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https://www.readbyqxmd.com/read/28804955/3m-052-as-an-adjuvant-for-a-plga-microparticle-based-leishmania-donovani-recombinant-protein-vaccine
#1
Qian Wang, Meagan A Barry, Christopher A Seid, Elissa M Hudspeth, C Patrick McAtee, Michael J Heffernan
It is believed that an effective vaccine against leishmaniasis will require a T helper type 1 (TH 1) immune response. In this study, we investigated the adjuvanticity of the Toll-like receptor (TLR) 7/8 agonist 3M-052 in combination with the Leishmania donovani 36-kDa nucleoside hydrolase recombinant protein antigen (NH36). NH36 and 3M-052 were encapsulated in separate batches of poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs). The loading efficiency for NH36 was 83% and for 3M-052 was above 95%. In vitro stimulation of bone marrow-derived dendritic cells, measured by IL-12 secretion, demonstrated that 3M-052 (free or MP-formulated) had a concentration-dependent immunostimulatory effect with an optimum concentration of 2 µg/mL...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28797967/multipurpose-tenofovir-disoproxil-fumarate-electrospun-fibers-for-the-prevention-of-hiv-1-and-hsv-2-infections-in-vitro
#2
Kevin M Tyo, Hung R Vuong, Danial A Malik, Lee B Sims, Houda Alatassi, Jinghua Duan, Walter H Watson, Jill M Steinbach-Rankins
Sexually transmitted infections affect hundreds of millions of people worldwide. Both human immunodeficiency virus (HIV-1 and -2) and herpes simplex virus-2 (HSV-2) remain incurable, urging the development of new prevention strategies. While current prophylactic technologies are dependent on strict user adherence to achieve efficacy, there is a dearth of delivery vehicles that provide discreet and convenient administration, combined with prolonged-delivery of active agents. To address these needs, we created electrospun fibers (EFs) comprised of FDA-approved polymers, poly(lactic-co-glycolic acid) (PLGA) and poly(DL-lactide-co-ε-caprolactone) (PLCL), to provide sustained-release and in vitro protection against HIV-1 and HSV-2...
August 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28793048/development-of-vegf-loaded-plga-matrices-in-association-with-mesenchymal-stem-cells-for-tissue-engineering
#3
A R Rosa, D Steffens, B Santi, K Quintiliano, N Steffen, D A Pilger, P Pranke
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured...
August 7, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28746182/plumbagin-loaded-aptamer-targeted-poly-d-l-lactic-co-glycolic-acid-b-polyethylene-glycol-nanoparticles-for-prostate-cancer-therapy
#4
Minjie Pan, Weifeng Li, Jun Yang, Zhiqin Li, Jun Zhao, Yajun Xiao, Yifei Xing, Xiaoping Zhang, Wen Ju
Plumbagin inhibits the growth, metastasis, and invasion of prostate cancer (PCa). However, its lower bioavailability limits biopharmaceutical properties due to insolubility in water. Prostate-specific membrane antigen (PSMA) aptamer-targeted nanoparticles (NPs) significantly enhanced cytotoxicity in prostate epithelial cells. This study aimed to investigate the effects of plumbagin-loaded prostate-specific membrane antigen (PSMA) aptamer-targeted poly D,L-lactic-co-glycolic acid-b-polyethylene glycol (PLGA-PEG) nanoparticles (NPs) on prostate cancer (PCa) in vitro...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28742842/new-hydrazonoindolin-2-ones-synthesis-exploration-of-the-possible-anti-proliferative-mechanism-of-action-and-encapsulation-into-plga-microspheres
#5
Mohamed I Attia, Wagdy M Eldehna, Samar A Afifi, Adam B Keeton, Gary A Piazza, Hatem A Abdel-Aziz
The synthesis and molecular characterization of new isatin-based hydrazonoindolin-2-ones 4a-o and 7a-e are reported. The in vitro anti-proliferative potential of the synthesized compounds 4a-o and 7a-e was examined against HT-29 (colon), ZR-75 (breast) and A549 (lung) human cancer cell lines. Compounds 7b, 7d and 7e were the most active congeners against the tested human cancer cell lines with average IC50 values of 4.77, 3.39 and 2.37 μM, respectively, as compared with the reference isatin-based drug, sunitinib, which exhibited an average IC50 value of 8...
