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https://www.readbyqxmd.com/read/27356893/proteomic-analysis-of-exosomes-and-exosome-free-conditioned-media-derived-from-human-osteosarcoma-cell-lines-reveal-differential-secretion-of-proteins-related-to-biological-functions-and-tumor-progression
#1
Sofia Jerez, Andre J van Wijnen, Mario Galindo
Osteosarcoma is an aggressive bone tumor affecting childhood. Currently there are a few proteomic studies analyzing secreted proteins in osteosarcoma. To characterize the osteosarcoma secretome, secreted proteins in the conditioned media of three human osteosarcoma cell lines were analyzed. Exosomes and exosome-free supernatants containing soluble proteins were isolated and characterized. Exosomes were analyzed by transmission electron microscopy (TEM) and western blot. Exosomal and soluble proteins (under 70-100 kDa, respectively) were identified by mass spectrometry analysis using nanoLC-MS/MS...
June 30, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/26711214/-relationship-of-gstp1-lower-expression-and-multidrug-resistance-reversing-of-curcumin-on-human-colon-carcinoma-cells
#2
He Li, Lei Li
OBJECTIVE: To explore the proteomic differences among with and without curcumin treatment of vincristine-resistance HCT-8/VCR cells of human colon carcinoma by using mass spectrometry and two-dimensional gel electrophore-sis (2-DE). METHODS: The total proteins of the both groups were extracted from serum were run in immobilized pH gradient isoelectic focusing (IPG-IEF) at the first dimension.The proteins pots in gels were visualized by silver staining protocol, scanned by using a molecular imager GS-800 calibrated densitometer...
August 11, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/26524029/-role-of-cyclind1-igh-detection-by-fish-in-differential-diagnostic-significance-between-mantle-cell-lymphoma-and-chronic-lymphocytic-leukemia
#3
Guo-Ping Li, Wan-Zi Chen, Hui-Fang Huang, Jia-Di Chen, Xiao-Lan Lin, Qiang Fu
OBJECTIVE: To investigate the feasibility of CyclinD1/IgH detection by FISH in diferential diagnosis between mantle cell lymphoma (MCL) and chronic lymphocytic leukeamia (CLL). METHODS: The FISH detection was performed for CyclinD1/IgH fusion gene. A comprehensive analysis was carried out for clinical features, such as age, sex , WBC count and lymphocyte count, the bone marrow morphology and immunohistochemical staining were carried for CyclinD1/IgH. RESULTS: It is often difficult to distinguish MCL from CLL by bone marrow morphology, when the cell morphology was not typical; there was no difference in age, sex, WBC count and lymphocyte count between MCL and CLL groups; 9 out of 52 patients were diagnosed as MCL, and the direction of CyclinD1/IgH by FISH was positive in 7 of 9 MCL, while 3 of the 7 patients were negative by immunohistochemical staining for CyclinD1...
October 2015: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/26387386/the-effects-of-tnf-%C3%AE-on-osteogenic-differentiation-of-umbilical-cord-derived-mesenchymal-stem-cells
#4
Kulisara Marupanthorn, Chairat Tantrawatpan, Duangrat Tantikanlayaporn, Pakpoom Kheolamai, Sirikul Manochantr
Mesenchymal stem cells (MSCs) are multipotent stem cells which are able to differentiate into various lineages including osteoblasts, adipocytes and chondrocytes. They can be isolated from several tissues including bone marrow, adipose tissue, placenta and umbilical cord. Although MSCs could be diferentiated into osteoblasts under appropriate culture condition, their osteogenic differentiation capacity is still not very efficient. Previous studies reported that TNF-α could promote osteogenic differentiation of bone marrow derived MSCs by triggering NF-κB signaling pathway...
April 2015: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
https://www.readbyqxmd.com/read/26384122/central-giant-cell-granuloma-located-in-the-maxilla-in-a-8-year-old-boy
#5
Anna Janas, Piotr Osica
UNLABELLED: Central giant cell granuloma is a rare occurring tumor-like lesion of the facial part of the skull, located more often in mandible (from 2:1 to 3:1), than in the maxilla. It accounts for 7% of all benign tumors of the jaws, whereas the brown tumor is a complication of a primary hyperparathyroidism and occurs in approximately 4.5%, or in 1.5%, when the tumor is a complication of secondary hyperparathyroidism. However, the brown tumor is significantly less often observed than the presented lesion...
