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https://www.readbyqxmd.com/read/27982106/protein-knotting-through-concatenation-significantly-reduces-folding-stability
#1
Shang-Te Danny Hsu
Concatenation by covalent linkage of two protomers of an intertwined all-helical HP0242 homodimer from Helicobacter pylori results in the first example of an engineered knotted protein. While concatenation does not affect the native structure according to X-ray crystallography, the folding kinetics is substantially slower compared to the parent homodimer. Using NMR hydrogen-deuterium exchange analysis, we showed here that concatenation destabilises significantly the knotted structure in solution, with some regions close to the covalent linkage being destabilised by as much as 5 kcal mol(-1)...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27919081/m-6-a-potentiates-sxl-alternative-pre-mrna-splicing-for-robust-drosophila-sex-determination
#2
Irmgard U Haussmann, Zsuzsanna Bodi, Eugenio Sanchez-Moran, Nigel P Mongan, Nathan Archer, Rupert G Fray, Matthias Soller
N(6)-methyladenosine (m(6)A) is the most common internal modification of eukaryotic messenger RNA (mRNA) and is decoded by YTH domain proteins. The mammalian mRNA m(6)A methylosome is a complex of nuclear proteins that includes METTL3 (methyltransferase-like 3), METTL14, WTAP (Wilms tumour 1-associated protein) and KIAA1429. Drosophila has corresponding homologues named Ime4 and KAR4 (Inducer of meiosis 4 and Karyogamy protein 4), and Female-lethal (2)d (Fl(2)d) and Virilizer (Vir). In Drosophila, fl(2)d and vir are required for sex-dependent regulation of alternative splicing of the sex determination factor Sex lethal (Sxl)...
December 8, 2016: Nature
https://www.readbyqxmd.com/read/27911771/stability-folding-dynamics-and-long-range-conformational-transition-of-the-synaptic-t-snare-complex
#3
Xinming Zhang, Aleksander A Rebane, Lu Ma, Feng Li, Junyi Jiao, Hong Qu, Frederic Pincet, James E Rothman, Yongli Zhang
Synaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) couple their stepwise folding to fusion of synaptic vesicles with plasma membranes. In this process, three SNAREs assemble into a stable four-helix bundle. Arguably, the first and rate-limiting step of SNARE assembly is the formation of an activated binary target (t)-SNARE complex on the target plasma membrane, which then zippers with the vesicle (v)-SNARE on the vesicle to drive membrane fusion. However, the t-SNARE complex readily misfolds, and its structure, stability, and dynamics are elusive...
December 13, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27864948/using-frons-width-to-differentiate-blow-fly-species-diptera-calliphoridae-phormia-regina-meigen-and-protophormia-terraenovae-robineau-desvoidy
#4
Sarah V Langer, Christopher J Kyle, David V Beresford
Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae) and Phormia regina (Meigen) (Diptera: Calliphoridae) are morphologically similar blow fly species commonly used for estimating postmortem intervals. Field collection and storage of adults can result in color changes, in particular on calypters and palps; often collected specimens show damage such as wing fray or fungal growth. We measured the frons width: total head width ratio using photographs (ImageJ version 1.49) to differentiate these two species...
November 16, 2016: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27596559/individuals-motivated-to-participate-in-adherence-care-and-treatment-impact-development-of-a-multi-component-intervention-to-help-hiv-infected-recently-incarcerated-individuals-link-and-adhere-to-hiv-care
#5
Carol E Golin, Kevin Knight, Jessica Carda-Auten, Michele Gould, Jennifer Groves, Becky L White, Steve Bradley-Bull, Kemi Amola, Niasha Fray, David L Rosen, Michael J Mugavaro, Brian W Pence, Patrick M Flynn, David Wohl
BACKGROUND: Policy-makers promote a seek, test, treat and retain (STTR) strategy to expand HIV testing, support linkage and engagement in care, and enhance the continuous use of antiretroviral therapy for those HIV-infected. This HIV prevention strategy is particularly appropriate in correctional settings where HIV screening and treatment are routinely available yet many HIV-infected individuals have difficulty sustaining sufficient linkage and engagement in care, disease management, and viral suppression after prison release...