2017: PloS One
https://www.readbyqxmd.com/read/28736613/cd200-modulates-macrophage-cytokine-secretion-and-phagocytosis-in-response-to-poly-lactic-co-glycolic-acid-microparticles-and-films
#6
E Y Chen, S Chu, L Gov, Y K Kim, M B Lodoen, A J Tenner, W F Liu
Biocompatibility is a major concern for developing biomaterials used in medical devices, tissue engineering and drug delivery. Poly(lactic-co-glycolic acid) (PLGA) is one of the most widely used biodegradable materials, yet still triggers a significant foreign body response that impairs healing. Immune cells including macrophages respond to the implanted biomaterial and mediate the host response, which can eventually lead to device failure. Previously in our laboratory, we found that CD200, an immunomodulatory protein, suppressed macrophage inflammatory activation in vitro and reduced local immune cell infiltration around a biomaterial implant...
February 28, 2017: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/28732712/structure-performance-relationships-of-temperature-responsive-plga-peg-plga-gels-for-sustained-release-of-bone-morphogenetic-protein-2
#7
Ana Santoveña, Cecilia Monzón, Carmen Alvarez-Lorenzo, Carlos Del Rosario, Araceli Delgado, Carmen Evora, Angel Concheiro, Matias Llabrés, José B Fariña
PLGA (poly(lactic-co-glycolic) acid)-PEG (polyethylene glycol)-PLGA synthesis conditions have an impact on the physicochemical features of the copolymer and its usefulness as biomaterial. This study reports on an analysis of the composition and structural properties of PLGA-PEG-PLGA copolymers applying a variety of analytical techniques. Viscoelastic properties and particularly the temperature-responsive behavior of PLGA-PEG-PLGA showed a marked dependence on copolymer structural features. Physicochemical and biological properties, such as bioadhesion, biocompatibility and cell viability, of the raw copolymers and their gels were also evaluated...
July 19, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28732229/a-novel-approach-to-fabricate-dye-encapsulated-polymeric-micro-and-nanoparticles-by-thin-film-dewetting-technique
#8
Manosree Chatterjee, Abhiram Hens, Kuldeep Mahato, Namita Jaiswal, Nivedita Mahato, Nagahanumaiah, Nripen Chanda
A new method is reported for fabrication of polymeric micro- and nanoparticles from an intermediate patterned surface originated by dewetting of a polymeric thin film. Poly (d, l-lactide-co-glycolide) or PLGA, a biocompatible polymer is used to develop a thin film over a clean glass substrate which dewets spontaneously in the micro-/nano-patterned surface of size range 50nm to 3.5µm. Since another water-soluble polymer, poly vinyl alcohol (PVA) is coated on the same glass substrate before PLGA thin film formation, developed micro-/nano-patterns are easily extracted in water in the form of micro- and nanoparticle mixture of size range 50nm to 3...
July 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28728978/anticancer-drug-loaded-quantum-dots-engineered-polymeric-nanoparticles-diagnosis-therapy-combined-approach
#9
D Belletti, G Riva, M Luppi, G Tosi, F Forni, M A Vandelli, B Ruozi, F Pederzoli
Primary Effusion Lymphoma (PEL) is an HHV-8-related non Hodgkin lymphoma localized in body cavities (as pleural, peritoneal and pericardial) presenting lymphomatous effusion that, until now, lack of an effective therapy. Curcumin was reported to display pro-apoptotic effect via the inhibition of the JAK/STAT pathway, that is overexpressed in PEL cells, as consequence of virus infection. The administration of curcumin is severely restricted by its physicochemical properties, mainly its low solubility in biological fluid and consequently low bioavailability...
