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https://www.readbyqxmd.com/read/27639274/resources-and-recommendations-for-using-transcriptomics-to-address-grand-challenges-in-comparative-biology
#1
Donald L Mykles, Karen G Burnett, David S Durica, Blake L Joyce, Fiona M McCarthy, Carl J Schmidt, Jonathon H Stillman
High-throughput RNA sequencing (RNA-seq) technology has become an important tool for studying physiological responses of organisms to changes in their environment. De novo assembly of RNA-seq data has allowed researchers to create a comprehensive catalog of genes expressed in a tissue and to quantify their expression without a complete genome sequence. The contributions from the "Tapping the Power of Crustacean Transcriptomics to Address Grand Challenges in Comparative Biology" symposium in this issue show the successes and limitations of using RNA-seq in the study of crustaceans...
September 17, 2016: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/26279116/attempts-on-producing-lymphoid-cell-line-from-penaeus-monodon-by-induction-with-sv40-t-and-12s-eia-oncogenes
#2
Jayesh Puthumana, Priyaja Prabhakaran, Rosamma Philip, I S Bright Singh
In an attempt of in vitro transformation, transfection mediated expression of Simian virus-40 (T) antigen (SV40-T) and transduction mediated expression of Adenovirus type 12 early region 1A (12S E1A) oncogene were performed in Penaeus monodon lymphoid cells. pSV3-neo vector encoding SV40-T oncogene and a recombinant baculovirus BacP2-12S E1A-GFP encoding 12S E1A oncogene under the control of hybrid promoters were used. Electroporation and lipofection mediated transformation of SV40-T in lymphoid cells confirmed the transgene expression by phenotypic variation and the expression of GFP in co-transfection experiment...
December 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/25982944/transgene-expression-in-penaeus-monodon-cells-evaluation-of-recombinant-baculoviral-vectors-with-shrimp-specific-hybrid-promoters
#3
Jayesh Puthumana, Rosamma Philip, I S Bright Singh
It has been realized that shrimp cell immortalization may not be accomplished without in vitro transformation by expressing immortalizing gene in cells. In this process, efficiency of transgene expression is confined to the ability of vectors to transmit gene of interests to the genome. Over the years, unavailability of such vectors has been hampering application of such a strategy in shrimp cells. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of Penaeus monodon...
August 2016: Cytotechnology
https://www.readbyqxmd.com/read/25835297/function-and-regulation-domains-of-a-newly-isolated-putative-%C3%AE-actin-promoter-from-pacific-white-shrimp
#4
Yingli Shi, Marcus Soderlund, Jianhai Xiang, Yuanan Lu
Current development of transgenic shrimp research has been hampered due to the lack of the suitable promoters and efficient transfection methods for crustaceans. A 1642 bp sequence, containing 5'-upstream sequence, exon 1, intron 1 and partial exon 2, which is responsible for transcriptional initiation of the newly reported shrimp β-actin (actinT1), has been isolated from the Pacific white shrimp (Litopenaeus vannamei) and named as SbaP. To determine its function and potential application in marine biotechnology, the sequence and functional domains were examined by constitutive expression of the luciferase reporter gene...
2015: PloS One
https://www.readbyqxmd.com/read/25462461/zebrafish-fed-on-recombinant-artemia-expressing-epinecidin-1-exhibit-increased-survival-and-altered-expression-of-immunomodulatory-genes-upon-vibrio-vulnificus-infection
#5
Yu-Hsuan Jheng, Lin-Han Lee, Chen-Hung Ting, Chieh-Yu Pan, Cho-Fat Hui, Jyh-Yih Chen
Artemia has been used extensively in aquaculture as fodder for larval fish, shrimp, and shellfish. Epinecidin-1, an antimicrobial peptide, was isolated from grouper (Epinephelus coioides) in 2005. Epinecidin-1 has been previously reported to possess antimicrobial activity against several Gram-positive and Gram-negative bacterial species, including Staphylococcus coagulase, Pseudomonas aeruginosa, Streptococcus pyogenes, and Vibrio vulnificus. In this study, we used electroporation to introduce plasmid DNA encoding a green fluorescent protein (EGFP)-epinecidin-1 fusion protein under the control of the cytomegalovirus (CMV) promoter into decapsulated Artemia cysts...
January 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/24211341/identification-characterization-and-functional-analysis-of-a-serine-protease-inhibitor-lvserpin-from-the-pacific-white-shrimp-litopenaeus-vannamei
#6
Yongjie Liu, Fujun Hou, Shulin He, Zhaoying Qian, Xianzong Wang, Aitao Mao, Chengbo Sun, Xiaolin Liu
As important arthropod immune responses, prophenoloxidase (proPO) activation and Toll pathway initiation are mediated by serine proteinase cascades and regulated by serpins. Herein, a serine protease inhibitor (Lvserpin), encoding for 415 amino acids with calculated molecular weight of 46,639 Da and isoelectric point of 7.03 was characterized from the Pacific white shrimp Litopenaeus vannamei. Multiple sequence alignment revealed that Lvserpin shared the highest similarity with Penaeus monodon serpin6 (87%)...