April 2015: Developmental Period Medicine
https://www.readbyqxmd.com/read/23929086/retinal-vasoproliferative-tumor
#6
Eduardo Ferrari Marback, Ricardo Leitão Guerra, Otacilio de Oliveira Maia Junior, Roberto Lorens Marback
Retinal vasoproliferative tumor is a rare disease that has capillary hemangioma as the most frequent diferential diagnosis. The tumor is considered to be of reactive nature. It can be idiophatic or secondary to other ocular diseases such as: uveitis, retinitis pigmentosa, sickle cell disease, previous surgery and retinopathy of prematurity. Lesions with no exsudation or visual decrease can be observed. Lesions that need treatment can be managed by on or more modalities such as cryotherapy, a variety of lasers, surgical excision, radiation, and antiangiogenic intravitreal injections...
May 2013: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/23797988/diferential-dna-synthesis-in-anopheles-albimanus-tissues-induced-by-immune-challenge-with-different-microorganisms
#7
Salvador Hernández-Martínez, Didier Barradas-Bautista, Mario H Rodríguez
The induction of DNA synthesis in various tissues of Anopheles albimanus, in response to challenge with Saccharomyces cerevisiae, Micrococcus luteus, and Serratia marcescens, was analyzed by 5-bromo-2-deoxy-uridine (BrdU) incorporation. Microorganism-inoculated mosquitoes were fed with a sucrose solution containing BrdU and maintained alive for 5 days. Alternatively, abdominal carcasses of microorganisms-inoculated mosquitoes were cultivated in Roswell Park Memorial Institute (RPMI) medium supplemented with BrdU for 5 days...
September 2013: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/23335855/genes-induced-by-reovirus-infection-have-a-distinct-modular-cis-regulatory-architecture
#8
R Lapadat, R L Debiasi, G L Johnson, K L Tyler, I Shah
The availability of complete genomes and global gene expression profiling has greatly facilitated analysis of complex genetic regulatory systems. We describe the use of a bioinformatics strategy for analyzing the cis-regulatory design of genes diferentially regulated during viral infection of a target cell. The large-scale transcriptional activity of human embryonic kidney (HEK293) cells to reovirus (serotype 3 Abney) infection was measured using the Affymetrix HU-95Av2 gene array. Comparing the 2000 base pairs of 5' upstream sequence for the most differentially expressed genes revealed highly preserved sequence regions, which we call "modules"...
2005: Current Genomics
https://www.readbyqxmd.com/read/23099836/-unknown-significance-monoclonal-gammopathy-usmg-and-primary-hyperparathyroidism-ph-on-diferential-diagnosis-of-hypercalcemia-case-report
#9
Cristianne da Silva Alexandre, Fabyan Esberard Beltrão, Gilson Espínola Guedes Filho, Sâmia Marques Lourenço, Nardell José da Costa Freitas, Raniere Nobre Fonseca
The monoclonal gammopathy of undetermined significance (USMG) is a pre-malignant rare asymptomatic disease, defined by a concentration of immunoglobulin in serum monoclonal more than 3 g/dL and a proportion of plasma cells in bone marrow more than 10% in the absence of lytic bone lesions, anemia, hypercalcemia and renal failure related to monoclonal cell proliferation. Primary hyperparathyroidism (PH) is a relatively frequent disease, affecting approximately one in 1,000 individuals. Some studies suggest that the frequency of PH is increased in cancer, broadening the spectrum of etiology of hypercalcemia in these patients...