July 18, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28713741/reconstruction-of-drug-induced-cleft-palate-using-bone-marrow-mesenchymal-stem-cell-in-rodents
#10
Julie Christy Amalraj, Manasa Gangothri, Hari Babu
BACKGROUND: Triamcinolone acetonide (TAC) (Kenacort*) is a commonly used synthetic glucocorticoid in today's medical practice. The drug is also a potential agent in inducing cleft palates in rats. This drug has been used to induce cleft palate in the fetus of the pregnant rats to bring out a suitable animal model for human cleft lip and palate. The drug was given intraperitoneally to induce congenital cleft palate in pregnant mother rats. AIM: The aim of this study is to induce congenital cleft palate in pregnant Wister albino rats and reconstruct the defect with bone marrow mesenchymal stem cells (BMSCs) isolated from the same species along with PLGA (poly lactic co glycolic acid) scaffold...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28649247/an-antigen-presenting-and-apoptosis-inducing-polymer-microparticle-prolongs-alloskin-graft-survival-by-selectively-and-markedly-depleting-alloreactive-cd8-t-cells
#11
Wei Wang, Khawar Ali Shahzad, Miaochen Li, Aifeng Zhang, Lei Zhang, Tao Xu, Xin Wan, Chuanlai Shen
Selectively depleting the pathogenic T cells is a fundamental strategy for the treatment of allograft rejection and autoimmune disease since it retains the overall immune function of host. The concept of killer artificial antigen-presenting cells (KaAPCs) has been developed by co-coupling peptide-major histocompatibility complex (pMHC) multimer and anti-Fas monoclonal antibody (mAb) onto the polymeric microparticles (MPs) to induce the apoptosis of antigen-specific T cells. But little information is available about its in vivo therapeutic potential and mechanism...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28619603/water-soluble-combretastatin-a4-phosphate-orally-delivered-via-composite-nanoparticles-with-improved-inhibition-effect-toward-s180-tumors
#12
Yurun Shen, Liping Wu, Liyan Qiu
Combretastatin A4 phosphate (CA4P) is a novel vascular disrupting agent for cancer therapy. However, frequent dosing and negative patient compliance have been encountered over CA4P by injection administration due to its quite short-term action and acute side effects. Therefore, it is significant to develop an oral formulation of CA4P. We established a novel method to prepare CA4P-loaded nanoparticles (CA4P-NPs) for oral administration by combining methoxy poly(ethylene glycol)-b-polylactide (PELA) and poly(d,l-lactic-co-glycolic acid) (PLGA) polymers...
June 13, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28617560/influence-of-vegf-bmp-2-on-the-proliferation-and-osteogenetic-differentiation-of-rat-bone-mesenchymal-stem-cells-on-plga-gelatin-composite-scaffold
#13
G An, W-B Zhang, D-K Ma, B Lu, G-J Wei, Y Guang, C-H Ru, Y-S Wang
OBJECTIVE: To investigate the influence of VEGF/BMP-2 on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells BMSCs) on PLGA/gelatin composite scaffold. MATERIALS AND METHODS: Randomly-oriented nanofibers with different ratios of Poly Lactic-co-Glycolic Acid (PLGA)/gelatin were produced through electrospinning. The mixture of nanofibers and BMSCs was pipetted onto the surface of the scaffolds, and BMSCs/PLGA/gelatin composite was obtained...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28591594/oleanolic-bioenhancer-coloaded-chitosan-modified-nanocarriers-attenuate-breast-cancer-cells-by-multimode-mechanism-and-preserve-female-fertility
#14
Monika Sharma, Shweta Sharma, Vikas Sharma, Komal Sharma, Santosh Kumar Yadav, Pankaj Dwivedi, Satish Agrawal, Sarvesh Kumar Paliwal, Anil Kumar Dwivedi, Jagdamba Prasad Maikhuri, Gopal Gupta, Prabhat Ranjan Mishra, Ajay Kumar Singh Rawat
Addressing multidrug resistant stage of breast cancer is an impediment for chemotherapy. Moreover, breast cancer chemotherapy has potential enduring confrontations i.e. related toxicity including effect on fertility of young female patients. The co-delivery of polyphenolic bio-enhancers with oleanolic acid in chitosan coated PLGA nanoparticles was designed for oral delivery with enhanced antitumor effect consecutively preserving the female fertility. The optimized oleanolic- bio-enhancer nano formulation CH-OA-B-PLGA with particle size was 342...
June 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28549089/development-of-a-sustained-release-voriconazole-containing-thermogel-for-subconjunctival-injection-in-horses
#15
Rosemary S Cuming, Eva M Abarca, Sue Duran, Anne A Wooldridge, Allison J Stewart, William Ravis, R Jayachandra Babu, Yuh-Jing Lin, Terri Hathcock
Purpose: To determine in vitro release profiles, transcorneal permeation, and ocular injection characteristics of a voriconazole-containing thermogel suitable for injection into the subconjunctival space (SCS). Methods: In vitro release rate of voriconazole (0.3% and 1.5%) from poly (DL-lactide-co-glycolide-b-ethylene glycol-b-DL-lactide-co-glycolide) (PLGA-PEG-PLGA) thermogel was determined for 28 days. A Franz cell diffusion chamber was used to evaluate equine transcorneal and transscleral permeation of voriconazole (1...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28542454/controlled-delivery-of-tauroursodeoxycholic-acid-from-biodegradable-microspheres-slows-retinal-degeneration-and-vision-loss-in-p23h-rats
#16
Laura Fernández-Sánchez, Irene Bravo-Osuna, Pedro Lax, Alicia Arranz-Romera, Victoria Maneu, Sergio Esteban-Pérez, Isabel Pinilla, María Del Mar Puebla-González, Rocío Herrero-Vanrell, Nicolás Cuenca
Successful drug therapies for treating ocular diseases require effective concentrations of neuroprotective compounds maintained over time at the site of action. The purpose of this work was to assess the efficacy of intravitreal controlled delivery of tauroursodeoxycholic acid (TUDCA) encapsulated in poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres for the treatment of the retina in a rat model of retinitis pigmentosa. PLGA microspheres (MSs) containing TUDCA were produced by the O/W emulsion-solvent evaporation technique...