March 2014: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/24176974/the-immune-responses-triggered-by-cpg-odns-in-shrimp-litopenaeus-vannamei-are-associated-with-lvtolls
#7
Rui Sun, Mengqiang Wang, Lingling Wang, Feng Yue, Qilin Yi, Mengmeng Huang, Rui Liu, Limei Qiu, Linsheng Song
CpG oligodeoxynucleotides (ODNs) represent a kind of pathogen-associated molecular patterns (PAMPs) as well as a novel adjuvant that activate the innate immune system through interaction with Toll-like receptor 9 (TLR9) in mammals. In the present study, the synthetic oligodeoxynucleotides, CpG ODN 2395, was employed to investigate the interactive mode of CpG ODNs with three known Tolls (LvToll1-3) from shrimp Litopenaeus vannamei. The mature peptides of extracellular domains of LvTolls (LvToll-ECDs) were recombinant expressed and their binding activities to CpG ODN 2395 were further examined by ELISA...
March 2014: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/24081826/preparation-of-transgenic-dunaliella-salina-for-immunization-against-white-spot-syndrome-virus-in-crayfish
#8
Shuying Feng, Wenpo Feng, Ling Zhao, Huihui Gu, Qinghua Li, Ke Shi, Sanxing Guo, Nannan Zhang
Although a white spot syndrome virus (WSSV) subunit vaccine could significantly enhance the immune response and benefit the shrimp host, its practical application is currently not feasible because of drawbacks in existing expression systems. We generated a transgenic Dunaliella salina (D. salina) strain by introducing the WSSV VP28 gene to produce a novel oral WSSV subunit vaccine. Following transformation of D. salina, VP28 gene expression was assessed by reverse transcription polymerase chain reaction (RT-PCR) assays, enzyme-linked immunosorbent assays (ELISAs), and western blot analysis...
March 2014: Archives of Virology
https://www.readbyqxmd.com/read/21931807/expression-of-a-novel-antimicrobial-peptide-penaeidin4-1-in-creeping-bentgrass-agrostis-stolonifera-l-enhances-plant-fungal-disease-resistance
#9
Man Zhou, Qian Hu, Zhigang Li, Dayong Li, Chin-Fu Chen, Hong Luo
BACKGROUND: Turfgrass species are agriculturally and economically important perennial crops. Turfgrass species are highly susceptible to a wide range of fungal pathogens. Dollar spot and brown patch, two important diseases caused by fungal pathogens Sclerotinia homoecarpa and Rhizoctonia solani, respectively, are among the most severe turfgrass diseases. Currently, turf fungal disease control mainly relies on fungicide treatments, which raises many concerns for human health and the environment...
2011: PloS One
https://www.readbyqxmd.com/read/21855569/a-comparative-study-of-antimicrobial-properties-of-crustinpm1-and-crustinpm7-from-the-black-tiger-shrimp-penaeus-monodon
#10
COMPARATIVE STUDY
Kuakarun Krusong, Pasapong Poolpipat, Premruethai Supungul, Anchalee Tassanakajon
Several isoforms of crustin have been identified in the black tiger shrimp Penaeus monodon. These cationic cysteine-rich antimicrobial peptides contain a single whey acidic protein (WAP) domain at the C-terminus and exhibit antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this paper, we investigate the binding properties and antimicrobial actions of crustinPm1 and crustinPm7, the two most abundant crustin isoforms found in the haemocyte of P. monodon. Previously, crustinPm1 showed strong inhibition against Gram-positive bacteria, whilst crustinPm7 acted against both Gram-positive and Gram-negative bacteria...
January 2012: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/21642002/transgenic-expression-of-tilapia-hepcidin-1-5-and-shrimp-chelonianin-in-zebrafish-and-their-resistance-to-bacterial-pathogens
#11
Chieh-Yu Pan, Kuan-Chieh Peng, Cheng-Hui Lin, Jyh-Yih Chen
Recently, tilapia hepcidin (TH)1-5 was characterized, and its antimicrobial functions against several pathogens were reported. The antimicrobial functions of another shrimp antimicrobial peptide (AMP), chelonianin, were also characterized using a recombinant chelonianin protein (rcf) that was expressed by a stably transfected Chinese hamster ovary (CHO) cell line against pathogen infections in fish. The function of the overexpression of both AMPs in zebrafish muscles was not examined in previous studies. Herein, we investigated the antimicrobial functions of TH1-5 and chelonianin against Vibrio vulnificus (204) and Streptococcus agalactiae (SA48) in transgenic TH1-5 zebrafish and transgenic chelonianin zebrafish...