September 6, 2016: BMC Public Health
https://www.readbyqxmd.com/read/27564136/contact-and-support-considerations-in-the-hydrogen-evolution-reaction-activity-of-petaled-mos2-electrodes
#6
Shane T Finn, Janet E Macdonald
Petaled MoS2 electrodes grown hydrothermally from Mo foils are found to have an 800 nm, intermediate, MoSxOy layer. Similar petaled MoS2 films without this intermediate layer are grown on Au. X-ray photoelectron and Raman spectroscopies and transmission electron microscopy indicate the resulting petaled multilayer MoS2 films are frayed and exhibit single-layer, 1T-MoS2 behavior at the edges. We compare the electrocatalytic hydrogen evolution reaction activity via linear sweep voltammetry with Tafel analysis as well as the impedance properties of the electrodes...
September 28, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27535119/cytologic-findings-following-intraoperative-use-of-floseal-r-hemostatic-matrix-a-potential-diagnostic-pitfall-in-fluid-aspiration-cytology
#7
Julieta E Barroeta, Elliott Lhospital, Eugene Smith, Emily M Ko
We report the case of a 62-year-old woman who underwent laparoscopic hysterectomy, bilateral salpingo-oophorectomy, and lymph node dissection for endometrial endometrioid carcinoma FIGO grade 1. Cytologic examination of the pelvic washing specimen showed an acellular matrix with frayed edges and cracking artifact. Upon investigation, it became apparent that this could represent hemostatic matrix Floseal(R) that had been used intraoperatively at the lymphadenectomy site. Laboratory replication of the cytologic findings with a Floseal(R) sample confirmed it...
December 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/27513582/insights-into-the-conformation-of-the-membrane-proximal-regions-critical-to-the-trimerization-of-the-hiv-1-gp41-ectodomain-bound-to-dodecyl-phosphocholine-micelles
#8
John M Louis, James L Baber, Rodolfo Ghirlando, Annie Aniana, Ad Bax, Julien Roche
The transitioning of the ectodomain of gp41 from a pre-hairpin to a six-helix bundle conformation is a crucial aspect of virus-cell fusion. To gain insight into the intermediary steps of the fusion process we have studied the pH and dodecyl phosphocholine (DPC) micelle dependent trimer association of gp41 by systematic deletion analysis of an optimized construct termed 17-172 (residues 528 to 683 of Env) that spans the fusion peptide proximal region (FPPR) to the membrane proximal external region (MPER) of gp41, by sedimentation velocity and double electron-electron resonance (DEER) EPR spectroscopy...
2016: PloS One
https://www.readbyqxmd.com/read/27507063/diagnostic-utility-of-arborizing-stromal-meshwork-fragments-in-mucinous-tumors
#9
Stell Patadji, Joshua Pantanowitz, Natalie M Perlov, Sara E Monaco
INTRODUCTION: Arborizing stromal meshwork fragments (ASMFs) have been proposed as a useful clue to differentiate mucin associated with mucinous adenocarcinoma from contaminating mucus during endobronchial ultrasound-guided transbronchial needle aspiration. Our aim was to retrospectively review cytology cases with mucinous material to determine the utility of ASMFs in diagnosing mucinous tumors. MATERIAL AND METHODS: Diff-Quik stained smears from archival cytology cases (N = 40) were reviewed, including adenocarcinomas with mucinous features, cystic mucinous neoplasms, and control cases with mucin contamination...
October 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/27496052/aneuploidy-screening-of-embryonic-stem-cell-clones-by-metaphase-karyotyping-and-droplet-digital-polymerase-chain-reaction
#10
Gemma F Codner, Loic Lindner, Adam Caulder, Marie Wattenhofer-Donzé, Adam Radage, Annelyse Mertz, Benjamin Eisenmann, Joffrey Mianné, Edward P Evans, Colin V Beechey, Martin D Fray, Marie-Christine Birling, Yann Hérault, Guillaume Pavlovic, Lydia Teboul
BACKGROUND: Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromosomes. RESULTS: As part of the International Mouse Phenotyping Consortium, we gathered data from over 1,500 ES cell clones and found that the germline transmission (GLT) efficiency of clones is compromised when over 50 % of cells harbour chromosome number abnormalities...
2016: BMC Cell Biology
https://www.readbyqxmd.com/read/27417132/survival-following-laparoscopic-adrenalectomy-for-solitary-metastasis-of-lung-cancer
#11
Senol Tonyali, Hakan Bahadir Haberal, Sertac Yazici, Mustafa Erman, Zafer Volkan Kaynaroglu, Cenk Yucel Bilen
PURPOSE: To determine if L/S ADX is a safe and effective procedure for the management of solitary adrenal metastasis of lung cancer, and if it improves survival in lung cancer patients. MATERIALS AND METHODS: The medical records of patients that were diagnosed as lung cancer and underwent L/S ADX for suspected adrenal gland metastasis were retrospectively analyzed. Patient demographics, prior interventions and medical treatments for lung cancer, adrenal mass characteristics, surgical features, postoperative course, and histopathological findings were examined...
