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https://www.readbyqxmd.com/read/28730081/effect-of-human-autologous-serum-and-fetal-bovine-serum-on-human-corneal-epithelial-cell-viability-migration-and-proliferation-in-vitro
#1
Ming-Feng Wu, Tanja Stachon, Berthold Seitz, Achim Langenbucher, Nóra Szentmáry
AIM: To analyze the concentration-dependent effects of autologous serum (AS) and fetal bovine serum (FBS) on human corneal epithelial cell (HCEC) viability, migration and proliferation. METHODS: AS was prepared from 13 patients with non-healing epithelial defects Dulbecco's modified eagle medium/Ham's F12 (DMEM/F12) with 5% FBS, 0.5% dimethyl sulphoxide (DMSO), 10 ng/mL human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28715585/retinoic-acid-engineered-amniotic-membrane-used-as-graft-or-homogenate-positive-effects-on-corneal-alkali-burns
#2
Romain Joubert, Estelle Daniel, Nicolas Bonnin, Aurélie Comptour, Christelle Gross, Corinne Belville, Frédéric Chiambaretta, Loïc Blanchon, Vincent Sapin
Purpose: Alkali burns are the most common, severe chemical ocular injuries, their functional prognosis depending on corneal wound healing efficiency. The purpose of our study was to compare the benefits of amniotic membrane (AM) grafts and homogenates for wound healing in the presence or absence of previous all-trans retinoic acid (atRA) treatment. Methods: Fifty male CD1 mice with reproducible corneal chemical burn were divided into five groups, as follows: group 1 was treated with saline solution; groups 2 and 3 received untreated AM grafts or grafts treated with atRA, respectively; and groups 4 and 5 received untreated AM homogenates or homogenates treated with atRA, respectively...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28708898/retreatment-strategies-following-small-incision-lenticule-extraction-smile-in-vivo-tissue-responses
#3
Andri K Riau, Yu-Chi Liu, Chris H L Lim, Nyein C Lwin, Ericia P Teo, Gary H Yam, Donald T Tan, Jodhbir S Mehta
With any refractive correction, including Small Incision Lenticule Extraction (SMILE), there may be a residual refractive error that requires a retreatment. Here, we investigated the tissue responses following various retreatment procedures in a rabbit model of SMILE. All rabbits underwent a -6.00D correction with SMILE. Two weeks later, they underwent -1.00D enhancement by: (i) VisuMax Circle, followed by excimer ablation (S+C); (ii) secondary SMILE anterior to the primary procedure (S+SE); or (iii) surface ablation (S+P), and were examined for 28 days...
2017: PloS One
https://www.readbyqxmd.com/read/28704790/pharmacological-activities-of-an-eye-drop-containing-matricaria-chamomilla-and-euphrasia-officinalis-extracts-in-uvb-induced-oxidative-stress-and-inflammation-of-human-corneal-cells
#4
Elisabetta Bigagli, Lorenzo Cinci, Mario D'Ambrosio, Cristina Luceri
Ultraviolet B (UVB) exposure is a risk factor for corneal damage resulting in oxidative stress, inflammation and cell death. The aim of this study was to investigate the potential protective effects of a commercial eye drop (Dacriovis™) containing Matricaria chamomilla and Euphrasia officinalis extracts on human corneal epithelial cells (HCEC-12) against UVB radiation-induced oxidative stress and inflammation as well as the underlying mechanisms. The antioxidant potential of the eye drops was evaluated by measuring the ferric reducing antioxidant power and the total phenolic content by Folin-Ciocalteu reagent...