2017: PloS One
https://www.readbyqxmd.com/read/28501941/paracrine-release-of-il-2-and-anti-ctla-4-enhances-the-ability-of-artificial-polymer-antigen-presenting-cells-to-expand-antigen-specific-t-cells-and-inhibit-tumor-growth-in-a-mouse-model
#17
Lei Zhang, Limin Wang, Khawar Ali Shahzad, Tao Xu, Xin Wan, Weiya Pei, Chuanlai Shen
Accumulating evidence indicates that bead-based artificial antigen-presenting cells (aAPCs) are a powerful tool to induce antigen-specific T cell responses in vitro and in vivo. To date, most conventional aAPCs have been generated by coupling an antigen signal (signal 1) and one or two costimulatory signals, such as anti-CD28 with anti-LFA1 or anti-4-1BB (signal 2), onto the surfaces of cell-sized or nanoscale magnetic beads or polyester latex beads. The development of a biodegradable scaffold and the combined use of multiple costimulatory signals as well as third signals for putative clinical applications is the next step in the development of this technology...
May 13, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28429764/fabrication-characterization-and-biological-evaluation-of-anti-her2-indocyanine-green-doxorubicin-encapsulated-peg-b-plga-copolymeric-nanoparticles-for-targeted-photochemotherapy-of-breast-cancer-cells
#18
Yu-Hsiang Lee, Da-Sheng Chang
In this study, we aimed to develop anti-human epidermal growth factor receptor 2 (HER2) indocyanine green (ICG)-doxorubicin (DOX)-encapsulated polyethylene glycol-poly(lactic-co-glycolic acid) diblock copolymeric nanoparticles (HIDPPNPs) to explore the co-administration of phototherapy and chemotherapy for HER2-overexpressing breast cancer, a highly aggressive and medicine-resistant breast carcinoma. The HIDPPNPs were fabricated using a solvent evaporation technique followed by carbodiimide-mediated antibody conjugation on the nanoparticle surface...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28367618/regulation-of-drug-release-by-tuning-surface-textures-of-biodegradable-polymer-microparticles
#19
Mubashir Hussain, Jun Xie, Zaiyan Hou, Khurram Shezad, Jiangping Xu, Ke Wang, Yujie Gao, Lei Shen, Jintao Zhu
Generally, size, uniformity, shape, and surface chemistry of biodegradable polymer particles will significantly affect the drug-release behavior in vitro and in vivo. In this study, uniform poly(d,l-lactic-co-glycolide) (PLGA) and PLGA-b-poly(ethylene glycol) (PLGA-b-PEG) microparticles with tunable surface textures were generated by combining the interfacial instabilities of emulsion droplet and polymer-blending strategy. Monodisperse emulsion droplets containing polymers were generated through the microfluidic flow-focusing technique...
April 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28319404/encapsulation-of-an-ep67-conjugated-ctl-peptide-vaccine-in-nanoscale-biodegradable-particles-increases-the-efficacy-of-respiratory-immunization-and-affects-the-magnitude-and-memory-subsets-of-vaccine-generated-mucosal-and-systemic-cd8-t-cells-in-a-diameter
#20
Bala V K Karuturi, Shailendra B Tallapaka, Pravin Yeapuri, Stephen M Curran, Sam D Sanderson, Joseph A Vetro
The diameter of biodegradable particles used to coencapsulate immunostimulants and subunit vaccines affects the magnitude of memory CD8(+) T cells generated by systemic immunization. Possible effects on the magnitude of CD8(+) T cells generated by mucosal immunization or memory subsets that potentially correlate more strongly with protection against certain pathogens, however, are unknown. In this study, we conjugated our novel host-derived mucosal immunostimulant, EP67, to the protective MCMV CTL epitope, pp89, through a lysosomal protease-labile double arginine linker (pp89-RR-EP67) and encapsulated in PLGA 50:50 micro- or nanoparticles...
April 3, 2017: Molecular Pharmaceutics
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