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https://www.readbyqxmd.com/read/28632911/polymer-functionalized-gold-nanoparticles-as-non-viral-gene-delivery-reagents
#1
M Mar Encabo-Berzosa, Maria Sancho-Albero, Victor Sebastian, Silvia Irusta, Manuel Arruebo, Jesus Santamaria, Pilar Martín Duque
BACKGROUND: In this study we investigated the ability of PEG functionalized gold nanoparticles as non-viral vectors in the transfection of different cell lines, comparing them with commercial lipoplexes. METHODS: Positively charged gold nanoparticles were synthesized using PEI as reducing and stabilizer agent and its cytotoxicity reduced by its functionalization with PEG. We bound the nanoparticles to three plasmids with different sizes (4-40 kpb). The vector internalization was evaluated by confocal and electronic microscopy...
June 20, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28631386/toward-establishing-model-organisms-for-marine-protists-successful-transfection-protocols-for-parabodo-caudatus-kinetoplastida-excavata
#2
Fatma Gomaa, Paulo A Garcia, Jennifer Delaney, Peter R Girguis, Cullen R Buie, Virginia P Edgcomb
We developed protocols for, and demonstrated successful transfection of, the free-living kinetoplastid flagellate Parabodo caudatus with three plasmids carrying a fluorescence reporter gene (pEF-GFP with the EF1 alpha promoter, pUB-GFP with Ubiquitin C promoter, and pEYFP-Mitotrap with CMV promoter). We evaluated three electroporation approaches: 1) a square-wave electroporator designed for eukaryotes, 2) a novel microfluidic transfection system employing hydrodynamically-controlled electric field waveforms, and 3) a traditional exponential decay electroporator...
June 19, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28629080/efficient-megalin-targeted-delivery-to-renal-proximal-tubular-cells-mediated-by-modified-polymyxin-b-polyethylenimine-based-nano-gene-carriers
#3
Fatemeh Oroojalian, Ali Hossein Rezayan, Faramarz Mehrnejad, Azadeh Hashem Nia, Wayne Thomas Shier, Khalil Abnous, Mohammad Ramezani
Non-viral vectors have attracted great interest, as they are simple to prepare, easy to modify and relatively safe, compared to viral vectors. Kidney-targeted gene delivery systems depict a promising technology to improve drug efficacy in renal diseases treatments. In order to develop a novel kidney-targeted gene delivery system, we synthesized polyamine-PEI conjugates using polymyxin B as ligand and investigated their potential targeting efficiency. After grafting either PEI25 kDa or PEI10 kDa with polymyxin B through amidation reaction, the modified-polymyxin-PEI/DNA-nanoplexes were produced via electrostatic attraction between the cationic polymers and EGFP plasmid...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28624218/targeted-multifunctional-lipid-eco-plasmid-dna-nanoparticles-as-efficient-non-viral-gene-therapy-for-leber-s-congenital-amaurosis
#4
Da Sun, Bhubanananda Sahu, Songqi Gao, Rebecca M Schur, Amita M Vaidya, Akiko Maeda, Krzysztof Palczewski, Zheng-Rong Lu
Development of a gene delivery system with high efficiency and a good safety profile is essential for successful gene therapy. Here we developed a targeted non-viral delivery system using a multifunctional lipid ECO for treating Leber's congenital amaurosis type 2 (LCA2) and tested this in a mouse model. ECO formed stable nanoparticles with plasmid DNA (pDNA) at a low amine to phosphate (N/P) ratio and mediated high gene transfection efficiency in ARPE-19 cells because of their intrinsic properties of pH-sensitive amphiphilic endosomal escape and reductive cytosolic release (PERC)...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28622054/down-regulation-of-liver-intestine-cadherin-enhances-noscapine-induced-apoptosis-in-human-colon-cancer-cells
#5
Xia Tian, Meng Liu, Qingxi Zhu, Jie Tan, Weijie Liu, Yanfen Wang, Wei Chen, Yanli Zou, Yishan Cai, Zheng Han, Xiaodong Huang
OBJECTIVES: Liver-intestine cadherin (CDH17) as a novel type of cadherin can significantly suppresses tumor growth in colon cancer cells. Noscapine is one of the most widely used antineoplastic agents in the treatment of solid tumor and haematological malignancies. The aim of the present study was to explore the signaling pathway of noscapine which induces apoptosis by blocking CDH17 gene in colon cancer SW480 cells. METHODS: Human colon cancer SW480 cells were transfected with CDH17 interference vector and treatment with 10 µmol/L noscapine...