September 2012: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/22174987/cell-type-specific-differentiation-and-molecular-profiles-in-skin-transplantation-implication-of-medical-approach-for-genetic-skin-diseases
#10
Noritaka Oyama, Fumio Kaneko
Skin is highly accessible and valuable organ, which holds promise to accelerate the understanding of future medical innovation in association with skin transplantation, engineering, and wound healing. In skin transplantation biology, multistage and multifocal damages occur in both grafted donor and perilesional host skin and need to be repaired properly for the engraftment and maintenance of characteristic skin architecture. These local events are more unlikely to be regulated by the host immunity, because human skin transplantation has accomplished the donor skin engraftment onto the immunocompromised or immunosuppressive animals...
2011: Journal of Transplantation
https://www.readbyqxmd.com/read/21812117/evaluation-of-a-multilayered-chitosan-hydroxy-apatite-porous-composite-enriched-with-fibronectin-or-an-in-vitro-generated-bone-like-extracellular-matrix-on-proliferation-and-diferentiation-of-osteoblasts
#11
M S Fernández, J I Arias, M J Martínez, L Saenz, A Neira-Carrillo, M Yazdani-Pedram, J L Arias
The use of extracellular matrix (ECM) molecules from tissues is an interesting way to induce specific responses of cells grown onto composite scaffolds to promote adhesion, proliferation and differentiation. There have been several studies on the effects on cell proliferation and differentiation of osteoprogenitor cells cultured onto composites, either adding some ECM molecules or grown in the presence of growth factors. Other studies involve the use of osteoblasts cultured on a three-dimensional (3D) matrix, enriched with ECM molecules produced by the same cells grown previously inside the composite...
June 2012: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/21512681/-therapeutic-approach-of-differentiated-thyroid-carcinoma-in-children-and-adolescents
#12
REVIEW
Kalliopi Pazaitou-Panayiotou
Diferentiated thyroid cancer is uncommon in children and adolescents. It presents as a solitary thyroid nodule, as multinodular goiter, or as a neck mass that is noted accidentally by the patient, the family or by the physician. Surgery is the treatment of choice and most of the specialists consider that total thyroidectomy with central cervical lymph nodes dissection must be applied in all patients. Some specialists prefer a more conservative surgical treatment in order to avoid complications. Postoperatively, ablation treatment with iodine-131, for the eradication of the remaining normal or malignant thyroid tissue is indicated in the majority of patients...
January 2011: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/21222206/adult-stem-cel-diferentiation-and-trafficking-and-their-implications-in-disease
#13
Ying Zhuge, Zhao-Jun Liu, Omaida C Velazquez
Stem cells are unspecialized precursor cells that mainly reside in the bone marrow and have important roles in the establishment of embryonic tissue. They also have critical functions during adulthood, where they replenish short-lived mature effector cells and regeneration of injured tissue. They have three main characteristics: self-renewal, differentiation and homeostatic control. In order to maintain a pool of stem cells that support the production of blood cells, stromal elements and connective tissue, stem cells must be able to constantly replenish their own number...
2010: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/21222201/alternative-splicing-in-stem-cell-self-renewal-and-diferentiation
#14
REVIEW
David A Nelles, Gene W Yeo
This chapter provides a review of recent advances in understanding the importance of alternative pre-messenger RNA splicing in stem cell biology. The majority of transcribed pre-mRNAs undergo RNA splicing where introns are excised and exons are juxtaposed to form mature messenger RNA sequences. This regulated, selective removal of whole or portions of exons by alternative splicing provides avenues for control of RNA abundance and proteome diversity. We discuss several examples of key alternative splicing events in stem cell biology and provide an overview of recently developed microarray and sequencing technologies that enable systematic and genome-wide assessment of the extent of alternative splicing during stem cell differentiation...
2010: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/21179699/-biological-characteristics-of-cd133-subpopulation-in-tongue-squamous-cell-carcinoma-tca8113-cell-line
#15
Fei-wu Kang, Kai Wang, Min Wu, Zuo-lin Wang, Yan Zhu, Rui Min
OBJECTIVE: To detect the expression of CD133 in human tongue squamous cell carcinoma Tea8113 cell line and observe proliferation ability of CD133 groups in vitro. METHODS: Limiting dilution assay was employed to observe the proliferating character of Tca8113 single cell in vitro. The ability of growing as cancer spheroids was observed with ultra-low attachment plates. The flow cytometry was used to detect the expression of putative tumor-initiating cell marker CD133 in human tongue squamous cell carcinoma Tca8113 cell line...