June 24, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28700779/corneal-wound-repair-after-rose-bengal-and-green-light-crosslinking-clinical-and-histologic-study
#5
Patricia Gallego-Muñoz, Lucía Ibares-Frías, Elvira Lorenzo, Susana Marcos, Pablo Peréz-Merino, Nandor Bekesi, Irene E Kochevar, M Carmen Martínez-García
Purpose: To evaluate corneal wound healing after treatment with a new collagen crosslinking protocol using rose bengal dye and green light (RGX). Methods: One cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter circle and, in another group (n = 25), the DE corneas were then stained with 0.1% rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes (RGX). The contralateral eyes without treatment acted as controls. The animals were clinically followed including fluorescein staining and pachymetry...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28691943/effect-of-a-new-matrix-therapy-agent-in-persistent-epithelial-defects-after-bacterial-keratitis-treated-with-topical-fortified-antibiotics
#6
Marc-Antoine Chappelet, Diane Bernheim, Christophe Chiquet, Florent Aptel
PURPOSE: To evaluate the effect of topical application of a matrix regenerating agent (RGTA) in subjects with a persistent epithelial defect after bacterial keratitis treated with topical fortified antibiotics. METHODS: In this prospective case series of 14 subjects (14 eyes) with a persisting corneal ulcer after the cessation of fortified antibiotics, subjects were treated with an RGTA at a dose of 1 drop every other day. The main outcome measure was the proportion of subjects with complete corneal healing 1 month after initiation of treatment...
July 6, 2017: Cornea
https://www.readbyqxmd.com/read/28688026/indications-and-graft-survival-analysis-in-optical-penetrating-keratoplasty-in-a-tertiary-care-center-in-north-india-a-5-year-study
#7
Sudesh Kumar Arya, Amit Raj, Ravi Kant Bamotra, Anubha Bhatti, Jyoti Deswal, Meenakshi Sindhu
PURPOSE: To study the indications and variables correlating with graft survival in optical penetrating keratoplasty in a tertiary care hospital in north India. PARTICIPANTS: All patients who underwent optical keratoplasty, except those undergoing lamellar grafts, tectonic grafts transplants and penetrating keratoplasty done for therapeutic purposes were included in the study. Patients with follow-up less than 2 years were excluded from the study. MATERIALS AND METHODS: Data were obtained by reviewing the records of 101 patients who underwent optical penetrating keratoplasty from 2008 to 2013 for various indications...
July 7, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28676989/in-vivo-and-ex-vivo-evaluation-of-cell-cell-interactions-adhesion-and-migration-in-ocular-surface-of-patients-undergone-excimer-laser-refractive-surgery-after-topical-therapy-with-different-lubricant-eyedrops
#8
Roberta Calienno, Claudia Curcio, Manuela Lanzini, Mario Nubile, Leonardo Mastropasqua
PURPOSE: To investigate, in vivo by means of in vivo confocal microscopy (IVCM) and ex vivo by impression cytology, epithelial cellular damage after excimer laser refractive surgery in patients under different topical lubricant therapies. METHODS: Two hundred eyes of 100 patients, undergone bilateral excimer laser refractive surgery for medium myopic error correction [spherical equivalent refraction from -1.75 to -3.50 dioptres (D) with refractive astigmatism under -0...
July 4, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28662184/corneal-epithelial-cells-exposed-to-shear-stress-show-altered-cytoskeleton-and-migratory-behaviour
#9
Sara Molladavoodi, Matthew Robichaud, David Wulff, Maud Gorbet
Cells that form the corneal epithelium, the outermost layer of the cornea, are exposed to shear stress through blinking during waking hours. In this in vitro study, the effect of fluid shear stress on human corneal epithelial cells (HCECs) was investigated. Following exposure to shear stresses of 4 and 8 dyn/cm2, HCECs showed cytoskeletal rearrangement with more prominent, organized and elongated filamentous actin. Cytoskeletal changes were time-dependent, and were most significant after 24 hours of shear stress...
2017: PloS One
https://www.readbyqxmd.com/read/28660128/medical-and-surgical-management-of-pasteurella-canis-infectious-keratitis
#10
Ankit Shah, Mansi Talati, Thomas Mauger
INTRODUCTION: We describe a case of refractory Pasteurella Canis keratitis that initially failed antimicrobial treatment but was successfully treated with a conjunctival flap followed by a penetrating keratoplasty. CASE DISCUSSION: A 70-year-old male had persistent corneal edema related to previous herpes zoster keratouveitis with visual acuity of 20/400. An endothelial keratoplasty was performed with improvement in vision to 20/25. Several months after this surgery the patient developed a corneal infiltrate and cultures grew Pasturella canis...