November 2016: International Urology and Nephrology
https://www.readbyqxmd.com/read/27376238/genome-wide-association-of-multiple-complex-traits-in-outbred-mice-by-ultra-low-coverage-sequencing
#12
Jérôme Nicod, Robert W Davies, Na Cai, Carl Hassett, Leo Goodstadt, Cormac Cosgrove, Benjamin K Yee, Vikte Lionikaite, Rebecca E McIntyre, Carol Ann Remme, Elisabeth M Lodder, Jennifer S Gregory, Tertius Hough, Russell Joynson, Hayley Phelps, Barbara Nell, Clare Rowe, Joe Wood, Alison Walling, Nasrin Bopp, Amarjit Bhomra, Polinka Hernandez-Pliego, Jacques Callebert, Richard M Aspden, Nick P Talbot, Peter A Robbins, Mark Harrison, Martin Fray, Jean-Marie Launay, Yigal M Pinto, David A Blizard, Connie R Bezzina, David J Adams, Paul Franken, Tom Weaver, Sara Wells, Steve D M Brown, Paul K Potter, Paul Klenerman, Arimantas Lionikas, Richard Mott, Jonathan Flint
Two bottlenecks impeding the genetic analysis of complex traits in rodents are access to mapping populations able to deliver gene-level mapping resolution and the need for population-specific genotyping arrays and haplotype reference panels. Here we combine low-coverage (0.15×) sequencing with a new method to impute the ancestral haplotype space in 1,887 commercially available outbred mice. We mapped 156 unique quantitative trait loci for 92 phenotypes at a 5% false discovery rate. Gene-level mapping resolution was achieved at about one-fifth of the loci, implicating Unc13c and Pgc1a at loci for the quality of sleep, Adarb2 for home cage activity, Rtkn2 for intensity of reaction to startle, Bmp2 for wound healing, Il15 and Id2 for several T cell measures and Prkca for bone mineral content...
August 2016: Nature Genetics
https://www.readbyqxmd.com/read/27373179/editorial-commentary-ligamentum-teres-tears-and-femoroacetabular-impingement-complex-coexistence-of-impingement-and-instability
#13
EDITORIAL
Christopher M Larson
In a large Level IV case series of 2,213 hip arthroscopies with the diagnosis of femoroacetabular impingement, the intraoperative status of the ligamentum teres (LT) was recorded as normal in 11%, frayed and/or partially torn in 88%, and completely torn in 1.5% of hips. Although specific physical examination maneuvers for detecting LT tears were not available early in the study period, thus limiting a detailed assessment of such tests, the authors identified that female gender, a lower lateral center edge angle, a higher Tonnis angle, and capsular laxity were all associated with complete LT tears...
July 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27353739/the-first-case-of-vitamin-d-dependent-rickets-type-1a-from-uzbek-population-with-a-novel-mutation
#14
Bahar Özcabı, Feride Tahmiscioğlu Bucak, Sevinç Jaferova, Çiğdem Oruç, Amra Adrovic, Serdar Ceylaner, Oya Ercan, Olcay Evliyaoğlu
BACKGROUND: Vitamin D dependent rickets type 1A (VDDR-1A) (OMIM#264700) is a rare, autosomal-recessively inherited disorder due to inactivating mutations in CYP27B1. It is characterized by early onset of rickets with hypocalcemia. We aimed to describe clinical, laboratory findings in VDDR-1A and report a novel homozygote truncating mutation NM_000785.3 c.403C>T (p.Q135*) in CYP27B1 which as far as we know is the first described mutation in Uzbek population. CASE: The patient was admitted with tetany at the age of 12 months...
June 29, 2016: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/27234143/incidental-injury-and-repair-of-obturator-nerve-during-laparoscopic-pelvic-lymphadenectomy
#15
Gulden Menderes, Nicole Vilardo, Carlton L Schwab, Masoud Azodi
OBJECTIVE: To demonstrate a surgical video wherein left obturator nerve was iatrogenically injured during pelvic lymphadenectomy and repaired immediately with laparoscopic epineural end-to-end tension free anastomosis. METHODS: This is a step-by-step demonstration of an incidental injury and laparoscopic repair of left obturator nerve during pelvic lymphadenectomy. The patient was a 59year-old Hispanic female who was found to have endometrial adenocarcinoma. She was referred to our division for laparoscopic staging during which left obturator nerve was iatrogenically injured...
