Diptera: Calliphoridae) are morphologically similar blow fly species commonly used for estimating postmortem intervals. Field collection and storage of adults can result in color changes, in particular on calypters and palps; often collected specimens show damage such as wing fray or fungal growth. We measured the frons width: total head width ratio using photographs (ImageJ version 1.49) to differentiate these two species...
November 16, 2016: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27596559/individuals-motivated-to-participate-in-adherence-care-and-treatment-impact-development-of-a-multi-component-intervention-to-help-hiv-infected-recently-incarcerated-individuals-link-and-adhere-to-hiv-care
#17
RANDOMIZED CONTROLLED TRIAL
Carol E Golin, Kevin Knight, Jessica Carda-Auten, Michele Gould, Jennifer Groves, Becky L White, Steve Bradley-Bull, Kemi Amola, Niasha Fray, David L Rosen, Michael J Mugavaro, Brian W Pence, Patrick M Flynn, David Wohl
BACKGROUND: Policy-makers promote a seek, test, treat and retain (STTR) strategy to expand HIV testing, support linkage and engagement in care, and enhance the continuous use of antiretroviral therapy for those HIV-infected. This HIV prevention strategy is particularly appropriate in correctional settings where HIV screening and treatment are routinely available yet many HIV-infected individuals have difficulty sustaining sufficient linkage and engagement in care, disease management, and viral suppression after prison release...
September 6, 2016: BMC Public Health
https://www.readbyqxmd.com/read/27564136/contact-and-support-considerations-in-the-hydrogen-evolution-reaction-activity-of-petaled-mos2-electrodes
#18
Shane T Finn, Janet E Macdonald
Petaled MoS2 electrodes grown hydrothermally from Mo foils are found to have an 800 nm, intermediate, MoSxOy layer. Similar petaled MoS2 films without this intermediate layer are grown on Au. X-ray photoelectron and Raman spectroscopies and transmission electron microscopy indicate the resulting petaled multilayer MoS2 films are frayed and exhibit single-layer, 1T-MoS2 behavior at the edges. We compare the electrocatalytic hydrogen evolution reaction activity via linear sweep voltammetry with Tafel analysis as well as the impedance properties of the electrodes...
September 28, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27535119/cytologic-findings-following-intraoperative-use-of-floseal-r-hemostatic-matrix-a-potential-diagnostic-pitfall-in-fluid-aspiration-cytology
#19
Julieta E Barroeta, Elliott Lhospital, Eugene Smith, Emily M Ko
We report the case of a 62-year-old woman who underwent laparoscopic hysterectomy, bilateral salpingo-oophorectomy, and lymph node dissection for endometrial endometrioid carcinoma FIGO grade 1. Cytologic examination of the pelvic washing specimen showed an acellular matrix with frayed edges and cracking artifact. Upon investigation, it became apparent that this could represent hemostatic matrix Floseal(R) that had been used intraoperatively at the lymphadenectomy site. Laboratory replication of the cytologic findings with a Floseal(R) sample confirmed it...
December 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/27513582/insights-into-the-conformation-of-the-membrane-proximal-regions-critical-to-the-trimerization-of-the-hiv-1-gp41-ectodomain-bound-to-dodecyl-phosphocholine-micelles
#20
John M Louis, James L Baber, Rodolfo Ghirlando, Annie Aniana, Ad Bax, Julien Roche
The transitioning of the ectodomain of gp41 from a pre-hairpin to a six-helix bundle conformation is a crucial aspect of virus-cell fusion. To gain insight into the intermediary steps of the fusion process we have studied the pH and dodecyl phosphocholine (DPC) micelle dependent trimer association of gp41 by systematic deletion analysis of an optimized construct termed 17-172 (residues 528 to 683 of Env) that spans the fusion peptide proximal region (FPPR) to the membrane proximal external region (MPER) of gp41, by sedimentation velocity and double electron-electron resonance (DEER) EPR spectroscopy...
2016: PloS One
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