June 16, 2017: Expert Review of Anticancer Therapy
https://www.readbyqxmd.com/read/28615075/reduced-rce1-expression-predicts-poor-prognosis-of-colorectal-carcinoma
#6
Boyun Shi, Xinke Zhou, Lu He, Min Liang, Yuanwei Luo, Peng Jiang
BACKGROUND: As an end-proteolytic enzyme that cleaves the last three residues of proteins with a terminal CAAX, Ras-converting enzyme 1 (RCE1) has an essential role in multiple signaling pathways and take part in the process of differentiation, proliferation and carcinogenesis. The aim of the study is to investigate expression pattern of RCE1 and its prognosis in colorectal carcinoma (CRC). METHODS: The expression of RCE1 and phospho-MAPK family members was confirmed by immunohistochemical staining of CRC tissues...
June 14, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28611246/inhibition-of-avian-influenza-a-virus-replication-in-human-cells-by-host-restriction-factor-tufm-is-correlated-with-autophagy
#7
Shu-Ming Kuo, Chi-Jene Chen, Shih-Cheng Chang, Tzu-Jou Liu, Yi-Hsiang Chen, Sheng-Yu Huang, Shin-Ru Shih
Avian influenza A viruses generally do not replicate efficiently in human cells, but substitution of glutamic acid (Glu, E) for lysine (Lys, K) at residue 627 of avian influenza virus polymerase basic protein 2 (PB2) can serve to overcome host restriction and facilitate human infectivity. Although PB2 residue 627 is regarded as a species-specific signature of influenza A viruses, host restriction factors associated with PB2627E have yet to be fully investigated. We conducted immunoprecipitation, followed by differential proteomic analysis, to identify proteins associating with PB2627K (human signature) and PB2627E (avian signature) of influenza A/WSN/1933(H1N1) virus, and the results indicated that Tu elongation factor, mitochondrial (TUFM), had a higher binding affinity for PB2627E than PB2627K in transfected human cells...
June 13, 2017: MBio
https://www.readbyqxmd.com/read/28607147/loss-of-the-clock-protein-per2-shortens-the-erythrocyte-life-span-in-mice
#8
Qi Sun, Yue Zhao, Yunxia Yang, Xiao Yang, Minghui Li, Xi Xu, Dan Weng, Junsong Wang, Jianfa Zhang
Cell proliferation and release from the bone marrow have been demonstrated to be controlled by circadian rhythms in both humans and mice. However, it is unclear whether local circadian clocks in the bone marrow influence physiological functions and life span of erythrocytes. Here, we report that loss of the clock gene Per2 significantly decreased erythrocyte life span. Mice deficient in Per2 were more susceptible to acute stresses in the erythrocytes, becoming severely anemic upon phenylhydrazine, osmotic, and H2O2 challenges...
June 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28601080/microrna-630-suppresses-epithelial-to-mesenchymal-transition-by-regulating-foxm1-in-gastric-cancer-cells
#9
Jing Feng, Xiaojuan Wang, Weihua Zhu, Si Chen, Changwei Feng
In the present study, we investigated the functional role of microRNA (miR)-630 in epithelial-to-mesenchymal transition (EMT) of gastric cancer (GC) cells, as well as the regulatory mechanism. Cells of human GC cell line SGC 7901 were transfected with miR-630 mimic or miR-630 inhibitor. The transfection efficiency was confirmed by qRT-PCR. Cell migration and invasion were determined by Transwell assay. Protein expression of E-cadherin, vimentin, and Forkhead box protein M1 (FoxM1) was tested by Western blot...
June 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28600130/highly-antibacterial-polypeptide-based-amphiphilic-copolymers-as-multifunctional-non-viral-vectors-for-enhanced-intracellular-sirna-delivery-and-anti-infection
#10
Li Zhou, Yuewei Xi, Meng Yu, Min Wang, Yi Guo, Peng Li, Peter X Ma, Bo Lei
For next-generation regenerative medicine and cancer therapy applications, the development of multifunctional biodegradable biomaterials with good antibacterial activity, biocompatibility and gene delivery capability is showing increased interests. As a natural cationic polypeptide, poly(ε-L-lysine) (EPL) has been as attractive antibacterial agent and non-viral gene vectors, however, its gene therapy is still limited probably due to the cytotoxicity, low stability in physiological environment and poor transfection efficiency...