August 2011: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/19702854/development-of-paratransgenic-artemia-as-a-platform-for-control-of-infectious-diseases-in-shrimp-mariculture
#12
B Subhadra, I Hurwitz, A Fieck, D V S Rao, G Subba Rao, R Durvasula
AIM: To study the accumulation and retention of recombinant proteins in Artemia gut for optimizing paratransgenic disease control in shrimp aquaculture. METHODS AND RESULTS: Transgenic Escherichia coli expressing fluorescent marker proteins and the transgenic cyanobacterium Synechococcus bacillarus expressing a functional murine single chain antibody, DB3, were fed to Artemia franciscana. Stable expression and retention of several marker molecules (e.g. GFP, DS Red and DB3) up to 10 h after of feeding with E...
March 2010: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/18644734/transgenic-carrot-plants-accumulating-ketocarotenoids-show-tolerance-to-uv-and-oxidative-stresses
#13
Jayaraman Jayaraj, Zamir K Punja
Ketocarotenoids are strong antioxidant compounds which accumulate in salmon, shrimp, crustaceans and algae, but are rarely found naturally in higher plants. In this study, we engineered constitutive expression of an algal beta-carotene ketolase gene (bkt) in carrot plants to produce a number of ketocarotenoids from beta-carotene. These included astaxanthin, adonirubin, canthaxanthin, echinenone, adonixanthin and beta-cryptoxanthin. Leaves accumulated up to 56 microg/g total ketocarotenoids and contained higher beta-carotene levels but lower levels of alpha-carotene and lutein...
October 2008: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/18204820/growth-enhancement-of-shrimp-litopenaeus-schmitti-after-transfer-of-tilapia-growth-hormone-gene
#14
Amilcar Arenal, Rafael Pimentel, Eulogio Pimentel, Leonardo Martín, Dayamí Santiesteban, Ramón Franco, Peter Aleström
Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyses. Western blot analyses revealed that 34% of the screened larvae expressed a single tiGH-specific band with the expected molecular mass (23.1 kDa). The development index and larval length indicated a significant growth enhancement from day 3 on after electroporation, with an average of 32% of the growth enhancement...
May 2008: Biotechnology Letters
https://www.readbyqxmd.com/read/16129499/viral-resistance-in-shrimp-that-express-an-antisense-taura-syndrome-virus-coat-protein-gene
#15
Yuanan Lu, Piera S Sun
Taura syndrome virus (TSV) is a major cause of mortality and morbidity in shrimp, and has a profound economic impact on commercial U.S. shrimp farming. This paper describes the stable expression of an antisense Taura syndrome virus-coat protein (TSV-CP) gene construct in shrimp zygotes, via transfection using jetPEI reagent, over a period of at least 236 days. The transgenic shrimp showed no statistically significant difference from normal control shrimp in terms of weight gain or their appearance, morphology, swimming and eating activities...
September 2005: Antiviral Research
https://www.readbyqxmd.com/read/11191866/introducing-foreign-dna-into-tiger-shrimp-penaeus-monodon-by-electroporation
#16
F S Tseng, H J Tsai, I C Liao, Y L Song
Electroporation was used to introduce pFLAG-CMV-1-BAP, a DNA fragment that includes a bacterial alkaline phosphatase gene driven by a human cytomegalovirus (CMV) promoter, into Penaeus monodon zygotes. The transgenic tiger shrimp was achievedby using 10kV, 28 pulses, 120 g sec pulse time, 10 cycles, and a DNA concentration of 37.5 microg/mL. The hatching rate of electroporated zygotes (46%) was significantly lower than that of zygotes in the untreated group (89%). The survival rate of postlarvae in the electroporated group using a DNA concentration of 37...
December 1, 2000: Theriogenology
https://www.readbyqxmd.com/read/8541979/future-of-biotechnology-based-control-of-disease-in-marine-invertebrates
#17
E Mialhe, E Bachere, V Boulo, J P Cadoret, C Rousseau, V Cedeño, E Saraiva, L Carrera, J Calderon, R R Colwell
Infectious disease is the single most devastating problem in mollusc and shrimp aquaculture. Pathogens causing the greatest problems have been identified as viruses, prokaryotes, and protozoans. Two approaches employing methods of biotechnology have been proposed to prevent, manage, and control mollusc and shrimp diseases. The first is development of a diagnostic scheme for detection and identification of pathogens, using molecular probes. This offers the opportunity for prophylactic measures to be taken. Molecular probes have been prepared for the major pathogens of molluscs, but in the case of shrimp pathogens, only a few are available...
December 1995: Molecular Marine Biology and Biotechnology
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