2017: IDCases
https://www.readbyqxmd.com/read/28660079/umbilical-cord-patch-transplantation-for-corneal-perforations-and-descemetoceles
#11
Hua-Tao Xie, Dan Zhao, Yang Liu, Ming-Chang Zhang
PURPOSE: To evaluate the clinical outcome of umbilical cord patch (UCP) transplantation for deep corneal ulcers with perforations and descemetoceles. METHODS: In this retrospective, noncomparative, interventional case series, 11 eyes of 11 patients with corneal perforation or descemetocele were included. The thickness and microstructure of UCP were measured. All eyes were treated with UCP and amniotic membrane transplantation for corneal reconstruction. Corneal ulcer healing, corneal thickness, anterior chamber formation, and best-corrected visual acuity (BCVA) were recorded and analyzed...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28659135/bilateral-morganella-morganii-keratitis-in-a-patient-with-facial-topical-corticosteroid-induced-rosacea-like-dermatitis-a-case-report
#12
Bei Zhang, Fei Pan, Kejian Zhu
BACKGROUND: Bilateral keratitis rarely occurs in individuals without predisposing factors. Here we describe the clinical course of a patient who developed a bilateral keratitis caused by Morganella. morganii which might be associated with long term using of topical corticosteroids-containing preparations on the face. CASE PRESENTATION: A 52-year-old female patient presented with marked bilateral corneal infiltration and hypopyon without any usual predisposing factors for bilateral infectious keratitis...
June 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28658821/intracorneal-amphotericin-b-injection-in-a-case-of-indolent-candidal-keratitis
#13
Jyoti Deswal, Sudesh Kumar Arya
Ocular candidiasis is one of the opportunistic infection occurring in human body. Pathogenesis wise, Candida is very virulent and slow growing organism. We report a case of 76-year-old female with left eye candidal stromal abscess, not responding to topical and oral antifungal drugs. Patient was administered intrastromal amphotericin B injection in the affected eye. Four midstromal injections were given in the areas surrounding the corneal abscess. A total of approximately 0.05 mL of amphotericin B, 5 μg per 0...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28657193/establishment-of-in-vitro-model-of-corneal-scar-pathophysiology
#14
Shikha Chawla, Sourabh Ghosh
Corneal scarring is the major source of permanent blindness worldwide. The complex pathophysiology of corneal scarring is not comprehensibly understood as it involves the interaction of a constellation of pro-fibrotic cytokines influencing several signaling pathways involved in corneal scar development. In the present study, an attempt has been made to generate a relatively simple in vitro corneal scar model using primary corneal keratocytes by exogenously providing an optimized dose of combination of cytokines (TGF-β1, IL-6 and IL-8) involved in scar formation in situ...
June 28, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28655773/ectodysplasin-a-promotes-corneal-epithelial-cell-proliferation
#15
Sanming Li, Jing Zhou, Jinghua Bu, Ke Ning, Liying Zhang, Juan Li, Yuli Guo, Xin He, Hui He, Xiaoxin Cai, Yongxiong Chen, Peter Sol Reinach, Zuguo Liu, Wei Li
The EDA gene encodes ectodysplasin A (Eda) which if mutated causes X-linked Hypohidrotic Ectodermal Dysplasia (XLHED) disease in humans. Ocular surface changes occur in XLHED patients while its underlying mechanism remains elusive. In this study, we found Eda was highly expressed in meibomian glands, and it was detected in human tears but not serum. Corneal epithelial integrity was defective and the thickness was reduced in the early postnatal stage of EDA mutant Tabby mice. Corneal epithelial cell proliferation decreased and the epithelial wound healing was delayed in Tabby mice, while it was restored by exogenous Eda...