June 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28597662/chitosan-hyaluronic-acid-nanoparticles-rational-design-revisited-for-rna-delivery
#11
Enrique Lallana, Julio M Rios de la Rosa, Annalisa Tirella, Maria Pelliccia, Arianna Gennari, Ian J Stratford, Sanyogitta Puri, Marianne Ashford, Nicola Tirelli
Chitosan/hyaluronic acid (HA) nanoparticles can be used to deliver an RNA/DNA cargo to cells overexpressing HA receptors such as CD44. For these systems, unequivocal links have not been established yet between chitosan macromolecular (molecular weight; degree of deacetylation, i.e., charge density) and nanoparticle variables (complexation strength, i.e., stability; nucleic acid protection; internalization rate) on one hand, and transfection efficiency on the other hand. Here, we have focused on the role of avidity on transfection efficiency in the CD44-expressing HCT-116 as a cellular model; we have employed two differently sized payloads (a large luciferase-encoding mRNA and a much smaller anti-Luc siRNA), and a small library of chitosans (variable molecular weight and degree of deactylation)...
June 19, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28595094/two-plasmid-system-to-increase-the-rescue-efficiency-of-paramyxoviruses-by-reverse-genetics-the-example-of-rescuing-newcastle-disease-virus
#12
Haijin Liu, Emmanuel Albina, Patricia Gil, Cécile Minet, Renata Servan de Almeida
Within paramyxoviruses, conventional reverse genetics require the transfection of a minimum of four plasmids: three to reconstruct the viral polymerase complex that replicates and expresses the virus genome delivered by a fourth plasmid. The successful transfection of four or more plasmids of different sizes into one cell and the subsequent generation of at least one viable and replicable viral particle is a rare event, which explains the low rescue efficiency, especially of low virulent viruses with reduced replication efficiency in cell lines...
June 5, 2017: Virology
https://www.readbyqxmd.com/read/28592924/suppression-of-endothelial-cell-migration-by-tumor-associated-macrophage-derived-exosomes-is-reversed-by-epithelial-ovarian-cancer-exosomal-lncrna
#13
Quanfeng Wu, Xiaoli Wu, Xiang Ying, Qinyi Zhu, Xinjing Wang, Lu Jiang, Xin Chen, Yueqian Wu, Xipeng Wang
OBJECTIVE: To study the mechanism by which epithelial ovarian cancer (EOC)-derived exosomes restore the migration of endothelial cells that is suppressed by TAM-derived exosomes. METHODS: Exosomes were isolated from TAMs in the ascites of patients with EOC. The effect of exosomes on the expression of endothelial cell miRNA was monitored by PCR. The miRNA mimics were transfected to explore their effects. Microarray data and literature searches were used to predict target genes and the impact of target gene pathways, and small interfering RNA was used to target these genes...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28586046/enhanced-effect-of-nuclear-localization-signal-peptide-during-ultrasound%C3%A2-targeted-microbubble-destruction%C3%A2-mediated-gene-transfection
#14
Sheng Cao, Qing Zhou, Jin-Ling Chen, Nan Jiang, Yi-Jia Wang, Qing Deng, Bo Hu, Rui-Qiang Guo
Ultrasound‑targeted microbubble destruction (UTMD) can promote the entry of plasmid DNA (pDNA) into the cell cytoplasm, by increasing the permeability of the cell membrane. But the transfection efficiency remains low due to inability of the pDNA to enter the nucleus. Various methods have been explored to improve the UTMD transfection efficiency, but with little success. In cells, the classic nuclear localization signal (cNLS) peptide is an amino acid sequence that signals proteins that are due for nuclear transport...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28580308/a-novel-approach-on-drug-delivery-investigation-of-a-new-nano-formulation-of-liposomal-doxorubicin-and-biological-evaluation-of-entrapped-doxorubicin-on-various-osteosarcoma-cell-lines
#15
Fateme Haghiralsadat, Ghasem Amoabediny, Mohammad Hasan Sheikhha, Tymour Forouzanfar, Marco N Helder, Behrouz Zandieh-Doulabi
OBJECTIVE: In this study we prepared a novel formulation of liposomal doxorubicin (L- DOX). The drug dose was optimized by analyses of cellular uptake and cell viability of osteosarcoma (OS) cell lines upon exposure to nanoliposomes that contained varying DOX concentrations. We intended to reduce the cytotoxicity of DOX and improve characteristics of the nanosystems. MATERIALS AND METHODS: In this experimental study, we prepared liposomes by the pH gradient hydration method...