October 2010: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/21061451/the-prenylflavonoid-phytoestrogens-8-prenylnaringenin-and-isoxanthohumol-diferentially-suppress-steroidogenesis-in-rat-leydig-cells-in-ontogenesis
#16
Gaia Izzo, Olle Söder, Konstantin Svechnikov
8-Prenylnaringenin and isoxanthohumol are prenylflavonoids found in the hop plant, Humulus lupulus (Cannabaceae), which is traditionally used to add bitterness and flavor to beer. Flavonoids have previously been reported to exert endocrine disrupting actions. Therefore, we investigated the effects of 8-prenylnaringenin and isoxanthohumol on steroidogenesis activated by human chorionic gonadotropin (hCG) in primary cultures of rat Leydig cells at different stages of their development. The present study is the first to demonstrate that the prenylflavonoids 8-prenylnaringenin and isoxanthohumol exert complex maturation-dependent effects on Leydig cell steroidogenesis...
August 2011: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/20681317/biology-and-applications-of-mesenchymal-stem-cells
#17
REVIEW
Pedro Cesar Chagastelles, Nance Beyer Nardi, Melissa Camassola
Undifferentiated adult stem cells are responsible for cell replacement in adult organisms. Initially isolated from the bone marrow, they are now known to be distributed throughout the organism as a whole, with a perivascular location. They are defined by properties which include proliferation as adherent cells, a defined immunophenotype, and the capacity to differentiate in vitro into osteoblasts, adipocytes and chondroblasts. Mesenchymal stem cells (MSCs) are considered as one of the most promising cell types for therapeutic applications...
2010: Science Progress
https://www.readbyqxmd.com/read/20369921/spatially-distributed-morphogen-production-and-morphogen-gradient-formation
#18
Arthur D Lander, Qing Nie, Frederic Y M Wan
Partial diferential equations and auxiliary conditions governing the activities of the morphogen Dpp in Drosophila wing imaginal discs were formulated and analyzed as Systems B, R, and C in [7] [9] [10]. All had morphogens produced at the border of anterior and posterior chamber of the wing disc idealized as a point, line, or plane in a one-, two-, or three-dimensional model. In reality, morphogens are synthesized in a narrow region of finite width (possibly of only a few cells) between the two chambers in which diffusion and reversible binding with degradable receptors may also take place...
April 2005: Mathematical Biosciences and Engineering: MBE
https://www.readbyqxmd.com/read/19883011/-cell-therapy-results-in-cardiology
#19
Philippe Menasché
Cell therapy is already a clinical reality, having restored function to postinfarct akinetic myocardial scars. Ongoing trials are testing skeletal myoblasts in patients with chronic left ventricular dysfunction, and bone marrow-derived cells are being tried in patients with acute myocardial infarction undergoing concomitant percutaneous revascularization by angioplasty and stenting. While these procedures appear to be safe, their efficacy is uncertain. Indeed, the enthusiasm generated by the first phase I studies has been tempered by the less successful outcomes of recently published randomised controlled phase II trials...
March 2009: Bulletin de L'Académie Nationale de Médecine
https://www.readbyqxmd.com/read/19177856/oral-and-oropharyngeal-cancer-in-a-venezuelan-population
#20
Helen Rivera, Nikolaos G Nikitakis, María Correnti, Sara Maissi, José G Ponce
The aim of this work was to analyze diagnosed cases of Oral Cancer (OC) and Oropharyngeal Cancer (OPC) in a Venezuelan population. We clinically evaluated 130 patients with OC and OPC and a histopathologic diagnosis of squamous cell carcinoma. The patients were analyzed according to gender age, and use of alcohol and tobacco and the tumors were classIfied based on anatomic location, staging parameters, and degree of difFerentiation. Ninety one patients (70%) were male and 39 (30%) were female. Patients' age ranged from 26 to 86 years old...
2008: Acta Odontológica Latinoamericana: AOL
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