June 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28644870/effects-of-nicotine-on-corneal-wound-healing-following-acute-alkali-burn
#16
Jong Won Kim, Chae Woong Lim, Bumseok Kim
Epidemiological studies have indicated that smoking is a pivotal risk factor for the progression of several chronic diseases. Nicotine, the addictive component of cigarettes, has powerful pathophysiological properties in the body. Although the effects of cigarette smoking on corneal re-epithelialization have been studied, the effects of nicotine on corneal wound healing-related neovascularization and fibrosis have not been fully demonstrated. The aim of this study was to evaluate the effects of chronic administration of nicotine on corneal wound healing following acute insult induced by an alkali burn...
2017: PloS One
https://www.readbyqxmd.com/read/28644681/treatment-results-of-corneal-collagen-cross-linking-combined-with-riboflavin-and-440-nm-blue-light-for-bacterial-corneal-ulcer-in-rabbits
#17
Shufang Wei, Cuiying Zhang, Shaoru Zhang, Yanyun Xu, Guoying Mu
PURPOSE: To study the treatment effect of corneal collagen cross-linking (CXL) combined with 440 nm blue light and riboflavin on bacterial corneal ulcer using animal experiments. METHODS: A total of 21 New Zealand white rabbits that developed Staphylococcus aureus corneal ulcer were randomly divided into three groups. Seven rabbits were used as blank control groups; seven rabbits were treated with CXL combined with riboflavin and 440 nm blue light; and seven rabbits were treated with CXL combined with riboflavin and 370 nm ultraviolet A light...
June 23, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28644234/efficacy-of-sodium-hyaluronate-in-murine-diabetic-ocular-surface-diseases
#18
Guohu Di, Xia Qi, Xiaowen Zhao, Songmei Zhang, Qingjun Zhou
PURPOSE: To evaluate the efficacy of sodium hyaluronate (HA) eye drops for the treatment of diabetic ocular surface diseases in mice. METHODS: Male 6- to 8-week-old C57BL/6 mice underwent induction of type 1 diabetes with intraperitoneal injections of streptozotocin, with normal mice as the control. Topical 0.3% HA, 0.1% HA, 0.4% polyethylene glycol eye drops, and normal saline were administered to diabetic mice with an intact or debrided corneal epithelium. Normal saline was applied in the controls...
June 21, 2017: Cornea
https://www.readbyqxmd.com/read/28632029/longitudinal-evaluation-of-wound-healing-after-penetrating-corneal-injury-anterior-segment-optical-coherence-tomography-study
#19
Kang Keng Zheng, Jianhao Cai, Shi Song Rong, Kun Peng, Honghe Xia, Chuan Jin, Xuehui Lu, Xinyu Liu, Haoyu Chen, Vishal Jhanji
PURPOSE: Ocular imaging can enhance our understanding of wound healing. We report anterior segment optical coherence tomography (ASOCT) findings in penetrating corneal injury. METHODS: Serial ASOCT was performed after repair of penetrating corneal injury. Internal aberrations of wound edges were labeled as "steps" or "gaps" on ASOCT images. The wound type was characterized as: type 1: continuous inner wound edge or step height ≤ 80 µm; type 2: step height > 80 µm; type 3: gap between wound edges; and type 4: intraocular tissue adherent to wound...
February 26, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28631887/thymic-stromal-lymphopoietin-is-expressed-in-human-corneal-stromal-cells-and-secreted-upon-protease-activated-receptor-1-activation
#20
Hongbo Yin, Yan Tian, Rongying Luo, Yingping Deng
Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine that triggers inflammatory responses through activating dendritic cell. It is demonstrated to be involved in a number of disorders, including cancer, gastrointestinal diseases, and autoimmune diseases. In addition, TSLP is also found to be able to induce itch sensation by directly activating the itch sensory neurons. The expression of human TSLP is detected in various tissues such as heart, liver, testis, and prostate. In this study, we report that TSLP was expressed in the human corneal stromal cells, the highly specialized cells that are critical for the function of the cornea...
June 20, 2017: IUBMB Life
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