July 2016: Gynecologic Oncology
https://www.readbyqxmd.com/read/27231211/towards-large-scale-preparation-of-graphene-in-molten-salts-and-its-use-in-the-fabrication-of-highly-toughened-alumina-ceramics
#16
Ali Reza Kamali, John Feighan, Derek J Fray
Highly crystalline graphene nanosheets were reproducibly generated by the electrochemical exfoliation of graphite electrodes in molten LiCl containing protons. The graphene product has been successfully applied in several applications. This paper discusses the effect of molten salt produced graphene on the microstructures and mechanical properties of alumina articles produced by slip casting and pressureless sintering, which is one of the most convenient methods for the commercial production of alumina ceramics...
August 15, 2016: Faraday Discussions
https://www.readbyqxmd.com/read/27183859/clinical-outcomes-and-material-properties-of-in-situ-fenestration-of-endovascular-stent-grafts
#17
REVIEW
Sean A Crawford, Ryan M Sanford, Thomas L Forbes, Cristina H Amon, Matthew G Doyle
OBJECTIVE: In situ fenestration of endovascular stent grafts has been used as a method for branch vessel revascularization in urgent and emergent settings. The objective of this manuscript was to review the clinical and experimental evidence related to this technique. METHODS: PubMed, MEDLINE, and Embase databases were searched for papers published until December 2015 describing in situ fenestration of aortic stent grafts. Benchtop, animal, and human studies were included...
July 2016: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/27100976/-frailty-in-the-elderly-and-nutritional-status-according-to-the-mini-nutritional-assessment
#18
Nadia Belén Guerrero-García, Zoila Margarita Gómez-Lomelí, David Leal-Mora, Oscar Loreto-Garibay
BACKGROUND: The aging process involves psychosocial and physiological changes, which modify the nutritional status in the elderly. The objective was to assess the nutritional status of a group of elderly patients using the Mini Nutritional Assessment (MNA) and the Fried frailty index, as well as the relation between both. METHODS: From June to July, 2013, we carried out a cross-sectional study with consecutive sampling of 146 patients of both sexes, from the Hospital Civil "Fray Antonio Alcalde", in Guadalajara, México...
May 2016: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/27085306/pathological-damage-and-immunomodulatory-effects-of-zebrafish-exposed-to-microcystin-lr
#19
Chuanyue Chen, Wanjing Liu, Li Wang, Jian Li, Yuanyuan Chen, Jienan Jin, Atufa Kawan, Xuezhen Zhang
Cyanobacterial blooms caused by water eutrophication have become a worldwide problem. Microcystins (MCs), especially microcystin-LR (MC-LR), released during cyanobacterial blooms exert great toxicity on fish and even lead to massive death. The present study mainly investigated the pathological damage and immune response of spleen, gut and gill in zebrafish exposed to MC-LR. Fish were exposed to 0, 1, 5 and 20 μg/L of MC-LR for 30 d. In zebrafish exposed to 5 and 20 μg/L MC-LR, edematous mitochondria, deformation of the nucleus and compaction of chromatin were observed in lymphocyte of spleen; frayed gut villi, exfoliation of epithelial cells and widespread cell lyses were observed in intestines; hyperemia in gill lamellae, epithelial tissue edema and uplift and lamellar fusion were observed in gill...
August 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27083534/ligamentum-teres-tears-and-femoroacetabular-impingement-prevalence-and-preoperative-findings
#20
Jorge Chahla, Eduardo A M Soares, Brian M Devitt, Lourenço P Peixoto, Peter Goljan, Karen K Briggs, Marc J Philippon
PURPOSE: To determine prevalence of ligamentum teres (LT) injuries identified during hip arthroscopy for treatment of femoroacetabular impingement (FAI) and to identify physical examination findings or radiographic characteristics specific to patients with complete LT tears that could possibly assist in a preoperative diagnosis. METHODS: Between October 2006 and June 2015, prospective data were collected on consecutive hip arthroscopies. Inclusion criterion was primary hip arthroscopy in patients aged 18 years or older...
July 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
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