2017: Cell Journal
https://www.readbyqxmd.com/read/28578643/glioblastoma-targeted-gene-therapy-based-on-pegfp-p53-loaded-superparamagnetic-iron-oxide-nanoparticles
#16
Touba Eslaminejad, Seyed Noureddin Nematollahi-Mahani, Mehdi Ansari
BACKGROUND: Bloodâ€"brain barrier (BBB) separates the neural tissue from circulating blood because of its high selectivity. This study focused on the in vitro application of magnetic nanoparticles to deliver Tp53 as a gene of interest to glioblastoma (U87) cells across a simulated BBB model that comprised KB cells. MATERIAL AND METHOD: After magnetic and non-magnetic nanoparticles were internalized by KB cells, their location in these cells was examined by transmission electron microscopy...
June 4, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28565841/ultrasound-combined-with-targeted-cationic-microbubble-mediated-angiogenesis-gene-transfection-improves-ischemic-heart-function
#17
Qing Zhou, Qing Deng, Bo Hu, Yi-Jia Wang, Jin-Ling Chen, Jing-Jing Cui, Sheng Cao, Hong-Ning Song
The present study aimed to construct targeted cationic microbubbles (TCMBs) by synthesizing cationic microbubbles conjugated to an intercellular adhesion molecule-1 (ICAM-1) antibody, and then to use the TCMBs to deliver the angiopoietin-1 (Ang-1) gene into infarcted heart tissue using ultrasound-mediated microbubble destruction. It was hypothesized that the TCMBs would accumulate in higher numbers than non-targeted cationic microbubbles (CMBs) in the infarcted heart, and would therefore increase the efficiency of targeted Ang-1 gene transfection and promote angiogenesis...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28560395/hsvtk-gcv-system-on-hepatoma-carcinoma-cells-construction-of-the-plasmid-pcdna3-1%C3%A2-pafp-tk-and-targeted-killing-effect
#18
Yong-Fang Li, Yang-Yang Yuan, Ying-Min Zhang, Na Zhao, Qi Zhang, Fan-Xiu Meng, Ran-Peng Gao, Bao-Feng Yu, Yue-Hong Zhang, Rui Guo, Hai-Long Wang, Jun Xie, Jun Xu, Qin Qin, Xiu-Shan Dong
Previous studies demonstrated that herpes simplex virus thymidine kinase (HSVtk) could phosphorylate non‑toxic gancyclovir (GCV) efficiently to produce phosphorylated products that result in cell apoptosis, to kill tumor cells. The present study aimed to construct a plasmid vector, pcDNA3.1‑pAFP‑TK, carrying the suicide gene driven by the alpha‑fetoprotein (AFP) promoter, to investigate the cytotoxicity of HSVtk/GCV suicide gene system on hepatoma carcinoma cells. Reverse transcription‑polymerase chain reaction and western blotting results demonstrated that the HSVtk gene was effectively expressed in HepG2 hepatoma carcinoma cells transfected with pcDNA3...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28557288/the-ef-1%C3%AE-promoter-maintains-high-level-transgene-expression-from-episomal-vectors-in-transfected-cho-k1-cells
#19
Xiaoyin Wang, Chongjie Xu, Zhengwei Tian, Xi Zhang, Danhua Xu, Qin Li, Junhe Zhang, Tianyun Wang
In our previous study, we demonstrated that episomal vectors based on the characteristic sequence of matrix attachment regions (MARs) and containing the cytomegalovirus (CMV) promoter allow transgenes to be maintained episomally in Chinese hamster ovary (CHO) cells. However, the transgene expression was unstable and the number of copies was low. In this study, we focused on enhancers, various promoters and promoter variants that could improve the transgene expression stability, expression magnitude (level) and the copy number of a MAR-based episomal vector in CHO-K1 cells...
May 30, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28552376/involvement-of-stat1-in-proliferation-and-invasiveness-of-trophoblastic-cells
#20
Francisco Lázaro Pereira de Sousa, Wittaya Chaiwangyen, Diana M Morales-Prieto, Stephanie Ospina-Prieto, Maja Weber, Stella M Photini, Nelson Sass, Silvia Daher, Ekkehard Schleussner, Udo R Markert
Trophoblast proliferation and invasion are controlled by cytokines and growth factors present at the implantation site. Members of the Interleukin-6 (IL-6) family of cytokines trigger their effects through activation of intracellular cascades including the Janus Kinase/Signal Transducer and Activator of Transcription (JAK-STAT) pathway. Functions of several STAT molecules in trophoblast cells have been described, but the role of STAT1 remained unclear. Here, potential functions of STAT1 and its activation by Oncostatin M (OSM) have been investigated in an in vitro model...
May 25, 2017: Reproductive Biology
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